AlfalfaGEDB | MS.gene32854

M. sativa cultivar XinJiangDaYe / MS.gene32854

KEGG: K09285
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00847.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32854.t1	XP_024635770.1	94.2	622	28	2	21	642	48	661	0.00E+00	1140.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32854.t1	Q38914	54.7	464	121	11	37	473	65	466	4.8e-117	423.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32854.t1	A0A072VCJ1	93.7	622	28	3	21	642	48	658	0.0e+00	1129.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene32854.t1	TF	AP2/ERF-AP2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049418	MS.gene32854	0.847372	1.21E-59	-1.69E-46
MS.gene049660	MS.gene32854	0.804747	1.86E-49	-1.69E-46
MS.gene051067	MS.gene32854	0.811405	7.12E-51	-1.69E-46
MS.gene051648	MS.gene32854	0.881282	2.73E-70	-1.69E-46
MS.gene051660	MS.gene32854	0.860239	2.37E-63	-1.69E-46
MS.gene051664	MS.gene32854	0.810555	1.09E-50	-1.69E-46
MS.gene052150	MS.gene32854	0.83229	1.03E-55	-1.69E-46
MS.gene052151	MS.gene32854	0.842096	3.19E-58	-1.69E-46
MS.gene053144	MS.gene32854	0.820544	6.49E-53	-1.69E-46
MS.gene053536	MS.gene32854	0.889068	3.37E-73	-1.69E-46
MS.gene053538	MS.gene32854	0.888833	4.16E-73	-1.69E-46
MS.gene053540	MS.gene32854	0.80635	8.59E-50	-1.69E-46
MS.gene053945	MS.gene32854	0.830952	2.20E-55	-1.69E-46
MS.gene054557	MS.gene32854	0.800471	1.42E-48	-1.69E-46
MS.gene054584	MS.gene32854	0.824233	9.02E-54	-1.69E-46
MS.gene054999	MS.gene32854	0.824653	7.18E-54	-1.69E-46
MS.gene055537	MS.gene32854	0.830989	2.15E-55	-1.69E-46
MS.gene056169	MS.gene32854	0.835868	1.31E-56	-1.69E-46
MS.gene056228	MS.gene32854	0.804761	1.85E-49	-1.69E-46
MS.gene057082	MS.gene32854	0.819175	1.33E-52	-1.69E-46
MS.gene058197	MS.gene32854	0.804045	2.61E-49	-1.69E-46
MS.gene058198	MS.gene32854	0.800077	1.71E-48	-1.69E-46
MS.gene058386	MS.gene32854	0.812386	4.35E-51	-1.69E-46
MS.gene058668	MS.gene32854	0.805075	1.59E-49	-1.69E-46
MS.gene058823	MS.gene32854	0.800027	1.75E-48	-1.69E-46
MS.gene05894	MS.gene32854	0.849032	4.20E-60	-1.69E-46
MS.gene059300	MS.gene32854	0.818621	1.78E-52	-1.69E-46
MS.gene059424	MS.gene32854	0.882877	7.21E-71	-1.69E-46
MS.gene059425	MS.gene32854	0.816101	6.58E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32854.t1	MTR_3g103460	90.597	670	23	6	1	642	1	658	0.0	1190
MS.gene32854.t1	MTR_1g017400	60.142	705	171	25	1	641	1	659	0.0	714
MS.gene32854.t1	MTR_4g097520	50.578	692	253	19	1	641	1	654	1.12e-176	517
MS.gene32854.t1	MTR_8g020510	51.142	438	155	11	53	479	49	438	4.79e-131	397
MS.gene32854.t1	MTR_5g015070	50.108	463	158	9	25	478	42	440	3.60e-127	386
MS.gene32854.t1	MTR_7g080460	78.774	212	44	1	276	487	248	458	2.46e-115	360
MS.gene32854.t1	MTR_4g065370	68.000	250	61	3	228	476	122	353	6.84e-115	355
MS.gene32854.t1	MTR_4g127930	63.433	268	86	2	213	468	73	340	8.22e-115	353
MS.gene32854.t1	MTR_2g098180	65.600	250	64	2	228	476	129	357	1.74e-111	345
MS.gene32854.t1	MTR_8g068510	66.516	221	64	3	280	494	124	340	3.85e-98	306
MS.gene32854.t1	MTR_5g031880	76.768	198	42	2	278	475	215	408	1.56e-95	303
MS.gene32854.t1	MTR_4g130270	71.598	169	48	0	286	454	46	214	3.77e-84	268
MS.gene32854.t1	MTR_2g460730	50.709	282	109	7	254	519	22	289	8.69e-76	247
MS.gene32854.t1	MTR_7g009410	62.234	188	67	2	267	454	46	229	2.58e-75	246
MS.gene32854.t1	MTR_6g011490	59.903	207	72	3	248	454	30	225	2.52e-74	243
MS.gene32854.t1	MTR_8g044070	58.654	208	76	3	254	454	15	219	6.63e-73	241
MS.gene32854.t1	MTR_8g044070	37.113	97	53	1	278	374	145	233	4.18e-11	65.9
MS.gene32854.t1	MTR_8g468920	63.736	182	63	1	273	454	55	233	1.98e-70	234
MS.gene32854.t1	MTR_4g007770	68.047	169	51	1	286	454	35	200	1.98e-70	231
MS.gene32854.t1	MTR_8g044040	58.586	198	70	3	286	483	55	240	1.38e-67	226
MS.gene32854.t1	MTR_4g094868	55.357	168	64	3	290	457	176	332	2.45e-45	169
MS.gene32854.t1	MTR_4g094868	55.357	168	64	3	290	457	176	332	2.54e-45	169
MS.gene32854.t1	MTR_5g016810	55.357	168	64	3	290	457	152	308	7.77e-45	167
MS.gene32854.t1	MTR_2g093060	45.652	230	98	7	244	457	72	290	6.02e-44	164
MS.gene32854.t1	MTR_2g093060	45.652	230	98	7	244	457	72	290	6.99e-44	164
MS.gene32854.t1	MTR_4g061200	52.381	168	69	3	290	457	131	287	9.99e-44	161
MS.gene32854.t1	MTR_7g100590	52.381	168	69	3	290	457	139	295	6.65e-43	161
MS.gene32854.t1	MTR_4g061200	52.381	168	69	3	290	457	131	287	1.01e-42	160
MS.gene32854.t1	MTR_5g016810	52.874	174	65	4	290	457	152	314	4.28e-42	160
MS.gene32854.t1	MTR_7g091390	57.237	152	54	3	263	414	36	176	1.25e-40	153
MS.gene32854.t1	MTR_4g094868	56.934	137	58	1	321	457	41	176	1.31e-37	144
MS.gene32854.t1	MTR_2g093060	42.609	230	90	8	244	457	72	275	1.43e-35	140
MS.gene32854.t1	MTR_4g061200	48.214	168	61	4	290	457	131	272	1.79e-35	138
MS.gene32854.t1	MTR_4g061200	48.214	168	61	4	290	457	131	272	1.55e-34	137
MS.gene32854.t1	MTR_7g061190	50.735	136	66	1	322	457	3	137	1.37e-30	117
MS.gene32854.t1	MTR_7g091390	63.736	91	28	1	324	414	3	88	4.05e-24	104
MS.gene32854.t1	MTR_1g049140	42.361	144	72	3	266	409	113	245	6.05e-21	93.2
