Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29359.t1 | XP_003601259.1 | 99.4 | 894 | 3 | 2 | 1 | 893 | 1 | 893 | 0.00E+00 | 1759.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29359.t1 | Q8L5Y9 | 85.9 | 871 | 117 | 5 | 28 | 893 | 31 | 900 | 0.0e+00 | 1495.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29359.t1 | G7J5F4 | 99.4 | 894 | 3 | 2 | 1 | 893 | 1 | 893 | 0.0e+00 | 1759.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049264 | MS.gene29359 | 0.841353 | 5.00E-58 | -1.69E-46 |
| MS.gene049378 | MS.gene29359 | 0.874312 | 7.45E-68 | -1.69E-46 |
| MS.gene04991 | MS.gene29359 | -0.821404 | 4.11E-53 | -1.69E-46 |
| MS.gene050226 | MS.gene29359 | 0.804641 | 1.96E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene29359 | 0.808697 | 2.73E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene29359 | 0.806771 | 7.00E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene29359 | 0.811677 | 6.21E-51 | -1.69E-46 |
| MS.gene050762 | MS.gene29359 | 0.825739 | 3.98E-54 | -1.69E-46 |
| MS.gene050763 | MS.gene29359 | 0.824888 | 6.32E-54 | -1.69E-46 |
| MS.gene050826 | MS.gene29359 | 0.805092 | 1.58E-49 | -1.69E-46 |
| MS.gene051935 | MS.gene29359 | 0.808168 | 3.54E-50 | -1.69E-46 |
| MS.gene052389 | MS.gene29359 | 0.840281 | 9.56E-58 | -1.69E-46 |
| MS.gene053147 | MS.gene29359 | 0.804303 | 2.30E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene29359 | 0.828686 | 7.83E-55 | -1.69E-46 |
| MS.gene054468 | MS.gene29359 | 0.861653 | 8.82E-64 | -1.69E-46 |
| MS.gene054826 | MS.gene29359 | 0.889401 | 2.50E-73 | -1.69E-46 |
| MS.gene054930 | MS.gene29359 | 0.836727 | 7.90E-57 | -1.69E-46 |
| MS.gene055701 | MS.gene29359 | 0.809042 | 2.30E-50 | -1.69E-46 |
| MS.gene05595 | MS.gene29359 | 0.815637 | 8.35E-52 | -1.69E-46 |
| MS.gene056480 | MS.gene29359 | 0.816534 | 5.26E-52 | -1.69E-46 |
| MS.gene057026 | MS.gene29359 | 0.804666 | 1.94E-49 | -1.69E-46 |
| MS.gene05761 | MS.gene29359 | 0.828019 | 1.13E-54 | -1.69E-46 |
| MS.gene057929 | MS.gene29359 | 0.830918 | 2.24E-55 | -1.69E-46 |
| MS.gene058651 | MS.gene29359 | 0.84064 | 7.70E-58 | -1.69E-46 |
| MS.gene05872 | MS.gene29359 | 0.821975 | 3.03E-53 | -1.69E-46 |
| MS.gene060373 | MS.gene29359 | 0.827369 | 1.63E-54 | -1.69E-46 |
| MS.gene06052 | MS.gene29359 | 0.820845 | 5.53E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29359.t1 | MTR_3g077740 | 99.441 | 894 | 3 | 2 | 1 | 893 | 1 | 893 | 0.0 | 1823 |
| MS.gene29359.t1 | MTR_8g064850 | 61.463 | 410 | 148 | 3 | 45 | 453 | 1 | 401 | 2.43e-172 | 506 |
| MS.gene29359.t1 | MTR_8g064850 | 62.751 | 349 | 120 | 3 | 45 | 392 | 1 | 340 | 1.63e-148 | 442 |
| MS.gene29359.t1 | MTR_1g076720 | 36.269 | 386 | 201 | 10 | 519 | 890 | 11 | 365 | 1.53e-67 | 229 |
| MS.gene29359.t1 | MTR_6g060550 | 35.825 | 388 | 205 | 11 | 519 | 893 | 10 | 366 | 3.08e-66 | 226 |
| MS.gene29359.t1 | MTR_1g076730 | 36.829 | 391 | 203 | 10 | 513 | 889 | 158 | 518 | 3.17e-65 | 228 |
| MS.gene29359.t1 | MTR_6g060550 | 33.721 | 344 | 185 | 10 | 519 | 849 | 51 | 364 | 5.27e-47 | 173 |
| MS.gene29359.t1 | MTR_6g060550 | 33.724 | 341 | 183 | 10 | 519 | 846 | 10 | 320 | 6.29e-47 | 171 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29359.t1 | AT4G32180 | 85.878 | 871 | 117 | 5 | 28 | 893 | 31 | 900 | 0.0 | 1518 |
