AlfalfaGEDB | MS.gene41015

M. sativa cultivar XinJiangDaYe / MS.gene41015

KEGG: -
GO: -
PFAM: PF03018.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41015.t1	XP_003590314.1	90.8	185	17	0	1	185	1	185	3.40E-91	344.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41015.t1	Q9SS03	43.8	185	96	3	8	185	6	189	2.4e-37	156.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41015.t1	G7I8H9	90.8	185	17	0	1	185	1	185	2.4e-91	344.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050380	MS.gene41015	0.836466	9.20E-57	-1.69E-46
MS.gene050381	MS.gene41015	0.803729	3.03E-49	-1.69E-46
MS.gene050809	MS.gene41015	0.819071	1.41E-52	-1.69E-46
MS.gene05240	MS.gene41015	0.804175	2.45E-49	-1.69E-46
MS.gene053406	MS.gene41015	0.825368	4.87E-54	-1.69E-46
MS.gene054290	MS.gene41015	0.846909	1.61E-59	-1.69E-46
MS.gene054742	MS.gene41015	0.822436	2.37E-53	-1.69E-46
MS.gene055124	MS.gene41015	0.813154	2.96E-51	-1.69E-46
MS.gene055959	MS.gene41015	0.817503	3.19E-52	-1.69E-46
MS.gene056590	MS.gene41015	0.811224	7.79E-51	-1.69E-46
MS.gene056591	MS.gene41015	0.810168	1.32E-50	-1.69E-46
MS.gene057544	MS.gene41015	0.810183	1.31E-50	-1.69E-46
MS.gene058093	MS.gene41015	0.819323	1.23E-52	-1.69E-46
MS.gene058158	MS.gene41015	0.806329	8.67E-50	-1.69E-46
MS.gene058177	MS.gene41015	0.804659	1.94E-49	-1.69E-46
MS.gene058179	MS.gene41015	0.810362	1.20E-50	-1.69E-46
MS.gene058180	MS.gene41015	0.804514	2.08E-49	-1.69E-46
MS.gene058218	MS.gene41015	0.830943	2.21E-55	-1.69E-46
MS.gene058840	MS.gene41015	0.810941	8.97E-51	-1.69E-46
MS.gene059037	MS.gene41015	0.814374	1.59E-51	-1.69E-46
MS.gene059038	MS.gene41015	0.808077	3.70E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41015.t1	MTR_1g056370	90.323	186	18	0	1	186	1	186	3.53e-125	350
MS.gene41015.t1	MTR_4g078885	49.375	160	76	3	26	185	30	184	6.89e-49	157
MS.gene41015.t1	MTR_4g013330	48.026	152	75	2	38	186	49	199	3.55e-48	155
MS.gene41015.t1	MTR_3g034030	48.125	160	78	3	26	185	30	184	2.61e-47	153
MS.gene41015.t1	MTR_4g013320	47.368	152	74	3	38	186	51	199	2.86e-47	153
MS.gene41015.t1	MTR_4g013350	47.333	150	77	2	38	186	49	197	4.91e-46	150
MS.gene41015.t1	MTR_4g013325	45.890	146	77	2	42	186	55	199	1.38e-45	149
MS.gene41015.t1	MTR_7g093850	43.750	176	86	4	13	186	30	194	2.42e-45	148
MS.gene41015.t1	MTR_7g093790	40.984	183	105	3	6	186	10	191	2.64e-45	148
MS.gene41015.t1	MTR_4g013345	46.000	150	79	2	38	186	48	196	5.09e-45	147
MS.gene41015.t1	MTR_7g093830	43.750	176	86	4	13	186	29	193	5.14e-45	147
MS.gene41015.t1	MTR_7g093820	42.614	176	88	4	13	186	30	194	6.65e-45	147
MS.gene41015.t1	MTR_1g054525	46.667	150	76	3	38	186	46	192	2.52e-43	143
MS.gene41015.t1	MTR_4g013335	43.624	149	82	2	38	186	50	196	1.87e-42	141
MS.gene41015.t1	MTR_4g013385	44.295	149	82	1	38	186	43	190	3.98e-42	140
MS.gene41015.t1	MTR_0433s0040	44.295	149	82	1	38	186	43	190	3.98e-42	140
MS.gene41015.t1	MTR_7g070390	43.976	166	89	2	21	186	31	192	6.74e-42	139
MS.gene41015.t1	MTR_4g013770	45.578	147	77	3	41	185	45	190	7.49e-41	137
MS.gene41015.t1	MTR_4g073950	36.413	184	112	3	7	186	10	192	1.34e-40	136
MS.gene41015.t1	MTR_4g013310	42.763	152	83	3	36	186	49	197	3.00e-40	135
MS.gene41015.t1	MTR_3g105640	40.642	187	102	3	6	186	5	188	5.07e-40	134
MS.gene41015.t1	MTR_3g105630	41.081	185	105	2	6	186	5	189	6.48e-40	134
MS.gene41015.t1	MTR_7g093870	42.208	154	84	2	38	186	40	193	4.12e-37	128
MS.gene41015.t1	MTR_5g096120	34.197	193	116	4	1	186	1	189	2.30e-35	122
MS.gene41015.t1	MTR_4g013355	38.667	150	80	3	38	186	41	179	2.24e-29	107
MS.gene41015.t1	MTR_4g122110	37.415	147	90	2	41	186	35	180	2.81e-25	96.7
MS.gene41015.t1	MTR_4g122130	35.811	148	91	3	41	186	35	180	5.10e-22	88.2
MS.gene41015.t1	MTR_4g049550	30.612	147	100	2	38	183	30	175	8.87e-20	82.4
MS.gene41015.t1	MTR_4g049570	32.394	142	94	2	43	183	35	175	4.25e-18	77.8
MS.gene41015.t1	MTR_8g099115	33.835	133	77	4	42	165	38	168	3.02e-14	67.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41015.t1	AT1G65870	48.701	154	77	2	33	185	37	189	6.57e-49	157
MS.gene41015.t1	AT3G13650	43.558	163	86	4	24	186	30	186	7.54e-46	149
MS.gene41015.t1	AT5G42500	49.664	149	71	3	38	185	40	185	1.47e-45	149
MS.gene41015.t1	AT5G42510	48.322	149	73	3	38	185	37	182	4.07e-45	147
MS.gene41015.t1	AT1G22900	49.664	149	71	3	38	185	48	193	1.34e-44	146
MS.gene41015.t1	AT1G58170	42.515	167	85	3	30	186	20	185	8.00e-44	144
MS.gene41015.t1	AT1G55210	46.309	149	77	2	38	186	42	187	3.06e-43	142
MS.gene41015.t1	AT1G55210	46.309	149	77	2	38	186	42	187	3.06e-43	142
MS.gene41015.t1	AT2G21100	37.097	186	108	3	1	185	10	187	1.20e-41	139
MS.gene41015.t1	AT2G21100	37.097	186	108	3	1	185	10	187	1.20e-41	139
MS.gene41015.t1	AT5G49040	39.634	164	95	3	24	186	31	191	2.35e-40	135
MS.gene41015.t1	AT3G13662	43.382	136	74	2	38	173	40	172	1.09e-35	123
MS.gene41015.t1	AT2G21110	40.397	151	84	2	41	186	37	186	2.26e-33	117
MS.gene41015.t1	AT3G58090	47.101	138	63	3	53	185	139	271	2.71e-33	119
MS.gene41015.t1	AT5G49030	38.889	162	93	4	7	165	12	170	9.26e-32	120
MS.gene41015.t1	AT3G13660	39.683	126	75	1	61	186	1	125	1.55e-31	111
MS.gene41015.t1	AT4G38700	33.871	186	114	5	10	186	5	190	4.38e-29	106
MS.gene41015.t1	AT4G23690	37.903	124	70	5	42	161	42	162	3.67e-17	75.9
MS.gene41015.t1	AT1G64160	33.898	118	71	4	46	159	41	155	6.91e-15	69.7
MS.gene41015.t1	AT4G11190	33.884	121	73	5	45	161	41	158	6.85e-14	67.0
MS.gene41015.t1	AT4G11210	29.630	162	108	4	4	165	7	162	2.52e-13	65.5