MS.gene32854.t1	MTR_1g049140	42.361	144	72	3	266	409	113	245	1.40e-20	94.7
MS.gene32854.t1	MTR_1g049140	42.361	144	72	3	266	409	113	245	4.65e-20	92.4
MS.gene32854.t1	MTR_3g090510	47.170	106	55	1	328	433	163	267	4.38e-18	87.4
MS.gene32854.t1	MTR_7g060560	44.340	106	57	2	328	433	156	259	5.69e-15	77.8
MS.gene32854.t1	MTR_1g090290	41.573	89	42	3	270	358	94	172	1.49e-11	64.3
MS.gene32854.t1	MTR_1g028560	49.315	73	36	1	377	449	99	170	3.41e-11	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32854.t1	AT4G37750	56.250	464	114	10	37	473	65	466	3.25e-158	466
MS.gene32854.t1	AT1G72570	71.605	243	67	2	231	472	164	405	3.01e-122	369
MS.gene32854.t1	AT1G51190	68.651	252	73	3	228	476	129	377	1.08e-117	363
MS.gene32854.t1	AT3G20840	69.200	250	69	4	228	476	126	368	1.37e-115	357
MS.gene32854.t1	AT5G57390	78.894	199	42	0	270	468	65	263	1.78e-115	352
MS.gene32854.t1	AT5G57390	78.894	199	42	0	270	468	183	381	1.76e-114	354
MS.gene32854.t1	AT5G17430	68.145	248	71	2	278	522	136	378	6.34e-112	346
MS.gene32854.t1	AT5G17430	68.980	245	74	1	278	522	198	440	1.92e-111	347
MS.gene32854.t1	AT5G65510	69.302	215	66	0	263	477	146	360	1.88e-95	301
MS.gene32854.t1	AT5G65510	69.302	215	66	0	263	477	204	418	2.70e-95	302
MS.gene32854.t1	AT5G65510	69.302	215	66	0	263	477	204	418	2.70e-95	302
MS.gene32854.t1	AT5G10510	76.263	198	46	1	278	475	218	414	4.46e-95	303
MS.gene32854.t1	AT5G10510	76.263	198	46	1	278	475	218	414	4.46e-95	303
MS.gene32854.t1	AT5G10510	76.263	198	46	1	278	475	241	437	7.22e-95	303
MS.gene32854.t1	AT5G10510	76.263	198	46	1	278	475	256	452	7.34e-95	304
MS.gene32854.t1	AT5G10510	76.263	198	46	1	278	475	256	452	7.34e-95	304
MS.gene32854.t1	AT5G10510	74.747	198	46	2	278	475	218	411	1.33e-90	291
MS.gene32854.t1	AT5G10510	67.890	218	49	2	278	475	256	472	1.35e-87	285
MS.gene32854.t1	AT5G65510	72.671	161	44	0	263	423	146	306	2.69e-82	262
MS.gene32854.t1	AT5G65510	42.667	75	35	1	391	457	172	246	6.10e-11	64.7
MS.gene32854.t1	AT3G54320	68.966	174	53	1	286	459	61	233	1.23e-73	243
MS.gene32854.t1	AT3G54320	68.966	174	53	1	286	459	61	233	2.05e-73	243
MS.gene32854.t1	AT1G79700	69.822	169	51	0	286	454	48	216	4.53e-68	225
MS.gene32854.t1	AT1G16060	56.277	231	96	1	246	471	14	244	5.43e-67	223
MS.gene32854.t1	AT1G79700	65.680	169	48	1	286	454	48	206	3.26e-60	204
MS.gene32854.t1	AT3G54320	69.853	136	40	1	324	459	33	167	4.69e-54	189
MS.gene32854.t1	AT3G54320	69.853	136	40	1	324	459	33	167	4.69e-54	189
MS.gene32854.t1	AT1G79700	68.702	131	41	0	324	454	10	140	4.36e-46	163
MS.gene32854.t1	AT4G36920	50.739	203	88	4	255	457	97	287	4.94e-46	169
MS.gene32854.t1	AT4G36920	50.739	203	88	4	255	457	97	287	4.94e-46	169
MS.gene32854.t1	AT5G67180	44.304	237	111	4	234	470	50	265	1.28e-45	164
MS.gene32854.t1	AT1G16060	68.421	133	42	0	322	454	20	152	4.08e-45	162
MS.gene32854.t1	AT5G67180	44.304	237	111	4	234	470	50	265	7.18e-45	164
MS.gene32854.t1	AT5G67180	44.206	233	109	4	234	466	50	261	8.45e-45	161
MS.gene32854.t1	AT2G41710	58.553	152	55	2	263	414	46	189	9.60e-45	165
MS.gene32854.t1	AT2G41710	58.553	152	55	2	263	414	46	189	1.66e-44	165
MS.gene32854.t1	AT1G79700	67.442	129	42	0	326	454	6	134	3.61e-44	158
MS.gene32854.t1	AT2G41710	56.688	157	55	3	263	414	46	194	2.26e-42	159
MS.gene32854.t1	AT2G28550	51.786	168	70	3	290	457	153	309	8.66e-42	158
MS.gene32854.t1	AT2G41710	47.594	187	55	3	263	414	46	224	4.57e-38	147
MS.gene32854.t1	AT2G28550	51.064	141	58	3	290	430	153	282	2.29e-34	134
MS.gene32854.t1	AT5G67180	40.265	226	113	5	234	459	50	253	5.80e-34	130
MS.gene32854.t1	AT2G28550	47.619	168	62	4	290	457	153	294	1.19e-33	134
MS.gene32854.t1	AT3G54320	70.968	93	26	1	367	459	1	92	7.66e-33	128
MS.gene32854.t1	AT1G16060	60.909	110	38	1	367	471	1	110	7.22e-32	123
MS.gene32854.t1	AT1G16060	60.909	110	38	1	367	471	1	110	7.22e-32	123
MS.gene32854.t1	AT1G16060	60.909	110	38	1	367	471	1	110	7.22e-32	123
MS.gene32854.t1	AT1G16060	60.909	110	38	1	367	471	1	110	7.22e-32	123
MS.gene32854.t1	AT2G28550	51.181	127	51	3	290	416	153	268	5.09e-30	119
MS.gene32854.t1	AT2G28550	51.181	127	51	3	290	416	153	268	5.09e-30	119
MS.gene32854.t1	AT2G39250	44.643	112	52	2	291	402	109	210	3.35e-26	107
MS.gene32854.t1	AT2G39250	44.248	113	53	2	290	402	108	210	9.84e-26	108
MS.gene32854.t1	AT2G39250	29.368	269	150	12	372	633	88	323	4.26e-11	65.1
MS.gene32854.t1	AT2G28550	45.522	134	57	2	324	457	11	128	2.02e-23	101
MS.gene32854.t1	AT5G60120	40.805	174	76	6	274	447	145	291	8.57e-23	102
MS.gene32854.t1	AT5G60120	47.287	129	57	3	287	415	157	274	9.32e-23	101
MS.gene32854.t1	AT5G60120	47.287	129	57	3	287	415	157	274	9.32e-23	101
MS.gene32854.t1	AT5G60120	47.287	129	57	3	287	415	157	274	9.32e-23	101
MS.gene32854.t1	AT5G60120	40.805	174	76	6	274	447	145	291	9.61e-23	102
MS.gene32854.t1	AT5G60120	40.805	174	76	6	274	447	145	291	1.69e-22	102
MS.gene32854.t1	AT3G54990	48.598	107	45	2	290	396	122	218	5.20e-21	93.2
MS.gene32854.t1	AT3G54990	48.598	107	45	2	290	396	122	218	1.71e-20	94.0
MS.gene32854.t1	AT1G16060	62.025	79	25	1	398	471	10	88	4.54e-17	80.1

