AlfalfaGEDB | MS.gene29820

M. sativa cultivar XinJiangDaYe / MS.gene29820

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005787 GO:0006465 GO:0006508 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009987 GO:0016021 GO:0016485 GO:0016787 GO:0019538 GO:0031224 GO:0032991 GO:0034641 GO:0043170 GO:0043603 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0051604 GO:0071704 GO:0098796 GO:0110165 GO:0140096 GO:1901564 GO:1902494 GO:1905368
PFAM: PF06645.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29820.t1	XP_024634987.1	95.3	85	4	0	1	85	1	85	2.70E-38	167.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29820.t1	Q7XI45	47.2	72	38	0	1	72	1	72	3.7e-12	72.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29820.t1	A0A072V0P5	97.6	82	2	0	1	82	1	82	7.5e-38	165.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049151	MS.gene29820	0.804689	1.92E-49	-1.69E-46
MS.gene049260	MS.gene29820	0.830812	2.38E-55	-1.69E-46
MS.gene049758	MS.gene29820	0.858293	9.10E-63	-1.69E-46
MS.gene049915	MS.gene29820	0.814207	1.73E-51	-1.69E-46
MS.gene050128	MS.gene29820	0.816835	4.51E-52	-1.69E-46
MS.gene050277	MS.gene29820	0.806188	9.29E-50	-1.69E-46
MS.gene050623	MS.gene29820	-0.80072	1.26E-48	-1.69E-46
MS.gene051662	MS.gene29820	0.835707	1.43E-56	-1.69E-46
MS.gene051663	MS.gene29820	0.816666	4.92E-52	-1.69E-46
MS.gene051789	MS.gene29820	0.804262	2.35E-49	-1.69E-46
MS.gene052178	MS.gene29820	0.83243	9.50E-56	-1.69E-46
MS.gene052286	MS.gene29820	0.80646	8.14E-50	-1.69E-46
MS.gene053074	MS.gene29820	0.805763	1.14E-49	-1.69E-46
MS.gene053630	MS.gene29820	0.815829	7.57E-52	-1.69E-46
MS.gene053836	MS.gene29820	0.825686	4.09E-54	-1.69E-46
MS.gene054184	MS.gene29820	0.818176	2.25E-52	-1.69E-46
MS.gene054185	MS.gene29820	0.823093	1.67E-53	-1.69E-46
MS.gene054942	MS.gene29820	0.802268	6.08E-49	-1.69E-46
MS.gene055133	MS.gene29820	0.877849	4.52E-69	-1.69E-46
MS.gene055207	MS.gene29820	0.804881	1.75E-49	-1.69E-46
MS.gene055422	MS.gene29820	0.830319	3.14E-55	-1.69E-46
MS.gene056069	MS.gene29820	-0.816826	4.53E-52	-1.69E-46
MS.gene057260	MS.gene29820	0.82972	4.40E-55	-1.69E-46
MS.gene057439	MS.gene29820	0.826883	2.12E-54	-1.69E-46
MS.gene057754	MS.gene29820	0.842962	1.88E-58	-1.69E-46
MS.gene058002	MS.gene29820	0.805413	1.35E-49	-1.69E-46
MS.gene058201	MS.gene29820	0.803821	2.90E-49	-1.69E-46
MS.gene058749	MS.gene29820	0.810602	1.06E-50	-1.69E-46
MS.gene058833	MS.gene29820	0.806217	9.16E-50	-1.69E-46
MS.gene058845	MS.gene29820	0.806548	7.80E-50	-1.69E-46
MS.gene059228	MS.gene29820	0.802039	6.78E-49	-1.69E-46
MS.gene059518	MS.gene29820	0.806016	1.01E-49	-1.69E-46
MS.gene059894	MS.gene29820	0.814034	1.89E-51	-1.69E-46
MS.gene059899	MS.gene29820	0.805535	1.27E-49	-1.69E-46
MS.gene060476	MS.gene29820	0.867556	1.25E-65	-1.69E-46
MS.gene061113	MS.gene29820	0.849269	3.60E-60	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29820.t1	MTR_3g074500	96.386	83	3	0	1	83	1	83	5.57e-55	166
MS.gene29820.t1	MTR_3g074500	96.386	83	3	0	1	83	1	83	8.53e-55	166

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29820.t1	AT1G34640	58.537	82	32	1	1	80	1	82	9.22e-23	84.7