Find 46 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATAAGCTCTGAACTTAAATT+TGG	0.164549	1.3:+41342222	None:intergenic
AAATTTGGTTCTTTAGTTAA+AGG	0.202826	1.3:+41342237	None:intergenic
ATGCTTTGGCTAAGACTATT+TGG	0.206020	1.3:-41342002	MS.gene41015:CDS
TTTCTGAGGGTAAACTTATA+TGG	0.219973	1.3:+41342397	None:intergenic
GAATCATCTCATGGTCCTTT+TGG	0.240305	1.3:-41342282	MS.gene41015:CDS
GGTGAGGCTGCTCCCTTTGT+AGG	0.246807	1.3:+41342110	None:intergenic
AAGAAGCAAGAGGCTGAAAA+TGG	0.251960	1.3:+41342473	None:intergenic
GCTTGGGAGGAATGTTATTA+TGG	0.306263	1.3:-41342085	MS.gene41015:CDS
AATTTAAGTTCAGAGCTTAT+TGG	0.325685	1.3:-41342219	MS.gene41015:CDS
AGCCCAAGGAACCTATTCTT+TGG	0.331068	1.3:-41342193	MS.gene41015:CDS
AATTACTTCATGAGTATTCA+TGG	0.357459	1.3:-41342444	MS.gene41015:CDS
AATTTGGTTCTTTAGTTAAA+GGG	0.362691	1.3:+41342238	None:intergenic
ATTTGGTATAATTCTACATC+TGG	0.367460	1.3:-41341985	MS.gene41015:CDS
TATTTCCATGACATCCTAGA+TGG	0.372994	1.3:-41342345	MS.gene41015:CDS
TTCTGAGGGTAAACTTATAT+GGG	0.380771	1.3:+41342398	None:intergenic
ATTTGCAAGGGGTTATGCTT+TGG	0.411059	1.3:-41342016	MS.gene41015:CDS
ATGCCTATTGTTGGAGGAAC+TGG	0.412156	1.3:-41342048	MS.gene41015:CDS
GGGAGCAGCCTCACCATGCT+TGG	0.438720	1.3:-41342102	MS.gene41015:CDS
GCACCAGTTCCTCCAACAAT+AGG	0.444460	1.3:+41342045	None:intergenic
GTTAGAGAAATGCCTATTGT+TGG	0.446792	1.3:-41342057	MS.gene41015:CDS
AAGCCAAAGAATAGGTTCCT+TGG	0.451718	1.3:+41342190	None:intergenic
AGGACCATGAGATGATTCAT+TGG	0.458423	1.3:+41342287	None:intergenic
CATGGAAATAAAAGTGTAAG+TGG	0.459702	1.3:+41342358	None:intergenic
AGCCAAAGAATAGGTTCCTT+GGG	0.475806	1.3:+41342191	None:intergenic
TTGTTGAGAAGCCAAAGAAT+AGG	0.495361	1.3:+41342182	None:intergenic
AGAAGCAAGAGGCTGAAAAT+GGG	0.514755	1.3:+41342474	None:intergenic
GGAGCAGCCTCACCATGCTT+GGG	0.516739	1.3:-41342101	MS.gene41015:CDS
TTTACAGCTGGAACCTACAA+AGG	0.527260	1.3:-41342123	MS.gene41015:CDS
TTTCACCATCTAGGATGTCA+TGG	0.529125	1.3:+41342340	None:intergenic
GGACCATGAGATGATTCATT+GGG	0.532395	1.3:+41342288	None:intergenic
AGAGAAATGCCTATTGTTGG+AGG	0.533145	1.3:-41342054	MS.gene41015:CDS
TTACAGCTGGAACCTACAAA+GGG	0.548749	1.3:-41342122	MS.gene41015:CDS
CTGGTGCTTTCAGATTTGCA+AGG	0.550900	1.3:-41342029	MS.gene41015:CDS
AGTATGTAAGTGGCTCCAAA+AGG	0.553653	1.3:+41342267	None:intergenic
TGGTGCTTTCAGATTTGCAA+GGG	0.553765	1.3:-41342028	MS.gene41015:CDS
CCATGAGATGATTCATTGGG+TGG	0.558415	1.3:+41342291	None:intergenic
AGGGTTGTCTAGTATGTAAG+TGG	0.563851	1.3:+41342257	None:intergenic
