AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40860


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40860.t1 MTR_5g044050 98.020 101 2 0 25 125 57 157 3.68e-64 199
MS.gene40860.t1 MTR_1g115505 46.535 101 50 3 26 125 117 214 8.13e-22 89.7
MS.gene40860.t1 MTR_8g071110 44.554 101 53 3 26 125 93 191 1.87e-19 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40860.t1 AT1G26520 91.000 100 9 0 26 125 70 169 5.16e-60 189
MS.gene40860.t1 AT1G15730 47.525 101 49 3 26 125 120 217 1.96e-23 94.4
MS.gene40860.t1 AT1G80480 48.515 101 48 3 26 125 117 214 3.72e-23 93.6

Find 35 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCTTTGGCATCTATTCTT+TGG 0.207860 5.2:+36082092 MS.gene40860:CDS
AATGAGTTTGGTGAGGAAAT+TGG 0.300166 5.2:+36081404 MS.gene40860:CDS
GTCACCGTAAGTATCGTTAA+TGG 0.320808 5.2:+36082157 MS.gene40860:CDS
TTGCACTGTTAAACATAGTT+TGG 0.335183 5.2:+36081508 MS.gene40860:CDS
AACAGAAGATTGTTGAGAAA+TGG 0.344084 5.2:-36080587 None:intergenic
TTTGGCTAATCCAGCACCTT+TGG 0.352272 5.2:+36082078 MS.gene40860:CDS
GCTGTGATATTGAATGAGTT+TGG 0.371555 5.2:+36081392 MS.gene40860:CDS
ACTCTCATCACCGGTTATCT+TGG 0.407030 5.2:+36080616 MS.gene40860:CDS
TTGGCATCTATTCTTTGGTT+GGG 0.407476 5.2:+36082097 MS.gene40860:CDS
TTTGGCATCTATTCTTTGGT+TGG 0.414271 5.2:+36082096 MS.gene40860:CDS
TTCTCTGCACAAGCTGCTCA+AGG 0.418476 5.2:-36081538 None:intergenic
AGAATAGATGCCAAAGGTGC+TGG 0.420889 5.2:-36082088 None:intergenic
ATTGCTGGAAACTACTGGTT+TGG 0.422220 5.2:+36082060 MS.gene40860:CDS
CAGTAGTTTCCAGCAATATA+TGG 0.429786 5.2:-36082054 None:intergenic
TAGGCTAGACCATATATTGC+TGG 0.449889 5.2:+36082045 MS.gene40860:intron
GAAAGAGCATTGATAAATGA+AGG 0.462576 5.2:+36081431 MS.gene40860:CDS
TTTGCCAGCACCAAGATAAC+CGG 0.477454 5.2:-36080626 None:intergenic
GTGGTGCAGTTGTTGAAGAA+TGG 0.477782 5.2:+36081459 MS.gene40860:CDS
CATATATTGCTGGAAACTAC+TGG 0.482344 5.2:+36082055 MS.gene40860:CDS
TGGTGCAGTTGTTGAAGAAT+GGG 0.484968 5.2:+36081460 MS.gene40860:CDS
TCATCCATTAACGATACTTA+CGG 0.485998 5.2:-36082161 None:intergenic
TCTTTGGTTGGGTGAACAGT+TGG 0.490349 5.2:+36082108 MS.gene40860:CDS
GAATGGGTTGAACTTGCTAA+TGG 0.500533 5.2:+36081476 MS.gene40860:CDS
ATTTCTCAACAATCTTCTGT+TGG 0.503553 5.2:+36080589 MS.gene40860:CDS
ATCACCGGTTATCTTGGTGC+TGG 0.534620 5.2:+36080622 MS.gene40860:CDS
TCTCTGCACAAGCTGCTCAA+GGG 0.549355 5.2:-36081537 None:intergenic