| MS.gene29359.t1 | AT4G32180 | 86.321 | 753 | 98 | 4 | 28 | 776 | 31 | 782 | 0.0 | 1333 |
| MS.gene29359.t1 | AT4G32180 | 86.321 | 753 | 98 | 4 | 28 | 776 | 31 | 782 | 0.0 | 1333 |
| MS.gene29359.t1 | AT1G60440 | 62.245 | 392 | 124 | 6 | 66 | 455 | 3 | 372 | 1.11e-146 | 439 |
| MS.gene29359.t1 | AT1G60440 | 70.790 | 291 | 83 | 2 | 166 | 455 | 6 | 295 | 3.63e-128 | 388 |
| MS.gene29359.t1 | AT1G60440 | 55.584 | 385 | 113 | 5 | 72 | 455 | 6 | 333 | 1.85e-119 | 367 |
| MS.gene29359.t1 | AT2G17320 | 37.500 | 368 | 197 | 10 | 525 | 890 | 21 | 357 | 8.38e-70 | 236 |
| MS.gene29359.t1 | AT4G35360 | 38.655 | 357 | 186 | 10 | 536 | 890 | 38 | 363 | 2.70e-69 | 234 |
| MS.gene29359.t1 | AT4G35360 | 38.655 | 357 | 186 | 10 | 536 | 890 | 38 | 363 | 2.70e-69 | 234 |
| MS.gene29359.t1 | AT2G17340 | 37.772 | 368 | 188 | 10 | 524 | 889 | 34 | 362 | 1.39e-68 | 233 |
| MS.gene29359.t1 | AT2G17320 | 37.861 | 346 | 183 | 9 | 525 | 868 | 21 | 336 | 3.58e-64 | 220 |
| MS.gene29359.t1 | AT4G35360 | 41.463 | 287 | 138 | 7 | 605 | 890 | 9 | 266 | 6.82e-63 | 214 |
Find 218 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAACCATCCTTACAATTT+TGG | 0.057026 | 3.3:-65957809 | None:intergenic |
| CAATTTGAACAAACTCTTTC+TGG | 0.085671 | 3.3:-65955448 | None:intergenic |
| TAGCTCTATTATCTCCTTTC+TGG | 0.143633 | 3.3:-65955838 | None:intergenic |
| TATAGCATTGTGACATTCTA+AGG | 0.194825 | 3.3:+65962906 | MS.gene29359:intron |
| TTTGCGACATGAAGGATTTC+TGG | 0.197061 | 3.3:+65956683 | MS.gene29359:CDS |
| TCCACTATTGCTTCAAGTTT+TGG | 0.201193 | 3.3:+65956083 | MS.gene29359:CDS |
| GTGCTATTTGCAGATAATTT+TGG | 0.221515 | 3.3:+65963186 | MS.gene29359:intron |
| GAAGGATTTCTGGGAGCTTT+AGG | 0.237803 | 3.3:+65956693 | MS.gene29359:CDS |
| GATAAGGGAACAAATCATTT+TGG | 0.246674 | 3.3:-65955472 | None:intergenic |
| TTGGCTGAAAAGTTGATTAA+AGG | 0.257846 | 3.3:+65963909 | MS.gene29359:CDS |
| TCTCATCTCGCTCTTGATAT+TGG | 0.262842 | 3.3:+65953039 | MS.gene29359:CDS |
| TTACTGTTTGTTGATAATTC+AGG | 0.264371 | 3.3:+65961213 | MS.gene29359:CDS |
| TCAGGTGCTGACATTGTTCT+AGG | 0.266086 | 3.3:+65961231 | MS.gene29359:CDS |
| AGGAGAAGCTTTCCTATTCT+TGG | 0.278927 | 3.3:+65954048 | MS.gene29359:CDS |
| TGGTGGTTTGGATTATTCTA+AGG | 0.289539 | 3.3:+65955886 | MS.gene29359:CDS |
| ACTCAAAGTTACCTTAATTA+TGG | 0.294989 | 3.3:-65954349 | None:intergenic |
| CCTCTGCTTTATACTTCTAT+AGG | 0.299214 | 3.3:-65960190 | None:intergenic |
| ATGTTGGTGGTGGTACTTAT+TGG | 0.301273 | 3.3:+65955675 | MS.gene29359:CDS |
| ATGCTTGTTGGGGACATTTA+TGG | 0.301696 | 3.3:+65955866 | MS.gene29359:CDS |
| TGTTGGTGGTGGTACTTATT+GGG | 0.303285 | 3.3:+65955676 | MS.gene29359:CDS |
| GCCAATCAGACGATGATAAA+AGG | 0.305514 | 3.3:+65953983 | MS.gene29359:CDS |
| CCCTTGATGGTTGTTGAGAA+TGG | 0.311987 | 3.3:+65963007 | MS.gene29359:CDS |
| AAAAGCATCACCCCTTCTTT+TGG | 0.318392 | 3.3:-65959323 | None:intergenic |
| GTTAGGCTTGGCCAATCTAT+TGG | 0.318807 | 3.3:+65960125 | MS.gene29359:CDS |
| TGTATCTTGCCACCAGTATA+TGG | 0.319902 | 3.3:-65956795 | None:intergenic |
| TGGGGACATTTATGGTGGTT+TGG | 0.324370 | 3.3:+65955874 | MS.gene29359:CDS |
