Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34294.t1 | XP_013454503.1 | 95.8 | 95 | 4 | 0 | 1 | 95 | 118 | 212 | 1.40E-43 | 185.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34294.t1 | Q9SUE0 | 86.2 | 87 | 12 | 0 | 7 | 93 | 127 | 213 | 3.0e-39 | 162.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34294.t1 | A0A072UFK1 | 95.8 | 95 | 4 | 0 | 1 | 95 | 118 | 212 | 9.8e-44 | 185.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050767 | MS.gene34294 | 0.818443 | 1.96E-52 | -1.69E-46 |
| MS.gene050770 | MS.gene34294 | 0.8208 | 5.66E-53 | -1.69E-46 |
| MS.gene050812 | MS.gene34294 | 0.823332 | 1.47E-53 | -1.69E-46 |
| MS.gene05101 | MS.gene34294 | 0.810315 | 1.23E-50 | -1.69E-46 |
| MS.gene051471 | MS.gene34294 | 0.811644 | 6.32E-51 | -1.69E-46 |
| MS.gene051666 | MS.gene34294 | 0.815204 | 1.04E-51 | -1.69E-46 |
| MS.gene052078 | MS.gene34294 | 0.820554 | 6.45E-53 | -1.69E-46 |
| MS.gene052188 | MS.gene34294 | 0.824172 | 9.32E-54 | -1.69E-46 |
| MS.gene052290 | MS.gene34294 | 0.807239 | 5.57E-50 | -1.69E-46 |
| MS.gene052310 | MS.gene34294 | 0.821466 | 3.98E-53 | -1.69E-46 |
| MS.gene052322 | MS.gene34294 | 0.820835 | 5.56E-53 | -1.69E-46 |
| MS.gene053009 | MS.gene34294 | 0.813597 | 2.36E-51 | -1.69E-46 |
| MS.gene053284 | MS.gene34294 | 0.88069 | 4.46E-70 | -1.69E-46 |
| MS.gene053285 | MS.gene34294 | 0.903906 | 2.14E-79 | -1.69E-46 |
| MS.gene054123 | MS.gene34294 | 0.807287 | 5.44E-50 | -1.69E-46 |
| MS.gene054376 | MS.gene34294 | 0.8064 | 8.38E-50 | -1.69E-46 |
| MS.gene055412 | MS.gene34294 | 0.80522 | 1.48E-49 | -1.69E-46 |
| MS.gene056719 | MS.gene34294 | 0.80895 | 2.41E-50 | -1.69E-46 |
| MS.gene056796 | MS.gene34294 | 0.82587 | 3.70E-54 | -1.69E-46 |
| MS.gene056897 | MS.gene34294 | 0.837298 | 5.64E-57 | -1.69E-46 |
| MS.gene057129 | MS.gene34294 | 0.835675 | 1.46E-56 | -1.69E-46 |
| MS.gene057295 | MS.gene34294 | 0.83105 | 2.08E-55 | -1.69E-46 |
| MS.gene058082 | MS.gene34294 | 0.841161 | 5.62E-58 | -1.69E-46 |
| MS.gene058276 | MS.gene34294 | 0.81354 | 2.43E-51 | -1.69E-46 |
| MS.gene058293 | MS.gene34294 | 0.844704 | 6.41E-59 | -1.69E-46 |
| MS.gene058365 | MS.gene34294 | 0.810172 | 1.32E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene34294 | 0.800573 | 1.35E-48 | -1.69E-46 |
| MS.gene058748 | MS.gene34294 | 0.829211 | 5.84E-55 | -1.69E-46 |
| MS.gene058787 | MS.gene34294 | 0.834179 | 3.48E-56 | -1.69E-46 |
| MS.gene05959 | MS.gene34294 | 0.819008 | 1.45E-52 | -1.69E-46 |
| MS.gene059676 | MS.gene34294 | 0.828675 | 7.88E-55 | -1.69E-46 |
| MS.gene059692 | MS.gene34294 | 0.809785 | 1.59E-50 | -1.69E-46 |
| MS.gene059695 | MS.gene34294 | 0.811763 | 5.95E-51 | -1.69E-46 |
| MS.gene059960 | MS.gene34294 | 0.828823 | 7.25E-55 | -1.69E-46 |
| MS.gene06090 | MS.gene34294 | 0.810982 | 8.79E-51 | -1.69E-46 |
| MS.gene061150 | MS.gene34294 | 0.807933 | 3.97E-50 | -1.69E-46 |
