Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32000.t1 | XP_024638882.1 | 97.5 | 279 | 7 | 0 | 1 | 279 | 1 | 279 | 2.20E-150 | 541.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32000.t1 | A0A396I9U6 | 97.5 | 279 | 7 | 0 | 1 | 279 | 1 | 279 | 1.6e-150 | 541.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene32000 | 0.829643 | 4.59E-55 | -1.69E-46 |
| MS.gene049151 | MS.gene32000 | 0.810186 | 1.31E-50 | -1.69E-46 |
| MS.gene049153 | MS.gene32000 | 0.840818 | 6.92E-58 | -1.69E-46 |
| MS.gene050398 | MS.gene32000 | 0.801743 | 7.80E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene32000 | 0.825964 | 3.52E-54 | -1.69E-46 |
| MS.gene052255 | MS.gene32000 | 0.812624 | 3.86E-51 | -1.69E-46 |
| MS.gene053074 | MS.gene32000 | 0.844002 | 9.90E-59 | -1.69E-46 |
| MS.gene053204 | MS.gene32000 | 0.804617 | 1.98E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene32000 | 0.814142 | 1.79E-51 | -1.69E-46 |
| MS.gene053928 | MS.gene32000 | 0.809062 | 2.28E-50 | -1.69E-46 |
| MS.gene054184 | MS.gene32000 | 0.821857 | 3.23E-53 | -1.69E-46 |
| MS.gene054185 | MS.gene32000 | 0.823623 | 1.25E-53 | -1.69E-46 |
| MS.gene054596 | MS.gene32000 | 0.824227 | 9.05E-54 | -1.69E-46 |
| MS.gene055604 | MS.gene32000 | 0.800299 | 1.54E-48 | -1.69E-46 |
| MS.gene057121 | MS.gene32000 | 0.801513 | 8.69E-49 | -1.69E-46 |
| MS.gene057750 | MS.gene32000 | 0.810083 | 1.38E-50 | -1.69E-46 |
| MS.gene057754 | MS.gene32000 | 0.847964 | 8.29E-60 | -1.69E-46 |
| MS.gene05811 | MS.gene32000 | 0.80769 | 4.47E-50 | -1.69E-46 |
| MS.gene059124 | MS.gene32000 | 0.823013 | 1.74E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32000.t1 | MTR_4g088515 | 97.388 | 268 | 7 | 0 | 1 | 268 | 1 | 268 | 0.0 | 543 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32000.t1 | AT4G26415 | 45.299 | 234 | 61 | 10 | 3 | 192 | 7 | 217 | 3.30e-44 | 152 |
Find 58 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCTCCTCAAGAACTCTT+TGG | 0.228130 | 4.3:+24755051 | MS.gene32000:CDS |
| TCAATGAATATAGTGGAATA+AGG | 0.272127 | 4.3:+24755320 | MS.gene32000:CDS |
| GTCATTTGAGAGGCATGATT+TGG | 0.298535 | 4.3:+24755696 | MS.gene32000:CDS |
| TCCATGTATAGGTCATCTTT+TGG | 0.313265 | 4.3:+24755436 | MS.gene32000:CDS |
| TCTTATGCCTTTCTTCATCT+TGG | 0.319608 | 4.3:-24755077 | None:intergenic |
| TTTCTCCTCAAGAACTCTTT+GGG | 0.349291 | 4.3:+24755052 | MS.gene32000:CDS |
| AGGTGTTGTGGCAAAGCTAA+TGG | 0.356397 | 4.3:+24755576 | MS.gene32000:CDS |
| AACCAACAAAATAGTACTAT+TGG | 0.366556 | 4.3:+24755136 | MS.gene32000:CDS |
| TGGATATTGTATTATGAAAC+CGG | 0.367697 | 4.3:+24755801 | MS.gene32000:CDS |
| GGATGAGTAGTGTTGATTGT+AGG | 0.385703 | 4.3:+24755653 | MS.gene32000:CDS |
