AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48249

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48249.t1 MTR_4g025730 99.545 440 2 0 1 440 70 509 0.0 907
MS.gene48249.t1 MTR_4g025690 76.351 444 98 4 2 440 72 513 0.0 700
MS.gene48249.t1 MTR_8g104655 53.333 450 181 8 19 440 93 541 7.07e-166 478
MS.gene48249.t1 MTR_7g108905 51.496 468 195 10 1 436 85 552 7.70e-160 463
MS.gene48249.t1 MTR_4g035980 51.082 462 203 9 1 440 79 539 2.22e-155 451
MS.gene48249.t1 MTR_3g033690 50.877 456 206 9 1 440 73 526 3.78e-153 445
MS.gene48249.t1 MTR_3g008640 52.304 434 189 9 23 440 96 527 2.20e-152 443
MS.gene48249.t1 MTR_3g008720 52.074 434 190 9 23 440 96 527 1.63e-151 441
MS.gene48249.t1 MTR_7g053360 52.074 434 190 9 23 440 96 527 1.98e-151 440
MS.gene48249.t1 MTR_3g008700 52.074 434 190 9 23 440 96 527 1.48e-150 438
MS.gene48249.t1 MTR_8g104645 60.882 340 123 5 111 440 33 372 1.07e-148 427
MS.gene48249.t1 MTR_7g050870 50.109 459 202 10 5 440 100 554 7.08e-146 427
MS.gene48249.t1 MTR_4g025670 51.740 431 186 9 26 440 65 489 1.45e-144 421
MS.gene48249.t1 MTR_7g112580 49.443 449 189 10 22 440 102 542 5.80e-143 419
MS.gene48249.t1 MTR_8g023310 49.786 468 192 11 14 440 124 589 8.72e-143 421
MS.gene48249.t1 MTR_7g050980 50.334 449 192 11 17 440 113 555 2.07e-142 418
MS.gene48249.t1 MTR_4g087980 51.025 439 183 9 26 440 86 516 5.14e-142 416
MS.gene48249.t1 MTR_2g044880 50.228 438 191 8 26 440 92 525 3.64e-140 412
MS.gene48249.t1 MTR_8g104580 47.661 449 175 9 19 440 85 500 4.31e-140 410
MS.gene48249.t1 MTR_1g083520 50.794 441 183 10 29 440 93 528 1.89e-138 407
MS.gene48249.t1 MTR_1g088020 48.760 484 192 14 10 440 75 555 8.15e-137 404
MS.gene48249.t1 MTR_7g050950 47.939 461 208 12 5 440 100 553 8.83e-137 404
MS.gene48249.t1 MTR_3g091380 46.537 462 204 9 20 440 138 597 1.29e-136 405
MS.gene48249.t1 MTR_1g054880 49.677 465 182 14 26 440 96 558 1.32e-135 401
MS.gene48249.t1 MTR_1g008140 46.039 467 204 11 21 440 142 607 9.15e-135 400
MS.gene48249.t1 MTR_7g108900 48.077 468 188 13 26 440 95 560 3.85e-133 395
MS.gene48249.t1 MTR_1g088225 48.347 484 192 15 10 440 75 553 1.09e-132 393
MS.gene48249.t1 MTR_7g108875 48.180 467 187 13 26 440 101 564 8.38e-130 386
MS.gene48249.t1 MTR_7g108890 49.417 429 175 9 53 440 135 562 2.02e-129 385
MS.gene48249.t1 MTR_3g010780 47.072 461 205 11 10 440 123 574 2.43e-129 385
MS.gene48249.t1 MTR_8g042910 47.158 475 192 12 23 440 140 612 7.30e-128 383
MS.gene48249.t1 MTR_8g042900 48.980 441 197 13 24 440 145 581 4.62e-127 380
MS.gene48249.t1 MTR_4g087970 45.475 442 207 8 26 440 83 517 4.37e-126 375
MS.gene48249.t1 MTR_4g124520 48.515 404 187 10 53 440 123 521 3.84e-122 365
MS.gene48249.t1 MTR_0458s0030 64.639 263 89 3 181 440 53 314 2.00e-121 356
MS.gene48249.t1 MTR_1g088040 64.639 263 89 3 181 440 53 314 2.00e-121 356
MS.gene48249.t1 MTR_2g044810 44.766 449 221 11 12 440 93 534 3.47e-120 361
MS.gene48249.t1 MTR_4g124550 43.478 460 221 10 17 440 137 593 2.82e-119 360
MS.gene48249.t1 MTR_3g010770 55.589 331 135 6 121 440 246 575 3.59e-118 357
MS.gene48249.t1 MTR_1g086210 40.812 468 228 10 21 440 103 569 1.75e-113 345
MS.gene48249.t1 MTR_0618s0020 66.520 227 73 2 217 440 2 228 3.36e-111 327
MS.gene48249.t1 MTR_1g083510 39.959 483 239 13 7 439 101 582 7.70e-104 320
MS.gene48249.t1 MTR_8g023150 44.581 406 204 8 55 440 178 582 8.07e-103 318
MS.gene48249.t1 MTR_1g086190 40.132 456 232 12 24 440 110 563 3.64e-102 315
MS.gene48249.t1 MTR_7g110490 39.700 466 225 10 24 435 106 569 2.02e-100 311
MS.gene48249.t1 MTR_7g110510 39.700 466 225 10 24 435 106 569 2.02e-100 311
MS.gene48249.t1 MTR_7g110560 40.308 454 226 13 24 440 104 549 2.96e-100 310
MS.gene48249.t1 MTR_3g095920 41.333 450 218 14 30 439 120 563 4.32e-99 307
MS.gene48249.t1 MTR_2g044660 47.605 334 166 7 111 440 304 632 7.65e-99 309
MS.gene48249.t1 MTR_1g083470 38.147 464 227 13 19 436 117 566 3.10e-98 306
MS.gene48249.t1 MTR_1g083500 38.147 464 227 13 19 436 117 566 3.10e-98 306
MS.gene48249.t1 MTR_7g110440 37.792 471 237 10 24 440 106 574 8.11e-98 305
MS.gene48249.t1 MTR_8g093460 40.826 436 216 12 30 429 111 540 3.05e-97 303
MS.gene48249.t1 MTR_7g112590 37.988 487 252 14 1 439 98 582 1.78e-96 301
MS.gene48249.t1 MTR_4g005170 38.106 454 241 13 19 436 122 571 1.38e-95 300
MS.gene48249.t1 MTR_2g044810 55.378 251 108 3 193 440 216 465 1.20e-94 293
MS.gene48249.t1 MTR_1g083490 37.528 453 245 12 19 436 122 571 1.71e-94 296
MS.gene48249.t1 MTR_4g005180 36.638 464 234 13 19 436 104 553 1.33e-91 288
MS.gene48249.t1 MTR_4g120290 37.418 457 241 13 24 439 119 571 1.27e-89 283
MS.gene48249.t1 MTR_4g120280 36.980 457 243 12 24 439 119 571 2.73e-88 280
MS.gene48249.t1 MTR_2g436050 38.000 450 243 14 24 439 116 563 3.76e-87 276
MS.gene48249.t1 MTR_2g436040 35.374 441 256 10 24 437 105 543 3.96e-87 278
MS.gene48249.t1 MTR_8g088610 43.917 337 169 10 113 437 45 373 1.06e-84 264