Find 125 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATGCATGTTACAGTATATT+TGG	0.085782	3.1:+81908332	MS.gene32854:intron
TTGGATGATTCTACCAAAAT+AGG	0.146027	3.1:+81909802	MS.gene32854:CDS
GCTAGCAATACTCTTCTTTC+TGG	0.154896	3.1:+81909511	MS.gene32854:CDS
CTAATGTTGCTGTTAGTTCT+TGG	0.205616	3.1:+81909947	MS.gene32854:CDS
AGGAAATCTATTGACACTTT+TGG	0.225909	3.1:+81907906	MS.gene32854:CDS
GTAGATATGAAGCACATTTA+TGG	0.228782	3.1:+81908148	MS.gene32854:CDS
CCTCTAATGGTGCTTGAAAT+AGG	0.284929	3.1:-81907557	None:intergenic
AAGCCATTGCCTCACTTTCA+TGG	0.308137	3.1:-81907392	None:intergenic
CTGCAAAACCATCTTCCATT+TGG	0.319566	3.1:-81909665	None:intergenic
TGTTATGGTGTTCCTGAAAA+TGG	0.323560	3.1:+81907013	MS.gene32854:CDS
AACTCAAGGTGGTTATGATA+TGG	0.323727	3.1:+81908469	MS.gene32854:intron
TCTTTAGATTCTACCTCTAA+TGG	0.324936	3.1:-81907570	None:intergenic
CCAAGAAAATCCTCTAGTTT+AGG	0.328835	3.1:-81907336	None:intergenic
TTTGATTCCATTGAAGGCTT+TGG	0.329887	3.1:-81909916	None:intergenic
TATTCTCCATTAGGCATAGA+TGG	0.336451	3.1:+81908121	MS.gene32854:intron
TAACCAAGTGCTGCATAATA+TGG	0.342134	3.1:-81907528	None:intergenic
TCATTTCCTCAAGTTGAGTA+TGG	0.344608	3.1:-81908672	None:intergenic
ATAGGCATAACAGTCAAATT+AGG	0.347664	3.1:-81907052	None:intergenic
TCAAGCAGCTCTTAAGTATT+GGG	0.347974	3.1:+81908517	MS.gene32854:CDS
GGTGGTTCTTCTTCTGTTGT+TGG	0.362895	3.1:+81907714	MS.gene32854:CDS
CCTATTTCAAGCACCATTAG+AGG	0.368785	3.1:+81907557	MS.gene32854:CDS
ATGCATGTTACAGTATATTT+GGG	0.371710	3.1:+81908333	MS.gene32854:intron
CCTAGACAGATCTCACCTTC+TGG	0.379310	3.1:+81907813	MS.gene32854:CDS
CGCTTCAGCAGCTTCTCCAT+TGG	0.384097	3.1:-81909617	None:intergenic
AGAGTTACTACTGTCATTCA+TGG	0.391896	3.1:-81906819	None:intergenic
CAGCAGCTTCTCCATTGGTC+TGG	0.395700	3.1:-81909612	None:intergenic
GGTAACAAGGACCTTTATCT+TGG	0.399524	3.1:+81909020	MS.gene32854:CDS
GTTCCCAAATGCCTCAAAAC+TGG	0.400777	3.1:+81907619	MS.gene32854:CDS
AATCATAACAACAATTGGTT+AGG	0.403766	3.1:+81906851	MS.gene32854:CDS
TAGATATGAAGCACATTTAT+GGG	0.403876	3.1:+81908149	MS.gene32854:CDS
TGGTATTAATTCAGTTGGTT+TGG	0.404298	3.1:+81909783	MS.gene32854:CDS
GCAAGGATTGGAAGAGTTGC+TGG	0.408354	3.1:+81908999	MS.gene32854:CDS
ACTCTTTCCAAAGCCTTCAA+TGG	0.408843	3.1:+81909909	MS.gene32854:CDS
TGGAACAGAATCAGTGACTA+TGG	0.411439	3.1:+81907833	MS.gene32854:CDS
ACAACAATCATAACAACAAT+TGG	0.411645	3.1:+81906846	MS.gene32854:CDS
TACTGAATGTTCCAAGATAA+AGG	0.412580	3.1:-81909031	None:intergenic
TCTTCTGTTGTTGGTACTGT+AGG	0.414963	3.1:+81907723	MS.gene32854:CDS
TGGATGATTCTACCAAAATA+GGG	0.417120	3.1:+81909803	MS.gene32854:CDS
AAGGAAGGACAAACAAGAAA+AGG	0.424488	3.1:+81908186	MS.gene32854:CDS
TTCAACACACATAAACTTCC+CGG	0.425153	3.1:+81908544	MS.gene32854:CDS
TATGCAGCACTTGGTTATCA+TGG	0.425353	3.1:+81907534	MS.gene32854:CDS
ACAAACTCAACAATCTGTTA+TGG	0.433973	3.1:+81906998	MS.gene32854:CDS
ACTAGCATGGGGAGTCATAA+TGG	0.434049	3.1:+81907681	MS.gene32854:CDS
TCAATAGATTTCCTATGAAT+AGG	0.438789	3.1:-81907897	None:intergenic
ATCAAGCAGCTCTTAAGTAT+TGG	0.446724	3.1:+81908516	MS.gene32854:CDS
TTCTTTCTGGTCAGCATGCA+AGG	0.453728	3.1:+81909524	MS.gene32854:CDS
AGTGATCCATCTGACTTGAT+AGG	0.454921	3.1:-81907070	None:intergenic
AAACTTGTCACCAATGATGA+TGG	0.469910	3.1:-81909841	None:intergenic
TAATGATTCCAAATGGAAGA+TGG	0.471281	3.1:+81909657	MS.gene32854:CDS
ATGTCTTTACAAGACCTTGT+TGG	0.474882	3.1:+81909763	MS.gene32854:CDS
AAAATCATTGTGAATTCTGA+TGG	0.478976	3.1:+81909715	MS.gene32854:CDS
CTTTGTATCATGGAAGCTCT+TGG	0.479184	3.1:+81907091	MS.gene32854:CDS
GGGAAGTTTATGTGTGTTGA+AGG	0.488674	3.1:-81908542	None:intergenic
CTTGTTGGTATTAATTCAGT+TGG	0.489454	3.1:+81909778	MS.gene32854:CDS
AGAACTAACAGCAACATTAG+TGG	0.490687	3.1:-81909944	None:intergenic
ACGAAAATAATGATTCCAAA+TGG	0.491698	3.1:+81909650	MS.gene32854:CDS
GTCAAAAGCAGCCTATTCAT+AGG	0.492472	3.1:+81907886	MS.gene32854:CDS
GGTAGGTGGCAAGCAAGGAT+TGG	0.507927	3.1:+81908987	MS.gene32854:CDS
CATCCATATTATGCAGCACT+TGG	0.508376	3.1:+81907525	MS.gene32854:CDS
TTTCTTGGTGGTGCAACAAT+GGG	0.508995	3.1:+81907351	MS.gene32854:CDS
AGATGGATCACTTTGTATCA+TGG	0.512780	3.1:+81907081	MS.gene32854:CDS
ACACAGCTAGATTGAGAACC+AGG	0.513570	3.1:-81907783	None:intergenic
AGTCATAATGGTGGAGCTGG+TGG	0.513615	3.1:+81907693	MS.gene32854:CDS
TCCATTAGGCATAGATGGAC+TGG	0.517509	3.1:+81908126	MS.gene32854:intron
ATATGATGTTGAAAGGATCA+TGG	0.520797	3.1:+81909489	MS.gene32854:CDS
CATAATGGTGGAGCTGGTGG+TGG	0.521042	3.1:+81907696	MS.gene32854:CDS
GGGAGTCATAATGGTGGAGC+TGG	0.523283	3.1:+81907690	MS.gene32854:CDS
GTCTGAAAGATTCTGAAAGA+AGG	0.527254	3.1:-81907491	None:intergenic
GGCTTTGGAAAGAGTAGTGA+GGG	0.530598	3.1:-81909901	None:intergenic
AAAAGAGGAGCTGCTAAGCT+TGG	0.531048	3.1:+81907864	MS.gene32854:CDS
GAGAACCAGGGCTCATTGAA+AGG	0.533035	3.1:-81907770	None:intergenic
CCAGAAGGTGAGATCTGTCT+AGG	0.534044	3.1:-81907813	None:intergenic
TATCTAAATATGATGTTGAA+AGG	0.534738	3.1:+81909482	MS.gene32854:CDS
TATTCATGAACTTCGGTCTG+TGG	0.536183	3.1:-81909565	None:intergenic
