AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50066

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50066.t1 MTR_4g069520 44.260 723 297 10 1 624 1 716 0.0 551
MS.gene50066.t1 MTR_4g069520 43.419 737 297 11 1 624 1 730 0.0 542
MS.gene50066.t1 MTR_4g069570 60.969 351 109 5 277 616 2 335 7.27e-147 428
MS.gene50066.t1 MTR_4g069550 50.734 477 171 11 180 624 26 470 1.00e-138 413
MS.gene50066.t1 MTR_4g069040 74.138 232 59 1 393 623 286 517 6.15e-127 384
MS.gene50066.t1 MTR_6g086530 69.712 208 62 1 413 619 1 208 1.91e-97 297
MS.gene50066.t1 MTR_3g116270 48.817 338 148 13 304 621 178 510 1.26e-95 303
MS.gene50066.t1 MTR_3g116320 54.789 261 114 3 363 621 434 692 2.68e-91 297
MS.gene50066.t1 MTR_3g107830 48.636 220 108 3 401 617 39 256 5.47e-69 225
MS.gene50066.t1 MTR_3g116410 47.511 221 111 3 401 618 59 277 1.36e-67 222
MS.gene50066.t1 MTR_3g116380 45.614 228 118 4 392 616 78 302 1.29e-63 212
MS.gene50066.t1 MTR_4g069680 46.364 220 113 3 401 617 70 287 2.68e-63 211
MS.gene50066.t1 MTR_4g068420 67.626 139 26 1 478 616 1 120 9.12e-61 198
MS.gene50066.t1 MTR_1g008500 36.073 219 130 7 401 615 171 383 4.06e-37 142
MS.gene50066.t1 MTR_3g109490 38.914 221 126 7 401 617 107 322 4.11e-36 138
MS.gene50066.t1 MTR_1g008470 35.185 216 131 5 401 614 89 297 1.97e-33 130
MS.gene50066.t1 MTR_0312s0030 33.486 218 135 6 401 615 113 323 2.59e-33 130
MS.gene50066.t1 MTR_1g007520 33.486 218 135 6 401 615 113 323 2.59e-33 130
MS.gene50066.t1 MTR_1g008510 33.796 216 136 5 401 615 115 324 1.34e-32 128
MS.gene50066.t1 MTR_1g008590 33.486 218 135 6 401 615 113 323 1.41e-32 128
MS.gene50066.t1 MTR_4g069540 65.909 88 27 1 377 464 13 97 3.08e-31 117
MS.gene50066.t1 MTR_1g008530 32.877 219 132 7 406 617 14 224 3.48e-31 121
MS.gene50066.t1 MTR_1g008460 34.112 214 133 5 401 614 89 294 3.85e-31 123
MS.gene50066.t1 MTR_1g008560 32.727 220 137 6 401 615 115 328 5.56e-31 124
MS.gene50066.t1 MTR_1g008450 34.061 229 138 7 392 614 89 310 4.44e-29 118
MS.gene50066.t1 MTR_5g034320 29.054 296 181 10 340 614 339 626 4.03e-27 117
MS.gene50066.t1 MTR_1g008580 32.386 176 112 5 441 615 1 170 1.32e-24 101
MS.gene50066.t1 MTR_8g064560 32.273 220 135 8 401 614 412 623 2.55e-24 108
MS.gene50066.t1 MTR_3g078090 28.111 217 148 5 401 614 413 624 3.93e-22 101
MS.gene50066.t1 MTR_8g064530 28.938 273 170 11 358 614 376 640 4.56e-22 101
MS.gene50066.t1 MTR_8g064500 30.366 191 127 5 425 614 185 370 1.58e-21 97.4
MS.gene50066.t1 MTR_4g133840 30.811 185 120 6 432 614 8 186 3.59e-19 86.3
MS.gene50066.t1 MTR_5g033350 28.643 199 132 6 424 619 12 203 3.90e-18 83.6
MS.gene50066.t1 MTR_5g033340 29.075 227 147 8 398 618 163 381 1.37e-16 82.4
MS.gene50066.t1 MTR_5g033340 31.788 151 95 4 446 594 6 150 6.15e-15 77.4
MS.gene50066.t1 MTR_5g033330 30.049 203 129 7 398 594 194 389 1.20e-15 80.9
MS.gene50066.t1 MTR_5g033330 31.788 151 95 4 446 594 37 181 1.87e-14 77.0
MS.gene50066.t1 MTR_5g033330 28.251 223 146 8 398 614 402 616 1.47e-13 74.3
MS.gene50066.t1 MTR_2g081770 31.050 219 121 10 401 616 79 270 1.57e-13 71.6
MS.gene50066.t1 MTR_8g445170 34.161 161 92 8 457 616 236 383 2.70e-12 69.3
MS.gene50066.t1 MTR_8g045880 34.437 151 85 8 467 616 177 314 1.74e-11 66.2
MS.gene50066.t1 MTR_8g045880 34.437 151 85 8 467 616 214 351 2.31e-11 66.2
MS.gene50066.t1 MTR_8g045880 34.437 151 85 8 467 616 212 349 2.33e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50066.t1 AT1G49320 34.926 272 153 6 360 615 15 278 3.49e-51 178
MS.gene50066.t1 AT5G25610 34.703 219 133 6 401 614 176 389 1.95e-35 138
MS.gene50066.t1 AT1G70370 29.545 220 141 7 401 614 411 622 1.51e-22 102
MS.gene50066.t1 AT1G70370 29.545 220 141 7 401 614 411 622 1.51e-22 102
MS.gene50066.t1 AT1G60390 28.182 220 144 7 401 614 409 620 1.94e-21 99.4
MS.gene50066.t1 AT1G23760 27.692 195 129 6 425 615 433 619 1.08e-18 90.5

Find 179 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCAAAATGGTGTAATTT+TGG 0.083271 4.2:-37208850 None:intergenic
TCTCCAAAATTACACCATTT+TGG 0.170910 4.2:+37208847 MS.gene50066:CDS
GGAAGTTGTGTAAGATCTTT+TGG 0.228867 4.2:-37207610 None:intergenic
CCATTGTAGGCAGCATTATA+AGG 0.237870 4.2:-37208104 None:intergenic
GAATTCGTTGACAAAGTAAT+TGG 0.254765 4.2:+37208767 MS.gene50066:CDS
ATTCAAGCAGCTTGGAATTA+AGG 0.259458 4.2:+37209072 MS.gene50066:CDS
TAAGGAGTTAGCACATATTT+TGG 0.279806 4.2:-37208732 None:intergenic
AAACCTCTTGAATGCGAAAT+TGG 0.291131 4.2:-37208550 None:intergenic
GGAAGCCATGTTTGATCCTT+TGG 0.307097 4.2:-37207826 None:intergenic
CAAAACTCATGCTCATGATT+TGG 0.334812 4.2:+37207536 MS.gene50066:CDS
TTGGCTCGAGGAGAATCTTC+AGG 0.344901 4.2:-37208656 None:intergenic
AGCTCTTGGCATTTGCCATT+TGG 0.347964 4.2:+37209018 MS.gene50066:CDS
ACCCACAAAAGATTTCTTAC+AGG 0.349441 4.2:-37209127 None:intergenic
GTCCATTGGATTCATGGGTA+TGG 0.353337 4.2:-37208352 None:intergenic
TGTTTGATCCTTTGGAATCA+TGG 0.360003 4.2:-37207818 None:intergenic
TATGACCCGTACTCATGATT+TGG 0.365784 4.2:+37207860 MS.gene50066:CDS