MS.gene48249.t1 MTR_7g110420 34.979 486 242 12 24 440 106 586 9.96e-82 263
MS.gene48249.t1 MTR_0458s0010 38.525 488 199 18 10 440 75 518 1.02e-79 256
MS.gene48249.t1 MTR_5g083620 40.762 341 189 8 110 440 19 356 8.56e-79 248
MS.gene48249.t1 MTR_7g110430 33.333 468 228 9 24 440 106 540 8.62e-79 254
MS.gene48249.t1 MTR_7g107180 41.139 316 170 7 131 440 2 307 1.69e-72 231
MS.gene48249.t1 MTR_2g105560 40.705 312 167 7 135 440 40 339 1.87e-71 229
MS.gene48249.t1 MTR_7g111560 31.788 453 271 14 17 440 113 556 8.11e-67 223
MS.gene48249.t1 MTR_2g039530 40.594 303 162 8 110 406 28 318 8.65e-58 193
MS.gene48249.t1 MTR_6g024165 37.072 321 176 10 121 429 28 334 1.34e-52 180
MS.gene48249.t1 MTR_6g088810 39.735 302 152 9 144 429 90 377 9.16e-52 179
MS.gene48249.t1 MTR_6g012800 35.644 303 167 9 136 423 6 295 5.69e-50 172
MS.gene48249.t1 MTR_8g102380 38.065 310 162 10 137 429 81 377 9.33e-50 173
MS.gene48249.t1 MTR_6g042660 34.545 275 157 7 136 402 37 296 1.16e-45 161
MS.gene48249.t1 MTR_7g011530 34.091 308 176 8 138 429 8 304 1.29e-45 160
MS.gene48249.t1 MTR_5g083590 31.529 314 158 7 134 440 5 268 9.21e-45 157
MS.gene48249.t1 MTR_1g086540 32.919 322 189 8 121 429 37 344 1.76e-44 159
MS.gene48249.t1 MTR_7g092780 31.548 336 198 8 108 429 70 387 5.54e-44 158
MS.gene48249.t1 MTR_2g033970 31.746 315 192 8 125 429 45 346 9.30e-41 149
MS.gene48249.t1 MTR_8g102380 38.976 254 128 8 137 373 81 324 1.89e-40 147
MS.gene48249.t1 MTR_6g042690 32.979 282 161 7 134 402 37 303 1.97e-39 145
MS.gene48249.t1 MTR_1g033050 30.892 314 186 8 131 433 13 306 2.47e-39 144
MS.gene48249.t1 MTR_5g095490 33.225 307 170 8 142 429 67 357 4.80e-38 142
MS.gene48249.t1 MTR_5g093970 32.787 305 182 9 136 432 43 332 2.70e-37 139
MS.gene48249.t1 MTR_4g124740 31.366 322 184 10 124 429 39 339 5.54e-36 135
MS.gene48249.t1 MTR_5g088300 30.537 298 189 6 136 431 37 318 7.47e-35 132
MS.gene48249.t1 MTR_2g436600 31.803 305 174 11 136 429 55 336 2.30e-34 131
MS.gene48249.t1 MTR_7g102600 31.169 308 185 7 138 431 101 395 3.13e-34 132
MS.gene48249.t1 MTR_5g012600 29.808 312 192 8 136 432 63 362 2.06e-33 129
MS.gene48249.t1 MTR_5g088280 29.195 298 193 6 136 431 39 320 1.61e-31 123
MS.gene48249.t1 MTR_4g074290 29.063 320 193 11 125 429 61 361 1.92e-31 124
MS.gene48249.t1 MTR_8g036225 27.541 305 195 6 136 429 33 322 3.32e-31 122
MS.gene48249.t1 MTR_5g088290 29.605 304 185 9 136 431 48 330 4.67e-31 122
MS.gene48249.t1 MTR_2g073390 26.975 367 230 13 81 429 15 361 7.78e-31 122
MS.gene48249.t1 MTR_4g051810 30.233 301 187 8 136 431 37 319 1.39e-30 120
MS.gene48249.t1 MTR_0009s0350 28.981 314 201 9 125 429 61 361 1.48e-29 119
MS.gene48249.t1 MTR_0009s0340 28.981 314 201 9 125 429 61 361 1.48e-29 119
MS.gene48249.t1 MTR_6g025590 36.158 177 100 5 121 286 36 210 2.39e-22 95.9
MS.gene48249.t1 MTR_0408s0030 37.895 95 52 1 308 402 36 123 1.47e-16 77.0
MS.gene48249.t1 MTR_2g436070 30.000 230 132 9 2 204 94 321 2.40e-15 77.8
MS.gene48249.t1 MTR_6g024200 34.591 159 83 7 273 429 137 276 3.03e-14 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48249.t1 AT2G45220 70.562 445 122 6 1 440 71 511 0.0 642
MS.gene48249.t1 AT5G51490 53.219 466 188 10 1 440 75 536 5.03e-165 475
MS.gene48249.t1 AT5G51500 52.371 464 195 9 1 440 79 540 6.03e-157 455
MS.gene48249.t1 AT4G00190 51.339 448 195 7 1 440 42 474 8.84e-154 444
MS.gene48249.t1 AT3G47400 50.858 466 203 9 1 440 129 594 2.63e-153 447
MS.gene48249.t1 AT1G23200 50.000 460 179 8 25 440 102 554 6.87e-150 437
MS.gene48249.t1 AT3G59010 51.389 432 183 5 29 440 104 528 5.58e-142 416
MS.gene48249.t1 AT1G11580 51.136 440 194 9 22 440 118 557 9.75e-142 417
MS.gene48249.t1 AT1G11580 51.136 440 194 9 22 440 139 578 2.28e-141 416
MS.gene48249.t1 AT5G53370 48.337 451 202 7 21 440 135 585 1.78e-140 414
MS.gene48249.t1 AT3G49220 48.889 450 199 9 21 440 148 596 4.77e-140 414
MS.gene48249.t1 AT5G53370 48.337 451 202 7 21 440 171 621 5.54e-140 414
MS.gene48249.t1 AT3G49220 48.889 450 199 9 21 440 193 641 1.77e-139 414
MS.gene48249.t1 AT3G14310 48.421 475 189 11 20 440 120 592 2.19e-138 409
MS.gene48249.t1 AT4G02320 47.661 449 206 10 20 440 71 518 1.17e-136 402
MS.gene48249.t1 AT3G43270 48.107 449 196 9 26 440 80 525 1.66e-136 402
MS.gene48249.t1 AT1G53830 48.718 468 190 11 20 440 123 587 2.44e-136 404
MS.gene48249.t1 AT3G05620 47.034 472 207 11 2 440 80 541 7.95e-136 401
MS.gene48249.t1 AT3G60730 45.833 456 206 8 24 440 66 519 1.82e-133 394
MS.gene48249.t1 AT2G43050 49.296 426 193 9 29 440 100 516 5.12e-131 388
MS.gene48249.t1 AT4G33220 45.887 462 197 9 26 440 68 523 7.91e-131 388
MS.gene48249.t1 AT4G02300 45.598 443 216 8 23 440 90 532 1.82e-128 382
MS.gene48249.t1 AT2G47550 49.784 462 183 13 26 440 99 558 4.24e-128 382
MS.gene48249.t1 AT4G02330 47.964 442 172 12 53 440 134 571 8.95e-128 382
MS.gene48249.t1 AT1G02810 48.552 449 162 13 55 440 135 577 2.33e-125 375
