AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49973

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49973.t1 MTR_1g105495 99.333 300 2 0 1 300 1 300 0.0 627
MS.gene49973.t1 MTR_4g097700 51.439 278 131 2 17 294 51 324 4.47e-101 299
MS.gene49973.t1 MTR_4g097640 53.435 262 118 2 33 294 30 287 1.41e-99 294
MS.gene49973.t1 MTR_4g097670 53.435 262 118 2 33 294 30 287 1.41e-99 294
MS.gene49973.t1 MTR_1g105900 45.918 294 154 3 5 296 3 293 1.73e-97 289
MS.gene49973.t1 MTR_7g084770 48.966 290 144 2 5 294 2 287 1.40e-94 281
MS.gene49973.t1 MTR_6g033055 47.751 289 147 2 7 295 4 288 1.34e-93 279
MS.gene49973.t1 MTR_7g084760 48.454 291 146 2 5 295 2 288 1.85e-93 279
MS.gene49973.t1 MTR_6g033085 48.592 284 142 2 12 295 9 288 1.90e-93 278
MS.gene49973.t1 MTR_7g084750 48.227 282 142 2 13 294 10 287 7.90e-93 277
MS.gene49973.t1 MTR_2g089140 49.265 272 126 4 30 294 20 286 1.74e-91 273
MS.gene49973.t1 MTR_4g045853 45.946 296 138 5 6 294 8 288 1.88e-88 266
MS.gene49973.t1 MTR_4g128410 46.780 295 142 5 5 297 2 283 4.59e-87 262
MS.gene49973.t1 MTR_7g093530 47.079 291 141 5 8 296 2 281 4.05e-86 259
MS.gene49973.t1 MTR_4g128570 46.528 288 131 5 11 297 10 275 4.41e-84 254
MS.gene49973.t1 MTR_8g081040 44.792 288 143 6 9 295 10 282 1.06e-83 253
MS.gene49973.t1 MTR_3g084620 43.929 280 145 3 16 294 9 277 9.34e-83 251
MS.gene49973.t1 MTR_3g084630 44.245 278 143 3 18 294 11 277 1.10e-82 251
MS.gene49973.t1 MTR_8g479340 64.179 201 12 2 100 300 1 141 4.05e-82 244
MS.gene49973.t1 MTR_4g128580 46.931 277 133 5 28 297 19 288 8.74e-81 246
MS.gene49973.t1 MTR_4g126920 45.055 273 139 4 29 297 19 284 1.74e-80 245
MS.gene49973.t1 MTR_2g089140 52.000 225 98 3 77 294 2 223 7.22e-80 242
MS.gene49973.t1 MTR_8g013055 46.360 261 133 4 35 294 31 285 1.21e-79 243
MS.gene49973.t1 MTR_2g038860 43.986 291 149 4 11 297 9 289 2.03e-79 243
MS.gene49973.t1 MTR_2g038705 43.986 291 149 4 11 297 9 289 2.03e-79 243
MS.gene49973.t1 MTR_6g088320 41.958 286 152 5 12 294 9 283 1.35e-78 241
MS.gene49973.t1 MTR_8g102220 43.060 281 136 5 18 297 12 269 1.76e-77 237
MS.gene49973.t1 MTR_4g128400 42.807 285 151 4 14 294 7 283 4.80e-77 237
MS.gene49973.t1 MTR_8g102910 40.767 287 157 5 11 294 3 279 3.37e-76 234
MS.gene49973.t1 MTR_4g128590 41.414 297 149 5 5 297 3 278 6.93e-74 228
MS.gene49973.t1 MTR_7g056090 40.956 293 161 7 4 296 3 283 5.14e-72 224
MS.gene49973.t1 MTR_5g091730 41.065 263 144 4 33 294 26 278 4.30e-68 214
MS.gene49973.t1 MTR_3g089540 42.803 264 140 5 33 294 28 282 1.91e-65 207
MS.gene49973.t1 MTR_2g049130 36.861 274 164 7 9 281 3 268 2.78e-61 196
MS.gene49973.t1 MTR_2g049030 35.052 291 172 8 10 298 5 280 5.69e-59 190
MS.gene49973.t1 MTR_4g057450 37.993 279 156 8 21 294 38 304 1.44e-58 190
MS.gene49973.t1 MTR_8g064180 35.099 302 179 7 8 294 11 310 2.22e-58 189
MS.gene49973.t1 MTR_5g029100 38.686 274 156 6 35 300 42 311 2.48e-58 191
MS.gene49973.t1 MTR_2g095800 37.979 287 156 9 16 294 37 309 7.08e-57 186
MS.gene49973.t1 MTR_1g063020 34.768 302 165 9 9 294 9 294 1.54e-55 182
MS.gene49973.t1 MTR_8g090100 35.316 269 169 4 35 300 21 287 2.35e-54 179
MS.gene49973.t1 MTR_7g102260 37.132 272 144 8 35 295 37 292 9.36e-54 177
