AlfalfaGEDB | MS.gene50032

M. sativa cultivar XinJiangDaYe / MS.gene50032

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50032.t1	ABN08408.1	75.5	49	12	0	14	62	1	49	4.70E-09	70.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50032.t1	A2Q458	75.5	49	12	0	14	62	1	49	3.4e-09	70.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene50032	0.802931	4.44E-49	-1.69E-46
MS.gene050026	MS.gene50032	0.805295	1.43E-49	-1.69E-46
MS.gene050355	MS.gene50032	0.803607	3.22E-49	-1.69E-46
MS.gene051279	MS.gene50032	0.805423	1.34E-49	-1.69E-46
MS.gene051594	MS.gene50032	0.809371	1.96E-50	-1.69E-46
MS.gene051744	MS.gene50032	0.805587	1.24E-49	-1.69E-46
MS.gene052190	MS.gene50032	0.812525	4.06E-51	-1.69E-46
MS.gene052208	MS.gene50032	0.805704	1.17E-49	-1.69E-46
MS.gene052519	MS.gene50032	0.809535	1.80E-50	-1.69E-46
MS.gene052582	MS.gene50032	0.821022	5.03E-53	-1.69E-46
MS.gene052584	MS.gene50032	0.815976	7.02E-52	-1.69E-46
MS.gene053221	MS.gene50032	0.83452	2.86E-56	-1.69E-46
MS.gene053226	MS.gene50032	0.801755	7.75E-49	-1.69E-46
MS.gene05414	MS.gene50032	0.824063	9.89E-54	-1.69E-46
MS.gene05415	MS.gene50032	0.818344	2.06E-52	-1.69E-46
MS.gene054236	MS.gene50032	0.81649	5.38E-52	-1.69E-46
MS.gene054291	MS.gene50032	-0.820318	7.31E-53	-1.69E-46
MS.gene054468	MS.gene50032	0.812213	4.75E-51	-1.69E-46
MS.gene054651	MS.gene50032	0.803754	3.00E-49	-1.69E-46
MS.gene054775	MS.gene50032	0.804227	2.39E-49	-1.69E-46
MS.gene055778	MS.gene50032	0.829432	5.17E-55	-1.69E-46
MS.gene055818	MS.gene50032	0.823776	1.15E-53	-1.69E-46
MS.gene056173	MS.gene50032	0.821757	3.41E-53	-1.69E-46
MS.gene056366	MS.gene50032	0.80368	3.11E-49	-1.69E-46
MS.gene057012	MS.gene50032	0.814987	1.16E-51	-1.69E-46
MS.gene057026	MS.gene50032	0.802653	5.07E-49	-1.69E-46
MS.gene057027	MS.gene50032	0.893481	6.04E-75	-1.69E-46
MS.gene057414	MS.gene50032	0.810257	1.26E-50	-1.69E-46
MS.gene058037	MS.gene50032	0.84501	5.30E-59	-1.69E-46
MS.gene058625	MS.gene50032	0.819108	1.38E-52	-1.69E-46
MS.gene058636	MS.gene50032	0.806802	6.89E-50	-1.69E-46
MS.gene058829	MS.gene50032	0.828136	1.06E-54	-1.69E-46
MS.gene058979	MS.gene50032	0.805392	1.36E-49	-1.69E-46
MS.gene059272	MS.gene50032	0.806077	9.80E-50	-1.69E-46
MS.gene05954	MS.gene50032	0.825342	4.94E-54	-1.69E-46
MS.gene059584	MS.gene50032	0.802653	5.07E-49	-1.69E-46
MS.gene060521	MS.gene50032	0.810536	1.10E-50	-1.69E-46
