Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059038.t1 | XP_003594829.1 | 98.1 | 157 | 3 | 0 | 1 | 157 | 1 | 157 | 1.00E-80 | 309.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059038.t1 | P93333 | 98.1 | 157 | 3 | 0 | 1 | 157 | 1 | 157 | 2.5e-83 | 309.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059038.t1 | A0A396J749 | 98.1 | 157 | 3 | 0 | 1 | 157 | 1 | 157 | 7.4e-81 | 309.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049634 | MS.gene059038 | 0.800424 | 1.45E-48 | -1.69E-46 |
| MS.gene050380 | MS.gene059038 | 0.821642 | 3.62E-53 | -1.69E-46 |
| MS.gene050381 | MS.gene059038 | 0.812794 | 3.54E-51 | -1.69E-46 |
| MS.gene052805 | MS.gene059038 | 0.80138 | 9.25E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene059038 | 0.828181 | 1.04E-54 | -1.69E-46 |
| MS.gene055124 | MS.gene059038 | 0.837064 | 6.48E-57 | -1.69E-46 |
| MS.gene055154 | MS.gene059038 | 0.813375 | 2.64E-51 | -1.69E-46 |
| MS.gene055202 | MS.gene059038 | 0.811791 | 5.87E-51 | -1.69E-46 |
| MS.gene055959 | MS.gene059038 | 0.858386 | 8.54E-63 | -1.69E-46 |
| MS.gene058093 | MS.gene059038 | 0.878575 | 2.51E-69 | -1.69E-46 |
| MS.gene058099 | MS.gene059038 | 0.859846 | 3.12E-63 | -1.69E-46 |
| MS.gene058108 | MS.gene059038 | 0.81801 | 2.45E-52 | -1.69E-46 |
| MS.gene058109 | MS.gene059038 | 0.834125 | 3.59E-56 | -1.69E-46 |
| MS.gene058171 | MS.gene059038 | 0.830981 | 2.16E-55 | -1.69E-46 |
| MS.gene058173 | MS.gene059038 | 0.829845 | 4.10E-55 | -1.69E-46 |
| MS.gene058175 | MS.gene059038 | 0.83412 | 3.60E-56 | -1.69E-46 |
| MS.gene058176 | MS.gene059038 | 0.819687 | 1.02E-52 | -1.69E-46 |
| MS.gene058179 | MS.gene059038 | 0.875531 | 2.86E-68 | -1.69E-46 |
| MS.gene058180 | MS.gene059038 | 0.849069 | 4.10E-60 | -1.69E-46 |
| MS.gene058840 | MS.gene059038 | 0.810928 | 9.03E-51 | -1.69E-46 |
| MS.gene059037 | MS.gene059038 | 0.92903 | 1.25E-92 | -1.69E-46 |
| MS.gene059038 | MS.gene059044 | 0.826864 | 2.15E-54 | -1.69E-46 |
| MS.gene059038 | MS.gene059045 | 0.852199 | 5.40E-61 | -1.69E-46 |
| MS.gene059038 | MS.gene059402 | 0.801139 | 1.04E-48 | -1.69E-46 |
| MS.gene059038 | MS.gene061402 | -0.807729 | 4.38E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene061565 | 0.805368 | 1.38E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene062071 | 0.818466 | 1.93E-52 | -1.69E-46 |
| MS.gene059038 | MS.gene063073 | 0.83813 | 3.45E-57 | -1.69E-46 |
| MS.gene059038 | MS.gene065240 | 0.803496 | 3.39E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene068296 | 0.849233 | 3.69E-60 | -1.69E-46 |
| MS.gene059038 | MS.gene068297 | 0.882787 | 7.77E-71 | -1.69E-46 |
| MS.gene059038 | MS.gene068317 | 0.80028 | 1.55E-48 | -1.69E-46 |
| MS.gene059038 | MS.gene068917 | 0.817701 | 2.88E-52 | -1.69E-46 |
| MS.gene059038 | MS.gene069874 | 0.800401 | 1.47E-48 | -1.69E-46 |