| MS.gene061200 | MS.gene34294 | 0.809025 | 2.32E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34294.t1 | MTR_4g005520 | 95.789 | 95 | 4 | 0 | 1 | 95 | 118 | 212 | 4.30e-62 | 187 |
| MS.gene34294.t1 | MTR_3g105100 | 47.368 | 76 | 35 | 3 | 15 | 89 | 120 | 191 | 2.63e-14 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34294.t1 | AT4G27990 | 85.227 | 88 | 13 | 0 | 7 | 94 | 127 | 214 | 8.55e-52 | 162 |
| MS.gene34294.t1 | AT3G07430 | 83.908 | 87 | 14 | 0 | 7 | 93 | 141 | 227 | 5.25e-51 | 160 |
| MS.gene34294.t1 | AT5G21920 | 50.000 | 76 | 33 | 3 | 15 | 89 | 133 | 204 | 8.24e-15 | 67.4 |
Find 24 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACAACCATTGTCTGCAATT+AGG | 0.283838 | 4.3:+88825318 | MS.gene34294:CDS |
| ACAACCATTGTCTGCAATTA+GGG | 0.307081 | 4.3:+88825319 | MS.gene34294:CDS |
| ACAGTGGTGTGTTGTTGGTT+AGG | 0.327645 | 4.3:+88825258 | MS.gene34294:CDS |
| ATCCTTATCTAAGTTTGTTT+AGG | 0.329465 | 4.3:+88825351 | MS.gene34294:CDS |
| CAGTGGTGTGTTGTTGGTTA+GGG | 0.363509 | 4.3:+88825259 | MS.gene34294:CDS |
| TAGCGGCGGGGTTGAAGAAA+TGG | 0.403666 | 4.3:+88825225 | MS.gene34294:CDS |
| TATTTACAGTGGTGTGTTGT+TGG | 0.416695 | 4.3:+88825253 | MS.gene34294:CDS |
| GACGGTGAACACGCCGTTGA+CGG | 0.439588 | 4.3:+88825199 | MS.gene34294:CDS |
| CCGTTGACGGTTGTAGCGGC+GGG | 0.441420 | 4.3:+88825212 | MS.gene34294:CDS |
| TGTCTTTCCCATGGAATGTT+GGG | 0.454889 | 4.3:-88825299 | None:intergenic |
| GTTGGTTTCCCAACATTCCA+TGG | 0.512936 | 4.3:+88825291 | MS.gene34294:CDS |
| GACAATGGTTGTCTTTCCCA+TGG | 0.515980 | 4.3:-88825308 | None:intergenic |
| TTCCTAAACAAACTTAGATA+AGG | 0.528049 | 4.3:-88825353 | None:intergenic |
| GCCGTTGACGGTTGTAGCGG+CGG | 0.535668 | 4.3:+88825211 | MS.gene34294:CDS |
| TTGGTTTCCCAACATTCCAT+GGG | 0.548737 | 4.3:+88825292 | MS.gene34294:CDS |
| CCCGCCGCTACAACCGTCAA+CGG | 0.573983 | 4.3:-88825212 | None:intergenic |
| TTGTCTTTCCCATGGAATGT+TGG | 0.574451 | 4.3:-88825300 | None:intergenic |
| AAAACAGCAAAAGCAAGAAG+AGG | 0.574512 | 4.3:-88825413 | None:intergenic |
| CACGCCGTTGACGGTTGTAG+CGG | 0.575214 | 4.3:+88825208 | MS.gene34294:CDS |
| CGTTGACGGTTGTAGCGGCG+GGG | 0.610796 | 4.3:+88825213 | MS.gene34294:CDS |
| GGCGGGGTTGAAGAAATGGT+TGG | 0.623671 | 4.3:+88825229 | MS.gene34294:CDS |
| AAATGGTTGGATATTTACAG+TGG | 0.642279 | 4.3:+88825242 | MS.gene34294:CDS |
| AGATCCCTAATTGCAGACAA+TGG | 0.662621 | 4.3:-88825323 | None:intergenic |
| GGATCTAAACAGGAACAGTG+AGG | 0.722011 | 4.3:-88825454 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCCTAAACAAACTTAGATA+AGG | - | chr4.3:88825356-88825375 | None:intergenic | 25.0% |
| !! | ATCCTTATCTAAGTTTGTTT+AGG | + | chr4.3:88825351-88825370 | MS.gene34294:CDS | 25.0% |
| !!! | ATATCAAGAGTATCAAAAAC+AGG | - | chr4.3:88825389-88825408 | None:intergenic | 25.0% |
| AAATGGTTGGATATTTACAG+TGG | + | chr4.3:88825242-88825261 | MS.gene34294:CDS | 30.0% | |
