Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41870.t1 | XP_003624840.1 | 93.7 | 366 | 23 | 0 | 4 | 369 | 24 | 389 | 1.70E-163 | 585.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41870.t1 | Q8L727 | 32.5 | 228 | 129 | 5 | 58 | 261 | 246 | 472 | 5.7e-22 | 106.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41870.t1 | G7L3S4 | 93.7 | 366 | 23 | 0 | 4 | 369 | 24 | 389 | 1.3e-163 | 585.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049341 | MS.gene41870 | 0.804536 | 2.06E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene41870 | 0.805696 | 1.18E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene41870 | 0.800491 | 1.41E-48 | -1.69E-46 |
| MS.gene050838 | MS.gene41870 | 0.805804 | 1.12E-49 | -1.69E-46 |
| MS.gene051687 | MS.gene41870 | 0.823979 | 1.03E-53 | -1.69E-46 |
| MS.gene051885 | MS.gene41870 | 0.802494 | 5.46E-49 | -1.69E-46 |
| MS.gene052133 | MS.gene41870 | 0.852044 | 5.97E-61 | -1.69E-46 |
| MS.gene052947 | MS.gene41870 | 0.81399 | 1.93E-51 | -1.69E-46 |
| MS.gene053437 | MS.gene41870 | 0.82086 | 5.49E-53 | -1.69E-46 |
| MS.gene055465 | MS.gene41870 | 0.807543 | 4.80E-50 | -1.69E-46 |
| MS.gene055468 | MS.gene41870 | 0.813236 | 2.83E-51 | -1.69E-46 |
| MS.gene055580 | MS.gene41870 | -0.81206 | 5.13E-51 | -1.69E-46 |
| MS.gene055891 | MS.gene41870 | 0.822271 | 2.59E-53 | -1.69E-46 |
| MS.gene056043 | MS.gene41870 | -0.818192 | 2.23E-52 | -1.69E-46 |
| MS.gene058852 | MS.gene41870 | 0.819062 | 1.41E-52 | -1.69E-46 |
| MS.gene058906 | MS.gene41870 | 0.811534 | 6.67E-51 | -1.69E-46 |
| MS.gene059171 | MS.gene41870 | 0.830284 | 3.20E-55 | -1.69E-46 |
| MS.gene06001 | MS.gene41870 | 0.821587 | 3.73E-53 | -1.69E-46 |
| MS.gene060587 | MS.gene41870 | 0.80455 | 2.05E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene41870 | MS.gene066439 | PPI |
| MS.gene41870 | MS.gene90825 | PPI |
| MS.gene41870 | MS.gene97346 | PPI |
| MS.gene41870 | MS.gene029093 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41870.t1 | MTR_7g088160 | 93.443 | 366 | 24 | 0 | 4 | 369 | 24 | 389 | 0.0 | 720 |
| MS.gene41870.t1 | MTR_7g107340 | 33.613 | 357 | 176 | 7 | 15 | 368 | 36 | 334 | 3.16e-56 | 187 |
| MS.gene41870.t1 | MTR_4g063670 | 40.125 | 319 | 165 | 8 | 56 | 368 | 21 | 319 | 5.20e-56 | 186 |
| MS.gene41870.t1 | MTR_0867s0010 | 38.050 | 318 | 175 | 8 | 55 | 368 | 20 | 319 | 1.92e-52 | 177 |
| MS.gene41870.t1 | MTR_4g083510 | 38.245 | 319 | 174 | 9 | 55 | 368 | 20 | 320 | 8.47e-49 | 167 |
| MS.gene41870.t1 | MTR_1g051185 | 47.183 | 142 | 72 | 3 | 99 | 238 | 41 | 181 | 1.99e-32 | 122 |
| MS.gene41870.t1 | MTR_3g069460 | 42.484 | 153 | 84 | 3 | 56 | 204 | 21 | 173 | 2.99e-31 | 117 |
| MS.gene41870.t1 | MTR_7g114170 | 41.714 | 175 | 68 | 4 | 48 | 221 | 56 | 197 | 6.14e-30 | 116 |