| TTTCATTTGCTGTTCAATAC+TGG | 0.329419 | 3.3:+65956533 | MS.gene29359:CDS |
| GGGAAGTTTGAGAGGGTTAG+TGG | 0.335778 | 3.3:+65955647 | MS.gene29359:CDS |
| TGATCGAAATGCCATAATTA+AGG | 0.338567 | 3.3:+65954338 | MS.gene29359:CDS |
| CTTCTAGTTAATGTTGGTTC+CGG | 0.339897 | 3.3:+65955494 | MS.gene29359:CDS |
| AGGAGCAAATTTCTTGCTTA+AGG | 0.343687 | 3.3:+65954839 | MS.gene29359:CDS |
| CACTTTGTCAAATTTGAAAC+GGG | 0.349017 | 3.3:+65954081 | MS.gene29359:CDS |
| CTCTTCAAAGAGAGAATGTT+AGG | 0.349289 | 3.3:+65961153 | MS.gene29359:CDS |
| GCAGCCTCAGCTACAATATC+AGG | 0.349938 | 3.3:-65961945 | None:intergenic |
| TTCATCTTCGGTCCCCTTGA+TGG | 0.350155 | 3.3:+65962994 | MS.gene29359:CDS |
| AAACATTGAAGGAGCGATTA+GGG | 0.351661 | 3.3:+65954010 | MS.gene29359:CDS |
| TAGGGCTTTCTAATGGTAAC+AGG | 0.355069 | 3.3:+65954028 | MS.gene29359:CDS |
| TTCCCTCATTTCCAATAGAT+TGG | 0.358243 | 3.3:-65960136 | None:intergenic |
| ACTCCTGTTGCTGGAACTAC+TGG | 0.372736 | 3.3:+65957665 | MS.gene29359:CDS |
| AGAGAGAGTGATATGTCTTC+AGG | 0.373424 | 3.3:-65956146 | None:intergenic |
| TTCTATAGGCATCAACAAAC+TGG | 0.374707 | 3.3:-65960176 | None:intergenic |
| GTGCCGATGACTCCTGTTGC+TGG | 0.376588 | 3.3:+65957656 | MS.gene29359:CDS |
| AAAACATTGAAGGAGCGATT+AGG | 0.380222 | 3.3:+65954009 | MS.gene29359:CDS |
| CCTTCTGCATACGGCAAGTT+AGG | 0.380263 | 3.3:+65960108 | MS.gene29359:CDS |
| GAGCTATCGACATGCTTGTT+GGG | 0.380873 | 3.3:+65955855 | MS.gene29359:CDS |
| CACCAAGTCTTTCTTTGAAA+AGG | 0.381637 | 3.3:-65954776 | None:intergenic |
| GTTTGTAGAACTTGATAGTA+TGG | 0.382267 | 3.3:+65960354 | MS.gene29359:CDS |
| TGCTGCAAAAGACGCCGATT+TGG | 0.388650 | 3.3:+65963081 | MS.gene29359:CDS |
| GTTTAAGTGTGATGCTTTGA+AGG | 0.388883 | 3.3:+65963249 | MS.gene29359:CDS |
| GAACAACAACAACAAGTTCT+TGG | 0.391161 | 3.3:+65952844 | MS.gene29359:CDS |
| TGGTGGTACTTATTGGGGAC+TGG | 0.395806 | 3.3:+65955682 | MS.gene29359:CDS |
| ATTTGCTTACTCTAATTCAC+TGG | 0.397587 | 3.3:-65958284 | None:intergenic |
| CCGATGGGTGCTCCATATAC+TGG | 0.398412 | 3.3:+65956783 | MS.gene29359:CDS |
| TTTGGAAGTGTTTCCGTTGT+TGG | 0.401244 | 3.3:+65957781 | MS.gene29359:CDS |
| TGTTCTAGGGATGCTTCCTC+TGG | 0.402559 | 3.3:+65961245 | MS.gene29359:CDS |
| AAGACAGGTCAGCTCGGAAT+TGG | 0.403885 | 3.3:+65963054 | MS.gene29359:CDS |
| CCATGTGTGTGAAAGCTTCA+TGG | 0.404252 | 3.3:-65955421 | None:intergenic |
| TTTATTTGTCATAGGTTGAT+GGG | 0.404451 | 3.3:+65955621 | MS.gene29359:intron |
| CTTCCAAAGTTGGTACTAGA+TGG | 0.406132 | 3.3:-65957766 | None:intergenic |
| TCCAAAACTTGAAGCAATAG+TGG | 0.413680 | 3.3:-65956084 | None:intergenic |
| CTTGAACTGGATCTATGTAT+TGG | 0.418629 | 3.3:-65952925 | None:intergenic |
| CCAACATTAACTAGAAGATA+AGG | 0.421746 | 3.3:-65955488 | None:intergenic |
| TTGTCATAGGTTGATGGGGA+TGG | 0.422373 | 3.3:+65955626 | MS.gene29359:intron |
| CATCGCCTGGGCTTCTCCAT+TGG | 0.428278 | 3.3:-65956658 | None:intergenic |
| TTGCGACATGAAGGATTTCT+GGG | 0.430140 | 3.3:+65956684 | MS.gene29359:CDS |
| CCTTATCTTCTAGTTAATGT+TGG | 0.434063 | 3.3:+65955488 | MS.gene29359:CDS |
| ACTACCTTGTCCACAAGATC+TGG | 0.434369 | 3.3:-65958418 | None:intergenic |
| TCTGCAAGATCGATTGTATT+GGG | 0.438407 | 3.3:-65958259 | None:intergenic |