MS.gene48249.t1 AT4G33220 54.491 334 144 5 113 440 71 402 2.49e-124 367
MS.gene48249.t1 AT2G26450 44.592 453 222 13 14 440 162 611 2.41e-119 361
MS.gene48249.t1 AT5G20860 44.444 432 214 9 30 440 112 538 2.12e-118 356
MS.gene48249.t1 AT5G04960 44.037 436 219 9 25 438 130 562 3.60e-118 357
MS.gene48249.t1 AT3G10710 45.977 435 202 10 30 440 136 561 4.78e-116 351
MS.gene48249.t1 AT5G49180 46.247 413 186 11 55 436 159 566 2.42e-114 347
MS.gene48249.t1 AT3G05610 41.060 453 234 12 19 440 115 565 3.81e-114 349
MS.gene48249.t1 AT3G10720 43.750 480 212 13 16 440 139 615 5.81e-114 347
MS.gene48249.t1 AT2G26440 42.889 450 222 10 23 440 101 547 1.95e-113 344
MS.gene48249.t1 AT4G33230 43.103 464 226 12 12 440 146 606 6.84e-112 342
MS.gene48249.t1 AT5G04970 42.827 481 215 13 16 440 144 620 1.56e-110 339
MS.gene48249.t1 AT5G27870 39.468 451 242 11 19 440 114 562 3.80e-110 341
MS.gene48249.t1 AT1G11590 43.373 415 224 7 37 440 110 524 2.88e-107 327
MS.gene48249.t1 AT3G06830 39.741 463 230 13 19 438 110 566 5.11e-107 328
MS.gene48249.t1 AT4G03930 43.133 415 225 7 37 440 110 524 6.98e-107 326
MS.gene48249.t1 AT3G27980 43.284 402 202 6 55 440 106 497 2.28e-106 324
MS.gene48249.t1 AT3G10720 57.692 260 106 3 184 440 1 259 2.95e-104 310
MS.gene48249.t1 AT1G53840 40.948 464 223 13 14 434 126 581 6.75e-103 318
MS.gene48249.t1 AT4G15980 47.278 349 174 7 99 438 353 700 6.20e-102 319
MS.gene48249.t1 AT4G15980 47.278 349 174 7 99 438 363 710 1.46e-101 318
MS.gene48249.t1 AT3G14300 41.441 444 214 11 24 429 523 958 2.63e-99 318
MS.gene48249.t1 AT5G64640 39.865 444 222 13 30 433 164 602 3.34e-95 298
MS.gene48249.t1 AT5G09760 40.367 436 218 13 30 428 116 546 8.33e-93 291
MS.gene48249.t1 AT5G09760 40.367 436 218 13 30 428 142 572 1.70e-92 291
MS.gene48249.t1 AT5G20860 40.955 398 182 8 30 406 112 477 2.18e-92 288
MS.gene48249.t1 AT2G47030 43.678 348 175 8 112 440 243 588 7.11e-83 266
MS.gene48249.t1 AT2G47040 43.768 345 176 8 112 440 253 595 1.74e-80 260
MS.gene48249.t1 AT3G62170 41.292 356 180 8 112 440 235 588 8.73e-79 255
MS.gene48249.t1 AT1G11370 49.187 246 119 4 133 372 43 288 1.17e-74 235
MS.gene48249.t1 AT5G19730 37.134 307 167 8 137 429 87 381 2.07e-48 170
MS.gene48249.t1 AT3G29090 35.789 285 162 5 131 402 2 278 1.41e-47 166
MS.gene48249.t1 AT1G05310 33.038 339 195 9 105 429 68 388 4.45e-44 159
MS.gene48249.t1 AT3G17060 31.861 317 189 8 125 429 35 336 5.21e-44 157
MS.gene48249.t1 AT2G36700 32.667 300 184 5 138 429 44 333 1.33e-43 156
MS.gene48249.t1 AT2G36710 33.019 318 184 11 133 434 86 390 1.59e-40 149
MS.gene48249.t1 AT1G44980 43.617 188 101 3 114 296 54 241 1.63e-40 145
MS.gene48249.t1 AT2G21610 30.100 299 187 7 136 429 51 332 2.28e-38 142
MS.gene48249.t1 AT2G21610 30.100 299 187 7 136 429 56 337 2.45e-38 142
MS.gene48249.t1 AT5G18990 34.035 285 166 6 131 402 24 299 3.60e-38 141
MS.gene48249.t1 AT5G55590 33.333 309 179 9 136 429 82 378 2.55e-37 140
MS.gene48249.t1 AT2G19150 31.579 323 191 10 128 436 33 339 1.41e-36 137
MS.gene48249.t1 AT3G24130 32.155 283 170 6 133 402 31 304 7.39e-36 135
MS.gene48249.t1 AT5G47500 28.267 329 198 8 116 429 52 357 1.40e-35 135
MS.gene48249.t1 AT5G07430 29.067 375 229 14 71 429 2 355 2.38e-35 134
MS.gene48249.t1 AT5G26810 30.793 328 197 10 122 431 20 335 5.92e-35 133
MS.gene48249.t1 AT5G55590 35.573 253 139 8 136 373 82 325 1.65e-34 131
MS.gene48249.t1 AT3G49220 35.616 219 113 7 21 212 193 410 5.78e-31 124
MS.gene48249.t1 AT2G47280 30.421 309 184 8 136 429 35 327 9.16e-31 121
MS.gene48249.t1 AT2G19150 32.171 258 152 8 128 373 33 279 1.54e-29 117
MS.gene48249.t1 AT5G07420 27.859 341 214 10 107 429 29 355 6.55e-29 117
MS.gene48249.t1 AT5G61680 27.885 312 200 9 129 429 61 358 2.56e-26 109
MS.gene48249.t1 AT5G07410 26.163 344 227 10 99 429 26 355 2.86e-26 109
MS.gene48249.t1 AT1G69940 25.946 370 244 12 75 429 1 355 1.74e-25 107

Find 96 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACAACTTTGTTACATTAT+TGG 0.127213 4.2:+77411741 None:intergenic
GGGACAGTTGATTTCATCTT+TGG 0.221019 4.2:-77409506 MS.gene48249:CDS
GAATTATAATTCATAATTCT+AGG 0.221921 4.2:-77409376 MS.gene48249:CDS
TACAGAGAATGTAACATCTA+TGG 0.256911 4.2:-77409527 MS.gene48249:CDS
CAGGTTCATCCACTTCAAAT+AGG 0.323866 4.2:-77409163 MS.gene48249:CDS
TTCATGAAAACTGTTATTGA+TGG 0.343065 4.2:-77409272 MS.gene48249:CDS
AAGCTGTACTAAGCCATGTT+TGG 0.344214 4.2:+77411836 None:intergenic
CAAGCTGTTGCATTTCGTTC+CGG 0.356258 4.2:-77409629 MS.gene48249:CDS
ACCGTTGGGAATTTCATTGC+CGG 0.366873 4.2:-77409086 MS.gene48249:CDS
GAATATGCTAATACAGGTTC+AGG 0.371500 4.2:-77409182 MS.gene48249:CDS
AGCTGTACTAAGCCATGTTT+GGG 0.379379 4.2:+77411837 None:intergenic
ATTTCATTGCCGGTAATTCA+TGG 0.379520 4.2:-77409076 MS.gene48249:CDS
CATCTTTGTAACTTGGTTGT+TGG 0.388751 4.2:+77411686 None:intergenic
GTTTCCTATCCATGAATTAC+CGG 0.395370 4.2:+77409067 None:intergenic
TTGTAACTTGGTTGTTGGTA+AGG 0.414505 4.2:+77411691 None:intergenic
