Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37301.t1 | RHN71126.1 | 96 | 654 | 25 | 1 | 1 | 654 | 1 | 653 | 2.20E-286 | 994.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37301.t1 | P54103 | 37.4 | 625 | 288 | 17 | 62 | 653 | 55 | 609 | 9.7e-57 | 223.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37301.t1 | A0A396IZE3 | 96.0 | 654 | 25 | 1 | 1 | 654 | 1 | 653 | 1.6e-286 | 994.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene37301.t1 | TF | MYB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene37301 | 0.815927 | 7.19E-52 | -1.69E-46 |
| MS.gene04918 | MS.gene37301 | 0.806712 | 7.20E-50 | -1.69E-46 |
| MS.gene04920 | MS.gene37301 | 0.810325 | 1.22E-50 | -1.69E-46 |
| MS.gene049983 | MS.gene37301 | 0.806271 | 8.92E-50 | -1.69E-46 |
| MS.gene050084 | MS.gene37301 | 0.807979 | 3.88E-50 | -1.69E-46 |
| MS.gene050085 | MS.gene37301 | 0.832283 | 1.03E-55 | -1.69E-46 |
| MS.gene050654 | MS.gene37301 | 0.83226 | 1.05E-55 | -1.69E-46 |
| MS.gene051003 | MS.gene37301 | 0.838914 | 2.17E-57 | -1.69E-46 |
| MS.gene051234 | MS.gene37301 | 0.807345 | 5.29E-50 | -1.69E-46 |
| MS.gene051363 | MS.gene37301 | 0.829714 | 4.41E-55 | -1.69E-46 |
| MS.gene051365 | MS.gene37301 | 0.824129 | 9.54E-54 | -1.69E-46 |
| MS.gene05188 | MS.gene37301 | 0.858766 | 6.58E-63 | -1.69E-46 |
| MS.gene051975 | MS.gene37301 | 0.831752 | 1.40E-55 | -1.69E-46 |
| MS.gene052072 | MS.gene37301 | 0.808827 | 2.56E-50 | -1.69E-46 |
| MS.gene052342 | MS.gene37301 | 0.824076 | 9.82E-54 | -1.69E-46 |
| MS.gene052434 | MS.gene37301 | 0.840647 | 7.67E-58 | -1.69E-46 |
| MS.gene052518 | MS.gene37301 | 0.829132 | 6.11E-55 | -1.69E-46 |
| MS.gene053058 | MS.gene37301 | 0.83003 | 3.69E-55 | -1.69E-46 |
| MS.gene053381 | MS.gene37301 | 0.810624 | 1.05E-50 | -1.69E-46 |
| MS.gene05517 | MS.gene37301 | 0.806427 | 8.27E-50 | -1.69E-46 |
| MS.gene055513 | MS.gene37301 | 0.810836 | 9.46E-51 | -1.69E-46 |
| MS.gene056181 | MS.gene37301 | 0.816963 | 4.22E-52 | -1.69E-46 |
| MS.gene056403 | MS.gene37301 | 0.834656 | 2.64E-56 | -1.69E-46 |
| MS.gene056605 | MS.gene37301 | 0.837189 | 6.02E-57 | -1.69E-46 |
| MS.gene056786 | MS.gene37301 | 0.810871 | 9.29E-51 | -1.69E-46 |
| MS.gene05689 | MS.gene37301 | 0.807907 | 4.02E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene37301 | 0.831871 | 1.31E-55 | -1.69E-46 |
| MS.gene057102 | MS.gene37301 | 0.802676 | 5.01E-49 | -1.69E-46 |
| MS.gene05781 | MS.gene37301 | 0.813884 | 2.04E-51 | -1.69E-46 |
| MS.gene058292 | MS.gene37301 | 0.808221 | 3.44E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene37301 | 0.87103 | 9.32E-67 | -1.69E-46 |
| MS.gene059901 | MS.gene37301 | -0.82509 | 5.66E-54 | -1.69E-46 |
| MS.gene06010 | MS.gene37301 | 0.83445 | 2.98E-56 | -1.69E-46 |
| MS.gene06011 | MS.gene37301 | 0.878067 | 3.79E-69 | -1.69E-46 |
| MS.gene060763 | MS.gene37301 | 0.877092 | 8.29E-69 | -1.69E-46 |
| MS.gene060830 | MS.gene37301 | 0.817427 | 3.32E-52 | -1.69E-46 |
| MS.gene060933 | MS.gene37301 | 0.805921 | 1.06E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene37301 | 0.827961 | 1.17E-54 | -1.69E-46 |
| MS.gene060937 | MS.gene37301 | 0.837457 | 5.14E-57 | -1.69E-46 |
| MS.gene061100 | MS.gene37301 | 0.808495 | 3.01E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37301.t1 | MTR_3g113680 | 97.554 | 654 | 15 | 1 | 1 | 654 | 1 | 653 | 0.0 | 1308 |
| MS.gene37301.t1 | MTR_7g109430 | 97.095 | 654 | 18 | 1 | 1 | 654 | 1 | 653 | 0.0 | 1306 |
| MS.gene37301.t1 | MTR_3g114060 | 81.720 | 279 | 43 | 2 | 378 | 654 | 62 | 334 | 1.30e-148 | 434 |