| MS.gene059038 | MS.gene071838 | 0.850637 | 1.49E-60 | -1.69E-46 |
| MS.gene059038 | MS.gene071839 | 0.809487 | 1.85E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene071843 | 0.830315 | 3.15E-55 | -1.69E-46 |
| MS.gene059038 | MS.gene071942 | 0.847569 | 1.06E-59 | -1.69E-46 |
| MS.gene059038 | MS.gene072378 | 0.824571 | 7.51E-54 | -1.69E-46 |
| MS.gene059038 | MS.gene072806 | 0.816063 | 6.71E-52 | -1.69E-46 |
| MS.gene059038 | MS.gene073183 | 0.806622 | 7.52E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene073526 | 0.804076 | 2.57E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene07427 | -0.800182 | 1.63E-48 | -1.69E-46 |
| MS.gene059038 | MS.gene07591 | 0.836457 | 9.25E-57 | -1.69E-46 |
| MS.gene059038 | MS.gene08407 | 0.825948 | 3.55E-54 | -1.69E-46 |
| MS.gene059038 | MS.gene21350 | 0.801077 | 1.07E-48 | -1.69E-46 |
| MS.gene059038 | MS.gene21577 | 0.819091 | 1.39E-52 | -1.69E-46 |
| MS.gene059038 | MS.gene21757 | 0.844993 | 5.36E-59 | -1.69E-46 |
| MS.gene059038 | MS.gene21758 | 0.857137 | 2.00E-62 | -1.69E-46 |
| MS.gene059038 | MS.gene21768 | 0.84873 | 5.09E-60 | -1.69E-46 |
| MS.gene059038 | MS.gene21772 | 0.87768 | 5.18E-69 | -1.69E-46 |
| MS.gene059038 | MS.gene21851 | 0.852332 | 4.95E-61 | -1.69E-46 |
| MS.gene059038 | MS.gene21856 | 0.849397 | 3.32E-60 | -1.69E-46 |
| MS.gene059038 | MS.gene21867 | 0.850825 | 1.32E-60 | -1.69E-46 |
| MS.gene059038 | MS.gene21868 | 0.835836 | 1.33E-56 | -1.69E-46 |
| MS.gene059038 | MS.gene23573 | 0.804008 | 2.66E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene24974 | 0.888609 | 5.07E-73 | -1.69E-46 |
| MS.gene059038 | MS.gene26243 | 0.820805 | 5.65E-53 | -1.69E-46 |
| MS.gene059038 | MS.gene26403 | 0.861574 | 9.32E-64 | -1.69E-46 |
| MS.gene059038 | MS.gene26517 | 0.824972 | 6.04E-54 | -1.69E-46 |
| MS.gene059038 | MS.gene27550 | 0.818661 | 1.75E-52 | -1.69E-46 |
| MS.gene059038 | MS.gene27779 | 0.837175 | 6.07E-57 | -1.69E-46 |
| MS.gene059038 | MS.gene27827 | 0.814546 | 1.46E-51 | -1.69E-46 |
| MS.gene059038 | MS.gene28444 | -0.806865 | 6.68E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene33428 | 0.876514 | 1.31E-68 | -1.69E-46 |
| MS.gene059038 | MS.gene33682 | 0.87938 | 1.31E-69 | -1.69E-46 |
| MS.gene059038 | MS.gene35208 | 0.825039 | 5.83E-54 | -1.69E-46 |
| MS.gene059038 | MS.gene37192 | 0.809409 | 1.92E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene38094 | 0.810589 | 1.07E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene38095 | 0.826781 | 2.25E-54 | -1.69E-46 |
| MS.gene059038 | MS.gene38097 | 0.806779 | 6.97E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene38155 | 0.824254 | 8.92E-54 | -1.69E-46 |