AGGTTCATCCACTTCAAATA+GGG 0.421888 4.2:-77409162 MS.gene48249:CDS
TTTGCAGCTGCTACTGGTGA+TGG 0.425142 4.2:-77409704 MS.gene48249:intron
AAGCTTCACCTTTCACCGTT+GGG 0.443952 4.2:-77409100 MS.gene48249:CDS
CAAGCTTCACCTTTCACCGT+TGG 0.453573 4.2:-77409101 MS.gene48249:CDS
TCGTTCCGGCTCCGATCTAT+CGG 0.457003 4.2:-77409615 MS.gene48249:CDS
CCACTTCAAATAGGGTTACA+TGG 0.460645 4.2:-77409154 MS.gene48249:CDS
TATGCTGAATATGCTAATAC+AGG 0.469023 4.2:-77409188 MS.gene48249:CDS
CATGGGTTAAACCTGGTGAT+AGG 0.470207 4.2:-77411654 MS.gene48249:CDS
ATGAAATTCCCAACGGTGAA+AGG 0.479388 4.2:+77409092 None:intergenic
CAACAACCAAGTTACAAAGA+TGG 0.480845 4.2:-77411685 MS.gene48249:CDS
CCCTGCTGGTTGGTTACCAT+GGG 0.482029 4.2:-77409240 MS.gene48249:CDS
TCCTTGCGCGGTAACTGTGA+TGG 0.482943 4.2:+77409424 None:intergenic
AATTATAATTCATAATTCTA+GGG 0.485667 4.2:-77409375 MS.gene48249:CDS
ACAGGCGTGCCATTCACCTC+TGG 0.485673 4.2:-77409044 MS.gene48249:CDS
CTGTTAAATCGTACCTTGGT+AGG 0.486089 4.2:-77409322 MS.gene48249:CDS
CCATGTAACCCTATTTGAAG+TGG 0.490411 4.2:+77409154 None:intergenic
GGAAATCCATCTTTGTAACT+TGG 0.499245 4.2:+77411679 None:intergenic
ATTGCCGGTAATTCATGGAT+AGG 0.500552 4.2:-77409071 MS.gene48249:CDS
TCCCATGGTAACCAACCAGC+AGG 0.502238 4.2:+77409239 None:intergenic
GTCGTGGCTAAAGATGGTTC+TGG 0.502309 4.2:-77411589 MS.gene48249:CDS
ACAGAGAATGTAACATCTAT+GGG 0.505050 4.2:-77409526 MS.gene48249:CDS
ACCCTGCTGGTTGGTTACCA+TGG 0.509749 4.2:-77409241 MS.gene48249:CDS
TCTTGTGGGCTACGGCACTT+AGG 0.510740 4.2:+77411952 None:intergenic
AACATCTTCTGCTGCTTCGA+AGG 0.513839 4.2:-77411620 MS.gene48249:CDS
GGTACGATTTAACAGAGCTA+GGG 0.515662 4.2:+77409330 None:intergenic
ATGTTGGTGGGTGATGGTAT+TGG 0.525394 4.2:-77411448 MS.gene48249:CDS
TGAAGAAGCTTCCTATCACC+AGG 0.526790 4.2:+77411643 None:intergenic
TCAAATAGGGTTACATGGAA+AGG 0.533882 4.2:-77409149 MS.gene48249:CDS
AGGTACGATTTAACAGAGCT+AGG 0.534156 4.2:+77409329 None:intergenic
ATTGGTAAAACCATAATCAC+TGG 0.538242 4.2:-77411430 MS.gene48249:CDS
TCACCAGGTTTAACCCATGT+TGG 0.540325 4.2:+77411658 None:intergenic
GATGCTGCACCTAGTGGTAG+TGG 0.541082 4.2:-77411538 MS.gene48249:CDS
ATCACTGGTAGCAAAAGTGT+TGG 0.542034 4.2:-77411415 MS.gene48249:CDS
ACCATCACAGTTACCGCGCA+AGG 0.543990 4.2:-77409425 MS.gene48249:CDS
AATGTCGTCGTGGCTAAAGA+TGG 0.547500 4.2:-77411595 MS.gene48249:CDS
ACAAAGATGGATTTCCAACA+TGG 0.557740 4.2:-77411672 MS.gene48249:CDS
AATGTCATGTTGGTGGGTGA+TGG 0.561648 4.2:-77411454 MS.gene48249:CDS
ACTGGTAGCAAAAGTGTTGG+AGG 0.563435 4.2:-77411412 MS.gene48249:CDS
ATCACATTTAGAAACACAGC+TGG 0.566335 4.2:-77409665 MS.gene48249:CDS
TAAAGCAAAGAATGTCATGT+TGG 0.567010 4.2:-77411464 MS.gene48249:CDS
TTTGTAAAAGACCGATAGAT+CGG 0.567687 4.2:+77409604 None:intergenic
GGTTGCTGAACGGAAGGTAG+TGG 0.568060 4.2:+77411384 None:intergenic
ACCGATCCAAATCAAAACAC+TGG 0.569193 4.2:-77409398 MS.gene48249:CDS
AATCGTACCTTGGTAGGCCG+TGG 0.576253 4.2:-77409316 MS.gene48249:CDS
AAAGACCGATAGATCGGAGC+CGG 0.577312 4.2:+77409610 None:intergenic
GTTACATTATTGGACAACAA+AGG 0.577990 4.2:+77411751 None:intergenic
CACAATATGAATCCCAAACA+TGG 0.578929 4.2:-77411849 MS.gene48249:CDS
GCTTAAAGTCCAGAGGTGAA+TGG 0.579277 4.2:+77409035 None:intergenic
GAAGAAAATACACATGCACT+TGG 0.581338 4.2:-77411976 MS.gene48249:CDS
ACCTTCCGTTCAGCAACCGT+TGG 0.584686 4.2:-77411379 MS.gene48249:intron
TTTCCAACATGGGTTAAACC+TGG 0.589586 4.2:-77411661 MS.gene48249:CDS
CGGAACGAAATGCAACAGCT+TGG 0.595802 4.2:+77409630 None:intergenic
AGCAAAGAATGTCATGTTGG+TGG 0.597389 4.2:-77411461 MS.gene48249:CDS
TATGTGATATATGTGAAGGC+TGG 0.599695 4.2:-77411511 MS.gene48249:CDS
TAACACTTTGTCTCTTAACA+AGG 0.603266 4.2:-77411716 MS.gene48249:CDS
GCTGGTTGGTTACCATGGGA+TGG 0.604546 4.2:-77409236 MS.gene48249:CDS
TGGCAAGTACACGACGGTGA+AGG 0.606204 4.2:-77411569 MS.gene48249:CDS
AAAGCAAAATTACCATCCCA+TGG 0.612268 4.2:+77409224 None:intergenic
CACGACGGTGAAGGCCGCGA+CGG 0.612289 4.2:-77411560 MS.gene48249:CDS
AGCTCTGTTAAATCGTACCT+TGG 0.625217 4.2:-77409326 MS.gene48249:CDS
ACCAACGGTTGCTGAACGGA+AGG 0.627356 4.2:+77411378 None:intergenic
CAAAGATGGATTTCCAACAT+GGG 0.627787 4.2:-77411671 MS.gene48249:CDS
CTGCACCTAGTGGTAGTGGA+AGG 0.630490 4.2:-77411534 MS.gene48249:CDS
GCAAAGAATGTCATGTTGGT+GGG 0.630792 4.2:-77411460 MS.gene48249:CDS
GCTTACCAACGGTTGCTGAA+CGG 0.634972 4.2:+77411374 None:intergenic
AAGAAAATACACATGCACTT+GGG 0.638213 4.2:-77411975 MS.gene48249:CDS
GGTAGCAAAAGTGTTGGAGG+TGG 0.640198 4.2:-77411409 MS.gene48249:CDS
GAGAAGCACTAGTAAGAACA+TGG 0.644219 4.2:+77409123 None:intergenic
TGGATCGGTTCTTCCTTGCG+CGG 0.648642 4.2:+77409412 None:intergenic
GCGACGGATGCTGCACCTAG+TGG 0.650197 4.2:-77411544 MS.gene48249:CDS
AAGGTATGTGATATATGTGA+AGG 0.653633 4.2:-77411515 MS.gene48249:CDS
ACATACCTTCCACTACCACT+AGG 0.655775 4.2:+77411529 None:intergenic
TTCGAAGGCTAATGTCGTCG+TGG 0.658493 4.2:-77411605 MS.gene48249:CDS
CCCATGGTAACCAACCAGCA+GGG 0.670836 4.2:+77409240 None:intergenic
