AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058180


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058180.t1 MTR_2g035100 99.363 157 1 0 1 157 1 157 1.89e-109 308
MS.gene058180.t1 MTR_2g035105 98.089 157 3 0 1 157 1 157 4.99e-108 304
MS.gene058180.t1 MTR_2g035130 87.261 157 20 0 1 157 1 157 1.06e-95 273
MS.gene058180.t1 MTR_2g035150 84.713 157 24 0 1 157 1 157 1.84e-93 268
MS.gene058180.t1 MTR_2g035120 83.439 157 26 0 1 157 1 157 3.36e-93 267
MS.gene058180.t1 MTR_6g033450 71.975 157 44 0 1 157 1 157 2.94e-79 232
MS.gene058180.t1 MTR_4g120970 71.975 157 44 0 1 157 1 157 2.94e-79 232
MS.gene058180.t1 MTR_2g035320 64.968 157 55 0 1 157 1 157 1.37e-68 205
MS.gene058180.t1 MTR_2g035190 61.146 157 61 0 1 157 1 157 2.70e-65 196
MS.gene058180.t1 MTR_2g035210 60.510 157 62 0 1 157 1 157 6.10e-64 193
MS.gene058180.t1 MTR_2g035220 61.146 157 61 0 1 157 1 157 4.01e-63 191
MS.gene058180.t1 MTR_2g035170 59.873 157 63 0 1 157 1 157 3.46e-62 189
MS.gene058180.t1 MTR_2g435310 41.026 156 91 1 1 155 1 156 2.83e-39 130
MS.gene058180.t1 MTR_4g120760 40.385 156 92 1 1 155 1 156 6.60e-37 124
MS.gene058180.t1 MTR_4g120760 40.385 156 92 1 1 155 1 156 6.60e-37 124
MS.gene058180.t1 MTR_2g035320 62.500 96 36 0 62 157 27 122 3.40e-36 121
MS.gene058180.t1 MTR_4g120950 44.099 161 83 5 1 157 1 158 3.35e-32 112
MS.gene058180.t1 MTR_4g120940 61.364 88 31 1 70 157 35 119 1.36e-29 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 42 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCAATAACCTTTGGAATA+AGG 0.200219 2.1:+57715326 None:intergenic
AACGAAAGTAAGTTTCTTGA+TGG 0.287513 2.1:+57715258 None:intergenic
AGCTATATGCTTTAGTAATC+AGG 0.289844 2.1:+57714407 None:intergenic
CCCTTGATTTCCTCTTCACT+TGG 0.329042 2.1:+57714488 None:intergenic
CTTCAACAATTTCAATACTT+TGG 0.342783 2.1:+57715299 None:intergenic
ACTTGGTGCAGCATCACCTT+TGG 0.357443 2.1:+57714505 None:intergenic
GAGATCTTCTCCACAGTGTC+TGG 0.364866 2.1:+57714596 None:intergenic
AAGAAACTTACTTTCGTTGA+AGG 0.370871 2.1:-57715253 MS.gene058180:intron
AGTAAGTTTCTTGATGGTTC+CGG 0.371837 2.1:+57715264 None:intergenic
GGCTCTTGAAGGTTACGTTT+TGG 0.372797 2.1:-57714435 MS.gene058180:CDS
CAAAGTATTGAAATTGTTGA+AGG 0.421501 2.1:-57715298 MS.gene058180:CDS
GCTTACAACTACAGCATAGT+TGG 0.425478 2.1:-57714632 MS.gene058180:CDS
TGGATGGCATCAATAACCTT+TGG 0.428619 2.1:+57715319 None:intergenic
TTTGCAGTGGATCCTCCATC+TGG 0.432310 2.1:+57714548 None:intergenic
AACAATTTCAATACTTTGGA+TGG 0.439896 2.1:+57715303 None:intergenic
ATTGAAATTGTTGAAGGAAA+CGG 0.445615 2.1:-57715292 MS.gene058180:CDS
AAATCAAGGGTGGCAAAGCT+AGG 0.448601 2.1:-57714475 MS.gene058180:CDS
TTTGAGTCTAAATTGTCTGC+AGG 0.453113 2.1:-57714572 MS.gene058180:CDS
CATGGGTGTATTCAACTTTG+AGG 0.475033 2.1:-57715413 MS.gene058180:CDS
