AlfalfaGEDB | MS.gene050398

M. sativa cultivar XinJiangDaYe / MS.gene050398

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0008270 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043167 GO:0043169 GO:0043565 GO:0046872 GO:0046914 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00320.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050398.t1	XP_003595135.2	92.1	114	9	0	1	114	77	190	3.00E-50	207.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050398.t1	G7IIE3	92.1	114	9	0	1	114	72	185	2.2e-50	207.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene050398.t1	TF	C2C2-GATA

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049153	MS.gene050398	0.812496	4.12E-51	-1.69E-46
MS.gene050226	MS.gene050398	0.810694	1.01E-50	-1.69E-46
MS.gene050227	MS.gene050398	0.803346	3.64E-49	-1.69E-46
MS.gene050398	MS.gene052077	0.820566	6.41E-53	-1.69E-46
MS.gene050398	MS.gene052255	0.805387	1.37E-49	-1.69E-46
MS.gene050398	MS.gene053928	0.804207	2.41E-49	-1.69E-46
MS.gene050398	MS.gene054349	0.808427	3.11E-50	-1.69E-46
MS.gene050398	MS.gene057750	0.817305	3.53E-52	-1.69E-46
MS.gene050398	MS.gene057754	0.821168	4.66E-53	-1.69E-46
MS.gene050398	MS.gene05907	0.807079	6.02E-50	-1.69E-46
MS.gene050398	MS.gene059421	0.806744	7.09E-50	-1.69E-46
MS.gene050398	MS.gene062226	0.803163	3.97E-49	-1.69E-46
MS.gene050398	MS.gene067385	0.827134	1.85E-54	-1.69E-46
MS.gene050398	MS.gene067552	0.800918	1.15E-48	-1.69E-46
MS.gene050398	MS.gene067553	0.808374	3.20E-50	-1.69E-46
MS.gene050398	MS.gene069870	0.800384	1.48E-48	-1.69E-46
MS.gene050398	MS.gene06990	0.811612	6.42E-51	-1.69E-46
MS.gene050398	MS.gene074393	-0.801294	9.64E-49	-1.69E-46
MS.gene050398	MS.gene08779	0.810587	1.07E-50	-1.69E-46
MS.gene050398	MS.gene09310	0.814324	1.63E-51	-1.69E-46
MS.gene050398	MS.gene20340	0.825352	4.91E-54	-1.69E-46
MS.gene050398	MS.gene20556	0.830757	2.45E-55	-1.69E-46
MS.gene050398	MS.gene22689	0.802167	6.38E-49	-1.69E-46
MS.gene050398	MS.gene22967	0.815946	7.12E-52	-1.69E-46
MS.gene050398	MS.gene23318	0.803175	3.95E-49	-1.69E-46
MS.gene050398	MS.gene25913	-0.800001	1.77E-48	-1.69E-46
MS.gene050398	MS.gene26351	0.822012	2.97E-53	-1.69E-46
MS.gene050398	MS.gene26514	0.800628	1.32E-48	-1.69E-46
MS.gene050398	MS.gene26649	0.805935	1.05E-49	-1.69E-46
MS.gene050398	MS.gene27094	0.806585	7.66E-50	-1.69E-46
MS.gene050398	MS.gene28157	0.81225	4.66E-51	-1.69E-46
MS.gene050398	MS.gene28693	0.818754	1.66E-52	-1.69E-46
MS.gene050398	MS.gene28694	0.824563	7.54E-54	-1.69E-46
MS.gene050398	MS.gene31116	0.807768	4.30E-50	-1.69E-46
MS.gene050398	MS.gene31604	0.818961	1.49E-52	-1.69E-46
MS.gene050398	MS.gene31671	0.818452	1.95E-52	-1.69E-46