TTGATAAATGAAGGGGAAAG+TGG 0.553774 5.2:+36081440 MS.gene40860:CDS
GTTGGTGTCACTCTCATCAC+CGG 0.555661 5.2:+36080607 MS.gene40860:CDS
AAAGAGCATTGATAAATGAA+GGG 0.594588 5.2:+36081432 MS.gene40860:CDS
AAGAGCATTGATAAATGAAG+GGG 0.611247 5.2:+36081433 MS.gene40860:CDS
TCTTGGTGCTGGCAAATCCA+CGG 0.620578 5.2:+36080633 MS.gene40860:CDS
AGCTTGTGCAGAGAAAAGAA+AGG 0.645456 5.2:+36081546 MS.gene40860:CDS
GTTTGGTGAGGAAATTGGAG+TGG 0.654078 5.2:+36081409 MS.gene40860:CDS
AACCAAAGAATAGATGCCAA+AGG 0.667612 5.2:-36082094 None:intergenic
GATATTGAATGAGTTTGGTG+AGG 0.685395 5.2:+36081397 MS.gene40860:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAATCTTAG+AGG + chr5.2:36081189-36081208 MS.gene40860:intron 10.0%
!! AAAAAAAAAAAAATCTTAGA+GGG + chr5.2:36081190-36081209 MS.gene40860:intron 10.0%
!!! TTTCATTTTATTTTTGTTAT+TGG + chr5.2:36080722-36080741 MS.gene40860:intron 10.0%
!! TATTTATAAGAGATTCAAAA+GGG - chr5.2:36081958-36081977 None:intergenic 15.0%
!! TGTTTAATTGTACTAAAATA+CGG - chr5.2:36082004-36082023 None:intergenic 15.0%
!! TTATATACATAAGCAATAAT+AGG - chr5.2:36081695-36081714 None:intergenic 15.0%
!!! AAGTATTTTTTATTTGGATT+TGG + chr5.2:36080876-36080895 MS.gene40860:intron 15.0%
!!! TAGTACAATTAAACATTTTT+AGG + chr5.2:36082009-36082028 MS.gene40860:intron 15.0%
!! ATTTATAAGAGATTCAAAAG+GGG - chr5.2:36081957-36081976 None:intergenic 20.0%
!! CCAATTAGAAATTATAGAAA+GGG - chr5.2:36080675-36080694 None:intergenic 20.0%
!! CCTTTCTATAATTTCTAATT+GGG + chr5.2:36080673-36080692 MS.gene40860:intron 20.0%
!! CTATTTATAAGAGATTCAAA+AGG - chr5.2:36081959-36081978 None:intergenic 20.0%
!! TATAGAAAGGGAAAATTAAT+GGG - chr5.2:36080663-36080682 None:intergenic 20.0%
!! TTATAGAAAGGGAAAATTAA+TGG - chr5.2:36080664-36080683 None:intergenic 20.0%
!!! AACAAACTATTCAAATTTTG+AGG + chr5.2:36081579-36081598 MS.gene40860:intron 20.0%
!!! TCGTGTAAGTATTTTTTATT+TGG + chr5.2:36080870-36080889 MS.gene40860:intron 20.0%
! ACATAAGCAATAATAGGATA+AGG - chr5.2:36081689-36081708 None:intergenic 25.0%
! ATAGAAAGGGAAAATTAATG+GGG - chr5.2:36080662-36080681 None:intergenic 25.0%
! ATATATTGAACTCTCAACAT+GGG + chr5.2:36081360-36081379 MS.gene40860:intron 25.0%
! ATTCCATTTCACAAAATCAT+TGG - chr5.2:36081304-36081323 None:intergenic 25.0%
! CATAAGCAATAATAGGATAA+GGG - chr5.2:36081688-36081707 None:intergenic 25.0%
! CCAAAAACACTATAATTGTA+AGG + chr5.2:36081220-36081239 MS.gene40860:intron 25.0%