| MS.gene41870.t1 | MTR_6g081150 | 32.692 | 208 | 75 | 4 | 52 | 259 | 14 | 156 | 1.05e-26 | 105 |
| MS.gene41870.t1 | MTR_8g015750 | 32.870 | 216 | 111 | 7 | 151 | 366 | 3 | 184 | 9.51e-19 | 83.6 |
| MS.gene41870.t1 | MTR_2g072860 | 26.257 | 179 | 126 | 2 | 33 | 205 | 7 | 185 | 2.62e-18 | 84.7 |
| MS.gene41870.t1 | MTR_7g461350 | 27.119 | 177 | 123 | 2 | 33 | 203 | 75 | 251 | 6.23e-18 | 84.7 |
| MS.gene41870.t1 | MTR_6g016670 | 27.119 | 177 | 123 | 2 | 33 | 203 | 75 | 251 | 6.23e-18 | 84.7 |
| MS.gene41870.t1 | MTR_3g464310 | 27.119 | 177 | 123 | 2 | 33 | 203 | 75 | 251 | 6.23e-18 | 84.7 |
| MS.gene41870.t1 | MTR_3g450670 | 27.119 | 177 | 123 | 2 | 33 | 203 | 71 | 247 | 8.90e-18 | 84.0 |
| MS.gene41870.t1 | MTR_1g471070 | 27.119 | 177 | 123 | 2 | 33 | 203 | 71 | 247 | 8.90e-18 | 84.0 |
| MS.gene41870.t1 | MTR_3g464170 | 26.554 | 177 | 124 | 2 | 33 | 203 | 75 | 251 | 1.18e-17 | 84.0 |
| MS.gene41870.t1 | MTR_1g100375 | 26.554 | 177 | 124 | 2 | 33 | 203 | 75 | 251 | 1.18e-17 | 84.0 |
| MS.gene41870.t1 | MTR_3g067785 | 28.194 | 227 | 142 | 6 | 13 | 222 | 12 | 234 | 1.41e-17 | 83.2 |
| MS.gene41870.t1 | MTR_4g013220 | 28.194 | 227 | 142 | 6 | 13 | 222 | 12 | 234 | 1.63e-17 | 82.8 |
| MS.gene41870.t1 | MTR_6g051405 | 26.554 | 177 | 124 | 2 | 33 | 203 | 75 | 251 | 1.78e-17 | 83.2 |
| MS.gene41870.t1 | MTR_4g026120 | 28.194 | 227 | 142 | 6 | 13 | 222 | 12 | 234 | 1.97e-17 | 82.8 |
| MS.gene41870.t1 | MTR_4g036435 | 28.194 | 227 | 142 | 6 | 13 | 222 | 12 | 234 | 1.97e-17 | 82.8 |
| MS.gene41870.t1 | MTR_6g026810 | 71.154 | 52 | 14 | 1 | 4 | 55 | 44 | 94 | 2.35e-17 | 79.0 |
| MS.gene41870.t1 | MTR_5g066280 | 28.194 | 227 | 142 | 6 | 13 | 222 | 12 | 234 | 2.91e-17 | 82.0 |
| MS.gene41870.t1 | MTR_2g022120 | 27.753 | 227 | 143 | 6 | 13 | 222 | 76 | 298 | 1.35e-16 | 80.9 |
| MS.gene41870.t1 | MTR_1g106885 | 27.753 | 227 | 143 | 6 | 13 | 222 | 73 | 295 | 1.60e-16 | 80.5 |
| MS.gene41870.t1 | MTR_5g077245 | 27.313 | 227 | 144 | 6 | 13 | 222 | 65 | 287 | 2.52e-16 | 80.1 |
| MS.gene41870.t1 | MTR_6g092533 | 28.218 | 202 | 130 | 5 | 13 | 203 | 76 | 273 | 4.77e-16 | 79.3 |
| MS.gene41870.t1 | MTR_4g084190 | 64.151 | 53 | 18 | 1 | 4 | 56 | 73 | 124 | 3.40e-15 | 73.6 |
| MS.gene41870.t1 | MTR_4g084190 | 62.745 | 51 | 18 | 1 | 4 | 54 | 73 | 122 | 1.00e-14 | 70.9 |
| MS.gene41870.t1 | MTR_6g453050 | 25.405 | 185 | 124 | 3 | 52 | 222 | 50 | 234 | 1.12e-14 | 74.7 |
| MS.gene41870.t1 | MTR_4g084190 | 64.000 | 50 | 17 | 1 | 4 | 53 | 73 | 121 | 4.20e-14 | 70.5 |
| MS.gene41870.t1 | MTR_1g086750 | 28.571 | 126 | 90 | 0 | 77 | 202 | 18 | 143 | 1.72e-13 | 69.3 |
| MS.gene41870.t1 | MTR_4g046090 | 32.500 | 120 | 75 | 1 | 109 | 222 | 75 | 194 | 7.43e-13 | 68.6 |