| CAATGAATATAGTGGAATAA+GGG | 0.392950 | 4.3:+24755321 | MS.gene32000:CDS |
| AGAACCAAAGCTTGATGAAA+AGG | 0.407802 | 4.3:+24755501 | MS.gene32000:CDS |
| GCCATAGCTTCTAGAGAAAC+CGG | 0.424661 | 4.3:-24755820 | None:intergenic |
| GGTGTTGTGGCAAAGCTAAT+GGG | 0.440558 | 4.3:+24755577 | MS.gene32000:CDS |
| TCTTATACTACAATTAGAAA+TGG | 0.443850 | 4.3:+24755270 | MS.gene32000:CDS |
| CTCCTTGGTTGCCAACTACT+AGG | 0.453422 | 4.3:-24755850 | None:intergenic |
| TGTGGCAAAGCTAATGGGTT+TGG | 0.466899 | 4.3:+24755582 | MS.gene32000:CDS |
| GTTAGAAGAAGAAATAGGTC+TGG | 0.472977 | 4.3:+24755763 | MS.gene32000:CDS |
| AGTATAAGATCTTCTAATGT+TGG | 0.477524 | 4.3:-24755256 | None:intergenic |
| TGCTGCTTGTGATGTCAACT+TGG | 0.485632 | 4.3:-24755180 | None:intergenic |
| AGTACTATTGGCAATGGCAT+TGG | 0.489373 | 4.3:+24755148 | MS.gene32000:CDS |
| CTAATCTAAACATATCTCCT+TGG | 0.491234 | 4.3:-24755865 | None:intergenic |
| AAAACTAGCAATGGACATGC+AGG | 0.491726 | 4.3:+24755723 | MS.gene32000:CDS |
| CCCAAAAGATGACCTATACA+TGG | 0.498041 | 4.3:-24755437 | None:intergenic |
| TTGTGATGTCAACTTGGCTA+TGG | 0.503039 | 4.3:-24755174 | None:intergenic |
| ATTAGCTTTGCCACAACACC+TGG | 0.503693 | 4.3:-24755574 | None:intergenic |
| TTCTCCCCAGCAACTGTTGG+TGG | 0.503702 | 4.3:-24755535 | None:intergenic |
| GTAGGAGACAAAATATAAGA+AGG | 0.511223 | 4.3:+24755671 | MS.gene32000:CDS |
| ATGGAAGAGATTCCATGTAT+AGG | 0.512387 | 4.3:+24755425 | MS.gene32000:CDS |
| TGGCAGGAGGTCCTAGTAGT+TGG | 0.521625 | 4.3:+24755839 | MS.gene32000:CDS |
| ACCGGTTTCTCTAGAAGCTA+TGG | 0.522607 | 4.3:+24755819 | MS.gene32000:CDS |
| TTGCTGGGGAGAACGTGGTT+TGG | 0.527912 | 4.3:+24755545 | MS.gene32000:CDS |
| ACATTGTTAGAAGAAGAAAT+AGG | 0.529346 | 4.3:+24755758 | MS.gene32000:CDS |
| ATGCTTTGAATCAATATCCA+AGG | 0.531501 | 4.3:+24755392 | MS.gene32000:CDS |
| AAATGGAGGAAGAGATGGCA+AGG | 0.533678 | 4.3:+24755287 | MS.gene32000:CDS |
| ATTAGAAATGGAGGAAGAGA+TGG | 0.541356 | 4.3:+24755282 | MS.gene32000:CDS |
| GAGTTCTTGAGGAGAAAGAA+TGG | 0.549925 | 4.3:-24755046 | None:intergenic |
| TTGGGAGCCAAGATGAAGAA+AGG | 0.561593 | 4.3:+24755070 | MS.gene32000:CDS |
| CAAAATAGTACTATTGGCAA+TGG | 0.563916 | 4.3:+24755142 | MS.gene32000:CDS |
| CTTCCATCAAGTGAAAACCT+TGG | 0.575355 | 4.3:-24755409 | None:intergenic |
| GTCCTAGTAGTTGGCAACCA+AGG | 0.577078 | 4.3:+24755848 | MS.gene32000:CDS |
| AATGAATATAGTGGAATAAG+GGG | 0.579962 | 4.3:+24755322 | MS.gene32000:CDS |
| TATACTACAATTAGAAATGG+AGG | 0.596710 | 4.3:+24755273 | MS.gene32000:CDS |
| TGGCTCCCAAAGAGTTCTTG+AGG | 0.597986 | 4.3:-24755057 | None:intergenic |
| GAAGCAATTCCAGTGTCAGT+AGG | 0.604697 | 4.3:+24755604 | MS.gene32000:CDS |
