Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51711.t1 | XP_003598367.2 | 96.6 | 436 | 15 | 0 | 1 | 436 | 1 | 436 | 2.50E-241 | 844.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51711.t1 | Q3EAQ5 | 58.1 | 442 | 172 | 4 | 1 | 436 | 1 | 435 | 3.8e-142 | 506.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51711.t1 | G7IVI0 | 96.6 | 436 | 15 | 0 | 1 | 436 | 1 | 436 | 1.8e-241 | 844.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51711.t1 | MTR_3g010820 | 96.560 | 436 | 15 | 0 | 1 | 436 | 1 | 436 | 0.0 | 867 |
| MS.gene51711.t1 | MTR_8g062990 | 94.495 | 436 | 24 | 0 | 1 | 436 | 1 | 436 | 0.0 | 845 |
| MS.gene51711.t1 | MTR_8g042770 | 69.794 | 437 | 130 | 2 | 1 | 436 | 1 | 436 | 0.0 | 654 |
| MS.gene51711.t1 | MTR_8g042780 | 70.726 | 427 | 121 | 1 | 10 | 436 | 7 | 429 | 0.0 | 652 |
| MS.gene51711.t1 | MTR_8g042780 | 70.726 | 427 | 121 | 1 | 10 | 436 | 7 | 429 | 0.0 | 652 |
| MS.gene51711.t1 | MTR_3g086440 | 72.706 | 436 | 118 | 1 | 1 | 436 | 1 | 435 | 0.0 | 642 |
| MS.gene51711.t1 | MTR_8g042870 | 68.421 | 437 | 133 | 2 | 1 | 436 | 1 | 433 | 0.0 | 624 |
| MS.gene51711.t1 | MTR_3g010850 | 70.938 | 437 | 120 | 4 | 1 | 436 | 1 | 431 | 0.0 | 604 |
| MS.gene51711.t1 | MTR_3g010850 | 70.000 | 390 | 113 | 2 | 47 | 436 | 34 | 419 | 0.0 | 530 |
| MS.gene51711.t1 | MTR_8g042820 | 70.070 | 284 | 81 | 1 | 153 | 436 | 34 | 313 | 7.17e-148 | 423 |
| MS.gene51711.t1 | MTR_1194s0010 | 69.231 | 143 | 44 | 0 | 10 | 152 | 7 | 149 | 3.22e-70 | 219 |
| MS.gene51711.t1 | MTR_1157s0010 | 69.725 | 109 | 33 | 0 | 328 | 436 | 1 | 109 | 2.70e-47 | 160 |
| MS.gene51711.t1 | MTR_8g075280 | 47.872 | 94 | 32 | 3 | 42 | 134 | 107 | 184 | 4.43e-14 | 71.6 |
| MS.gene51711.t1 | MTR_8g075280 | 47.872 | 94 | 32 | 3 | 42 | 134 | 107 | 184 | 1.65e-13 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51711.t1 | AT3G43790 | 58.145 | 442 | 172 | 4 | 1 | 436 | 1 | 435 | 0.0 | 520 |
| MS.gene51711.t1 | AT3G43790 | 58.145 | 442 | 172 | 4 | 1 | 436 | 1 | 435 | 0.0 | 520 |
| MS.gene51711.t1 | AT3G43790 | 58.145 | 442 | 172 | 4 | 1 | 436 | 1 | 435 | 0.0 | 519 |
| MS.gene51711.t1 | AT3G43790 | 58.145 | 442 | 172 | 4 | 1 | 436 | 1 | 435 | 0.0 | 519 |
| MS.gene51711.t1 | AT5G13750 | 56.264 | 439 | 173 | 3 | 7 | 436 | 6 | 434 | 0.0 | 516 |
| MS.gene51711.t1 | AT5G13740 | 58.161 | 435 | 173 | 4 | 8 | 434 | 7 | 440 | 1.14e-179 | 509 |
| MS.gene51711.t1 | AT5G13740 | 58.161 | 435 | 173 | 4 | 8 | 434 | 7 | 440 | 6.92e-179 | 508 |
| MS.gene51711.t1 | AT3G43790 | 57.282 | 412 | 163 | 4 | 1 | 406 | 1 | 405 | 2.09e-166 | 474 |
| MS.gene51711.t1 | AT5G13750 | 54.567 | 416 | 170 | 3 | 7 | 413 | 6 | 411 | 2.19e-166 | 474 |
| MS.gene51711.t1 | AT5G13750 | 54.567 | 416 | 170 | 3 | 7 | 413 | 6 | 411 | 2.19e-166 | 474 |