| MS.gene37301.t1 | MTR_8g093080 | 29.439 | 214 | 95 | 3 | 443 | 653 | 140 | 300 | 1.05e-20 | 93.6 |
| MS.gene37301.t1 | MTR_4g109510 | 30.417 | 240 | 115 | 9 | 98 | 300 | 9 | 233 | 3.01e-17 | 86.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37301.t1 | AT3G11450 | 63.939 | 660 | 210 | 11 | 4 | 654 | 7 | 647 | 0.0 | 734 |
| MS.gene37301.t1 | AT5G06110 | 59.492 | 669 | 239 | 10 | 2 | 651 | 5 | 660 | 0.0 | 713 |
| MS.gene37301.t1 | AT5G06110 | 59.492 | 669 | 239 | 10 | 2 | 651 | 5 | 660 | 0.0 | 713 |
| MS.gene37301.t1 | AT5G45420 | 31.959 | 194 | 87 | 3 | 459 | 652 | 158 | 306 | 7.78e-19 | 88.2 |
| MS.gene37301.t1 | AT5G45420 | 31.959 | 194 | 87 | 3 | 459 | 652 | 158 | 306 | 7.78e-19 | 88.2 |
| MS.gene37301.t1 | AT5G45420 | 31.959 | 194 | 87 | 3 | 459 | 652 | 158 | 306 | 7.78e-19 | 88.2 |
| MS.gene37301.t1 | AT1G74250 | 29.167 | 240 | 117 | 6 | 98 | 297 | 12 | 238 | 4.83e-16 | 82.4 |
Find 130 sgRNAs with CRISPR-Local
Find 155 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACTTCTTTGCCATATATT+TGG | 0.137476 | 3.1:+90517005 | None:intergenic |
| TTTCTCCTCTTCAGCAATTC+TGG | 0.180978 | 3.1:+90516966 | None:intergenic |
| GCAAGATCATTATGCATTAT+TGG | 0.181634 | 3.1:-90517658 | MS.gene37301:CDS |
| TCCTTTCTTCTCAGTATTCT+AGG | 0.194363 | 3.1:+90517063 | None:intergenic |
| CCATGATGAAGGAAAATTTC+AGG | 0.207011 | 3.1:-90516021 | MS.gene37301:CDS |
| CCATCTCCTTGAAGTTCCTT+TGG | 0.238251 | 3.1:+90516513 | None:intergenic |
| CTTTGGCTGTATCGGGCTTC+TGG | 0.244533 | 3.1:+90516416 | None:intergenic |
| CATCTCTGGTTAGCTTCCTT+TGG | 0.256785 | 3.1:+90516103 | None:intergenic |
| CAAGATCATTATGCATTATT+GGG | 0.286954 | 3.1:-90517657 | MS.gene37301:CDS |
| GTCTAATTGTCTTCCGGTTA+AGG | 0.294533 | 3.1:-90517868 | MS.gene37301:CDS |
| AGCTGAGAAGAAAAGGAAAA+AGG | 0.327832 | 3.1:-90517031 | MS.gene37301:CDS |
| ATTGTCAACTAGAGAAGAAT+TGG | 0.337451 | 3.1:-90516338 | MS.gene37301:CDS |
| TCATATTTCAACGCCTTAAC+CGG | 0.338185 | 3.1:+90517855 | None:intergenic |
| GAGAAAAGTAATCAACAAAA+TGG | 0.344685 | 3.1:-90516649 | MS.gene37301:CDS |
| CATCTCCTTGAAGTTCCTTT+GGG | 0.350239 | 3.1:+90516514 | None:intergenic |
| CTGAATCCCGAGATCATAAA+AGG | 0.353541 | 3.1:-90517182 | MS.gene37301:CDS |
| ATCTCTGGTTAGCTTCCTTT+GGG | 0.356444 | 3.1:+90516104 | None:intergenic |
| CGAGTAGCTGCAGCTGTACC+TGG | 0.359607 | 3.1:-90516076 | MS.gene37301:CDS |
| TACTCACAAGAGCTTGTAGA+TGG | 0.365954 | 3.1:-90517909 | MS.gene37301:CDS |
| AATGGCTATGTCAAGGCTAA+TGG | 0.383665 | 3.1:-90516631 | MS.gene37301:CDS |
| TGTTTCGTCTAATTGTCTTC+CGG | 0.396355 | 3.1:-90517874 | MS.gene37301:CDS |
| CTTCTGTTGCAAAGCAGCTT+CGG | 0.407096 | 3.1:+90516912 | None:intergenic |
| CAGCTACTCGTTCCCATCTC+TGG | 0.408539 | 3.1:+90516089 | None:intergenic |
| AGGCAAAAGCTGAGAAGAAA+AGG | 0.413153 | 3.1:-90517038 | MS.gene37301:CDS |
| TTGTTGATAATGCTTATAAG+AGG | 0.415278 | 3.1:-90517089 | MS.gene37301:CDS |
| AGCAAGAGCAAGAAGAAATC+TGG | 0.416590 | 3.1:-90517699 | MS.gene37301:CDS |
| ATTCTTCTCTAGTTGACAAT+GGG | 0.419152 | 3.1:+90516341 | None:intergenic |
| TCATCACCTAGAGATGGAAT+TGG | 0.419175 | 3.1:+90517306 | None:intergenic |
| CCTTTCTTCTCAGTATTCTA+GGG | 0.419637 | 3.1:+90517064 | None:intergenic |
| GAAGAATGTGGTTGAAGATA+AGG | 0.419917 | 3.1:-90517754 | MS.gene37301:CDS |
| CTGCTTGTTGCTGTCGGTAC+CGG | 0.420172 | 3.1:+90516217 | None:intergenic |
| TCAAAAGCTTTGGCTGTATC+GGG | 0.425452 | 3.1:+90516409 | None:intergenic |
| CAAGACTTCTACAAGGTGTT+TGG | 0.427058 | 3.1:-90517369 | MS.gene37301:CDS |
| GCTGCTGCACTTAAACTTCT+CGG | 0.428929 | 3.1:-90517810 | MS.gene37301:CDS |
| TGATGATAAGTCTTCAATAA+AGG | 0.429949 | 3.1:-90517289 | MS.gene37301:CDS |
