AlfalfaGEDB | MS.gene049577

M. sativa cultivar XinJiangDaYe / MS.gene049577

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008168 GO:0016740 GO:0016741
PFAM: PF03492.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049577.t1	RHN67909.1	96.2	339	10	1	1	339	7	342	1.40E-183	652.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049577.t1	Q9AVK0	43.1	341	183	4	2	337	31	365	1.3e-73	278.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049577.t1	A0A072V8J9	96.2	339	10	1	1	339	54	389	1.0e-183	652.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049577	MS.gene05137	0.930377	1.79E-93	-1.69E-46
MS.gene049577	MS.gene058142	0.802573	5.26E-49	-1.69E-46
MS.gene049577	MS.gene064919	0.808478	3.04E-50	-1.69E-46
MS.gene049577	MS.gene07055	0.800526	1.38E-48	-1.69E-46
MS.gene049577	MS.gene070829	0.834027	3.80E-56	-1.69E-46
MS.gene049577	MS.gene070831	0.824649	7.20E-54	-1.69E-46
MS.gene049577	MS.gene21907	0.805735	1.16E-49	-1.69E-46
MS.gene049577	MS.gene22505	0.81206	5.13E-51	-1.69E-46
MS.gene049577	MS.gene30928	0.81395	1.97E-51	-1.69E-46
MS.gene049577	MS.gene31182	0.804147	2.48E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene000556	MS.gene049577	PPI
MS.gene008450	MS.gene049577	PPI
MS.gene010553	MS.gene049577	PPI
MS.gene012028	MS.gene049577	PPI
MS.gene014262	MS.gene049577	PPI
MS.gene02421	MS.gene049577	PPI
MS.gene024299	MS.gene049577	PPI
MS.gene024301	MS.gene049577	PPI
MS.gene024314	MS.gene049577	PPI
MS.gene024315	MS.gene049577	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049577.t1	MTR_3g467560	96.165	339	10	1	1	339	54	389	0.0	667
MS.gene049577.t1	MTR_7g084620	77.876	339	68	4	1	337	30	363	0.0	539
MS.gene049577.t1	MTR_7g084350	70.326	337	99	1	1	337	28	363	9.06e-179	499
MS.gene049577.t1	MTR_7g084330	70.206	339	79	5	1	337	13	331	4.72e-168	471
MS.gene049577.t1	MTR_3g117060	54.464	336	146	4	6	337	35	367	4.14e-127	369
MS.gene049577.t1	MTR_8g076900	44.512	328	167	5	15	337	43	360	2.72e-90	275
MS.gene049577.t1	MTR_8g077040	40.988	344	183	6	5	337	34	368	1.84e-89	273
MS.gene049577.t1	MTR_5g020940	43.939	330	167	5	19	337	57	379	1.46e-88	271
MS.gene049577.t1	MTR_6g056070	42.138	318	165	4	24	337	53	355	4.80e-87	266
MS.gene049577.t1	MTR_4g018820	40.356	337	184	6	5	337	34	357	3.30e-85	261

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049577.t1	AT1G19640	40.678	354	189	5	6	338	35	388	2.58e-77	242
MS.gene049577.t1	AT5G66430	41.231	325	172	5	2	319	23	335	5.99e-77	240
MS.gene049577.t1	AT5G38020	41.768	328	166	7	6	319	32	348	1.02e-76	240
MS.gene049577.t1	AT3G11480	41.840	337	176	5	6	337	57	378	6.25e-76	238
MS.gene049577.t1	AT5G38020	42.188	320	160	7	14	319	1	309	1.33e-75	236
MS.gene049577.t1	AT5G04370	40.988	344	178	7	2	337	63	389	2.63e-71	227
MS.gene049577.t1	AT5G04370	41.056	341	182	7	2	337	68	394	2.98e-71	227
MS.gene049577.t1	AT5G04370	41.056	341	182	7	2	337	82	408	3.70e-71	227
MS.gene049577.t1	AT3G21950	40.968	310	154	7	20	313	46	342	3.69e-69	221
MS.gene049577.t1	AT4G36470	39.329	328	172	8	26	339	57	371	4.46e-68	218