TGATGAGAATGGAGATAAAA+TGG 0.371870 4.2:+37208994 MS.gene50066:CDS
GAATTCAAGCATGGACTCTA+AGG 0.373206 4.2:-37208750 None:intergenic
AGCTGAGGGTGATGGGTTGT+TGG 0.373536 4.2:-37208819 None:intergenic
TAAGGCTCGTACTCATGATT+TGG 0.375651 4.2:+37207752 MS.gene50066:CDS
GGAAGTTGTGTAAGATCCTT+TGG 0.376639 4.2:-37207934 None:intergenic
TAGTAAATCGCATATGGATA+TGG 0.382186 4.2:-37208920 None:intergenic
TGTATGGTCCTTCGGAATCA+TGG 0.394695 4.2:-37207494 None:intergenic
TTGTAGAATGGCCAAGGAAT+TGG 0.397261 4.2:+37207354 MS.gene50066:intron
GTGTTCAAGGTTTCACTTGT+TGG 0.397973 4.2:+37208971 MS.gene50066:CDS
TGCTTGAATAGCTTTAGATC+TGG 0.398152 4.2:-37209058 None:intergenic
GGAAGTCGTGTAAGATCCTT+TGG 0.408259 4.2:-37208042 None:intergenic
ATGAGTACGAGCCTTATCTT+TGG 0.408288 4.2:-37207745 None:intergenic
GCCATTTGGACACATCTGAT+TGG 0.410666 4.2:+37209032 MS.gene50066:CDS
ATGAGCCTTATCTCCATTGT+AGG 0.411017 4.2:-37208171 None:intergenic
AAGTGTGTGTCTCATGGATT+TGG 0.414528 4.2:-37208675 None:intergenic
GTCATGTGATACTTCCAAAA+TGG 0.414847 4.2:-37208861 None:intergenic
TAAGGTGCCTCCTCATGATT+TGG 0.416358 4.2:+37207644 MS.gene50066:CDS
ATGATTCAAGTTGGAAGATC+GGG 0.423161 4.2:-37208462 None:intergenic
ACTTTAGATGATCTTAATGT+TGG 0.423544 4.2:+37208512 MS.gene50066:CDS
TGTGTGGTCCATTGGATTCA+TGG 0.429788 4.2:-37208358 None:intergenic
ACGGGCCTTATCTGTATTGT+AGG 0.438236 4.2:-37207955 None:intergenic
TGTAAGGTCCTTCAGAATCA+TGG 0.438353 4.2:-37208142 None:intergenic
AGAGGGATAGCTGAGGGTGA+TGG 0.442457 4.2:-37208827 None:intergenic
ATGACATTAATGCTCCCAAA+TGG 0.447741 4.2:+37208879 MS.gene50066:CDS
TACAGGGACTAAAGTGTTCA+AGG 0.447864 4.2:+37208958 MS.gene50066:CDS
ACAGGGAAGAAGTGGCAAAC+TGG 0.449165 4.2:-37209109 None:intergenic
GAGGGATAGCTGAGGGTGAT+GGG 0.455640 4.2:-37208826 None:intergenic
TAAGGCTCGCACTCATAACT+TGG 0.457652 4.2:+37208076 MS.gene50066:CDS
GATAACATTGTATGGTCCTT+CGG 0.457777 4.2:-37207502 None:intergenic
ATGAGTGATATACCCATTGT+AGG 0.457972 4.2:-37207901 None:intergenic
CCATTGTAGGCAGCATTGTA+AGG 0.460264 4.2:-37207672 None:intergenic
CCATTGTAGGCAGCATTGTA+AGG 0.460264 4.2:-37207996 None:intergenic
TGGAGTTGTGACATTGAGAA+AGG 0.461803 4.2:-37208608 None:intergenic
CCATTGTAGGCAACATTGTA+AGG 0.462042 4.2:-37208320 None:intergenic
GGTAGTGAGAATATTATGTT+TGG 0.463056 4.2:-37208798 None:intergenic
TGTATGGTCCGTCTAAATCA+TGG 0.465052 4.2:-37207440 None:intergenic
GGGATGGCAAGATACCCATT+TGG 0.466562 4.2:-37208894 None:intergenic
GTACAAGGCCCACACACAAC+TGG 0.470575 4.2:+37207393 MS.gene50066:CDS
CCATTGTATGCAGCATTGTA+AGG 0.470701 4.2:-37208212 None:intergenic
TATTGCCACTACATAGCTAC+AGG 0.470996 4.2:+37208941 MS.gene50066:CDS
TGGCAATAGTAAATCGCATA+TGG 0.471720 4.2:-37208926 None:intergenic
GGATGGCAAGATACCCATTT+GGG 0.471798 4.2:-37208893 None:intergenic
CCTTACAATGCTGCCTACAA+TGG 0.472378 4.2:+37207996 MS.gene50066:CDS
AGAAAAGTTGAAGAACACTT+TGG 0.472819 4.2:-37208628 None:intergenic
CCGTCGTATGCAGCATTGTA+TGG 0.474394 4.2:-37207780 None:intergenic
GCCCATTCAAATCATGAGTA+CGG 0.474912 4.2:-37207974 None:intergenic
TGTAAGATCCTTTGGAATCA+TGG 0.475100 4.2:-37208034 None:intergenic
AGCAACATTGTGTGGTCCAT+TGG 0.476581 4.2:-37208366 None:intergenic
AGTCTCTAGCACCAATTCCT+TGG 0.476779 4.2:-37207365 None:intergenic
CATACAGAGGCTCACAAGAA+TGG 0.477268 4.2:+37208482 MS.gene50066:CDS
CACACAATGTTGCTTACAAT+GGG 0.477978 4.2:+37208375 MS.gene50066:CDS
CATTGTAGGCAGCATTATAA+GGG 0.480428 4.2:-37208103 None:intergenic
TCCATTGGATTCATGGGTAT+GGG 0.481726 4.2:-37208351 None:intergenic
GCCCGTACTCATGATTTGAA+TGG 0.483308 4.2:+37207972 MS.gene50066:CDS
GTGCCTCCTCATGATTTGGA+TGG 0.483776 4.2:+37207648 MS.gene50066:CDS
CCCGTACTCATGATTTGAAT+GGG 0.486996 4.2:+37207973 MS.gene50066:CDS
TCTTACAATGCTGCCTACAA+TGG 0.487073 4.2:+37207564 MS.gene50066:CDS
TCTTACAATGCTGCCTACAA+TGG 0.487073 4.2:+37207888 MS.gene50066:CDS
ACTCATACTCACGATTCAAA+TGG 0.490442 4.2:+37208404 MS.gene50066:CDS
TCACTTGTTGGTGATGAGAA+TGG 0.493675 4.2:+37208983 MS.gene50066:CDS
ATGAGTGGTATAACCATTGT+AGG 0.495907 4.2:-37207685 None:intergenic
TGGCTCGAGGAGAATCTTCA+GGG 0.497335 4.2:-37208655 None:intergenic
CCTTATAATGCTGCCTACAA+TGG 0.497781 4.2:+37208104 MS.gene50066:CDS
GCTTGAATAGCTTTAGATCT+GGG 0.499001 4.2:-37209057 None:intergenic
CCATTGTAAGCAGCATTGTA+TGG 0.499097 4.2:-37207456 None:intergenic
AAGCAGCTTGGAATTAAGGC+TGG 0.500386 4.2:+37209076 MS.gene50066:CDS
ACCCGTCCAAATCATGAGTA+CGG 0.502387 4.2:-37207866 None:intergenic
TCCAATCAGATGTGTCCAAA+TGG 0.503560 4.2:-37209033 None:intergenic
ATGGGTGATATATCCATTGT+AGG 0.503598 4.2:-37208333 None:intergenic
CCATTGTAGGTAGTTGTGTA+AGG 0.504619 4.2:-37208158 None:intergenic
GTCCTTCAGAATCATGGGTA+CGG 0.506356 4.2:-37208136 None:intergenic
CCATACAATGCTGCTTACAA+TGG 0.507582 4.2:+37207456 MS.gene50066:CDS
ATGAGTGGTATACCCATTGT+AGG 0.507949 4.2:-37208009 None:intergenic
TGCCTCCTCATGATTTGGAT+GGG 0.509133 4.2:+37207649 MS.gene50066:CDS
GGAGATAAAATGGAAGCTCT+TGG 0.509573 4.2:+37209004 MS.gene50066:CDS
