Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057027.t1 | ABN08408.1 | 75.5 | 49 | 12 | 0 | 14 | 62 | 1 | 49 | 4.70E-09 | 70.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057027.t1 | A2Q458 | 75.5 | 49 | 12 | 0 | 14 | 62 | 1 | 49 | 3.4e-09 | 70.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene057027 | 0.814005 | 1.92E-51 | -1.69E-46 |
| MS.gene049825 | MS.gene057027 | 0.810924 | 9.05E-51 | -1.69E-46 |
| MS.gene049873 | MS.gene057027 | 0.820058 | 8.38E-53 | -1.69E-46 |
| MS.gene050355 | MS.gene057027 | 0.81979 | 9.65E-53 | -1.69E-46 |
| MS.gene051957 | MS.gene057027 | 0.800084 | 1.70E-48 | -1.69E-46 |
| MS.gene052519 | MS.gene057027 | 0.803615 | 3.20E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene057027 | 0.816762 | 4.68E-52 | -1.69E-46 |
| MS.gene052584 | MS.gene057027 | 0.81491 | 1.21E-51 | -1.69E-46 |
| MS.gene052867 | MS.gene057027 | 0.828703 | 7.76E-55 | -1.69E-46 |
| MS.gene053226 | MS.gene057027 | 0.802577 | 5.25E-49 | -1.69E-46 |
| MS.gene05414 | MS.gene057027 | 0.819265 | 1.27E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene057027 | 0.830401 | 3.00E-55 | -1.69E-46 |
| MS.gene054651 | MS.gene057027 | 0.801742 | 7.80E-49 | -1.69E-46 |
| MS.gene054775 | MS.gene057027 | 0.813395 | 2.62E-51 | -1.69E-46 |
| MS.gene055778 | MS.gene057027 | 0.815274 | 1.01E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene057027 | 0.811278 | 7.58E-51 | -1.69E-46 |
| MS.gene057026 | MS.gene057027 | 0.811563 | 6.58E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene057414 | 0.827476 | 1.53E-54 | -1.69E-46 |
| MS.gene057027 | MS.gene057444 | 0.804876 | 1.75E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene058037 | 0.823674 | 1.22E-53 | -1.69E-46 |
| MS.gene057027 | MS.gene058625 | 0.823594 | 1.27E-53 | -1.69E-46 |
| MS.gene057027 | MS.gene058979 | 0.807285 | 5.45E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene059272 | 0.803311 | 3.70E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene05954 | 0.823196 | 1.58E-53 | -1.69E-46 |
| MS.gene057027 | MS.gene059625 | 0.800333 | 1.51E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene060521 | 0.823015 | 1.74E-53 | -1.69E-46 |
| MS.gene057027 | MS.gene060572 | 0.819445 | 1.16E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene062672 | 0.801213 | 1.00E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene06269 | 0.828988 | 6.62E-55 | -1.69E-46 |
| MS.gene057027 | MS.gene063417 | 0.818644 | 1.76E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene064321 | 0.815758 | 7.85E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene065347 | 0.804128 | 2.51E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene065793 | 0.815856 | 7.46E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene065904 | 0.801958 | 7.04E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene066335 | 0.807858 | 4.12E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene067208 | 0.807903 | 4.03E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene06723 | 0.805724 | 1.16E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene067932 | 0.805091 | 1.58E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene068385 | 0.819683 | 1.02E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene069956 | 0.811003 | 8.70E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene070627 | 0.805341 | 1.40E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene070818 | 0.812754 | 3.62E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene071322 | 0.82887 | 7.07E-55 | -1.69E-46 |
| MS.gene057027 | MS.gene071442 | 0.807405 | 5.14E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene071570 | 0.815815 | 7.62E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene072280 | 0.805282 | 1.44E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene072819 | 0.803863 | 2.85E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene073008 | 0.800936 | 1.14E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene073053 | 0.810522 | 1.11E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene073139 | 0.812337 | 4.46E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene07344 | 0.801205 | 1.01E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene07394 | 0.824353 | 8.45E-54 | -1.69E-46 |
| MS.gene057027 | MS.gene074320 | 0.803296 | 3.73E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene08553 | 0.804686 | 1.92E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene08554 | 0.815395 | 9.45E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene08853 | 0.818387 | 2.01E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene08854 | 0.807338 | 5.31E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene08862 | 0.804463 | 2.13E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene08882 | 0.800439 | 1.44E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene09233 | 0.826769 | 2.26E-54 | -1.69E-46 |
| MS.gene057027 | MS.gene09417 | 0.828905 | 6.93E-55 | -1.69E-46 |