AGAGCTTTGTAAAGTCTAGC+AGG 0.480944 2.1:+57715367 None:intergenic
GGTGGCAAAGCTAGGGGTGA+TGG 0.489081 2.1:-57714467 MS.gene058180:CDS
AATCAAGGGTGGCAAAGCTA+GGG 0.491277 2.1:-57714474 MS.gene058180:CDS
ACCAAGTGAAGAGGAAATCA+AGG 0.498836 2.1:-57714489 MS.gene058180:CDS
TACAGCATAGTTGGAGGTGG+TGG 0.504513 2.1:-57714623 MS.gene058180:CDS
TTCTGATAACCTTATTCCAA+AGG 0.526563 2.1:-57715335 MS.gene058180:CDS
AAAGTATGATTTGCACAAAG+TGG 0.527363 2.1:-57714678 MS.gene058180:CDS
GTTGAAGGAAACGGTGGTGC+CGG 0.528042 2.1:-57715283 MS.gene058180:CDS
TGCAGACAATTTAGACTCAA+AGG 0.553148 2.1:+57714574 None:intergenic
AAATTGTCTGCAGGGCCAGA+TGG 0.558648 2.1:-57714563 MS.gene058180:CDS
TTTCACAGTAAGCTTTGCAG+TGG 0.560964 2.1:+57714535 None:intergenic
TTGAGTCTAAATTGTCTGCA+GGG 0.579973 2.1:-57714571 MS.gene058180:CDS
TACAACTACAGCATAGTTGG+AGG 0.596062 2.1:-57714629 MS.gene058180:CDS
TGATGCTGCACCAAGTGAAG+AGG 0.603594 2.1:-57714498 MS.gene058180:CDS
TTGTCTGCAGGGCCAGATGG+AGG 0.618761 2.1:-57714560 MS.gene058180:CDS
AACTACAGCATAGTTGGAGG+TGG 0.623337 2.1:-57714626 MS.gene058180:CDS
ATCAAGGGTGGCAAAGCTAG+GGG 0.634094 2.1:-57714473 MS.gene058180:CDS
AGCAGGAGCTACAATAGATG+TGG 0.641681 2.1:+57715384 None:intergenic
ACTGTGAAATACTTCACCAA+AGG 0.653868 2.1:-57714521 MS.gene058180:CDS
CCAAGTGAAGAGGAAATCAA+GGG 0.664848 2.1:-57714488 MS.gene058180:CDS
AGTGAAGAGGAAATCAAGGG+TGG 0.695634 2.1:-57714485 MS.gene058180:CDS
TGGTGGACTTCCAGACACTG+TGG 0.725966 2.1:-57714606 MS.gene058180:CDS
GAAATTGTTGAAGGAAACGG+TGG 0.726108 2.1:-57715289 MS.gene058180:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATAATAAAAGAAAAG+AGG + chr2.1:57714960-57714979 None:intergenic 10.0%
!!! TCTTTTCTTTTATTATATAT+TGG - chr2.1:57714959-57714978 MS.gene058180:intron 10.0%
!!! CTTTTAAAAAATAGTTTTTC+TGG + chr2.1:57714894-57714913 None:intergenic 15.0%
!!! GTGTTATTATATACATTTAA+TGG - chr2.1:57714825-57714844 MS.gene058180:intron 15.0%
!! AAGATACATAACATAAATTG+TGG - chr2.1:57714755-57714774 MS.gene058180:intron 20.0%
!! ATATATGTGAAAAATTGAAG+TGG - chr2.1:57714607-57714626 MS.gene058180:CDS 20.0%
! AACAAGAATCAAACATTTGA+TGG + chr2.1:57714920-57714939 None:intergenic 25.0%
! AACAATTTCAATACTTTGGA+TGG + chr2.1:57714530-57714549 None:intergenic 25.0%
! ATACATAACATAAATTGTGG+AGG - chr2.1:57714758-57714777 MS.gene058180:intron 25.0%
! ATTGAAATTGTTGAAGGAAA+CGG - chr2.1:57714538-57714557 MS.gene058180:CDS 25.0%
! CTTCAACAATTTCAATACTT+TGG + chr2.1:57714534-57714553 None:intergenic 25.0%
! TTTCACAATTACTAACATGA+TGG + chr2.1:57715104-57715123 None:intergenic 25.0%
!!! AATTAACCGTCTTTATTTTG+AGG + chr2.1:57714696-57714715 None:intergenic 25.0%
!!! CAAAGTATTGAAATTGTTGA+AGG - chr2.1:57714532-57714551 MS.gene058180:CDS 25.0%