| AACTTCCTTGAGGTTTCATC+CGG | 0.430850 | 3.1:-90517577 | MS.gene37301:CDS |
| TTTGGTATTCCTTTAAAAGC+TGG | 0.431430 | 3.1:-90517245 | MS.gene37301:CDS |
| GAGGTTTCATCCGGACAAAC+AGG | 0.431529 | 3.1:-90517568 | MS.gene37301:CDS |
| GAGATTGAGCTATTGAGAAA+AGG | 0.438019 | 3.1:-90516550 | MS.gene37301:CDS |
| ATCAAAAGCTTTGGCTGTAT+CGG | 0.439665 | 3.1:+90516408 | None:intergenic |
| CAAATATATGGCAAAGAAGT+TGG | 0.442593 | 3.1:-90517004 | MS.gene37301:CDS |
| AAGAAATGTATCAAAAGCTT+TGG | 0.451091 | 3.1:+90516399 | None:intergenic |
| ATTTGATATCGAACAATTGA+GGG | 0.453929 | 3.1:-90516761 | MS.gene37301:CDS |
| CATTTGATATCGAACAATTG+AGG | 0.454216 | 3.1:-90516762 | MS.gene37301:CDS |
| CCAAAGGAACTTCAAGGAGA+TGG | 0.456430 | 3.1:-90516513 | MS.gene37301:CDS |
| CTTCGCAGCTTCAGTTTGCT+CGG | 0.456856 | 3.1:+90517530 | None:intergenic |
| CCAACACTGCATAAGCCTCT+TGG | 0.462398 | 3.1:+90517466 | None:intergenic |
| GATGCAATTGATTGTGCTGC+AGG | 0.463598 | 3.1:+90516364 | None:intergenic |
| ACAGCAGCAGCCTGTTTGTC+CGG | 0.463811 | 3.1:+90517558 | None:intergenic |
| CAAGATCAAACTCATCAGCC+TGG | 0.467623 | 3.1:+90517211 | None:intergenic |
| AGAGGCGTGGTCTGCAGTGC+AGG | 0.469414 | 3.1:-90516158 | MS.gene37301:CDS |
| CTGGTTAGCTTCCTTTGGGA+AGG | 0.471179 | 3.1:+90516108 | None:intergenic |
| GTCGTCGTCATCGTCGGTGT+TGG | 0.475726 | 3.1:+90516253 | None:intergenic |
| CAAAGGAAGCTAACCAGAGA+TGG | 0.482494 | 3.1:-90516102 | MS.gene37301:CDS |
| ATTGTTGTCGTCGTCATCGT+CGG | 0.494599 | 3.1:+90516247 | None:intergenic |
| TTGATACATTTCTTGAGAAG+AGG | 0.495249 | 3.1:-90516390 | MS.gene37301:CDS |
| AGCTGCTTTGCAACAGAAGA+AGG | 0.496514 | 3.1:-90516908 | MS.gene37301:CDS |
| CACTGGATCACGGTCTTCCC+AGG | 0.497559 | 3.1:+90516058 | None:intergenic |
| TCAATCTCTTCTTTAGTCCA+AGG | 0.508908 | 3.1:+90516565 | None:intergenic |
| AGCAGGCTACACAGAGAAGA+AGG | 0.509420 | 3.1:-90516593 | MS.gene37301:CDS |
| CAGAGAAGAAGGAGAAACCT+TGG | 0.509658 | 3.1:-90516582 | MS.gene37301:CDS |
| AAGATCAAACTCATCAGCCT+GGG | 0.518267 | 3.1:+90517212 | None:intergenic |
| AATTCTTCTCTAGTTGACAA+TGG | 0.519138 | 3.1:+90516340 | None:intergenic |
| CCCTAGAATACTGAGAAGAA+AGG | 0.521673 | 3.1:-90517064 | MS.gene37301:CDS |
| CCGTAGCAAGATATCTAAGA+TGG | 0.529700 | 3.1:+90517628 | None:intergenic |
| CAAAGGAACTTCAAGGAGAT+GGG | 0.529793 | 3.1:-90516512 | MS.gene37301:CDS |
| GTTGCTGTCGGTACCGGTGT+TGG | 0.530623 | 3.1:+90516223 | None:intergenic |
| GTTTCAGAATACATTGGCAC+TGG | 0.531471 | 3.1:-90516484 | MS.gene37301:CDS |
| AATCAACCAATTCCATCTCT+AGG | 0.533477 | 3.1:-90517312 | MS.gene37301:CDS |
| GCATGAAAAGAGTGACCAGC+CGG | 0.535447 | 3.1:+90517831 | None:intergenic |
| GGAAGCAGTAGTTCTCTAGC+AGG | 0.540499 | 3.1:-90516610 | MS.gene37301:CDS |
| AGCGGCCAGAATTGCTGAAG+AGG | 0.545593 | 3.1:-90516971 | MS.gene37301:CDS |
| ATCATAAAAGGTGGATGGAA+AGG | 0.545814 | 3.1:-90517170 | MS.gene37301:CDS |
| TATGGCAAAGAAGTTGGAGG+AGG | 0.545831 | 3.1:-90516998 | MS.gene37301:CDS |
| AGTTTCCCAAAGGAACTTCA+AGG | 0.546208 | 3.1:-90516519 | MS.gene37301:CDS |
| CGAGCAAACTGAAGCTGCGA+AGG | 0.546255 | 3.1:-90517529 | MS.gene37301:CDS |
| CCGAGATCATAAAAGGTGGA+TGG | 0.548047 | 3.1:-90517175 | MS.gene37301:CDS |
| TTGATGCTGCTTGTTGCTGT+CGG | 0.552607 | 3.1:+90516211 | None:intergenic |
| CCAAGAGGCTTATGCAGTGT+TGG | 0.555210 | 3.1:-90517466 | MS.gene37301:CDS |
| GCAAACTGAAGCTGCGAAGG+AGG | 0.560752 | 3.1:-90517526 | MS.gene37301:CDS |
| AATACTGAGAAGAAAGGAAG+AGG | 0.562192 | 3.1:-90517058 | MS.gene37301:CDS |
| CGTCCATTCCTCATAAAAGC+AGG | 0.567375 | 3.1:+90517345 | None:intergenic |
| TGGCTGTATCGGGCTTCTGG+AGG | 0.570452 | 3.1:+90516419 | None:intergenic |