| TTTGGAGAGAAAACTAGCAA+TGG | 0.605568 | 4.3:+24755714 | MS.gene32000:CDS |
| GTTTCTCTAGAAGCTATGGC+AGG | 0.610709 | 4.3:+24755823 | MS.gene32000:CDS |
| AACAGTTGCTGGGGAGAACG+TGG | 0.612915 | 4.3:+24755540 | MS.gene32000:CDS |
| AACGTGGTTTGGTGTAAACC+AGG | 0.613124 | 4.3:+24755556 | MS.gene32000:CDS |
| AATATAGTGGAATAAGGGGA+AGG | 0.620558 | 4.3:+24755326 | MS.gene32000:CDS |
| TAAGAAGGAGGTCATTTGAG+AGG | 0.634095 | 4.3:+24755686 | MS.gene32000:CDS |
| GGAGACAAAATATAAGAAGG+AGG | 0.634894 | 4.3:+24755674 | MS.gene32000:CDS |
| CTGACATATTAAGATCTGCA+AGG | 0.639107 | 4.3:+24755368 | MS.gene32000:CDS |
| AGTTGACATCACAAGCAGCA+AGG | 0.656859 | 4.3:+24755183 | MS.gene32000:CDS |
| ACGTTCTCCCCAGCAACTGT+TGG | 0.657823 | 4.3:-24755538 | None:intergenic |
| TCTCTAGAAGCTATGGCAGG+AGG | 0.665560 | 4.3:+24755826 | MS.gene32000:CDS |
| AAACTAGCAATGGACATGCA+GGG | 0.676573 | 4.3:+24755724 | MS.gene32000:CDS |
| TTGGTGTAAACCAGGTGTTG+TGG | 0.704435 | 4.3:+24755564 | MS.gene32000:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTATACTACAATTAGAAA+TGG | + | chr4.3:24755270-24755289 | MS.gene32000:CDS | 20.0% |
| ! | AACCAACAAAATAGTACTAT+TGG | + | chr4.3:24755136-24755155 | MS.gene32000:CDS | 25.0% |
| ! | ACATTGTTAGAAGAAGAAAT+AGG | + | chr4.3:24755758-24755777 | MS.gene32000:CDS | 25.0% |
| ! | AGTATAAGATCTTCTAATGT+TGG | - | chr4.3:24755259-24755278 | None:intergenic | 25.0% |
| ! | GAAAAACTCAATGAATATAG+TGG | + | chr4.3:24755313-24755332 | MS.gene32000:CDS | 25.0% |
| ! | TATACTACAATTAGAAATGG+AGG | + | chr4.3:24755273-24755292 | MS.gene32000:CDS | 25.0% |
| ! | TGGATATTGTATTATGAAAC+CGG | + | chr4.3:24755801-24755820 | MS.gene32000:CDS | 25.0% |
| !! | AATGAATATAGTGGAATAAG+GGG | + | chr4.3:24755322-24755341 | MS.gene32000:CDS | 25.0% |
| !! | CAATGAATATAGTGGAATAA+GGG | + | chr4.3:24755321-24755340 | MS.gene32000:CDS | 25.0% |
| !! | TCAATGAATATAGTGGAATA+AGG | + | chr4.3:24755320-24755339 | MS.gene32000:CDS | 25.0% |
| AAAGATCTGACAAAAAAGAG+AGG | + | chr4.3:24755632-24755651 | MS.gene32000:CDS | 30.0% | |
| AATTGTCACATTGCAAAAAC+GGG | - | chr4.3:24755216-24755235 | None:intergenic | 30.0% | |
| CAAAATAGTACTATTGGCAA+TGG | + | chr4.3:24755142-24755161 | MS.gene32000:CDS | 30.0% | |
| GAATTGTCACATTGCAAAAA+CGG | - | chr4.3:24755217-24755236 | None:intergenic | 30.0% | |
| GTAGGAGACAAAATATAAGA+AGG | + | chr4.3:24755671-24755690 | MS.gene32000:CDS | 30.0% | |
| ! | ATAACCTTTTCATCAAGCTT+TGG | - | chr4.3:24755508-24755527 | None:intergenic | 30.0% |
| ! | ATGCTTTGAATCAATATCCA+AGG | + | chr4.3:24755392-24755411 | MS.gene32000:CDS | 30.0% |
| ! | TGCCAATAGTACTATTTTGT+TGG | - | chr4.3:24755141-24755160 | None:intergenic | 30.0% |
| !!! | ATTTTGTTGGTTTAGAGTTG+AGG | - | chr4.3:24755128-24755147 | None:intergenic | 30.0% |