! CCCAATTAGAAATTATAGAA+AGG - chr5.2:36080676-36080695 None:intergenic 25.0%
! CCCTTTCTATAATTTCTAAT+TGG + chr5.2:36080672-36080691 MS.gene40860:intron 25.0%
! CTATTATTTCAACAAGCTAT+AGG - chr5.2:36080762-36080781 None:intergenic 25.0%
! TTATAAATAGATGCAGTGTT+CGG + chr5.2:36081969-36081988 MS.gene40860:intron 25.0%
!! AATATTTTCAGTAGCGTTTT+TGG + chr5.2:36080840-36080859 MS.gene40860:intron 25.0%
!! CCTTACAATTATAGTGTTTT+TGG - chr5.2:36081223-36081242 None:intergenic 25.0%
!! TTTAGGTATTTTGTGTATGT+AGG + chr5.2:36082026-36082045 MS.gene40860:intron 25.0%
!! TTTTCATATAAGCTATAAGC+TGG + chr5.2:36081751-36081770 MS.gene40860:intron 25.0%
!!! AAAGAGCATTGATAAATGAA+GGG + chr5.2:36081432-36081451 MS.gene40860:CDS 25.0%
!!! ATTTATTTCTATAAGCTCTC+CGG - chr5.2:36081791-36081810 None:intergenic 25.0%
AAACCTTGTAACTGTTATAC+TGG + chr5.2:36081258-36081277 MS.gene40860:intron 30.0%
AACAGAAGATTGTTGAGAAA+TGG - chr5.2:36080590-36080609 None:intergenic 30.0%
AATCCTTGATTTCAGCTTAT+TGG + chr5.2:36080929-36080948 MS.gene40860:intron 30.0%
ATCCTTGATTTCAGCTTATT+GGG + chr5.2:36080930-36080949 MS.gene40860:intron 30.0%
ATTTCTCAACAATCTTCTGT+TGG + chr5.2:36080589-36080608 MS.gene40860:CDS 30.0%
CATATATTGAACTCTCAACA+TGG + chr5.2:36081359-36081378 MS.gene40860:intron 30.0%
GAAAACTCACATACAAGTTA+GGG - chr5.2:36081122-36081141 None:intergenic 30.0%
GGCACTAAAAATTTAAACAC+CGG + chr5.2:36081600-36081619 MS.gene40860:intron 30.0%
TAGAAAATGCCGAAATTGTT+AGG + chr5.2:36081075-36081094 MS.gene40860:intron 30.0%
TCATCCATTAACGATACTTA+CGG - chr5.2:36082164-36082183 None:intergenic 30.0%
TGAAAACTCACATACAAGTT+AGG - chr5.2:36081123-36081142 None:intergenic 30.0%
TGTGAAATGGAATTGTGAAT+TGG + chr5.2:36081311-36081330 MS.gene40860:intron 30.0%
TTGCACTGTTAAACATAGTT+TGG + chr5.2:36081508-36081527 MS.gene40860:CDS 30.0%
! TTTTTGGATGATAGATGAAG+TGG - chr5.2:36081157-36081176 None:intergenic 30.0%
!! AAGAGCATTGATAAATGAAG+GGG + chr5.2:36081433-36081452 MS.gene40860:CDS 30.0%
!! GAAAGAGCATTGATAAATGA+AGG + chr5.2:36081431-36081450 MS.gene40860:CDS 30.0%
!! TTTATTTCTATAAGCTCTCC+GGG - chr5.2:36081790-36081809 None:intergenic 30.0%
AACCAAAGAATAGATGCCAA+AGG - chr5.2:36082097-36082116 None:intergenic 35.0%
AAGCACTTGTGAGATTCTTA+GGG - chr5.2:36081859-36081878 None:intergenic 35.0%
CAGTAGTTTCCAGCAATATA+TGG - chr5.2:36082057-36082076 None:intergenic 35.0%
