AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30928

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30928.t1 MTR_2g096210 96.795 468 13 2 1 466 13 480 0.0 936
MS.gene30928.t1 MTR_4g062270 71.335 457 120 3 1 456 13 459 0.0 707
MS.gene30928.t1 MTR_4g062170 72.570 463 122 3 1 462 13 471 0.0 703
MS.gene30928.t1 MTR_4g062190 70.259 464 132 3 1 462 13 472 0.0 686
MS.gene30928.t1 MTR_1g115560 54.566 449 184 5 13 447 26 468 0.0 516
MS.gene30928.t1 MTR_7g118170 44.186 430 221 6 14 436 117 534 1.95e-123 370
MS.gene30928.t1 MTR_1g076430 41.379 435 233 8 3 432 88 505 2.61e-109 333
MS.gene30928.t1 MTR_1g110930 40.553 434 238 7 3 432 72 489 3.77e-109 332
MS.gene30928.t1 MTR_3g105550 39.813 427 234 5 15 430 119 533 5.25e-109 333
MS.gene30928.t1 MTR_5g056510 41.055 436 234 7 4 432 70 489 6.97e-109 332
MS.gene30928.t1 MTR_4g070270 40.000 420 232 5 15 430 96 499 6.86e-108 329
MS.gene30928.t1 MTR_2g054940 39.906 426 225 6 15 430 98 502 9.86e-106 324
MS.gene30928.t1 MTR_1g041085 39.007 423 237 4 15 432 105 511 1.96e-104 321
MS.gene30928.t1 MTR_7g118160 43.443 366 179 6 79 432 69 418 1.07e-101 311
MS.gene30928.t1 MTR_4g054415 37.793 426 243 5 11 430 13 422 1.05e-98 303
MS.gene30928.t1 MTR_4g054630 37.793 426 243 5 11 430 54 463 2.32e-98 303
MS.gene30928.t1 MTR_4g113280 36.986 438 224 6 15 448 87 476 6.06e-97 300
MS.gene30928.t1 MTR_2g007020 39.808 417 237 4 15 430 45 448 4.99e-96 297
MS.gene30928.t1 MTR_7g081695 38.425 419 225 5 17 430 100 490 6.54e-95 303
MS.gene30928.t1 MTR_6g074880 38.955 421 227 10 20 430 65 465 3.16e-94 293
MS.gene30928.t1 MTR_2g436480 36.967 422 251 5 11 430 40 448 1.88e-91 285
MS.gene30928.t1 MTR_6g032965 39.378 386 211 5 52 430 12 381 6.74e-90 279
MS.gene30928.t1 MTR_2g013740 35.784 408 243 4 17 422 110 500 6.52e-88 278
MS.gene30928.t1 MTR_1g102290 37.647 425 237 8 15 430 78 483 1.87e-85 271
MS.gene30928.t1 MTR_3g072940 37.055 421 234 8 20 430 83 482 5.64e-82 261
MS.gene30928.t1 MTR_3g072980 37.055 421 234 8 20 430 83 482 6.42e-82 261
MS.gene30928.t1 MTR_6g051440 37.352 423 230 8 20 430 82 481 3.46e-77 249
MS.gene30928.t1 MTR_4g054260 38.806 268 126 5 169 430 87 322 4.96e-54 184
MS.gene30928.t1 MTR_4g053905 37.870 169 85 3 267 430 3 156 3.05e-32 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30928.t1 AT2G28630 66.300 454 148 3 7 456 19 471 0.0 636
