AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50734


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50734.t1 MTR_4g071150 98.026 152 1 1 1 150 1 152 9.78e-101 285
MS.gene50734.t1 MTR_2g096610 87.333 150 17 1 1 150 1 148 4.41e-83 241
MS.gene50734.t1 MTR_4g064005 94.000 150 7 1 1 150 1 148 1.32e-82 239
MS.gene50734.t1 MTR_4g063410 94.000 150 7 1 1 150 1 148 2.70e-82 239
MS.gene50734.t1 MTR_2g096570 91.558 154 8 2 1 150 1 153 5.64e-82 238
MS.gene50734.t1 MTR_4g063280 92.424 132 9 1 20 150 14 145 2.84e-81 236
MS.gene50734.t1 MTR_4g064967 93.333 150 8 1 1 150 1 148 2.98e-80 234
MS.gene50734.t1 MTR_8g086640 96.212 132 5 0 1 132 1 132 4.27e-75 221
MS.gene50734.t1 MTR_7g113160 79.699 133 26 1 10 142 13 144 5.01e-71 210
MS.gene50734.t1 MTR_7g108320 75.000 128 26 3 4 130 6 128 7.58e-60 182
MS.gene50734.t1 MTR_7g114060 74.219 128 29 1 3 130 2 125 7.85e-60 182
MS.gene50734.t1 MTR_7g114040 74.219 128 29 1 3 130 2 125 7.85e-60 182
MS.gene50734.t1 MTR_7g093910 73.228 127 31 1 4 130 5 128 9.33e-60 181
MS.gene50734.t1 MTR_2g082510 70.543 129 38 0 11 139 3 131 3.21e-59 180
MS.gene50734.t1 MTR_2g082220 73.950 119 31 0 11 129 3 121 1.65e-57 176
MS.gene50734.t1 MTR_6g005150 62.992 127 33 3 24 149 26 139 3.16e-47 150
MS.gene50734.t1 MTR_4g063300 75.238 105 21 1 45 149 10 109 3.34e-46 146
MS.gene50734.t1 MTR_7g114050 68.644 118 22 3 17 130 10 116 2.19e-45 145
MS.gene50734.t1 MTR_1g011750 60.714 112 42 2 21 131 23 133 3.59e-36 121
MS.gene50734.t1 MTR_1g011850 60.714 112 42 2 21 131 23 133 3.59e-36 121
MS.gene50734.t1 MTR_1g114050 60.550 109 41 2 21 128 20 127 2.90e-35 119
MS.gene50734.t1 MTR_1g071040 62.500 104 37 2 26 128 28 130 1.54e-34 117
MS.gene50734.t1 MTR_1g069770 48.958 96 48 1 45 139 9 104 5.64e-25 92.4
MS.gene50734.t1 MTR_4g048490 44.762 105 29 2 24 128 26 101 1.70e-16 70.9
MS.gene50734.t1 MTR_7g010440 70.588 51 15 0 78 128 122 172 2.24e-16 74.3
MS.gene50734.t1 MTR_4g005910 74.419 43 11 0 87 129 4 46 3.21e-16 68.6
MS.gene50734.t1 MTR_7g027610 41.525 118 42 4 20 135 10 102 5.62e-15 67.0
MS.gene50734.t1 MTR_0653s0030 54.545 55 25 0 55 109 43 97 4.58e-12 60.5
MS.gene50734.t1 MTR_2g096590 70.000 40 12 0 110 149 12 51 5.34e-12 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50734.t1 AT5G02560 79.739 153 28 1 1 150 1 153 1.48e-77 227
MS.gene50734.t1 AT5G27670 76.316 152 32 3 1 150 1 150 3.32e-74 218
MS.gene50734.t1 AT5G02560 68.927 177 28 2 1 150 1 177 1.06e-72 216
MS.gene50734.t1 AT5G59870 75.000 148 36 1 4 150 3 150 8.98e-72 212