| GACTTATCATCACCTAGAGA+TGG | 0.570557 | 3.1:+90517300 | None:intergenic |
| TATGAGGAATGGACGGTGGT+CGG | 0.570845 | 3.1:-90517337 | MS.gene37301:CDS |
| CAACTAGAGAAGAATTGGAA+GGG | 0.572911 | 3.1:-90516333 | MS.gene37301:CDS |
| AACACCTTGTAGAAGTCTTG+TGG | 0.578361 | 3.1:+90517372 | None:intergenic |
| ATCGGGCTTCTGGAGGAGAA+CGG | 0.578804 | 3.1:+90516426 | None:intergenic |
| TGTTGATAATGCTTATAAGA+GGG | 0.580470 | 3.1:-90517088 | MS.gene37301:CDS |
| TCAAGGAGTTTCAGAACAAG+AGG | 0.581261 | 3.1:-90516176 | MS.gene37301:CDS |
| GAGGTTGTTTCAGAATACAT+TGG | 0.584103 | 3.1:-90516490 | MS.gene37301:CDS |
| GGAGAAACAACGAAAAGCAG+AGG | 0.585652 | 3.1:-90516950 | MS.gene37301:CDS |
| GAAGTTGGAGGAGGAAGAAG+CGG | 0.588565 | 3.1:-90516989 | MS.gene37301:CDS |
| ATATATGGCAAAGAAGTTGG+AGG | 0.590878 | 3.1:-90517001 | MS.gene37301:CDS |
| AAAGGAAGCTAACCAGAGAT+GGG | 0.594916 | 3.1:-90516101 | MS.gene37301:CDS |
| AGCAACAAGCAGCATCAACT+CGG | 0.597280 | 3.1:-90516206 | MS.gene37301:CDS |
| CCATCTTAGATATCTTGCTA+CGG | 0.601973 | 3.1:-90517628 | MS.gene37301:CDS |
| AGCAAGAAGAAATCTGGTGA+CGG | 0.602442 | 3.1:-90517693 | MS.gene37301:CDS |
| GAGTAGCTGCAGCTGTACCT+GGG | 0.604010 | 3.1:-90516075 | MS.gene37301:CDS |
| AGTATGTGTTGTGACAAGAT+AGG | 0.605889 | 3.1:+90516823 | None:intergenic |
| TCAACAAAATGGCTATGTCA+AGG | 0.605950 | 3.1:-90516638 | MS.gene37301:CDS |
| TCAACTAGAGAAGAATTGGA+AGG | 0.608211 | 3.1:-90516334 | MS.gene37301:CDS |
| AACTAGAGAAGAATTGGAAG+GGG | 0.608691 | 3.1:-90516332 | MS.gene37301:CDS |
| ACAGAAGAAGGTGAAAGAGA+AGG | 0.608855 | 3.1:-90516896 | MS.gene37301:CDS |
| GCTATCATAAAACTTCCTTG+AGG | 0.611239 | 3.1:-90517587 | MS.gene37301:CDS |
| ACTCACAAGAGCTTGTAGAT+GGG | 0.613229 | 3.1:-90517908 | MS.gene37301:CDS |
| CATGAAAAGAGTGACCAGCC+GGG | 0.617908 | 3.1:+90517832 | None:intergenic |
| CTTGTGAGTAAGTAATAAGA+CGG | 0.621140 | 3.1:+90517922 | None:intergenic |
| GTCTTGTGGATCACAGTCAG+TGG | 0.627262 | 3.1:+90517386 | None:intergenic |
| GGAATACAAAAGTTTCCCAA+AGG | 0.628434 | 3.1:-90516529 | MS.gene37301:CDS |
| TCTTGTGGATCACAGTCAGT+GGG | 0.629359 | 3.1:+90517387 | None:intergenic |
| TAAGGCGTTGAAATATGACC+CGG | 0.631520 | 3.1:-90517850 | MS.gene37301:CDS |
| AAGCTGGAGAGAGTTTCCCC+AGG | 0.640812 | 3.1:-90517229 | MS.gene37301:CDS |
| TTTGTCCGGATGAAACCTCA+AGG | 0.646808 | 3.1:+90517572 | None:intergenic |
| TTTGATATCGAACAATTGAG+GGG | 0.649974 | 3.1:-90516760 | MS.gene37301:CDS |
| GACGACAACAATTCCAACAC+CGG | 0.650077 | 3.1:-90516236 | MS.gene37301:CDS |
| ATCAACTCGGAAGATTCTCA+AGG | 0.650839 | 3.1:-90516193 | MS.gene37301:CDS |
| TCTTAGATATCTTGCTACGG+AGG | 0.651757 | 3.1:-90517625 | MS.gene37301:CDS |
| TGATCCACAAGACTTCTACA+AGG | 0.666734 | 3.1:-90517376 | MS.gene37301:CDS |
| AGATCAAACTCATCAGCCTG+GGG | 0.670566 | 3.1:+90517213 | None:intergenic |
| GGTTTGTGTGAGAAAATCGA+AGG | 0.671097 | 3.1:-90516739 | MS.gene37301:CDS |
| GCGTTGAAATATGACCCGGC+TGG | 0.672506 | 3.1:-90517846 | MS.gene37301:CDS |
| TGAGAAAATCGAAGGCAAAG+AGG | 0.673494 | 3.1:-90516731 | MS.gene37301:CDS |
| AATCCCGAGATCATAAAAGG+TGG | 0.674327 | 3.1:-90517179 | MS.gene37301:CDS |
| TTCTTCTCTAGTTGACAATG+GGG | 0.676675 | 3.1:+90516342 | None:intergenic |
| GAGTTTCAGAACAAGAGGCG+TGG | 0.676797 | 3.1:-90516171 | MS.gene37301:CDS |
| TTGCTGCTGTAAGAATCAGA+AGG | 0.678587 | 3.1:+90517717 | None:intergenic |
| AAGCATTATCAACAAGAGTG+CGG | 0.686922 | 3.1:+90517097 | None:intergenic |
| AGGAACTTCAAGGAGATGGG+AGG | 0.689319 | 3.1:-90516509 | MS.gene37301:CDS |
| AGATGCTGATAAGAAGAATG+TGG | 0.708564 | 3.1:-90517766 | MS.gene37301:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCATAAATTCTTCTTTTTA+CGG | + | chr3.1:90516508-90516527 | None:intergenic | 15.0% |