MS.gene050398	MS.gene32000	0.801743	7.80E-49	-1.69E-46
MS.gene050398	MS.gene32732	0.804298	2.31E-49	-1.69E-46
MS.gene050398	MS.gene33198	0.821543	3.82E-53	-1.69E-46
MS.gene050398	MS.gene37952	0.80448	2.12E-49	-1.69E-46
MS.gene050398	MS.gene38192	0.800695	1.28E-48	-1.69E-46
MS.gene050398	MS.gene38528	0.800091	1.70E-48	-1.69E-46
MS.gene050398	MS.gene39496	0.849599	2.92E-60	-1.69E-46
MS.gene050398	MS.gene39638	0.800878	1.17E-48	-1.69E-46
MS.gene050398	MS.gene40169	0.833945	3.98E-56	-1.69E-46
MS.gene050398	MS.gene42668	0.820305	7.36E-53	-1.69E-46
MS.gene050398	MS.gene42831	0.803475	3.42E-49	-1.69E-46
MS.gene050398	MS.gene43782	0.802295	6.00E-49	-1.69E-46
MS.gene050398	MS.gene43822	0.804773	1.84E-49	-1.69E-46
MS.gene050398	MS.gene45108	0.808172	3.53E-50	-1.69E-46
MS.gene050398	MS.gene45403	0.823278	1.51E-53	-1.69E-46
MS.gene050398	MS.gene45533	0.816202	6.25E-52	-1.69E-46
MS.gene050398	MS.gene48030	0.814219	1.72E-51	-1.69E-46
MS.gene050398	MS.gene48195	0.800131	1.66E-48	-1.69E-46
MS.gene050398	MS.gene52065	0.801956	7.05E-49	-1.69E-46
MS.gene050398	MS.gene52399	0.810044	1.40E-50	-1.69E-46
MS.gene050398	MS.gene53556	0.805363	1.38E-49	-1.69E-46
MS.gene050398	MS.gene54020	0.822076	2.87E-53	-1.69E-46
MS.gene050398	MS.gene56057	0.815034	1.14E-51	-1.69E-46
MS.gene050398	MS.gene56177	0.804018	2.64E-49	-1.69E-46
MS.gene050398	MS.gene58166	0.809803	1.58E-50	-1.69E-46
MS.gene050398	MS.gene58674	0.803474	3.43E-49	-1.69E-46
MS.gene050398	MS.gene59149	0.840635	7.72E-58	-1.69E-46
MS.gene050398	MS.gene60124	0.800545	1.37E-48	-1.69E-46
MS.gene050398	MS.gene60286	0.801105	1.05E-48	-1.69E-46
MS.gene050398	MS.gene63882	0.810933	9.01E-51	-1.69E-46
MS.gene050398	MS.gene64049	0.808272	3.36E-50	-1.69E-46
MS.gene050398	MS.gene64994	0.81792	2.57E-52	-1.69E-46
MS.gene050398	MS.gene66292	0.820097	8.21E-53	-1.69E-46
MS.gene050398	MS.gene68819	0.839758	1.31E-57	-1.69E-46
MS.gene050398	MS.gene70307	0.807092	5.99E-50	-1.69E-46
MS.gene050398	MS.gene71353	0.803788	2.95E-49	-1.69E-46
MS.gene050398	MS.gene71450	0.803903	2.79E-49	-1.69E-46
MS.gene050398	MS.gene73745	0.809519	1.82E-50	-1.69E-46
MS.gene050398	MS.gene78405	0.802411	5.68E-49	-1.69E-46
MS.gene050398	MS.gene79683	0.827399	1.60E-54	-1.69E-46
MS.gene050398	MS.gene79842	0.807549	4.79E-50	-1.69E-46
MS.gene050398	MS.gene80153	0.820248	7.58E-53	-1.69E-46
MS.gene050398	MS.gene81388	0.816073	6.67E-52	-1.69E-46
MS.gene050398	MS.gene82533	0.816021	6.85E-52	-1.69E-46
MS.gene050398	MS.gene82939	0.801834	7.47E-49	-1.69E-46
MS.gene050398	MS.gene85860	0.824041	1.00E-53	-1.69E-46
MS.gene050398	MS.gene89092	0.846244	2.45E-59	-1.69E-46
MS.gene050398	MS.gene90770	0.803122	4.05E-49	-1.69E-46
MS.gene050398	MS.gene90771	0.80333	3.67E-49	-1.69E-46
MS.gene050398	MS.gene91509	0.813659	2.29E-51	-1.69E-46
MS.gene050398	MS.gene91845	0.813868	2.06E-51	-1.69E-46
MS.gene050398	MS.gene93892	0.818209	2.21E-52	-1.69E-46
MS.gene050398	MS.gene94333	0.823014	1.74E-53	-1.69E-46
MS.gene050398	MS.gene99375	0.808987	2.36E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050398.t1	MTR_2g038720	92.105	114	9	0	1	114	72	185	2.84e-65	199
MS.gene050398.t1	MTR_4g126350	46.617	133	43	6	5	114	80	207	4.24e-25	96.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050398.t1	AT4G26150	35.455	110	60	3	14	114	113	220	8.62e-13	63.5

Find 24 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAATCATTCTCTTCTTTGA+TGG	0.270861	2.3:+50400717	None:intergenic
TTTCAAACAACAGCACTGTT+AGG	0.320261	2.3:-50400590	MS.gene050398:CDS
CTCTGGAGAAGTGGACCAAC+TGG	0.407869	2.3:-50400534	MS.gene050398:CDS
TTGTACCTTCTAGTGAATCA+TGG	0.424632	2.3:-50400836	MS.gene050398:CDS
TGATCCCATGATTCACTAGA+AGG	0.441692	2.3:+50400831	None:intergenic
CAAAGAAGAGAATGATTAAG+AGG	0.444414	2.3:-50400713	MS.gene050398:CDS
TTCTCAGTTCCTACTAGATC+AGG	0.466474	2.3:+50400630	None:intergenic
GAGAATGATTAAGAGGATGA+TGG	0.470043	2.3:-50400706	MS.gene050398:CDS
TTCAAACAACAGCACTGTTA+GGG	0.471012	2.3:-50400589	MS.gene050398:CDS
GGATCATCAAGAAGAGGAAA+AGG	0.474295	2.3:-50400814	MS.gene050398:CDS
CAAGTATCACCTGATCTAGT+AGG	0.482391	2.3:-50400639	MS.gene050398:CDS
GAGGATGATGGAAGATCAAA+GGG	0.487515	2.3:-50400694	MS.gene050398:CDS
TGTACCTTCTAGTGAATCAT+GGG	0.494972	2.3:-50400835	MS.gene050398:CDS
TTGGTCCACTTCTCCAGAGA+GGG	0.496789	2.3:+50400538	None:intergenic
ACACAACTAAGACCCCTCTC+TGG	0.508622	2.3:-50400551	MS.gene050398:CDS
AGAGGATGATGGAAGATCAA+AGG	0.536944	2.3:-50400695	MS.gene050398:CDS
AGGATAGAACTTCAATGAAG+TGG	0.543169	2.3:-50400743	MS.gene050398:CDS
AAGACCCCTCTCTGGAGAAG+TGG	0.553472	2.3:-50400543	MS.gene050398:CDS
AAGTGGACCAACTGGTCCCA+AGG	0.591915	2.3:-50400526	None:intergenic
AGAGAGGGGTCTTAGTTGTG+TGG	0.598153	2.3:+50400553	None:intergenic
ATATGTACCTTGGGACCAGT+TGG	0.609719	2.3:+50400519	None:intergenic
TGGTCCACTTCTCCAGAGAG+GGG	0.626088	2.3:+50400539	None:intergenic
ATCATGGGATCATCAAGAAG+AGG	0.628177	2.3:-50400820	MS.gene050398:CDS
GTTGGTCCACTTCTCCAGAG+AGG	0.682429	2.3:+50400537	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAGATAATAATGAAAATTTT+AGG	-	chr2.3:50400603-50400622	MS.gene050398:CDS	10.0%
!!!	AGATAATAATGAAAATTTTA+GGG	-	chr2.3:50400604-50400623	MS.gene050398:CDS	10.0%
!	TTAATCATTCTCTTCTTTGA+TGG	+	chr2.3:50400659-50400678	None:intergenic	25.0%
!!!	TAATGAAAATTTTAGGGATG+AGG	-	chr2.3:50400610-50400629	MS.gene050398:CDS	25.0%