MS.gene49973.t1 MTR_1g033420 36.704 267 158 6 35 296 33 293 1.69e-53 177
MS.gene49973.t1 MTR_5g029100 39.419 241 134 6 68 300 44 280 1.66e-52 174
MS.gene49973.t1 MTR_8g032640 34.682 173 106 4 59 231 35 200 5.83e-18 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49973.t1 AT1G11545 82.877 292 45 3 10 299 16 304 0.0 515
MS.gene49973.t1 AT5G13870 50.859 291 138 3 4 294 2 287 5.21e-98 290
MS.gene49973.t1 AT5G13870 50.859 291 138 3 4 294 2 287 5.21e-98 290
MS.gene49973.t1 AT5G13870 50.859 291 138 3 4 294 2 287 5.21e-98 290
MS.gene49973.t1 AT4G37800 49.141 291 141 4 6 295 6 290 2.86e-97 288
MS.gene49973.t1 AT5G65730 47.735 287 146 2 8 294 8 290 3.35e-96 286
MS.gene49973.t1 AT2G06850 53.846 260 116 2 35 294 35 290 2.78e-93 278
MS.gene49973.t1 AT2G06850 57.143 231 95 2 65 295 5 231 3.15e-90 268
MS.gene49973.t1 AT2G14620 45.675 289 151 3 11 296 11 296 4.32e-90 270
MS.gene49973.t1 AT4G03210 46.528 288 142 5 9 294 7 284 1.95e-89 268
MS.gene49973.t1 AT5G57560 44.369 293 142 5 8 294 4 281 4.99e-86 259
MS.gene49973.t1 AT3G23730 43.813 299 152 5 4 297 2 289 2.05e-84 256
MS.gene49973.t1 AT5G57550 46.102 295 145 7 1 294 1 282 1.29e-83 253
MS.gene49973.t1 AT5G57540 48.881 268 127 5 33 297 24 284 1.34e-83 253
MS.gene49973.t1 AT5G57530 47.794 272 132 5 29 297 21 285 3.67e-83 252
MS.gene49973.t1 AT4G25810 43.310 284 157 2 12 294 3 283 7.44e-83 251
MS.gene49973.t1 AT4G03210 52.174 230 104 3 67 294 31 256 1.32e-82 250
MS.gene49973.t1 AT4G30270 43.003 293 141 5 6 297 1 268 2.84e-81 247
MS.gene49973.t1 AT4G25820 46.520 273 134 4 28 296 23 287 4.48e-80 244
MS.gene49973.t1 AT4G14130 42.905 296 157 6 4 297 2 287 2.14e-79 243
MS.gene49973.t1 AT2G14620 49.138 232 114 2 66 296 18 246 4.91e-79 240
MS.gene49973.t1 AT4G13090 42.756 283 155 4 15 294 10 288 1.18e-77 238
MS.gene49973.t1 AT2G14620 49.776 223 108 2 75 296 16 235 2.30e-76 233
MS.gene49973.t1 AT5G48070 41.297 293 157 6 5 295 3 282 1.25e-75 233
MS.gene49973.t1 AT3G25050 38.966 290 169 5 6 294 7 289 3.46e-75 232
MS.gene49973.t1 AT4G13080 40.138 289 166 4 6 294 10 291 7.70e-74 229
MS.gene49973.t1 AT4G28850 41.053 285 159 5 15 295 9 288 7.83e-74 229
MS.gene49973.t1 AT2G18800 39.423 312 157 7 1 295 1 297 5.69e-72 224
MS.gene49973.t1 AT4G30280 40.678 295 155 6 3 295 6 282 1.70e-70 220
MS.gene49973.t1 AT4G30290 39.932 293 156 6 5 295 3 277 3.01e-68 214
MS.gene49973.t1 AT1G65310 43.019 265 140 5 33 295 27 282 3.43e-68 214
MS.gene49973.t1 AT1G65310 43.019 265 140 5 33 295 49 304 3.86e-68 214
MS.gene49973.t1 AT2G01850 35.714 294 181 4 11 300 7 296 2.33e-61 198
MS.gene49973.t1 AT1G32170 38.079 302 177 6 5 300 2 299 4.05e-61 197
MS.gene49973.t1 AT3G44990 37.874 301 157 7 12 294 5 293 1.60e-60 194
MS.gene49973.t1 AT1G14720 35.836 293 180 4 12 300 8 296 5.81e-59 192
MS.gene49973.t1 AT1G10550 35.740 277 160 4 35 294 34 309 7.96e-57 186
MS.gene49973.t1 AT4G18990 35.714 280 165 3 35 300 40 318 5.51e-55 182
MS.gene49973.t1 AT2G36870 34.000 300 176 8 6 294 9 297 7.48e-53 175
MS.gene49973.t1 AT3G48580 33.977 259 149 8 37 294 36 273 4.67e-48 162
MS.gene49973.t1 AT3G48580 36.364 231 126 7 65 294 4 214 1.71e-46 156