| MS.gene51711.t1 | AT5G13750 | 55.866 | 358 | 139 | 3 | 88 | 436 | 1 | 348 | 6.37e-141 | 409 |
| MS.gene51711.t1 | AT5G13940 | 45.946 | 74 | 24 | 1 | 275 | 348 | 11 | 68 | 3.20e-13 | 72.0 |
| MS.gene51711.t1 | AT5G13940 | 45.946 | 74 | 24 | 1 | 275 | 348 | 162 | 219 | 3.68e-13 | 72.0 |
Find 100 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCTTCATAGCAATGCTTTC+TGG | 0.080739 | 3.4:+6827338 | MS.gene51711:CDS |
| CTTAATAGATCTTCTCATTA+TGG | 0.133209 | 3.4:+6826742 | MS.gene51711:intron |
| ACCAGCTTTGGGAGGTTATT+TGG | 0.181938 | 3.4:+6820479 | MS.gene51711:CDS |
| TGGGGCATGGGTTTAATAAT+TGG | 0.228616 | 3.4:+6820456 | MS.gene51711:CDS |
| TTTAATAATTGGACCAGCTT+TGG | 0.269939 | 3.4:+6820467 | MS.gene51711:CDS |
| GAATCCTTTGGAAATAGATT+TGG | 0.276086 | 3.4:-6820803 | None:intergenic |
| TTAATAGATCTTCTCATTAT+GGG | 0.296541 | 3.4:+6826743 | MS.gene51711:intron |
| CACAACAATAGATGACATTA+AGG | 0.296857 | 3.4:-6821544 | None:intergenic |
| CTTTCCATACCTATATTTCA+TGG | 0.306308 | 3.4:+6819083 | MS.gene51711:CDS |
| CGGTAGTAATTGCCTGCATC+TGG | 0.308405 | 3.4:+6820998 | MS.gene51711:CDS |
| AAAATGGAAGATGGAAATAT+TGG | 0.310584 | 3.4:+6818689 | None:intergenic |
| AAGAAATAAACCAGTGATTA+TGG | 0.311778 | 3.4:-6827691 | None:intergenic |
| GAAATGGAAGGCAAGAAATA+TGG | 0.321659 | 3.4:-6820955 | None:intergenic |
| TTCGAAATCTCTTGAACATA+TGG | 0.324637 | 3.4:+6818798 | MS.gene51711:CDS |
| TTCAAACCACCTAACCTTCT+AGG | 0.336667 | 3.4:-6826774 | None:intergenic |
| AATCCTTTGGAAATAGATTT+GGG | 0.340594 | 3.4:-6820802 | None:intergenic |
| TATTTCCAAAGGATTCCTTC+TGG | 0.344595 | 3.4:+6820810 | MS.gene51711:CDS |
| GCTTTAGAAACTGGAAGAAA+TGG | 0.345551 | 3.4:+6821465 | MS.gene51711:CDS |
| GTAAGATTTGCTCGCATCTC+CGG | 0.354320 | 3.4:+6827193 | MS.gene51711:CDS |
| TTCAGTGCAGCTTGGGGCAT+GGG | 0.362285 | 3.4:+6820444 | MS.gene51711:CDS |
| CGAGAAGAAAAGCAAGCTCT+TGG | 0.364652 | 3.4:+6820110 | MS.gene51711:CDS |
| GAGATGCGAGCAAATCTTAC+AGG | 0.370847 | 3.4:-6827190 | None:intergenic |
| GGTGATGTTCTTGCAATATC+AGG | 0.378966 | 3.4:+6826816 | MS.gene51711:CDS |
| TTAATAATTGGACCAGCTTT+GGG | 0.379847 | 3.4:+6820468 | MS.gene51711:CDS |
| ATGAAAACCTCTTCCTAAAC+TGG | 0.393957 | 3.4:+6821520 | MS.gene51711:CDS |
| ATTTCAGTTCAGTGCAGCTT+GGG | 0.399921 | 3.4:+6820437 | MS.gene51711:intron |
| AATTGCCTGCATCTGGCTTC+CGG | 0.400402 | 3.4:+6821005 | MS.gene51711:CDS |
| TTAAACTTACCATGAAATAT+AGG | 0.402640 | 3.4:-6819092 | None:intergenic |
| GATGCTGAAGCTTTAGAAAC+TGG | 0.405641 | 3.4:+6821456 | MS.gene51711:CDS |
| TTATCCCAGAAGGAATCCTT+TGG | 0.411650 | 3.4:-6820815 | None:intergenic |
| ATGACATTAAGGGCCAGTTT+AGG | 0.411844 | 3.4:-6821533 | None:intergenic |
| ATTTCCAAAGGATTCCTTCT+GGG | 0.412149 | 3.4:+6820811 | MS.gene51711:CDS |
| TAAGATTTGCTCGCATCTCC+GGG | 0.414274 | 3.4:+6827194 | MS.gene51711:CDS |