MS.gene50734.t1 AT1G51060 76.667 120 28 0 11 130 3 122 9.15e-61 184
MS.gene50734.t1 AT4G27230 71.538 130 32 1 11 140 3 127 1.43e-59 181
MS.gene50734.t1 AT4G27230 71.538 130 32 1 11 140 3 127 1.43e-59 181
MS.gene50734.t1 AT3G20670 75.630 119 29 0 11 129 3 121 4.77e-59 179
MS.gene50734.t1 AT1G08880 71.654 127 33 1 7 130 2 128 3.47e-58 177
MS.gene50734.t1 AT5G54640 74.790 119 30 0 11 129 3 121 4.51e-58 177
MS.gene50734.t1 AT1G54690 72.868 129 29 3 3 130 5 128 1.19e-57 176
MS.gene50734.t1 AT2G38810 60.714 112 42 2 21 131 25 135 6.97e-37 123
MS.gene50734.t1 AT2G38810 60.714 112 42 2 21 131 25 135 6.97e-37 123
MS.gene50734.t1 AT2G38810 60.714 112 42 2 21 131 25 135 6.97e-37 123
MS.gene50734.t1 AT2G38810 60.714 112 42 2 21 131 25 135 6.97e-37 123
MS.gene50734.t1 AT1G52740 62.857 105 37 2 25 128 27 130 6.18e-35 118
MS.gene50734.t1 AT3G54560 60.185 108 41 2 25 131 29 135 1.25e-34 118
MS.gene50734.t1 AT3G54560 60.185 108 41 2 25 131 29 135 1.25e-34 118
MS.gene50734.t1 AT4G13570 48.913 92 45 2 32 122 28 118 1.97e-20 81.3
MS.gene50734.t1 AT4G13570 48.352 91 45 2 33 122 35 124 5.65e-20 80.1

Find 56 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGACTTTCCAGCCTTCTT+TGG 0.241789 4.3:+34135800 None:intergenic
AATCAGATCTAAGCCTTCTT+TGG 0.242120 4.3:+34135779 None:intergenic
GTCTTCTTAGGCAACAGAAT+TGG 0.279189 4.3:+34135851 None:intergenic
TTAGCCCTTTCAGTCTTCTT+AGG 0.279305 4.3:+34135839 None:intergenic
CGATTCTTCCGACTGGGAAT+TGG 0.285182 4.3:+34136119 None:intergenic
CATTCCAAGGCATTTGTTGC+TGG 0.294047 4.3:-34135954 MS.gene50734:CDS
CTTGTCACCGACTTCTTCCT+TGG 0.303917 4.3:+34136160 None:intergenic
GGCTCCCTTCTTTGTTGTCT+TGG 0.304409 4.3:+34136204 None:intergenic
GAGCATATCGACCTTTCTTC+AGG 0.357439 4.3:+34136089 None:intergenic
TGCTGCTGAGGTTCTTGAAT+TGG 0.361111 4.3:-34136014 MS.gene50734:CDS
GAGCTTGGGAAGTTGCTTGC+TGG 0.378028 4.3:-34135914 MS.gene50734:CDS
GGCATTTGTTGCTGGCTGTT+AGG 0.378218 4.3:-34135946 MS.gene50734:CDS
TCTTCTTAGGCAACAGAATT+GGG 0.399065 4.3:+34135852 None:intergenic
GAAGGGAGCCGGTGGAAGAA+AGG 0.417242 4.3:-34136191 MS.gene50734:CDS
AAGGGAGCCGGTGGAAGAAA+GGG 0.429527 4.3:-34136190 MS.gene50734:CDS
CAATTCCCAGTCGGAAGAAT+CGG 0.431861 4.3:-34136118 MS.gene50734:CDS
AACAGAATTGGGTTAATGTT+CGG 0.436254 4.3:+34135863 None:intergenic
AGAACTGCTGCTAGGTAAAC+CGG 0.439504 4.3:+34136046 None:intergenic
ACCTCCTCCCTTTCTTCCAC+CGG 0.471747 4.3:+34136183 None:intergenic
GCGTGTCGGCACCGGTGCTC+CGG 0.486643 4.3:-34136065 MS.gene50734:CDS
GAATTGGAGACCGGCTCTGA+TGG 0.486869 4.3:+34136135 None:intergenic