CATATATTGCTGGAAACTAC+TGG + chr5.2:36082055-36082074 MS.gene40860:CDS 35.0%
CTCAATATAATCACGACACA+CGG - chr5.2:36080963-36080982 None:intergenic 35.0%
CTTCCAGTATAACAGTTACA+AGG - chr5.2:36081264-36081283 None:intergenic 35.0%
GCTGTGATATTGAATGAGTT+TGG + chr5.2:36081392-36081411 MS.gene40860:CDS 35.0%
TAAGCACTTGTGAGATTCTT+AGG - chr5.2:36081860-36081879 None:intergenic 35.0%
TTGATAAATGAAGGGGAAAG+TGG + chr5.2:36081440-36081459 MS.gene40860:CDS 35.0%
TTGGCATCTATTCTTTGGTT+GGG + chr5.2:36082097-36082116 MS.gene40860:CDS 35.0%
TTTGGCATCTATTCTTTGGT+TGG + chr5.2:36082096-36082115 MS.gene40860:CDS 35.0%
! AATGAGTTTGGTGAGGAAAT+TGG + chr5.2:36081404-36081423 MS.gene40860:CDS 35.0%
! GATATTGAATGAGTTTGGTG+AGG + chr5.2:36081397-36081416 MS.gene40860:CDS 35.0%
!! CTTGTATGTGAGTTTTCAAG+TGG + chr5.2:36081125-36081144 MS.gene40860:intron 35.0%
ACCGGACTCATAATTACACT+TGG + chr5.2:36081618-36081637 MS.gene40860:intron 40.0%
AGATTCTTAGGGAGAGCTTA+TGG - chr5.2:36081848-36081867 None:intergenic 40.0%
AGCTTGTGCAGAGAAAAGAA+AGG + chr5.2:36081546-36081565 MS.gene40860:CDS 40.0%
CACCTTTGGCATCTATTCTT+TGG + chr5.2:36082092-36082111 MS.gene40860:CDS 40.0%
CCCAAGTGTAATTATGAGTC+CGG - chr5.2:36081622-36081641 None:intergenic 40.0%
CCGGACTCATAATTACACTT+GGG + chr5.2:36081619-36081638 MS.gene40860:intron 40.0%
CTCACAAGTGCTTATACTAG+TGG + chr5.2:36081866-36081885 MS.gene40860:intron 40.0%
GAAAATTAATGGGGTTACCG+TGG - chr5.2:36080653-36080672 None:intergenic 40.0%
GATTTCAGCTTATTGGGCAT+TGG + chr5.2:36080936-36080955 MS.gene40860:intron 40.0%
GGATTAAAAGCAACCGAGTT+TGG - chr5.2:36080914-36080933 None:intergenic 40.0%
GGCATTCAGCCTAACAATTT+CGG - chr5.2:36081087-36081106 None:intergenic 40.0%
GTTTCTGCAAACTCCAAACT+CGG + chr5.2:36080898-36080917 MS.gene40860:intron 40.0%
TAGGCTAGACCATATATTGC+TGG + chr5.2:36082045-36082064 MS.gene40860:intron 40.0%
TCAGATAAGCTAATCCAAGC+AGG + chr5.2:36081895-36081914 MS.gene40860:intron 40.0%
TCTAGTTGAGCTCGAACTTA+TGG - chr5.2:36081059-36081078 None:intergenic 40.0%
TGCCCAATAAGCTGAAATCA+AGG - chr5.2:36080935-36080954 None:intergenic 40.0%
TGGTGCAGTTGTTGAAGAAT+GGG + chr5.2:36081460-36081479 MS.gene40860:CDS 40.0%
TTCACAAAATCATTGGCCGA+GGG - chr5.2:36081297-36081316 None:intergenic 40.0%
TTTCACAAAATCATTGGCCG+AGG - chr5.2:36081298-36081317 None:intergenic 40.0%
! ATTGCTGGAAACTACTGGTT+TGG + chr5.2:36082060-36082079 MS.gene40860:CDS 40.0%