MS.gene30928.t1 AT2G28630 66.300 454 148 3 7 456 19 471 0.0 636
MS.gene30928.t1 AT1G07720 65.310 467 143 4 2 456 57 516 0.0 631
MS.gene30928.t1 AT1G07720 65.310 467 143 4 2 456 14 473 0.0 630
MS.gene30928.t1 AT5G04530 53.244 447 196 5 5 447 18 455 2.28e-179 510
MS.gene30928.t1 AT1G01120 43.192 426 212 6 15 430 118 523 4.56e-123 369
MS.gene30928.t1 AT2G46720 38.940 434 248 6 3 432 44 464 9.83e-112 338
MS.gene30928.t1 AT4G34510 41.080 426 228 5 11 430 74 482 1.96e-110 335
MS.gene30928.t1 AT1G25450 41.435 432 233 7 3 430 68 483 3.25e-109 332
MS.gene30928.t1 AT4G34520 39.723 433 226 6 15 430 73 487 3.58e-108 330
MS.gene30928.t1 AT2G15090 38.182 440 248 7 1 430 51 476 3.27e-107 327
MS.gene30928.t1 AT2G16280 40.000 425 225 6 15 430 104 507 6.53e-107 327
MS.gene30928.t1 AT1G19440 38.353 425 232 6 15 430 108 511 3.46e-106 325
MS.gene30928.t1 AT5G43760 40.749 427 226 7 15 430 107 517 2.43e-105 323
MS.gene30928.t1 AT4G34250 38.426 432 228 6 15 430 75 484 3.17e-105 322
MS.gene30928.t1 AT2G26640 40.411 438 233 8 1 430 83 500 4.04e-105 322
MS.gene30928.t1 AT3G10280 37.182 433 250 5 3 432 44 457 5.19e-105 320
MS.gene30928.t1 AT1G68530 40.573 419 231 7 15 430 85 488 1.10e-104 321
MS.gene30928.t1 AT1G04220 38.864 440 240 8 3 430 90 512 2.54e-103 318
MS.gene30928.t1 AT2G26250 35.780 436 255 5 15 430 117 547 2.26e-98 306
MS.gene30928.t1 AT5G49070 37.176 425 255 4 6 430 40 452 6.12e-95 295
MS.gene30928.t1 AT1G71160 36.740 411 247 3 20 430 48 445 6.14e-92 286
MS.gene30928.t1 AT3G52160 31.796 412 236 4 4 414 73 440 3.15e-75 243
MS.gene30928.t1 AT3G52160 34.270 356 189 4 60 414 16 327 2.75e-71 229
MS.gene30928.t1 AT1G68530 38.007 271 161 3 15 282 85 351 5.80e-58 196
MS.gene30928.t1 AT5G49070 31.183 279 189 2 3 281 37 312 1.87e-40 147

Find 75 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAATGCAACAGTTGTTAT+TGG 0.230294 2.2:+5743083 MS.gene30928:CDS
CTTAGTACAAATTGGTATTC+AGG 0.248106 2.2:+5742437 MS.gene30928:CDS
AAAGCTCTTGTAGCTGCTTT+TGG 0.259427 2.2:-5742674 None:intergenic
TACCAGCTAGGGAAATATTT+AGG 0.265669 2.2:+5742732 MS.gene30928:CDS
ATGACATTCTTGCTGGTTCT+AGG 0.278483 2.2:-5742943 None:intergenic
CGGCCTTCGATGAAGTTTCT+TGG 0.294675 2.2:-5742101 None:intergenic
GTGGTATGTGTTGGGGTATA+TGG 0.299234 2.2:+5743000 MS.gene30928:CDS