MS.gene49973.t1 AT4G18990 35.021 237 133 5 84 300 9 244 7.43e-41 143
MS.gene49973.t1 AT3G48580 28.571 259 143 9 37 294 36 253 1.78e-31 118
MS.gene49973.t1 AT2G36870 35.260 173 98 4 6 167 9 178 4.26e-28 107

Find 66 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCAAGTTCATCTCTTTC+TGG 0.152227 1.1:-75445205 None:intergenic
TATGATGCTTGGCATCTTTC+AGG 0.170390 1.1:+75446292 MS.gene49973:CDS
TTGTTCTTAAACACCCTTAT+TGG 0.217303 1.1:-75446049 None:intergenic
AAGTACATTGGTTTCTCATT+TGG 0.290156 1.1:-75446094 None:intergenic
ACATTTGTTTGAATCAAATA+TGG 0.300118 1.1:-75445256 None:intergenic
TTGTGGATAGAGTTCCAATA+AGG 0.315911 1.1:+75446035 MS.gene49973:CDS
TCTTTCAGGTGATCAGAAAA+TGG 0.325557 1.1:+75446306 MS.gene49973:CDS
AAGAATGAACTTACACTATC+TGG 0.346292 1.1:-75445375 None:intergenic
GTTCAGTATGAAGCTGAAAT+TGG 0.347997 1.1:+75444987 MS.gene49973:CDS
AGTACATTGGTTTCTCATTT+GGG 0.354048 1.1:-75446093 None:intergenic
AGCAGAAGCAGCTATGTCTA+AGG 0.356338 1.1:+75444378 MS.gene49973:CDS
GTTGAAACACATTTAGGAAA+TGG 0.376286 1.1:-75446244 None:intergenic
ATGATAATAAGCAGCTACTA+TGG 0.378445 1.1:-75444353 None:intergenic
ATGTCTCATCTCACGATTAC+CGG 0.385651 1.1:-75445293 None:intergenic
TCATTTGGGAAGAAATTGTT+TGG 0.386974 1.1:-75446079 None:intergenic
CATCTGTAGAGGTAGTAAAA+TGG 0.396419 1.1:-75444441 None:intergenic
GTTGTGGTTGAAACACATTT+AGG 0.398748 1.1:-75446250 None:intergenic
ACAAAACAAAGATACAGATT+TGG 0.405420 1.1:+75444961 MS.gene49973:CDS
ATCACACCTATTCTATTCTC+TGG 0.408329 1.1:+75445344 MS.gene49973:CDS
ATCAGAAAATGGATTATGCT+TGG 0.410696 1.1:+75446317 MS.gene49973:CDS
CAAACAAATGTGTACAAGAA+TGG 0.414124 1.1:+75445268 MS.gene49973:CDS
TTGCAGATGTGTACAGAAAA+TGG 0.418568 1.1:+75445175 MS.gene49973:intron
ATCAACACTAAAGTCTTTGT+AGG 0.426921 1.1:-75446203 None:intergenic
GTGAGATGAGACATATGCTT+TGG 0.427321 1.1:+75445302 MS.gene49973:CDS
AAACAAATGTGTACAAGAAT+GGG 0.444792 1.1:+75445269 MS.gene49973:CDS
AAGAATGGAAAATGAAGAAA+AGG 0.445067 1.1:-75444317 None:intergenic
TGTGGATAGAGTTCCAATAA+GGG 0.449235 1.1:+75446036 MS.gene49973:CDS
GAGCACTCCTCAGGCAAAGT+TGG 0.451574 1.1:-75446391 None:intergenic
TCAGAAAATGGATTATGCTT+GGG 0.479320 1.1:+75446318 MS.gene49973:CDS
AGGAGTGCTCATTGAGTCCA+TGG 0.481061 1.1:+75446404 MS.gene49973:CDS
GTTATTCCAGAGAATAGAAT+AGG 0.498751 1.1:-75445350 None:intergenic
GGCTGCAAGGAAAAGAAGAA+TGG 0.506963 1.1:-75444332 None:intergenic
GTGTATGGAATGCAGATGAT+TGG 0.516261 1.1:+75446125 MS.gene49973:CDS
ATGTGTACAGAAAATGGTGC+AGG 0.516508 1.1:+75445181 MS.gene49973:CDS
GGACTCAATGAGCACTCCTC+AGG 0.517455 1.1:-75446400 None:intergenic
TTAGTGTTGATGCTTGTCAA+TGG 0.533968 1.1:+75446215 MS.gene49973:CDS
ACACTTGAAAACAAGTACAT+TGG 0.541308 1.1:-75446106 None:intergenic
CCTCTACAGATGGACAGATC+TGG 0.542190 1.1:+75444452 MS.gene49973:CDS
TAGTGTTGATGCTTGTCAAT+GGG 0.548485 1.1:+75446216 MS.gene49973:CDS
TGTATGGAATGCAGATGATT+GGG 0.552558 1.1:+75446126 MS.gene49973:CDS
CCCATGATACATTACTCCCA+TGG 0.558590 1.1:-75446421 None:intergenic
GATGATTGGGCCACAAGAGG+TGG 0.559220 1.1:+75446139 MS.gene49973:CDS
TCCTACCCAAAATGGATGGA+GGG 0.570885 1.1:+75444293 None:intergenic
GCAGAAGCAGCTATGTCTAA+GGG 0.571565 1.1:+75444379 MS.gene49973:CDS
CTCCTACCCAAAATGGATGG+AGG 0.581100 1.1:+75444292 None:intergenic
CTCCTCCTACCCAAAATGGA+TGG 0.582425 1.1:+75444289 None:intergenic
TGCAATAGTCATAGATGACA+AGG 0.582934 1.1:-75446351 None:intergenic
TTGGTAGGAGGTGATTCTGC+TGG 0.585894 1.1:+75445006 MS.gene49973:CDS
AACAAAGATACAGATTTGGA+TGG 0.590115 1.1:+75444965 MS.gene49973:CDS
GGTGGGATCAATATGATGCT+TGG 0.593619 1.1:+75446281 MS.gene49973:CDS
AGTATGAAGCTGAAATTGGT+AGG 0.601753 1.1:+75444991 MS.gene49973:CDS
GCAGATGATTGGGCCACAAG+AGG 0.605501 1.1:+75446136 MS.gene49973:CDS
AATGTGTACAAGAATGGGAC+CGG 0.607915 1.1:+75445274 MS.gene49973:CDS
AAGCAGCTACTATGGCTGCA+AGG 0.616062 1.1:-75444345 None:intergenic
CCAGATCTGTCCATCTGTAG+AGG 0.619087 1.1:-75444452 None:intergenic
TAGGTGTGATAGTCCTCAGT+GGG 0.619254 1.1:-75445331 None:intergenic
AAGACAACTTCAGCATAATG+TGG 0.628622 1.1:+75444410 MS.gene49973:CDS
TCTTTGTAGGATGACACAAA+TGG 0.631516 1.1:-75446190 None:intergenic
TTGAATGTTTGACAGGATGT+GGG 0.642879 1.1:+75444932 MS.gene49973:intron
GGAGTGCTCATTGAGTCCAT+GGG 0.646187 1.1:+75446405 MS.gene49973:CDS
ATGAAGCTGAAATTGGTAGG+AGG 0.646766 1.1:+75444994 MS.gene49973:CDS
TGTGTACAGAAAATGGTGCA+GGG 0.655933 1.1:+75445182 MS.gene49973:CDS
AATGAACTTACACTATCTGG+TGG 0.659740 1.1:-75445372 None:intergenic
CTCTCATTAGACAATGACAC+AGG 0.668040 1.1:+75444478 MS.gene49973:CDS
GCTTTGGTTTGATCCCACTG+AGG 0.724888 1.1:+75445318 MS.gene49973:CDS
ATAGGTGTGATAGTCCTCAG+TGG 0.744401 1.1:-75445332 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGCATTTAATTTTCAAT+GGG + chr1.1:75445704-75445723 MS.gene49973:intron 15.0%
!!! TAAATGCATTTAATTTTCAA+TGG + chr1.1:75445703-75445722 MS.gene49973:intron 15.0%
!!! TTTTCGATAGAATTAATTTT+GGG + chr1.1:75444784-75444803 MS.gene49973:intron 15.0%
!! AAAAAGACCTTATATTTCAA+CGG + chr1.1:75445923-75445942 MS.gene49973:intron 20.0%
!! ACATTTGTTTGAATCAAATA+TGG - chr1.1:75445259-75445278 None:intergenic 20.0%
!! ACCTTAATTCAAGATATTTA+TGG + chr1.1:75445963-75445982 MS.gene49973:intron 20.0%
!! AGTAAAATGTATGTACAATA+TGG + chr1.1:75445814-75445833 MS.gene49973:intron 20.0%
!! ATGAATATGATTATGTCATA+TGG + chr1.1:75445872-75445891 MS.gene49973:intron 20.0%
!! TCCATAAATATCTTGAATTA+AGG - chr1.1:75445967-75445986 None:intergenic 20.0%
!!! AAAGTTGAGAGTATAATTTA+TGG + chr1.1:75444822-75444841 MS.gene49973:intron 20.0%
!!! AACATTCAATGCTTTAAAAA+TGG - chr1.1:75444921-75444940 None:intergenic 20.0%
!!! AGTTTAAGACTTTTTGTTTT+TGG + chr1.1:75445550-75445569 MS.gene49973:intron 20.0%
!!! CTTTTCGATAGAATTAATTT+TGG + chr1.1:75444783-75444802 MS.gene49973:intron 20.0%
!!! GATTTACATAAACTTACTTT+TGG + chr1.1:75444717-75444736 MS.gene49973:intron 20.0%
!!! TTGATGTCATTTTTTTACTT+GGG - chr1.1:75445434-75445453 None:intergenic 20.0%
!!! TTTGATGTCATTTTTTTACT+TGG - chr1.1:75445435-75445454 None:intergenic 20.0%
! AAAATGTATGTACAATATGG+TGG + chr1.1:75445817-75445836 MS.gene49973:intron 25.0%
! AAACAAATGTGTACAAGAAT+GGG + chr1.1:75445269-75445288 MS.gene49973:CDS 25.0%
! AAATGTATGTACAATATGGT+GGG + chr1.1:75445818-75445837 MS.gene49973:intron 25.0%
! AAGAATGGAAAATGAAGAAA+AGG - chr1.1:75444320-75444339 None:intergenic 25.0%
! ACAAAACAAAGATACAGATT+TGG + chr1.1:75444961-75444980 MS.gene49973:CDS 25.0%
! CATTCTTATATTGCTCTTAT+TGG + chr1.1:75445391-75445410 MS.gene49973:intron 25.0%
! CTAGCAATGATAATTAAACT+AGG - chr1.1:75445844-75445863 None:intergenic 25.0%
! GGATTCATAAAAACACAAAA+AGG - chr1.1:75445119-75445138 None:intergenic 25.0%
! TGTTTGCAAATATGGTAATA+GGG + chr1.1:75445456-75445475 MS.gene49973:intron 25.0%
! TTTCAACCACAACAAAAAAT+TGG + chr1.1:75446260-75446279 MS.gene49973:CDS 25.0%
!! AAATACTCTAAGAGTTTGTT+CGG + chr1.1:75444624-75444643 MS.gene49973:intron 25.0%
!!! AAACTTACTTTTGGATAGTT+TGG + chr1.1:75444726-75444745 MS.gene49973:intron 25.0%
!!! ATAGAATTAATTTTGGGACT+AGG + chr1.1:75444790-75444809 MS.gene49973:intron 25.0%
!!! GAACTTGATTTTGAGTTTTT+GGG + chr1.1:75445217-75445236 MS.gene49973:CDS 25.0%
!!! TGAACTTGATTTTGAGTTTT+TGG + chr1.1:75445216-75445235 MS.gene49973:CDS 25.0%
!!! TGTGTTTTTATGAATCCAAT+TGG + chr1.1:75445122-75445141 MS.gene49973:intron 25.0%
!!! TTAAGACTTTTTGTTTTTGG+TGG + chr1.1:75445553-75445572 MS.gene49973:intron 25.0%
AAATCAAGTTCATCTCTTTC+TGG - chr1.1:75445208-75445227 None:intergenic 30.0%
AACAAAGATACAGATTTGGA+TGG + chr1.1:75444965-75444984 MS.gene49973:CDS 30.0%
AACCACAACAAAAAATTGGT+GGG + chr1.1:75446264-75446283 MS.gene49973:CDS 30.0%
AAGAATGAACTTACACTATC+TGG - chr1.1:75445378-75445397 None:intergenic 30.0%
AAGTCAAACATTATTCTGGA+AGG + chr1.1:75444862-75444881 MS.gene49973:intron 30.0%
ACACTTGAAAACAAGTACAT+TGG - chr1.1:75446109-75446128 None:intergenic 30.0%
ACTGAATGTTGCAAATGAAT+TGG + chr1.1:75446001-75446020 MS.gene49973:intron 30.0%
ATCAACACTAAAGTCTTTGT+AGG - chr1.1:75446206-75446225 None:intergenic 30.0%
ATCTGTACCGTTGAAATATA+AGG - chr1.1:75445933-75445952 None:intergenic 30.0%
ATGATAATAAGCAGCTACTA+TGG - chr1.1:75444356-75444375 None:intergenic 30.0%
CAAACAAATGTGTACAAGAA+TGG + chr1.1:75445268-75445287 MS.gene49973:CDS 30.0%
GAGTAAGTCAAACATTATTC+TGG + chr1.1:75444858-75444877 MS.gene49973:intron 30.0%
GTGTTTGCAAATATGGTAAT+AGG + chr1.1:75445455-75445474 MS.gene49973:intron 30.0%
GTTATTCCAGAGAATAGAAT+AGG - chr1.1:75445353-75445372 None:intergenic 30.0%
GTTGAAACACATTTAGGAAA+TGG - chr1.1:75446247-75446266 None:intergenic 30.0%
TAATGACTTAGATTTGACAC+TGG - chr1.1:75445636-75445655 None:intergenic 30.0%
TCATTTGGGAAGAAATTGTT+TGG - chr1.1:75446082-75446101 None:intergenic 30.0%
TTGACAATTGAGAAAAGAGA+AGG - chr1.1:75445524-75445543 None:intergenic 30.0%
TTGTTCTTAAACACCCTTAT+TGG - chr1.1:75446052-75446071 None:intergenic 30.0%
TTTGGTGGAAATAATAGTGT+AGG + chr1.1:75445568-75445587 MS.gene49973:intron 30.0%
! AGTGTTGTTAACAAGAATAG+GGG + chr1.1:75445148-75445167 MS.gene49973:intron 30.0%
! CATCAAAGTGTTTGCAAATA+TGG + chr1.1:75445448-75445467 MS.gene49973:intron 30.0%
! GAGTGTTGTTAACAAGAATA+GGG + chr1.1:75445147-75445166 MS.gene49973:intron 30.0%
! GTTTTTATGAATCCAATTGG+TGG + chr1.1:75445125-75445144 MS.gene49973:intron 30.0%
!! AAGTACATTGGTTTCTCATT+TGG - chr1.1:75446097-75446116 None:intergenic 30.0%
!! AGTACATTGGTTTCTCATTT+GGG - chr1.1:75446096-75446115 None:intergenic 30.0%
!! ATCAGAAAATGGATTATGCT+TGG + chr1.1:75446317-75446336 MS.gene49973:CDS 30.0%
!! GCATTTAATTTTCAATGGGT+AGG + chr1.1:75445708-75445727 MS.gene49973:intron 30.0%
!! TAAAGCATTGAATGTTTGAC+AGG + chr1.1:75444925-75444944 MS.gene49973:intron 30.0%
!! TCAGAAAATGGATTATGCTT+GGG + chr1.1:75446318-75446337 MS.gene49973:CDS 30.0%
!!! AATTGGAAACAGGTTTTTTG+TGG + chr1.1:75446018-75446037 MS.gene49973:intron 30.0%
!!! TGTACTTGTTTTCAAGTGTA+TGG + chr1.1:75446110-75446129 MS.gene49973:CDS 30.0%
!!! TTATGCTTTTGTCGGTTTAA+GGG + chr1.1:75445497-75445516 MS.gene49973:intron 30.0%
!!! TTTATGCTTTTGTCGGTTTA+AGG + chr1.1:75445496-75445515 MS.gene49973:intron 30.0%
AAGACAACTTCAGCATAATG+TGG + chr1.1:75444410-75444429 MS.gene49973:CDS 35.0%
AATGAACTTACACTATCTGG+TGG - chr1.1:75445375-75445394 None:intergenic 35.0%
AGTATGAAGCTGAAATTGGT+AGG + chr1.1:75444991-75445010 MS.gene49973:CDS 35.0%
ATATGGTAATAGGGCTAAGT+TGG + chr1.1:75445465-75445484 MS.gene49973:intron 35.0%
ATCACACCTATTCTATTCTC+TGG + chr1.1:75445344-75445363 MS.gene49973:CDS 35.0%
ATGTCATATGGACAGTAACA+AGG + chr1.1:75445884-75445903 MS.gene49973:intron 35.0%
ATTGAATGTTTGACAGGATG+TGG + chr1.1:75444931-75444950 MS.gene49973:intron 35.0%
CAACCACAACAAAAAATTGG+TGG + chr1.1:75446263-75446282 MS.gene49973:CDS 35.0%
CATCTGTAGAGGTAGTAAAA+TGG - chr1.1:75444444-75444463 None:intergenic 35.0%
GCATTAAAGTTGAGATTCTG+AGG + chr1.1:75444895-75444914 MS.gene49973:intron 35.0%
GTGGAAATAATAGTGTAGGA+CGG + chr1.1:75445572-75445591 MS.gene49973:intron 35.0%
GTGGACTTGAAAAAACAAAC+TGG + chr1.1:75446158-75446177 MS.gene49973:CDS 35.0%
GTTCAGTATGAAGCTGAAAT+TGG + chr1.1:75444987-75445006 MS.gene49973:CDS 35.0%
GTTGCAAATGAATTGGAAAC+AGG + chr1.1:75446008-75446027 MS.gene49973:intron 35.0%
GTTGTGGTTGAAACACATTT+AGG - chr1.1:75446253-75446272 None:intergenic 35.0%
TCTTTGTAGGATGACACAAA+TGG - chr1.1:75446193-75446212 None:intergenic 35.0%
TGCAATAGTCATAGATGACA+AGG - chr1.1:75446354-75446373 None:intergenic 35.0%
TGGAAATAATAGTGTAGGAC+GGG + chr1.1:75445573-75445592 MS.gene49973:intron 35.0%
TGTATGGAATGCAGATGATT+GGG + chr1.1:75446126-75446145 MS.gene49973:CDS 35.0%
TGTGGATAGAGTTCCAATAA+GGG + chr1.1:75446036-75446055 MS.gene49973:CDS 35.0%
TTGAATGTTTGACAGGATGT+GGG + chr1.1:75444932-75444951 MS.gene49973:intron 35.0%
TTGCAGATGTGTACAGAAAA+TGG + chr1.1:75445175-75445194 MS.gene49973:intron 35.0%
TTGTGGATAGAGTTCCAATA+AGG + chr1.1:75446035-75446054 MS.gene49973:CDS 35.0%
! CATTTTACTACCTCTACAGA+TGG + chr1.1:75444442-75444461 MS.gene49973:CDS 35.0%
! GCACATCTTTATGCTTTTGT+CGG + chr1.1:75445489-75445508 MS.gene49973:intron 35.0%
! TCTTTCAGGTGATCAGAAAA+TGG + chr1.1:75446306-75446325 MS.gene49973:CDS 35.0%
!! GGAGTGTTGTTAACAAGAAT+AGG + chr1.1:75445146-75445165 MS.gene49973:intron 35.0%
!! TAGTGTTGATGCTTGTCAAT+GGG + chr1.1:75446216-75446235 MS.gene49973:CDS 35.0%
!! TTAGTGTTGATGCTTGTCAA+TGG + chr1.1:75446215-75446234 MS.gene49973:CDS 35.0%
!!! ATCCCACCAATTTTTTGTTG+TGG - chr1.1:75446269-75446288 None:intergenic 35.0%
AAAACTGCAGACATTCTCAC+AGG - chr1.1:75445611-75445630 None:intergenic 40.0%
AATGTGTACAAGAATGGGAC+CGG + chr1.1:75445274-75445293 MS.gene49973:CDS 40.0%
ATGAAGCTGAAATTGGTAGG+AGG + chr1.1:75444994-75445013 MS.gene49973:CDS 40.0%
ATGTCTCATCTCACGATTAC+CGG - chr1.1:75445296-75445315 None:intergenic 40.0%
ATGTGTACAGAAAATGGTGC+AGG + chr1.1:75445181-75445200 MS.gene49973:CDS 40.0%
CTCTCATTAGACAATGACAC+AGG + chr1.1:75444478-75444497 MS.gene49973:CDS 40.0%
GTGAGATGAGACATATGCTT+TGG + chr1.1:75445302-75445321 MS.gene49973:CDS 40.0%
GTGTATGGAATGCAGATGAT+TGG + chr1.1:75446125-75446144 MS.gene49973:CDS 40.0%
GTTAACAACACTCCACCAAT+TGG - chr1.1:75445140-75445159 None:intergenic 40.0%
TATGATGCTTGGCATCTTTC+AGG + chr1.1:75446292-75446311 MS.gene49973:CDS 40.0%
TGTGTACAGAAAATGGTGCA+GGG + chr1.1:75445182-75445201 MS.gene49973:CDS 40.0%
TTCAATGGGTAGGATTTCAC+TGG + chr1.1:75445718-75445737 MS.gene49973:intron 40.0%
!! GAGTTTTTGGGAAACAGAAC+AGG + chr1.1:75445229-75445248 MS.gene49973:CDS 40.0%
!!! TTTTTCAAGTCCACCTCTTG+TGG - chr1.1:75446152-75446171 None:intergenic 40.0%
AATCTTGTCCTACACGGGAA+TGG - chr1.1:75445767-75445786 None:intergenic 45.0%
AGCAGAAGCAGCTATGTCTA+AGG + chr1.1:75444378-75444397 MS.gene49973:CDS 45.0%
ATAGGTGTGATAGTCCTCAG+TGG - chr1.1:75445335-75445354 None:intergenic 45.0%
CCTCACAATCTTGTCCTACA+CGG - chr1.1:75445773-75445792 None:intergenic 45.0%
CTCACAATCTTGTCCTACAC+GGG - chr1.1:75445772-75445791 None:intergenic 45.0%
GCAGAAGCAGCTATGTCTAA+GGG + chr1.1:75444379-75444398 MS.gene49973:CDS 45.0%
GGCTGCAAGGAAAAGAAGAA+TGG - chr1.1:75444335-75444354 None:intergenic 45.0%
GGTGGGATCAATATGATGCT+TGG + chr1.1:75446281-75446300 MS.gene49973:CDS 45.0%
TAGGTGTGATAGTCCTCAGT+GGG - chr1.1:75445334-75445353 None:intergenic 45.0%
! ACGTTTTCCAACTTTGCCTG+AGG + chr1.1:75446384-75446403 MS.gene49973:CDS 45.0%
AAGCAGCTACTATGGCTGCA+AGG - chr1.1:75444348-75444367 None:intergenic 50.0%
AATGGATCCTCTCCAGTGAC+TGG - chr1.1:75445749-75445768 None:intergenic 50.0%
CCAGATCTGTCCATCTGTAG+AGG - chr1.1:75444455-75444474 None:intergenic 50.0%
CCGTGTAGGACAAGATTGTG+AGG + chr1.1:75445770-75445789 MS.gene49973:intron 50.0%
CCTCTACAGATGGACAGATC+TGG + chr1.1:75444452-75444471 MS.gene49973:CDS 50.0%
TTGGTAGGAGGTGATTCTGC+TGG + chr1.1:75445006-75445025 MS.gene49973:CDS 50.0%
! GGAGTGCTCATTGAGTCCAT+GGG + chr1.1:75446405-75446424 MS.gene49973:CDS 50.0%
!! AGGAGTGCTCATTGAGTCCA+TGG + chr1.1:75446404-75446423 MS.gene49973:CDS 50.0%
!! GCTTTGGTTTGATCCCACTG+AGG + chr1.1:75445318-75445337 MS.gene49973:CDS 50.0%
GAGAGGATCCATTCCCGTGT+AGG + chr1.1:75445756-75445775 MS.gene49973:intron 55.0%
GAGCACTCCTCAGGCAAAGT+TGG - chr1.1:75446394-75446413 None:intergenic 55.0%
GATCCTCTCCAGTGACTGGA+GGG - chr1.1:75445745-75445764 None:intergenic 55.0%
GATGATTGGGCCACAAGAGG+TGG + chr1.1:75446139-75446158 MS.gene49973:CDS 55.0%
GCAGATGATTGGGCCACAAG+AGG + chr1.1:75446136-75446155 MS.gene49973:CDS 55.0%
GGACTCAATGAGCACTCCTC+AGG - chr1.1:75446403-75446422 None:intergenic 55.0%
GGATCCTCTCCAGTGACTGG+AGG - chr1.1:75445746-75445765 None:intergenic 60.0%
TCACTGGACCCTCCAGTCAC+TGG + chr1.1:75445734-75445753 MS.gene49973:intron 60.0%
GGACCCTCCAGTCACTGGAG+AGG + chr1.1:75445739-75445758 MS.gene49973:intron 65.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 75444304 75446432 75444304 ID=MS.gene49973
chr1.1 mRNA 75444304 75446432 75444304 ID=MS.gene49973.t1;Parent=MS.gene49973
chr1.1 exon 75444304 75444499 75444304 ID=MS.gene49973.t1.exon1;Parent=MS.gene49973.t1