AACGAAAGTAAGTTTCTTGA+TGG + chr2.1:57714575-57714594 None:intergenic 30.0%
AAGAAACTTACTTTCGTTGA+AGG - chr2.1:57714577-57714596 MS.gene058180:CDS 30.0%
CCATATCCTCAAAATAAAGA+CGG - chr2.1:57714687-57714706 MS.gene058180:CDS 30.0%
TTCATGCAGTGAAAATTGTT+TGG + chr2.1:57714665-57714684 None:intergenic 30.0%
TTCTGATAACCTTATTCCAA+AGG - chr2.1:57714495-57714514 MS.gene058180:CDS 30.0%
! AAAGTATGATTTGCACAAAG+TGG - chr2.1:57715152-57715171 MS.gene058180:intron 30.0%
!! CATCAATAACCTTTGGAATA+AGG + chr2.1:57714507-57714526 None:intergenic 30.0%
!!! AACAGTTTTTTTTACACCGA+CGG + chr2.1:57714858-57714877 None:intergenic 30.0%
ACTGTGAAATACTTCACCAA+AGG - chr2.1:57715309-57715328 MS.gene058180:CDS 35.0%
ATGTGAAAAATTGAAGTGGC+AGG - chr2.1:57714611-57714630 MS.gene058180:CDS 35.0%
ATTTAATGGAGATCTACCGT+CGG - chr2.1:57714839-57714858 MS.gene058180:intron 35.0%
GAAACGAATCAATTATGTGC+AGG - chr2.1:57715120-57715139 MS.gene058180:intron 35.0%
TGCAGACAATTTAGACTCAA+AGG + chr2.1:57715259-57715278 None:intergenic 35.0%
TTGAGTCTAAATTGTCTGCA+GGG - chr2.1:57715259-57715278 MS.gene058180:CDS 35.0%
TTTGAGTCTAAATTGTCTGC+AGG - chr2.1:57715258-57715277 MS.gene058180:CDS 35.0%
! AGTAAGTTTCTTGATGGTTC+CGG + chr2.1:57714569-57714588 None:intergenic 35.0%
!! TAACATGATGGACATTCACT+TGG + chr2.1:57715092-57715111 None:intergenic 35.0%
!!! CCGTCTTTATTTTGAGGATA+TGG + chr2.1:57714690-57714709 None:intergenic 35.0%
ACCAAGTGAAGAGGAAATCA+AGG - chr2.1:57715341-57715360 MS.gene058180:CDS 40.0%
ACGAATCAATTATGTGCAGG+TGG - chr2.1:57715123-57715142 MS.gene058180:intron 40.0%
CCAAGTGAAGAGGAAATCAA+GGG - chr2.1:57715342-57715361 MS.gene058180:CDS 40.0%
GAAATTGTTGAAGGAAACGG+TGG - chr2.1:57714541-57714560 MS.gene058180:CDS 40.0%
GACATTCACTTGGTTCAACT+AGG + chr2.1:57715082-57715101 None:intergenic 40.0%
GCTTACAACTACAGCATAGT+TGG - chr2.1:57715198-57715217 MS.gene058180:intron 40.0%
TACAACTACAGCATAGTTGG+AGG - chr2.1:57715201-57715220 MS.gene058180:intron 40.0%
TGGATGGCATCAATAACCTT+TGG + chr2.1:57714514-57714533 None:intergenic 40.0%
TTTCACAGTAAGCTTTGCAG+TGG + chr2.1:57715298-57715317 None:intergenic 40.0%
! AGAGCTTTGTAAAGTCTAGC+AGG + chr2.1:57714466-57714485 None:intergenic 40.0%
AAATCAAGGGTGGCAAAGCT+AGG - chr2.1:57715355-57715374 MS.gene058180:CDS 45.0%
AACTACAGCATAGTTGGAGG+TGG - chr2.1:57715204-57715223 MS.gene058180:intron 45.0%
AATCAAGGGTGGCAAAGCTA+GGG - chr2.1:57715356-57715375 MS.gene058180:CDS 45.0%
AGCAGGAGCTACAATAGATG+TGG + chr2.1:57714449-57714468 None:intergenic 45.0%
AGTGAAGAGGAAATCAAGGG+TGG - chr2.1:57715345-57715364 MS.gene058180:CDS 45.0%
CCCTTGATTTCCTCTTCACT+TGG + chr2.1:57715345-57715364 None:intergenic 45.0%
! GGCTCTTGAAGGTTACGTTT+TGG - chr2.1:57715395-57715414 MS.gene058180:CDS 45.0%
!! GGTCTTTTCAAGGCTCTTGA+AGG - chr2.1:57715384-57715403 MS.gene058180:CDS 45.0%