| MS.gene41870.t1 | MTR_7g062380 | 26.316 | 152 | 106 | 1 | 77 | 222 | 18 | 169 | 4.59e-12 | 66.2 |
| MS.gene41870.t1 | MTR_7g083520 | 29.231 | 130 | 87 | 2 | 76 | 201 | 53 | 181 | 5.57e-12 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41870.t1 | AT5G41240 | 31.599 | 269 | 158 | 6 | 58 | 301 | 246 | 513 | 2.57e-27 | 113 |
| MS.gene41870.t1 | AT5G41220 | 36.158 | 177 | 96 | 5 | 69 | 228 | 249 | 425 | 5.70e-24 | 103 |
| MS.gene41870.t1 | AT5G41220 | 36.158 | 177 | 96 | 5 | 69 | 228 | 199 | 375 | 5.81e-24 | 103 |
| MS.gene41870.t1 | AT5G41220 | 36.158 | 177 | 96 | 5 | 69 | 228 | 191 | 367 | 5.96e-24 | 103 |
| MS.gene41870.t1 | AT3G47680 | 39.370 | 127 | 74 | 2 | 79 | 203 | 44 | 169 | 2.13e-21 | 93.6 |
| MS.gene41870.t1 | AT3G56490 | 71.154 | 52 | 14 | 1 | 4 | 55 | 38 | 88 | 2.19e-17 | 78.6 |
| MS.gene41870.t1 | AT1G31160 | 63.462 | 52 | 18 | 1 | 4 | 55 | 78 | 128 | 1.81e-14 | 71.6 |
| MS.gene41870.t1 | AT1G31160 | 61.538 | 52 | 19 | 1 | 4 | 55 | 78 | 128 | 2.93e-14 | 70.1 |
Find 84 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCGGAATTATTACAATA+TGG | 0.179127 | 7.4:+20182329 | None:intergenic |
| TTTGGCAGGTCCTTGCCTTT+AGG | 0.180256 | 7.4:-20182375 | MS.gene41870:intron |
| TTGGCAGGTCCTTGCCTTTA+GGG | 0.212853 | 7.4:-20182374 | MS.gene41870:intron |
| AATGATGTATTTGACCATTT+AGG | 0.247849 | 7.4:+20176897 | None:intergenic |
| TGAGTTGGAGTTCCTCTTTA+AGG | 0.259351 | 7.4:-20176493 | MS.gene41870:CDS |
| TGCCTCCGCAGCTGCATTCT+TGG | 0.287331 | 7.4:+20177196 | None:intergenic |
| ATCAAAGAGTAGATTCCTTT+TGG | 0.297331 | 7.4:-20177172 | MS.gene41870:CDS |
| ACAATATGGGGAGGAGCTTT+AGG | 0.298958 | 7.4:+20182343 | None:intergenic |
| TGACAGGAGTATTATCAAAA+AGG | 0.309485 | 7.4:-20183842 | MS.gene41870:CDS |
| TGTTGAAAGATGAACCTAAA+TGG | 0.314612 | 7.4:-20176911 | MS.gene41870:CDS |
| GCCTCCGCAGCTGCATTCTT+GGG | 0.331691 | 7.4:+20177197 | None:intergenic |
| CCTACAGTGGTTTGTCTAAC+GGG | 0.353092 | 7.4:-20176774 | MS.gene41870:CDS |
| TCCTCATAAAACACAGTAGA+AGG | 0.377289 | 7.4:+20183814 | None:intergenic |
| TTATTTATTATGTGATGAAC+AGG | 0.380340 | 7.4:-20183867 | None:intergenic |
| AGCATAGTTCATAAATTTGT+AGG | 0.381452 | 7.4:-20177059 | MS.gene41870:CDS |
| GTAATATTATTCAACCCAAC+TGG | 0.383376 | 7.4:+20177314 | None:intergenic |
| AGAACCAAGATTTCTGCTTC+AGG | 0.391537 | 7.4:-20176858 | MS.gene41870:CDS |
| CTCATGATCTCGTTGTTGTC+TGG | 0.395467 | 7.4:+20176452 | None:intergenic |
| TTTCGGAATTATTACAATAT+GGG | 0.399163 | 7.4:+20182330 | None:intergenic |
| TTCCTCTTTAAGGATATATC+CGG | 0.407777 | 7.4:-20176483 | MS.gene41870:CDS |
| GTCAACCCATCTCTAGCTTT+CGG | 0.412932 | 7.4:+20182313 | None:intergenic |
| AGTTCTCGTCATGGCAGAAA+TGG | 0.413601 | 7.4:-20176580 | MS.gene41870:CDS |