| GTGTTGATTGGTACCTTCAT+TGG | 0.414762 | 3.4:-6819959 | None:intergenic |
| TTCAAAACAATTGGTCCAGC+TGG | 0.422073 | 3.4:+6827885 | MS.gene51711:CDS |
| TGCAAAAGCTGATATTGAAA+TGG | 0.424887 | 3.4:-6820971 | None:intergenic |
| GGATGTTTAAATGGTTTGCT+TGG | 0.432632 | 3.4:+6819935 | MS.gene51711:CDS |
| GTTCAGTGCAGCTTGGGGCA+TGG | 0.433976 | 3.4:+6820443 | MS.gene51711:intron |
| TTTGGGAGGTTATTTGGCTC+AGG | 0.435359 | 3.4:+6820485 | MS.gene51711:CDS |
| TCCAGAAAGCATTGCTATGA+AGG | 0.448179 | 3.4:-6827339 | None:intergenic |
| GATCTTTGACATCAATATCA+TGG | 0.448413 | 3.4:+6819451 | MS.gene51711:CDS |
| TAAAGCTTCAGCATCATCAA+TGG | 0.462778 | 3.4:-6821448 | None:intergenic |
| ATGTCAATATTCAAAACAAT+TGG | 0.466702 | 3.4:+6827876 | MS.gene51711:CDS |
| GCCAAATAACCTCCCAAAGC+TGG | 0.467939 | 3.4:-6820480 | None:intergenic |
| TATGGAAAGAGAGATGATAT+AGG | 0.473840 | 3.4:-6819069 | None:intergenic |
| CACACTGCACCACCTCCAGC+TGG | 0.476747 | 3.4:-6827900 | None:intergenic |
| ATCTTTGACATCAATATCAT+GGG | 0.481621 | 3.4:+6819452 | MS.gene51711:CDS |
| CGTCAAAGAGGAGCAGCTAA+TGG | 0.482536 | 3.4:+6827837 | MS.gene51711:CDS |
| GATTTCAGTTCAGTGCAGCT+TGG | 0.482930 | 3.4:+6820436 | MS.gene51711:intron |
| AAGGGGTAACTTTGCAAAAG+AGG | 0.484476 | 3.4:-6827320 | None:intergenic |
| TGGTTTGCTTGGACCAATGA+AGG | 0.487662 | 3.4:+6819946 | MS.gene51711:CDS |
| CTTTAGAAACTGGAAGAAAT+GGG | 0.487764 | 3.4:+6821466 | MS.gene51711:CDS |
| TATGGGCGGTGAGTCCTAGA+AGG | 0.488224 | 3.4:+6826760 | MS.gene51711:CDS |
| GCGGTGAGTCCTAGAAGGTT+AGG | 0.507497 | 3.4:+6826765 | MS.gene51711:CDS |
| ACTGGAAGAAATGGGAAAGC+AGG | 0.507810 | 3.4:+6821474 | MS.gene51711:CDS |
| ACATTACACAACCACCCTCT+TGG | 0.508399 | 3.4:+6821417 | MS.gene51711:CDS |
| AAGTGCTTATGCTGGTTATG+TGG | 0.512014 | 3.4:+6819311 | MS.gene51711:CDS |
| GCTAATGGCATTGCTATGAC+TGG | 0.516266 | 3.4:+6827852 | MS.gene51711:CDS |
| CACTTAGAAGATTCTTCAGA+AGG | 0.517500 | 3.4:-6827394 | None:intergenic |
| GTAACTTTGCAAAAGAGGTA+TGG | 0.520424 | 3.4:-6827315 | None:intergenic |
| ACAACAATAGATGACATTAA+GGG | 0.521167 | 3.4:-6821543 | None:intergenic |
| ATGATGGGTATAGGGAAAGC+TGG | 0.534277 | 3.4:-6827150 | None:intergenic |
| TTTCAGGTGACCATAATCAC+TGG | 0.539650 | 3.4:+6827681 | MS.gene51711:intron |
| AAGTACTATGACAATTGTCC+TGG | 0.541237 | 3.4:+6818731 | MS.gene51711:CDS |
| CAATGGACTCATTGCCAAGA+GGG | 0.541868 | 3.4:-6821431 | None:intergenic |
| CTTACCATGAAATATAGGTA+TGG | 0.543067 | 3.4:-6819087 | None:intergenic |
| TTATGATATTTCTTATCAAG+AGG | 0.544878 | 3.4:+6821584 | MS.gene51711:CDS |
| TTAAGGGCCAGTTTAGGAAG+AGG | 0.546629 | 3.4:-6821527 | None:intergenic |
| ACATACCGGAAGCCAGATGC+AGG | 0.551614 | 3.4:-6821010 | None:intergenic |
| GTAGGATCTGCATTCATGCT+TGG | 0.560726 | 3.4:+6819426 | MS.gene51711:intron |