TATTCTTTGGCTTCTCAACA+AGG	0.573141	1.3:-41342180	MS.gene41015:CDS
ATGGAAATAAAAGTGTAAGT+GGG	0.573723	1.3:+41342359	None:intergenic
GAGCTTATTGGAAGAGCCCA+AGG	0.578477	1.3:-41342207	MS.gene41015:CDS
GCAGCCTCACCATGCTTGGG+AGG	0.595405	1.3:-41342098	MS.gene41015:CDS
AATAACATTCCTCCCAAGCA+TGG	0.596205	1.3:+41342089	None:intergenic
CCACCCAATGAATCATCTCA+TGG	0.605763	1.3:-41342291	MS.gene41015:CDS
AATTAATTAAAAGAAGCAAG+AGG	0.637623	1.3:+41342463	None:intergenic
CATTCCTCCCAAGCATGGTG+AGG	0.641930	1.3:+41342094	None:intergenic
GGTGCTTTCAGATTTGCAAG+GGG	0.678373	1.3:-41342027	MS.gene41015:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTAATTAAAAGAAGCAAG+AGG	+	chr1.3:41341962-41341981	None:intergenic	20.0%
!!!	AAATTTGGTTCTTTAGTTAA+AGG	+	chr1.3:41342188-41342207	None:intergenic	20.0%
!!!	AATTTGGTTCTTTAGTTAAA+GGG	+	chr1.3:41342187-41342206	None:intergenic	20.0%
!!!	ATTAATTTTGTTTTTACAGC+TGG	-	chr1.3:41342287-41342306	MS.gene41015:CDS	20.0%
!	AATTACTTCATGAGTATTCA+TGG	-	chr1.3:41341978-41341997	MS.gene41015:CDS	25.0%
!	ATTTGGTATAATTCTACATC+TGG	-	chr1.3:41342437-41342456	MS.gene41015:CDS	25.0%
!!	AAAACTTTGTAGATTTTCTG+AGG	+	chr1.3:41342042-41342061	None:intergenic	25.0%
!!	AAACTTTGTAGATTTTCTGA+GGG	+	chr1.3:41342041-41342060	None:intergenic	25.0%
!!	AATTTAAGTTCAGAGCTTAT+TGG	-	chr1.3:41342203-41342222	MS.gene41015:CDS	25.0%
!!	ATGGAAATAAAAGTGTAAGT+GGG	+	chr1.3:41342066-41342085	None:intergenic	25.0%
!!!	ATAAGCTCTGAACTTAAATT+TGG	+	chr1.3:41342203-41342222	None:intergenic	25.0%
!!!	TTGATAATTTTGAGTGTAGT+TGG	+	chr1.3:41342110-41342129	None:intergenic	25.0%
!	CATGGAAATAAAAGTGTAAG+TGG	+	chr1.3:41342067-41342086	None:intergenic	30.0%
!	TTCTGAGGGTAAACTTATAT+GGG	+	chr1.3:41342027-41342046	None:intergenic	30.0%
!	TTTCTGAGGGTAAACTTATA+TGG	+	chr1.3:41342028-41342047	None:intergenic	30.0%
	ATGCTTTGGCTAAGACTATT+TGG	-	chr1.3:41342420-41342439	MS.gene41015:CDS	35.0%
	GTTAGAGAAATGCCTATTGT+TGG	-	chr1.3:41342365-41342384	MS.gene41015:CDS	35.0%
	TATTTCCATGACATCCTAGA+TGG	-	chr1.3:41342077-41342096	MS.gene41015:CDS	35.0%
	TTGTTGAGAAGCCAAAGAAT+AGG	+	chr1.3:41342243-41342262	None:intergenic	35.0%
!	TATTCTTTGGCTTCTCAACA+AGG	-	chr1.3:41342242-41342261	MS.gene41015:CDS	35.0%
!!!	TAGTTGGTTTTTCACCATCT+AGG	+	chr1.3:41342094-41342113	None:intergenic	35.0%
	AAGAAGCAAGAGGCTGAAAA+TGG	+	chr1.3:41341952-41341971	None:intergenic	40.0%
	AAGCCAAAGAATAGGTTCCT+TGG	+	chr1.3:41342235-41342254	None:intergenic	40.0%