ACCGGCAATGAAATTCCCAA+CGG 0.671958 4.2:+77409085 None:intergenic
AAGAAACGCTTAAAGTCCAG+AGG 0.675031 4.2:+77409028 None:intergenic
AATTCATGGATAGGAAACAC+AGG 0.687418 4.2:-77409062 MS.gene48249:CDS
ACTAGGTGCAGCATCCGTCG+CGG 0.690402 4.2:+77411546 None:intergenic
AGACATAGTTTGTGACACCA+AGG 0.696122 4.2:+77411776 None:intergenic
TGGTTCTGGCAAGTACACGA+CGG 0.752914 4.2:-77411575 MS.gene48249:CDS
TTTCATCTTTGGAAACGCCG+CGG 0.774990 4.2:-77409495 MS.gene48249:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAAATATTTAAATTAA+TGG + chr4.2:77409986-77410005 None:intergenic 0.0%
!! AAATAAATAATTATATCAGT+AGG - chr4.2:77410253-77410272 MS.gene48249:intron 10.0%
!! AAATATAACAAATAACTTAA+AGG + chr4.2:77410135-77410154 None:intergenic 10.0%
!! AATAAATAATTATATCAGTA+GGG - chr4.2:77410254-77410273 MS.gene48249:intron 10.0%
!! AATTATAATTCATAATTCTA+GGG - chr4.2:77411659-77411678 MS.gene48249:CDS 10.0%
!! ATTTCTTAATCATTAAATTA+AGG + chr4.2:77410607-77410626 None:intergenic 10.0%
!! TTTCTTAATCATTAAATTAA+GGG + chr4.2:77410606-77410625 None:intergenic 10.0%
!! AAAATTAATTTAACTCTAGA+TGG + chr4.2:77410072-77410091 None:intergenic 15.0%
!! ACTTTAACTAATTTCTTAAT+TGG - chr4.2:77410978-77410997 MS.gene48249:intron 15.0%
!! ATAAATAATTATATCAGTAG+GGG - chr4.2:77410255-77410274 MS.gene48249:intron 15.0%
!! GAATTATAATTCATAATTCT+AGG - chr4.2:77411658-77411677 MS.gene48249:CDS 15.0%
!! TTAAGGAAAAATATTAATCT+AGG + chr4.2:77410926-77410945 None:intergenic 15.0%
!! TTCAATTAAATCAATTATTG+TGG + chr4.2:77410638-77410657 None:intergenic 15.0%
!! TTTAATTAAAAGAAGAATGT+GGG - chr4.2:77411076-77411095 MS.gene48249:intron 15.0%
!!! AGATTAATATTTTTCCTTAA+TGG - chr4.2:77410926-77410945 MS.gene48249:intron 15.0%
!!! ATTACTTTAAAATCTCTTTA+CGG - chr4.2:77410516-77410535 MS.gene48249:intron 15.0%
!!! ATTCAATTCATTATGTTTTT+GGG + chr4.2:77410674-77410693 None:intergenic 15.0%
!!! ATTTACAAAAAAAAAAGTGT+TGG - chr4.2:77410096-77410115 MS.gene48249:intron 15.0%
!!! TATTCAATTCATTATGTTTT+TGG + chr4.2:77410675-77410694 None:intergenic 15.0%
!! AATCTTGAAACATGTAAAAA+TGG - chr4.2:77409226-77409245 MS.gene48249:CDS 20.0%
!! CTAGTTTATGATTTAGTTAT+TGG + chr4.2:77409832-77409851 None:intergenic 20.0%
!! CTTTAATTAAAAGAAGAATG+TGG - chr4.2:77411075-77411094 MS.gene48249:intron 20.0%
!! GAAAATTACTTATGAATGAT+AGG - chr4.2:77410021-77410040 MS.gene48249:intron 20.0%
!! GAATAAGACTATATTAAAGT+GGG + chr4.2:77411045-77411064 None:intergenic 20.0%
!! GAATAGTTATTTATATCCTT+TGG + chr4.2:77410472-77410491 None:intergenic 20.0%
!! TAACAACTTTGTTACATTAT+TGG + chr4.2:77409296-77409315 None:intergenic 20.0%
!! TATGATTTGAATGATTTACA+TGG - chr4.2:77410733-77410752 MS.gene48249:intron 20.0%
!! TGAATAAGACTATATTAAAG+TGG + chr4.2:77411046-77411065 None:intergenic 20.0%
!! TTAATTAAAAGAAGAATGTG+GGG - chr4.2:77411077-77411096 MS.gene48249:intron 20.0%
!! TTAGGATAATATATCATAGA+AGG + chr4.2:77410543-77410562 None:intergenic 20.0%
!! TTCAACTGATTATTAACTTA+TGG + chr4.2:77411120-77411139 None:intergenic 20.0%
!!! AGATTTTAAAGTAATCACAA+TGG + chr4.2:77410511-77410530 None:intergenic 20.0%
!!! ATCATAGTCTAAAACTTTAT+TGG + chr4.2:77410719-77410738 None:intergenic 20.0%
!!! ATTTGTGAACTTTTCTTTAA+GGG + chr4.2:77409725-77409744 None:intergenic 20.0%
!!! CACATTCTTCTTTTAATTAA+AGG + chr4.2:77411077-77411096 None:intergenic 20.0%
!!! GCTTTTATATAATTAGGATT+AGG + chr4.2:77410561-77410580 None:intergenic 20.0%
!!! TATTTGTGAACTTTTCTTTA+AGG + chr4.2:77409726-77409745 None:intergenic 20.0%
!!! TGCATAAGATTTTTCAATAA+AGG + chr4.2:77409862-77409881 None:intergenic 20.0%
! ACATAATGAATTGAATATCG+TGG - chr4.2:77410678-77410697 MS.gene48249:intron 25.0%
! ACTTATGAATGATAGGTAAA+CGG - chr4.2:77410028-77410047 MS.gene48249:intron 25.0%
! ATTATCTACTTTCTTACTCA+AGG + chr4.2:77410446-77410465 None:intergenic 25.0%
! TTAAGGGATAAAAAAAGCTA+CGG + chr4.2:77409709-77409728 None:intergenic 25.0%
! TTCATGAAAACTGTTATTGA+TGG - chr4.2:77411762-77411781 MS.gene48249:CDS 25.0%
!! CAGGTAAGAACTTTTTAATA+AGG + chr4.2:77409771-77409790 None:intergenic 25.0%
!! TGAATGAACTTTTCTTTTCT+TGG + chr4.2:77409800-77409819 None:intergenic 25.0%
!!! AAAAATGGATTTTATGACCT+TGG - chr4.2:77409241-77409260 MS.gene48249:CDS 25.0%
!!! ATAATTCCAGTGTTTTGATT+TGG + chr4.2:77411645-77411664 None:intergenic 25.0%
!!! CTGCTTGCTTTTATATAATT+AGG + chr4.2:77410567-77410586 None:intergenic 25.0%
!!! TTTTACAAATGTAGCTTTGA+AGG - chr4.2:77411444-77411463 MS.gene48249:CDS 25.0%
AAGAAAATACACATGCACTT+GGG - chr4.2:77409059-77409078 MS.gene48249:CDS 30.0%
AAGGTATGTGATATATGTGA+AGG - chr4.2:77409519-77409538 MS.gene48249:CDS 30.0%
ACAGAGAATGTAACATCTAT+GGG - chr4.2:77411508-77411527 MS.gene48249:CDS 30.0%
ATTGGTAAAACCATAATCAC+TGG - chr4.2:77409604-77409623 MS.gene48249:CDS 30.0%
CACATAGGAAACTATATATG+CGG + chr4.2:77410953-77410972 None:intergenic 30.0%
CTAGGATGATTTATTCTAGA+GGG + chr4.2:77410908-77410927 None:intergenic 30.0%
CTTATGAATGATAGGTAAAC+GGG - chr4.2:77410029-77410048 MS.gene48249:intron 30.0%