| TGTCATAGGTTGATGGGGAT+GGG | 0.443864 | 3.3:+65955627 | MS.gene29359:intron |
| CTAAGGCGAGCTGCTGAAGC+TGG | 0.448195 | 3.3:+65962923 | MS.gene29359:CDS |
| AGTCCAGTAGTTCCAGCAAC+AGG | 0.450308 | 3.3:-65957668 | None:intergenic |
| CCTTGATGGTTGTTGAGAAT+GGG | 0.452403 | 3.3:+65963008 | MS.gene29359:CDS |
| TTGCTGGAACTACTGGACTT+GGG | 0.454358 | 3.3:+65957672 | MS.gene29359:CDS |
| TCACTTTGTCAAATTTGAAA+CGG | 0.458203 | 3.3:+65954080 | MS.gene29359:CDS |
| ATCAAGTTGAGGCCTTGAAC+TGG | 0.458404 | 3.3:-65952938 | None:intergenic |
| ATCTGCAAGATCGATTGTAT+TGG | 0.462851 | 3.3:-65958260 | None:intergenic |
| AAGCAATAGTGGAAGCTGAT+AGG | 0.466489 | 3.3:-65956073 | None:intergenic |
| GAGCGATTAGGGCTTTCTAA+TGG | 0.468080 | 3.3:+65954021 | MS.gene29359:CDS |
| TTTGAAGTCCCTCTGTCAAA+AGG | 0.469850 | 3.3:+65957698 | MS.gene29359:CDS |
| CCCATTCTCAACAACCATCA+AGG | 0.471436 | 3.3:-65963008 | None:intergenic |
| ATCATCAGTTCCACCTTCAC+TGG | 0.473649 | 3.3:-65959299 | None:intergenic |
| AACAGTAAAGCTCTTCTATG+AGG | 0.474599 | 3.3:-65961198 | None:intergenic |
| CTCAAAAGAAAATTCATCTT+CGG | 0.475886 | 3.3:+65962982 | MS.gene29359:CDS |
| GCCTGTAGGAGCCATGTTGT+TGG | 0.477390 | 3.3:-65952899 | None:intergenic |
| TGAAGCCTTCCACCAAGAAT+AGG | 0.477732 | 3.3:-65954060 | None:intergenic |
| AGACTGCTTACTCTAATTGA+AGG | 0.478438 | 3.3:+65960895 | MS.gene29359:CDS |
| GGTGGTACTTATTGGGGACT+GGG | 0.480437 | 3.3:+65955683 | MS.gene29359:CDS |
| AGATTGATTAGGCAACAAAA+TGG | 0.482598 | 3.3:-65953007 | None:intergenic |
| GTTGCTGGAACTACTGGACT+TGG | 0.485530 | 3.3:+65957671 | MS.gene29359:CDS |
| TAGGGATGCTTCCTCTGGCT+AGG | 0.486511 | 3.3:+65961250 | MS.gene29359:CDS |
| TGCTGGAACTACTGGACTTG+GGG | 0.488456 | 3.3:+65957673 | MS.gene29359:CDS |
| AATGAGGGAAGAGTGCTTGA+GGG | 0.489682 | 3.3:+65960149 | MS.gene29359:CDS |
| AGGTTCCAATGCAGATTTCG+TGG | 0.491662 | 3.3:+65957591 | MS.gene29359:intron |
| GAATGATCCTCCTACCAAAT+CGG | 0.494985 | 3.3:-65963095 | None:intergenic |
| TTGCTTGAGCTGAGCCAGAA+AGG | 0.495131 | 3.3:+65955824 | MS.gene29359:CDS |
| CATGGAAGCTTTCAACTAGC+AGG | 0.496010 | 3.3:-65963963 | None:intergenic |
| TAAAATGCGCAGACCTTGGC+GGG | 0.497032 | 3.3:+65961014 | MS.gene29359:CDS |
| TGCATACGGCAAGTTAGGCT+TGG | 0.498634 | 3.3:+65960113 | MS.gene29359:CDS |
| AGAGCTATCGACATGCTTGT+TGG | 0.498893 | 3.3:+65955854 | MS.gene29359:CDS |
| AGGGTTAGTGGGACAAATGT+TGG | 0.499572 | 3.3:+65955659 | MS.gene29359:CDS |
| CAACATTAACTAGAAGATAA+GGG | 0.500111 | 3.3:-65955487 | None:intergenic |
| TGTGTTGATCTCTATCATAA+AGG | 0.501529 | 3.3:+65960958 | MS.gene29359:CDS |
| TCTCCATCTAGTACCAACTT+TGG | 0.502815 | 3.3:+65957763 | MS.gene29359:CDS |
| AACGGAAACACTTCCAAAGT+TGG | 0.505796 | 3.3:-65957776 | None:intergenic |
| AAAGAGAGAATGTTAGGGAC+CGG | 0.506027 | 3.3:+65961159 | MS.gene29359:CDS |
| ATGGGGATGGGAAGTTTGAG+AGG | 0.506268 | 3.3:+65955639 | MS.gene29359:CDS |
| AGGGATGCTTCCTCTGGCTA+GGG | 0.513859 | 3.3:+65961251 | MS.gene29359:CDS |
| CAATACATAGATCCAGTTCA+AGG | 0.514523 | 3.3:+65952926 | MS.gene29359:CDS |
| TCCAATGCAGATTTCGTGGA+TGG | 0.514701 | 3.3:+65957595 | MS.gene29359:intron |
| GCAATAAAATGCGCAGACCT+TGG | 0.515821 | 3.3:+65961010 | MS.gene29359:CDS |