| !!! | TCATAAATTCTTCTTTTTAC+GGG | + | chr3.1:90516507-90516526 | None:intergenic | 20.0% |
| !!! | TGAAATAGATGTTCATTTTA+AGG | - | chr3.1:90516449-90516468 | MS.gene37301:CDS | 20.0% |
| !!! | TTGATAGTTTCTACAATTTT+TGG | - | chr3.1:90516679-90516698 | MS.gene37301:CDS | 20.0% |
| ! | AAGAAATGTATCAAAAGCTT+TGG | + | chr3.1:90517546-90517565 | None:intergenic | 25.0% |
| ! | ATTTGATATCGAACAATTGA+GGG | - | chr3.1:90517181-90517200 | MS.gene37301:CDS | 25.0% |
| ! | CAAGATCATTATGCATTATT+GGG | - | chr3.1:90516285-90516304 | MS.gene37301:CDS | 25.0% |
| ! | CAATTAGACGAAACAAAAAT+AGG | + | chr3.1:90516063-90516082 | None:intergenic | 25.0% |
| ! | GAGAAAAGTAATCAACAAAA+TGG | - | chr3.1:90517293-90517312 | MS.gene37301:CDS | 25.0% |
| ! | TGATGATAAGTCTTCAATAA+AGG | - | chr3.1:90516653-90516672 | MS.gene37301:CDS | 25.0% |
| !!! | TGTTGATAATGCTTATAAGA+GGG | - | chr3.1:90516854-90516873 | MS.gene37301:CDS | 25.0% |
| !!! | TTGTTGATAATGCTTATAAG+AGG | - | chr3.1:90516853-90516872 | MS.gene37301:CDS | 25.0% |
| AATTCTTCTCTAGTTGACAA+TGG | + | chr3.1:90517605-90517624 | None:intergenic | 30.0% | |
| ATTCTTCTCTAGTTGACAAT+GGG | + | chr3.1:90517604-90517623 | None:intergenic | 30.0% | |
| ATTGTCAACTAGAGAAGAAT+TGG | - | chr3.1:90517604-90517623 | MS.gene37301:CDS | 30.0% | |
| CAAATATATGGCAAAGAAGT+TGG | - | chr3.1:90516938-90516957 | MS.gene37301:CDS | 30.0% | |
| CAACTTCTTTGCCATATATT+TGG | + | chr3.1:90516940-90516959 | None:intergenic | 30.0% | |
| CATTTGATATCGAACAATTG+AGG | - | chr3.1:90517180-90517199 | MS.gene37301:CDS | 30.0% | |
| CTTGTGAGTAAGTAATAAGA+CGG | + | chr3.1:90516023-90516042 | None:intergenic | 30.0% | |
| GAAAAAATTTGCCATGATGA+AGG | - | chr3.1:90517910-90517929 | MS.gene37301:CDS | 30.0% | |
| GAAAAAGGAGTCCAAATATA+TGG | - | chr3.1:90516926-90516945 | MS.gene37301:CDS | 30.0% | |
| GCAAGATCATTATGCATTAT+TGG | - | chr3.1:90516284-90516303 | MS.gene37301:CDS | 30.0% | |
| TTGATACATTTCTTGAGAAG+AGG | - | chr3.1:90517552-90517571 | MS.gene37301:CDS | 30.0% | |
| TTTGATATCGAACAATTGAG+GGG | - | chr3.1:90517182-90517201 | MS.gene37301:CDS | 30.0% | |
| ! | TTTGGTATTCCTTTAAAAGC+TGG | - | chr3.1:90516697-90516716 | MS.gene37301:CDS | 30.0% |
| AACTAGAGAAGAATTGGAAG+GGG | - | chr3.1:90517610-90517629 | MS.gene37301:CDS | 35.0% | |
| AAGCATTATCAACAAGAGTG+CGG | + | chr3.1:90516848-90516867 | None:intergenic | 35.0% | |
| AATACTGAGAAGAAAGGAAG+AGG | - | chr3.1:90516884-90516903 | MS.gene37301:CDS | 35.0% | |
| AATCAACCAATTCCATCTCT+AGG | - | chr3.1:90516630-90516649 | MS.gene37301:CDS | 35.0% | |
| AGATGCTGATAAGAAGAATG+TGG | - | chr3.1:90516176-90516195 | MS.gene37301:CDS | 35.0% | |
| AGCTGAGAAGAAAAGGAAAA+AGG | - | chr3.1:90516911-90516930 | MS.gene37301:CDS | 35.0% | |
| AGTATGTGTTGTGACAAGAT+AGG | + | chr3.1:90517122-90517141 | None:intergenic | 35.0% | |
| ATATATGGCAAAGAAGTTGG+AGG | - | chr3.1:90516941-90516960 | MS.gene37301:CDS | 35.0% | |
| ATCATAAAAGGTGGATGGAA+AGG | - | chr3.1:90516772-90516791 | MS.gene37301:CDS | 35.0% | |
| CAAAACTGTCAGAAAAAGCT+AGG | - | chr3.1:90516802-90516821 | MS.gene37301:CDS | 35.0% | |
| CAACTAGAGAAGAATTGGAA+GGG | - | chr3.1:90517609-90517628 | MS.gene37301:CDS | 35.0% | |
| CCATCTTAGATATCTTGCTA+CGG | - | chr3.1:90516314-90516333 | MS.gene37301:CDS | 35.0% | |
| CCATGATGAAGGAAAATTTC+AGG | - | chr3.1:90517921-90517940 | MS.gene37301:CDS | 35.0% | |
| CCTTTCTTCTCAGTATTCTA+GGG | + | chr3.1:90516881-90516900 | None:intergenic | 35.0% | |
| GAAGAATGTGGTTGAAGATA+AGG | - | chr3.1:90516188-90516207 | MS.gene37301:CDS | 35.0% | |