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Find 54 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAGTTGATATAATTAG+TGG	0.455446	3.2:-58371410	None:intergenic
AAAGTTGTGTTGGACATCAA+CGG	0.558540	3.2:-58371106	None:intergenic
AAATATGCTTTATACACTGA+TGG	0.545387	3.2:-58372662	None:intergenic
AACATACCATCCTACTGTAG+AGG	0.616142	3.2:+58373484	MS.gene049577:CDS
AAGAAGTTGATATAATTAGT+GGG	0.594084	3.2:-58371409	None:intergenic
AAGAGTAGACAACAAGAGAA+AGG	0.535243	3.2:+58371325	MS.gene049577:CDS
AATAGTATCAGATGGTATGA+TGG	0.539204	3.2:+58372736	MS.gene049577:CDS
AATGAAAGTGGCAGCTATAA+TGG	0.330658	3.2:+58371069	None:intergenic
AATGCAAAACCACTCAACAA+GGG	0.683152	3.2:+58372611	MS.gene049577:CDS
ACAACCATTAAAAGTTGTGT+TGG	0.517417	3.2:-58371116	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATAAATAAATATGGGGGG+GGG	-	chr3.2:58371988-58372007	None:intergenic	30.0%
AACAAAGATAAAGAGAGTAG+AGG	-	chr3.2:58372354-58372373	None:intergenic	30.0%
AATAGTATCAGATGGTATGA+TGG	+	chr3.2:58372736-58372755	MS.gene049577:CDS	30.0%
AATGATAGACATGTGATATG+AGG	-	chr3.2:58372931-58372950	None:intergenic	30.0%
ACAACCATTAAAAGTTGTGT+TGG	-	chr3.2:58371119-58371138	None:intergenic	30.0%
ACTAAACCCTGCAAATTATA+AGG	-	chr3.2:58373431-58373450	None:intergenic	30.0%
AGAAAACATCTATTGTGAGA+CGG	-	chr3.2:58373091-58373110	None:intergenic	30.0%
AGAACAATCATTAACCAAGA+AGG	+	chr3.2:58371574-58371593	MS.gene049577:intron	30.0%
AGGCTCATATCATATTATCA+AGG	+	chr3.2:58371594-58371613	MS.gene049577:intron	30.0%
ATCGTTCATTTGAGAACAAA+CGG	+	chr3.2:58371691-58371710	MS.gene049577:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	58371070	58373808	58371070	ID=MS.gene049577
chr3.2	mRNA	58371070	58373808	58371070	ID=MS.gene049577.t1;Parent=MS.gene049577
chr3.2	exon	58371070	58371465	58371070	ID=MS.gene049577.t1.exon1;Parent=MS.gene049577.t1
chr3.2	CDS	58371070	58371465	58371070	ID=cds.MS.gene049577.t1;Parent=MS.gene049577.t1
chr3.2	exon	58372590	58372847	58372590	ID=MS.gene049577.t1.exon2;Parent=MS.gene049577.t1
chr3.2	CDS	58372590	58372847	58372590	ID=cds.MS.gene049577.t1;Parent=MS.gene049577.t1
chr3.2	exon	58373443	58373808	58373443	ID=MS.gene049577.t1.exon3;Parent=MS.gene049577.t1
chr3.2	CDS	58373443	58373808	58373443	ID=cds.MS.gene049577.t1;Parent=MS.gene049577.t1

Gene Sequence

>MS.gene049577

ATGAAAGTGGCAGCTATAATGGAAGAGAATTTAAAACCGTTGATGTCCAACACAACTTTTAATGGTTGTTTTAAAGTAGCTGATTTAGGTTGCTCTTCGGGGCCAAACACACTTTTGGTCACCTCTAATATCTTGAACATCATACGTGAAATTAGCTTGAAGTTAAACCATGAGACACCTGTATTTCAAATTTTTCTCAATGATTTATTTGAAAATGATTTTAGTACAATCTTCAAGTTACTTCCTGATTTCTACAAGAGTAGACAACAAGAGAAAGGAGAAAATGTTGGAGCATGCTTCATAAATGCAACACCCGGAAATTTTTATGGACGACTCTTTCCCACTAATTATATCAACTTCTTTCATTCCTCCTATTGTCTACATTGGCTATCACAAGTAAGTACTACAAAACCAAGATTTAAATTTAAGCATGGATTTTGTTATTCAACAATAGGGTACCCTTAGGATGGTCAAGTAATGTTTTAGACCAAGTTGGTCCAACTAAGAACAATCATTAACCAAGAAGGCTCATATCATATTATCAAGGTCTAGCCACACTAGATCCACACCTTCTCTATTCTGATTTGGTGTTGTAAAAAATGAACGTAGTTTTCGATCTCGATCGTTCATTTGAGAACAAACGGATAAAATTGAAAACTGTGTCTTTTTAATAAAATGATCTTAGTCGTACAATTTTGATTGGACGACTCACATCCTGTTCAGGAAATCTGCTTTCTCCTTCTATCACCATCGATCAAGTAGGATCATAACAACAAAGCATAACTAACCCTTTTTATTAATTTTTTTTTTAAAGAACTAACTCATATACAAGATGAGATCAACACTCAACACCATGGATGGGTTACCCCGTTGAAGTCAACTCTCAACACCATGGATGGGTCCCCCCCCCCCCCCCCCCCCCCCATATTTATTTATTTTAACTTTATATGATGTTGTATAATAGATTACCTTATAGTTTTTAGGCGTAAATGCATTAATCTTTTACTAAAATCCTTAAAAGACAAAAAGAAAGTGAAGTTTTAATTTGTGTGCAACTTACAAAAAAAAAAAAAAATCATAATCCTTAACTAGAATCACAAACAAATTTCTAGGTGAAATTGCAATGAGGAATCTTCAATTGAGATTCTAGTTAAGGATTATGATTTTTTTTGTGCGTTGCATACAAATTAAAACTTCACTTTCTTTTTGTCTTTTAAGGATTTTAGAAAAAGATTAATGCATTTAAACCTAAAAACTAGTTAATCAATCATGGTGTTTGTTCCTCTACTCTCTTTATCTTTGTTCTTTAATTTGATTGGATTTGTTATACACTTGCTTGCTAGATTTGGTCTTGGTTGAATATTGTTGCTTTGACTGTTTTGTTGTTTATTGCAATCTTTATTTGTTCCACATGTTGTCTTGTCGATTTTGCACAAGAGTTCGAATTCACACCAGTTTTTAAGTAATATTCCGTATAATATAAATTATTTATATTTGAAGTTCATCTTTTATGGCATTAGGCACCCAAAGATTTGGCCAATAATGCAAAACCACTCAACAAGGGAAATATTTACATATCAAGAACAAGTCCTCCATCAGTGTATAAAGCATATTTTGTGCAATTTGCAAGAGATTTCAAGTGTTTTCTAAAGTCACGCTTCGAAGAAATAGTATCAGATGGTATGATGGTACTAACATTCATTGGCAGAGAAACATCAAATGAAATTACTTCTATTCAAGAGGTACTTGGCATGGTACTCAACGAGATGGTCCAAGAGGTAAATATAAGCTTTGATAGCAAGTAAGAAAGAGTGCCTAAAAATAAAAATTAAACATTTCAAAACAAAAATCTTATCGGCCTCATATCACATGTCTATCATTTCACACACACACTTTAAATACCCTAGTTTCCTTTTACAATAGTTTAAACCTTTTATAAATTTATATTGATATGATAAAATACCATATAACTAGTTATTCACGTTTAAAAATATGGTTGATTCAGATGAGTACTTCCTCCGTCTCACAATAGATGTTTTCTTTCCTGATTTTTTTTTTGTCTCTTTTATAATATAGATACAACATTTAATACTTTTCTCCACTTACAAACCCTTATTTATTGCATTTTAATCATCTAAAACTATTATTCCACTAACCATAATTAATAAGAATGTTTTAGAAAATGATATTGTACATTTTGCCATTAAAATCAACACTATTAATCATTTCTTTAATTACCGTGTAAAACTCATAGTTTGTTATTATTGAACGACATTATATTATAGGAGACTTAATTTACACAACAAAATAAATATTTAGTTTTTCTTTAACTTTTGATTTTCCTTTTTTATAAAGATTCCTTATAATTTGCAGGGTTTAGTTGAAGAGGAGAAATTAGACTTGTTTAATTTTCCAACATACCATCCTACTGTAGAGGAAGTAAGACAAGTGATTGAGGCAGAAGGATCTTTTACACTTCAAGCAATGAAAACTTTTAAAATGGGTTGGGATGCAAACCTCCATAATGATATTGTTGATGGTATTGTTGATAGCAAAATGAGAGGGGAGCTTATAGCCAAGTACCACAGAGCTGTTTTTGAACCTCTTTTAATAGACGGGTTTGGTGAAAATATCATGGATGAATTGTTCTCAAGGTTTGCTAAGCTGCTTGCACAACTCATTGAGTTAGAGACATTAGAGTTTACTAATATTGTATTGCTCTTGTCAAAGGGTCTTTGA

Protein sequence

>MS.gene049577.t1

MKVAAIMEENLKPLMSNTTFNGCFKVADLGCSSGPNTLLVTSNILNIIREISLKLNHETPVFQIFLNDLFENDFSTIFKLLPDFYKSRQQEKGENVGACFINATPGNFYGRLFPTNYINFFHSSYCLHWLSQAPKDLANNAKPLNKGNIYISRTSPPSVYKAYFVQFARDFKCFLKSRFEEIVSDGMMVLTFIGRETSNEITSIQEVLGMVLNEMVQEGLVEEEKLDLFNFPTYHPTVEEVRQVIEAEGSFTLQAMKTFKMGWDANLHNDIVDGIVDSKMRGELIAKYHRAVFEPLLIDGFGENIMDELFSRFAKLLAQLIELETLEFTNIVLLLSKGL