| GAACTGATGATGCCAAAAGA+AGG | 0.520146 | 3.3:+65959311 | MS.gene29359:CDS |
| TGGCCAATCTATTGGAAATG+AGG | 0.520415 | 3.3:+65960133 | MS.gene29359:CDS |
| CATCTCGCTCTTGATATTGG+AGG | 0.522421 | 3.3:+65953042 | MS.gene29359:CDS |
| ATGGAGGAGCCTTCTGCATA+CGG | 0.524279 | 3.3:+65960099 | MS.gene29359:CDS |
| GTCATTATTACCTTGACAAG+TGG | 0.524525 | 3.3:-65959363 | None:intergenic |
| TTCCGGTGTTAGTATGATCA+AGG | 0.525075 | 3.3:+65955511 | MS.gene29359:CDS |
| ACCAACAACATGGCTCCTAC+AGG | 0.525280 | 3.3:+65952898 | MS.gene29359:CDS |
| ACATATCACTCTCTCTTCTA+CGG | 0.529289 | 3.3:+65956153 | MS.gene29359:CDS |
| CCATGAAGCTTTCACACACA+TGG | 0.530882 | 3.3:+65955421 | MS.gene29359:CDS |
| AAGTCTTTCTTTGAAAAGGT+CGG | 0.531063 | 3.3:-65954772 | None:intergenic |
| GGCCAATCTATTGGAAATGA+GGG | 0.531273 | 3.3:+65960134 | MS.gene29359:CDS |
| ACATCAAATGAATAGGTTGA+TGG | 0.531786 | 3.3:+65960080 | MS.gene29359:intron |
| GTTAGTTGAAAGGTTTCCGA+TGG | 0.532451 | 3.3:+65956767 | MS.gene29359:CDS |
| ACAGTAAAGCTCTTCTATGA+GGG | 0.532783 | 3.3:-65961197 | None:intergenic |
| TCAAGTTCTACAAACAAGTC+AGG | 0.533102 | 3.3:-65960346 | None:intergenic |
| TAAAAGGAAGAAAACATTGA+AGG | 0.533223 | 3.3:+65953999 | MS.gene29359:CDS |
| AGCAAATGAAAGAGTGTCCA+TGG | 0.533459 | 3.3:-65956521 | None:intergenic |
| AAGATACATGGCCCACCACT+TGG | 0.534164 | 3.3:+65956810 | MS.gene29359:CDS |
| GCAACAGGAGTCATCGGCAC+AGG | 0.535483 | 3.3:-65957653 | None:intergenic |
| GCTTTCCTATTCTTGGTGGA+AGG | 0.535678 | 3.3:+65954055 | MS.gene29359:CDS |
| TATACTGGTGGCAAGATACA+TGG | 0.537423 | 3.3:+65956798 | MS.gene29359:CDS |
| GTGATGAAGGCTACTGGAGG+TGG | 0.539097 | 3.3:+65954735 | MS.gene29359:intron |
| TGTGGATAATAGGGAAAATG+AGG | 0.541454 | 3.3:+65960306 | MS.gene29359:intron |
| TGGTGCATCAGTTAGTTGAA+AGG | 0.545222 | 3.3:+65956757 | MS.gene29359:CDS |
| TATTGTAGCTGAGGCTGCCA+AGG | 0.545712 | 3.3:+65961950 | MS.gene29359:CDS |
| ATAAAATGCGCAGACCTTGG+CGG | 0.549032 | 3.3:+65961013 | MS.gene29359:CDS |
| AAATGAGGGAAGAGTGCTTG+AGG | 0.549228 | 3.3:+65960148 | MS.gene29359:CDS |
| TTATTTGTCATAGGTTGATG+GGG | 0.552120 | 3.3:+65955622 | MS.gene29359:intron |
| AGAAGCTTTCCTATTCTTGG+TGG | 0.558179 | 3.3:+65954051 | MS.gene29359:CDS |
| GGAAGTTTGAGAGGGTTAGT+GGG | 0.558531 | 3.3:+65955648 | MS.gene29359:CDS |
| AAGAGAGAATGTTAGGGACC+GGG | 0.558973 | 3.3:+65961160 | MS.gene29359:CDS |
| GGCAGGATCTCTGATAAAGT+TGG | 0.559378 | 3.3:+65953939 | MS.gene29359:intron |
| TCATTATTACCTTGACAAGT+GGG | 0.560771 | 3.3:-65959362 | None:intergenic |
| TCATTCAAATCGCCAAGTGG+TGG | 0.560907 | 3.3:-65956822 | None:intergenic |
| TAGGTCCAATGGAGAAGCCC+AGG | 0.563858 | 3.3:+65956653 | MS.gene29359:intron |
| CAGGTGCTGACATTGTTCTA+GGG | 0.564205 | 3.3:+65961232 | MS.gene29359:CDS |
| ACCTGTCTTAAGTCTATACA+TGG | 0.565218 | 3.3:-65963040 | None:intergenic |
| GATTTCATACAACATCGGTC+AGG | 0.567822 | 3.3:+65956178 | MS.gene29359:CDS |
| TGATTAGGCAACAAAATGGT+GGG | 0.574093 | 3.3:-65953003 | None:intergenic |
| GACAAATCATCAGATTGATT+AGG | 0.574243 | 3.3:-65953018 | None:intergenic |
| TTTGCTTACTCTAATTCACT+GGG | 0.574456 | 3.3:-65958283 | None:intergenic |
| CCATTCTCAACAACCATCAA+GGG | 0.574628 | 3.3:-65963007 | None:intergenic |