chr1.1 CDS 75444304 75444499 75444304 ID=cds.MS.gene49973.t1;Parent=MS.gene49973.t1
chr1.1 exon 75444947 75445047 75444947 ID=MS.gene49973.t1.exon2;Parent=MS.gene49973.t1
chr1.1 CDS 75444947 75445047 75444947 ID=cds.MS.gene49973.t1;Parent=MS.gene49973.t1
chr1.1 exon 75445181 75445383 75445181 ID=MS.gene49973.t1.exon3;Parent=MS.gene49973.t1
chr1.1 CDS 75445181 75445383 75445181 ID=cds.MS.gene49973.t1;Parent=MS.gene49973.t1
chr1.1 exon 75446030 75446432 75446030 ID=MS.gene49973.t1.exon4;Parent=MS.gene49973.t1
chr1.1 CDS 75446030 75446432 75446030 ID=cds.MS.gene49973.t1;Parent=MS.gene49973.t1
Gene Sequence

>MS.gene49973

ATGGATGGAGGGTCCTTTTCTTCATTTTCCATTCTTCTTTTCCTTGCAGCCATAGTAGCTGCTTATTATCATTCAGCAGAAGCAGCTATGTCTAAGGGATCTTTTGAAGACAACTTCAGCATAATGTGGTCTGAAAACCATTTTACTACCTCTACAGATGGACAGATCTGGTATCTCTCATTAGACAATGACACAGGTATCAAATCAAATGCATGAGCATAACATTAAAATGAGCATTTTGTTGCACTATACACTCTATTATTATGTTATGTTTTCATTTGATTATTTTATGTAATCTTAAGTATATTTAAAATAGTACAAAATACTCTAAGAGTTTGTTCGGTTCTGCTGTGATAAAAATTGTTTTTGATTGAAATACAATGATTTATGTTTTTATACATTCATTAAAAAATGATTTACATAAACTTACTTTTGGATAGTTTGGATCAAAGTTACTTGTTTGTGTATGTATATATTAGCTTTTCGATAGAATTAATTTTGGGACTAGGATCAATTCTAAAGTTGAGAGTATAATTTATGGTCAAATCATGTTTGAGTAAGTCAAACATTATTCTGGAAGGTACAAATATAGCATTAAAGTTGAGATTCTGAGGCCATTTTTAAAGCATTGAATGTTTGACAGGATGTGGGTTTCAAACAAAACAAAGATACAGATTTGGATGGTTCAGTATGAAGCTGAAATTGGTAGGAGGTGATTCTGCTGGTGTTGTTACAGCATATTATGTAAGTAAACAATTACATTAATATTGAAATTTAGTACTAAATAGTAAATATAAATTTTTTGATGAGTTCCTTTTTGTGTTTTTATGAATCCAATTGGTGGAGTGTTGTTAACAAGAATAGGGGATTGTTGCAGATGTGTACAGAAAATGGTGCAGGGCCAGAAAGAGATGAACTTGATTTTGAGTTTTTGGGAAACAGAACAGGACAACCATATTTGATTCAAACAAATGTGTACAAGAATGGGACCGGTAATCGTGAGATGAGACATATGCTTTGGTTTGATCCCACTGAGGACTATCACACCTATTCTATTCTCTGGAATAACCACCAGATAGTGTAAGTTCATTCTTATATTGCTCTTATTGGTGTTCTTAATCTTATAACCCAAGTAAAAAAATGACATCAAAGTGTTTGCAAATATGGTAATAGGGCTAAGTTGGTGCACATCTTTATGCTTTTGTCGGTTTAAGGGTCCTTCTCTTTTCTCAATTGTCAAAAAAGTAGTTTAAGACTTTTTGTTTTTGGTGGAAATAATAGTGTAGGACGGGAATAGATTGTCTCCTGTGAGAATGTCTGCAGTTTTCTCCAGTGTCAAATCTAAGTCATTAAACATCTTCTATCTCTTGATTTTAACATTTATTAACATTCAATTTCTTAAATGCATTTAATTTTCAATGGGTAGGATTTCACTGGACCCTCCAGTCACTGGAGAGGATCCATTCCCGTGTAGGACAAGATTGTGAGGTTAAAGAAACGAATCTAGTTCAGTAAAATGTATGTACAATATGGTGGGCCTAGTTTAATTATCATTGCTAGCTGCTCATATGAATATGATTATGTCATATGGACAGTAACAAGGAAAGAGTGTTTCTTTTAAAAAGACCTTATATTTCAACGGTACAGATTATTTAAAATACCTTAATTCAAGATATTTATGGAATTTTTTTTTGTTAACTGAATGTTGCAAATGAATTGGAAACAGGTTTTTTGTGGATAGAGTTCCAATAAGGGTGTTTAAGAACAATGACAAACCAAACAATTTCTTCCCAAATGAGAAACCAATGTACTTGTTTTCAAGTGTATGGAATGCAGATGATTGGGCCACAAGAGGTGGACTTGAAAAAACAAACTGGAAATTAGCACCATTTGTGTCATCCTACAAAGACTTTAGTGTTGATGCTTGTCAATGGGAAGATCCATTTCCTAAATGTGTTTCAACCACAACAAAAAATTGGTGGGATCAATATGATGCTTGGCATCTTTCAGGTGATCAGAAAATGGATTATGCTTGGGTTCAAAGAAACCTTGTCATCTATGACTATTGCAATGATTCTGAACGTTTTCCAACTTTGCCTGAGGAGTGCTCATTGAGTCCATGGGAGTAA
Protein sequence

>MS.gene49973.t1

MDGGSFSSFSILLFLAAIVAAYYHSAEAAMSKGSFEDNFSIMWSENHFTTSTDGQIWYLSLDNDTGCGFQTKQRYRFGWFSMKLKLVGGDSAGVVTAYYMCTENGAGPERDELDFEFLGNRTGQPYLIQTNVYKNGTGNREMRHMLWFDPTEDYHTYSILWNNHQIVFFVDRVPIRVFKNNDKPNNFFPNEKPMYLFSSVWNADDWATRGGLEKTNWKLAPFVSSYKDFSVDACQWEDPFPKCVSTTTKNWWDQYDAWHLSGDQKMDYAWVQRNLVIYDYCNDSERFPTLPEECSLSPWE