GACAAATTGATTGAAGATGC+TGG	0.538955	3.1:-81910000	None:intergenic
TTAAGCCTTTCAATGAGCCC+TGG	0.541546	3.1:+81907765	MS.gene32854:CDS
GTTGAGTTTGTTAAGTGAGA+AGG	0.542491	3.1:-81906986	None:intergenic
CTGAAAGAAGGTCAAGGGAA+TGG	0.549375	3.1:-81907479	None:intergenic
ATGGGATAACAGTTGCAAGA+AGG	0.549719	3.1:+81908167	MS.gene32854:CDS
ACAATGGGTAGTGATGAATA+TGG	0.550458	3.1:+81907366	MS.gene32854:CDS
CAGAGAACATCTCAGTATAG+AGG	0.550832	3.1:+81907930	MS.gene32854:CDS
TGCAAGGAGAATCAAAGATA+AGG	0.550976	3.1:+81909540	MS.gene32854:CDS
ATTTGTGTAGCTACTTGTGA+AGG	0.555126	3.1:-81909973	None:intergenic
TTGCTTGCCACCTACCATGT+TGG	0.556430	3.1:-81908980	None:intergenic
ATGATTATATTCATGAACTT+CGG	0.556488	3.1:-81909572	None:intergenic
AATGAAGAATATGACAAGGC+AGG	0.556697	3.1:+81908690	MS.gene32854:CDS
GAAAGAAGCAGGAACAGTGT+TGG	0.556882	3.1:-81906954	None:intergenic
CAGATGAACACTACTAGCAT+GGG	0.557648	3.1:+81907669	MS.gene32854:CDS
TGGAAGAGTTGCTGGTAACA+AGG	0.559839	3.1:+81909007	MS.gene32854:CDS
GCAGATGAACACTACTAGCA+TGG	0.560674	3.1:+81907668	MS.gene32854:CDS
TTGTAACAGCATTTGCTCCA+CGG	0.565794	3.1:-81909450	None:intergenic
AGGCTTTGGAAAGAGTAGTG+AGG	0.568883	3.1:-81909902	None:intergenic
AAGATTCTGAAAGAAGGTCA+AGG	0.578912	3.1:-81907485	None:intergenic
CACAAGTAGCTACACAAATG+AGG	0.580768	3.1:+81909977	MS.gene32854:CDS
GTTATGCCTATCAAGTCAGA+TGG	0.582455	3.1:+81907064	MS.gene32854:CDS
AATATGTTGCACATTTGAGA+AGG	0.587308	3.1:+81908713	MS.gene32854:CDS
TAGCCATGAAAGTGAGGCAA+TGG	0.587506	3.1:+81907389	MS.gene32854:CDS
GTTGTTGGTACTGTAGGAAG+TGG	0.597270	3.1:+81907729	MS.gene32854:CDS
AAACTACCATACTCAACTTG+AGG	0.599024	3.1:+81908666	MS.gene32854:CDS
ATCTTCATCTCCTAAACTAG+AGG	0.599319	3.1:+81907326	MS.gene32854:CDS
AACATGGTAGGTGGCAAGCA+AGG	0.600209	3.1:+81908982	MS.gene32854:CDS
CAATGTACAGAGGCGTGACA+AGG	0.600518	3.1:+81908860	MS.gene32854:CDS
GTCACTGATTCTGTTCCAGA+AGG	0.600695	3.1:-81907828	None:intergenic
GCAGACATCACCAACATGGT+AGG	0.604873	3.1:+81908970	MS.gene32854:intron
AGATTCTGAAAGAAGGTCAA+GGG	0.611616	3.1:-81907484	None:intergenic
CTCAGTATAGAGGTGTCACA+AGG	0.617014	3.1:+81907940	MS.gene32854:CDS
CAAGCAGCTCTTAAGTATTG+GGG	0.623947	3.1:+81908518	MS.gene32854:CDS
CAAACAAGAAAAGGAAGACA+AGG	0.624646	3.1:+81908195	MS.gene32854:CDS
AATGTATCGAGCCAGACCAA+TGG	0.631591	3.1:+81909601	MS.gene32854:CDS
TCTAGTTTAGGAGATGAAGA+TGG	0.633764	3.1:-81907324	None:intergenic
GACATCACCAACATGGTAGG+TGG	0.639099	3.1:+81908973	MS.gene32854:intron
TGGTCTGTATTAGCTTGATG+AGG	0.640420	3.1:-81907459	None:intergenic
GATAACAGTTGCAAGAAGGA+AGG	0.640507	3.1:+81908171	MS.gene32854:CDS
AAAAGCAGTGGATTCTCAAG+GGG	0.641907	3.1:+81908832	MS.gene32854:CDS
ATATGGTAGCCATGAAAGTG+AGG	0.643799	3.1:+81907383	MS.gene32854:CDS
CTTGGTAGATCACAATCACA+AGG	0.655160	3.1:+81907109	MS.gene32854:CDS
CAACTGAATTAATACCAACA+AGG	0.665562	3.1:-81909777	None:intergenic
GTAGCAGCAATAAAATTCCG+TGG	0.666673	3.1:+81909433	MS.gene32854:CDS
AACAGAATCAGTGACTATGG+AGG	0.680741	3.1:+81907836	MS.gene32854:CDS
AGCATGGGGAGTCATAATGG+TGG	0.689715	3.1:+81907684	MS.gene32854:CDS
AGGAAATGAAGAATATGACA+AGG	0.693051	3.1:+81908686	MS.gene32854:CDS
GGGAACTAGAAACATCAACA+TGG	0.697253	3.1:-81907602	None:intergenic
CACAGCTAGATTGAGAACCA+GGG	0.711589	3.1:-81907782	None:intergenic
AGATGAACACTACTAGCATG+GGG	0.712889	3.1:+81907670	MS.gene32854:CDS
AGGGGTGCATCAATGTACAG+AGG	0.720794	3.1:+81908850	MS.gene32854:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAGAAAAAAAATTACAAAA+AGG	-	chr3.1:81909362-81909381	None:intergenic	10.0%
!!!	TAAAAATAACAACTTTTTTT+TGG	-	chr3.1:81907221-81907240	None:intergenic	10.0%
!!	ACAAATTCAAATGAAAAAAA+AGG	-	chr3.1:81908905-81908924	None:intergenic	15.0%
!!	CAAATTCAAATGAAAAAAAA+GGG	-	chr3.1:81908904-81908923	None:intergenic	15.0%
!!	TCTTTAATTAATGTTAGATA+TGG	+	chr3.1:81907243-81907262	MS.gene32854:intron	15.0%
!!!	TGTTAGTATAAAATTTTAGT+AGG	-	chr3.1:81908266-81908285	None:intergenic	15.0%
!!!	TTGTAATTTTTTTTCTTTCT+TGG	+	chr3.1:81909364-81909383	MS.gene32854:intron	15.0%
!!	AAAACAACATAATCATTACA+GGG	-	chr3.1:81907269-81907288	None:intergenic	20.0%
!!	AAGCATTTAAATTATGAACA+AGG	+	chr3.1:81909102-81909121	MS.gene32854:intron	20.0%
!!	AGCATTTAAATTATGAACAA+GGG	+	chr3.1:81909103-81909122	MS.gene32854:intron	20.0%
!!	AGTAAATTAAATTCCTATGA+AGG	-	chr3.1:81909173-81909192	None:intergenic	20.0%
!!	ATGATTATATTCATGAACTT+CGG	-	chr3.1:81909575-81909594	None:intergenic	20.0%
!!	TAATTATATGACAATCATGA+TGG	+	chr3.1:81908784-81908803	MS.gene32854:intron	20.0%
!!	TATCTAAATATGATGTTGAA+AGG	+	chr3.1:81909482-81909501	MS.gene32854:CDS	20.0%
!!	TTATTATATGATTGAAGTGA+TGG	+	chr3.1:81907299-81907318	MS.gene32854:intron	20.0%
!!!	AAATAACAACTTTTTTTTGG+TGG	-	chr3.1:81907218-81907237	None:intergenic	20.0%
!!!	GCAATTATTTTGTAGTAAAA+AGG	+	chr3.1:81908755-81908774	MS.gene32854:intron	20.0%
!!!	TGTTTGTTTTTTCTTTTACT+TGG	-	chr3.1:81908381-81908400	None:intergenic	20.0%
!!!	TTTAAAGTAAATTCCTTCAT+AGG	+	chr3.1:81909157-81909176	MS.gene32854:intron	20.0%
!!!	TTTCTTGGAAATGAAATTTT+AGG	+	chr3.1:81909379-81909398	MS.gene32854:intron	20.0%
