Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50578.t1 | AFK35192.1 | 97.1 | 137 | 4 | 0 | 1 | 137 | 1 | 137 | 1.40E-62 | 248.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50578.t1 | Q41387 | 59.9 | 137 | 55 | 0 | 1 | 137 | 1 | 137 | 1.2e-33 | 144.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50578.t1 | I3S4K0 | 97.1 | 137 | 4 | 0 | 1 | 137 | 1 | 137 | 1.0e-62 | 248.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049320 | MS.gene50578 | 0.813005 | 3.19E-51 | -1.69E-46 |
| MS.gene050163 | MS.gene50578 | 0.807456 | 5.01E-50 | -1.69E-46 |
| MS.gene050165 | MS.gene50578 | 0.800167 | 1.64E-48 | -1.69E-46 |
| MS.gene050612 | MS.gene50578 | 0.801036 | 1.09E-48 | -1.69E-46 |
| MS.gene050767 | MS.gene50578 | 0.825554 | 4.40E-54 | -1.69E-46 |
| MS.gene050770 | MS.gene50578 | 0.825065 | 5.74E-54 | -1.69E-46 |
| MS.gene050772 | MS.gene50578 | 0.803616 | 3.20E-49 | -1.69E-46 |
| MS.gene05100 | MS.gene50578 | 0.8035 | 3.39E-49 | -1.69E-46 |
| MS.gene051228 | MS.gene50578 | 0.812624 | 3.86E-51 | -1.69E-46 |
| MS.gene051692 | MS.gene50578 | 0.828926 | 6.85E-55 | -1.69E-46 |
| MS.gene051810 | MS.gene50578 | 0.849112 | 3.98E-60 | -1.69E-46 |
| MS.gene052271 | MS.gene50578 | 0.819971 | 8.77E-53 | -1.69E-46 |
| MS.gene053009 | MS.gene50578 | 0.809233 | 2.09E-50 | -1.69E-46 |
| MS.gene053207 | MS.gene50578 | 0.804762 | 1.85E-49 | -1.69E-46 |
| MS.gene053209 | MS.gene50578 | 0.800313 | 1.53E-48 | -1.69E-46 |
| MS.gene053632 | MS.gene50578 | 0.838652 | 2.53E-57 | -1.69E-46 |
| MS.gene053915 | MS.gene50578 | 0.813156 | 2.95E-51 | -1.69E-46 |
| MS.gene053916 | MS.gene50578 | 0.838334 | 3.06E-57 | -1.69E-46 |
| MS.gene054376 | MS.gene50578 | 0.813695 | 2.25E-51 | -1.69E-46 |
| MS.gene054600 | MS.gene50578 | 0.81432 | 1.64E-51 | -1.69E-46 |
| MS.gene054642 | MS.gene50578 | 0.805807 | 1.12E-49 | -1.69E-46 |
| MS.gene054802 | MS.gene50578 | 0.808557 | 2.92E-50 | -1.69E-46 |
| MS.gene054803 | MS.gene50578 | 0.804051 | 2.60E-49 | -1.69E-46 |
| MS.gene054804 | MS.gene50578 | 0.813853 | 2.07E-51 | -1.69E-46 |
| MS.gene055254 | MS.gene50578 | 0.859206 | 4.86E-63 | -1.69E-46 |
| MS.gene055362 | MS.gene50578 | 0.824266 | 8.86E-54 | -1.69E-46 |
| MS.gene055397 | MS.gene50578 | 0.828718 | 7.69E-55 | -1.69E-46 |
| MS.gene055499 | MS.gene50578 | 0.826598 | 2.48E-54 | -1.69E-46 |
| MS.gene05592 | MS.gene50578 | 0.806042 | 9.97E-50 | -1.69E-46 |