| AACTTCACAACTGAAAATGT+TGG | 0.560964 | 3.4:+6826795 | MS.gene51711:CDS |
| ATAATCCCTAAAATTGCCAC+AGG | 0.565902 | 3.4:-6819504 | None:intergenic |
| GGGAAGCAATATTAATCACT+AGG | 0.567739 | 3.4:-6827373 | None:intergenic |
| ATACCCAAATCTATTTCCAA+AGG | 0.569665 | 3.4:+6820799 | MS.gene51711:CDS |
| ACAATTGGTCCAGCTGGAGG+TGG | 0.572042 | 3.4:+6827891 | MS.gene51711:CDS |
| AAAGCTGATATTGAAATGGA+AGG | 0.572666 | 3.4:-6820967 | None:intergenic |
| CCAGAAAGCATTGCTATGAA+GGG | 0.575436 | 3.4:-6827338 | None:intergenic |
| GGAATCATAGCAGATCGTTA+CGG | 0.581193 | 3.4:+6819474 | MS.gene51711:CDS |
| AGCAAATCTTACAGGTCCAA+AGG | 0.586458 | 3.4:-6827182 | None:intergenic |
| ATTCGCTTACTTATCCCAGA+AGG | 0.592463 | 3.4:-6820825 | None:intergenic |
| AAATCTCTTGAACATATGGA+TGG | 0.593890 | 3.4:+6818802 | MS.gene51711:CDS |
| GAGGACATAAGTGCTTATGC+TGG | 0.604864 | 3.4:+6819303 | MS.gene51711:CDS |
| CAGAAAGCATTGCTATGAAG+GGG | 0.615996 | 3.4:-6827337 | None:intergenic |
| TTTCAGTTCAGTGCAGCTTG+GGG | 0.620914 | 3.4:+6820438 | MS.gene51711:intron |
| TCTCTTGAACATATGGATGG+TGG | 0.621234 | 3.4:+6818805 | MS.gene51711:CDS |
| GCTTTGTCAACTTTACAACC+AGG | 0.622810 | 3.4:-6818749 | None:intergenic |
| ACTTAGAAGATTCTTCAGAA+GGG | 0.622991 | 3.4:-6827393 | None:intergenic |
| TCTTATGCAAAATAGAGCTG+TGG | 0.626847 | 3.4:+6827710 | MS.gene51711:CDS |
| AAAACAATTGGTCCAGCTGG+AGG | 0.629594 | 3.4:+6827888 | MS.gene51711:CDS |
| AGTGCTTATGCTGGTTATGT+GGG | 0.629937 | 3.4:+6819312 | MS.gene51711:CDS |
| GTGAGTCCTAGAAGGTTAGG+TGG | 0.634850 | 3.4:+6826768 | MS.gene51711:CDS |
| GAACAGGAACAACGTCAAAG+AGG | 0.640183 | 3.4:+6827825 | MS.gene51711:intron |
| ATAGATCTTCTCATTATGGG+CGG | 0.644908 | 3.4:+6826746 | MS.gene51711:intron |
| ATAATTGGACCAGCTTTGGG+AGG | 0.649173 | 3.4:+6820471 | MS.gene51711:CDS |
| TGGACTCATTGCCAAGAGGG+TGG | 0.655428 | 3.4:-6821428 | None:intergenic |
| TTTGGATGGCCGTTACAACG+CGG | 0.675301 | 3.4:+6819903 | MS.gene51711:CDS |
| TCGTTACGGTAGAAAACCTG+TGG | 0.693560 | 3.4:+6819488 | MS.gene51711:CDS |
| TCTTTGACATCAATATCATG+GGG | 0.702667 | 3.4:+6819453 | MS.gene51711:CDS |
| TCAATGGACTCATTGCCAAG+AGG | 0.702775 | 3.4:-6821432 | None:intergenic |
| AAGATTTGCTCGCATCTCCG+GGG | 0.729111 | 3.4:+6827195 | MS.gene51711:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 6818692 | 6827923 | 6818692 | ID=MS.gene51711 |
| chr3.4 | mRNA | 6818692 | 6827923 | 6818692 | ID=MS.gene51711.t1;Parent=MS.gene51711 |