| AAAACAGCAAAAGCAAGAAG+AGG | - | chr4.3:88825416-88825435 | None:intergenic | 35.0% | |
| ACAACCATTGTCTGCAATTA+GGG | + | chr4.3:88825319-88825338 | MS.gene34294:CDS | 35.0% | |
| TATTTACAGTGGTGTGTTGT+TGG | + | chr4.3:88825253-88825272 | MS.gene34294:CDS | 35.0% | |
| !! | TCAAGAGTATCAAAAACAGG+TGG | - | chr4.3:88825386-88825405 | None:intergenic | 35.0% |
| !!! | CTTCTTGCTTTTGCTGTTTT+GGG | + | chr4.3:88825416-88825435 | MS.gene34294:CDS | 35.0% |
| !!! | TCTTCTTGCTTTTGCTGTTT+TGG | + | chr4.3:88825415-88825434 | MS.gene34294:CDS | 35.0% |
| AGATCCCTAATTGCAGACAA+TGG | - | chr4.3:88825326-88825345 | None:intergenic | 40.0% | |
| GACAACCATTGTCTGCAATT+AGG | + | chr4.3:88825318-88825337 | MS.gene34294:CDS | 40.0% | |
| TGTCTTTCCCATGGAATGTT+GGG | - | chr4.3:88825302-88825321 | None:intergenic | 40.0% | |
| TTGGTTTCCCAACATTCCAT+GGG | + | chr4.3:88825292-88825311 | MS.gene34294:CDS | 40.0% | |
| TTGTCTTTCCCATGGAATGT+TGG | - | chr4.3:88825303-88825322 | None:intergenic | 40.0% | |
| !!! | TGGTTAGGGTTTTGTTGAGT+TGG | + | chr4.3:88825273-88825292 | MS.gene34294:CDS | 40.0% |
| GACAATGGTTGTCTTTCCCA+TGG | - | chr4.3:88825311-88825330 | None:intergenic | 45.0% | |
| ! | ACAGTGGTGTGTTGTTGGTT+AGG | + | chr4.3:88825258-88825277 | MS.gene34294:CDS | 45.0% |
| ! | CAGTGGTGTGTTGTTGGTTA+GGG | + | chr4.3:88825259-88825278 | MS.gene34294:CDS | 45.0% |
| ! | GTTGGTTTCCCAACATTCCA+TGG | + | chr4.3:88825291-88825310 | MS.gene34294:CDS | 45.0% |
| !! | CCCGTCCTAGTCTTTTTCAT+AGG | - | chr4.3:88825179-88825198 | None:intergenic | 45.0% |
| GAAAAAGACTAGGACGGGGA+CGG | + | chr4.3:88825181-88825200 | MS.gene34294:CDS | 50.0% | |
| !! | GCTGTTTTGGGTACTCTTGC+TGG | + | chr4.3:88825428-88825447 | MS.gene34294:CDS | 50.0% |
| GGCGGGGTTGAAGAAATGGT+TGG | + | chr4.3:88825229-88825248 | MS.gene34294:CDS | 55.0% | |
| TAGCGGCGGGGTTGAAGAAA+TGG | + | chr4.3:88825225-88825244 | MS.gene34294:CDS | 55.0% | |
| CACGCCGTTGACGGTTGTAG+CGG | + | chr4.3:88825208-88825227 | MS.gene34294:CDS | 60.0% | |
| GACGGTGAACACGCCGTTGA+CGG | + | chr4.3:88825199-88825218 | MS.gene34294:CDS | 60.0% | |
| CCCGCCGCTACAACCGTCAA+CGG | - | chr4.3:88825215-88825234 | None:intergenic | 65.0% | |
| CCGTTGACGGTTGTAGCGGC+GGG | + | chr4.3:88825212-88825231 | MS.gene34294:CDS | 65.0% | |
| CGTTGACGGTTGTAGCGGCG+GGG | + | chr4.3:88825213-88825232 | MS.gene34294:CDS | 65.0% | |
| GCCGTTGACGGTTGTAGCGG+CGG | + | chr4.3:88825211-88825230 | MS.gene34294:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 88825179 | 88825466 | 88825179 | ID=MS.gene34294 |
| chr4.3 | mRNA | 88825179 | 88825466 | 88825179 | ID=MS.gene34294.t1;Parent=MS.gene34294 |
| chr4.3 | exon | 88825179 | 88825466 | 88825179 | ID=MS.gene34294.t1.exon1;Parent=MS.gene34294.t1 |
| chr4.3 | CDS | 88825179 | 88825466 | 88825179 | ID=cds.MS.gene34294.t1;Parent=MS.gene34294.t1 |
| Gene Sequence |
| Protein sequence |