| GAGATTGAGCTATTGAGAAA+AGG | - | chr3.1:90517392-90517411 | MS.gene37301:CDS | 35.0% | |
| GCTATCATAAAACTTCCTTG+AGG | - | chr3.1:90516355-90516374 | MS.gene37301:CDS | 35.0% | |
| GGAATACAAAAGTTTCCCAA+AGG | - | chr3.1:90517413-90517432 | MS.gene37301:CDS | 35.0% | |
| TCAACAAAATGGCTATGTCA+AGG | - | chr3.1:90517304-90517323 | MS.gene37301:CDS | 35.0% | |
| TCAACTAGAGAAGAATTGGA+AGG | - | chr3.1:90517608-90517627 | MS.gene37301:CDS | 35.0% | |
| TCAATCTCTTCTTTAGTCCA+AGG | + | chr3.1:90517380-90517399 | None:intergenic | 35.0% | |
| TCATATTTCAACGCCTTAAC+CGG | + | chr3.1:90516090-90516109 | None:intergenic | 35.0% | |
| TCCTTTCTTCTCAGTATTCT+AGG | + | chr3.1:90516882-90516901 | None:intergenic | 35.0% | |
| TGTTTCGTCTAATTGTCTTC+CGG | - | chr3.1:90516068-90516087 | MS.gene37301:CDS | 35.0% | |
| TTCTTCTCTAGTTGACAATG+GGG | + | chr3.1:90517603-90517622 | None:intergenic | 35.0% | |
| ! | AAACTCTCTCCAGCTTTTAA+AGG | + | chr3.1:90516709-90516728 | None:intergenic | 35.0% |
| ! | ATCAAAAGCTTTGGCTGTAT+CGG | + | chr3.1:90517537-90517556 | None:intergenic | 35.0% |
| ! | CCTGAAATTTTCCTTCATCA+TGG | + | chr3.1:90517924-90517943 | None:intergenic | 35.0% |
| ! | GAGGTTGTTTCAGAATACAT+TGG | - | chr3.1:90517452-90517471 | MS.gene37301:CDS | 35.0% |
| !! | AGCTTTAAAAACCTTCCCAA+AGG | - | chr3.1:90517823-90517842 | MS.gene37301:CDS | 35.0% |
| !! | CTATTTTCAGTTGTTGTTGC+TGG | + | chr3.1:90517644-90517663 | None:intergenic | 35.0% |
| !!! | TCATTTTAAGGCAATCCAAG+AGG | - | chr3.1:90516461-90516480 | MS.gene37301:CDS | 35.0% |
| !!! | TGGCAAATTTTTTCTTGCAC+TGG | + | chr3.1:90517904-90517923 | None:intergenic | 35.0% |
| !!! | TTTTTTCTTGCACTGGATCA+CGG | + | chr3.1:90517897-90517916 | None:intergenic | 35.0% |
| AAAGGAAGCTAACCAGAGAT+GGG | - | chr3.1:90517841-90517860 | MS.gene37301:CDS | 40.0% | |
| AACACCTTGTAGAAGTCTTG+TGG | + | chr3.1:90516573-90516592 | None:intergenic | 40.0% | |
| AACTTCCTTGAGGTTTCATC+CGG | - | chr3.1:90516365-90516384 | MS.gene37301:CDS | 40.0% | |
| AAGATCAAACTCATCAGCCT+GGG | + | chr3.1:90516733-90516752 | None:intergenic | 40.0% | |
| AATCCCGAGATCATAAAAGG+TGG | - | chr3.1:90516763-90516782 | MS.gene37301:CDS | 40.0% | |
| AATGGCTATGTCAAGGCTAA+TGG | - | chr3.1:90517311-90517330 | MS.gene37301:CDS | 40.0% | |
| ACAGAAGAAGGTGAAAGAGA+AGG | - | chr3.1:90517046-90517065 | MS.gene37301:CDS | 40.0% | |
| ACTCACAAGAGCTTGTAGAT+GGG | - | chr3.1:90516034-90516053 | MS.gene37301:CDS | 40.0% | |
| ACTGAGTTGCAAGAAAAACG+TGG | - | chr3.1:90517265-90517284 | MS.gene37301:CDS | 40.0% | |
| ACTGTCAGAAAAAGCTAGGA+AGG | - | chr3.1:90516806-90516825 | MS.gene37301:CDS | 40.0% | |
| AGAAGGAGAAAAAGCTCTTG+CGG | - | chr3.1:90517063-90517082 | MS.gene37301:CDS | 40.0% | |
| AGCAAGAAGAAATCTGGTGA+CGG | - | chr3.1:90516249-90516268 | MS.gene37301:CDS | 40.0% | |
| AGCAAGAGCAAGAAGAAATC+TGG | - | chr3.1:90516243-90516262 | MS.gene37301:CDS | 40.0% | |
| AGGCAAAAGCTGAGAAGAAA+AGG | - | chr3.1:90516904-90516923 | MS.gene37301:CDS | 40.0% | |
| AGTTTCCCAAAGGAACTTCA+AGG | - | chr3.1:90517423-90517442 | MS.gene37301:CDS | 40.0% | |
| ATCAACTCGGAAGATTCTCA+AGG | - | chr3.1:90517749-90517768 | MS.gene37301:CDS | 40.0% | |
| CAAAGGAACTTCAAGGAGAT+GGG | - | chr3.1:90517430-90517449 | MS.gene37301:CDS | 40.0% | |
| CATCTCCTTGAAGTTCCTTT+GGG | + | chr3.1:90517431-90517450 | None:intergenic | 40.0% | |
| CCCTAGAATACTGAGAAGAA+AGG | - | chr3.1:90516878-90516897 | MS.gene37301:CDS | 40.0% | |
| CCGTAGCAAGATATCTAAGA+TGG | + | chr3.1:90516317-90516336 | None:intergenic | 40.0% | |
| CTGAATCCCGAGATCATAAA+AGG | - | chr3.1:90516760-90516779 | MS.gene37301:CDS | 40.0% | |