| MS.gene059038 | MS.gene38172 | 0.802578 | 5.25E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene38173 | 0.807412 | 5.12E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene38435 | 0.801785 | 7.64E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene38706 | 0.810969 | 8.85E-51 | -1.69E-46 |
| MS.gene059038 | MS.gene39533 | 0.813732 | 2.21E-51 | -1.69E-46 |
| MS.gene059038 | MS.gene39535 | 0.83828 | 3.16E-57 | -1.69E-46 |
| MS.gene059038 | MS.gene40171 | 0.802017 | 6.85E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene41015 | 0.808077 | 3.70E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene44712 | 0.805267 | 1.45E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene44722 | 0.800857 | 1.18E-48 | -1.69E-46 |
| MS.gene059038 | MS.gene45228 | 0.805776 | 1.13E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene46024 | 0.837159 | 6.13E-57 | -1.69E-46 |
| MS.gene059038 | MS.gene46025 | 0.853723 | 1.98E-61 | -1.69E-46 |
| MS.gene059038 | MS.gene46027 | 0.834647 | 2.66E-56 | -1.69E-46 |
| MS.gene059038 | MS.gene46057 | 0.817715 | 2.86E-52 | -1.69E-46 |
| MS.gene059038 | MS.gene46106 | 0.800178 | 1.63E-48 | -1.69E-46 |
| MS.gene059038 | MS.gene46434 | 0.815651 | 8.29E-52 | -1.69E-46 |
| MS.gene059038 | MS.gene46779 | 0.819747 | 9.87E-53 | -1.69E-46 |
| MS.gene059038 | MS.gene47576 | 0.848306 | 6.67E-60 | -1.69E-46 |
| MS.gene059038 | MS.gene48183 | 0.822002 | 2.99E-53 | -1.69E-46 |
| MS.gene059038 | MS.gene48314 | 0.818003 | 2.46E-52 | -1.69E-46 |
| MS.gene059038 | MS.gene48807 | 0.800698 | 1.28E-48 | -1.69E-46 |
| MS.gene059038 | MS.gene51523 | 0.820447 | 6.83E-53 | -1.69E-46 |
| MS.gene059038 | MS.gene51620 | 0.819186 | 1.33E-52 | -1.69E-46 |
| MS.gene059038 | MS.gene52700 | 0.862861 | 3.75E-64 | -1.69E-46 |
| MS.gene059038 | MS.gene56509 | 0.800673 | 1.29E-48 | -1.69E-46 |
| MS.gene059038 | MS.gene57807 | 0.808974 | 2.38E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene58124 | -0.816916 | 4.32E-52 | -1.69E-46 |
| MS.gene059038 | MS.gene59001 | 0.833726 | 4.52E-56 | -1.69E-46 |
| MS.gene059038 | MS.gene59005 | 0.876732 | 1.11E-68 | -1.69E-46 |
| MS.gene059038 | MS.gene59006 | 0.856979 | 2.23E-62 | -1.69E-46 |
| MS.gene059038 | MS.gene59394 | 0.885541 | 7.44E-72 | -1.69E-46 |
| MS.gene059038 | MS.gene59395 | 0.859796 | 3.23E-63 | -1.69E-46 |
| MS.gene059038 | MS.gene59396 | 0.882533 | 9.62E-71 | -1.69E-46 |
| MS.gene059038 | MS.gene60477 | 0.858192 | 9.75E-63 | -1.69E-46 |
| MS.gene059038 | MS.gene60649 | 0.804346 | 2.26E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene61194 | 0.884378 | 2.02E-71 | -1.69E-46 |
| MS.gene059038 | MS.gene62114 | 0.884273 | 2.21E-71 | -1.69E-46 |
| MS.gene059038 | MS.gene62120 | 0.843817 | 1.11E-58 | -1.69E-46 |
| MS.gene059038 | MS.gene63691 | 0.802068 | 6.69E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene63731 | 0.808059 | 3.73E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene64372 | 0.80543 | 1.34E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene64686 | 0.852443 | 4.60E-61 | -1.69E-46 |