TATGCTCAGCGTGTCGGCAC+CGG 0.489995 4.3:-34136073 MS.gene50734:CDS
TCTTGAATATCTTGCTGCTG+AGG 0.496391 4.3:-34136026 MS.gene50734:CDS
AAGCACCAAGACAACAAAGA+AGG 0.501816 4.3:-34136209 MS.gene50734:CDS
GTTAGGAATGATGAAGAGCT+TGG 0.506747 4.3:-34135929 MS.gene50734:CDS
AGCAAGGAACCAAAGAAGGC+TGG 0.510603 4.3:-34135809 MS.gene50734:CDS
GCTGAGGTTCTTGAATTGGC+AGG 0.519701 4.3:-34136010 MS.gene50734:CDS
CTCGTGATAACAAGAAGAAT+AGG 0.522113 4.3:-34135979 MS.gene50734:CDS
TTAGGAATGATGAAGAGCTT+GGG 0.524474 4.3:-34135928 MS.gene50734:CDS
GGCTGGAAAGTCTCCAAAGA+AGG 0.525629 4.3:-34135792 MS.gene50734:CDS
ACAGCCAGCAACAAATGCCT+TGG 0.526781 4.3:+34135950 None:intergenic
TGCTAGGTAAACCGGAGCAC+CGG 0.537567 4.3:+34136054 None:intergenic
ATCGGTCGATACCTGAAGAA+AGG 0.548682 4.3:-34136100 MS.gene50734:CDS
TATCGACCGATTCTTCCGAC+TGG 0.549955 4.3:+34136112 None:intergenic
TGGTAGCAAGGAACCAAAGA+AGG 0.550474 4.3:-34135813 MS.gene50734:CDS
TGTTGCCTAAGAAGACTGAA+AGG 0.556495 4.3:-34135844 MS.gene50734:CDS
AGCACCAAGACAACAAAGAA+GGG 0.558080 4.3:-34136208 MS.gene50734:CDS
GAAGAAAGGGAGGAGGTCCA+AGG 0.558674 4.3:-34136177 MS.gene50734:CDS
TCCGACTGGGAATTGGAGAC+CGG 0.563841 4.3:+34136126 None:intergenic
AAGAACACCACCATGAGCTA+TGG 0.573393 4.3:+34135886 None:intergenic
AAGACAACAAAGAAGGGAGC+CGG 0.578427 4.3:-34136202 MS.gene50734:CDS
GATATTCAAGAACTGCTGCT+AGG 0.583613 4.3:+34136038 None:intergenic
GCTGGTGTTACCATAGCTCA+TGG 0.586669 4.3:-34135896 MS.gene50734:CDS
GTGACAAGATCCATCAGAGC+CGG 0.589197 4.3:-34136145 MS.gene50734:CDS
GTTGCCTAAGAAGACTGAAA+GGG 0.589268 4.3:-34135843 MS.gene50734:CDS
GCCGGTGGAAGAAAGGGAGG+AGG 0.593863 4.3:-34136184 MS.gene50734:CDS
AAGGGCTAACACTGGTAGCA+AGG 0.594830 4.3:-34135825 MS.gene50734:CDS
ACAACAAAGAAGGGAGCCGG+TGG 0.595449 4.3:-34136199 MS.gene50734:CDS
GGAGCCGGTGGAAGAAAGGG+AGG 0.604037 4.3:-34136187 MS.gene50734:CDS
GGTCGATATGCTCAGCGTGT+CGG 0.611319 4.3:-34136079 MS.gene50734:CDS
ATCGACCGATTCTTCCGACT+GGG 0.613102 4.3:+34136113 None:intergenic
AGGAGGTCCAAGGAAGAAGT+CGG 0.620460 4.3:-34136167 MS.gene50734:CDS
GCCGGTCTCCAATTCCCAGT+CGG 0.630576 4.3:-34136127 MS.gene50734:CDS
AGAAGAATAGGATCATTCCA+AGG 0.652341 4.3:-34135967 MS.gene50734:CDS
AAGACTGAAAGGGCTAACAC+TGG 0.665786 4.3:-34135833 MS.gene50734:CDS
GGTGTTACCATAGCTCATGG+TGG 0.742529 4.3:-34135893 MS.gene50734:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACAGAATTGGGTTAATGTT+CGG + chr4.3:34136144-34136163 None:intergenic 30.0%
AGAAGAATAGGATCATTCCA+AGG - chr4.3:34136037-34136056 MS.gene50734:CDS 35.0%