| TGCTCCTGAAGCAGAAATCT+TGG | 0.437645 | 7.4:+20176854 | None:intergenic |
| GCGTTGTATTCACAACTTGA+TGG | 0.441509 | 7.4:+20176804 | None:intergenic |
| ATTCTTAATTCTTAGCCAAA+AGG | 0.441749 | 7.4:+20177157 | None:intergenic |
| ATACTCGACCACTTCTAATC+TGG | 0.446723 | 7.4:-20176832 | MS.gene41870:CDS |
| AAGAAAGGGAAAATCAAAAT+CGG | 0.456154 | 7.4:-20176736 | MS.gene41870:CDS |
| GGAGAAAGAGTTGAATCTAA+AGG | 0.457496 | 7.4:-20176526 | MS.gene41870:CDS |
| ACACTCCCCTCGGTTTGAAA+TGG | 0.473048 | 7.4:+20177114 | None:intergenic |
| GACAGACGTAGAGGAAAGAA+AGG | 0.477930 | 7.4:-20176751 | MS.gene41870:CDS |
| ATGTGATGAACAGGTTTGAC+AGG | 0.487322 | 7.4:-20183858 | None:intergenic |
| TTGTTGTCTGGCTGTCATCC+CGG | 0.489120 | 7.4:+20176464 | None:intergenic |
| ACACTTTATTATCAAGATGA+AGG | 0.490019 | 7.4:-20176966 | MS.gene41870:CDS |
| AAAGGAGAGAGAGTTTGAGT+TGG | 0.492472 | 7.4:-20176508 | MS.gene41870:CDS |
| TGGGTTGACTGGTCTATCTA+AGG | 0.493311 | 7.4:-20182299 | MS.gene41870:intron |
| AGATGAACAAAATAAGTTGA+AGG | 0.494225 | 7.4:-20176547 | MS.gene41870:CDS |
| TAGCTCTGAGTCTGACATCA+TGG | 0.497740 | 7.4:-20177000 | MS.gene41870:CDS |
| ATGAGAACTTTATCTTCCCC+AGG | 0.502700 | 7.4:+20177239 | None:intergenic |
| TTCGGAATTATTACAATATG+GGG | 0.506959 | 7.4:+20182331 | None:intergenic |
| TGGGAACGATGAGCCATGCT+TGG | 0.513548 | 7.4:+20177216 | None:intergenic |
| TGGTCAAATACATCATTTGA+AGG | 0.523432 | 7.4:-20176891 | MS.gene41870:CDS |
| CAACTGGGCTTGAAAACTGT+GGG | 0.529312 | 7.4:+20177330 | None:intergenic |
| TTTAGGAGCTATATCCCTAA+AGG | 0.535725 | 7.4:+20182360 | None:intergenic |
| TTTATTTGTGACACTCCCCT+CGG | 0.536783 | 7.4:+20177104 | None:intergenic |
| ACCTACAGTGGTTTGTCTAA+CGG | 0.538338 | 7.4:-20176775 | MS.gene41870:CDS |
| GCATAGTTCATAAATTTGTA+GGG | 0.549641 | 7.4:-20177058 | MS.gene41870:CDS |
| AAAGAACAAGGTTTACCCCT+GGG | 0.552641 | 7.4:-20177256 | MS.gene41870:CDS |
| TCAAACAGAGGCAGAAGTAG+AGG | 0.552685 | 7.4:-20176685 | MS.gene41870:CDS |
| AAAATCGGAAGAAACATCTT+CGG | 0.555404 | 7.4:-20176721 | MS.gene41870:CDS |
| AAAGCTAGAGATGGGTTGAC+TGG | 0.562006 | 7.4:-20182310 | MS.gene41870:CDS |
| TCCCGGATATATCCTTAAAG+AGG | 0.562142 | 7.4:+20176481 | None:intergenic |
| TCCCAAGAATGCAGCTGCGG+AGG | 0.562688 | 7.4:-20177198 | MS.gene41870:CDS |
| TCCTCTTTAAGGATATATCC+GGG | 0.564537 | 7.4:-20176482 | MS.gene41870:CDS |
| TAATATTATTCAACCCAACT+GGG | 0.569197 | 7.4:+20177315 | None:intergenic |
| TCGCGGCCATTTCAAACCGA+GGG | 0.574944 | 7.4:-20177120 | MS.gene41870:CDS |
| AGCTCTGAGTCTGACATCAT+GGG | 0.578396 | 7.4:-20176999 | MS.gene41870:CDS |
| ATCGCGGCCATTTCAAACCG+AGG | 0.583777 | 7.4:-20177121 | MS.gene41870:CDS |
| ATAATTCCGAAAGCTAGAGA+TGG | 0.589633 | 7.4:-20182319 | MS.gene41870:CDS |
| GGAATTATTACAATATGGGG+AGG | 0.589697 | 7.4:+20182334 | None:intergenic |