| MS.gene059038 | MS.gene64687 | 0.865817 | 4.48E-65 | -1.69E-46 |
| MS.gene059038 | MS.gene65724 | 0.801045 | 1.08E-48 | -1.69E-46 |
| MS.gene059038 | MS.gene68121 | 0.80384 | 2.88E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene68722 | 0.801124 | 1.04E-48 | -1.69E-46 |
| MS.gene059038 | MS.gene70420 | 0.809353 | 1.97E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene71150 | 0.808012 | 3.82E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene71525 | 0.80416 | 2.47E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene73913 | 0.800234 | 1.59E-48 | -1.69E-46 |
| MS.gene059038 | MS.gene75392 | 0.85114 | 1.08E-60 | -1.69E-46 |
| MS.gene059038 | MS.gene75396 | 0.878875 | 1.97E-69 | -1.69E-46 |
| MS.gene059038 | MS.gene76539 | 0.805661 | 1.20E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene77223 | 0.829145 | 6.06E-55 | -1.69E-46 |
| MS.gene059038 | MS.gene77471 | 0.824935 | 6.16E-54 | -1.69E-46 |
| MS.gene059038 | MS.gene78687 | 0.833837 | 4.24E-56 | -1.69E-46 |
| MS.gene059038 | MS.gene79443 | 0.807457 | 5.01E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene79445 | 0.802592 | 5.21E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene82244 | 0.805093 | 1.58E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene84374 | 0.821703 | 3.51E-53 | -1.69E-46 |
| MS.gene059038 | MS.gene84568 | 0.829177 | 5.95E-55 | -1.69E-46 |
| MS.gene059038 | MS.gene85154 | 0.800732 | 1.26E-48 | -1.69E-46 |
| MS.gene059038 | MS.gene85298 | 0.814802 | 1.28E-51 | -1.69E-46 |
| MS.gene059038 | MS.gene85638 | 0.810195 | 1.30E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene86720 | 0.809482 | 1.85E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene89261 | 0.816183 | 6.31E-52 | -1.69E-46 |
| MS.gene059038 | MS.gene90479 | 0.806018 | 1.01E-49 | -1.69E-46 |
| MS.gene059038 | MS.gene91973 | 0.813243 | 2.83E-51 | -1.69E-46 |
| MS.gene059038 | MS.gene92808 | 0.819251 | 1.28E-52 | -1.69E-46 |
| MS.gene059038 | MS.gene94790 | 0.860922 | 1.47E-63 | -1.69E-46 |
| MS.gene059038 | MS.gene96173 | 0.844307 | 8.20E-59 | -1.69E-46 |
| MS.gene059038 | MS.gene96584 | 0.801114 | 1.05E-48 | -1.69E-46 |
| MS.gene059038 | MS.gene97499 | 0.87783 | 4.59E-69 | -1.69E-46 |
| MS.gene059038 | MS.gene97500 | 0.875388 | 3.21E-68 | -1.69E-46 |
| MS.gene059038 | MS.gene97682 | 0.800403 | 1.47E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059038.t1 | MTR_2g035100 | 98.089 | 157 | 3 | 0 | 1 | 157 | 1 | 157 | 7.40e-108 | 304 |
| MS.gene059038.t1 | MTR_2g035105 | 96.815 | 157 | 5 | 0 | 1 | 157 | 1 | 157 | 1.55e-106 | 301 |
| MS.gene059038.t1 | MTR_2g035130 | 85.987 | 157 | 22 | 0 | 1 | 157 | 1 | 157 | 3.42e-94 | 270 |
| MS.gene059038.t1 | MTR_2g035150 | 83.439 | 157 | 26 | 0 | 1 | 157 | 1 | 157 | 7.24e-92 | 264 |