! CGGCCAATGATTTTGTGAAA+TGG + chr5.2:36081298-36081317 MS.gene40860:intron 40.0%
! GGGGCTTTGATAAAAGTGAA+CGG - chr5.2:36081938-36081957 None:intergenic 40.0%
! GTCACCGTAAGTATCGTTAA+TGG + chr5.2:36082157-36082176 MS.gene40860:CDS 40.0%
!! GAATGGGTTGAACTTGCTAA+TGG + chr5.2:36081476-36081495 MS.gene40860:CDS 40.0%
ACATACAAGTTAGGGATCCC+AGG - chr5.2:36081114-36081133 None:intergenic 45.0%
GTGGTGCAGTTGTTGAAGAA+TGG + chr5.2:36081459-36081478 MS.gene40860:CDS 45.0%
TCTTTGGTTGGGTGAACAGT+TGG + chr5.2:36082108-36082127 MS.gene40860:CDS 45.0%
TGAACTCTCAACATGGGAAG+AGG + chr5.2:36081366-36081385 MS.gene40860:intron 45.0%
TTTGCCAGCACCAAGATAAC+CGG - chr5.2:36080629-36080648 None:intergenic 45.0%
TTTGGCTAATCCAGCACCTT+TGG + chr5.2:36082078-36082097 MS.gene40860:CDS 45.0%
! ACTCTCATCACCGGTTATCT+TGG + chr5.2:36080616-36080635 MS.gene40860:CDS 45.0%
! AGAATAGATGCCAAAGGTGC+TGG - chr5.2:36082091-36082110 None:intergenic 45.0%
! GCTGGTTTCATAAACTTGCC+CGG + chr5.2:36081769-36081788 MS.gene40860:intron 45.0%
! GTTTGGTGAGGAAATTGGAG+TGG + chr5.2:36081409-36081428 MS.gene40860:CDS 45.0%
!!! TTTTTTTTAGATTTTATTTT+TGG - chr5.2:36081173-36081192 None:intergenic 5.0%
AAGTTAGGGATCCCAGGTCA+TGG - chr5.2:36081108-36081127 None:intergenic 50.0%
AGATGCAGTGTTCGGCAAAG+TGG + chr5.2:36081977-36081996 MS.gene40860:intron 50.0%
GATGCAGTGTTCGGCAAAGT+GGG + chr5.2:36081978-36081997 MS.gene40860:intron 50.0%
TAGGCTGAATGCCATGACCT+GGG + chr5.2:36081094-36081113 MS.gene40860:intron 50.0%
TCTCTGCACAAGCTGCTCAA+GGG - chr5.2:36081540-36081559 None:intergenic 50.0%
TGAACGGCAACACTACGCAA+TGG - chr5.2:36081922-36081941 None:intergenic 50.0%
TTAGGCTGAATGCCATGACC+TGG + chr5.2:36081093-36081112 MS.gene40860:intron 50.0%
TTCTCTGCACAAGCTGCTCA+AGG - chr5.2:36081541-36081560 None:intergenic 50.0%
! ATCACCGGTTATCTTGGTGC+TGG + chr5.2:36080622-36080641 MS.gene40860:CDS 50.0%
! TGGAAGTGTGTTCGAACCCT+CGG + chr5.2:36081278-36081297 MS.gene40860:intron 50.0%
!! GTTGGTGTCACTCTCATCAC+CGG + chr5.2:36080607-36080626 MS.gene40860:CDS 50.0%
!! TCTTGGTGCTGGCAAATCCA+CGG + chr5.2:36080633-36080652 MS.gene40860:CDS 50.0%
CACTACGCAATGGACCTGCT+TGG - chr5.2:36081912-36081931 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 36080578 36082183 36080578 ID=MS.gene40860
chr5.2 mRNA 36080578 36082183 36080578 ID=MS.gene40860.t1;Parent=MS.gene40860