| MS.gene057027 | MS.gene09952 | 0.802708 | 4.94E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene20809 | 0.807023 | 6.19E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene21927 | 0.803761 | 2.99E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene21929 | 0.808069 | 3.71E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene22255 | 0.805474 | 1.31E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene22270 | 0.800301 | 1.54E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene22271 | 0.801197 | 1.01E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene25151 | 0.802876 | 4.56E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene25755 | 0.807514 | 4.87E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene27059 | 0.814837 | 1.26E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene28204 | 0.801746 | 7.79E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene28514 | 0.801377 | 9.27E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene28652 | 0.815186 | 1.05E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene30085 | 0.825737 | 3.98E-54 | -1.69E-46 |
| MS.gene057027 | MS.gene31142 | 0.811675 | 6.22E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene31283 | 0.81742 | 3.33E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene31658 | 0.818459 | 1.94E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene32231 | 0.806615 | 7.55E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene32874 | 0.803959 | 2.72E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene33131 | 0.809106 | 2.23E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene33853 | 0.830456 | 2.91E-55 | -1.69E-46 |
| MS.gene057027 | MS.gene36496 | 0.803376 | 3.59E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene37612 | 0.804794 | 1.82E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene37944 | 0.830495 | 2.84E-55 | -1.69E-46 |
| MS.gene057027 | MS.gene39443 | 0.803077 | 4.14E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene39955 | 0.802941 | 4.42E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene40348 | 0.814786 | 1.29E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene40709 | 0.814212 | 1.73E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene40711 | 0.800141 | 1.66E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene44356 | 0.803326 | 3.68E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene44510 | 0.800358 | 1.50E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene44986 | 0.800421 | 1.45E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene45507 | 0.813537 | 2.43E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene47851 | 0.80524 | 1.47E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene48389 | 0.808473 | 3.04E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene48490 | 0.805779 | 1.13E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene50032 | 0.893481 | 6.04E-75 | -1.69E-46 |
| MS.gene057027 | MS.gene50546 | 0.821479 | 3.95E-53 | -1.69E-46 |
| MS.gene057027 | MS.gene51511 | 0.811016 | 8.64E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene51836 | 0.805497 | 1.30E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene52130 | 0.826755 | 2.28E-54 | -1.69E-46 |
| MS.gene057027 | MS.gene52396 | 0.801465 | 8.89E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene52671 | 0.830698 | 2.54E-55 | -1.69E-46 |
| MS.gene057027 | MS.gene53064 | 0.805312 | 1.42E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene53586 | 0.803746 | 3.01E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene53924 | 0.820723 | 5.90E-53 | -1.69E-46 |
| MS.gene057027 | MS.gene54544 | 0.807156 | 5.80E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene54656 | 0.800516 | 1.39E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene54686 | 0.812101 | 5.02E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene56468 | 0.816309 | 5.91E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene58547 | 0.831679 | 1.46E-55 | -1.69E-46 |
| MS.gene057027 | MS.gene58802 | 0.812984 | 3.22E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene61236 | 0.800411 | 1.46E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene63811 | 0.817447 | 3.28E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene64249 | 0.825291 | 5.08E-54 | -1.69E-46 |
| MS.gene057027 | MS.gene64670 | 0.80147 | 8.87E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene66474 | 0.815121 | 1.09E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene67746 | 0.809896 | 1.51E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene68824 | 0.807017 | 6.21E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene68826 | 0.808184 | 3.51E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene68904 | 0.807048 | 6.12E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene68999 | 0.81147 | 6.89E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene69116 | 0.803975 | 2.70E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene70515 | 0.811829 | 5.76E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene71704 | 0.800706 | 1.27E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene72268 | 0.805784 | 1.13E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene74257 | 0.821246 | 4.47E-53 | -1.69E-46 |