| MS.gene059038.t1 | MTR_2g035120 | 82.166 | 157 | 28 | 0 | 1 | 157 | 1 | 157 | 8.63e-92 | 263 |
| MS.gene059038.t1 | MTR_6g033450 | 72.611 | 157 | 43 | 0 | 1 | 157 | 1 | 157 | 1.74e-79 | 232 |
| MS.gene059038.t1 | MTR_4g120970 | 72.611 | 157 | 43 | 0 | 1 | 157 | 1 | 157 | 1.74e-79 | 232 |
| MS.gene059038.t1 | MTR_2g035320 | 63.694 | 157 | 57 | 0 | 1 | 157 | 1 | 157 | 2.63e-67 | 201 |
| MS.gene059038.t1 | MTR_2g035190 | 60.510 | 157 | 62 | 0 | 1 | 157 | 1 | 157 | 1.07e-64 | 195 |
| MS.gene059038.t1 | MTR_2g035210 | 59.873 | 157 | 63 | 0 | 1 | 157 | 1 | 157 | 1.47e-63 | 192 |
| MS.gene059038.t1 | MTR_2g035220 | 60.510 | 157 | 62 | 0 | 1 | 157 | 1 | 157 | 1.68e-62 | 189 |
| MS.gene059038.t1 | MTR_2g035170 | 60.510 | 157 | 62 | 0 | 1 | 157 | 1 | 157 | 7.96e-62 | 187 |
| MS.gene059038.t1 | MTR_2g435310 | 40.385 | 156 | 92 | 1 | 1 | 155 | 1 | 156 | 9.08e-39 | 129 |
| MS.gene059038.t1 | MTR_4g120760 | 39.744 | 156 | 93 | 1 | 1 | 155 | 1 | 156 | 1.79e-36 | 123 |
| MS.gene059038.t1 | MTR_4g120760 | 39.744 | 156 | 93 | 1 | 1 | 155 | 1 | 156 | 1.94e-36 | 123 |
| MS.gene059038.t1 | MTR_2g035320 | 61.458 | 96 | 37 | 0 | 62 | 157 | 27 | 122 | 2.58e-35 | 119 |
| MS.gene059038.t1 | MTR_4g120950 | 44.720 | 161 | 82 | 5 | 1 | 157 | 1 | 158 | 4.70e-33 | 115 |
| MS.gene059038.t1 | MTR_4g120940 | 61.290 | 93 | 33 | 1 | 65 | 157 | 30 | 119 | 5.65e-30 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCAACAACCTTTGGAATA+AGG | 0.213257 | 2.4:+56067854 | None:intergenic |
| AACGAAAGTAAGTTTCTTGA+TGG | 0.287513 | 2.4:+56067786 | None:intergenic |
| CCCTTGATTTCCTCTTCACT+TGG | 0.329042 | 2.4:+56066985 | None:intergenic |
| CTTCAACAATTTCAATACTT+TGG | 0.342783 | 2.4:+56067827 | None:intergenic |
| GAGATCTTCTCCACTGTGTC+TGG | 0.364866 | 2.4:+56067093 | None:intergenic |
| AAGAAACTTACTTTCGTTGA+AGG | 0.370871 | 2.4:-56067781 | MS.gene059038:intron |
| GGCTCTTGAAGGTTACGTTT+TGG | 0.372797 | 2.4:-56066932 | MS.gene059038:CDS |
| AGTAAGTTTCTTGATGGTTC+CGG | 0.383147 | 2.4:+56067792 | None:intergenic |
| TGGATGGCATCAACAACCTT+TGG | 0.398526 | 2.4:+56067847 | None:intergenic |
| CAAAGTATTGAAATTGTTGA+AGG | 0.421501 | 2.4:-56067826 | MS.gene059038:CDS |
| GCTTACAACTACAGCATAGT+TGG | 0.425478 | 2.4:-56067129 | MS.gene059038:CDS |
| AACAATTTCAATACTTTGGA+TGG | 0.439896 | 2.4:+56067831 | None:intergenic |
| ATTGAAATTGTTGAAGGAAA+CGG | 0.445615 | 2.4:-56067820 | MS.gene059038:CDS |
| GTTATCAGAATCTGTAACTA+GGG | 0.446856 | 2.4:+56067876 | None:intergenic |
| AAATCAAGGGTGGCAAAGCT+AGG | 0.448601 | 2.4:-56066972 | MS.gene059038:CDS |
| TTTGCAATGGATCCTCCATC+TGG | 0.453770 | 2.4:+56067045 | None:intergenic |
| TTTGAGTCTAAATTATCTCC+AGG | 0.474273 | 2.4:-56067069 | MS.gene059038:CDS |
| CATGGGTGTATTCAACTTTG+AGG | 0.475033 | 2.4:-56067941 | MS.gene059038:CDS |