	AATAACATTCCTCCCAAGCA+TGG	+	chr1.3:41342336-41342355	None:intergenic	40.0%
	AGAAGCAAGAGGCTGAAAAT+GGG	+	chr1.3:41341951-41341970	None:intergenic	40.0%
	AGAGAAATGCCTATTGTTGG+AGG	-	chr1.3:41342368-41342387	MS.gene41015:CDS	40.0%
	AGCCAAAGAATAGGTTCCTT+GGG	+	chr1.3:41342234-41342253	None:intergenic	40.0%
	AGGACCATGAGATGATTCAT+TGG	+	chr1.3:41342138-41342157	None:intergenic	40.0%
	AGGGTTGTCTAGTATGTAAG+TGG	+	chr1.3:41342168-41342187	None:intergenic	40.0%
	AGTATGTAAGTGGCTCCAAA+AGG	+	chr1.3:41342158-41342177	None:intergenic	40.0%
	ATTTGCAAGGGGTTATGCTT+TGG	-	chr1.3:41342406-41342425	MS.gene41015:CDS	40.0%
	GCTTGGGAGGAATGTTATTA+TGG	-	chr1.3:41342337-41342356	MS.gene41015:CDS	40.0%
	GGACCATGAGATGATTCATT+GGG	+	chr1.3:41342137-41342156	None:intergenic	40.0%
	TGGTGCTTTCAGATTTGCAA+GGG	-	chr1.3:41342394-41342413	MS.gene41015:CDS	40.0%
	TTACAGCTGGAACCTACAAA+GGG	-	chr1.3:41342300-41342319	MS.gene41015:CDS	40.0%
	TTTACAGCTGGAACCTACAA+AGG	-	chr1.3:41342299-41342318	MS.gene41015:CDS	40.0%
!	GAATCATCTCATGGTCCTTT+TGG	-	chr1.3:41342140-41342159	MS.gene41015:CDS	40.0%
!	TTTCACCATCTAGGATGTCA+TGG	+	chr1.3:41342085-41342104	None:intergenic	40.0%
	AGCCCAAGGAACCTATTCTT+TGG	-	chr1.3:41342229-41342248	MS.gene41015:CDS	45.0%
	ATGCCTATTGTTGGAGGAAC+TGG	-	chr1.3:41342374-41342393	MS.gene41015:CDS	45.0%
	CCACCCAATGAATCATCTCA+TGG	-	chr1.3:41342131-41342150	MS.gene41015:CDS	45.0%
	CCATGAGATGATTCATTGGG+TGG	+	chr1.3:41342134-41342153	None:intergenic	45.0%
	CTGGTGCTTTCAGATTTGCA+AGG	-	chr1.3:41342393-41342412	MS.gene41015:CDS	45.0%
	GGTGCTTTCAGATTTGCAAG+GGG	-	chr1.3:41342395-41342414	MS.gene41015:CDS	45.0%
	GAGCTTATTGGAAGAGCCCA+AGG	-	chr1.3:41342215-41342234	MS.gene41015:CDS	50.0%
	GCACCAGTTCCTCCAACAAT+AGG	+	chr1.3:41342380-41342399	None:intergenic	50.0%
	CATTCCTCCCAAGCATGGTG+AGG	+	chr1.3:41342331-41342350	None:intergenic	55.0%
	GGAGCAGCCTCACCATGCTT+GGG	-	chr1.3:41342321-41342340	MS.gene41015:CDS	60.0%
	GGTGAGGCTGCTCCCTTTGT+AGG	+	chr1.3:41342315-41342334	None:intergenic	60.0%
	GCAGCCTCACCATGCTTGGG+AGG	-	chr1.3:41342324-41342343	MS.gene41015:CDS	65.0%
	GGGAGCAGCCTCACCATGCT+TGG	-	chr1.3:41342320-41342339	MS.gene41015:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	41341942	41342502	41341942	ID=MS.gene41015
chr1.3	mRNA	41341942	41342502	41341942	ID=MS.gene41015.t1;Parent=MS.gene41015
chr1.3	exon	41341942	41342502	41341942	ID=MS.gene41015.t1.exon1;Parent=MS.gene41015.t1
chr1.3	CDS	41341942	41342502	41341942	ID=cds.MS.gene41015.t1;Parent=MS.gene41015.t1

