AlfalfaGEDB | MS.gene059425

M. sativa cultivar XinJiangDaYe / MS.gene059425

KEGG: -
GO: -
PFAM: PF05911.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059425.t1	XP_003607925.1	81.6	141	6	1	23	143	784	924	3.90E-47	197.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059425.t1	Q9SLN1	42.9	112	50	3	36	134	702	812	6.9e-08	58.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059425.t1	G7JK12	81.6	141	6	1	23	143	784	924	2.8e-47	197.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053536	MS.gene059425	0.876555	1.27E-68	-1.69E-46
MS.gene053538	MS.gene059425	0.892632	1.33E-74	-1.69E-46
MS.gene053540	MS.gene059425	0.856294	3.55E-62	-1.69E-46
MS.gene05894	MS.gene059425	0.811052	8.49E-51	-1.69E-46
MS.gene059424	MS.gene059425	0.880278	6.26E-70	-1.69E-46
MS.gene059425	MS.gene066010	0.807729	4.38E-50	-1.69E-46
MS.gene059425	MS.gene07246	0.801274	9.73E-49	-1.69E-46
MS.gene059425	MS.gene26380	0.811361	7.28E-51	-1.69E-46
MS.gene059425	MS.gene26464	0.876787	1.06E-68	-1.69E-46
MS.gene059425	MS.gene26465	0.854782	9.79E-62	-1.69E-46
MS.gene059425	MS.gene26659	0.803862	2.85E-49	-1.69E-46
MS.gene059425	MS.gene32024	0.807325	5.34E-50	-1.69E-46
MS.gene059425	MS.gene32854	0.816101	6.58E-52	-1.69E-46
MS.gene059425	MS.gene36880	0.804876	1.75E-49	-1.69E-46
MS.gene059425	MS.gene37460	0.8146	1.42E-51	-1.69E-46
MS.gene059425	MS.gene38829	0.80991	1.50E-50	-1.69E-46
MS.gene059425	MS.gene39297	0.829402	5.25E-55	-1.69E-46
MS.gene059425	MS.gene44437	0.800051	1.73E-48	-1.69E-46
MS.gene059425	MS.gene45937	0.801363	9.33E-49	-1.69E-46
MS.gene059425	MS.gene56169	0.810046	1.40E-50	-1.69E-46
MS.gene059425	MS.gene58836	0.816041	6.78E-52	-1.69E-46
MS.gene059425	MS.gene62491	0.81284	3.46E-51	-1.69E-46
MS.gene059425	MS.gene72487	0.813059	3.10E-51	-1.69E-46
MS.gene059425	MS.gene80266	0.802683	4.99E-49	-1.69E-46
MS.gene059425	MS.gene92988	0.801739	7.81E-49	-1.69E-46
MS.gene059425	MS.gene99595	0.80813	3.60E-50	-1.69E-46
MS.gene059425	MS.gene99949	0.815777	7.77E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059425.t1	MTR_4g085570	83.436	163	7	2	1	143	762	924	4.31e-80	254
MS.gene059425.t1	MTR_4g085570	68.539	89	25	1	1	86	762	850	6.40e-31	116
MS.gene059425.t1	MTR_5g016830	37.870	169	75	4	2	143	620	785	6.84e-25	99.4
MS.gene059425.t1	MTR_4g094848	37.931	174	74	3	1	142	714	885	1.54e-23	95.5
MS.gene059425.t1	MTR_4g094848	37.931	174	74	3	1	142	826	997	1.63e-23	95.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059425.t1	AT3G19370	43.537	147	54	4	2	136	514	643	7.77e-25	99.4
MS.gene059425.t1	AT3G19370	43.537	147	54	4	2	136	514	643	7.77e-25	99.4
MS.gene059425.t1	AT3G19370	43.537	147	54	4	2	136	514	643	7.77e-25	99.4
MS.gene059425.t1	AT2G23360	48.148	81	29	2	67	134	732	812	9.04e-11	58.9