| GACTTATCATCACCTAGAGA+TGG | + | chr3.1:90516645-90516664 | None:intergenic | 40.0% | |
| GGTTTGTGTGAGAAAATCGA+AGG | - | chr3.1:90517203-90517222 | MS.gene37301:CDS | 40.0% | |
| GTCTAATTGTCTTCCGGTTA+AGG | - | chr3.1:90516074-90516093 | MS.gene37301:CDS | 40.0% | |
| GTTTCAGAATACATTGGCAC+TGG | - | chr3.1:90517458-90517477 | MS.gene37301:CDS | 40.0% | |
| TACTCACAAGAGCTTGTAGA+TGG | - | chr3.1:90516033-90516052 | MS.gene37301:CDS | 40.0% | |
| TCAAGGAGTTTCAGAACAAG+AGG | - | chr3.1:90517766-90517785 | MS.gene37301:CDS | 40.0% | |
| TCATCACCTAGAGATGGAAT+TGG | + | chr3.1:90516639-90516658 | None:intergenic | 40.0% | |
| TCTTAGATATCTTGCTACGG+AGG | - | chr3.1:90516317-90516336 | MS.gene37301:CDS | 40.0% | |
| TGAGAAAATCGAAGGCAAAG+AGG | - | chr3.1:90517211-90517230 | MS.gene37301:CDS | 40.0% | |
| TGATCCACAAGACTTCTACA+AGG | - | chr3.1:90516566-90516585 | MS.gene37301:CDS | 40.0% | |
| TTGCTGCTGTAAGAATCAGA+AGG | + | chr3.1:90516228-90516247 | None:intergenic | 40.0% | |
| TTTCTCCTCTTCAGCAATTC+TGG | + | chr3.1:90516979-90516998 | None:intergenic | 40.0% | |
| ! | CAAGACTTCTACAAGGTGTT+TGG | - | chr3.1:90516573-90516592 | MS.gene37301:CDS | 40.0% |
| ! | CATCCACCTTTTATGATCTC+GGG | + | chr3.1:90516769-90516788 | None:intergenic | 40.0% |
| ! | CCATCCACCTTTTATGATCT+CGG | + | chr3.1:90516770-90516789 | None:intergenic | 40.0% |
| ! | TCAAAAGCTTTGGCTGTATC+GGG | + | chr3.1:90517536-90517555 | None:intergenic | 40.0% |
| !! | ATCTCTGGTTAGCTTCCTTT+GGG | + | chr3.1:90517841-90517860 | None:intergenic | 40.0% |
| !! | CTGCTTTTATGAGGAATGGA+CGG | - | chr3.1:90516598-90516617 | MS.gene37301:CDS | 40.0% |
| !! | TAAGGCGTTGAAATATGACC+CGG | - | chr3.1:90516092-90516111 | MS.gene37301:CDS | 40.0% |
| !! | TGTTTGGTCCTGCTTTTATG+AGG | - | chr3.1:90516589-90516608 | MS.gene37301:CDS | 40.0% |
| AGATCAAACTCATCAGCCTG+GGG | + | chr3.1:90516732-90516751 | None:intergenic | 45.0% | |
| AGCAACAAGCAGCATCAACT+CGG | - | chr3.1:90517736-90517755 | MS.gene37301:CDS | 45.0% | |
| ATTGTTGTCGTCGTCATCGT+CGG | + | chr3.1:90517698-90517717 | None:intergenic | 45.0% | |
| CAAAGGAAGCTAACCAGAGA+TGG | - | chr3.1:90517840-90517859 | MS.gene37301:CDS | 45.0% | |
| CAAGATCAAACTCATCAGCC+TGG | + | chr3.1:90516734-90516753 | None:intergenic | 45.0% | |
| CAGAGAAGAAGGAGAAACCT+TGG | - | chr3.1:90517360-90517379 | MS.gene37301:CDS | 45.0% | |
| CCAAAGGAACTTCAAGGAGA+TGG | - | chr3.1:90517429-90517448 | MS.gene37301:CDS | 45.0% | |
| CCATCTCCTTGAAGTTCCTT+TGG | + | chr3.1:90517432-90517451 | None:intergenic | 45.0% | |
| CCGAGATCATAAAAGGTGGA+TGG | - | chr3.1:90516767-90516786 | MS.gene37301:CDS | 45.0% | |
| CGTCCATTCCTCATAAAAGC+AGG | + | chr3.1:90516600-90516619 | None:intergenic | 45.0% | |
| CTTCTGTTGCAAAGCAGCTT+CGG | + | chr3.1:90517033-90517052 | None:intergenic | 45.0% | |
| GACGACAACAATTCCAACAC+CGG | - | chr3.1:90517706-90517725 | MS.gene37301:CDS | 45.0% | |
| GATGCAATTGATTGTGCTGC+AGG | + | chr3.1:90517581-90517600 | None:intergenic | 45.0% | |
| GCTGCTGCACTTAAACTTCT+CGG | - | chr3.1:90516132-90516151 | MS.gene37301:CDS | 45.0% | |
| GGAGAAACAACGAAAAGCAG+AGG | - | chr3.1:90516992-90517011 | MS.gene37301:CDS | 45.0% | |
| TATGGCAAAGAAGTTGGAGG+AGG | - | chr3.1:90516944-90516963 | MS.gene37301:CDS | 45.0% | |
| TCTTGTGGATCACAGTCAGT+GGG | + | chr3.1:90516558-90516577 | None:intergenic | 45.0% | |
| TTTGTCCGGATGAAACCTCA+AGG | + | chr3.1:90516373-90516392 | None:intergenic | 45.0% | |
| ! | AGCTGCTTTGCAACAGAAGA+AGG | - | chr3.1:90517034-90517053 | MS.gene37301:CDS | 45.0% |
| ! | CTTTTATGAGGAATGGACGG+TGG | - | chr3.1:90516601-90516620 | MS.gene37301:CDS | 45.0% |