!	AAAATCATTGTGAATTCTGA+TGG	+	chr3.1:81909715-81909734	MS.gene32854:CDS	25.0%
!	AATCATAACAACAATTGGTT+AGG	+	chr3.1:81906851-81906870	MS.gene32854:CDS	25.0%
!	ACAACAATCATAACAACAAT+TGG	+	chr3.1:81906846-81906865	MS.gene32854:CDS	25.0%
!	ACGAAAATAATGATTCCAAA+TGG	+	chr3.1:81909650-81909669	MS.gene32854:CDS	25.0%
!	AGGTACATAAATCTGTTAAT+TGG	+	chr3.1:81908733-81908752	MS.gene32854:intron	25.0%
!	ATGCATGTTACAGTATATTT+GGG	+	chr3.1:81908333-81908352	MS.gene32854:intron	25.0%
!	GAAAACAACATAATCATTAC+AGG	-	chr3.1:81907270-81907289	None:intergenic	25.0%
!	TAGATATGAAGCACATTTAT+GGG	+	chr3.1:81908149-81908168	MS.gene32854:CDS	25.0%
!	TCAATAGATTTCCTATGAAT+AGG	-	chr3.1:81907900-81907919	None:intergenic	25.0%
!!!	ACAAAATTCCACTTTTTCTA+AGG	+	chr3.1:81909281-81909300	MS.gene32854:intron	25.0%
!!!	ATTTTTAGTAACAATGTTGG+AGG	-	chr3.1:81909065-81909084	None:intergenic	25.0%
!!!	CACATTTTTAGTAACAATGT+TGG	-	chr3.1:81909068-81909087	None:intergenic	25.0%
!!!	GATTTTGTTTATTCTCCATT+AGG	+	chr3.1:81908112-81908131	MS.gene32854:intron	25.0%
!!!	GGTTTTATTTTTTGAACTCA+AGG	+	chr3.1:81908455-81908474	MS.gene32854:intron	25.0%
!!!	GTTTCTAATTTTGACTTTGT+TGG	+	chr3.1:81908434-81908453	MS.gene32854:intron	25.0%
!!!	TGATTGGTTATGCTTTTATA+AGG	+	chr3.1:81909235-81909254	MS.gene32854:intron	25.0%
!!!	TTTATTTTTTGAACTCAAGG+TGG	+	chr3.1:81908458-81908477	MS.gene32854:intron	25.0%
!!!	TTTTTCAAATCCATCATCAT+TGG	+	chr3.1:81909831-81909850	MS.gene32854:CDS	25.0%
!!!	TTTTTTCTTTTACTTGGTGA+AGG	-	chr3.1:81908375-81908394	None:intergenic	25.0%
	AAAGGGAATCTCTAATAGAA+TGG	+	chr3.1:81909308-81909327	MS.gene32854:intron	30.0%
	AAGCTAACAAGAAAATGCTA+AGG	-	chr3.1:81908582-81908601	None:intergenic	30.0%
	AATATGTTGCACATTTGAGA+AGG	+	chr3.1:81908713-81908732	MS.gene32854:CDS	30.0%
	ACAAACTCAACAATCTGTTA+TGG	+	chr3.1:81906998-81907017	MS.gene32854:CDS	30.0%
	AGAAACAGTAATAGCAATGA+TGG	-	chr3.1:81908420-81908439	None:intergenic	30.0%
	AGGAAATGAAGAATATGACA+AGG	+	chr3.1:81908686-81908705	MS.gene32854:CDS	30.0%
	ATAACCAATCACTTGTGTTT+GGG	-	chr3.1:81909226-81909245	None:intergenic	30.0%
	ATAGGCATAACAGTCAAATT+AGG	-	chr3.1:81907055-81907074	None:intergenic	30.0%
	ATATGATGTTGAAAGGATCA+TGG	+	chr3.1:81909489-81909508	MS.gene32854:CDS	30.0%
	ATCTCTAATAGAATGGTGTA+AGG	+	chr3.1:81909315-81909334	MS.gene32854:intron	30.0%
	ATTTGAATTTGTCACACTGT+TGG	+	chr3.1:81908913-81908932	MS.gene32854:intron	30.0%
	CAACTGAATTAATACCAACA+AGG	-	chr3.1:81909780-81909799	None:intergenic	30.0%
	CATGCATGTTACAGTATATT+TGG	+	chr3.1:81908332-81908351	MS.gene32854:intron	30.0%
	CTTGTTGGTATTAATTCAGT+TGG	+	chr3.1:81909778-81909797	MS.gene32854:CDS	30.0%
	GCTTTCTTAAGTTATCATGT+TGG	+	chr3.1:81908077-81908096	MS.gene32854:intron	30.0%
	GTAGATATGAAGCACATTTA+TGG	+	chr3.1:81908148-81908167	MS.gene32854:CDS	30.0%
	TAATGATTCCAAATGGAAGA+TGG	+	chr3.1:81909657-81909676	MS.gene32854:CDS	30.0%
	TACTGAATGTTCCAAGATAA+AGG	-	chr3.1:81909034-81909053	None:intergenic	30.0%
	TATTATTGCAGAAAAAGCAG+TGG	+	chr3.1:81908820-81908839	MS.gene32854:intron	30.0%
	TCTTTAGATTCTACCTCTAA+TGG	-	chr3.1:81907573-81907592	None:intergenic	30.0%
	TGGATGATTCTACCAAAATA+GGG	+	chr3.1:81909803-81909822	MS.gene32854:CDS	30.0%
	TGGTATTAATTCAGTTGGTT+TGG	+	chr3.1:81909783-81909802	MS.gene32854:CDS	30.0%
	TTGGATGATTCTACCAAAAT+AGG	+	chr3.1:81909802-81909821	MS.gene32854:CDS	30.0%
!	AGGAAATCTATTGACACTTT+TGG	+	chr3.1:81907906-81907925	MS.gene32854:CDS	30.0%
!	TGAAAAATGTGTCCCTATTT+TGG	-	chr3.1:81909818-81909837	None:intergenic	30.0%
!	TGAAAGTTGTTACCATTTTC+AGG	-	chr3.1:81907028-81907047	None:intergenic	30.0%
!!	ATCATGTTGGTGATTATCTT+TGG	+	chr3.1:81908090-81908109	MS.gene32854:intron	30.0%
!!	GCAATTTTTGATTCCATTGA+AGG	-	chr3.1:81909925-81909944	None:intergenic	30.0%
!!!	ACTTTTTCTAAGGCTGTAAA+GGG	+	chr3.1:81909291-81909310	MS.gene32854:intron	30.0%
!!!	GTGTTATTTGTTCATGCTTT+TGG	+	chr3.1:81908287-81908306	MS.gene32854:intron	30.0%
	AAACTACCATACTCAACTTG+AGG	+	chr3.1:81908666-81908685	MS.gene32854:CDS	35.0%
	AAACTTGTCACCAATGATGA+TGG	-	chr3.1:81909844-81909863	None:intergenic	35.0%
	AACTCAAGGTGGTTATGATA+TGG	+	chr3.1:81908469-81908488	MS.gene32854:intron	35.0%
	AAGGAAGGACAAACAAGAAA+AGG	+	chr3.1:81908186-81908205	MS.gene32854:CDS	35.0%
	AATGAAGAATATGACAAGGC+AGG	+	chr3.1:81908690-81908709	MS.gene32854:CDS	35.0%
	ACAATGGGTAGTGATGAATA+TGG	+	chr3.1:81907366-81907385	MS.gene32854:CDS	35.0%
	ACTATGGAGGCTAAAAAAAG+AGG	+	chr3.1:81907849-81907868	MS.gene32854:CDS	35.0%
	AGAACTAACAGCAACATTAG+TGG	-	chr3.1:81909947-81909966	None:intergenic	35.0%
	AGAGTTACTACTGTCATTCA+TGG	-	chr3.1:81906822-81906841	None:intergenic	35.0%
	AGATGGATCACTTTGTATCA+TGG	+	chr3.1:81907081-81907100	MS.gene32854:CDS	35.0%
	AGGAGAAGAAGTAGAAATGT+GGG	-	chr3.1:81906891-81906910	None:intergenic	35.0%
	ATCAAGCAGCTCTTAAGTAT+TGG	+	chr3.1:81908516-81908535	MS.gene32854:CDS	35.0%
	ATCTTCATCTCCTAAACTAG+AGG	+	chr3.1:81907326-81907345	MS.gene32854:CDS	35.0%
	ATGTCTTTACAAGACCTTGT+TGG	+	chr3.1:81909763-81909782	MS.gene32854:CDS	35.0%
	ATTTGTGTAGCTACTTGTGA+AGG	-	chr3.1:81909976-81909995	None:intergenic	35.0%