CTTTAGATTTGTGTGGTATA+TGG - chr4.2:77410382-77410401 MS.gene48249:intron 30.0%
GAAAGTAGATAATAGACCAA+AGG - chr4.2:77410453-77410472 MS.gene48249:intron 30.0%
GGCTTTCTATATGTTATAGT+AGG + chr4.2:77410876-77410895 None:intergenic 30.0%
GTATTGTCTTACATTTAGCA+AGG - chr4.2:77410799-77410818 MS.gene48249:intron 30.0%
GTTACATTATTGGACAACAA+AGG + chr4.2:77409286-77409305 None:intergenic 30.0%
TAAAGCAAAGAATGTCATGT+TGG - chr4.2:77409570-77409589 MS.gene48249:CDS 30.0%
TAACACTTTGTCTCTTAACA+AGG - chr4.2:77409318-77409337 MS.gene48249:CDS 30.0%
TACAGAGAATGTAACATCTA+TGG - chr4.2:77411507-77411526 MS.gene48249:CDS 30.0%
TATGCTGAATATGCTAATAC+AGG - chr4.2:77411846-77411865 MS.gene48249:CDS 30.0%
TGAGAAGCTATGTATAACAA+CGG - chr4.2:77410299-77410318 MS.gene48249:intron 30.0%
TGCTTAGAAAGTTAACTAGT+CGG - chr4.2:77409889-77409908 MS.gene48249:intron 30.0%
TGTGTAGCTTTCAAAAATGA+AGG - chr4.2:77410769-77410788 MS.gene48249:intron 30.0%
TTATGAATGATAGGTAAACG+GGG - chr4.2:77410030-77410049 MS.gene48249:intron 30.0%
TTTGTAAAAGACCGATAGAT+CGG + chr4.2:77411433-77411452 None:intergenic 30.0%
! TAGGATGATTTATTCTAGAG+GGG + chr4.2:77410907-77410926 None:intergenic 30.0%
! TCTAGGATGATTTATTCTAG+AGG + chr4.2:77410909-77410928 None:intergenic 30.0%
! TGCACTTTGTGTATTTTGTT+TGG + chr4.2:77409165-77409184 None:intergenic 30.0%
!! GTATTGACTTTAGATTTGTG+TGG - chr4.2:77410375-77410394 MS.gene48249:intron 30.0%
!! GTTCTTGAATATTTTTGCCA+CGG + chr4.2:77411738-77411757 None:intergenic 30.0%
AAAGCAAAATTACCATCCCA+TGG + chr4.2:77411813-77411832 None:intergenic 35.0%
AAGATATGCAACCTTAACAC+CGG + chr4.2:77410335-77410354 None:intergenic 35.0%
AATTCATGGATAGGAAACAC+AGG - chr4.2:77411972-77411991 MS.gene48249:CDS 35.0%
ACTATATATGCGGTCCATTA+AGG + chr4.2:77410943-77410962 None:intergenic 35.0%
AGGTTCATCCACTTCAAATA+GGG - chr4.2:77411872-77411891 MS.gene48249:CDS 35.0%
ATCACATTTAGAAACACAGC+TGG - chr4.2:77411369-77411388 MS.gene48249:intron 35.0%
ATTTCATTGCCGGTAATTCA+TGG - chr4.2:77411958-77411977 MS.gene48249:CDS 35.0%
CAACAACCAAGTTACAAAGA+TGG - chr4.2:77409349-77409368 MS.gene48249:CDS 35.0%
CACAATATGAATCCCAAACA+TGG - chr4.2:77409185-77409204 MS.gene48249:CDS 35.0%
CATCTTTGTAACTTGGTTGT+TGG + chr4.2:77409351-77409370 None:intergenic 35.0%
CGCATATATAGTTTCCTATG+TGG - chr4.2:77410951-77410970 MS.gene48249:intron 35.0%
GAAGAAAATACACATGCACT+TGG - chr4.2:77409058-77409077 MS.gene48249:CDS 35.0%
GAATATGCTAATACAGGTTC+AGG - chr4.2:77411852-77411871 MS.gene48249:CDS 35.0%
GAGTATTAAAAAACCTAGCG+TGG + chr4.2:77411298-77411317 None:intergenic 35.0%
GGAAATCCATCTTTGTAACT+TGG + chr4.2:77409358-77409377 None:intergenic 35.0%
GTTTCCTATCCATGAATTAC+CGG + chr4.2:77411970-77411989 None:intergenic 35.0%
TACATAGCTTCTCATTCTAG+AGG + chr4.2:77410293-77410312 None:intergenic 35.0%
TATGTGATATATGTGAAGGC+TGG - chr4.2:77409523-77409542 MS.gene48249:CDS 35.0%
TATTAAAGTGGGTCCTACAA+TGG + chr4.2:77411034-77411053 None:intergenic 35.0%
TCAAATAGGGTTACATGGAA+AGG - chr4.2:77411885-77411904 MS.gene48249:CDS 35.0%
TTGTAACTTGGTTGTTGGTA+AGG + chr4.2:77409346-77409365 None:intergenic 35.0%
TTTGTGTGGTATATGGCAAT+TGG - chr4.2:77410389-77410408 MS.gene48249:intron 35.0%
! ACAAAGATGGATTTCCAACA+TGG - chr4.2:77409362-77409381 MS.gene48249:CDS 35.0%
! ACAATTTTGCAAAACTACCG+CGG + chr4.2:77411559-77411578 None:intergenic 35.0%
! ACTTTTGCTACCAGTGATTA+TGG + chr4.2:77409617-77409636 None:intergenic 35.0%
! ATGGGATGGTAATTTTGCTT+TGG - chr4.2:77411812-77411831 MS.gene48249:CDS 35.0%
! CAAAGATGGATTTCCAACAT+GGG - chr4.2:77409363-77409382 MS.gene48249:CDS 35.0%
! TTACTTGAAGTGCTTACCAA+CGG + chr4.2:77409674-77409693 None:intergenic 35.0%
! TTTCTTTTCTTGGTCAAGAC+AGG + chr4.2:77409790-77409809 None:intergenic 35.0%
! TTTGGATTGGAATCTGCAAT+TGG - chr4.2:77411175-77411194 MS.gene48249:intron 35.0%
!! TAGTTAAAGTGTTGCCACAT+AGG + chr4.2:77410968-77410987 None:intergenic 35.0%
!!! TCCAGTGTTTTGATTTGGAT+CGG + chr4.2:77411640-77411659 None:intergenic 35.0%
!!! TGTTTTTGGGCTCAATTATG+TGG + chr4.2:77410661-77410680 None:intergenic 35.0%
!!! TTTTTTTTTGCAGCTGCTAC+TGG - chr4.2:77411324-77411343 MS.gene48249:intron 35.0%
AAGCTGTACTAAGCCATGTT+TGG + chr4.2:77409201-77409220 None:intergenic 40.0%
ACCGATCCAAATCAAAACAC+TGG - chr4.2:77411636-77411655 MS.gene48249:CDS 40.0%
AGCAAAGAATGTCATGTTGG+TGG - chr4.2:77409573-77409592 MS.gene48249:CDS 40.0%
AGCTCTGTTAAATCGTACCT+TGG - chr4.2:77411708-77411727 MS.gene48249:CDS 40.0%
AGCTGTACTAAGCCATGTTT+GGG + chr4.2:77409200-77409219 None:intergenic 40.0%
AGGTACGATTTAACAGAGCT+AGG + chr4.2:77411708-77411727 None:intergenic 40.0%
ATGAAATTCCCAACGGTGAA+AGG + chr4.2:77411945-77411964 None:intergenic 40.0%
ATTGCCGGTAATTCATGGAT+AGG - chr4.2:77411963-77411982 MS.gene48249:CDS 40.0%
CACAATTCCACATCCATTGT+AGG - chr4.2:77411018-77411037 MS.gene48249:intron 40.0%
CAGGTTCATCCACTTCAAAT+AGG - chr4.2:77411871-77411890 MS.gene48249:CDS 40.0%