MS.gene061040	MS.gene50032	-0.803845	2.87E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 11 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCAAGAACAGACCCGTTCT+TGG	0.335859	2.3:+3583505	MS.gene50032:CDS
ATTGAAACCGTAACAGATCT+TGG	0.395436	2.3:+3583426	MS.gene50032:CDS
TATTGGATCCGTGCCTTTGA+AGG	0.413281	2.3:-3583552	None:intergenic
CATGTCAGAAATCTTGCTTA+TGG	0.429523	2.3:-3583379	None:intergenic
TTCAAAGGCACGGATCCAAT+AGG	0.446448	2.3:+3583554	MS.gene50032:CDS
TGCTGATCCAAGATCTGTTA+CGG	0.482501	2.3:-3583433	None:intergenic
CTTGCTTATGGAAGCTTGCA+TGG	0.483544	2.3:-3583367	None:intergenic
TCAAAGGCACGGATCCAATA+GGG	0.491538	2.3:+3583555	MS.gene50032:CDS
GGATCCGTGCCTTTGAAGGT+AGG	0.550575	2.3:-3583548	None:intergenic
TACACCTACCTTCAAAGGCA+CGG	0.576905	2.3:+3583544	MS.gene50032:CDS
GAACGACACACTTACAAACT+CGG	0.620791	2.3:+3583464	MS.gene50032:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATTGAAACCGTAACAGATCT+TGG	+	chr2.3:3583426-3583445	MS.gene50032:CDS	35.0%
	CATGTCAGAAATCTTGCTTA+TGG	-	chr2.3:3583382-3583401	None:intergenic	35.0%
	GAACGACACACTTACAAACT+CGG	+	chr2.3:3583464-3583483	MS.gene50032:CDS	40.0%
	TGCTGATCCAAGATCTGTTA+CGG	-	chr2.3:3583436-3583455	None:intergenic	40.0%
!	GGTTTTACACCTACCTTCAA+AGG	+	chr2.3:3583539-3583558	MS.gene50032:CDS	40.0%
!!!	CAGCTTTTTCATCCAAGAAC+GGG	-	chr2.3:3583520-3583539	None:intergenic	40.0%
!!!	CCAGCTTTTTCATCCAAGAA+CGG	-	chr2.3:3583521-3583540	None:intergenic	40.0%
	ATCAAGAACAGACCCGTTCT+TGG	+	chr2.3:3583505-3583524	MS.gene50032:CDS	45.0%
	CCGTTCTTGGATGAAAAAGC+TGG	+	chr2.3:3583518-3583537	MS.gene50032:CDS	45.0%
	CTTGCTTATGGAAGCTTGCA+TGG	-	chr2.3:3583370-3583389	None:intergenic	45.0%
	TACACCTACCTTCAAAGGCA+CGG	+	chr2.3:3583544-3583563	MS.gene50032:CDS	45.0%
	TATTGGATCCGTGCCTTTGA+AGG	-	chr2.3:3583555-3583574	None:intergenic	45.0%
!	GGATCCGTGCCTTTGAAGGT+AGG	-	chr2.3:3583551-3583570	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	3583360	3583575	3583360	ID=MS.gene50032
chr2.3	mRNA	3583360	3583575	3583360	ID=MS.gene50032.t1;Parent=MS.gene50032
chr2.3	exon	3583360	3583575	3583360	ID=MS.gene50032.t1.exon1;Parent=MS.gene50032.t1
chr2.3	CDS	3583360	3583575	3583360	ID=cds.MS.gene50032.t1;Parent=MS.gene50032.t1

Gene Sequence	>MS.gene50032 ATGGATACCATGCAAGCTTCCATAAGCAAGATTTCTGACATGATCTCAAATCTTCAGCACAAAAACATTGAAACCGTAACAGATCTTGGATCAGCAGTCAAGAAGAACGACACACTTACAAACTCGGATCGAGATTCAGATGATGATCAAGAACAGACCCGTTCTTGGATGAAAAAGCTGGTTTTACACCTACCTTCAAAGGCACGGATCCAATAG
Protein sequence	>MS.gene50032.t1 MDTMQASISKISDMISNLQHKNIETVTDLGSAVKKNDTLTNSDRDSDDDQEQTRSWMKKLVLHLPSKARIQ