	CAAACAAGAAAAGGAAGACA+AGG	+	chr3.1:81908195-81908214	MS.gene32854:CDS	35.0%
	CATAACCAATCACTTGTGTT+TGG	-	chr3.1:81909227-81909246	None:intergenic	35.0%
	CTTTACAGCCTTAGAAAAAG+TGG	-	chr3.1:81909292-81909311	None:intergenic	35.0%
	GACAAATTGATTGAAGATGC+TGG	-	chr3.1:81910003-81910022	None:intergenic	35.0%
	GGTGAAAAATAGAAAGAAGC+AGG	-	chr3.1:81906968-81906987	None:intergenic	35.0%
	TATTCTCCATTAGGCATAGA+TGG	+	chr3.1:81908121-81908140	MS.gene32854:intron	35.0%
	TCAAGCAGCTCTTAAGTATT+GGG	+	chr3.1:81908517-81908536	MS.gene32854:CDS	35.0%
	TGCAAGGAGAATCAAAGATA+AGG	+	chr3.1:81909540-81909559	MS.gene32854:CDS	35.0%
	TGTTATGGTGTTCCTGAAAA+TGG	+	chr3.1:81907013-81907032	MS.gene32854:CDS	35.0%
	TTCAACACACATAAACTTCC+CGG	+	chr3.1:81908544-81908563	MS.gene32854:CDS	35.0%
	TTTGATTCCATTGAAGGCTT+TGG	-	chr3.1:81909919-81909938	None:intergenic	35.0%
!	AAAAAGCAGTGGATTCTCAA+GGG	+	chr3.1:81908831-81908850	MS.gene32854:CDS	35.0%
!	AAACTAGAGGATTTTCTTGG+TGG	+	chr3.1:81907339-81907358	MS.gene32854:CDS	35.0%
!	AAGATTCTGAAAGAAGGTCA+AGG	-	chr3.1:81907488-81907507	None:intergenic	35.0%
!	AAGCACACAACACATTTTGA+AGG	-	chr3.1:81907173-81907192	None:intergenic	35.0%
!	AGATTCTGAAAGAAGGTCAA+GGG	-	chr3.1:81907487-81907506	None:intergenic	35.0%
!	AGCACACAACACATTTTGAA+GGG	-	chr3.1:81907172-81907191	None:intergenic	35.0%
!	CCAAGAAAATCCTCTAGTTT+AGG	-	chr3.1:81907339-81907358	None:intergenic	35.0%
!	CCTAAACTAGAGGATTTTCT+TGG	+	chr3.1:81907336-81907355	MS.gene32854:CDS	35.0%
!	CTAATGTTGCTGTTAGTTCT+TGG	+	chr3.1:81909947-81909966	MS.gene32854:CDS	35.0%
!	CTGAAGAAGTTTGAGTTTGT+TGG	-	chr3.1:81906931-81906950	None:intergenic	35.0%
!	GTCTGAAAGATTCTGAAAGA+AGG	-	chr3.1:81907494-81907513	None:intergenic	35.0%
!	TAACCAAGTGCTGCATAATA+TGG	-	chr3.1:81907531-81907550	None:intergenic	35.0%
!	TCTAGTTTAGGAGATGAAGA+TGG	-	chr3.1:81907327-81907346	None:intergenic	35.0%
!	TGAAATTTTAGGCACTCAAG+AGG	+	chr3.1:81909390-81909409	MS.gene32854:intron	35.0%
!	TGGTAATGATGATGATGATG+AGG	-	chr3.1:81906911-81906930	None:intergenic	35.0%
!	TTTTCCCAAACACAAGTGAT+TGG	+	chr3.1:81909219-81909238	MS.gene32854:intron	35.0%
!!	GTTGAGTTTGTTAAGTGAGA+AGG	-	chr3.1:81906989-81907008	None:intergenic	35.0%
!!	TCATTTCCTCAAGTTGAGTA+TGG	-	chr3.1:81908675-81908694	None:intergenic	35.0%
!!!	AATACCAGTTTTGAGGCATT+TGG	-	chr3.1:81907626-81907645	None:intergenic	35.0%
!!!	ATACCAGTTTTGAGGCATTT+GGG	-	chr3.1:81907625-81907644	None:intergenic	35.0%
!!!	CACTTTTTCTAAGGCTGTAA+AGG	+	chr3.1:81909290-81909309	MS.gene32854:intron	35.0%
	AACAGAATCAGTGACTATGG+AGG	+	chr3.1:81907836-81907855	MS.gene32854:CDS	40.0%
	ACTCTTTCCAAAGCCTTCAA+TGG	+	chr3.1:81909909-81909928	MS.gene32854:CDS	40.0%
	AGATGAACACTACTAGCATG+GGG	+	chr3.1:81907670-81907689	MS.gene32854:CDS	40.0%
	ATATGGTAGCCATGAAAGTG+AGG	+	chr3.1:81907383-81907402	MS.gene32854:CDS	40.0%
	ATGGGATAACAGTTGCAAGA+AGG	+	chr3.1:81908167-81908186	MS.gene32854:CDS	40.0%
	CACAAGTAGCTACACAAATG+AGG	+	chr3.1:81909977-81909996	MS.gene32854:CDS	40.0%
	CAGAGAACATCTCAGTATAG+AGG	+	chr3.1:81907930-81907949	MS.gene32854:CDS	40.0%
	CAGATGAACACTACTAGCAT+GGG	+	chr3.1:81907669-81907688	MS.gene32854:CDS	40.0%
	CATCCATATTATGCAGCACT+TGG	+	chr3.1:81907525-81907544	MS.gene32854:CDS	40.0%
	CATTCCATGCAGCAAAAACT+GGG	-	chr3.1:81910032-81910051	None:intergenic	40.0%
	CCTATTTCAAGCACCATTAG+AGG	+	chr3.1:81907557-81907576	MS.gene32854:CDS	40.0%
	CCTCTAATGGTGCTTGAAAT+AGG	-	chr3.1:81907560-81907579	None:intergenic	40.0%
	CTAAGGAGATCAAATGTACC+GGG	-	chr3.1:81908565-81908584	None:intergenic	40.0%
	CTGCAAAACCATCTTCCATT+TGG	-	chr3.1:81909668-81909687	None:intergenic	40.0%
	CTTGGTAGATCACAATCACA+AGG	+	chr3.1:81907109-81907128	MS.gene32854:CDS	40.0%
	CTTTGTATCATGGAAGCTCT+TGG	+	chr3.1:81907091-81907110	MS.gene32854:CDS	40.0%
	GAGGAGAAGAAGTAGAAATG+TGG	-	chr3.1:81906892-81906911	None:intergenic	40.0%
	GATAACAGTTGCAAGAAGGA+AGG	+	chr3.1:81908171-81908190	MS.gene32854:CDS	40.0%
	GCTAAGGAGATCAAATGTAC+CGG	-	chr3.1:81908566-81908585	None:intergenic	40.0%
	GCTAGCAATACTCTTCTTTC+TGG	+	chr3.1:81909511-81909530	MS.gene32854:CDS	40.0%
	GGAGAAGAAGTAGAAATGTG+GGG	-	chr3.1:81906890-81906909	None:intergenic	40.0%
	GGGAACTAGAAACATCAACA+TGG	-	chr3.1:81907605-81907624	None:intergenic	40.0%
	GGGAAGTTTATGTGTGTTGA+AGG	-	chr3.1:81908545-81908564	None:intergenic	40.0%
	GGTAACAAGGACCTTTATCT+TGG	+	chr3.1:81909020-81909039	MS.gene32854:CDS	40.0%
	GTAGATCACAATCACAAGGT+TGG	+	chr3.1:81907113-81907132	MS.gene32854:intron	40.0%
	GTAGCAGCAATAAAATTCCG+TGG	+	chr3.1:81909433-81909452	MS.gene32854:CDS	40.0%
	GTCAAAAGCAGCCTATTCAT+AGG	+	chr3.1:81907886-81907905	MS.gene32854:CDS	40.0%
	GTTATGCCTATCAAGTCAGA+TGG	+	chr3.1:81907064-81907083	MS.gene32854:CDS	40.0%
	TATGCAGCACTTGGTTATCA+TGG	+	chr3.1:81907534-81907553	MS.gene32854:CDS	40.0%
	TATTCATGAACTTCGGTCTG+TGG	-	chr3.1:81909568-81909587	None:intergenic	40.0%
	TCATTCCATGCAGCAAAAAC+TGG	-	chr3.1:81910033-81910052	None:intergenic	40.0%
	TCTTCTGTTGTTGGTACTGT+AGG	+	chr3.1:81907723-81907742	MS.gene32854:CDS	40.0%
	TGGAACAGAATCAGTGACTA+TGG	+	chr3.1:81907833-81907852	MS.gene32854:CDS	40.0%