!! TAGGGGTGATGGTCTTTTCA+AGG - chr2.1:57715374-57715393 MS.gene058180:CDS 45.0%
AAATTGTCTGCAGGGCCAGA+TGG - chr2.1:57715267-57715286 MS.gene058180:CDS 50.0%
ATCAAGGGTGGCAAAGCTAG+GGG - chr2.1:57715357-57715376 MS.gene058180:CDS 50.0%
GAGATCTTCTCCACAGTGTC+TGG + chr2.1:57715237-57715256 None:intergenic 50.0%
TACAGCATAGTTGGAGGTGG+TGG - chr2.1:57715207-57715226 MS.gene058180:intron 50.0%
TGATGCTGCACCAAGTGAAG+AGG - chr2.1:57715332-57715351 MS.gene058180:CDS 50.0%
TTTGCAGTGGATCCTCCATC+TGG + chr2.1:57715285-57715304 None:intergenic 50.0%
!! ACTTGGTGCAGCATCACCTT+TGG + chr2.1:57715328-57715347 None:intergenic 50.0%
GTTGAAGGAAACGGTGGTGC+CGG - chr2.1:57714547-57714566 MS.gene058180:CDS 55.0%
!! TGGTGGACTTCCAGACACTG+TGG - chr2.1:57715224-57715243 MS.gene058180:intron 55.0%
GGTGGCAAAGCTAGGGGTGA+TGG - chr2.1:57715363-57715382 MS.gene058180:CDS 60.0%
TTGTCTGCAGGGCCAGATGG+AGG - chr2.1:57715270-57715289 MS.gene058180:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 57714418 57715434 57714418 ID=MS.gene058180
chr2.1 mRNA 57714418 57715434 57714418 ID=MS.gene058180.t1;Parent=MS.gene058180
chr2.1 exon 57715254 57715434 57715254 ID=MS.gene058180.t1.exon1;Parent=MS.gene058180.t1
chr2.1 CDS 57715254 57715434 57715254 ID=cds.MS.gene058180.t1;Parent=MS.gene058180.t1
chr2.1 exon 57714418 57714710 57714418 ID=MS.gene058180.t1.exon2;Parent=MS.gene058180.t1
chr2.1 CDS 57714418 57714710 57714418 ID=cds.MS.gene058180.t1;Parent=MS.gene058180.t1
Gene Sequence

>MS.gene058180

ATGGGTGTATTCAACTTTGAGGATGAGACCACATCTATTGTAGCTCCTGCTAGACTTTACAAAGCTCTAGTTACAGATTCTGATAACCTTATTCCAAAGGTTATTGATGCCATCCAAAGTATTGAAATTGTTGAAGGAAACGGTGGTGCCGGAACCATCAAGAAACTTACTTTCGTTGAAGGTTAGTATATATATGTGAAAAATTGAAGTGGCAGGAAGTAGAATAAAATGTTTAACAACAAATCCAAACAATTTTCACTGCATGAAATCCATATCCTCAAAATAAAGACGGTTAATTTAATTTAATTTTTTTTTTATCTAAATATAGAGCATGTTTAAGATACATAACATAAATTGTGGAGGTCAACAAAGCACATGAGAATATTTTTTTGATGACATCATTAACAGTGTTATTATATACATTTAATGGAGATCTACCGTCGGTGTAAAAAAAACTGTTTTTTAAAACTTGACCAGAAAAACTATTTTTTAAAAGTAACCATCAAATGTTTGATTCTTGTTGATTGTGAAGTGCATATCCTCTTTTCTTTTATTATATATTGGATTATTTGTTACTATGTTTGTTATATAATATTTTTTTATGAGAAACAATAGTATTTTTAATTTTTTTTTAATAGCTTCTTATACAAATTTTTAATAACCTAGTTGAACCAAGTGAATGTCCATCATGTTAGTAATTGTGAAACGAATCAATTATGTGCAGGTGGTGAAACAAAGTATGATTTGCACAAAGTGGACTTAGTAGATGATGTTAACTTTGCTTACAACTACAGCATAGTTGGAGGTGGTGGACTTCCAGACACTGTGGAGAAGATCTCCTTTGAGTCTAAATTGTCTGCAGGGCCAGATGGAGGATCCACTGCAAAGCTTACTGTGAAATACTTCACCAAAGGTGATGCTGCACCAAGTGAAGAGGAAATCAAGGGTGGCAAAGCTAGGGGTGATGGTCTTTTCAAGGCTCTTGAAGGTTACGTTTTGGCAAATCCTGATTACTAA

Protein sequence

>MS.gene058180.t1

MGVFNFEDETTSIVAPARLYKALVTDSDNLIPKVIDAIQSIEIVEGNGGAGTIKKLTFVEGGETKYDLHKVDLVDDVNFAYNYSIVGGGGLPDTVEKISFESKLSAGPDGGSTAKLTVKYFTKGDAAPSEEEIKGGKARGDGLFKALEGYVLANPDY