Find 18 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGGTATTGTTGGAATTTC+TGG	0.095978	3.4:-72993657	MS.gene29820:CDS
GATCTTTCCTCAACAGTAAC+AGG	0.350655	3.4:+72993579	None:intergenic
TTGGCTGGCAGCAGTTATAT+TGG	0.382907	3.4:-72993730	MS.gene29820:CDS
ACCTATGTGTTGGGTATTGT+TGG	0.449040	3.4:-72993666	MS.gene29820:CDS
TCCTCAACAGTAACAGGATA+AGG	0.457912	3.4:+72993585	None:intergenic
ATGTTCGTTACCTATGTGTT+GGG	0.458889	3.4:-72993675	MS.gene29820:CDS
CGGTTGAAATAATCCCAATC+AGG	0.486481	3.4:+72993624	None:intergenic
TCAGCAGCTTCTCTTTCTCA+TGG	0.490405	3.4:-72993558	MS.gene29820:CDS
CTCTAAGAACCTCTATACTT+TGG	0.491621	3.4:-72993749	MS.gene29820:CDS
GCCTTATCCTGTTACTGTTG+AGG	0.496290	3.4:-72993586	MS.gene29820:CDS
GCAGCAGTTATATTGGTAGT+TGG	0.498983	3.4:-72993723	MS.gene29820:CDS
AAGAACCTCTATACTTTGGC+TGG	0.505897	3.4:-72993745	MS.gene29820:CDS
GATGTTCGTTACCTATGTGT+TGG	0.512795	3.4:-72993676	MS.gene29820:CDS
TAGAGCTGCATCATTTGCCA+TGG	0.517008	3.4:+72993767	None:intergenic
TGCTGCCAGCCAAAGTATAG+AGG	0.518224	3.4:+72993740	None:intergenic
TCAACCGTGATATTTCTCGT+TGG	0.602906	3.4:-72993608	MS.gene29820:CDS
TCCAACAATACCCAACACAT+AGG	0.663389	3.4:+72993665	None:intergenic
AAGGCCAACGAGAAATATCA+CGG	0.673257	3.4:+72993604	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAGAATATAAATGAATTAA+GGG	+	chr3.4:72993727-72993746	None:intergenic	10.0%
!!	TAAAGAATATAAATGAATTA+AGG	+	chr3.4:72993728-72993747	None:intergenic	10.0%
	ATGTTCGTTACCTATGTGTT+GGG	-	chr3.4:72993563-72993582	MS.gene29820:CDS	35.0%
!	CTCTAAGAACCTCTATACTT+TGG	-	chr3.4:72993489-72993508	MS.gene29820:intron	35.0%
!	TTGGGTATTGTTGGAATTTC+TGG	-	chr3.4:72993581-72993600	MS.gene29820:CDS	35.0%
	AAGAACCTCTATACTTTGGC+TGG	-	chr3.4:72993493-72993512	MS.gene29820:intron	40.0%
	AAGGCCAACGAGAAATATCA+CGG	+	chr3.4:72993637-72993656	None:intergenic	40.0%
	CGGTTGAAATAATCCCAATC+AGG	+	chr3.4:72993617-72993636	None:intergenic	40.0%
	GATCTTTCCTCAACAGTAAC+AGG	+	chr3.4:72993662-72993681	None:intergenic	40.0%
	GATGTTCGTTACCTATGTGT+TGG	-	chr3.4:72993562-72993581	MS.gene29820:CDS	40.0%
	TCAACCGTGATATTTCTCGT+TGG	-	chr3.4:72993630-72993649	MS.gene29820:CDS	40.0%
	TCCAACAATACCCAACACAT+AGG	+	chr3.4:72993576-72993595	None:intergenic	40.0%
	TCCTCAACAGTAACAGGATA+AGG	+	chr3.4:72993656-72993675	None:intergenic	40.0%
!	ACCTATGTGTTGGGTATTGT+TGG	-	chr3.4:72993572-72993591	MS.gene29820:CDS	40.0%
!	GCAGCAGTTATATTGGTAGT+TGG	-	chr3.4:72993515-72993534	MS.gene29820:intron	40.0%
!	TGGTGTTCTTTTGCCTGATT+GGG	-	chr3.4:72993601-72993620	MS.gene29820:CDS	40.0%
	GCCTTATCCTGTTACTGTTG+AGG	-	chr3.4:72993652-72993671	MS.gene29820:CDS	45.0%
	TCAGCAGCTTCTCTTTCTCA+TGG	-	chr3.4:72993680-72993699	MS.gene29820:CDS	45.0%
	TTGGCTGGCAGCAGTTATAT+TGG	-	chr3.4:72993508-72993527	MS.gene29820:intron	45.0%
!	CTGGTGTTCTTTTGCCTGAT+TGG	-	chr3.4:72993600-72993619	MS.gene29820:CDS	45.0%
	TGCTGCCAGCCAAAGTATAG+AGG	+	chr3.4:72993501-72993520	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	72993473	72993787	72993473	ID=MS.gene29820
chr3.4	mRNA	72993473	72993787	72993473	ID=MS.gene29820.t1;Parent=MS.gene29820
chr3.4	exon	72993543	72993787	72993543	ID=MS.gene29820.t1.exon1;Parent=MS.gene29820.t1
chr3.4	CDS	72993543	72993787	72993543	ID=cds.MS.gene29820.t1;Parent=MS.gene29820.t1
chr3.4	exon	72993473	72993497	72993473	ID=MS.gene29820.t1.exon2;Parent=MS.gene29820.t1
chr3.4	CDS	72993473	72993497	72993473	ID=cds.MS.gene29820.t1;Parent=MS.gene29820.t1

Gene Sequence	>MS.gene29820 ATGGCAAATGATGCAGCTCTAAGAACCTCTATACTTTGGCTGGCAGCAGTTATATTGGTAGTTGGTATATGCACATACTCTTTCAAGAAGATGTTCGTTACCTATGTGTTGGGTATTGTTGGAATTTCTGGTGTTCTTTTGCCTGATTGGGATTATTTCAACCGTGATATTTCTCGTTGGCCTTATCCTGTTACTGTTGAGGAAAGATCAGCAGCTTCTCTTTCTCATGGATCTGTATTTCTAAAGTGAGTCCCTTAATTCATTTATATTCTTTATATATACTCTTGTAGACTAGATTTTGATGAATCAATTTAA
Protein sequence	>MS.gene29820.t1 MANDAALRTSILWLAAVILVVGICTYSFKKMFVTYVLGIVGISGVLLPDWDYFNRDISRWPYPVTVEERSAASLSHGSVFLKLDFDESI