GAACAATGTGGCAAGATTAT+AGG 0.312551 2.2:+5741993 MS.gene30928:CDS
TTACTATCCACCACACTCTT+TGG 0.331528 2.2:+5743162 MS.gene30928:CDS
CCATCGTTGGGTTCACAGTT+CGG 0.347303 2.2:-5742121 None:intergenic
TCCACTTCGTCTTCCATCGT+TGG 0.351430 2.2:-5742134 None:intergenic
CAGAGTCTCTTAGTACAAAT+TGG 0.351617 2.2:+5742429 MS.gene30928:CDS
ATAAAAGCTTATAACCTATC+TGG 0.357029 2.2:+5742317 MS.gene30928:CDS
AGAATGTCATTACATAGATT+TGG 0.358478 2.2:+5742956 MS.gene30928:CDS
TGCACTTGTGTTTCCTGATC+TGG 0.359947 2.2:+5741883 MS.gene30928:CDS
TAAATGCAACAGTTGTTATT+GGG 0.360260 2.2:+5743084 MS.gene30928:CDS
AGTTTGAGTTTGAAAGTGTT+TGG 0.363208 2.2:-5743232 None:intergenic
CTCTTTGGCCAACCCATTCA+TGG 0.371696 2.2:+5743177 MS.gene30928:CDS
CTATGTAATGACATTCTTGC+TGG 0.374650 2.2:-5742950 None:intergenic
AAAGCAGTGATAGATGGAAT+TGG 0.380405 2.2:+5742893 MS.gene30928:CDS
CCACTTCGTCTTCCATCGTT+GGG 0.388821 2.2:-5742133 None:intergenic
CAACCGTACTTCTCCATGAA+TGG 0.401060 2.2:-5743190 None:intergenic
GGAAGACGAAGTGGAGGAAA+TGG 0.412780 2.2:+5742142 MS.gene30928:CDS
CCAAGTGATGAAAGAAAACT+TGG 0.421127 2.2:+5741966 MS.gene30928:CDS
AGCTTATAACCTATCTGGTA+TGG 0.425685 2.2:+5742322 MS.gene30928:CDS
GCAAAGCTTCTAAACAAATC+AGG 0.429501 2.2:+5742188 MS.gene30928:CDS
ATAGGAAACAATGAGAATAT+TGG 0.429933 2.2:+5742011 MS.gene30928:CDS
CTGAGTTGACAACGGCTTTG+AGG 0.431728 2.2:-5742055 None:intergenic
ATTGGTATTCAGGAAACAAT+AGG 0.432471 2.2:+5742447 MS.gene30928:CDS
AAGCAGTGATAGATGGAATT+GGG 0.433566 2.2:+5742894 MS.gene30928:CDS
AAGTCAGCTGCAAGATGGTT+TGG 0.438277 2.2:+5742803 MS.gene30928:CDS
GGAGGAAAAGCAGTGATAGA+TGG 0.443045 2.2:+5742887 MS.gene30928:CDS
AGAGTATTGATGATAAGTTT+AGG 0.443493 2.2:+5743052 MS.gene30928:CDS
ATGATTCTTGAAGTTAAAGA+AGG 0.444901 2.2:-5742269 None:intergenic
TACCTAAATATTTCCCTAGC+TGG 0.449686 2.2:-5742734 None:intergenic
AGTAGTTTGTGGTATGTGTT+GGG 0.450299 2.2:+5742992 MS.gene30928:CDS
CAAATCAGGCATTTCACCTT+CGG 0.453132 2.2:+5742202 MS.gene30928:CDS
TCACCAGTGAATTTCAAAAC+AGG 0.477090 2.2:+5742839 MS.gene30928:CDS
CTCAAAGCCGTTGTCAACTC+AGG 0.488420 2.2:+5742056 MS.gene30928:CDS
AACCGTACTTCTCCATGAAT+GGG 0.497250 2.2:-5743189 None:intergenic
GAAGATGAACAAGGTAAACT+TGG 0.499726 2.2:+5742629 MS.gene30928:CDS
AACCATCTTGCAGCTGACTT+AGG 0.502261 2.2:-5742800 None:intergenic