| TAATTCCGAAAGCTAGAGAT+GGG | 0.593326 | 7.4:-20182318 | MS.gene41870:CDS |
| AGTTCAAGTTAGAGTATGCA+TGG | 0.599942 | 7.4:-20176938 | MS.gene41870:CDS |
| TGAGAACTTTATCTTCCCCA+GGG | 0.604635 | 7.4:+20177240 | None:intergenic |
| GAGCTATATCCCTAAAGGCA+AGG | 0.606414 | 7.4:+20182365 | None:intergenic |
| AAAAGAACAAGGTTTACCCC+TGG | 0.612783 | 7.4:-20177257 | MS.gene41870:CDS |
| ATAAAGTTCTCATCCAAGCA+TGG | 0.618728 | 7.4:-20177229 | MS.gene41870:CDS |
| ACAGAGGCAGAAGTAGAGGA+AGG | 0.621119 | 7.4:-20176681 | MS.gene41870:CDS |
| CCAACTGGGCTTGAAAACTG+TGG | 0.629875 | 7.4:+20177329 | None:intergenic |
| AAAGACAAAAGTTCTCGTCA+TGG | 0.630789 | 7.4:-20176589 | MS.gene41870:CDS |
| GGGTTGAATAATATTACACT+CGG | 0.631705 | 7.4:-20177308 | MS.gene41870:CDS |
| ACAGACGTAGAGGAAAGAAA+GGG | 0.633644 | 7.4:-20176750 | MS.gene41870:CDS |
| CCCGTTAGACAAACCACTGT+AGG | 0.634378 | 7.4:+20176774 | None:intergenic |
| CGTTCCCAAGAATGCAGCTG+CGG | 0.637770 | 7.4:-20177201 | MS.gene41870:CDS |
| TGGCATGTCCAGATTAGAAG+TGG | 0.642053 | 7.4:+20176824 | None:intergenic |
| AATAATTACAACGAGCATCG+CGG | 0.652454 | 7.4:-20177137 | MS.gene41870:CDS |
| GCTTTGTCTTTGTCAAACAG+AGG | 0.658520 | 7.4:-20176697 | MS.gene41870:CDS |
| CAACGCAGCATCACCTACAG+TGG | 0.666435 | 7.4:-20176787 | MS.gene41870:CDS |
| TGTCACAAATAAAATCGCGA+TGG | 0.669419 | 7.4:-20177094 | MS.gene41870:CDS |
| CTACAGTGGTTTGTCTAACG+GGG | 0.672077 | 7.4:-20176773 | MS.gene41870:CDS |
| TCAAATACATCATTTGAAGG+AGG | 0.672832 | 7.4:-20176888 | MS.gene41870:CDS |
| CGCGGCCATTTCAAACCGAG+GGG | 0.678447 | 7.4:-20177119 | MS.gene41870:CDS |
| TCTAACGGGGACAGACGTAG+AGG | 0.684957 | 7.4:-20176760 | MS.gene41870:CDS |
| AACTGGGCTTGAAAACTGTG+GGG | 0.689956 | 7.4:+20177331 | None:intergenic |
| AAGAACAAGGTTTACCCCTG+GGG | 0.699394 | 7.4:-20177255 | MS.gene41870:CDS |
| GAGAACTTTATCTTCCCCAG+GGG | 0.729630 | 7.4:+20177241 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 20176410 | 20183875 | 20176410 | ID=MS.gene41870 |
| chr7.4 | mRNA | 20176410 | 20183875 | 20176410 | ID=MS.gene41870.t1;Parent=MS.gene41870 |
| chr7.4 | exon | 20183807 | 20183875 | 20183807 | ID=MS.gene41870.t1.exon1;Parent=MS.gene41870.t1 |
| chr7.4 | CDS | 20183807 | 20183875 | 20183807 | ID=cds.MS.gene41870.t1;Parent=MS.gene41870.t1 |
| chr7.4 | exon | 20182300 | 20182389 | 20182300 | ID=MS.gene41870.t1.exon2;Parent=MS.gene41870.t1 |
| chr7.4 | CDS | 20182300 | 20182389 | 20182300 | ID=cds.MS.gene41870.t1;Parent=MS.gene41870.t1 |
| chr7.4 | exon | 20176410 | 20177360 | 20176410 | ID=MS.gene41870.t1.exon3;Parent=MS.gene41870.t1 |
| chr7.4 | CDS | 20176410 | 20177360 | 20176410 | ID=cds.MS.gene41870.t1;Parent=MS.gene41870.t1 |
| Gene Sequence |
| Protein sequence |