Gene Sequence

>MS.gene41015

ATGGTTCCCATTTTCAGCCTCTTGCTTCTTTTAATTAATTACTTCATGAGTATTCATGGAAGAGTGATGTCTTTAGAAGAATCCCATATAAGTTTACCCTCAGAAAATCTACAAAGTTTTACCCACTTACACTTTTATTTCCATGACATCCTAGATGGTGAAAAACCAACTACACTCAAAATTATCAATCCACCCAATGAATCATCTCATGGTCCTTTTGGAGCCACTTACATACTAGACAACCCTTTAACTAAAGAACCAAATTTAAGTTCAGAGCTTATTGGAAGAGCCCAAGGAACCTATTCTTTGGCTTCTCAACAAGGTGATTTTGCATTTAAGATCGATATTAATTTTGTTTTTACAGCTGGAACCTACAAAGGGAGCAGCCTCACCATGCTTGGGAGGAATGTTATTATGGATGAAGTTAGAGAAATGCCTATTGTTGGAGGAACTGGTGCTTTCAGATTTGCAAGGGGTTATGCTTTGGCTAAGACTATTTGGTATAATTCTACATCTGGAAATGCTATTGAAGAGTTTGATATTATAATTTTACATTTCTAA

Protein sequence

>MS.gene41015.t1

MVPIFSLLLLLINYFMSIHGRVMSLEESHISLPSENLQSFTHLHFYFHDILDGEKPTTLKIINPPNESSHGPFGATYILDNPLTKEPNLSSELIGRAQGTYSLASQQGDFAFKIDINFVFTAGTYKGSSLTMLGRNVIMDEVREMPIVGGTGAFRFARGYALAKTIWYNSTSGNAIEEFDIIILHF