| CACCTTGATCATACTAACAC+CGG | 0.575151 | 3.3:-65955513 | None:intergenic |
| CAGGGGTCATGCTTATACCA+TGG | 0.575740 | 3.3:+65956504 | MS.gene29359:CDS |
| GCTTCCTGATATTGTAGCTG+AGG | 0.576697 | 3.3:+65961941 | MS.gene29359:CDS |
| GCAGTCACATCATTCAAAGC+AGG | 0.576932 | 3.3:-65961912 | None:intergenic |
| TTGATTAGGCAACAAAATGG+TGG | 0.577087 | 3.3:-65953004 | None:intergenic |
| CTATGTATTGGATTGCCTGT+AGG | 0.578163 | 3.3:-65952913 | None:intergenic |
| AGGCGAGCTGCTGAAGCTGG+AGG | 0.578900 | 3.3:+65962926 | MS.gene29359:CDS |
| CAGGCTGTTGCCAGTGAAGG+TGG | 0.580036 | 3.3:+65959289 | MS.gene29359:intron |
| CCTAACTTGCCGTATGCAGA+AGG | 0.581095 | 3.3:-65960108 | None:intergenic |
| AGAGCACTTACCAGATCTTG+TGG | 0.584881 | 3.3:+65958408 | MS.gene29359:CDS |
| CATTCAAATCGCCAAGTGGT+GGG | 0.589143 | 3.3:-65956821 | None:intergenic |
| ACTTGGCGATTTGAATGAGA+AGG | 0.589246 | 3.3:+65956827 | MS.gene29359:CDS |
| CTTGGTATTGACGAATCTGA+AGG | 0.589757 | 3.3:+65952862 | MS.gene29359:CDS |
| GACTGTTAACAAAGTGCAAG+AGG | 0.589787 | 3.3:+65955708 | MS.gene29359:CDS |
| AGAACTTGATAGTATGGATG+AGG | 0.590772 | 3.3:+65960360 | MS.gene29359:CDS |
| TTAATTCCACCTACCCGCCA+AGG | 0.591741 | 3.3:-65961027 | None:intergenic |
| GTTCCAGCAACAGGAGTCAT+CGG | 0.593671 | 3.3:-65957659 | None:intergenic |
| CTTGTTGGGGACATTTATGG+TGG | 0.594785 | 3.3:+65955869 | MS.gene29359:CDS |
| CAACAACAACACCAACAACA+TGG | 0.594798 | 3.3:+65952888 | MS.gene29359:CDS |
| TCTTCAAAGAGAGAATGTTA+GGG | 0.595349 | 3.3:+65961154 | MS.gene29359:CDS |
| CTTACCAGATCTTGTGGACA+AGG | 0.596291 | 3.3:+65958414 | MS.gene29359:CDS |
| ATAGAGATCAACACATGCAC+GGG | 0.596357 | 3.3:-65960950 | None:intergenic |
| GCTAGGGAGCTCCTCCGTCG+TGG | 0.596909 | 3.3:+65961267 | MS.gene29359:CDS |
| GAAGCTTTCAACTAGCAGGC+TGG | 0.597201 | 3.3:-65963959 | None:intergenic |
| GAAATGAATTGTCTTGTGGC+AGG | 0.597674 | 3.3:+65954819 | MS.gene29359:CDS |
| GTTGGTGGTGGTACTTATTG+GGG | 0.602827 | 3.3:+65955677 | MS.gene29359:CDS |
| TCCATGTATAGACTTAAGAC+AGG | 0.602929 | 3.3:+65963039 | MS.gene29359:CDS |
| GCATGGTTTAGATGACCTCA+TGG | 0.603918 | 3.3:+65956737 | MS.gene29359:CDS |
| TGGGGATGGGAAGTTTGAGA+GGG | 0.604314 | 3.3:+65955640 | MS.gene29359:CDS |
| AGTGGGACAAATGTTGGTGG+TGG | 0.605265 | 3.3:+65955665 | MS.gene29359:CDS |
| AGACTTAAGACAGGTCAGCT+CGG | 0.605465 | 3.3:+65963048 | MS.gene29359:CDS |
| TGAAATTCAATCTAATGTTG+AGG | 0.610246 | 3.3:+65952972 | MS.gene29359:CDS |
| ATGGTTGTTGAGAATGGGTG+CGG | 0.610452 | 3.3:+65963013 | MS.gene29359:CDS |
| AGCTATCGACATGCTTGTTG+GGG | 0.611783 | 3.3:+65955856 | MS.gene29359:CDS |
| GTTAGTGGGACAAATGTTGG+TGG | 0.614321 | 3.3:+65955662 | MS.gene29359:CDS |
| CACACTTAAACTGAGCATAG+AGG | 0.616056 | 3.3:-65963237 | None:intergenic |
| GCTGGAACTACTGGACTTGG+GGG | 0.616473 | 3.3:+65957674 | MS.gene29359:CDS |
| GATAGAGATCAACACATGCA+CGG | 0.617200 | 3.3:-65960951 | None:intergenic |
| TTAGTTGAAAGGTTTCCGAT+GGG | 0.620541 | 3.3:+65956768 | MS.gene29359:CDS |
| TTTGAATGATGTGACTGCAA+TGG | 0.622671 | 3.3:+65961917 | MS.gene29359:CDS |
| GATGCGAGTGCTCTAAATGT+TGG | 0.622823 | 3.3:+65957737 | MS.gene29359:CDS |
| AACTGATGATGCCAAAAGAA+GGG | 0.624687 | 3.3:+65959312 | MS.gene29359:CDS |
| AGAGAGAATGTTAGGGACCG+GGG | 0.628373 | 3.3:+65961161 | MS.gene29359:CDS |