CCACTTCAAATAGGGTTACA+TGG - chr4.2:77411880-77411899 MS.gene48249:CDS 40.0%
CCATGTAACCCTATTTGAAG+TGG + chr4.2:77411883-77411902 None:intergenic 40.0%
CTGTTAAATCGTACCTTGGT+AGG - chr4.2:77411712-77411731 MS.gene48249:CDS 40.0%
GCAAAGAATGTCATGTTGGT+GGG - chr4.2:77409574-77409593 MS.gene48249:CDS 40.0%
GGTACGATTTAACAGAGCTA+GGG + chr4.2:77411707-77411726 None:intergenic 40.0%
TGTATAACAACGGTCCTTCT+CGG - chr4.2:77410309-77410328 MS.gene48249:intron 40.0%
! ACACGTCATGCATTTTGGAT+TGG - chr4.2:77411162-77411181 MS.gene48249:intron 40.0%
! AGACATAGTTTGTGACACCA+AGG + chr4.2:77409261-77409280 None:intergenic 40.0%
! AGTACACACGTCATGCATTT+TGG - chr4.2:77411157-77411176 MS.gene48249:intron 40.0%
! ATCACTGGTAGCAAAAGTGT+TGG - chr4.2:77409619-77409638 MS.gene48249:CDS 40.0%
! GGGACAGTTGATTTCATCTT+TGG - chr4.2:77411528-77411547 MS.gene48249:CDS 40.0%
! GTAACTGTGATGGTTTTTGC+AGG + chr4.2:77411603-77411622 None:intergenic 40.0%
! TATTCTAGAGGGGAAAGATG+TGG + chr4.2:77410897-77410916 None:intergenic 40.0%
! TTTCCAACATGGGTTAAACC+TGG - chr4.2:77409373-77409392 MS.gene48249:CDS 40.0%
!! GAGAAGCACTAGTAAGAACA+TGG + chr4.2:77411914-77411933 None:intergenic 40.0%
!! GTTTTATTAACCCTGCTGGT+TGG - chr4.2:77411784-77411803 MS.gene48249:CDS 40.0%
!!! GATGGTTTTATTAACCCTGC+TGG - chr4.2:77411780-77411799 MS.gene48249:CDS 40.0%
AAGCTTCACCTTTCACCGTT+GGG - chr4.2:77411934-77411953 MS.gene48249:CDS 45.0%
AATGTCGTCGTGGCTAAAGA+TGG - chr4.2:77409439-77409458 MS.gene48249:CDS 45.0%
ACATACCTTCCACTACCACT+AGG + chr4.2:77409508-77409527 None:intergenic 45.0%
ACCGGCAATGAAATTCCCAA+CGG + chr4.2:77411952-77411971 None:intergenic 45.0%
ACCGTTGGGAATTTCATTGC+CGG - chr4.2:77411948-77411967 MS.gene48249:CDS 45.0%
CAAGCTGTTGCATTTCGTTC+CGG - chr4.2:77411405-77411424 MS.gene48249:CDS 45.0%
CCCACAAGAAAAAGCTGCTT+GGG - chr4.2:77409096-77409115 MS.gene48249:CDS 45.0%
TCACCAGGTTTAACCCATGT+TGG + chr4.2:77409379-77409398 None:intergenic 45.0%
TGAAGAAGCTTCCTATCACC+AGG + chr4.2:77409394-77409413 None:intergenic 45.0%
! AACATCTTCTGCTGCTTCGA+AGG - chr4.2:77409414-77409433 MS.gene48249:CDS 45.0%
! AATGTCATGTTGGTGGGTGA+TGG - chr4.2:77409580-77409599 MS.gene48249:CDS 45.0%
! ACTGTGATGGTTTTTGCAGG+CGG + chr4.2:77411600-77411619 None:intergenic 45.0%
! ATGTTGGTGGGTGATGGTAT+TGG - chr4.2:77409586-77409605 MS.gene48249:CDS 45.0%
! CATGGGTTAAACCTGGTGAT+AGG - chr4.2:77409380-77409399 MS.gene48249:CDS 45.0%
! TTTCATCTTTGGAAACGCCG+CGG - chr4.2:77411539-77411558 MS.gene48249:CDS 45.0%
!! ACTGGTAGCAAAAGTGTTGG+AGG - chr4.2:77409622-77409641 MS.gene48249:CDS 45.0%
!! CAGCTTTTTCTTGTGGGCTA+CGG + chr4.2:77409093-77409112 None:intergenic 45.0%
!! CCCAAGCAGCTTTTTCTTGT+GGG + chr4.2:77409099-77409118 None:intergenic 45.0%
AAAGACCGATAGATCGGAGC+CGG + chr4.2:77411427-77411446 None:intergenic 50.0%
AATTGCTTGTGCTCCACGCT+AGG - chr4.2:77411282-77411301 MS.gene48249:intron 50.0%
AGTGGGTCCTACAATGGATG+TGG + chr4.2:77411028-77411047 None:intergenic 50.0%
CAAGCTTCACCTTTCACCGT+TGG - chr4.2:77411933-77411952 MS.gene48249:CDS 50.0%
CGGAACGAAATGCAACAGCT+TGG + chr4.2:77411407-77411426 None:intergenic 50.0%
GCCCACAAGAAAAAGCTGCT+TGG - chr4.2:77409095-77409114 MS.gene48249:CDS 50.0%
GCTTACCAACGGTTGCTGAA+CGG + chr4.2:77409663-77409682 None:intergenic 50.0%
GTCGTGGCTAAAGATGGTTC+TGG - chr4.2:77409445-77409464 MS.gene48249:CDS 50.0%
TTCGAAGGCTAATGTCGTCG+TGG - chr4.2:77409429-77409448 MS.gene48249:CDS 50.0%
TTTGCAGCTGCTACTGGTGA+TGG - chr4.2:77411330-77411349 MS.gene48249:intron 50.0%
! ATTTTTGCCACGGCCTACCA+AGG + chr4.2:77411728-77411747 None:intergenic 50.0%
! CTGTGATGGTTTTTGCAGGC+GGG + chr4.2:77411599-77411618 None:intergenic 50.0%
!! GCCCAAGCAGCTTTTTCTTG+TGG + chr4.2:77409100-77409119 None:intergenic 50.0%
!! GGTAGCAAAAGTGTTGGAGG+TGG - chr4.2:77409625-77409644 MS.gene48249:CDS 50.0%
!! TGGTTCTGGCAAGTACACGA+CGG - chr4.2:77409459-77409478 MS.gene48249:CDS 50.0%
AACCTTAACACCGGCCGAGA+AGG + chr4.2:77410326-77410345 None:intergenic 55.0%
AATCGTACCTTGGTAGGCCG+TGG - chr4.2:77411718-77411737 MS.gene48249:CDS 55.0%
ACCAACGGTTGCTGAACGGA+AGG + chr4.2:77409659-77409678 None:intergenic 55.0%
ACCATCACAGTTACCGCGCA+AGG - chr4.2:77411609-77411628 MS.gene48249:CDS 55.0%
ACCTTCCGTTCAGCAACCGT+TGG - chr4.2:77409655-77409674 MS.gene48249:CDS 55.0%
CCCATGGTAACCAACCAGCA+GGG + chr4.2:77411797-77411816 None:intergenic 55.0%
GATGCTGCACCTAGTGGTAG+TGG - chr4.2:77409496-77409515 MS.gene48249:CDS 55.0%
GGTTGCTGAACGGAAGGTAG+TGG + chr4.2:77409653-77409672 None:intergenic 55.0%
TAACAACGGTCCTTCTCGGC+CGG - chr4.2:77410313-77410332 MS.gene48249:intron 55.0%
TCCCATGGTAACCAACCAGC+AGG + chr4.2:77411798-77411817 None:intergenic 55.0%
TCCTTGCGCGGTAACTGTGA+TGG + chr4.2:77411613-77411632 None:intergenic 55.0%
TCGTTCCGGCTCCGATCTAT+CGG - chr4.2:77411419-77411438 MS.gene48249:CDS 55.0%
TGGCAAGTACACGACGGTGA+AGG - chr4.2:77409465-77409484 MS.gene48249:CDS 55.0%