ATGAGCAGTATACCCATTGT+AGG 0.510388 4.2:-37207577 None:intergenic
CATACAATGCTGCTTACAAT+GGG 0.510573 4.2:+37207457 MS.gene50066:CDS
CCCACAAAAGATTTCTTACA+GGG 0.510673 4.2:-37209126 None:intergenic
CCTCACACAGGTGGCTACAA+TGG 0.512404 4.2:+37208266 MS.gene50066:CDS
TAGTTCAAGTGTGTGTCTCA+TGG 0.514802 4.2:-37208681 None:intergenic
ACGGGTGGTATATCCATTGT+AGG 0.515852 4.2:-37208117 None:intergenic
ACGAGCCTTATCTTTGGTGT+TGG 0.519256 4.2:-37207739 None:intergenic
GTGTGGTCCATTGGATTCAT+GGG 0.521013 4.2:-37208357 None:intergenic
CCACACAATGTTGCTTACAA+TGG 0.524425 4.2:+37208374 MS.gene50066:CDS
AACTACCTACAATGGAGATA+AGG 0.525612 4.2:+37208166 MS.gene50066:CDS
CTTACAATGCTGCCTACAAT+GGG 0.526874 4.2:+37207565 MS.gene50066:CDS
CTTACAATGCTGCCTACAAT+GGG 0.526874 4.2:+37207889 MS.gene50066:CDS
CTTACAATGCTGCCTACAAT+GGG 0.526874 4.2:+37207997 MS.gene50066:CDS
ACTCATAGCCATGATTCAAA+AGG 0.528864 4.2:+37207702 MS.gene50066:CDS
CTAAAGCTATTCAAGCAGCT+TGG 0.529380 4.2:+37209064 MS.gene50066:CDS
AACTTCCTACAGTAATGATA+AGG 0.530108 4.2:+37207626 MS.gene50066:CDS
TCCTTCAGAATCATGGGTAC+GGG 0.530443 4.2:-37208135 None:intergenic
TATGATTCAAGTTGGAAGAT+CGG 0.531065 4.2:-37208463 None:intergenic
CCCATTCAAATCATGAGTAC+GGG 0.534701 4.2:-37207973 None:intergenic
CTCATGCTCATGATTTGGAC+GGG 0.535553 4.2:+37207541 MS.gene50066:CDS
TGACATTAATGCTCCCAAAT+GGG 0.536817 4.2:+37208880 MS.gene50066:CDS
CCTTACAATGTTGCCTACAA+TGG 0.538533 4.2:+37208320 MS.gene50066:CDS
CCTTACAATGCTGCATACAA+TGG 0.538723 4.2:+37208212 MS.gene50066:CDS
CTCATACTTATGACGTGGAT+GGG 0.538997 4.2:+37208189 MS.gene50066:CDS
CTCGTACTCATGATTTGGAC+GGG 0.541778 4.2:+37207757 MS.gene50066:CDS
AACTTCCAACACCAAAGATA+AGG 0.547519 4.2:+37207734 MS.gene50066:CDS
GTATGGTCCTTCGGAATCAT+GGG 0.549177 4.2:-37207493 None:intergenic
CCTTACAATGCTGCCTACAA+TGG 0.549903 4.2:+37207672 MS.gene50066:CDS
GCTCATACTTATGACGTGGA+TGG 0.553154 4.2:+37208188 MS.gene50066:CDS
AGAAGCTGAAATGCTAGTGT+AGG 0.554357 4.2:-37206112 None:intergenic
GTGTTGTAGATAACATTGTA+TGG 0.556017 4.2:-37207510 None:intergenic
CCCGTACTCATGATTTGGAC+GGG 0.556910 4.2:+37207865 MS.gene50066:CDS
ATCGCATATGGATATGGAAG+GGG 0.557114 4.2:-37208914 None:intergenic
CCCGTCCAAATCATGAGTAC+GGG 0.561164 4.2:-37207865 None:intergenic
TTTGGATTCATGGGTATGTG+TGG 0.562685 4.2:-37208240 None:intergenic
ACCCGTACTCATGATTTGGA+CGG 0.566108 4.2:+37207864 MS.gene50066:CDS
GATTTCTTACAGGGAAGAAG+TGG 0.566923 4.2:-37209117 None:intergenic
GTAAGGTCCTTCAGAATCAT+GGG 0.570618 4.2:-37208141 None:intergenic
ACTCCAATTTCGCATTCAAG+AGG 0.571581 4.2:+37208547 MS.gene50066:CDS
TTGTGACATTGAGAAAGGAA+TGG 0.575099 4.2:-37208603 None:intergenic
GTATGGTCCGTCTAAATCAT+GGG 0.579468 4.2:-37207439 None:intergenic
GCATTATTGTAGAATGGCCA+AGG 0.579532 4.2:+37207348 MS.gene50066:intron
AAATCGCATATGGATATGGA+AGG 0.580949 4.2:-37208916 None:intergenic
TGTGATGATAAACCCATCAC+AGG 0.582158 4.2:+37208704 MS.gene50066:CDS
AACTTCCTACAATACAGATA+AGG 0.582500 4.2:+37207950 MS.gene50066:CDS
GCTAGAGACTTGAAAGTACA+AGG 0.582735 4.2:+37207378 MS.gene50066:CDS
CCATTGTAGCCACCTGTGTG+AGG 0.586581 4.2:-37208266 None:intergenic
CATACAATGCTGCATACGAC+GGG 0.586841 4.2:+37207781 MS.gene50066:CDS
TCATTGTAGCCAGTTGTGTG+TGG 0.586868 4.2:-37207402 None:intergenic
TTTGTCAACGAATTCAAGCA+TGG 0.589905 4.2:-37208759 None:intergenic
CCTTACACAACTACCTACAA+TGG 0.591066 4.2:+37208158 MS.gene50066:CDS
ACTCATGCTCATGATTTGGA+CGG 0.597559 4.2:+37207540 MS.gene50066:CDS
AGGCACCTTATCATTACTGT+AGG 0.600374 4.2:-37207631 None:intergenic
ACCCATACCCATGAATCCAA+TGG 0.602681 4.2:+37208350 MS.gene50066:CDS
CATTGTAGCCAGTTGTGTGT+GGG 0.603267 4.2:-37207401 None:intergenic
GCTCGTACTCATGATTTGGA+CGG 0.605381 4.2:+37207756 MS.gene50066:CDS
ACTCATAATCATGATTCCAA+AGG 0.608221 4.2:+37207918 MS.gene50066:CDS
ATTTGAATCGTGAGTATGAG+TGG 0.608503 4.2:-37208402 None:intergenic
AGCCTCTGTATGATTCAAGT+TGG 0.609127 4.2:-37208471 None:intergenic
ACGGGTCATATCTTTAGTGT+CGG 0.613379 4.2:-37207847 None:intergenic
ACTCAAACTCGTGATTTAGA+CGG 0.620513 4.2:+37208296 MS.gene50066:CDS
ACCCGTACCCATGATTCTGA+AGG 0.621860 4.2:+37208134 MS.gene50066:CDS
TTCGGAATCATGGGTACGAG+TGG 0.630046 4.2:-37207484 None:intergenic
TTCAGAATCATGGGTACGGG+TGG 0.630046 4.2:-37208132 None:intergenic
ACTCGTACCCATGATTTAGA+CGG 0.630479 4.2:+37207432 MS.gene50066:CDS
TGTCTCATGGATTTGGCTCG+AGG 0.632036 4.2:-37208668 None:intergenic
TAGTCCCTGTAGCTATGTAG+TGG 0.632642 4.2:-37208946 None:intergenic
TGATTCAAGTTGGAAGATCG+GGG 0.635303 4.2:-37208461 None:intergenic
CCATACAATGCTGCATACGA+CGG 0.635652 4.2:+37207780 MS.gene50066:CDS
AATCGCATATGGATATGGAA+GGG 0.645332 4.2:-37208915 None:intergenic
ACTCATAGCCATGATTCCAA+AGG 0.646157 4.2:+37207810 MS.gene50066:CDS
ACTCATAGCCATGATTCCAA+AGG 0.646157 4.2:+37208026 MS.gene50066:CDS
TTTGGAATCATGGCTATGAG+TGG 0.649158 4.2:-37207808 None:intergenic
TTTGGAATCATGGCTATGAG+TGG 0.649158 4.2:-37208024 None:intergenic
CCATTGTAAGCAACATTGTG+TGG 0.651516 4.2:-37208374 None:intergenic