| GGTGGCAAAGCTAGGGGTGA+TGG | 0.489081 | 2.4:-56066964 | MS.gene059038:CDS |
| AATCAAGGGTGGCAAAGCTA+GGG | 0.491277 | 2.4:-56066971 | MS.gene059038:CDS |
| AGCTATATGCTTTAGTAACC+AGG | 0.495721 | 2.4:+56066904 | None:intergenic |
| ACTGTGAAATACTTCACAAA+AGG | 0.498744 | 2.4:-56067018 | MS.gene059038:CDS |
| ACCAAGTGAAGAGGAAATCA+AGG | 0.498836 | 2.4:-56066986 | MS.gene059038:CDS |
| ATGGATCCTCCATCTGGCCC+TGG | 0.500716 | 2.4:+56067051 | None:intergenic |
| TACAGCATAGTTGGAGGTGG+TGG | 0.504513 | 2.4:-56067120 | MS.gene059038:CDS |
| GGTTATCAGAATCTGTAACT+AGG | 0.504628 | 2.4:+56067875 | None:intergenic |
| TTCTGATAACCTTATTCCAA+AGG | 0.526563 | 2.4:-56067863 | MS.gene059038:CDS |
| AAAGTATGATTTGCACAAAG+TGG | 0.527363 | 2.4:-56067175 | MS.gene059038:CDS |
| TTTCACAGTAAGCTTTGCAA+TGG | 0.527944 | 2.4:+56067032 | None:intergenic |
| AGGGCTTTGTAAAGTCTAGC+AGG | 0.529123 | 2.4:+56067895 | None:intergenic |
| TGGAGATAATTTAGACTCAA+AGG | 0.536882 | 2.4:+56067071 | None:intergenic |
| GTTGAAGGAAACGGTGGCGC+CGG | 0.542615 | 2.4:-56067811 | MS.gene059038:CDS |
| AAATTATCTCCAGGGCCAGA+TGG | 0.556459 | 2.4:-56067060 | MS.gene059038:CDS |
| TACAACTACAGCATAGTTGG+AGG | 0.596062 | 2.4:-56067126 | MS.gene059038:CDS |
| TGATGCTGCACCAAGTGAAG+AGG | 0.603594 | 2.4:-56066995 | MS.gene059038:CDS |
| TTGAGTCTAAATTATCTCCA+GGG | 0.612549 | 2.4:-56067068 | MS.gene059038:CDS |
| AACTACAGCATAGTTGGAGG+TGG | 0.623337 | 2.4:-56067123 | MS.gene059038:CDS |
| TTATCTCCAGGGCCAGATGG+AGG | 0.631574 | 2.4:-56067057 | MS.gene059038:CDS |
| ATCAAGGGTGGCAAAGCTAG+GGG | 0.634094 | 2.4:-56066970 | MS.gene059038:CDS |
| AGCAGGAGCTACAATAGATG+TGG | 0.641681 | 2.4:+56067912 | None:intergenic |
| CCAAGTGAAGAGGAAATCAA+GGG | 0.664848 | 2.4:-56066985 | MS.gene059038:CDS |
| GAAATTGTTGAAGGAAACGG+TGG | 0.666040 | 2.4:-56067817 | MS.gene059038:CDS |
| AGTGAAGAGGAAATCAAGGG+TGG | 0.695634 | 2.4:-56066982 | MS.gene059038:CDS |
| TGGTGGACTTCCAGACACAG+TGG | 0.728449 | 2.4:-56067103 | MS.gene059038:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATATAATAAAAGAAAAG+AGG | + | chr2.4:56067488-56067507 | None:intergenic | 10.0% |
| !!! | TCTTTTCTTTTATTATATAT+TGG | - | chr2.4:56067487-56067506 | MS.gene059038:intron | 10.0% |
| !!! | CTTTTAAAAAATAGTTTTTC+TGG | + | chr2.4:56067422-56067441 | None:intergenic | 15.0% |
| !!! | GTGTTATTATATACATTTAA+TGG | - | chr2.4:56067353-56067372 | MS.gene059038:intron | 15.0% |
| !! | AAGATACATAACATAAATTG+TGG | - | chr2.4:56067250-56067269 | MS.gene059038:intron | 20.0% |
| !! | ATATATGTGAAAAATTGAAG+TGG | - | chr2.4:56067104-56067123 | MS.gene059038:CDS | 20.0% |
| ! | AACAAGAATCAAACATTTGA+TGG | + | chr2.4:56067448-56067467 | None:intergenic | 25.0% |
| ! | AACAATTTCAATACTTTGGA+TGG | + | chr2.4:56067027-56067046 | None:intergenic | 25.0% |
| ! | ATACATAACATAAATTGTGG+AGG | - | chr2.4:56067253-56067272 | MS.gene059038:intron | 25.0% |