CTCGTGATAACAAGAAGAAT+AGG - chr4.3:34136025-34136044 MS.gene50734:CDS 35.0%
TCTTCTTAGGCAACAGAATT+GGG + chr4.3:34136155-34136174 None:intergenic 35.0%
TTAGGAATGATGAAGAGCTT+GGG - chr4.3:34136076-34136095 MS.gene50734:CDS 35.0%
AAGCACCAAGACAACAAAGA+AGG - chr4.3:34135795-34135814 MS.gene50734:CDS 40.0%
AGCACCAAGACAACAAAGAA+GGG - chr4.3:34135796-34135815 MS.gene50734:CDS 40.0%
GATATTCAAGAACTGCTGCT+AGG + chr4.3:34135969-34135988 None:intergenic 40.0%
GTCTTCTTAGGCAACAGAAT+TGG + chr4.3:34136156-34136175 None:intergenic 40.0%
GTTAGGAATGATGAAGAGCT+TGG - chr4.3:34136075-34136094 MS.gene50734:CDS 40.0%
GTTGCCTAAGAAGACTGAAA+GGG - chr4.3:34136161-34136180 MS.gene50734:CDS 40.0%
TCTTGAATATCTTGCTGCTG+AGG - chr4.3:34135978-34135997 MS.gene50734:CDS 40.0%
TGTTGCCTAAGAAGACTGAA+AGG - chr4.3:34136160-34136179 MS.gene50734:CDS 40.0%
TTAGCCCTTTCAGTCTTCTT+AGG + chr4.3:34136168-34136187 None:intergenic 40.0%
AAGAACACCACCATGAGCTA+TGG + chr4.3:34136121-34136140 None:intergenic 45.0%
AAGACAACAAAGAAGGGAGC+CGG - chr4.3:34135802-34135821 MS.gene50734:CDS 45.0%
AAGACTGAAAGGGCTAACAC+TGG - chr4.3:34136171-34136190 MS.gene50734:CDS 45.0%
AGAACTGCTGCTAGGTAAAC+CGG + chr4.3:34135961-34135980 None:intergenic 45.0%
ATCGGTCGATACCTGAAGAA+AGG - chr4.3:34135904-34135923 MS.gene50734:CDS 45.0%
CAATTCCCAGTCGGAAGAAT+CGG - chr4.3:34135886-34135905 MS.gene50734:CDS 45.0%
GAGCATATCGACCTTTCTTC+AGG + chr4.3:34135918-34135937 None:intergenic 45.0%
TGCTGCTGAGGTTCTTGAAT+TGG - chr4.3:34135990-34136009 MS.gene50734:CDS 45.0%
TGGTAGCAAGGAACCAAAGA+AGG - chr4.3:34136191-34136210 MS.gene50734:CDS 45.0%
! CATTCCAAGGCATTTGTTGC+TGG - chr4.3:34136050-34136069 MS.gene50734:CDS 45.0%
AAGGGCTAACACTGGTAGCA+AGG - chr4.3:34136179-34136198 MS.gene50734:CDS 50.0%
ACAGCCAGCAACAAATGCCT+TGG + chr4.3:34136057-34136076 None:intergenic 50.0%
AGCAAGGAACCAAAGAAGGC+TGG - chr4.3:34136195-34136214 MS.gene50734:CDS 50.0%
AGGAGGTCCAAGGAAGAAGT+CGG - chr4.3:34135837-34135856 MS.gene50734:CDS 50.0%
CGATTCTTCCGACTGGGAAT+TGG + chr4.3:34135888-34135907 None:intergenic 50.0%
GCTGAGGTTCTTGAATTGGC+AGG - chr4.3:34135994-34136013 MS.gene50734:CDS 50.0%
GGAGACTTTCCAGCCTTCTT+TGG + chr4.3:34136207-34136226 None:intergenic 50.0%
GGCTGGAAAGTCTCCAAAGA+AGG - chr4.3:34136212-34136231 MS.gene50734:CDS 50.0%
GTGACAAGATCCATCAGAGC+CGG - chr4.3:34135859-34135878 MS.gene50734:CDS 50.0%
! ATCGACCGATTCTTCCGACT+GGG + chr4.3:34135894-34135913 None:intergenic 50.0%
! GGCATTTGTTGCTGGCTGTT+AGG - chr4.3:34136058-34136077 MS.gene50734:CDS 50.0%