| ATGGGTGCTCCATATACTGG+TGG | 0.639105 | 3.3:+65956786 | MS.gene29359:CDS |
| CCAGTATATGGAGCACCCAT+CGG | 0.641614 | 3.3:-65956783 | None:intergenic |
| TTTATGAGTTACGAAAAGCA+TGG | 0.645096 | 3.3:+65956720 | MS.gene29359:CDS |
| GCTTTGCTAACATCAAGTTG+AGG | 0.645930 | 3.3:-65952949 | None:intergenic |
| TCAAATGAATAGGTTGATGG+AGG | 0.647737 | 3.3:+65960083 | MS.gene29359:intron |
| GAAGCTTTCACACACATGGA+GGG | 0.648007 | 3.3:+65955425 | MS.gene29359:CDS |
| CCTATAGAAGTATAAAGCAG+AGG | 0.649593 | 3.3:+65960190 | MS.gene29359:CDS |
| CAGTAAAGCTCTTCTATGAG+GGG | 0.649740 | 3.3:-65961196 | None:intergenic |
| ATGCAGGCTGTTGCCAGTGA+AGG | 0.652213 | 3.3:+65959286 | MS.gene29359:intron |
| AGATGAAATGAATTGTCTTG+TGG | 0.660802 | 3.3:+65954815 | MS.gene29359:CDS |
| CGGAGGAGCTCCCTAGCCAG+AGG | 0.661956 | 3.3:-65961261 | None:intergenic |
| AGTAAAGCTCTTCTATGAGG+GGG | 0.666765 | 3.3:-65961195 | None:intergenic |
| CGGATGATTTCATACAACAT+CGG | 0.672578 | 3.3:+65956173 | MS.gene29359:CDS |
| TGAAGCTTTCACACACATGG+AGG | 0.674064 | 3.3:+65955424 | MS.gene29359:CDS |
| TCACTTCAGTTCCACGACGG+AGG | 0.674137 | 3.3:-65961278 | None:intergenic |
| TTCTCATTCAAATCGCCAAG+TGG | 0.675820 | 3.3:-65956825 | None:intergenic |
| CATTCTCAACAACCATCAAG+GGG | 0.678004 | 3.3:-65963006 | None:intergenic |
| ACTGATGATGCCAAAAGAAG+GGG | 0.688906 | 3.3:+65959313 | MS.gene29359:CDS |
| GTCACGTTACTCAGATACTG+AGG | 0.699781 | 3.3:+65954314 | MS.gene29359:CDS |
| AACTCACTTCAGTTCCACGA+CGG | 0.704303 | 3.3:-65961281 | None:intergenic |
| CAACTAACTGATGCACCATG+AGG | 0.704343 | 3.3:-65956752 | None:intergenic |
| TTTGTGATGAAGGCTACTGG+AGG | 0.733686 | 3.3:+65954732 | MS.gene29359:intron |
| CATTCAAAGCAGCTTCACCG+TGG | 0.733695 | 3.3:+65954132 | MS.gene29359:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 65952823 | 65963974 | 65952823 | ID=MS.gene29359 |
| chr3.3 | mRNA | 65952823 | 65963974 | 65952823 | ID=MS.gene29359.t1;Parent=MS.gene29359 |
| chr3.3 | exon | 65952823 | 65953063 | 65952823 | ID=MS.gene29359.t1.exon1;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65952823 | 65953063 | 65952823 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65953944 | 65954153 | 65953944 | ID=MS.gene29359.t1.exon2;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65953944 | 65954153 | 65953944 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65954307 | 65954359 | 65954307 | ID=MS.gene29359.t1.exon3;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65954307 | 65954359 | 65954307 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65954744 | 65954860 | 65954744 | ID=MS.gene29359.t1.exon4;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65954744 | 65954860 | 65954744 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65955413 | 65955532 | 65955413 | ID=MS.gene29359.t1.exon5;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65955413 | 65955532 | 65955413 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65955635 | 65955729 | 65955635 | ID=MS.gene29359.t1.exon6;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65955635 | 65955729 | 65955635 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65955814 | 65955907 | 65955814 | ID=MS.gene29359.t1.exon7;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65955814 | 65955907 | 65955814 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65956068 | 65956199 | 65956068 | ID=MS.gene29359.t1.exon8;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65956068 | 65956199 | 65956068 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65956442 | 65956554 | 65956442 | ID=MS.gene29359.t1.exon9;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65956442 | 65956554 | 65956442 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65956656 | 65956848 | 65956656 | ID=MS.gene29359.t1.exon10;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65956656 | 65956848 | 65956656 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65957605 | 65957816 | 65957605 | ID=MS.gene29359.t1.exon11;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65957605 | 65957816 | 65957605 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65958252 | 65958296 | 65958252 | ID=MS.gene29359.t1.exon12;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65958252 | 65958296 | 65958252 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65958387 | 65958435 | 65958387 | ID=MS.gene29359.t1.exon13;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65958387 | 65958435 | 65958387 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65959292 | 65959374 | 65959292 | ID=MS.gene29359.t1.exon14;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65959292 | 65959374 | 65959292 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65960095 | 65960211 | 65960095 | ID=MS.gene29359.t1.exon15;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65960095 | 65960211 | 65960095 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65960318 | 65960381 | 65960318 | ID=MS.gene29359.t1.exon16;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65960318 | 65960381 | 65960318 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65960889 | 65961035 | 65960889 | ID=MS.gene29359.t1.exon17;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65960889 | 65961035 | 65960889 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65961138 | 65961296 | 65961138 | ID=MS.gene29359.t1.exon18;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65961138 | 65961296 | 65961138 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65961888 | 65961971 | 65961888 | ID=MS.gene29359.t1.exon19;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65961888 | 65961971 | 65961888 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65962911 | 65963102 | 65962911 | ID=MS.gene29359.t1.exon20;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65962911 | 65963102 | 65962911 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65963199 | 65963270 | 65963199 | ID=MS.gene29359.t1.exon21;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65963199 | 65963270 | 65963199 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| chr3.3 | exon | 65963885 | 65963974 | 65963885 | ID=MS.gene29359.t1.exon22;Parent=MS.gene29359.t1 |
| chr3.3 | CDS | 65963885 | 65963974 | 65963885 | ID=cds.MS.gene29359.t1;Parent=MS.gene29359.t1 |
| Gene Sequence |
| Protein sequence |