| MS.gene056374 | MS.gene50578 | 0.803216 | 3.87E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene50578 | 0.807372 | 5.22E-50 | -1.69E-46 |
| MS.gene057129 | MS.gene50578 | 0.853229 | 2.74E-61 | -1.69E-46 |
| MS.gene057295 | MS.gene50578 | 0.837049 | 6.54E-57 | -1.69E-46 |
| MS.gene057926 | MS.gene50578 | 0.801528 | 8.63E-49 | -1.69E-46 |
| MS.gene058006 | MS.gene50578 | 0.805361 | 1.39E-49 | -1.69E-46 |
| MS.gene058419 | MS.gene50578 | 0.806428 | 8.27E-50 | -1.69E-46 |
| MS.gene058723 | MS.gene50578 | 0.848501 | 5.89E-60 | -1.69E-46 |
| MS.gene059022 | MS.gene50578 | 0.805647 | 1.21E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene50578 | 0.811576 | 6.53E-51 | -1.69E-46 |
| MS.gene059718 | MS.gene50578 | 0.800464 | 1.42E-48 | -1.69E-46 |
| MS.gene061041 | MS.gene50578 | 0.801166 | 1.02E-48 | -1.69E-46 |
| MS.gene061200 | MS.gene50578 | 0.813473 | 2.51E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50578.t1 | MTR_1g070495 | 96.350 | 137 | 5 | 0 | 1 | 137 | 1 | 137 | 6.99e-93 | 265 |
| MS.gene50578.t1 | MTR_7g096150 | 76.642 | 137 | 31 | 1 | 1 | 137 | 1 | 136 | 8.83e-67 | 199 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50578.t1 | AT2G30570 | 63.043 | 138 | 45 | 3 | 1 | 137 | 1 | 133 | 7.29e-47 | 148 |
Find 41 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGTGTGTTCGGTCTTATC+TGG | 0.197833 | 1.2:+54115517 | MS.gene50578:CDS |
| GTTTCATCCTCTCCTGTTCT+TGG | 0.207350 | 1.2:+54114622 | MS.gene50578:CDS |
| TCCACCAATGCCATGGCTGC+TGG | 0.247914 | 1.2:-54115423 | None:intergenic |
| GGACTCAGCAACAACCTTCT+TGG | 0.302937 | 1.2:+54115483 | MS.gene50578:CDS |
| CTTCCCACCTTAGCCATTGT+TGG | 0.376821 | 1.2:-54115291 | None:intergenic |
| TTCATTTCAAGCAATTTAAA+GGG | 0.388432 | 1.2:-54115595 | None:intergenic |
| TAAGACCGAACACACCAAAA+AGG | 0.399989 | 1.2:-54115512 | None:intergenic |
| CCACCAATGCCATGGCTGCT+GGG | 0.401839 | 1.2:-54115422 | None:intergenic |
| CCCAGCAGCCATGGCATTGG+TGG | 0.403670 | 1.2:+54115422 | MS.gene50578:CDS |
| GAAGGAACCGGGCTTCCCTT+TGG | 0.425729 | 1.2:+54115462 | MS.gene50578:CDS |
| TTGCTTTCACTCACAGAAGA+AGG | 0.432700 | 1.2:-54115339 | None:intergenic |
| CTTGAAGAGGATGAAGAGTC+TGG | 0.434178 | 1.2:+54115567 | MS.gene50578:CDS |
| AGAATGAGCACTGAAGGAAC+CGG | 0.435090 | 1.2:+54115450 | MS.gene50578:CDS |
| TGGTGTGTTCGGTCTTATCT+GGG | 0.435857 | 1.2:+54115518 | MS.gene50578:CDS |
| AAGAACAACATACCAAGAAC+AGG | 0.443082 | 1.2:-54114634 | None:intergenic |
| AGCAACTCAAACATTGGAAT+GGG | 0.465387 | 1.2:+54115357 | MS.gene50578:CDS |
| TCAGCAACAACCTTCTTGGT+TGG | 0.473672 | 1.2:+54115487 | MS.gene50578:CDS |
| AGAGTGAGGTGTTCGATGGA+GGG | 0.491443 | 1.2:+54115312 | MS.gene50578:CDS |
| AGTGAAAGCAACTCAAACAT+TGG | 0.493591 | 1.2:+54115351 | MS.gene50578:CDS |
| AAGAGTGAGGTGTTCGATGG+AGG | 0.495227 | 1.2:+54115311 | MS.gene50578:CDS |
| GGGAAGAGTGAGGTGTTCGA+TGG | 0.511592 | 1.2:+54115308 | MS.gene50578:CDS |
| AAGCCCAGCAGCCATGGCAT+TGG | 0.535185 | 1.2:+54115419 | MS.gene50578:CDS |
| AAGCAACTCAAACATTGGAA+TGG | 0.541259 | 1.2:+54115356 | MS.gene50578:CDS |
| ATTGCCAACAATGGCTAAGG+TGG | 0.548615 | 1.2:+54115287 | MS.gene50578:CDS |
| AAGGTTGTTGCTGAGTCCAA+AGG | 0.556907 | 1.2:-54115478 | None:intergenic |
| TCTTTCATCCACCAATGCCA+TGG | 0.558482 | 1.2:-54115430 | None:intergenic |
| GCTGAGTCCAAAGGGAAGCC+CGG | 0.571088 | 1.2:-54115469 | None:intergenic |
| AGGATTGCCAACAATGGCTA+AGG | 0.571754 | 1.2:+54115284 | MS.gene50578:intron |
| AGGTTGTTGCTGAGTCCAAA+GGG | 0.582023 | 1.2:-54115477 | None:intergenic |
| GATGAAAGAATGAGCACTGA+AGG | 0.583467 | 1.2:+54115444 | MS.gene50578:CDS |
| GAATGAGCACTGAAGGAACC+GGG | 0.588005 | 1.2:+54115451 | MS.gene50578:CDS |
| CTACGCCTCGAGTCTTGAAG+AGG | 0.588236 | 1.2:+54115554 | MS.gene50578:CDS |
| TATGTCAAGCCCAGCAGCCA+TGG | 0.588442 | 1.2:+54115413 | MS.gene50578:CDS |
| AGTTGTAGTGGTAGCTGAGA+TGG | 0.594313 | 1.2:-54114554 | None:intergenic |
| TTATTCAGGATTGCCAACAA+TGG | 0.611540 | 1.2:+54115278 | MS.gene50578:intron |
| TTCATCCTCTTCAAGACTCG+AGG | 0.612569 | 1.2:-54115559 | None:intergenic |
| GGTGATCGATGCAGTTGTAG+TGG | 0.624319 | 1.2:-54114566 | None:intergenic |
| TTGCCAACAATGGCTAAGGT+GGG | 0.634166 | 1.2:+54115288 | MS.gene50578:CDS |
| CAACATACCAAGAACAGGAG+AGG | 0.652090 | 1.2:-54114629 | None:intergenic |
| TGGCTAAGGTGGGAAGAGTG+AGG | 0.666585 | 1.2:+54115298 | MS.gene50578:CDS |
| GGAGAGGATGAAACTGCAAG+AGG | 0.693611 | 1.2:-54114613 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGATATATATGTTTTAAGAT+TGG | + | chr1.2:54114771-54114790 | MS.gene50578:intron | 15.0% |
| !!! | TTATTTCATAACTTTTATTG+AGG | + | chr1.2:54115016-54115035 | MS.gene50578:intron | 15.0% |
| !!! | TTTTTCATCATTACAAAATT+GGG | - | chr1.2:54114962-54114981 | None:intergenic | 15.0% |
| !! | TATCATGCTATTCAATATTT+AGG | - | chr1.2:54114673-54114692 | None:intergenic | 20.0% |
| !!! | AACATTCAATCATTTTATTC+AGG | + | chr1.2:54115264-54115283 | MS.gene50578:intron | 20.0% |
| !!! | AATATTTAGGATGAAAATCA+AGG | - | chr1.2:54114660-54114679 | None:intergenic | 20.0% |
| !!! | ATTTGAACCATTTTATACTA+TGG | + | chr1.2:54115058-54115077 | MS.gene50578:intron | 20.0% |
| !!! | GTTTTTCATCATTACAAAAT+TGG | - | chr1.2:54114963-54114982 | None:intergenic | 20.0% |
| !!! | TATTTGTCGATTTTTTTTCT+CGG | + | chr1.2:54114983-54115002 | MS.gene50578:intron | 20.0% |
| ! | GATAAATATGAGAGACTATT+GGG | - | chr1.2:54114799-54114818 | None:intergenic | 25.0% |
| ! | TGATAAATATGAGAGACTAT+TGG | - | chr1.2:54114800-54114819 | None:intergenic | 25.0% |
| !!! | ATTTGTCGATTTTTTTTCTC+GGG | + | chr1.2:54114984-54115003 | MS.gene50578:intron | 25.0% |
| !!! | GAATTTTATTTGTACTTCTG+TGG | + | chr1.2:54114744-54114763 | MS.gene50578:intron | 25.0% |
| !!! | GTTTTTGATTTTGTCAAACT+CGG | + | chr1.2:54114889-54114908 | MS.gene50578:intron | 25.0% |
| AATCGAGCCATAGTATAAAA+TGG | - | chr1.2:54115068-54115087 | None:intergenic | 30.0% | |
| CAAAATCAAAAACCACATGT+TGG | - | chr1.2:54114882-54114901 | None:intergenic | 30.0% | |
| TCATCATTACAAAATTGGGA+GGG | - | chr1.2:54114958-54114977 | None:intergenic | 30.0% | |
| TTAGTGCATGTTTGATGTTA+CGG | + | chr1.2:54114836-54114855 | MS.gene50578:intron | 30.0% | |
| TTCATCATTACAAAATTGGG+AGG | - | chr1.2:54114959-54114978 | None:intergenic | 30.0% | |
| ! | ATACTATGGCTCGATTATTT+TGG | + | chr1.2:54115072-54115091 | MS.gene50578:intron | 30.0% |
| !! | TCTACTCCATTTTTAATCGA+CGG | + | chr1.2:54115213-54115232 | MS.gene50578:intron | 30.0% |
| !!! | TTATTTTGGCAATTCGATCA+CGG | + | chr1.2:54115086-54115105 | MS.gene50578:intron | 30.0% |
| !!! | TTTGTCGATTTTTTTTCTCG+GGG | + | chr1.2:54114985-54115004 | MS.gene50578:intron | 30.0% |
| AAGAACAACATACCAAGAAC+AGG | - | chr1.2:54114637-54114656 | None:intergenic | 35.0% | |
| AAGCAACTCAAACATTGGAA+TGG | + | chr1.2:54115356-54115375 | MS.gene50578:CDS | 35.0% | |
| AGCAACTCAAACATTGGAAT+GGG | + | chr1.2:54115357-54115376 | MS.gene50578:CDS | 35.0% | |
| AGTGAAAGCAACTCAAACAT+TGG | + | chr1.2:54115351-54115370 | MS.gene50578:CDS | 35.0% | |
| ATTGTTGAAGATCCAACATG+TGG | + | chr1.2:54114867-54114886 | MS.gene50578:intron | 35.0% | |
| TGTCTCGCTTGTTTGATATT+TGG | + | chr1.2:54115156-54115175 | MS.gene50578:intron | 35.0% | |
| TTATTCAGGATTGCCAACAA+TGG | + | chr1.2:54115278-54115297 | MS.gene50578:intron | 35.0% | |
| ! | AAACCTCCGTCGATTAAAAA+TGG | - | chr1.2:54115222-54115241 | None:intergenic | 35.0% |
| ! | ATTTTTAATCGACGGAGGTT+TGG | + | chr1.2:54115221-54115240 | MS.gene50578:intron | 35.0% |
| CAAAAAGGATCCAACCAAGA+AGG | - | chr1.2:54115500-54115519 | None:intergenic | 40.0% | |
| CACCTTCAATTGCTTGAATC+AGG | - | chr1.2:54115118-54115137 | None:intergenic | 40.0% | |
| CTGATTCAAGCAATTGAAGG+TGG | + | chr1.2:54115116-54115135 | MS.gene50578:intron | 40.0% | |
| GTCCTGATTCAAGCAATTGA+AGG | + | chr1.2:54115113-54115132 | MS.gene50578:intron | 40.0% | |
| TAAGACCGAACACACCAAAA+AGG | - | chr1.2:54115515-54115534 | None:intergenic | 40.0% | |
| TTGCTTTCACTCACAGAAGA+AGG | - | chr1.2:54115342-54115361 | None:intergenic | 40.0% | |
| ! | CTTCTTGGTTGGATCCTTTT+TGG | + | chr1.2:54115498-54115517 | MS.gene50578:CDS | 40.0% |
| ! | TCTTTTGTGCAAGACATGCA+CGG | - | chr1.2:54114591-54114610 | None:intergenic | 40.0% |
| !! | ACTCCATTTTTAATCGACGG+AGG | + | chr1.2:54115216-54115235 | MS.gene50578:intron | 40.0% |
| !! | GATGAAAGAATGAGCACTGA+AGG | + | chr1.2:54115444-54115463 | MS.gene50578:CDS | 40.0% |
| !! | TGGATCCTTTTTGGTGTGTT+CGG | + | chr1.2:54115507-54115526 | MS.gene50578:CDS | 40.0% |
| AAGGTTGTTGCTGAGTCCAA+AGG | - | chr1.2:54115481-54115500 | None:intergenic | 45.0% | |
| AGGATTGCCAACAATGGCTA+AGG | + | chr1.2:54115284-54115303 | MS.gene50578:intron | 45.0% | |
| AGGTTGTTGCTGAGTCCAAA+GGG | - | chr1.2:54115480-54115499 | None:intergenic | 45.0% | |
| AGTTGTAGTGGTAGCTGAGA+TGG | - | chr1.2:54114557-54114576 | None:intergenic | 45.0% | |
| ATTGCCAACAATGGCTAAGG+TGG | + | chr1.2:54115287-54115306 | MS.gene50578:CDS | 45.0% | |
| CAAAATTGGGAGGGTAGTCT+TGG | - | chr1.2:54114949-54114968 | None:intergenic | 45.0% | |
| CAACATACCAAGAACAGGAG+AGG | - | chr1.2:54114632-54114651 | None:intergenic | 45.0% | |
| CTTGAAGAGGATGAAGAGTC+TGG | + | chr1.2:54115567-54115586 | MS.gene50578:CDS | 45.0% | |
| GTTTCATCCTCTCCTGTTCT+TGG | + | chr1.2:54114622-54114641 | MS.gene50578:CDS | 45.0% | |
| TCAGCAACAACCTTCTTGGT+TGG | + | chr1.2:54115487-54115506 | MS.gene50578:CDS | 45.0% | |
| TCTTTCATCCACCAATGCCA+TGG | - | chr1.2:54115433-54115452 | None:intergenic | 45.0% | |
| TTCATCCTCTTCAAGACTCG+AGG | - | chr1.2:54115562-54115581 | None:intergenic | 45.0% | |
| TTGCCAACAATGGCTAAGGT+GGG | + | chr1.2:54115288-54115307 | MS.gene50578:CDS | 45.0% | |
| ! | CTTTTGTGCAAGACATGCAC+GGG | - | chr1.2:54114590-54114609 | None:intergenic | 45.0% |
| ! | TGGTGTGTTCGGTCTTATCT+GGG | + | chr1.2:54115518-54115537 | MS.gene50578:CDS | 45.0% |
| ! | TTGGTGTGTTCGGTCTTATC+TGG | + | chr1.2:54115517-54115536 | MS.gene50578:CDS | 45.0% |
| !! | AGAATGAGCACTGAAGGAAC+CGG | + | chr1.2:54115450-54115469 | MS.gene50578:CDS | 45.0% |
| CTTCCCACCTTAGCCATTGT+TGG | - | chr1.2:54115294-54115313 | None:intergenic | 50.0% | |
| GGACTCAGCAACAACCTTCT+TGG | + | chr1.2:54115483-54115502 | MS.gene50578:CDS | 50.0% | |
| GGAGAGGATGAAACTGCAAG+AGG | - | chr1.2:54114616-54114635 | None:intergenic | 50.0% | |
| GGTGATCGATGCAGTTGTAG+TGG | - | chr1.2:54114569-54114588 | None:intergenic | 50.0% | |
| ! | AAGAGTGAGGTGTTCGATGG+AGG | + | chr1.2:54115311-54115330 | MS.gene50578:CDS | 50.0% |
| ! | AGAGTGAGGTGTTCGATGGA+GGG | + | chr1.2:54115312-54115331 | MS.gene50578:CDS | 50.0% |
| !! | GAATGAGCACTGAAGGAACC+GGG | + | chr1.2:54115451-54115470 | MS.gene50578:CDS | 50.0% |
| CTACGCCTCGAGTCTTGAAG+AGG | + | chr1.2:54115554-54115573 | MS.gene50578:CDS | 55.0% | |
| TATGTCAAGCCCAGCAGCCA+TGG | + | chr1.2:54115413-54115432 | MS.gene50578:CDS | 55.0% | |
| TGGCTAAGGTGGGAAGAGTG+AGG | + | chr1.2:54115298-54115317 | MS.gene50578:CDS | 55.0% | |
| ! | GGGAAGAGTGAGGTGTTCGA+TGG | + | chr1.2:54115308-54115327 | MS.gene50578:CDS | 55.0% |
| AAGCCCAGCAGCCATGGCAT+TGG | + | chr1.2:54115419-54115438 | MS.gene50578:CDS | 60.0% | |
| CCACCAATGCCATGGCTGCT+GGG | - | chr1.2:54115425-54115444 | None:intergenic | 60.0% | |
| GAAGGAACCGGGCTTCCCTT+TGG | + | chr1.2:54115462-54115481 | MS.gene50578:CDS | 60.0% | |
| GCTGAGTCCAAAGGGAAGCC+CGG | - | chr1.2:54115472-54115491 | None:intergenic | 60.0% | |
| TCCACCAATGCCATGGCTGC+TGG | - | chr1.2:54115426-54115445 | None:intergenic | 60.0% | |
| ! | CCCAGCAGCCATGGCATTGG+TGG | + | chr1.2:54115422-54115441 | MS.gene50578:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 54114547 | 54115602 | 54114547 | ID=MS.gene50578 |
| chr1.2 | mRNA | 54114547 | 54115602 | 54114547 | ID=MS.gene50578.t1;Parent=MS.gene50578 |
| chr1.2 | exon | 54114547 | 54114643 | 54114547 | ID=MS.gene50578.t1.exon1;Parent=MS.gene50578.t1 |
| chr1.2 | CDS | 54114547 | 54114643 | 54114547 | ID=cds.MS.gene50578.t1;Parent=MS.gene50578.t1 |
| chr1.2 | exon | 54115286 | 54115602 | 54115286 | ID=MS.gene50578.t1.exon2;Parent=MS.gene50578.t1 |
| chr1.2 | CDS | 54115286 | 54115602 | 54115286 | ID=cds.MS.gene50578.t1;Parent=MS.gene50578.t1 |
| Gene Sequence |
| Protein sequence |