| ! | TTGATGCTGCTTGTTGCTGT+CGG | + | chr3.1:90517734-90517753 | None:intergenic | 45.0% |
| !! | CATCTCTGGTTAGCTTCCTT+TGG | + | chr3.1:90517842-90517861 | None:intergenic | 45.0% |
| !! | GGTCCTGCTTTTATGAGGAA+TGG | - | chr3.1:90516594-90516613 | MS.gene37301:CDS | 45.0% |
| AGCAGGCTACACAGAGAAGA+AGG | - | chr3.1:90517349-90517368 | MS.gene37301:CDS | 50.0% | |
| AGGAACTTCAAGGAGATGGG+AGG | - | chr3.1:90517433-90517452 | MS.gene37301:CDS | 50.0% | |
| CATGAAAAGAGTGACCAGCC+GGG | + | chr3.1:90516113-90516132 | None:intergenic | 50.0% | |
| CCAACACTGCATAAGCCTCT+TGG | + | chr3.1:90516479-90516498 | None:intergenic | 50.0% | |
| CCAAGAGGCTTATGCAGTGT+TGG | - | chr3.1:90516476-90516495 | MS.gene37301:CDS | 50.0% | |
| GAAGTTGGAGGAGGAAGAAG+CGG | - | chr3.1:90516953-90516972 | MS.gene37301:CDS | 50.0% | |
| GAGGTTTCATCCGGACAAAC+AGG | - | chr3.1:90516374-90516393 | MS.gene37301:CDS | 50.0% | |
| GAGTTTCAGAACAAGAGGCG+TGG | - | chr3.1:90517771-90517790 | MS.gene37301:CDS | 50.0% | |
| GCATGAAAAGAGTGACCAGC+CGG | + | chr3.1:90516114-90516133 | None:intergenic | 50.0% | |
| GGAAGCAGTAGTTCTCTAGC+AGG | - | chr3.1:90517332-90517351 | MS.gene37301:CDS | 50.0% | |
| GGAGAAAAAGCTCTTGCGGA+AGG | - | chr3.1:90517067-90517086 | MS.gene37301:CDS | 50.0% | |
| GTCTTGTGGATCACAGTCAG+TGG | + | chr3.1:90516559-90516578 | None:intergenic | 50.0% | |
| TATGAGGAATGGACGGTGGT+CGG | - | chr3.1:90516605-90516624 | MS.gene37301:CDS | 50.0% | |
| ! | CTTCGCAGCTTCAGTTTGCT+CGG | + | chr3.1:90516415-90516434 | None:intergenic | 50.0% |
| !! | CTGGTTAGCTTCCTTTGGGA+AGG | + | chr3.1:90517837-90517856 | None:intergenic | 50.0% |
| AAGCTGGAGAGAGTTTCCCC+AGG | - | chr3.1:90516713-90516732 | MS.gene37301:CDS | 55.0% | |
| ACAGCAGCAGCCTGTTTGTC+CGG | + | chr3.1:90516387-90516406 | None:intergenic | 55.0% | |
| AGCGGCCAGAATTGCTGAAG+AGG | - | chr3.1:90516971-90516990 | MS.gene37301:CDS | 55.0% | |
| CAGCTACTCGTTCCCATCTC+TGG | + | chr3.1:90517856-90517875 | None:intergenic | 55.0% | |
| CGAGCAAACTGAAGCTGCGA+AGG | - | chr3.1:90516413-90516432 | MS.gene37301:CDS | 55.0% | |
| CTTTGGCTGTATCGGGCTTC+TGG | + | chr3.1:90517529-90517548 | None:intergenic | 55.0% | |
| GAGTAGCTGCAGCTGTACCT+GGG | - | chr3.1:90517867-90517886 | MS.gene37301:CDS | 55.0% | |
| GCAAACTGAAGCTGCGAAGG+AGG | - | chr3.1:90516416-90516435 | MS.gene37301:CDS | 55.0% | |
| GCGTTGAAATATGACCCGGC+TGG | - | chr3.1:90516096-90516115 | MS.gene37301:CDS | 55.0% | |
| ! | ATCGGGCTTCTGGAGGAGAA+CGG | + | chr3.1:90517519-90517538 | None:intergenic | 55.0% |
| ! | CTGCTTGTTGCTGTCGGTAC+CGG | + | chr3.1:90517728-90517747 | None:intergenic | 55.0% |
| CACTGGATCACGGTCTTCCC+AGG | + | chr3.1:90517887-90517906 | None:intergenic | 60.0% | |
| CGAGTAGCTGCAGCTGTACC+TGG | - | chr3.1:90517866-90517885 | MS.gene37301:CDS | 60.0% | |
| GTCGTCGTCATCGTCGGTGT+TGG | + | chr3.1:90517692-90517711 | None:intergenic | 60.0% | |
| TGGCTGTATCGGGCTTCTGG+AGG | + | chr3.1:90517526-90517545 | None:intergenic | 60.0% | |
| !! | GTTGCTGTCGGTACCGGTGT+TGG | + | chr3.1:90517722-90517741 | None:intergenic | 60.0% |
| ! | AGAGGCGTGGTCTGCAGTGC+AGG | - | chr3.1:90517784-90517803 | MS.gene37301:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 90516000 | 90517964 | 90516000 | ID=MS.gene37301 |
| chr3.1 | mRNA | 90516000 | 90517964 | 90516000 | ID=MS.gene37301.t1;Parent=MS.gene37301 |
| chr3.1 | exon | 90516000 | 90517964 | 90516000 | ID=MS.gene37301.t1.exon1;Parent=MS.gene37301.t1 |
| chr3.1 | CDS | 90516000 | 90517964 | 90516000 | ID=cds.MS.gene37301.t1;Parent=MS.gene37301.t1 |
| Gene Sequence |
| Protein sequence |