AGTTTAATATGTAACACTCT+TGG 0.508115 2.2:-5741929 None:intergenic
CTCCTAAGTCAGCTGCAAGA+TGG 0.509644 2.2:+5742798 MS.gene30928:CDS
TTGATGATAAGTTTAGGTGC+TGG 0.511949 2.2:+5743058 MS.gene30928:CDS
AAGTAGTTTGTGGTATGTGT+TGG 0.515984 2.2:+5742991 MS.gene30928:CDS
GCCGTTGTCAACTCAGGCAT+TGG 0.520687 2.2:+5742062 MS.gene30928:CDS
GAAGTAATGAGAGATGTTGT+TGG 0.523138 2.2:+5743106 MS.gene30928:CDS
CACTACAACCCATACCAGAT+AGG 0.541455 2.2:-5742331 None:intergenic
AGAGATGTTGTTGGTGACGA+TGG 0.546076 2.2:+5743115 MS.gene30928:CDS
GCTTATAACCTATCTGGTAT+GGG 0.546638 2.2:+5742323 MS.gene30928:CDS
ATACATCAGCAAGTAGTTTG+TGG 0.556511 2.2:+5742981 MS.gene30928:CDS
GAATTGCACAACCGCCGGTA+CGG 0.562704 2.2:-5742496 None:intergenic
TTCGGAGATCGATATTCTTG+TGG 0.562977 2.2:+5742220 MS.gene30928:CDS
CATTCATGGAGAAGTACGGT+TGG 0.595870 2.2:+5743191 MS.gene30928:CDS
TGTCTAGTTAGAACTCATCA+TGG 0.608151 2.2:+5742569 MS.gene30928:CDS
AACCCATTCATGGAGAAGTA+CGG 0.611169 2.2:+5743187 MS.gene30928:CDS
GTACTTCTCCATGAATGGGT+TGG 0.611489 2.2:-5743185 None:intergenic
AACGATGGAAGACGAAGTGG+AGG 0.619961 2.2:+5742136 MS.gene30928:CDS
GGTATATGGAAGCTAAGAAG+AGG 0.621077 2.2:+5743014 MS.gene30928:CDS
TACGGAAAAGACAATTTGCA+AGG 0.623230 2.2:-5742478 None:intergenic
CCCAACGATGGAAGACGAAG+TGG 0.624451 2.2:+5742133 MS.gene30928:CDS
GCTCCAAGAAACTTCATCGA+AGG 0.628524 2.2:+5742098 MS.gene30928:CDS
GAGAAGAATTGCACAACCGC+CGG 0.629617 2.2:-5742501 None:intergenic
GTAGTTTGTGGTATGTGTTG+GGG 0.633510 2.2:+5742993 MS.gene30928:CDS
TAACCATTACAAAATGAGAG+AGG 0.653134 2.2:+5742292 MS.gene30928:CDS
TGTCAACTCAGGCATTGGTG+AGG 0.663337 2.2:+5742067 MS.gene30928:CDS
CCGAACTGTGAACCCAACGA+TGG 0.667415 2.2:+5742121 MS.gene30928:CDS
ACAAGAATATCGATCTCCGA+AGG 0.671733 2.2:-5742218 None:intergenic
TGGTGACGATGGAAATGTGT+GGG 0.681456 2.2:+5743126 MS.gene30928:CDS
ACCAATGCCTGAGTTGACAA+CGG 0.693257 2.2:-5742063 None:intergenic
AATGGGTTGGCCAAAGAGTG+TGG 0.696430 2.2:-5743172 None:intergenic
TTGGTGACGATGGAAATGTG+TGG 0.725183 2.2:+5743125 MS.gene30928:CDS
GGGTTGGCCAAAGAGTGTGG+TGG 0.735704 2.2:-5743169 None:intergenic
AAACTTGGAAGTGAACAATG+TGG 0.743662 2.2:+5741981 MS.gene30928:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGCTTTTACTGATAATTTAA+GGG + chr2.2:5742691-5742710 MS.gene30928:CDS 20.0%
! AGAATGTCATTACATAGATT+TGG + chr2.2:5742956-5742975 MS.gene30928:CDS 25.0%