GGCCCATCCAAATCATGAGG+AGG 0.652386 4.2:-37207651 None:intergenic
GTGATGATAAACCCATCACA+GGG 0.653846 4.2:+37208705 MS.gene50066:CDS
TGATTCCAAAGGATCAAACA+TGG 0.661194 4.2:+37207821 MS.gene50066:CDS
TAAGGCCCATCCAAATCATG+AGG 0.664395 4.2:-37207654 None:intergenic
ACTCGTACCCATGATTCCGA+AGG 0.667324 4.2:+37207486 MS.gene50066:CDS
GAATCATAGTACTCTTCACA+TGG 0.673977 4.2:-37209173 None:intergenic
CATATGGATATGGAAGGGGA+TGG 0.675097 4.2:-37208910 None:intergenic
TAAGGCTCATACTTATGACG+TGG 0.679385 4.2:+37208184 MS.gene50066:CDS
TTCCAACTTGAATCATACAG+AGG 0.685413 4.2:+37208469 MS.gene50066:CDS
GATTCAAGTTGGAAGATCGG+GGG 0.695599 4.2:-37208460 None:intergenic
ATTGCCACTACATAGCTACA+GGG 0.697067 4.2:+37208942 MS.gene50066:CDS
GCGATAGCATCAGAAGCCTG+AGG 0.710136 4.2:-37209151 None:intergenic
TGATGATAAACCCATCACAG+GGG 0.795789 4.2:+37208706 MS.gene50066:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAAACTTTCAATTCAA+CGG + chr4.2:37207185-37207204 MS.gene50066:intron 15.0%
!! AATTATAAGATATGTTATTG+AGG + chr4.2:37207070-37207089 MS.gene50066:intron 15.0%
!!! AAAATTATTTTATTGTGTCA+AGG - chr4.2:37207130-37207149 None:intergenic 15.0%
!!! TAAATATTTTACAAAACAGA+CGG - chr4.2:37206798-37206817 None:intergenic 15.0%
!!! TTTGTAAAATTTTACAGAAA+TGG + chr4.2:37207155-37207174 MS.gene50066:intron 15.0%
!! CAGAAAAATAAAAATAGTCA+CGG - chr4.2:37206318-37206337 None:intergenic 20.0%
!! TTATTAAGAAGTCTATTTGA+TGG + chr4.2:37206360-37206379 MS.gene50066:intron 20.0%
!! TTCTTAATAAAAACTAGTCA+CGG - chr4.2:37206350-37206369 None:intergenic 20.0%
!!! CTTTTTTAAAGTGAATAAGT+GGG + chr4.2:37206636-37206655 MS.gene50066:intron 20.0%
!!! TCACTTTGTATCTTTTTTTT+TGG + chr4.2:37206586-37206605 MS.gene50066:intron 20.0%
!!! TTTAGTTATTTTCTGCATTA+CGG + chr4.2:37206138-37206157 MS.gene50066:CDS 20.0%
!!! TTTTTTAAAGTGAATAAGTG+GGG + chr4.2:37206637-37206656 MS.gene50066:intron 20.0%
! AATGCCATAACAATAATTAG+TGG - chr4.2:37207333-37207352 None:intergenic 25.0%
! ACTTTAGATGATCTTAATGT+TGG + chr4.2:37208512-37208531 MS.gene50066:CDS 25.0%
! GAAGAAAATTCAGTTTGAAA+GGG + chr4.2:37206505-37206524 MS.gene50066:intron 25.0%
! GACGCTTAATAACATATTAT+AGG - chr4.2:37206564-37206583 None:intergenic 25.0%
! GACTTCCTACAATAAAAATA+AGG + chr4.2:37208058-37208077 MS.gene50066:CDS 25.0%
! TGAAGAAAATTCAGTTTGAA+AGG + chr4.2:37206504-37206523 MS.gene50066:intron 25.0%
!! CCACTTATTCACTTTAAAAA+AGG - chr4.2:37206638-37206657 None:intergenic 25.0%
!!! CCTTTTTTAAAGTGAATAAG+TGG + chr4.2:37206635-37206654 MS.gene50066:intron 25.0%
!!! TACATATTGATTTCGTTAGT+TGG + chr4.2:37207307-37207326 MS.gene50066:intron 25.0%
AAAAGTGGAAACATGACTAT+AGG - chr4.2:37206262-37206281 None:intergenic 30.0%
AACTTCCTACAATACAGATA+AGG + chr4.2:37207950-37207969 MS.gene50066:CDS 30.0%
AACTTCCTACAGTAATGATA+AGG + chr4.2:37207626-37207645 MS.gene50066:CDS 30.0%
ACGTTACCTTTCAAAAAAAG+AGG + chr4.2:37208574-37208593 MS.gene50066:CDS 30.0%
ACTCATAATCATGATTCCAA+AGG + chr4.2:37207918-37207937 MS.gene50066:CDS 30.0%
AGAAAAGTTGAAGAACACTT+TGG - chr4.2:37208631-37208650 None:intergenic 30.0%
CAGAAAAAGAAAAACTAGTC+AGG - chr4.2:37206287-37206306 None:intergenic 30.0%
GAATTCGTTGACAAAGTAAT+TGG + chr4.2:37208767-37208786 MS.gene50066:CDS 30.0%
GCATCATCAATGTAACAATT+GGG - chr4.2:37207266-37207285 None:intergenic 30.0%
GGTAGTGAGAATATTATGTT+TGG - chr4.2:37208801-37208820 None:intergenic 30.0%
GGTATATCATGCAACATTTA+GGG + chr4.2:37206190-37206209 MS.gene50066:intron 30.0%
GTGTTGTAGATAACATTGTA+TGG - chr4.2:37207513-37207532 None:intergenic 30.0%
GTTATGGCATTATTGTAGAA+TGG + chr4.2:37207342-37207361 MS.gene50066:intron 30.0%
TAGTAAATCGCATATGGATA+TGG - chr4.2:37208923-37208942 None:intergenic 30.0%
TGATGAGAATGGAGATAAAA+TGG + chr4.2:37208994-37209013 MS.gene50066:CDS 30.0%
TGCATCATCAATGTAACAAT+TGG - chr4.2:37207267-37207286 None:intergenic 30.0%
TGGTATATCATGCAACATTT+AGG + chr4.2:37206189-37206208 MS.gene50066:intron 30.0%
TTGGCCACTAATTATTGTTA+TGG + chr4.2:37207326-37207345 MS.gene50066:intron 30.0%
! AACACTTAACGACGTTTTAA+AGG + chr4.2:37206919-37206938 MS.gene50066:intron 30.0%
! ATCGTCAGTTTGTTCAAATT+TGG - chr4.2:37206991-37207010 None:intergenic 30.0%
! CTTCCAAAATGGTGTAATTT+TGG - chr4.2:37208853-37208872 None:intergenic 30.0%
! GACTTTTGAATCTTTTGCAT+TGG - chr4.2:37208441-37208460 None:intergenic 30.0%
! TAAGGAGTTAGCACATATTT+TGG - chr4.2:37208735-37208754 None:intergenic 30.0%
! TATTTTACAAAACAGACGGT+CGG - chr4.2:37206794-37206813 None:intergenic 30.0%
! TCTCCAAAATTACACCATTT+TGG + chr4.2:37208847-37208866 MS.gene50066:CDS 30.0%
! TGTAAGATCCTTTTGAATCA+TGG - chr4.2:37207713-37207732 None:intergenic 30.0%
!! AAAATGGTGTAATTTTGGAG+AGG - chr4.2:37208848-37208867 None:intergenic 30.0%
!! AAATGGTGTAATTTTGGAGA+GGG - chr4.2:37208847-37208866 None:intergenic 30.0%
!! CAGTTACATCAATGTTTTTG+AGG - chr4.2:37206829-37206848 None:intergenic 30.0%
!! TATGATTCAAGTTGGAAGAT+CGG - chr4.2:37208466-37208485 None:intergenic 30.0%