chr5.2 exon 36080578 36080654 36080578 ID=MS.gene40860.t1.exon1;Parent=MS.gene40860.t1
chr5.2 CDS 36080578 36080654 36080578 ID=cds.MS.gene40860.t1;Parent=MS.gene40860.t1
chr5.2 exon 36081388 36081567 36081388 ID=MS.gene40860.t1.exon2;Parent=MS.gene40860.t1
chr5.2 CDS 36081388 36081567 36081388 ID=cds.MS.gene40860.t1;Parent=MS.gene40860.t1
chr5.2 exon 36082048 36082183 36082048 ID=MS.gene40860.t1.exon3;Parent=MS.gene40860.t1
chr5.2 CDS 36082048 36082183 36082048 ID=cds.MS.gene40860.t1;Parent=MS.gene40860.t1
Gene Sequence

>MS.gene40860

ATGATGAATCCATTTCTCAACAATCTTCTGTTGGTGTCACTCTCATCACCGGTTATCTTGGTGCTGGCAAATCCACGGTAACCCCATTAATTTTCCCTTTCTATAATTTCTAATTGGGTAGCTTAGTGTTGTGTCTTTTTTCACTTTCATTTTATTTTTGTTATTGGTTCATCGTCATGTACCTATAGCTTGTTGAAATAATAGTGTTTCAATGATTTTTTTTTGTTAAATGAAGATGTTAGTAATGTTTAAATATGATTTTAATATTTTCAGTAGCGTTTTTGGTTTTAGTTCGTGTAAGTATTTTTTATTTGGATTTGGTTTCTGCAAACTCCAAACTCGGTTGCTTTTAATCCTTGATTTCAGCTTATTGGGCATTGGACCGTGTGTCGTGATTATATTGAGTTAAATATGACGTTAAGAAGTAAAAACAACAATTTTTGAATTTGCAGAGACTAAATGTAAACAAAATATTTGTCCATAAGTTCGAGCTCAACTAGAAAATGCCGAAATTGTTAGGCTGAATGCCATGACCTGGGATCCCTAACTTGTATGTGAGTTTTCAAGTGGTTATTGCCACTTCATCTATCATCCAAAAATAAAATCTAAAAAAAAAAAAAAAAAATCTTAGAGGGACTAAATCCAAAAACACTATAATTGTAAGGACTAAAATCATATTTAAACCTTGTAACTGTTATACTGGAAGTGTGTTCGAACCCTCGGCCAATGATTTTGTGAAATGGAATTGTGAATTGGTTGTTTTGAATTTCAGTTAGTGAATCATATATTGAACTCTCAACATGGGAAGAGGATTGCTGTGATATTGAATGAGTTTGGTGAGGAAATTGGAGTGGAAAGAGCATTGATAAATGAAGGGGAAAGTGGTGCAGTTGTTGAAGAATGGGTTGAACTTGCTAATGGCTGTATTTGTTGCACTGTTAAACATAGTTTGGTTCAAGCCCTTGAGCAGCTTGTGCAGAGAAAAGAAAGGTAAAATACAAAACAAACTATTCAAATTTTGAGGCACTAAAAATTTAAACACCGGACTCATAATTACACTTGGGTCGTGCTTCAATAAACATCTTAATTAAGCACGTATAGCATGTACCCTTATCCTATTATTGCTTATGTATATAATATTTTTATATCAAGAGATAAAATAAGTTCAAACTTTTTTCATATAAGCTATAAGCTGGTTTCATAAACTTGCCCGGAGAGCTTATAGAAATAAATTGAAAACAGCTTACAGACATGTCATGAGCTGTTTCCATAAGCTCTCCCTAAGAATCTCACAAGTGCTTATACTAGTGGATAAGCTCAGATAAGCTAATCCAAGCAGGTCCATTGCGTAGTGTTGCCGTTCACTTTTATCAAAGCCCCTTTTGAATCTCTTATAAATAGATGCAGTGTTCGGCAAAGTGGGCCGTATTTTAGTACAATTAAACATTTTTAGGTATTTTGTGTATGTAGGCTAGACCATATATTGCTGGAAACTACTGGTTTGGCTAATCCAGCACCTTTGGCATCTATTCTTTGGTTGGGTGAACAGTTGGAATCTGACGTAAAACTTGATTCTATCGTCACCGTAAGTATCGTTAATGGATGA

Protein sequence

>MS.gene40860.t1

MMNPFLNNLLLVSLSSPVILVLANPRIAVILNEFGEEIGVERALINEGESGAVVEEWVELANGCICCTVKHSLVQALEQLVQRKERLDHILLETTGLANPAPLASILWLGEQLESDVKLDSIVTVSIVNG