! AGTTTAATATGTAACACTCT+TGG - chr2.2:5741932-5741951 None:intergenic 25.0%
! ATAAAAGCTTATAACCTATC+TGG + chr2.2:5742317-5742336 MS.gene30928:CDS 25.0%
! ATAGGAAACAATGAGAATAT+TGG + chr2.2:5742011-5742030 MS.gene30928:CDS 25.0%
! ATGATTCTTGAAGTTAAAGA+AGG - chr2.2:5742272-5742291 None:intergenic 25.0%
! GAAAAAATTTGATCAGAGAA+GGG + chr2.2:5741905-5741924 MS.gene30928:CDS 25.0%
! TTAAATGCAACAGTTGTTAT+TGG + chr2.2:5743083-5743102 MS.gene30928:CDS 25.0%
!! GAGCTTTTACTGATAATTTA+AGG + chr2.2:5742690-5742709 MS.gene30928:CDS 25.0%
!! TAAATGCAACAGTTGTTATT+GGG + chr2.2:5743084-5743103 MS.gene30928:CDS 25.0%
!!! AGAGTATTGATGATAAGTTT+AGG + chr2.2:5743052-5743071 MS.gene30928:CDS 25.0%
AGTCAAAAAGAAGATGAACA+AGG + chr2.2:5742620-5742639 MS.gene30928:CDS 30.0%
ATTGGTATTCAGGAAACAAT+AGG + chr2.2:5742447-5742466 MS.gene30928:CDS 30.0%
CTTAGTACAAATTGGTATTC+AGG + chr2.2:5742437-5742456 MS.gene30928:CDS 30.0%
GGAAAAAATTTGATCAGAGA+AGG + chr2.2:5741904-5741923 MS.gene30928:CDS 30.0%
TAACCATTACAAAATGAGAG+AGG + chr2.2:5742292-5742311 MS.gene30928:CDS 30.0%
! ACCTAAAATTTTACCAGCTA+GGG + chr2.2:5742721-5742740 MS.gene30928:CDS 30.0%
! TATCCTCTCTCATTTTGTAA+TGG - chr2.2:5742298-5742317 None:intergenic 30.0%
!! AGTTTGAGTTTGAAAGTGTT+TGG - chr2.2:5743235-5743254 None:intergenic 30.0%
!!! CAGTATAGCTTTGTGTTTTA+AGG - chr2.2:5742540-5742559 None:intergenic 30.0%
!!! GATCAAATTTTTTCCAGATC+AGG - chr2.2:5741899-5741918 None:intergenic 30.0%
AAACTTGGAAGTGAACAATG+TGG + chr2.2:5741981-5742000 MS.gene30928:CDS 35.0%
AAAGCAGTGATAGATGGAAT+TGG + chr2.2:5742893-5742912 MS.gene30928:CDS 35.0%
AAGCAGTGATAGATGGAATT+GGG + chr2.2:5742894-5742913 MS.gene30928:CDS 35.0%
AGCTTATAACCTATCTGGTA+TGG + chr2.2:5742322-5742341 MS.gene30928:CDS 35.0%
ATACATCAGCAAGTAGTTTG+TGG + chr2.2:5742981-5743000 MS.gene30928:CDS 35.0%
CAGAGTCTCTTAGTACAAAT+TGG + chr2.2:5742429-5742448 MS.gene30928:CDS 35.0%
CCAAGTGATGAAAGAAAACT+TGG + chr2.2:5741966-5741985 MS.gene30928:CDS 35.0%
CTATGTAATGACATTCTTGC+TGG - chr2.2:5742953-5742972 None:intergenic 35.0%
GAACAATGTGGCAAGATTAT+AGG + chr2.2:5741993-5742012 MS.gene30928:CDS 35.0%
GAAGATGAACAAGGTAAACT+TGG + chr2.2:5742629-5742648 MS.gene30928:CDS 35.0%
GAAGTAATGAGAGATGTTGT+TGG + chr2.2:5743106-5743125 MS.gene30928:CDS 35.0%
GCAAAGCTTCTAAACAAATC+AGG + chr2.2:5742188-5742207 MS.gene30928:CDS 35.0%
GCTAAGAAGAGGTTGAAAAA+AGG + chr2.2:5743025-5743044 MS.gene30928:CDS 35.0%
GCTTATAACCTATCTGGTAT+GGG + chr2.2:5742323-5742342 MS.gene30928:CDS 35.0%
TACCAGCTAGGGAAATATTT+AGG + chr2.2:5742732-5742751 MS.gene30928:CDS 35.0%
TACCTAAATATTTCCCTAGC+TGG - chr2.2:5742737-5742756 None:intergenic 35.0%
TACGGAAAAGACAATTTGCA+AGG - chr2.2:5742481-5742500 None:intergenic 35.0%
TCACCAGTGAATTTCAAAAC+AGG + chr2.2:5742839-5742858 MS.gene30928:CDS 35.0%
TGTCTAGTTAGAACTCATCA+TGG + chr2.2:5742569-5742588 MS.gene30928:CDS 35.0%
! AAGTAGTTTGTGGTATGTGT+TGG + chr2.2:5742991-5743010 MS.gene30928:CDS 35.0%
! AGTAGTTTGTGGTATGTGTT+GGG + chr2.2:5742992-5743011 MS.gene30928:CDS 35.0%
! CACCTAAAATTTTACCAGCT+AGG + chr2.2:5742720-5742739 MS.gene30928:CDS 35.0%
! CCAAGTTTTCTTTCATCACT+TGG - chr2.2:5741969-5741988 None:intergenic 35.0%
! GATCATTTTGTTCTTCACAC+TGG + chr2.2:5742866-5742885 MS.gene30928:CDS 35.0%
! TCCCTAGCTGGTAAAATTTT+AGG - chr2.2:5742725-5742744 None:intergenic 35.0%
! TTGATGATAAGTTTAGGTGC+TGG + chr2.2:5743058-5743077 MS.gene30928:CDS 35.0%
!! CTTTTGGAAGATCTTTAGCT+AGG - chr2.2:5742661-5742680 None:intergenic 35.0%
!!! ACACCTGTTTTGAAATTCAC+TGG - chr2.2:5742845-5742864 None:intergenic 35.0%
AACCCATTCATGGAGAAGTA+CGG + chr2.2:5743187-5743206 MS.gene30928:CDS 40.0%
AACCGTACTTCTCCATGAAT+GGG - chr2.2:5743192-5743211 None:intergenic 40.0%
ACAAGAATATCGATCTCCGA+AGG - chr2.2:5742221-5742240 None:intergenic 40.0%
CAAATCAGGCATTTCACCTT+CGG + chr2.2:5742202-5742221 MS.gene30928:CDS 40.0%
GGTATATGGAAGCTAAGAAG+AGG + chr2.2:5743014-5743033 MS.gene30928:CDS 40.0%
TTACTATCCACCACACTCTT+TGG + chr2.2:5743162-5743181 MS.gene30928:CDS 40.0%
TTCGGAGATCGATATTCTTG+TGG + chr2.2:5742220-5742239 MS.gene30928:CDS 40.0%
! CATTTTGTTCTTCACACTGG+AGG + chr2.2:5742869-5742888 MS.gene30928:CDS 40.0%
! GCAAATTGTCTTTTCCGTAC+CGG + chr2.2:5742482-5742501 MS.gene30928:CDS 40.0%
! GTAGTTTGTGGTATGTGTTG+GGG + chr2.2:5742993-5743012 MS.gene30928:CDS 40.0%
!! AAAGCTCTTGTAGCTGCTTT+TGG - chr2.2:5742677-5742696 None:intergenic 40.0%
!! ATGACATTCTTGCTGGTTCT+AGG - chr2.2:5742946-5742965 None:intergenic 40.0%
AAGTCAGCTGCAAGATGGTT+TGG + chr2.2:5742803-5742822 MS.gene30928:CDS 45.0%
CAACCGTACTTCTCCATGAA+TGG - chr2.2:5743193-5743212 None:intergenic 45.0%
CACTACAACCCATACCAGAT+AGG - chr2.2:5742334-5742353 None:intergenic 45.0%
GCTCCAAGAAACTTCATCGA+AGG + chr2.2:5742098-5742117 MS.gene30928:CDS 45.0%
GGAGGAAAAGCAGTGATAGA+TGG + chr2.2:5742887-5742906 MS.gene30928:CDS 45.0%
GTACTTCTCCATGAATGGGT+TGG - chr2.2:5743188-5743207 None:intergenic 45.0%
GTGGTATGTGTTGGGGTATA+TGG + chr2.2:5743000-5743019 MS.gene30928:CDS 45.0%
TGCACTTGTGTTTCCTGATC+TGG + chr2.2:5741883-5741902 MS.gene30928:CDS 45.0%
! AACCATCTTGCAGCTGACTT+AGG - chr2.2:5742803-5742822 None:intergenic 45.0%
! AATTGTCTTTTCCGTACCGG+CGG + chr2.2:5742485-5742504 MS.gene30928:CDS 45.0%
! ACCAATGCCTGAGTTGACAA+CGG - chr2.2:5742066-5742085 None:intergenic 45.0%
! CATTCATGGAGAAGTACGGT+TGG + chr2.2:5743191-5743210 MS.gene30928:CDS 45.0%
! TGGTGACGATGGAAATGTGT+GGG + chr2.2:5743126-5743145 MS.gene30928:CDS 45.0%
!! AGAGATGTTGTTGGTGACGA+TGG + chr2.2:5743115-5743134 MS.gene30928:CDS 45.0%
!! TTGGTGACGATGGAAATGTG+TGG + chr2.2:5743125-5743144 MS.gene30928:CDS 45.0%
AACGATGGAAGACGAAGTGG+AGG + chr2.2:5742136-5742155 MS.gene30928:CDS 50.0%
CCACTTCGTCTTCCATCGTT+GGG - chr2.2:5742136-5742155 None:intergenic 50.0%
CCATCGTTGGGTTCACAGTT+CGG - chr2.2:5742124-5742143 None:intergenic 50.0%
CGGCCTTCGATGAAGTTTCT+TGG - chr2.2:5742104-5742123 None:intergenic 50.0%
CTCAAAGCCGTTGTCAACTC+AGG + chr2.2:5742056-5742075 MS.gene30928:CDS 50.0%
CTCCTAAGTCAGCTGCAAGA+TGG + chr2.2:5742798-5742817 MS.gene30928:CDS 50.0%
CTCTTTGGCCAACCCATTCA+TGG + chr2.2:5743177-5743196 MS.gene30928:CDS 50.0%
GAGAAGAATTGCACAACCGC+CGG - chr2.2:5742504-5742523 None:intergenic 50.0%
GGAAGACGAAGTGGAGGAAA+TGG + chr2.2:5742142-5742161 MS.gene30928:CDS 50.0%
TCCACTTCGTCTTCCATCGT+TGG - chr2.2:5742137-5742156 None:intergenic 50.0%
! CTGAGTTGACAACGGCTTTG+AGG - chr2.2:5742058-5742077 None:intergenic 50.0%
!! AATGGGTTGGCCAAAGAGTG+TGG - chr2.2:5743175-5743194 None:intergenic 50.0%
!! TGTCAACTCAGGCATTGGTG+AGG + chr2.2:5742067-5742086 MS.gene30928:CDS 50.0%
CCCAACGATGGAAGACGAAG+TGG + chr2.2:5742133-5742152 MS.gene30928:CDS 55.0%
CCGAACTGTGAACCCAACGA+TGG + chr2.2:5742121-5742140 MS.gene30928:CDS 55.0%
GAATTGCACAACCGCCGGTA+CGG - chr2.2:5742499-5742518 None:intergenic 55.0%
GCCGTTGTCAACTCAGGCAT+TGG + chr2.2:5742062-5742081 MS.gene30928:CDS 55.0%