!!! CTGACTAGTTTTTCTTTTTC+TGG + chr4.2:37206285-37206304 MS.gene50066:intron 30.0%
!!! TGTAAGATCTTTTGGAATCA+TGG - chr4.2:37207605-37207624 None:intergenic 30.0%
AAAACTAGTCAGGCTAAAAG+TGG - chr4.2:37206277-37206296 None:intergenic 35.0%
AAACCTCTTGAATGCGAAAT+TGG - chr4.2:37208553-37208572 None:intergenic 35.0%
AAATCGCATATGGATATGGA+AGG - chr4.2:37208919-37208938 None:intergenic 35.0%
AACTACCTACAATGGAGATA+AGG + chr4.2:37208166-37208185 MS.gene50066:CDS 35.0%
AACTTCCAACACCAAAGATA+AGG + chr4.2:37207734-37207753 MS.gene50066:CDS 35.0%
AATCGCATATGGATATGGAA+GGG - chr4.2:37208918-37208937 None:intergenic 35.0%
ACACAGACATAAAAGAGAGA+AGG - chr4.2:37206857-37206876 None:intergenic 35.0%
ACATGCATTGTTATATGCAG+CGG - chr4.2:37206685-37206704 None:intergenic 35.0%
ACCCACAAAAGATTTCTTAC+AGG - chr4.2:37209130-37209149 None:intergenic 35.0%
ACTCAAACTCGTGATTTAGA+CGG + chr4.2:37208296-37208315 MS.gene50066:CDS 35.0%
ACTCATACTCACGATTCAAA+TGG + chr4.2:37208404-37208423 MS.gene50066:CDS 35.0%
ACTCATAGCCATGATTCAAA+AGG + chr4.2:37207702-37207721 MS.gene50066:CDS 35.0%
AGAACTGATCACCTTATGTA+TGG + chr4.2:37206528-37206547 MS.gene50066:intron 35.0%
ATGACATTAATGCTCCCAAA+TGG + chr4.2:37208879-37208898 MS.gene50066:CDS 35.0%
ATGAGTGATATACCCATTGT+AGG - chr4.2:37207904-37207923 None:intergenic 35.0%
ATGAGTGGTATAACCATTGT+AGG - chr4.2:37207688-37207707 None:intergenic 35.0%
ATGGGTGATATATCCATTGT+AGG - chr4.2:37208336-37208355 None:intergenic 35.0%
ATTCAAGCAGCTTGGAATTA+AGG + chr4.2:37209072-37209091 MS.gene50066:CDS 35.0%
ATTTGAATCGTGAGTATGAG+TGG - chr4.2:37208405-37208424 None:intergenic 35.0%
CAAAACTCATGCTCATGATT+TGG + chr4.2:37207536-37207555 MS.gene50066:CDS 35.0%
CATTGTAGGCAGCATTATAA+GGG - chr4.2:37208106-37208125 None:intergenic 35.0%
CCCACAAAAGATTTCTTACA+GGG - chr4.2:37209129-37209148 None:intergenic 35.0%
GATAACATTGTATGGTCCTT+CGG - chr4.2:37207505-37207524 None:intergenic 35.0%
GTACGAAGTTTCCATACATA+AGG - chr4.2:37206542-37206561 None:intergenic 35.0%
GTCATGTGATACTTCCAAAA+TGG - chr4.2:37208864-37208883 None:intergenic 35.0%
TGACATTAATGCTCCCAAAT+GGG + chr4.2:37208880-37208899 MS.gene50066:CDS 35.0%
TGATTCCAAAGGATCAAACA+TGG + chr4.2:37207821-37207840 MS.gene50066:CDS 35.0%
TGGCAATAGTAAATCGCATA+TGG - chr4.2:37208929-37208948 None:intergenic 35.0%
TTATACACGCTCGTATTATC+TGG + chr4.2:37206169-37206188 MS.gene50066:intron 35.0%
TTCCAACTTGAATCATACAG+AGG + chr4.2:37208469-37208488 MS.gene50066:CDS 35.0%
TTGTGACATTGAGAAAGGAA+TGG - chr4.2:37208606-37208625 None:intergenic 35.0%
TTTGTCAACGAATTCAAGCA+TGG - chr4.2:37208762-37208781 None:intergenic 35.0%
! CACACAATGTTGCTTACAAT+GGG + chr4.2:37208375-37208394 MS.gene50066:CDS 35.0%
! CATACAATGCTGCTTACAAT+GGG + chr4.2:37207457-37207476 MS.gene50066:CDS 35.0%
! CCCTGTAAGAAATCTTTTGT+GGG + chr4.2:37209126-37209145 MS.gene50066:CDS 35.0%
! GGAAGTTGTGTAAGATCTTT+TGG - chr4.2:37207613-37207632 None:intergenic 35.0%
! GTTTTGTACCAACAATGAGT+TGG + chr4.2:37206443-37206462 MS.gene50066:intron 35.0%
! TCCCTGTAAGAAATCTTTTG+TGG + chr4.2:37209125-37209144 MS.gene50066:CDS 35.0%
! TTTTGAATCATGGCTATGAG+TGG - chr4.2:37207703-37207722 None:intergenic 35.0%
!! ATGATTCAAGTTGGAAGATC+GGG - chr4.2:37208465-37208484 None:intergenic 35.0%
!! GCTTGAATAGCTTTAGATCT+GGG - chr4.2:37209060-37209079 None:intergenic 35.0%
!! TGCTTGAATAGCTTTAGATC+TGG - chr4.2:37209061-37209080 None:intergenic 35.0%
!! TGTAAGATCCTTTGGAATCA+TGG - chr4.2:37207929-37207948 None:intergenic 35.0%
!! TGTTTGATCCTTTGGAATCA+TGG - chr4.2:37207821-37207840 None:intergenic 35.0%
!! TTTTTGGTCAAGTAGTCTAG+TGG + chr4.2:37206602-37206621 MS.gene50066:intron 35.0%
!!! AGTCAGCCTCTTTTTTTGAA+AGG - chr4.2:37208583-37208602 None:intergenic 35.0%
!!! GCGAGCCTTATTTTTATTGT+AGG - chr4.2:37208066-37208085 None:intergenic 35.0%
!!! GTGAGGTTTTTTGGATTCAT+GGG - chr4.2:37208252-37208271 None:intergenic 35.0%
!!! TGTGAGGTTTTTTGGATTCA+TGG - chr4.2:37208253-37208272 None:intergenic 35.0%
AAGTGTGTGTCTCATGGATT+TGG - chr4.2:37208678-37208697 None:intergenic 40.0%
ACGGGTCATATCTTTAGTGT+CGG - chr4.2:37207850-37207869 None:intergenic 40.0%
ACTCATAGCCATGATTCCAA+AGG + chr4.2:37207810-37207829 MS.gene50066:CDS 40.0%
ACTCCAATTTCGCATTCAAG+AGG + chr4.2:37208547-37208566 MS.gene50066:CDS 40.0%
ACTCGTACCCATGATTTAGA+CGG + chr4.2:37207432-37207451 MS.gene50066:CDS 40.0%
AGAAGCTGAAATGCTAGTGT+AGG - chr4.2:37206115-37206134 None:intergenic 40.0%
AGCCTCTGTATGATTCAAGT+TGG - chr4.2:37208474-37208493 None:intergenic 40.0%
AGGCACCTTATCATTACTGT+AGG - chr4.2:37207634-37207653 None:intergenic 40.0%
ATCGCATATGGATATGGAAG+GGG - chr4.2:37208917-37208936 None:intergenic 40.0%
ATGAGCAGTATACCCATTGT+AGG - chr4.2:37207580-37207599 None:intergenic 40.0%
ATGAGTGGTATACCCATTGT+AGG - chr4.2:37208012-37208031 None:intergenic 40.0%
ATTGCCACTACATAGCTACA+GGG + chr4.2:37208942-37208961 MS.gene50066:CDS 40.0%
CACTTGTACCAACTCATTGT+TGG - chr4.2:37206454-37206473 None:intergenic 40.0%