Find 22 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATGTTATTTAACTTTGCC+TGG	0.349712	4.3:+27226040	None:intergenic
ATGTCTTGAGCTTCAACTTC+AGG	0.382553	4.3:-27225947	MS.gene059425:CDS
CATCATCCTCGGCTTGCATA+TGG	0.403033	4.3:+27225382	None:intergenic
TGTATCCACGACTAGTTCTA+TGG	0.421332	4.3:-27225454	MS.gene059425:CDS
TTCAATCTGCTCTTCAACCA+TGG	0.435146	4.3:+27226110	None:intergenic
ATCAAGTTCTTCTTCTGAGA+TGG	0.447467	4.3:+27225426	None:intergenic
CTCTTCTTTAACATCATCCT+CGG	0.449126	4.3:+27225371	None:intergenic
GTCATTAGAATTTGAGTTGG+AGG	0.455095	4.3:-27226004	MS.gene059425:CDS
CAAGGTTGGCAATACTCTCT+TGG	0.463481	4.3:+27225532	None:intergenic
TTTGAGTTGTTTCCCAAGGT+TGG	0.472439	4.3:+27225518	None:intergenic
CTTGAGCTTCAACTTCAGGT+TGG	0.478788	4.3:-27225943	MS.gene059425:CDS
GTGCTTTGAGTTGTTTCCCA+AGG	0.515309	4.3:+27225514	None:intergenic
TTCGTCATTAGAATTTGAGT+TGG	0.520970	4.3:-27226007	MS.gene059425:CDS
GTTCTTCTTCTGAGATGGAA+TGG	0.529032	4.3:+27225431	None:intergenic
CAAGAGAGTATTGCCAACCT+TGG	0.531537	4.3:-27225531	MS.gene059425:CDS
GATGTTATTTAACTTTGCCT+GGG	0.533839	4.3:+27226041	None:intergenic
AAGAGAGTATTGCCAACCTT+GGG	0.545467	4.3:-27225530	MS.gene059425:CDS
GCTTCAACTTCAGGTTGGAA+AGG	0.573605	4.3:-27225938	MS.gene059425:intron
TTGCATATGGTTGAGTAGTG+AGG	0.619906	4.3:+27225395	None:intergenic
CACACAACTTACAATAGCCC+AGG	0.622098	4.3:-27226058	MS.gene059425:CDS
AATGGCCATAGAACTAGTCG+TGG	0.659625	4.3:+27225449	None:intergenic
ACTCAACCATATGCAAGCCG+AGG	0.681575	4.3:-27225388	MS.gene059425:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATCTCAATGAAAAAAAAATC+AGG	-	chr4.3:27225872-27225891	MS.gene059425:intron	20.0%
!!	TCTCAATGAAAAAAAAATCA+GGG	-	chr4.3:27225873-27225892	MS.gene059425:intron	20.0%
!!!	TTTTTTTTTGTATGTTGCTA+TGG	-	chr4.3:27225782-27225801	MS.gene059425:intron	20.0%
!	AAGCAAAAGAAAATTGCAAA+AGG	+	chr4.3:27225606-27225625	None:intergenic	25.0%
!	TATCAAACTGTAAGAAGTTT+AGG	-	chr4.3:27225714-27225733	MS.gene059425:intron	25.0%
	AATGAAAAAAAAATCAGGGC+TGG	-	chr4.3:27225877-27225896	MS.gene059425:intron	30.0%
	ATGAAAAAAAAATCAGGGCT+GGG	-	chr4.3:27225878-27225897	MS.gene059425:intron	30.0%
	TTCGTCATTAGAATTTGAGT+TGG	-	chr4.3:27225460-27225479	MS.gene059425:CDS	30.0%
!	AGATGTTATTTAACTTTGCC+TGG	+	chr4.3:27225430-27225449	None:intergenic	30.0%
!	GATGTTATTTAACTTTGCCT+GGG	+	chr4.3:27225429-27225448	None:intergenic	30.0%
!!	GCAATTTTCTTTTGCTTTTC+TGG	-	chr4.3:27225609-27225628	MS.gene059425:intron	30.0%
	AAAAGGCTAGAAACCGAAAA+GGG	+	chr4.3:27225589-27225608	None:intergenic	35.0%
	CTCTTCTTTAACATCATCCT+CGG	+	chr4.3:27226099-27226118	None:intergenic	35.0%
!	ATCAAGTTCTTCTTCTGAGA+TGG	+	chr4.3:27226044-27226063	None:intergenic	35.0%
!!	GTCATTAGAATTTGAGTTGG+AGG	-	chr4.3:27225463-27225482	MS.gene059425:CDS	35.0%
!!	TGAAGCAGCACTTTTAGAAA+AGG	-	chr4.3:27225989-27226008	MS.gene059425:CDS	35.0%
	ATGTCTTGAGCTTCAACTTC+AGG	-	chr4.3:27225520-27225539	MS.gene059425:CDS	40.0%
	CAAAAGGCTAGAAACCGAAA+AGG	+	chr4.3:27225590-27225609	None:intergenic	40.0%
	CACTTCACATCTGCATTTGT+TGG	+	chr4.3:27225686-27225705	None:intergenic	40.0%
	TAGAAACCGAAAAGGGAGAA+GGG	+	chr4.3:27225582-27225601	None:intergenic	40.0%
	TTCCTTCTTTGCAATGCTGT+TGG	+	chr4.3:27225660-27225679	None:intergenic	40.0%
	TTGCATATGGTTGAGTAGTG+AGG	+	chr4.3:27226075-27226094	None:intergenic	40.0%
!	TTTTCACCCTTCTCCCTTTT+CGG	-	chr4.3:27225573-27225592	MS.gene059425:CDS	40.0%
!!	AAGAGAGTATTGCCAACCTT+GGG	-	chr4.3:27225937-27225956	MS.gene059425:intron	40.0%
!!	GTTCTTCTTCTGAGATGGAA+TGG	+	chr4.3:27226039-27226058	None:intergenic	40.0%
!!	TGTATCCACGACTAGTTCTA+TGG	-	chr4.3:27226013-27226032	MS.gene059425:CDS	40.0%
!!	TTCAGGTTGGAAAGGTAAAC+AGG	-	chr4.3:27225537-27225556	MS.gene059425:CDS	40.0%
!!	TTTGAGTTGTTTCCCAAGGT+TGG	+	chr4.3:27225952-27225971	None:intergenic	40.0%
	AATGGCCATAGAACTAGTCG+TGG	+	chr4.3:27226021-27226040	None:intergenic	45.0%
	CAAGGTTGGCAATACTCTCT+TGG	+	chr4.3:27225938-27225957	None:intergenic	45.0%
	CACACAACTTACAATAGCCC+AGG	-	chr4.3:27225409-27225428	MS.gene059425:CDS	45.0%
	CTAGAAACCGAAAAGGGAGA+AGG	+	chr4.3:27225583-27225602	None:intergenic	45.0%
	CTTGAGCTTCAACTTCAGGT+TGG	-	chr4.3:27225524-27225543	MS.gene059425:CDS	45.0%
	GGACAAAAAGAACTCGTGTG+AGG	-	chr4.3:27225484-27225503	MS.gene059425:CDS	45.0%
	TGCCAACAGCATTGCAAAGA+AGG	-	chr4.3:27225655-27225674	MS.gene059425:intron	45.0%
!!	CAAGAGAGTATTGCCAACCT+TGG	-	chr4.3:27225936-27225955	MS.gene059425:intron	45.0%
!!	GCTTCAACTTCAGGTTGGAA+AGG	-	chr4.3:27225529-27225548	MS.gene059425:CDS	45.0%
!!	GTGCTTTGAGTTGTTTCCCA+AGG	+	chr4.3:27225956-27225975	None:intergenic	45.0%
	ACTCAACCATATGCAAGCCG+AGG	-	chr4.3:27226079-27226098	MS.gene059425:CDS	50.0%
!	CATCATCCTCGGCTTGCATA+TGG	+	chr4.3:27226088-27226107	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	27225359	27226130	27225359	ID=MS.gene059425
chr4.3	mRNA	27225359	27226130	27225359	ID=MS.gene059425.t1;Parent=MS.gene059425
chr4.3	exon	27225939	27226130	27225939	ID=MS.gene059425.t1.exon1;Parent=MS.gene059425.t1
chr4.3	CDS	27225939	27226130	27225939	ID=cds.MS.gene059425.t1;Parent=MS.gene059425.t1
chr4.3	exon	27225359	27225595	27225359	ID=MS.gene059425.t1.exon2;Parent=MS.gene059425.t1
chr4.3	CDS	27225359	27225595	27225359	ID=cds.MS.gene059425.t1;Parent=MS.gene059425.t1