| !!! | TCTGGTTTTTGCTCTAAAAA+TGG | + | chr4.3:24755781-24755800 | MS.gene32000:CDS | 30.0% |
| AGAACCAAAGCTTGATGAAA+AGG | + | chr4.3:24755501-24755520 | MS.gene32000:CDS | 35.0% | |
| ATGGAAGAGATTCCATGTAT+AGG | + | chr4.3:24755425-24755444 | MS.gene32000:CDS | 35.0% | |
| ATTAGAAATGGAGGAAGAGA+TGG | + | chr4.3:24755282-24755301 | MS.gene32000:CDS | 35.0% | |
| ATTGTCACATTGCAAAAACG+GGG | - | chr4.3:24755215-24755234 | None:intergenic | 35.0% | |
| CTGACATATTAAGATCTGCA+AGG | + | chr4.3:24755368-24755387 | MS.gene32000:CDS | 35.0% | |
| GGAGACAAAATATAAGAAGG+AGG | + | chr4.3:24755674-24755693 | MS.gene32000:CDS | 35.0% | |
| GTTAGAAGAAGAAATAGGTC+TGG | + | chr4.3:24755763-24755782 | MS.gene32000:CDS | 35.0% | |
| TCTTATGCCTTTCTTCATCT+TGG | - | chr4.3:24755080-24755099 | None:intergenic | 35.0% | |
| TTTCTCCTCAAGAACTCTTT+GGG | + | chr4.3:24755052-24755071 | MS.gene32000:CDS | 35.0% | |
| TTTGGAGAGAAAACTAGCAA+TGG | + | chr4.3:24755714-24755733 | MS.gene32000:CDS | 35.0% | |
| ! | AATATAGTGGAATAAGGGGA+AGG | + | chr4.3:24755326-24755345 | MS.gene32000:CDS | 35.0% |
| ! | CCATGTATAGGTCATCTTTT+GGG | + | chr4.3:24755437-24755456 | MS.gene32000:CDS | 35.0% |
| ! | TATCCAAGGTTTTCACTTGA+TGG | + | chr4.3:24755406-24755425 | MS.gene32000:CDS | 35.0% |
| ! | TCCATGTATAGGTCATCTTT+TGG | + | chr4.3:24755436-24755455 | MS.gene32000:CDS | 35.0% |
| !!! | TCATCTTTTGGGACTATAGA+AGG | + | chr4.3:24755448-24755467 | MS.gene32000:CDS | 35.0% |
| AAAACTAGCAATGGACATGC+AGG | + | chr4.3:24755723-24755742 | MS.gene32000:CDS | 40.0% | |
| AAACTAGCAATGGACATGCA+GGG | + | chr4.3:24755724-24755743 | MS.gene32000:CDS | 40.0% | |
| AGTACTATTGGCAATGGCAT+TGG | + | chr4.3:24755148-24755167 | MS.gene32000:CDS | 40.0% | |
| CCCAAAAGATGACCTATACA+TGG | - | chr4.3:24755440-24755459 | None:intergenic | 40.0% | |
| CTTCCATCAAGTGAAAACCT+TGG | - | chr4.3:24755412-24755431 | None:intergenic | 40.0% | |
| CTTTCTCCTCAAGAACTCTT+TGG | + | chr4.3:24755051-24755070 | MS.gene32000:CDS | 40.0% | |
| GAGTTCTTGAGGAGAAAGAA+TGG | - | chr4.3:24755049-24755068 | None:intergenic | 40.0% | |
| TAAGAAGGAGGTCATTTGAG+AGG | + | chr4.3:24755686-24755705 | MS.gene32000:CDS | 40.0% | |
| TTGTGATGTCAACTTGGCTA+TGG | - | chr4.3:24755177-24755196 | None:intergenic | 40.0% | |
| ! | CTTTTTCTACCTACTGACAC+TGG | - | chr4.3:24755616-24755635 | None:intergenic | 40.0% |
| ! | GTCATTTGAGAGGCATGATT+TGG | + | chr4.3:24755696-24755715 | MS.gene32000:CDS | 40.0% |
| !! | GGATGAGTAGTGTTGATTGT+AGG | + | chr4.3:24755653-24755672 | MS.gene32000:CDS | 40.0% |
| AAATGGAGGAAGAGATGGCA+AGG | + | chr4.3:24755287-24755306 | MS.gene32000:CDS | 45.0% | |
| AGTTGACATCACAAGCAGCA+AGG | + | chr4.3:24755183-24755202 | MS.gene32000:CDS | 45.0% | |
| GAAGCAATTCCAGTGTCAGT+AGG | + | chr4.3:24755604-24755623 | MS.gene32000:CDS | 45.0% | |
| TTGGGAGCCAAGATGAAGAA+AGG | + | chr4.3:24755070-24755089 | MS.gene32000:CDS | 45.0% | |
| ! | ACCGGTTTCTCTAGAAGCTA+TGG | + | chr4.3:24755819-24755838 | MS.gene32000:CDS | 45.0% |
| ! | AGGTGTTGTGGCAAAGCTAA+TGG | + | chr4.3:24755576-24755595 | MS.gene32000:CDS | 45.0% |
| ! | GCCATAGCTTCTAGAGAAAC+CGG | - | chr4.3:24755823-24755842 | None:intergenic | 45.0% |
| ! | GGTGTTGTGGCAAAGCTAAT+GGG | + | chr4.3:24755577-24755596 | MS.gene32000:CDS | 45.0% |
| ! | GTTTCTCTAGAAGCTATGGC+AGG | + | chr4.3:24755823-24755842 | MS.gene32000:CDS | 45.0% |
| ! | GTTTTCCACCAACAGTTGCT+GGG | + | chr4.3:24755530-24755549 | MS.gene32000:CDS | 45.0% |
| ! | TGCTGCTTGTGATGTCAACT+TGG | - | chr4.3:24755183-24755202 | None:intergenic | 45.0% |
| ! | TGTTTTCCACCAACAGTTGC+TGG | + | chr4.3:24755529-24755548 | MS.gene32000:CDS | 45.0% |
| ! | TTGGTGTAAACCAGGTGTTG+TGG | + | chr4.3:24755564-24755583 | MS.gene32000:CDS | 45.0% |
| ! | TTTTCCACCAACAGTTGCTG+GGG | + | chr4.3:24755531-24755550 | MS.gene32000:CDS | 45.0% |
| !! | AACGTGGTTTGGTGTAAACC+AGG | + | chr4.3:24755556-24755575 | MS.gene32000:CDS | 45.0% |
| !! | ATTAGCTTTGCCACAACACC+TGG | - | chr4.3:24755577-24755596 | None:intergenic | 45.0% |
| !! | TGTGGCAAAGCTAATGGGTT+TGG | + | chr4.3:24755582-24755601 | MS.gene32000:CDS | 45.0% |
| CTCCTTGGTTGCCAACTACT+AGG | - | chr4.3:24755853-24755872 | None:intergenic | 50.0% | |
| GTCCTAGTAGTTGGCAACCA+AGG | + | chr4.3:24755848-24755867 | MS.gene32000:CDS | 50.0% | |
| TGGCTCCCAAAGAGTTCTTG+AGG | - | chr4.3:24755060-24755079 | None:intergenic | 50.0% | |
| ! | TCTCTAGAAGCTATGGCAGG+AGG | + | chr4.3:24755826-24755845 | MS.gene32000:CDS | 50.0% |
| AACAGTTGCTGGGGAGAACG+TGG | + | chr4.3:24755540-24755559 | MS.gene32000:CDS | 55.0% | |
| ACGTTCTCCCCAGCAACTGT+TGG | - | chr4.3:24755541-24755560 | None:intergenic | 55.0% | |
| TGGCAGGAGGTCCTAGTAGT+TGG | + | chr4.3:24755839-24755858 | MS.gene32000:CDS | 55.0% | |
| TTCTCCCCAGCAACTGTTGG+TGG | - | chr4.3:24755538-24755557 | None:intergenic | 55.0% | |
| TTGCTGGGGAGAACGTGGTT+TGG | + | chr4.3:24755545-24755564 | MS.gene32000:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 24755043 | 24755882 | 24755043 | ID=MS.gene32000 |
| chr4.3 | mRNA | 24755043 | 24755882 | 24755043 | ID=MS.gene32000.t1;Parent=MS.gene32000 |
| chr4.3 | exon | 24755043 | 24755882 | 24755043 | ID=MS.gene32000.t1.exon1;Parent=MS.gene32000.t1 |
| chr4.3 | CDS | 24755043 | 24755882 | 24755043 | ID=cds.MS.gene32000.t1;Parent=MS.gene32000.t1 |
| Gene Sequence |
| Protein sequence |