| ! | ATTGAAATTGTTGAAGGAAA+CGG | - | chr2.4:56067035-56067054 | MS.gene059038:CDS | 25.0% |
| ! | CTTCAACAATTTCAATACTT+TGG | + | chr2.4:56067031-56067050 | None:intergenic | 25.0% |
| !!! | AATTAACCGTCTTTATTTTG+AGG | + | chr2.4:56067193-56067212 | None:intergenic | 25.0% |
| !!! | CAAAGTATTGAAATTGTTGA+AGG | - | chr2.4:56067029-56067048 | MS.gene059038:CDS | 25.0% |
| AACGAAAGTAAGTTTCTTGA+TGG | + | chr2.4:56067072-56067091 | None:intergenic | 30.0% | |
| AAGAAACTTACTTTCGTTGA+AGG | - | chr2.4:56067074-56067093 | MS.gene059038:CDS | 30.0% | |
| ACTGTGAAATACTTCACAAA+AGG | - | chr2.4:56067837-56067856 | MS.gene059038:CDS | 30.0% | |
| CCATATCCTCAAAATAAAGA+CGG | - | chr2.4:56067184-56067203 | MS.gene059038:CDS | 30.0% | |
| GTTATCAGAATCTGTAACTA+GGG | + | chr2.4:56066982-56067001 | None:intergenic | 30.0% | |
| TGGAGATAATTTAGACTCAA+AGG | + | chr2.4:56067787-56067806 | None:intergenic | 30.0% | |
| TTCATGCAGTGAAAATTGTT+TGG | + | chr2.4:56067162-56067181 | None:intergenic | 30.0% | |
| TTCTGATAACCTTATTCCAA+AGG | - | chr2.4:56066992-56067011 | MS.gene059038:CDS | 30.0% | |
| TTGAGTCTAAATTATCTCCA+GGG | - | chr2.4:56067787-56067806 | MS.gene059038:CDS | 30.0% | |
| TTTCACAATCACTAACATGA+TGG | + | chr2.4:56067632-56067651 | None:intergenic | 30.0% | |
| TTTGAGTCTAAATTATCTCC+AGG | - | chr2.4:56067786-56067805 | MS.gene059038:CDS | 30.0% | |
| ! | AAAGTATGATTTGCACAAAG+TGG | - | chr2.4:56067680-56067699 | MS.gene059038:intron | 30.0% |
| !!! | AACAGTTTTTTTTACACCGA+CGG | + | chr2.4:56067386-56067405 | None:intergenic | 30.0% |
| ATGTGAAAAATTGAAGTGGC+AGG | - | chr2.4:56067108-56067127 | MS.gene059038:CDS | 35.0% | |
| ATTTAATGGAGATCTACCGT+CGG | - | chr2.4:56067367-56067386 | MS.gene059038:intron | 35.0% | |
| GAAACGAATCAATTATGTGC+AGG | - | chr2.4:56067648-56067667 | MS.gene059038:intron | 35.0% | |
| GACATTCACTTTGTTCAACT+AGG | + | chr2.4:56067610-56067629 | None:intergenic | 35.0% | |
| GGTTATCAGAATCTGTAACT+AGG | + | chr2.4:56066983-56067002 | None:intergenic | 35.0% | |
| TTTCACAGTAAGCTTTGCAA+TGG | + | chr2.4:56067826-56067845 | None:intergenic | 35.0% | |
| ! | AGTAAGTTTCTTGATGGTTC+CGG | + | chr2.4:56067066-56067085 | None:intergenic | 35.0% |
| !! | CATCAACAACCTTTGGAATA+AGG | + | chr2.4:56067004-56067023 | None:intergenic | 35.0% |
| !!! | CCGTCTTTATTTTGAGGATA+TGG | + | chr2.4:56067187-56067206 | None:intergenic | 35.0% |
| ACCAAGTGAAGAGGAAATCA+AGG | - | chr2.4:56067869-56067888 | MS.gene059038:CDS | 40.0% | |
| ACGAATCAATTATGTGCAGG+TGG | - | chr2.4:56067651-56067670 | MS.gene059038:intron | 40.0% | |
| CCAAGTGAAGAGGAAATCAA+GGG | - | chr2.4:56067870-56067889 | MS.gene059038:CDS | 40.0% | |
| GAAATTGTTGAAGGAAACGG+TGG | - | chr2.4:56067038-56067057 | MS.gene059038:CDS | 40.0% | |
| GCTTACAACTACAGCATAGT+TGG | - | chr2.4:56067726-56067745 | MS.gene059038:intron | 40.0% | |