Gene Sequence

>MS.gene059425

ATGGTTGAAGAGCAGATTGAAAAACAAATGATGATAAATGAAGATCTTGACACACAACTTACAATAGCCCAGGCAAAGTTAAATAACATCTTTCAAAAGTTTTCGTCATTAGAATTTGAGTTGGAGGACAAAAAGAACTCGTGTGAGGAATTAGAAGCAACATGTCTTGAGCTTCAACTTCAGGTTGGAAAGGTAAACAGGAAAAGATAATCTCTTTTCACCCTTCTCCCTTTTCGGTTTCTAGCCTTTTGCAATTTTCTTTTGCTTTTCTGGTTAATGAATCTGATTTTGTAAAATGCCAACAGCATTGCAAAGAAGGAATCTCCAACAAATGCAGATGTGAAGTGAAAAGATATATCAAACTGTAAGAAGTTTAGGTCTTATTTAACTCAAGATATATGTCGCAAACTATTATCAATTATATTTTTTTTTGTATGTTGCTATGGCATATCAATTGTTTGCATTATCAATTATATTTCTATTTTCATTTTGCATCAAACACTTAATCAACAAATCTCAATGAAAAAAAAATCAGGGCTGGGAGATCACAACAGCTTCATCAAAGTTAGCAGAGTGCCAAGAGAGTATTGCCAACCTTGGGAAACAACTCAAAGCACTTGCTTCATCTAATGAAGCAGCACTTTTAGAAAAGGTTGTATCCACGACTAGTTCTATGGCCATTCCATCTCAGAAGAAGAACTTGATCAAACGATCCTCACTACTCAACCATATGCAAGCCGAGGATGATGTTAAAGAAGAGATGCATAAGTCT

Protein sequence

>MS.gene059425.t1

MVEEQIEKQMMINEDLDTQLTIAQAKLNNIFQKFSSLEFELEDKKNSCEELEATCLELQLQVGKGWEITTASSKLAECQESIANLGKQLKALASSNEAALLEKVVSTTSSMAIPSQKKNLIKRSSLLNHMQAEDDVKEEMHKS