!! GGGTTGGCCAAAGAGTGTGG+TGG - chr2.2:5743172-5743191 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 5741882 5743282 5741882 ID=MS.gene30928
chr2.2 mRNA 5741882 5743282 5741882 ID=MS.gene30928.t1;Parent=MS.gene30928
chr2.2 exon 5741882 5743282 5741882 ID=MS.gene30928.t1.exon1;Parent=MS.gene30928.t1
chr2.2 CDS 5741882 5743282 5741882 ID=cds.MS.gene30928.t1;Parent=MS.gene30928.t1
Gene Sequence

>MS.gene30928

ATGCACTTGTGTTTCCTGATCTGGAAAAAATTTGATCAGAGAAGGGACCAAGAGTGTTACATATTAAACTATCAATGTTACAAGCCAAGTGATGAAAGAAAACTTGGAAGTGAACAATGTGGCAAGATTATAGGAAACAATGAGAATATTGGTCTTGATGAGTGCAAGTTCTTCCTCAAAGCCGTTGTCAACTCAGGCATTGGTGAGGAAACTTATGCTCCAAGAAACTTCATCGAAGGCCGAACTGTGAACCCAACGATGGAAGACGAAGTGGAGGAAATGGAAGAGTTTTGTAATGATAGCATTGCAAAGCTTCTAAACAAATCAGGCATTTCACCTTCGGAGATCGATATTCTTGTGGTAAATGTATCTTTATTCTCTTCTGTGCCTTCTTTAACTTCAAGAATCATTAACCATTACAAAATGAGAGAGGATATAAAAGCTTATAACCTATCTGGTATGGGTTGTAGTGCTAGTCTAATCTCATTAGACGTTATTCAAAACATTTTCAAGTCACAAAAGAACAAGTATGCTCTTTTGTTAACATCAGAGTCTCTTAGTACAAATTGGTATTCAGGAAACAATAGGTCAATGATCCTTGCAAATTGTCTTTTCCGTACCGGCGGTTGTGCAATTCTTCTCACAAATAAAAGATCCTTAAAACACAAAGCTATACTGAAGTTGAAGTGTCTAGTTAGAACTCATCATGGTGCTAGAGATGATGCTCATAACTGTTGTAGTCAAAAAGAAGATGAACAAGGTAAACTTGGTTTTTACCTAGCTAAAGATCTTCCAAAAGCAGCTACAAGAGCTTTTACTGATAATTTAAGGGTGTTATCACCTAAAATTTTACCAGCTAGGGAAATATTTAGGTATATGCTTATGTCACTCATAAAAAAAGTGATAAAATATTTTGCTCCTAAGTCAGCTGCAAGATGGTTTGGAACAAGTAACAAATCACCAGTGAATTTCAAAACAGGTGTTGATCATTTTGTTCTTCACACTGGAGGAAAAGCAGTGATAGATGGAATTGGGATGAGTTTAGATCTGAATGATTATGACCTAGAACCAGCAAGAATGTCATTACATAGATTTGGTAATACATCAGCAAGTAGTTTGTGGTATGTGTTGGGGTATATGGAAGCTAAGAAGAGGTTGAAAAAAGGTGAGAGAGTATTGATGATAAGTTTAGGTGCTGGCTTTAAATGCAACAGTTGTTATTGGGAAGTAATGAGAGATGTTGTTGGTGACGATGGAAATGTGTGGGACGATTGCATTGATTACTATCCACCACACTCTTTGGCCAACCCATTCATGGAGAAGTACGGTTGGATTAACAACATTCAAGATCCAAACACTTTCAAACTCAAACTTGCTGAGTTTCTTCTCAGTCTTAAGTGA
Protein sequence

>MS.gene30928.t1

MHLCFLIWKKFDQRRDQECYILNYQCYKPSDERKLGSEQCGKIIGNNENIGLDECKFFLKAVVNSGIGEETYAPRNFIEGRTVNPTMEDEVEEMEEFCNDSIAKLLNKSGISPSEIDILVVNVSLFSSVPSLTSRIINHYKMREDIKAYNLSGMGCSASLISLDVIQNIFKSQKNKYALLLTSESLSTNWYSGNNRSMILANCLFRTGGCAILLTNKRSLKHKAILKLKCLVRTHHGARDDAHNCCSQKEDEQGKLGFYLAKDLPKAATRAFTDNLRVLSPKILPAREIFRYMLMSLIKKVIKYFAPKSAARWFGTSNKSPVNFKTGVDHFVLHTGGKAVIDGIGMSLDLNDYDLEPARMSLHRFGNTSASSLWYVLGYMEAKKRLKKGERVLMISLGAGFKCNSCYWEVMRDVVGDDGNVWDDCIDYYPPHSLANPFMEKYGWINNIQDPNTFKLKLAEFLLSLK