| TACAACTACAGCATAGTTGG+AGG | - | chr2.4:56067729-56067748 | MS.gene059038:intron | 40.0% | |
| AAATCAAGGGTGGCAAAGCT+AGG | - | chr2.4:56067883-56067902 | MS.gene059038:CDS | 45.0% | |
| AAATTATCTCCAGGGCCAGA+TGG | - | chr2.4:56067795-56067814 | MS.gene059038:CDS | 45.0% | |
| AACTACAGCATAGTTGGAGG+TGG | - | chr2.4:56067732-56067751 | MS.gene059038:intron | 45.0% | |
| AATCAAGGGTGGCAAAGCTA+GGG | - | chr2.4:56067884-56067903 | MS.gene059038:CDS | 45.0% | |
| AGCAGGAGCTACAATAGATG+TGG | + | chr2.4:56066946-56066965 | None:intergenic | 45.0% | |
| AGTGAAGAGGAAATCAAGGG+TGG | - | chr2.4:56067873-56067892 | MS.gene059038:CDS | 45.0% | |
| CCCTTGATTTCCTCTTCACT+TGG | + | chr2.4:56067873-56067892 | None:intergenic | 45.0% | |
| TGGATGGCATCAACAACCTT+TGG | + | chr2.4:56067011-56067030 | None:intergenic | 45.0% | |
| TTTGCAATGGATCCTCCATC+TGG | + | chr2.4:56067813-56067832 | None:intergenic | 45.0% | |
| ! | AGGGCTTTGTAAAGTCTAGC+AGG | + | chr2.4:56066963-56066982 | None:intergenic | 45.0% |
| ! | GGCTCTTGAAGGTTACGTTT+TGG | - | chr2.4:56067923-56067942 | MS.gene059038:CDS | 45.0% |
| !! | GGTCTTTTCAAGGCTCTTGA+AGG | - | chr2.4:56067912-56067931 | MS.gene059038:CDS | 45.0% |
| !! | GGTTACGTTTTGGCAAATCC+TGG | - | chr2.4:56067933-56067952 | MS.gene059038:CDS | 45.0% |
| !! | TAGGGGTGATGGTCTTTTCA+AGG | - | chr2.4:56067902-56067921 | MS.gene059038:CDS | 45.0% |
| ATCAAGGGTGGCAAAGCTAG+GGG | - | chr2.4:56067885-56067904 | MS.gene059038:CDS | 50.0% | |
| GAGATCTTCTCCACTGTGTC+TGG | + | chr2.4:56067765-56067784 | None:intergenic | 50.0% | |
| TACAGCATAGTTGGAGGTGG+TGG | - | chr2.4:56067735-56067754 | MS.gene059038:intron | 50.0% | |
| TGATGCTGCACCAAGTGAAG+AGG | - | chr2.4:56067860-56067879 | MS.gene059038:CDS | 50.0% | |
| TTATCTCCAGGGCCAGATGG+AGG | - | chr2.4:56067798-56067817 | MS.gene059038:CDS | 55.0% | |
| !! | TGGTGGACTTCCAGACACAG+TGG | - | chr2.4:56067752-56067771 | MS.gene059038:intron | 55.0% |
| ATGGATCCTCCATCTGGCCC+TGG | + | chr2.4:56067807-56067826 | None:intergenic | 60.0% | |
| GGTGGCAAAGCTAGGGGTGA+TGG | - | chr2.4:56067891-56067910 | MS.gene059038:CDS | 60.0% | |
| GTTGAAGGAAACGGTGGCGC+CGG | - | chr2.4:56067044-56067063 | MS.gene059038:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 56066915 | 56067962 | 56066915 | ID=MS.gene059038 |
| chr2.4 | mRNA | 56066915 | 56067962 | 56066915 | ID=MS.gene059038.t1;Parent=MS.gene059038 |
| chr2.4 | exon | 56067782 | 56067962 | 56067782 | ID=MS.gene059038.t1.exon1;Parent=MS.gene059038.t1 |
| chr2.4 | CDS | 56067782 | 56067962 | 56067782 | ID=cds.MS.gene059038.t1;Parent=MS.gene059038.t1 |
| chr2.4 | exon | 56066915 | 56067207 | 56066915 | ID=MS.gene059038.t1.exon2;Parent=MS.gene059038.t1 |
| chr2.4 | CDS | 56066915 | 56067207 | 56066915 | ID=cds.MS.gene059038.t1;Parent=MS.gene059038.t1 |
| Gene Sequence |
| Protein sequence |