	AGGAAAAGGATGAAAAAGAT+TGG	-	chr2.3:50400573-50400592	MS.gene050398:CDS	30.0%
	CAAAGAAGAGAATGATTAAG+AGG	-	chr2.3:50400660-50400679	MS.gene050398:CDS	30.0%
	AGGATAGAACTTCAATGAAG+TGG	-	chr2.3:50400630-50400649	MS.gene050398:CDS	35.0%
	GAGAATGATTAAGAGGATGA+TGG	-	chr2.3:50400667-50400686	MS.gene050398:CDS	35.0%
	TGTACCTTCTAGTGAATCAT+GGG	-	chr2.3:50400538-50400557	MS.gene050398:CDS	35.0%
	TTCAAACAACAGCACTGTTA+GGG	-	chr2.3:50400784-50400803	MS.gene050398:CDS	35.0%
	TTGTACCTTCTAGTGAATCA+TGG	-	chr2.3:50400537-50400556	MS.gene050398:CDS	35.0%
	TTTCAAACAACAGCACTGTT+AGG	-	chr2.3:50400783-50400802	MS.gene050398:CDS	35.0%
	ATCATGGGATCATCAAGAAG+AGG	-	chr2.3:50400553-50400572	MS.gene050398:CDS	40.0%
	CAAGTATCACCTGATCTAGT+AGG	-	chr2.3:50400734-50400753	MS.gene050398:CDS	40.0%
	GGATCATCAAGAAGAGGAAA+AGG	-	chr2.3:50400559-50400578	MS.gene050398:CDS	40.0%
	TGATCCCATGATTCACTAGA+AGG	+	chr2.3:50400545-50400564	None:intergenic	40.0%
	TTCTCAGTTCCTACTAGATC+AGG	+	chr2.3:50400746-50400765	None:intergenic	40.0%
!!	AGAGGATGATGGAAGATCAA+AGG	-	chr2.3:50400678-50400697	MS.gene050398:CDS	40.0%
!!	GAGGATGATGGAAGATCAAA+GGG	-	chr2.3:50400679-50400698	MS.gene050398:CDS	40.0%
	ACACAACTAAGACCCCTCTC+TGG	-	chr2.3:50400822-50400841	MS.gene050398:CDS	50.0%
	AGAGAGGGGTCTTAGTTGTG+TGG	+	chr2.3:50400823-50400842	None:intergenic	50.0%
	TTGGTCCACTTCTCCAGAGA+GGG	+	chr2.3:50400838-50400857	None:intergenic	50.0%
	AAGTGGACCAACTGGTCCCA+AGG	-	chr2.3:50400847-50400866	MS.gene050398:CDS	55.0%
	GTTGGTCCACTTCTCCAGAG+AGG	+	chr2.3:50400839-50400858	None:intergenic	55.0%
	TGGTCCACTTCTCCAGAGAG+GGG	+	chr2.3:50400837-50400856	None:intergenic	55.0%
!	AAGACCCCTCTCTGGAGAAG+TGG	-	chr2.3:50400830-50400849	MS.gene050398:CDS	55.0%
!	CTCTGGAGAAGTGGACCAAC+TGG	-	chr2.3:50400839-50400858	MS.gene050398:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	50400527	50400868	50400527	ID=MS.gene050398
chr2.3	mRNA	50400527	50400868	50400527	ID=MS.gene050398.t1;Parent=MS.gene050398
chr2.3	exon	50400527	50400868	50400527	ID=MS.gene050398.t1.exon1;Parent=MS.gene050398.t1
chr2.3	CDS	50400527	50400868	50400527	ID=cds.MS.gene050398.t1;Parent=MS.gene050398.t1

Gene Sequence	>MS.gene050398 GTTAAGAATTTTGTACCTTCTAGTGAATCATGGGATCATCAAGAAGAGGAAAAGGATGAAAAAGATTGGAAGAAAGAAGATAATAATGAAAATTTTAGGGATGAGGATAGAACTTCAATGAAGTGGATGCCATCAAAGAAGAGAATGATTAAGAGGATGATGGAAGATCAAAGGGCTTCTGAGCAAGAGTTTGAGAAACAAATTAAGCAAGTATCACCTGATCTAGTAGGAACTGAGAATAGTAGCAACAACAATTTTTCAAACAACAGCACTGTTAGGGTTTGTACTGACTGCCACACAACTAAGACCCCTCTCTGGAGAAGTGGACCAACTGGTCCCAAG
Protein sequence	>MS.gene050398.t1 VKNFVPSSESWDHQEEEKDEKDWKKEDNNENFRDEDRTSMKWMPSKKRMIKRMMEDQRASEQEFEKQIKQVSPDLVGTENSSNNNFSNNSTVRVCTDCHTTKTPLWRSGPTGPK