! ACCCTGCTGGTTGGTTACCA+TGG - chr4.2:77411793-77411812 MS.gene48249:CDS 55.0%
! CCCTGCTGGTTGGTTACCAT+GGG - chr4.2:77411794-77411813 MS.gene48249:CDS 55.0%
! GCTGGTTGGTTACCATGGGA+TGG - chr4.2:77411798-77411817 MS.gene48249:CDS 55.0%
! TCTTGTGGGCTACGGCACTT+AGG + chr4.2:77409085-77409104 None:intergenic 55.0%
! TGGATCGGTTCTTCCTTGCG+CGG + chr4.2:77411625-77411644 None:intergenic 55.0%
!! CTGCACCTAGTGGTAGTGGA+AGG - chr4.2:77409500-77409519 MS.gene48249:CDS 55.0%
ACTAGGTGCAGCATCCGTCG+CGG + chr4.2:77409491-77409510 None:intergenic 60.0%
! ACAGGCGTGCCATTCACCTC+TGG - chr4.2:77411990-77412009 MS.gene48249:CDS 60.0%
! GTCCTTCTCGGCCGGTGTTA+AGG - chr4.2:77410321-77410340 MS.gene48249:intron 60.0%
GCGACGGATGCTGCACCTAG+TGG - chr4.2:77409490-77409509 MS.gene48249:CDS 65.0%
CACGACGGTGAAGGCCGCGA+CGG - chr4.2:77409474-77409493 MS.gene48249:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 77409037 77412019 77409037 ID=MS.gene48249
chr4.2 mRNA 77409037 77412019 77409037 ID=MS.gene48249.t1;Parent=MS.gene48249
chr4.2 exon 77411380 77412019 77411380 ID=MS.gene48249.t1.exon1;Parent=MS.gene48249.t1
chr4.2 CDS 77411380 77412019 77411380 ID=cds.MS.gene48249.t1;Parent=MS.gene48249.t1
chr4.2 exon 77409037 77409719 77409037 ID=MS.gene48249.t1.exon2;Parent=MS.gene48249.t1
chr4.2 CDS 77409037 77409719 77409037 ID=cds.MS.gene48249.t1;Parent=MS.gene48249.t1
Gene Sequence

>MS.gene48249

ATGGAGAGAGCAAAAAAGGGTGAAGAAAATACACATGCACTTGGGCCTAAGTGCCGTAGCCCACAAGAAAAAGCTGCTTGGGCAGATTGTCTTGAACTCTATGATTTCACTGTTCAAAAGCTTAGCCAAACAAAATACACAAAGTGCACACAATATGAATCCCAAACATGGCTTAGTACAGCTTTAACAAATCTTGAAACATGTAAAAATGGATTTTATGACCTTGGTGTCACAAACTATGTCTTACCTTTGTTGTCCAATAATGTAACAAAGTTGTTAAGTAACACTTTGTCTCTTAACAAGGTTCCTTACCAACAACCAAGTTACAAAGATGGATTTCCAACATGGGTTAAACCTGGTGATAGGAAGCTTCTTCAAACATCTTCTGCTGCTTCGAAGGCTAATGTCGTCGTGGCTAAAGATGGTTCTGGCAAGTACACGACGGTGAAGGCCGCGACGGATGCTGCACCTAGTGGTAGTGGAAGGTATGTGATATATGTGAAGGCTGGTGTGTATAATGAACAAGTTGAGATTAAAGCAAAGAATGTCATGTTGGTGGGTGATGGTATTGGTAAAACCATAATCACTGGTAGCAAAAGTGTTGGAGGTGGCACCACTACCTTCCGTTCAGCAACCGTTGGTAAGCACTTCAAGTAACACTTTATTTTACCGTAGCTTTTTTTATCCCTTAAAGAAAAGTTCACAAATAAGACTATCATTATGAATTATTACCTTATTAAAAAGTTCTTACCTGTCTTGACCAAGAAAAGAAAAGTTCATTCATTTTATCAACCAATAACTAAATCATAAACTAGTCACTTTCCTTTATTGAAAAATCTTATGCAAATTTCTTGCTTAGAAAGTTAACTAGTCGGAATAATTCAAAGCTATAAAAAGAATACATATGCTTTTTTCTACTCATGAATAAATATGCGATATGTTTCAACCATTAATTTAAATATTTATTTATTTATTTATATACATGAAAATTACTTATGAATGATAGGTAAACGGGGTATACTTATTTTATTTCCATCTAGAGTTAAATTAATTTTTAATATTTACAAAAAAAAAAGTGTTGGTGATTATCTCTAACCTTTAAGTTATTTGTTATATTTATACTATTAGAATTTTATTATAATTTCATAATCAATAAAAATAAATATACATACTTGTTACTATTATAATCTACATATATTTTCGTTTTATGAAAAATAAATAAATAATTATATCAGTAGGGGTATAGAAAATAACCTCTAGAATGAGAAGCTATGTATAACAACGGTCCTTCTCGGCCGGTGTTAAGGTTGCATATCTTATTGAACTTGAATAATATTCGTATTGACTTTAGATTTGTGTGGTATATGGCAATTGGCAAATTATCAATTACTCATAGACAAATGAGTCCTTGAGTAAGAAAGTAGATAATAGACCAAAGGATATAAATAACTATTCAAAATGATACATTATGCCATTGTGATTACTTTAAAATCTCTTTACGGTCCTTCTATGATATATTATCCTAATCCTAATTATATAAAAGCAAGCAGCAAAGTTGAATTATGTCCCTTAATTTAATGATTAAGAAATTTATTAGACCACAATAATTGATTTAATTGAACCACATAATTGAGCCCAAAAACATAATGAATTGAATATCGTGGATTGAAATTTACAATCCAATAAAGTTTTAGACTATGATTTGAATGATTTACATGGATGATTGAATTATTGTGTAGCTTTCAAAAATGAAGGAGCACATGTATTGTCTTACATTTAGCAAGGACAAAGATCAAAATAAGATTTTTAGTAGTCAAATCTCTAGAAGAAGACGTGCCTACTATAACATATAGAAAGCCACATCTTTCCCCTCTAGAATAAATCATCCTAGATTAATATTTTTCCTTAATGGACCGCATATATAGTTTCCTATGTGGCAACACTTTAACTAATTTCTTAATTGGTAGCTCAAACTTGTTTTCACAATTCCACATCCATTGTAGGACCCACTTTAATATAGTCTTATTCAATTAAGATCCTTTAATTAAAAGAAGAATGTGGGGCTTTAGAAGCATGTATACCATAAGTTAATAATCAGTTGAACTATATAAATTAATCATAGTACACACGTCATGCATTTTGGATTGGAATCTGCAATTGGTTAGAATGTCACTTTGAGATTTGAAAAATGTACACATAAACTAATAGTCAATGCAACTGTTTTTTATTACTTATTTTTCATTTCAATTGCTTGTGCTCCACGCTAGGTTTTTTAATACTCTTTTTTTTTTTTTTTGCAGCTGCTACTGGTGATGGATTTATTGCTCAAGACATCACATTTAGAAACACAGCTGGAGCTGCAAATCACCAAGCTGTTGCATTTCGTTCCGGCTCCGATCTATCGGTCTTTTACAAATGTAGCTTTGAAGGTTATCAAGACACACTTTATGTTCACTCAGAGAGACAATTTTACAGAGAATGTAACATCTATGGGACAGTTGATTTCATCTTTGGAAACGCCGCGGTAGTTTTGCAAAATTGTAACATTTTTGCAAGAAACCCGCCTGCAAAAACCATCACAGTTACCGCGCAAGGAAGAACCGATCCAAATCAAAACACTGGAATTATAATTCATAATTCTAGGGTTTCTGCTCAATCAGATTTAAACCCTAGCTCTGTTAAATCGTACCTTGGTAGGCCGTGGCAAAAATATTCAAGAACAGTTTTCATGAAAACTGTTATTGATGGTTTTATTAACCCTGCTGGTTGGTTACCATGGGATGGTAATTTTGCTTTGGATACTTTATATTATGCTGAATATGCTAATACAGGTTCAGGTTCATCCACTTCAAATAGGGTTACATGGAAAGGTTACCATGTTCTTACTAGTGCTTCTCAAGCTTCACCTTTCACCGTTGGGAATTTCATTGCCGGTAATTCATGGATAGGAAACACAGGCGTGCCATTCACCTCTGGACTTTAA
Protein sequence

>MS.gene48249.t1

MERAKKGEENTHALGPKCRSPQEKAAWADCLELYDFTVQKLSQTKYTKCTQYESQTWLSTALTNLETCKNGFYDLGVTNYVLPLLSNNVTKLLSNTLSLNKVPYQQPSYKDGFPTWVKPGDRKLLQTSSAASKANVVVAKDGSGKYTTVKAATDAAPSGSGRYVIYVKAGVYNEQVEIKAKNVMLVGDGIGKTIITGSKSVGGGTTTFRSATVAATGDGFIAQDITFRNTAGAANHQAVAFRSGSDLSVFYKCSFEGYQDTLYVHSERQFYRECNIYGTVDFIFGNAAVVLQNCNIFARNPPAKTITVTAQGRTDPNQNTGIIIHNSRVSAQSDLNPSSVKSYLGRPWQKYSRTVFMKTVIDGFINPAGWLPWDGNFALDTLYYAEYANTGSGSSTSNRVTWKGYHVLTSASQASPFTVGNFIAGNSWIGNTGVPFTSGL