	TTGTAACAGCATTTGCTCCA+CGG	-	chr3.1:81909453-81909472	None:intergenic	40.0%
!	AAAAGCAGTGGATTCTCAAG+GGG	+	chr3.1:81908832-81908851	MS.gene32854:CDS	40.0%
!	AGTGATCCATCTGACTTGAT+AGG	-	chr3.1:81907073-81907092	None:intergenic	40.0%
!	CAAGCAGCTCTTAAGTATTG+GGG	+	chr3.1:81908518-81908537	MS.gene32854:CDS	40.0%
!	GAAAAAGCAGTGGATTCTCA+AGG	+	chr3.1:81908830-81908849	MS.gene32854:intron	40.0%
!	GAAGCAGAATACCAGTTTTG+AGG	-	chr3.1:81907633-81907652	None:intergenic	40.0%
!	GATGAGGATCTGCATTTTGT+TGG	-	chr3.1:81907446-81907465	None:intergenic	40.0%
!	TGGTCTGTATTAGCTTGATG+AGG	-	chr3.1:81907462-81907481	None:intergenic	40.0%
!	TTTTGCAGACATCACCAACA+TGG	+	chr3.1:81908966-81908985	MS.gene32854:intron	40.0%
!!	TTTCTTGGTGGTGCAACAAT+GGG	+	chr3.1:81907351-81907370	MS.gene32854:CDS	40.0%
!!!	TTTTCTTGGTGGTGCAACAA+TGG	+	chr3.1:81907350-81907369	MS.gene32854:CDS	40.0%
	AAAAGAGGAGCTGCTAAGCT+TGG	+	chr3.1:81907864-81907883	MS.gene32854:CDS	45.0%
	AAGCCATTGCCTCACTTTCA+TGG	-	chr3.1:81907395-81907414	None:intergenic	45.0%
	AATGTATCGAGCCAGACCAA+TGG	+	chr3.1:81909601-81909620	MS.gene32854:CDS	45.0%
	ACACAGCTAGATTGAGAACC+AGG	-	chr3.1:81907786-81907805	None:intergenic	45.0%
	ACCAGTCCATCTATGCCTAA+TGG	-	chr3.1:81908130-81908149	None:intergenic	45.0%
	ACTAGCATGGGGAGTCATAA+TGG	+	chr3.1:81907681-81907700	MS.gene32854:CDS	45.0%
	CACAGCTAGATTGAGAACCA+GGG	-	chr3.1:81907785-81907804	None:intergenic	45.0%
	CTGAAAGAAGGTCAAGGGAA+TGG	-	chr3.1:81907482-81907501	None:intergenic	45.0%
	GAAAGAAGCAGGAACAGTGT+TGG	-	chr3.1:81906957-81906976	None:intergenic	45.0%
	GCAGATGAACACTACTAGCA+TGG	+	chr3.1:81907668-81907687	MS.gene32854:CDS	45.0%
	GGTGGTTCTTCTTCTGTTGT+TGG	+	chr3.1:81907714-81907733	MS.gene32854:CDS	45.0%
	GTTCCCAAATGCCTCAAAAC+TGG	+	chr3.1:81907619-81907638	MS.gene32854:CDS	45.0%
	GTTGTTGGTACTGTAGGAAG+TGG	+	chr3.1:81907729-81907748	MS.gene32854:CDS	45.0%
	TAGCCATGAAAGTGAGGCAA+TGG	+	chr3.1:81907389-81907408	MS.gene32854:CDS	45.0%
	TCCATTAGGCATAGATGGAC+TGG	+	chr3.1:81908126-81908145	MS.gene32854:intron	45.0%
	TGGAAGAGTTGCTGGTAACA+AGG	+	chr3.1:81909007-81909026	MS.gene32854:CDS	45.0%
	TTAAGCCTTTCAATGAGCCC+TGG	+	chr3.1:81907765-81907784	MS.gene32854:CDS	45.0%
!	ACTTCCCAGTTTTTGCTGCA+TGG	+	chr3.1:81910025-81910044	MS.gene32854:CDS	45.0%
!	TTCTTTCTGGTCAGCATGCA+AGG	+	chr3.1:81909524-81909543	MS.gene32854:CDS	45.0%
!!	AGGCTTTGGAAAGAGTAGTG+AGG	-	chr3.1:81909905-81909924	None:intergenic	45.0%
!!	AGTGAGGGAGCAGTTTTATC+AGG	-	chr3.1:81909889-81909908	None:intergenic	45.0%
!!	CTCAGTATAGAGGTGTCACA+AGG	+	chr3.1:81907940-81907959	MS.gene32854:CDS	45.0%
!!	GGCTTTGGAAAGAGTAGTGA+GGG	-	chr3.1:81909904-81909923	None:intergenic	45.0%
!!	GTCACTGATTCTGTTCCAGA+AGG	-	chr3.1:81907831-81907850	None:intergenic	45.0%
	AACATGGTAGGTGGCAAGCA+AGG	+	chr3.1:81908982-81909001	MS.gene32854:CDS	50.0%
	AGCATGGGGAGTCATAATGG+TGG	+	chr3.1:81907684-81907703	MS.gene32854:CDS	50.0%
	CCAGAAGGTGAGATCTGTCT+AGG	-	chr3.1:81907816-81907835	None:intergenic	50.0%
	CCTAGACAGATCTCACCTTC+TGG	+	chr3.1:81907813-81907832	MS.gene32854:CDS	50.0%
	GACATCACCAACATGGTAGG+TGG	+	chr3.1:81908973-81908992	MS.gene32854:intron	50.0%
	GAGAACCAGGGCTCATTGAA+AGG	-	chr3.1:81907773-81907792	None:intergenic	50.0%
	GCAAGGATTGGAAGAGTTGC+TGG	+	chr3.1:81908999-81909018	MS.gene32854:CDS	50.0%
	GCAGACATCACCAACATGGT+AGG	+	chr3.1:81908970-81908989	MS.gene32854:intron	50.0%
	TTGCTTGCCACCTACCATGT+TGG	-	chr3.1:81908983-81909002	None:intergenic	50.0%
!	AGGGGTGCATCAATGTACAG+AGG	+	chr3.1:81908850-81908869	MS.gene32854:CDS	50.0%
!	AGTCATAATGGTGGAGCTGG+TGG	+	chr3.1:81907693-81907712	MS.gene32854:CDS	50.0%
!	CAATGTACAGAGGCGTGACA+AGG	+	chr3.1:81908860-81908879	MS.gene32854:CDS	50.0%
	CAGCAGCTTCTCCATTGGTC+TGG	-	chr3.1:81909615-81909634	None:intergenic	55.0%
	CGCTTCAGCAGCTTCTCCAT+TGG	-	chr3.1:81909620-81909639	None:intergenic	55.0%
	GGTAGGTGGCAAGCAAGGAT+TGG	+	chr3.1:81908987-81909006	MS.gene32854:CDS	55.0%
!	CATAATGGTGGAGCTGGTGG+TGG	+	chr3.1:81907696-81907715	MS.gene32854:CDS	55.0%
!	GGGAGTCATAATGGTGGAGC+TGG	+	chr3.1:81907690-81907709	MS.gene32854:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	81906812	81910059	81906812	ID=MS.gene32854
chr3.1	mRNA	81906812	81910059	81906812	ID=MS.gene32854.t1;Parent=MS.gene32854
chr3.1	exon	81906812	81906872	81906812	ID=MS.gene32854.t1.exon1;Parent=MS.gene32854.t1
chr3.1	CDS	81906812	81906872	81906812	ID=cds.MS.gene32854.t1;Parent=MS.gene32854.t1
chr3.1	exon	81906951	81907130	81906951	ID=MS.gene32854.t1.exon2;Parent=MS.gene32854.t1
chr3.1	CDS	81906951	81907130	81906951	ID=cds.MS.gene32854.t1;Parent=MS.gene32854.t1
chr3.1	exon	81907316	81907961	81907316	ID=MS.gene32854.t1.exon3;Parent=MS.gene32854.t1
chr3.1	CDS	81907316	81907961	81907316	ID=cds.MS.gene32854.t1;Parent=MS.gene32854.t1
chr3.1	exon	81908134	81908216	81908134	ID=MS.gene32854.t1.exon4;Parent=MS.gene32854.t1
chr3.1	CDS	81908134	81908216	81908134	ID=cds.MS.gene32854.t1;Parent=MS.gene32854.t1
chr3.1	exon	81908346	81908354	81908346	ID=MS.gene32854.t1.exon5;Parent=MS.gene32854.t1