| MS.gene057027 | MS.gene74370 | 0.800259 | 1.57E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene74869 | 0.817639 | 2.97E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene75003 | 0.804956 | 1.68E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene75909 | 0.806463 | 8.13E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene75963 | 0.802127 | 6.50E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene76148 | 0.817993 | 2.47E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene76158 | 0.832966 | 6.99E-56 | -1.69E-46 |
| MS.gene057027 | MS.gene77588 | 0.823549 | 1.30E-53 | -1.69E-46 |
| MS.gene057027 | MS.gene77781 | 0.812097 | 5.03E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene80712 | 0.804012 | 2.65E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene80853 | 0.826801 | 2.22E-54 | -1.69E-46 |
| MS.gene057027 | MS.gene81047 | 0.808287 | 3.34E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene81334 | 0.828409 | 9.14E-55 | -1.69E-46 |
| MS.gene057027 | MS.gene81375 | 0.81132 | 7.43E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene83091 | 0.801417 | 9.09E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene84732 | 0.819176 | 1.33E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene84803 | 0.816607 | 5.07E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene84865 | 0.826243 | 3.02E-54 | -1.69E-46 |
| MS.gene057027 | MS.gene86086 | 0.821636 | 3.63E-53 | -1.69E-46 |
| MS.gene057027 | MS.gene87377 | 0.818367 | 2.03E-52 | -1.69E-46 |
| MS.gene057027 | MS.gene88091 | 0.807886 | 4.06E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene90022 | 0.811514 | 6.74E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene90146 | 0.800279 | 1.55E-48 | -1.69E-46 |
| MS.gene057027 | MS.gene90605 | 0.80394 | 2.74E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene91310 | 0.815204 | 1.04E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene91676 | 0.802092 | 6.61E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene92059 | 0.805129 | 1.55E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene93690 | 0.803766 | 2.98E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene93764 | 0.807738 | 4.37E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene97766 | 0.808037 | 3.77E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene98087 | 0.81316 | 2.95E-51 | -1.69E-46 |
| MS.gene057027 | MS.gene98088 | 0.807105 | 5.95E-50 | -1.69E-46 |
| MS.gene057027 | MS.gene98612 | 0.802288 | 6.02E-49 | -1.69E-46 |
| MS.gene057027 | MS.gene99849 | 0.812969 | 3.24E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAAGAACAGACCCGTTCT+TGG | 0.335859 | 2.1:+4569204 | MS.gene057027:CDS |
| ATTGAAACCGTAACAGATCT+TGG | 0.395436 | 2.1:+4569125 | MS.gene057027:CDS |
| TATTGGATCCGTGCCTTTGA+AGG | 0.413281 | 2.1:-4569251 | None:intergenic |
| CATGTCAGAAATCTTGCTTA+TGG | 0.429523 | 2.1:-4569078 | None:intergenic |
| TTCAAAGGCACGGATCCAAT+AGG | 0.446448 | 2.1:+4569253 | MS.gene057027:CDS |
| TGCTGATCCAAGATCTGTTA+CGG | 0.482501 | 2.1:-4569132 | None:intergenic |
| CTTGCTTATGGAAGCTTGCA+TGG | 0.483544 | 2.1:-4569066 | None:intergenic |
| TCAAAGGCACGGATCCAATA+GGG | 0.491538 | 2.1:+4569254 | MS.gene057027:CDS |
| GGATCCGTGCCTTTGAAGGT+AGG | 0.550575 | 2.1:-4569247 | None:intergenic |
| TACACCTACCTTCAAAGGCA+CGG | 0.576905 | 2.1:+4569243 | MS.gene057027:CDS |
| GAACGACACACTTACAAACT+CGG | 0.620791 | 2.1:+4569163 | MS.gene057027:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTGAAACCGTAACAGATCT+TGG | + | chr2.1:4569125-4569144 | MS.gene057027:CDS | 35.0% | |
| CATGTCAGAAATCTTGCTTA+TGG | - | chr2.1:4569081-4569100 | None:intergenic | 35.0% | |
| GAACGACACACTTACAAACT+CGG | + | chr2.1:4569163-4569182 | MS.gene057027:CDS | 40.0% | |
| TGCTGATCCAAGATCTGTTA+CGG | - | chr2.1:4569135-4569154 | None:intergenic | 40.0% | |
| ! | GGTTTTACACCTACCTTCAA+AGG | + | chr2.1:4569238-4569257 | MS.gene057027:CDS | 40.0% |
| !!! | CAGCTTTTTCATCCAAGAAC+GGG | - | chr2.1:4569219-4569238 | None:intergenic | 40.0% |
| !!! | CCAGCTTTTTCATCCAAGAA+CGG | - | chr2.1:4569220-4569239 | None:intergenic | 40.0% |
| ATCAAGAACAGACCCGTTCT+TGG | + | chr2.1:4569204-4569223 | MS.gene057027:CDS | 45.0% | |
| CCGTTCTTGGATGAAAAAGC+TGG | + | chr2.1:4569217-4569236 | MS.gene057027:CDS | 45.0% | |
| CTTGCTTATGGAAGCTTGCA+TGG | - | chr2.1:4569069-4569088 | None:intergenic | 45.0% | |
| TACACCTACCTTCAAAGGCA+CGG | + | chr2.1:4569243-4569262 | MS.gene057027:CDS | 45.0% | |
| TATTGGATCCGTGCCTTTGA+AGG | - | chr2.1:4569254-4569273 | None:intergenic | 45.0% | |
| ! | GGATCCGTGCCTTTGAAGGT+AGG | - | chr2.1:4569250-4569269 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 4569059 | 4569274 | 4569059 | ID=MS.gene057027 |
| chr2.1 | mRNA | 4569059 | 4569274 | 4569059 | ID=MS.gene057027.t1;Parent=MS.gene057027 |
| chr2.1 | exon | 4569059 | 4569274 | 4569059 | ID=MS.gene057027.t1.exon1;Parent=MS.gene057027.t1 |
| chr2.1 | CDS | 4569059 | 4569274 | 4569059 | ID=cds.MS.gene057027.t1;Parent=MS.gene057027.t1 |
| Gene Sequence |
| Protein sequence |