CAGACATAAAAGAGAGAAGG+AGG - chr4.2:37206854-37206873 None:intergenic 40.0%
CCATTGTAAGCAACATTGTG+TGG - chr4.2:37208377-37208396 None:intergenic 40.0%
CCATTGTAAGCAGCATTGTA+TGG - chr4.2:37207459-37207478 None:intergenic 40.0%
CCATTGTAGGCAACATTGTA+AGG - chr4.2:37208323-37208342 None:intergenic 40.0%
CCATTGTAGGCAGCATTATA+AGG - chr4.2:37208107-37208126 None:intergenic 40.0%
CCATTGTAGGTAGTTGTGTA+AGG - chr4.2:37208161-37208180 None:intergenic 40.0%
CCATTGTATGCAGCATTGTA+AGG - chr4.2:37208215-37208234 None:intergenic 40.0%
CCCATTCAAATCATGAGTAC+GGG - chr4.2:37207976-37207995 None:intergenic 40.0%
CCCGTACTCATGATTTGAAT+GGG + chr4.2:37207973-37207992 MS.gene50066:CDS 40.0%
CCTTACAATGCTGCATACAA+TGG + chr4.2:37208212-37208231 MS.gene50066:CDS 40.0%
CCTTACAATGTTGCCTACAA+TGG + chr4.2:37208320-37208339 MS.gene50066:CDS 40.0%
CCTTACACAACTACCTACAA+TGG + chr4.2:37208158-37208177 MS.gene50066:CDS 40.0%
CCTTATAATGCTGCCTACAA+TGG + chr4.2:37208104-37208123 MS.gene50066:CDS 40.0%
CTAAAGCTATTCAAGCAGCT+TGG + chr4.2:37209064-37209083 MS.gene50066:CDS 40.0%
CTCATACTTATGACGTGGAT+GGG + chr4.2:37208189-37208208 MS.gene50066:CDS 40.0%
CTTACAATGCTGCCTACAAT+GGG + chr4.2:37207565-37207584 MS.gene50066:CDS 40.0%
GAATCCAAAAAACCTCACAC+AGG + chr4.2:37208254-37208273 MS.gene50066:CDS 40.0%
GAATTCAAGCATGGACTCTA+AGG - chr4.2:37208753-37208772 None:intergenic 40.0%
GAATTTGGACTCTCGAATTC+AGG + chr4.2:37206479-37206498 MS.gene50066:intron 40.0%
GATTTCTTACAGGGAAGAAG+TGG - chr4.2:37209120-37209139 None:intergenic 40.0%
GCATTATTGTAGAATGGCCA+AGG + chr4.2:37207348-37207367 MS.gene50066:intron 40.0%
GCCCATTCAAATCATGAGTA+CGG - chr4.2:37207977-37207996 None:intergenic 40.0%
GGAAGTTGTGTAAGATCCTT+TGG - chr4.2:37207721-37207740 None:intergenic 40.0%
GGAGATAAAATGGAAGCTCT+TGG + chr4.2:37209004-37209023 MS.gene50066:CDS 40.0%
GGTACAAGTGGCAAAGAATT+TGG + chr4.2:37206464-37206483 MS.gene50066:intron 40.0%
GTAAGGTCCTTCAGAATCAT+GGG - chr4.2:37208144-37208163 None:intergenic 40.0%
GTATGGTCCGTCTAAATCAT+GGG - chr4.2:37207442-37207461 None:intergenic 40.0%
GTCTTCTGCATTTATGCATC+TGG + chr4.2:37206225-37206244 MS.gene50066:intron 40.0%
GTGATGATAAACCCATCACA+GGG + chr4.2:37208705-37208724 MS.gene50066:CDS 40.0%
GTGTTCAAGGTTTCACTTGT+TGG + chr4.2:37208971-37208990 MS.gene50066:CDS 40.0%
TAAGGCTCATACTTATGACG+TGG + chr4.2:37208184-37208203 MS.gene50066:CDS 40.0%
TAAGGCTCGTACTCATGATT+TGG + chr4.2:37207752-37207771 MS.gene50066:CDS 40.0%
TAGTTCAAGTGTGTGTCTCA+TGG - chr4.2:37208684-37208703 None:intergenic 40.0%
TATGACCCGTACTCATGATT+TGG + chr4.2:37207860-37207879 MS.gene50066:CDS 40.0%
TATTGCCACTACATAGCTAC+AGG + chr4.2:37208941-37208960 MS.gene50066:CDS 40.0%
TCCAATCAGATGTGTCCAAA+TGG - chr4.2:37209036-37209055 None:intergenic 40.0%
TCCATTGGATTCATGGGTAT+GGG - chr4.2:37208354-37208373 None:intergenic 40.0%
TCTTACAATGCTGCCTACAA+TGG + chr4.2:37207564-37207583 MS.gene50066:CDS 40.0%
TGATGATAAACCCATCACAG+GGG + chr4.2:37208706-37208725 MS.gene50066:CDS 40.0%
TGGAGTTGTGACATTGAGAA+AGG - chr4.2:37208611-37208630 None:intergenic 40.0%
TGTAAGGTCCTTCAGAATCA+TGG - chr4.2:37208145-37208164 None:intergenic 40.0%
TGTATGGTCCGTCTAAATCA+TGG - chr4.2:37207443-37207462 None:intergenic 40.0%
TGTGATGATAAACCCATCAC+AGG + chr4.2:37208704-37208723 MS.gene50066:CDS 40.0%
TTGTAGAATGGCCAAGGAAT+TGG + chr4.2:37207354-37207373 MS.gene50066:intron 40.0%
TTTGGATTCATGGGTATGTG+TGG - chr4.2:37208243-37208262 None:intergenic 40.0%
! AAATCTTTTGTGGGTTCCTC+AGG + chr4.2:37209135-37209154 MS.gene50066:CDS 40.0%
! ACTCATGCTCATGATTTGGA+CGG + chr4.2:37207540-37207559 MS.gene50066:CDS 40.0%
! ATGAGCCTTATCTCCATTGT+AGG - chr4.2:37208174-37208193 None:intergenic 40.0%
! ATGAGTACGAGCCTTATCTT+TGG - chr4.2:37207748-37207767 None:intergenic 40.0%
! CCACACAATGTTGCTTACAA+TGG + chr4.2:37208374-37208393 MS.gene50066:CDS 40.0%
! CCATACAATGCTGCTTACAA+TGG + chr4.2:37207456-37207475 MS.gene50066:CDS 40.0%
! GCTAGAGACTTGAAAGTACA+AGG + chr4.2:37207378-37207397 MS.gene50066:CDS 40.0%
! TACAGGGACTAAAGTGTTCA+AGG + chr4.2:37208958-37208977 MS.gene50066:CDS 40.0%
! TTTGGAATCATGGCTATGAG+TGG - chr4.2:37207811-37207830 None:intergenic 40.0%
!! CAACAATGAGTTGGTACAAG+TGG + chr4.2:37206452-37206471 MS.gene50066:intron 40.0%
!! TCACTTGTTGGTGATGAGAA+TGG + chr4.2:37208983-37209002 MS.gene50066:CDS 40.0%
!! TGATTCAAGTTGGAAGATCG+GGG - chr4.2:37208464-37208483 None:intergenic 40.0%
AAGCAGCTTGGAATTAAGGC+TGG + chr4.2:37209076-37209095 MS.gene50066:CDS 45.0%
ACCCATACCCATGAATCCAA+TGG + chr4.2:37208350-37208369 MS.gene50066:CDS 45.0%
ACCCGTCCAAATCATGAGTA+CGG - chr4.2:37207869-37207888 None:intergenic 45.0%
ACGGGTGGTATATCCATTGT+AGG - chr4.2:37208120-37208139 None:intergenic 45.0%
AGCAACATTGTGTGGTCCAT+TGG - chr4.2:37208369-37208388 None:intergenic 45.0%
AGCTCTTGGCATTTGCCATT+TGG + chr4.2:37209018-37209037 MS.gene50066:CDS 45.0%
AGTCTCTAGCACCAATTCCT+TGG - chr4.2:37207368-37207387 None:intergenic 45.0%
CATACAATGCTGCATACGAC+GGG + chr4.2:37207781-37207800 MS.gene50066:CDS 45.0%
CATACAGAGGCTCACAAGAA+TGG + chr4.2:37208482-37208501 MS.gene50066:CDS 45.0%
CATATGGATATGGAAGGGGA+TGG - chr4.2:37208913-37208932 None:intergenic 45.0%
CATTGTAGCCAGTTGTGTGT+GGG - chr4.2:37207404-37207423 None:intergenic 45.0%
CATTGTTATATGCAGCGGTC+GGG - chr4.2:37206680-37206699 None:intergenic 45.0%
CCATACAATGCTGCATACGA+CGG + chr4.2:37207780-37207799 MS.gene50066:CDS 45.0%
CCATTGTAGGCAGCATTGTA+AGG - chr4.2:37207567-37207586 None:intergenic 45.0%
CCTTACAATGCTGCCTACAA+TGG + chr4.2:37207672-37207691 MS.gene50066:CDS 45.0%
GCATTGTTATATGCAGCGGT+CGG - chr4.2:37206681-37206700 None:intergenic 45.0%
GCCATTTGGACACATCTGAT+TGG + chr4.2:37209032-37209051 MS.gene50066:CDS 45.0%
GCCCGTACTCATGATTTGAA+TGG + chr4.2:37207972-37207991 MS.gene50066:CDS 45.0%
GCTCATACTTATGACGTGGA+TGG + chr4.2:37208188-37208207 MS.gene50066:CDS 45.0%
GGAAGCCATGTTTGATCCTT+TGG - chr4.2:37207829-37207848 None:intergenic 45.0%
GGAAGTCGTGTAAGATCCTT+TGG - chr4.2:37208045-37208064 None:intergenic 45.0%
GGATGGCAAGATACCCATTT+GGG - chr4.2:37208896-37208915 None:intergenic 45.0%
GTATGGTCCTTCGGAATCAT+GGG - chr4.2:37207496-37207515 None:intergenic 45.0%
GTCCATTGGATTCATGGGTA+TGG - chr4.2:37208355-37208374 None:intergenic 45.0%
GTCCTTCAGAATCATGGGTA+CGG - chr4.2:37208139-37208158 None:intergenic 45.0%
GTGTGGTCCATTGGATTCAT+GGG - chr4.2:37208360-37208379 None:intergenic 45.0%
TAAGGCCCATCCAAATCATG+AGG - chr4.2:37207657-37207676 None:intergenic 45.0%
TAAGGCTCGCACTCATAACT+TGG + chr4.2:37208076-37208095 MS.gene50066:CDS 45.0%
TAGTCCCTGTAGCTATGTAG+TGG - chr4.2:37208949-37208968 None:intergenic 45.0%
TCATTGTAGCCAGTTGTGTG+TGG - chr4.2:37207405-37207424 None:intergenic 45.0%
TCCAAAAAACCTCACACAGG+TGG + chr4.2:37208257-37208276 MS.gene50066:CDS 45.0%
TCCTTCAGAATCATGGGTAC+GGG - chr4.2:37208138-37208157 None:intergenic 45.0%
TGTATGGTCCTTCGGAATCA+TGG - chr4.2:37207497-37207516 None:intergenic 45.0%
TGTGTGGTCCATTGGATTCA+TGG - chr4.2:37208361-37208380 None:intergenic 45.0%
! ACCCGTACTCATGATTTGGA+CGG + chr4.2:37207864-37207883 MS.gene50066:CDS 45.0%
! ACGAGCCTTATCTTTGGTGT+TGG - chr4.2:37207742-37207761 None:intergenic 45.0%
! ACGGGCCTTATCTGTATTGT+AGG - chr4.2:37207958-37207977 None:intergenic 45.0%
! CTCATGCTCATGATTTGGAC+GGG + chr4.2:37207541-37207560 MS.gene50066:CDS 45.0%
! CTCGTACTCATGATTTGGAC+GGG + chr4.2:37207757-37207776 MS.gene50066:CDS 45.0%
! GCTCGTACTCATGATTTGGA+CGG + chr4.2:37207756-37207775 MS.gene50066:CDS 45.0%
! TAAGGTGCCTCCTCATGATT+TGG + chr4.2:37207644-37207663 MS.gene50066:CDS 45.0%
! TGCCTCCTCATGATTTGGAT+GGG + chr4.2:37207649-37207668 MS.gene50066:CDS 45.0%
!! ATTTTGGAGAGGGATAGCTG+AGG - chr4.2:37208837-37208856 None:intergenic 45.0%
!! ATTTTGGTCTCCCCTGTGAT+GGG - chr4.2:37208719-37208738 None:intergenic 45.0%
!! GATTCAAGTTGGAAGATCGG+GGG - chr4.2:37208463-37208482 None:intergenic 45.0%
!! TTTTGGAGAGGGATAGCTGA+GGG - chr4.2:37208836-37208855 None:intergenic 45.0%
!!! TATTTTGGTCTCCCCTGTGA+TGG - chr4.2:37208720-37208739 None:intergenic 45.0%
!!! TAAATATATTAGTTTTTTTT+AGG + chr4.2:37206385-37206404 MS.gene50066:intron 5.0%
ACAGGGAAGAAGTGGCAAAC+TGG - chr4.2:37209112-37209131 None:intergenic 50.0%
ACCCGTACCCATGATTCTGA+AGG + chr4.2:37208134-37208153 MS.gene50066:CDS 50.0%
ACTCGTACCCATGATTCCGA+AGG + chr4.2:37207486-37207505 MS.gene50066:CDS 50.0%
CCCGTCCAAATCATGAGTAC+GGG - chr4.2:37207760-37207779 None:intergenic 50.0%
CCGTCGTATGCAGCATTGTA+TGG - chr4.2:37207783-37207802 None:intergenic 50.0%
GGGATGGCAAGATACCCATT+TGG - chr4.2:37208897-37208916 None:intergenic 50.0%
TCAACCGAGCTATGCTCACA+GGG + chr4.2:37206710-37206729 MS.gene50066:intron 50.0%
TGAGCATAGCTCGGTTGACA+GGG - chr4.2:37206708-37206727 None:intergenic 50.0%
TGGCTCGAGGAGAATCTTCA+GGG - chr4.2:37208658-37208677 None:intergenic 50.0%
TTCAGAATCATGGGTACGGG+TGG - chr4.2:37208135-37208154 None:intergenic 50.0%
TTCGGAATCATGGGTACGAG+TGG - chr4.2:37207487-37207506 None:intergenic 50.0%
! AAGTGAATAAGTGGGGCGTC+CGG + chr4.2:37206644-37206663 MS.gene50066:intron 50.0%
! CCCGTACTCATGATTTGGAC+GGG + chr4.2:37207865-37207884 MS.gene50066:CDS 50.0%
! GTGCCTCCTCATGATTTGGA+TGG + chr4.2:37207648-37207667 MS.gene50066:CDS 50.0%
! TGTCTCATGGATTTGGCTCG+AGG - chr4.2:37208671-37208690 None:intergenic 50.0%
! TTGGCTCGAGGAGAATCTTC+AGG - chr4.2:37208659-37208678 None:intergenic 50.0%
!!! GCCACCTGTGTGAGGTTTTT+TGG - chr4.2:37208261-37208280 None:intergenic 50.0%
AGAGGGATAGCTGAGGGTGA+TGG - chr4.2:37208830-37208849 None:intergenic 55.0%
CCATTGTAGCCACCTGTGTG+AGG - chr4.2:37208269-37208288 None:intergenic 55.0%
CCTCACACAGGTGGCTACAA+TGG + chr4.2:37208266-37208285 MS.gene50066:CDS 55.0%
GAGGGATAGCTGAGGGTGAT+GGG - chr4.2:37208829-37208848 None:intergenic 55.0%
GCGATAGCATCAGAAGCCTG+AGG - chr4.2:37209154-37209173 None:intergenic 55.0%
GGCCCATCCAAATCATGAGG+AGG - chr4.2:37207654-37207673 None:intergenic 55.0%
GTACAAGGCCCACACACAAC+TGG + chr4.2:37207393-37207412 MS.gene50066:CDS 55.0%
GTCAACCGAGCTATGCTCAC+AGG + chr4.2:37206709-37206728 MS.gene50066:intron 55.0%
GTGAGCATAGCTCGGTTGAC+AGG - chr4.2:37206709-37206728 None:intergenic 55.0%
GTGTCCCTGTGAGCATAGCT+CGG - chr4.2:37206717-37206736 None:intergenic 55.0%
! AGTGAATAAGTGGGGCGTCC+GGG + chr4.2:37206645-37206664 MS.gene50066:intron 55.0%
!! AGCTGAGGGTGATGGGTTGT+TGG - chr4.2:37208822-37208841 None:intergenic 55.0%
! GTGAATAAGTGGGGCGTCCG+GGG + chr4.2:37206646-37206665 MS.gene50066:intron 60.0%
GCGGTCGGGAATTCGAACCC+CGG - chr4.2:37206666-37206685 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 37206106 37209180 37206106 ID=MS.gene50066
chr4.2 mRNA 37206106 37209180 37206106 ID=MS.gene50066.t1;Parent=MS.gene50066
chr4.2 exon 37206106 37206159 37206106 ID=MS.gene50066.t1.exon1;Parent=MS.gene50066.t1
chr4.2 CDS 37206106 37206159 37206106 ID=cds.MS.gene50066.t1;Parent=MS.gene50066.t1
chr4.2 exon 37207360 37209180 37207360 ID=MS.gene50066.t1.exon2;Parent=MS.gene50066.t1
chr4.2 CDS 37207360 37209180 37207360 ID=cds.MS.gene50066.t1;Parent=MS.gene50066.t1
Gene Sequence

>MS.gene50066

ATGGCTCCTACACTAGCATTTCAGCTTCTTGCTTTAGTTATTTTCTGCATTACGGTAATTTAATTATACACGCTCGTATTATCTGGTATATCATGCAACATTTAGGGCAATCTTCATATGTCTTCTGCATTTATGCATCTGGAATCAAAATTTCCTATAGTCATGTTTCCACTTTTAGCCTGACTAGTTTTTCTTTTTCTGGACTTTTACCGTGACTATTTTTATTTTTCTGAAAAATTTACCGTGACTAGTTTTTATTAAGAAGTCTATTTGATGGCTTAAATATATTAGTTTTTTTTAGGCAAACAAATATATTAATTTTATGTTTAGAAATGATGTTTTGTACCAACAATGAGTTGGTACAAGTGGCAAAGAATTTGGACTCTCGAATTCAGGTATGAAGAAAATTCAGTTTGAAAGGGAGAACTGATCACCTTATGTATGGAAACTTCGTACCTATAATATGTTATTAAGCGTCATTCACTTTGTATCTTTTTTTTTGGTCAAGTAGTCTAGTGGTTAGAATTCACCTTTTTTAAAGTGAATAAGTGGGGCGTCCGGGGTTCGAATTCCCGACCGCTGCATATAACAATGCATGTCCCTGTCAACCGAGCTATGCTCACAGGGACACTTTGTATCTTTAATTATAGAGATAAATTATATCATATAAACTTTTAATACACAACCGACCGTCTGTTTTGTAAAATATTTACTTACAGTCCTCAAAAACATTGATGTAACTGATCCTCCTTCTCTCTTTTATGTCTGTGTATACACACAGAGAGAGATATAAGAGTTAAGTTATATCGAAAGAACACTTAACGACGTTTTAAAGGTATCTTTTGTTGTGTAGTAGATTTTGTGTATCTTTGTAATTGTATACCAAATTTGAACAAACTGACGATTGAATATAAAATTTATATCATATATAGATCATTTATATGTTTTCTTTAAAAAAATCTAAAATTATAAGATATGTTATTGAGGTGAAACAATTTACATCTTAAGTATAAGACATTAACCTTGACACAATAAAATAATTTTAGATTTTTGTAAAATTTTACAGAAATGGATTGTATAATATAAACTTTCAATTCAACGGTCAATATGTATGTTTGTATGCAATATATAAAATGTTATTAAGCAATGTAACATAACCCAATTGTTACATTGATGATGCAATTTGATTTATCATTGTTTATACATATTGATTTCGTTAGTTGGCCACTAATTATTGTTATGGCATTATTGTAGAATGGCCAAGGAATTGGTGCTAGAGACTTGAAAGTACAAGGCCCACACACAACTGGCTACAATGAGTATAAGACTCGTACCCATGATTTAGACGGACCATACAATGCTGCTTACAATGGGTATACCACTCGTACCCATGATTCCGAAGGACCATACAATGTTATCTACAACACAGACAAAACTCATGCTCATGATTTGGACGGGTCTTACAATGCTGCCTACAATGGGTATACTGCTCATAACCATGATTCCAAAAGATCTTACACAACTTCCTACAGTAATGATAAGGTGCCTCCTCATGATTTGGATGGGCCTTACAATGCTGCCTACAATGGTTATACCACTCATAGCCATGATTCAAAAGGATCTTACACAACTTCCAACACCAAAGATAAGGCTCGTACTCATGATTTGGACGGGCCATACAATGCTGCATACGACGGGTATACCACTCATAGCCATGATTCCAAAGGATCAAACATGGCTTCCGACACTAAAGATATGACCCGTACTCATGATTTGGACGGGTCTTACAATGCTGCCTACAATGGGTATATCACTCATAATCATGATTCCAAAGGATCTTACACAACTTCCTACAATACAGATAAGGCCCGTACTCATGATTTGAATGGGCCTTACAATGCTGCCTACAATGGGTATACCACTCATAGCCATGATTCCAAAGGATCTTACACGACTTCCTACAATAAAAATAAGGCTCGCACTCATAACTTGGACGACCCTTATAATGCTGCCTACAATGGATATACCACCCGTACCCATGATTCTGAAGGACCTTACACAACTACCTACAATGGAGATAAGGCTCATACTTATGACGTGGATGGGCCTTACAATGCTGCATACAATGGATATACCACACATACCCATGAATCCAAAAAACCTCACACAGGTGGCTACAATGGAGATGAGACTCAAACTCGTGATTTAGACGGACCTTACAATGTTGCCTACAATGGATATATCACCCATACCCATGAATCCAATGGACCACACAATGTTGCTTACAATGGGTATACCACTCATACTCACGATTCAAATGGAGTCTCATCTACCAATGCAAAAGATTCAAAAGTCCCCCGATCTTCCAACTTGAATCATACAGAGGCTCACAAGAATGGATTTTTCACTTTAGATGATCTTAATGTTGGAAATGTGATGACACTCCAATTTCGCATTCAAGAGGTTTCACGTTACCTTTCAAAAAAAGAGGCTGACTCCATTCCTTTCTCAATGTCACAACTCCAAAGTGTTCTTCAACTTTTCTCAATCCCTGAAGATTCTCCTCGAGCCAAATCCATGAGACACACACTTGAACTATGTGATGATAAACCCATCACAGGGGAGACCAAAATATGTGCTAACTCCTTAGAGTCCATGCTTGAATTCGTTGACAAAGTAATTGGTTCAGACGCCAAACATAATATTCTCACTACCAACAACCCATCACCCTCAGCTATCCCTCTCCAAAATTACACCATTTTGGAAGTATCACATGACATTAATGCTCCCAAATGGGTATCTTGCCATCCCCTTCCATATCCATATGCGATTTACTATTGCCACTACATAGCTACAGGGACTAAAGTGTTCAAGGTTTCACTTGTTGGTGATGAGAATGGAGATAAAATGGAAGCTCTTGGCATTTGCCATTTGGACACATCTGATTGGAGCCCAGATCTAAAGCTATTCAAGCAGCTTGGAATTAAGGCTGGAAAGAATTCTCCAGTTTGCCACTTCTTCCCTGTAAGAAATCTTTTGTGGGTTCCTCAGGCTTCTGATGCTATCGCCATGTGA
Protein sequence

>MS.gene50066.t1

MAPTLAFQLLALVIFCITNGQGIGARDLKVQGPHTTGYNEYKTRTHDLDGPYNAAYNGYTTRTHDSEGPYNVIYNTDKTHAHDLDGSYNAAYNGYTAHNHDSKRSYTTSYSNDKVPPHDLDGPYNAAYNGYTTHSHDSKGSYTTSNTKDKARTHDLDGPYNAAYDGYTTHSHDSKGSNMASDTKDMTRTHDLDGSYNAAYNGYITHNHDSKGSYTTSYNTDKARTHDLNGPYNAAYNGYTTHSHDSKGSYTTSYNKNKARTHNLDDPYNAAYNGYTTRTHDSEGPYTTTYNGDKAHTYDVDGPYNAAYNGYTTHTHESKKPHTGGYNGDETQTRDLDGPYNVAYNGYITHTHESNGPHNVAYNGYTTHTHDSNGVSSTNAKDSKVPRSSNLNHTEAHKNGFFTLDDLNVGNVMTLQFRIQEVSRYLSKKEADSIPFSMSQLQSVLQLFSIPEDSPRAKSMRHTLELCDDKPITGETKICANSLESMLEFVDKVIGSDAKHNILTTNNPSPSAIPLQNYTILEVSHDINAPKWVSCHPLPYPYAIYYCHYIATGTKVFKVSLVGDENGDKMEALGICHLDTSDWSPDLKLFKQLGIKAGKNSPVCHFFPVRNLLWVPQASDAIAM