chr3.1	CDS	81908346	81908354	81908346	ID=cds.MS.gene32854.t1;Parent=MS.gene32854.t1
chr3.1	exon	81908477	81908565	81908477	ID=MS.gene32854.t1.exon6;Parent=MS.gene32854.t1
chr3.1	CDS	81908477	81908565	81908477	ID=cds.MS.gene32854.t1;Parent=MS.gene32854.t1
chr3.1	exon	81908661	81908734	81908661	ID=MS.gene32854.t1.exon7;Parent=MS.gene32854.t1
chr3.1	CDS	81908661	81908734	81908661	ID=cds.MS.gene32854.t1;Parent=MS.gene32854.t1
chr3.1	exon	81908831	81908881	81908831	ID=MS.gene32854.t1.exon8;Parent=MS.gene32854.t1
chr3.1	CDS	81908831	81908881	81908831	ID=cds.MS.gene32854.t1;Parent=MS.gene32854.t1
chr3.1	exon	81908974	81909050	81908974	ID=MS.gene32854.t1.exon9;Parent=MS.gene32854.t1
chr3.1	CDS	81908974	81909050	81908974	ID=cds.MS.gene32854.t1;Parent=MS.gene32854.t1
chr3.1	exon	81909401	81910059	81909401	ID=MS.gene32854.t1.exon10;Parent=MS.gene32854.t1
chr3.1	CDS	81909401	81910059	81909401	ID=cds.MS.gene32854.t1;Parent=MS.gene32854.t1

Gene Sequence

>MS.gene32854

ATGAAGTCCATGAATGACAGTAGTAACTCTGATGACAACAATCATAACAACAATTGGTTAGGTTTCTCTCTCTCACCCCACATTTCTACTTCTTCTCCTCATCATCATCATCATTACCAACAAACTCAAACTTCTTCAGTTTCCAACACTGTTCCTGCTTCTTTCTATTTTTCACCTTCTCACTTAACAAACTCAACAATCTGTTATGGTGTTCCTGAAAATGGTAACAACTTTCATTCACCTAATTTGACTGTTATGCCTATCAAGTCAGATGGATCACTTTGTATCATGGAAGCTCTTGGTAGATCACAATCACAAGGTTGGTTTCTGTCAAAAAATACTACTTTTTTTACTATTCCCTTCAAAATGTGTTGTGTGCTTTAAAAAGTTTTTATTTTTATGTCCACCAAAAAAAAGTTGTTATTTTTATATCTTTAATTAATGTTAGATATGGCCCTGTAATGATTATGTTGTTTTCTTGTTTTTATTATTATATGATTGAAGTGATGGTGCCATCTTCATCTCCTAAACTAGAGGATTTTCTTGGTGGTGCAACAATGGGTAGTGATGAATATGGTAGCCATGAAAGTGAGGCAATGGCTTTAAGCTTAGACAGCATCTACTATAATAACCAACAAAATGCAGATCCTCATCAAGCTAATACAGACCATTCCCTTGACCTTCTTTCAGAATCTTTCAGACAACAGACATCACATCCATATTATGCAGCACTTGGTTATCATGGCCTATTTCAAGCACCATTAGAGGTAGAATCTAAAGAAAACATAAACCATGTTGATGTTTCTAGTTCCCAAATGCCTCAAAACTGGTATTCTGCTTCTCAAGCTTTAGAGCAGCAGATGAACACTACTAGCATGGGGAGTCATAATGGTGGAGCTGGTGGTGGTTCTTCTTCTGTTGTTGGTACTGTAGGAAGTGGCGAGTTACAATCTTTAAGCCTTTCAATGAGCCCTGGTTCTCAATCTAGCTGTGTTACTGTTCCTAGACAGATCTCACCTTCTGGAACAGAATCAGTGACTATGGAGGCTAAAAAAAGAGGAGCTGCTAAGCTTGGTCAAAAGCAGCCTATTCATAGGAAATCTATTGACACTTTTGGACAGAGAACATCTCAGTATAGAGGTGTCACAAGGTAAGTTTGTGTTGATTATTATTTTATTTTTTTTATCTCATAGTGTGTTCTTCATTGTTTGATAAAAGTAGTCACATTTAACTATACTTTGTCTAGTATAATATGTTTGTGTTGTGCTTTCTTAAGTTATCATGTTGGTGATTATCTTTGGATTTTGTTTATTCTCCATTAGGCATAGATGGACTGGTAGATATGAAGCACATTTATGGGATAACAGTTGCAAGAAGGAAGGACAAACAAGAAAAGGAAGACAAGGTTAGTGACAAAAACAGTAGTCAAGTTTTTGAACTAGTACTTTTTTCCTACTAAAATTTTATACTAACACGTGTTATTTGTTCATGCTTTTGGATGATGATTTAATCAAACTTTGCATGCATGTTACAGTATATTTGGGTAAGTCTTGAGTTTCTCCTTCACCAAGTAAAAGAAAAAACAAACAATACTTTCAAGTTTCACCATCATTGCTATTACTGTTTCTAATTTTGACTTTGTTGGTTTTATTTTTTGAACTCAAGGTGGTTATGATATGGAAGAGAAAGCTGCAAGAGCTTATGATCAAGCAGCTCTTAAGTATTGGGGACCTTCAACACACATAAACTTCCCGGTACATTTGATCTCCTTAGCATTTTCTTGTTAGCTTTGAATCTTGTATTATTATGTGCTTAAGAATCACATTTTTTATTCTCTCTTATTTTGCAGCTAGAAAACTACCATACTCAACTTGAGGAAATGAAGAATATGACAAGGCAGGAATATGTTGCACATTTGAGAAGGTACATAAATCTGTTAATTGGCAATTATTTTGTAGTAAAAAGGTTAACATAATTATATGACAATCATGATGGATTAGTATTCATTTATTATTGCAGAAAAAGCAGTGGATTCTCAAGGGGTGCATCAATGTACAGAGGCGTGACAAGGTTTATTCAGTTCTCTTGTCCCTTTTTTTTCATTTGAATTTGTCACACTGTTGGTTTTTGAGCTAAATCACTTTTTTTTTTATGTTTTGCAGACATCACCAACATGGTAGGTGGCAAGCAAGGATTGGAAGAGTTGCTGGTAACAAGGACCTTTATCTTGGAACATTCAGTAAGTAGATTCCTCCAACATTGTTACTAAAAATGTGTTAACAAAAGATTTAAGCATTTAAATTATGAACAAGGGAATGTTTATGTATGTTTAAAGTTCAGCATCGTTTAAAGTAAATTCCTTCATAGGAATTTAATTTACTTATGTCATTTTCTGCTTCAGTATTTCTTTTCCCAAACACAAGTGATTGGTTATGCTTTTATAAGGCTGAACATGTTACTACTTTGAGCACAAAATTCCACTTTTTCTAAGGCTGTAAAGGGAATCTCTAATAGAATGGTGTAAGGAAAGTTTAGAATTTTGTAATGCCTTTTTGTAATTTTTTTTCTTTCTTGGAAATGAAATTTTAGGCACTCAAGAGGAAGCAGCTGAAGCATATGATGTAGCAGCAATAAAATTCCGTGGAGCAAATGCTGTTACAAATTTTGACATATCTAAATATGATGTTGAAAGGATCATGGCTAGCAATACTCTTCTTTCTGGTCAGCATGCAAGGAGAATCAAAGATAAGGATCCACAGACCGAAGTTCATGAATATAATCATAGTACTAATGTATCGAGCCAGACCAATGGAGAAGCTGCTGAAGCGCAAAACGTTAACGAAAATAATGATTCCAAATGGAAGATGGTTTTGCAGCAACAGCAATCAAATAGTTGTGATCAAAAAATCATTGTGAATTCTGATGGAAGTTACAAGAATTCTGATTTCTCTATGTCTTTACAAGACCTTGTTGGTATTAATTCAGTTGGTTTGGATGATTCTACCAAAATAGGGACACATTTTTCAAATCCATCATCATTGGTGACAAGTTTAAGCAGTTCAAGAGAAGCTAGTCCTGATAAAACTGCTCCCTCACTACTCTTTCCAAAGCCTTCAATGGAATCAAAAATTGCCACTAATGTTGCTGTTAGTTCTTGGTTTCCTTCACAAGTAGCTACACAAATGAGGCCAGCATCTTCAATCAATTTGTCTCACTTCCCAGTTTTTGCTGCATGGAATGAGACCTAG

Protein sequence

>MS.gene32854.t1

MKSMNDSSNSDDNNHNNNWLVSNTVPASFYFSPSHLTNSTICYGVPENGNNFHSPNLTVMPIKSDGSLCIMEALGRSQSQVMVPSSSPKLEDFLGGATMGSDEYGSHESEAMALSLDSIYYNNQQNADPHQANTDHSLDLLSESFRQQTSHPYYAALGYHGLFQAPLEVESKENINHVDVSSSQMPQNWYSASQALEQQMNTTSMGSHNGGAGGGSSSVVGTVGSGELQSLSLSMSPGSQSSCVTVPRQISPSGTESVTMEAKKRGAAKLGQKQPIHRKSIDTFGQRTSQYRGVTRHRWTGRYEAHLWDNSCKKEGQTRKGRQVYLGGYDMEEKAARAYDQAALKYWGPSTHINFPLENYHTQLEEMKNMTRQEYVAHLRRKSSGFSRGASMYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFSTQEEAAEAYDVAAIKFRGANAVTNFDISKYDVERIMASNTLLSGQHARRIKDKDPQTEVHEYNHSTNVSSQTNGEAAEAQNVNENNDSKWKMVLQQQQSNSCDQKIIVNSDGSYKNSDFSMSLQDLVGINSVGLDDSTKIGTHFSNPSSLVTSLSSSREASPDKTAPSLLFPKPSMESKIATNVAVSSWFPSQVATQMRPASSINLSHFPVFAAWNET