| chr3.4 | exon | 6818692 | 6818839 | 6818692 | ID=MS.gene51711.t1.exon1;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6818692 | 6818839 | 6818692 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| chr3.4 | exon | 6819064 | 6819104 | 6819064 | ID=MS.gene51711.t1.exon2;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6819064 | 6819104 | 6819064 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| chr3.4 | exon | 6819273 | 6819333 | 6819273 | ID=MS.gene51711.t1.exon3;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6819273 | 6819333 | 6819273 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| chr3.4 | exon | 6819430 | 6819532 | 6819430 | ID=MS.gene51711.t1.exon4;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6819430 | 6819532 | 6819430 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| chr3.4 | exon | 6819865 | 6819967 | 6819865 | ID=MS.gene51711.t1.exon5;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6819865 | 6819967 | 6819865 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| chr3.4 | exon | 6820089 | 6820142 | 6820089 | ID=MS.gene51711.t1.exon6;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6820089 | 6820142 | 6820089 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| chr3.4 | exon | 6820444 | 6820506 | 6820444 | ID=MS.gene51711.t1.exon7;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6820444 | 6820506 | 6820444 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| chr3.4 | exon | 6820788 | 6820837 | 6820788 | ID=MS.gene51711.t1.exon8;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6820788 | 6820837 | 6820788 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| chr3.4 | exon | 6820951 | 6821026 | 6820951 | ID=MS.gene51711.t1.exon9;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6820951 | 6821026 | 6820951 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| chr3.4 | exon | 6821414 | 6821605 | 6821414 | ID=MS.gene51711.t1.exon10;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6821414 | 6821605 | 6821414 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| chr3.4 | exon | 6826750 | 6826837 | 6826750 | ID=MS.gene51711.t1.exon11;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6826750 | 6826837 | 6826750 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| chr3.4 | exon | 6827131 | 6827216 | 6827131 | ID=MS.gene51711.t1.exon12;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6827131 | 6827216 | 6827131 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| chr3.4 | exon | 6827308 | 6827415 | 6827308 | ID=MS.gene51711.t1.exon13;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6827308 | 6827415 | 6827308 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| chr3.4 | exon | 6827687 | 6827731 | 6827687 | ID=MS.gene51711.t1.exon14;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6827687 | 6827731 | 6827687 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| chr3.4 | exon | 6827831 | 6827923 | 6827831 | ID=MS.gene51711.t1.exon15;Parent=MS.gene51711.t1 |
| chr3.4 | CDS | 6827831 | 6827923 | 6827831 | ID=cds.MS.gene51711.t1;Parent=MS.gene51711.t1 |
| Gene Sequence |
| Protein sequence |