! GGCTCCCTTCTTTGTTGTCT+TGG + chr4.3:34135803-34135822 None:intergenic 50.0%
! GGTGTTACCATAGCTCATGG+TGG - chr4.3:34136111-34136130 MS.gene50734:CDS 50.0%
! TATCGACCGATTCTTCCGAC+TGG + chr4.3:34135895-34135914 None:intergenic 50.0%
!! CTTGTCACCGACTTCTTCCT+TGG + chr4.3:34135847-34135866 None:intergenic 50.0%
!! GCTGGTGTTACCATAGCTCA+TGG - chr4.3:34136108-34136127 MS.gene50734:CDS 50.0%
AAGGGAGCCGGTGGAAGAAA+GGG - chr4.3:34135814-34135833 MS.gene50734:CDS 55.0%
ACAACAAAGAAGGGAGCCGG+TGG - chr4.3:34135805-34135824 MS.gene50734:CDS 55.0%
ACCTCCTCCCTTTCTTCCAC+CGG + chr4.3:34135824-34135843 None:intergenic 55.0%
GAAGAAAGGGAGGAGGTCCA+AGG - chr4.3:34135827-34135846 MS.gene50734:CDS 55.0%
GAGCTTGGGAAGTTGCTTGC+TGG - chr4.3:34136090-34136109 MS.gene50734:CDS 55.0%
GGTCGATATGCTCAGCGTGT+CGG - chr4.3:34135925-34135944 MS.gene50734:CDS 55.0%
TCCGACTGGGAATTGGAGAC+CGG + chr4.3:34135881-34135900 None:intergenic 55.0%
TGCTAGGTAAACCGGAGCAC+CGG + chr4.3:34135953-34135972 None:intergenic 55.0%
! GAATTGGAGACCGGCTCTGA+TGG + chr4.3:34135872-34135891 None:intergenic 55.0%
GAAGGGAGCCGGTGGAAGAA+AGG - chr4.3:34135813-34135832 MS.gene50734:CDS 60.0%
GCCGGTCTCCAATTCCCAGT+CGG - chr4.3:34135877-34135896 MS.gene50734:CDS 60.0%
! TATGCTCAGCGTGTCGGCAC+CGG - chr4.3:34135931-34135950 MS.gene50734:CDS 60.0%
GCCGGTGGAAGAAAGGGAGG+AGG - chr4.3:34135820-34135839 MS.gene50734:CDS 65.0%
GGAGCCGGTGGAAGAAAGGG+AGG - chr4.3:34135817-34135836 MS.gene50734:CDS 65.0%
!! GCGTGTCGGCACCGGTGCTC+CGG - chr4.3:34135939-34135958 MS.gene50734:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 34135787 34136239 34135787 ID=MS.gene50734
chr4.3 mRNA 34135787 34136239 34135787 ID=MS.gene50734.t1;Parent=MS.gene50734
chr4.3 exon 34135787 34136239 34135787 ID=MS.gene50734.t1.exon1;Parent=MS.gene50734.t1
chr4.3 CDS 34135787 34136239 34135787 ID=cds.MS.gene50734.t1;Parent=MS.gene50734.t1
Gene Sequence

>MS.gene50734

ATGGACGCAAGCACCAAGACAACAAAGAAGGGAGCCGGTGGAAGAAAGGGAGGAGGTCCAAGGAAGAAGTCGGTGACAAGATCCATCAGAGCCGGTCTCCAATTCCCAGTCGGAAGAATCGGTCGATACCTGAAGAAAGGTCGATATGCTCAGCGTGTCGGCACCGGTGCTCCGGTTTACCTAGCAGCAGTTCTTGAATATCTTGCTGCTGAGGTTCTTGAATTGGCAGGAAACGCTGCTCGTGATAACAAGAAGAATAGGATCATTCCAAGGCATTTGTTGCTGGCTGTTAGGAATGATGAAGAGCTTGGGAAGTTGCTTGCTGGTGTTACCATAGCTCATGGTGGTGTTCTTCCGAACATTAACCCAATTCTGTTGCCTAAGAAGACTGAAAGGGCTAACACTGGTAGCAAGGAACCAAAGAAGGCTGGAAAGTCTCCAAAGAAGGCTTAG

Protein sequence

>MS.gene50734.t1

MDASTKTTKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHLLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKTERANTGSKEPKKAGKSPKKA