Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52116.t1 | XP_003607201.2 | 88.3 | 907 | 100 | 1 | 75 | 981 | 59 | 959 | 0.00E+00 | 1309.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52116.t1 | Q9LT25 | 54.2 | 840 | 310 | 18 | 127 | 958 | 60 | 832 | 1.1e-189 | 665.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52116.t1 | G7JNF7 | 88.3 | 907 | 100 | 1 | 75 | 981 | 59 | 959 | 0.0e+00 | 1309.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene055184 | MS.gene52116 | PPI |
| MS.gene67685 | MS.gene52116 | PPI |
| MS.gene62648 | MS.gene52116 | PPI |
| MS.gene89818 | MS.gene52116 | PPI |
| MS.gene89294 | MS.gene52116 | PPI |
| MS.gene040175 | MS.gene52116 | PPI |
| MS.gene52116 | MS.gene005567 | PPI |
| MS.gene07236 | MS.gene52116 | PPI |
| MS.gene025726 | MS.gene52116 | PPI |
| MS.gene52116 | MS.gene22969 | PPI |
| MS.gene055186 | MS.gene52116 | PPI |
| MS.gene46089 | MS.gene52116 | PPI |
| MS.gene52116 | MS.gene06988 | PPI |
| MS.gene063673 | MS.gene52116 | PPI |
| MS.gene95860 | MS.gene52116 | PPI |
| MS.gene52116 | MS.gene20758 | PPI |
| MS.gene67503 | MS.gene52116 | PPI |
| MS.gene52116 | MS.gene89296 | PPI |
| MS.gene025725 | MS.gene52116 | PPI |
| MS.gene64820 | MS.gene52116 | PPI |
| MS.gene89820 | MS.gene52116 | PPI |
| MS.gene015612 | MS.gene52116 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52116.t1 | MTR_4g074440 | 94.444 | 936 | 40 | 4 | 47 | 981 | 35 | 959 | 0.0 | 1691 |
| MS.gene52116.t1 | MTR_4g074440 | 92.190 | 653 | 39 | 4 | 47 | 698 | 35 | 676 | 0.0 | 1149 |
| MS.gene52116.t1 | MTR_1g017520 | 24.742 | 776 | 438 | 24 | 202 | 915 | 111 | 802 | 2.24e-39 | 159 |
| MS.gene52116.t1 | MTR_1g106025 | 24.952 | 521 | 315 | 14 | 293 | 768 | 164 | 653 | 1.12e-29 | 127 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52116.t1 | AT3G19840 | 54.632 | 842 | 307 | 18 | 127 | 960 | 60 | 834 | 0.0 | 756 |
| MS.gene52116.t1 | AT1G44910 | 28.802 | 434 | 250 | 14 | 359 | 765 | 190 | 591 | 7.32e-37 | 150 |
| MS.gene52116.t1 | AT1G44910 | 28.802 | 434 | 250 | 14 | 359 | 765 | 190 | 591 | 8.51e-37 | 150 |
| MS.gene52116.t1 | AT3G19670 | 25.875 | 657 | 381 | 26 | 186 | 768 | 16 | 640 | 2.53e-26 | 117 |
| MS.gene52116.t1 | AT3G19670 | 25.875 | 657 | 381 | 26 | 186 | 768 | 16 | 640 | 2.53e-26 | 117 |
| MS.gene52116.t1 | AT3G19670 | 25.875 | 657 | 381 | 26 | 186 | 768 | 16 | 640 | 2.53e-26 | 117 |
| MS.gene52116.t1 | AT3G19670 | 27.152 | 453 | 272 | 14 | 356 | 768 | 187 | 621 | 4.08e-26 | 116 |
Find 276 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAAACCTGCTGGCTTTAA+AGG | 0.103135 | 4.1:-29543955 | MS.gene52116:CDS |
| ACTATCGCCCAACTACTTTA+TGG | 0.163328 | 4.1:-29544706 | MS.gene52116:CDS |
| TCTTCAGTGGCTTTCTTTAA+AGG | 0.171523 | 4.1:+29535966 | None:intergenic |
| TCAGACAATACAGTAGTATT+TGG | 0.190956 | 4.1:+29540569 | None:intergenic |
| GGAGGGTTGACAGCCGAATT+AGG | 0.218520 | 4.1:+29546410 | None:intergenic |
| CAACAAAATGTTAACGCTTC+TGG | 0.226037 | 4.1:-29546574 | MS.gene52116:CDS |
| GAGAGTGGGTGTTTGGAGTT+AGG | 0.251045 | 4.1:+29546999 | None:intergenic |
| GGGAGCGAGAGCTGCGTAAA+AGG | 0.255583 | 4.1:-29533700 | MS.gene52116:CDS |
| CTTGGAACCTTGTTATATCT+TGG | 0.278928 | 4.1:+29532501 | None:intergenic |
| ACAACCCATCACACTCAATT+AGG | 0.303806 | 4.1:-29546930 | MS.gene52116:intron |
| AAAGTAGTTGGGCGATAGTT+AGG | 0.309852 | 4.1:+29544710 | None:intergenic |
| AACGAGAGACCAGAGCTAAA+AGG | 0.310860 | 4.1:-29534160 | MS.gene52116:CDS |
| TTGCTCCACCTCAGGGATAT+TGG | 0.327307 | 4.1:-29544457 | MS.gene52116:CDS |
| CGAGGATCAAATACTATCTT+TGG | 0.331317 | 4.1:+29538306 | None:intergenic |
| AAAAGCTGGCGGCGCACTTT+GGG | 0.332039 | 4.1:+29546316 | None:intergenic |
| CTAGGAGTTCCGGGCATTAC+AGG | 0.339967 | 4.1:+29544653 | None:intergenic |
| AACAAAATGTTAACGCTTCT+GGG | 0.340217 | 4.1:-29546573 | MS.gene52116:CDS |
| ACCGGGAGTACTAGGAGTTC+CGG | 0.345684 | 4.1:+29544643 | None:intergenic |
| CCCTGATTCCAATGGATGTT+TGG | 0.347086 | 4.1:+29532356 | None:intergenic |
| CTAGGAACTTACATTGAAAC+TGG | 0.351863 | 4.1:+29543515 | None:intergenic |
| ATAAACCTGCTGGCTTTAAA+GGG | 0.355108 | 4.1:-29543954 | MS.gene52116:CDS |
| AAATCTTGTGTGGCGGAATC+AGG | 0.355244 | 4.1:+29544815 | None:intergenic |
| TTCAATTCCGCCACCTCATT+GGG | 0.357442 | 4.1:+29540638 | None:intergenic |
| TCCAAACATCCATTGGAATC+AGG | 0.357482 | 4.1:-29532357 | MS.gene52116:CDS |
| TAAAAGCTGGCGGCGCACTT+TGG | 0.358326 | 4.1:+29546315 | None:intergenic |
| TTTCAATTCCGCCACCTCAT+TGG | 0.363697 | 4.1:+29540637 | None:intergenic |
| AAGAAGAAGTTGCAGGAATC+AGG | 0.365897 | 4.1:-29540431 | MS.gene52116:CDS |
| GGAAAGAGAAGAACATGAAA+TGG | 0.366620 | 4.1:-29533675 | MS.gene52116:CDS |
| TGATTCACGGTTTGAGGCAT+TGG | 0.369933 | 4.1:-29537791 | MS.gene52116:CDS |
| TTTATGCACTCCTCCTTTGA+AGG | 0.371786 | 4.1:+29540218 | None:intergenic |
| GGAGTGCATAAATCAATTTA+AGG | 0.377125 | 4.1:-29540207 | MS.gene52116:intron |
| GAGGGTTGACAGCCGAATTA+GGG | 0.385234 | 4.1:+29546411 | None:intergenic |
| AACTCCGGAGAGTGGGTGTT+TGG | 0.392322 | 4.1:+29546992 | None:intergenic |
| TGTTTCACAGATTTATATCT+CGG | 0.392372 | 4.1:+29534248 | None:intergenic |
| ATCCATGGTTGCTCCACCTC+AGG | 0.393076 | 4.1:-29544465 | MS.gene52116:CDS |
| TACTATTATAATGCTTTGAC+AGG | 0.395407 | 4.1:-29543991 | MS.gene52116:CDS |
| CAGGATGGACGGGAAAAGTT+GGG | 0.396058 | 4.1:+29544672 | None:intergenic |
| GGCATTGGACCGCAAGGAAC+GGG | 0.399005 | 4.1:-29537776 | MS.gene52116:CDS |
| AATGGAGGTTGAGCAGGAAT+AGG | 0.399167 | 4.1:+29546389 | None:intergenic |
| GAACCATTTGATTCGGATCC+TGG | 0.400634 | 4.1:+29540365 | None:intergenic |
| AATGAAGGGGCAGCTGGACG+TGG | 0.406522 | 4.1:+29546353 | None:intergenic |
| AGCTGAATCAAGATCAGAAT+TGG | 0.409415 | 4.1:+29532918 | None:intergenic |
| GCAGCAGGATATTGATGAAA+TGG | 0.411046 | 4.1:+29544398 | None:intergenic |
| GACTTCTGAAGCCGCGTCTC+AGG | 0.411196 | 4.1:-29532589 | MS.gene52116:CDS |
| CTTTCAACTCTCGTTGGTCC+AGG | 0.414381 | 4.1:-29535872 | MS.gene52116:CDS |
| ACACCAGGAGGTTGAGAATC+AGG | 0.415316 | 4.1:+29544317 | None:intergenic |
| TGGAGTTAGGGCTTATTGCC+TGG | 0.421555 | 4.1:+29547012 | None:intergenic |
| CATCTGCTGAGGGGAACATC+TGG | 0.424887 | 4.1:-29544248 | MS.gene52116:CDS |
| GAAGGGGCAGCTGGACGTGG+GGG | 0.427000 | 4.1:+29546356 | None:intergenic |
| GCATATATTCTTAGGCATCA+TGG | 0.428010 | 4.1:-29532987 | MS.gene52116:intron |
| CAGACAATACAGTAGTATTT+GGG | 0.428630 | 4.1:+29540570 | None:intergenic |
| TGGTGAGCTTTGTACGATAG+AGG | 0.431267 | 4.1:+29540472 | None:intergenic |
| GGAATAGAAGAGGATGTAAC+AGG | 0.431810 | 4.1:+29540404 | None:intergenic |
| GGGAAATGATTCACGGTTTG+AGG | 0.433155 | 4.1:-29537797 | MS.gene52116:CDS |
| AGAGTGGGTGTTTGGAGTTA+GGG | 0.434063 | 4.1:+29547000 | None:intergenic |
| CGCAGGACAAGCTGCGGGAG+AGG | 0.437446 | 4.1:-29533721 | MS.gene52116:intron |
| TGAAGGGGCAGCTGGACGTG+GGG | 0.437614 | 4.1:+29546355 | None:intergenic |
| GCAGGGGATGAACCCTAATT+CGG | 0.438054 | 4.1:-29546423 | MS.gene52116:intron |
| GGAAAACCTGTGCTTGTAGA+TGG | 0.438272 | 4.1:+29544572 | None:intergenic |
| TAATTGAGTGTGATGGGTTG+TGG | 0.439676 | 4.1:+29546932 | None:intergenic |
| TTTGATCAAGAAGAAGTTGC+AGG | 0.440037 | 4.1:-29540438 | MS.gene52116:CDS |
| CAGAGCTAAAAGGGAGGAGC+AGG | 0.441328 | 4.1:-29534150 | MS.gene52116:intron |
| TGTTGGGCGGCGTAAGAGAT+TGG | 0.442498 | 4.1:+29546592 | None:intergenic |
| TATTGTAGGGTAGATTTGCC+TGG | 0.442537 | 4.1:-29542454 | MS.gene52116:intron |
| CATGGATGGTATGGGTGGAT+AGG | 0.443598 | 4.1:+29544481 | None:intergenic |
| GGATCATGTAAAGATGCTTC+AGG | 0.444198 | 4.1:-29532875 | MS.gene52116:CDS |
| GGAAAACAGTGCTTAATTCA+TGG | 0.444271 | 4.1:-29532554 | MS.gene52116:CDS |
| ACCACGTGCTGGAAATGAAA+AGG | 0.444633 | 4.1:+29544364 | None:intergenic |
| GTATTGTCTGAGAGAGGGTC+TGG | 0.444793 | 4.1:-29540557 | MS.gene52116:CDS |
| CTTATTGCCTGGCTGACCAT+TGG | 0.446750 | 4.1:+29547023 | None:intergenic |
| TAGATTTGCCTGGTACTGAT+TGG | 0.446942 | 4.1:-29542444 | MS.gene52116:CDS |
| TGCACAAAAGGCCGCAATTG+AGG | 0.448302 | 4.1:-29538009 | MS.gene52116:CDS |
| ACAGGATGGACGGGAAAAGT+TGG | 0.450966 | 4.1:+29544671 | None:intergenic |
| ACGAGAGACCAGAGCTAAAA+GGG | 0.452784 | 4.1:-29534159 | MS.gene52116:CDS |
| GCACAAAAGGCCGCAATTGA+GGG | 0.453511 | 4.1:-29538008 | MS.gene52116:CDS |
| TGGAGCAACCATGGATGGTA+TGG | 0.453607 | 4.1:+29544472 | None:intergenic |
| GGAAAATTATATGAAAATGA+AGG | 0.455482 | 4.1:+29546338 | None:intergenic |
| AGCAGCGGGAGCAGAAATGC+TGG | 0.457214 | 4.1:+29544277 | None:intergenic |
| GATGGGTTGTGGGGTGAGAG+AGG | 0.461936 | 4.1:+29546943 | None:intergenic |
| CCACGTGCTGGAAATGAAAA+GGG | 0.462872 | 4.1:+29544365 | None:intergenic |
| GGAGGTTGAGCAGGAATAGG+AGG | 0.463244 | 4.1:+29546392 | None:intergenic |
| CTCTAGAGACTTTCTACCTC+TGG | 0.463701 | 4.1:+29532383 | None:intergenic |
| GATTCAGAAGACGAGGATAG+TGG | 0.464902 | 4.1:-29540242 | MS.gene52116:CDS |
| TGTTCCCCTCAGCAGATGAC+TGG | 0.465491 | 4.1:+29544253 | None:intergenic |
| AAGCCTCTATGTGAGGCATT+AGG | 0.468749 | 4.1:+29544506 | None:intergenic |
| CCCGTCCATCCTGTAATGCC+CGG | 0.470449 | 4.1:-29544662 | MS.gene52116:CDS |
| TCGGATCCTGGTTGCACTGA+TGG | 0.472882 | 4.1:+29540377 | None:intergenic |
| AATTGAGTGTGATGGGTTGT+GGG | 0.474417 | 4.1:+29546933 | None:intergenic |
| TATGAAAATGAAGGGGCAGC+TGG | 0.474627 | 4.1:+29546347 | None:intergenic |
| ACCTCCCCTTTAAAGCCAGC+AGG | 0.476497 | 4.1:+29543949 | None:intergenic |
| ATCTGTGAAACATGAGGATA+GGG | 0.476617 | 4.1:-29534237 | MS.gene52116:CDS |
| CCGGGAGTACTAGGAGTTCC+GGG | 0.479194 | 4.1:+29544644 | None:intergenic |
| ACGGGAAAAGTTGGGGCAGT+TGG | 0.480355 | 4.1:+29544680 | None:intergenic |
| GAAATGCTGGCAGCACCAAC+AGG | 0.482497 | 4.1:+29544290 | None:intergenic |
| AAGGGGCAGCTGGACGTGGG+GGG | 0.482647 | 4.1:+29546357 | None:intergenic |
| GCCTGGTACTGATTGGCAGT+TGG | 0.484186 | 4.1:-29542437 | MS.gene52116:CDS |
| CTTAACCTAATTGAGTGTGA+TGG | 0.485251 | 4.1:+29546925 | None:intergenic |
| GTTTCACAGATTTATATCTC+GGG | 0.486215 | 4.1:+29534249 | None:intergenic |
| CAATCCCTGCTTCAGTCTTG+TGG | 0.487147 | 4.1:+29544016 | None:intergenic |
| GCTGGAAATGAAAAGGGGCC+AGG | 0.489782 | 4.1:+29544371 | None:intergenic |
| CAGGCTCTTGGCTGTAGAGT+CGG | 0.490013 | 4.1:+29540334 | None:intergenic |
| ATAGAAGTAGGAGCAGGAAC+AGG | 0.491114 | 4.1:+29544758 | None:intergenic |
| AATCCCTGCTTCAGTCTTGT+GGG | 0.491851 | 4.1:+29544017 | None:intergenic |
| TGCAGCTCCGACTTCATCAC+CGG | 0.494573 | 4.1:-29546620 | MS.gene52116:intron |
| ATGGATGGTATGGGTGGATA+GGG | 0.495281 | 4.1:+29544482 | None:intergenic |
| AGCATCTAGCTGATCATTAG+CGG | 0.495789 | 4.1:+29544047 | None:intergenic |
| TGCGATCTCCCTGATTCCAA+TGG | 0.496991 | 4.1:+29532348 | None:intergenic |
| GAAGTAATTTCAGCACATAC+TGG | 0.497178 | 4.1:-29544215 | MS.gene52116:intron |
| AACTCCAAACACCCACTCTC+CGG | 0.497734 | 4.1:-29546996 | MS.gene52116:CDS |
| GAAAATTATATGAAAATGAA+GGG | 0.497891 | 4.1:+29546339 | None:intergenic |
| AATCTGTGAAACATGAGGAT+AGG | 0.498829 | 4.1:-29534238 | MS.gene52116:CDS |
| GTATGAAATGGAGGTTGAGC+AGG | 0.499181 | 4.1:+29546383 | None:intergenic |
| GTTGGCATCCATAAAGTAGT+TGG | 0.499359 | 4.1:+29544698 | None:intergenic |
| AGAGTCTTCCAAACATCCAT+TGG | 0.500125 | 4.1:-29532364 | MS.gene52116:CDS |
| TGCTTCCAGTCATCTGCTGA+GGG | 0.501155 | 4.1:-29544258 | MS.gene52116:CDS |
| GATCTCCTAGCAGAATAGCT+AGG | 0.504237 | 4.1:+29538089 | None:intergenic |
| ATGAAGGGGCAGCTGGACGT+GGG | 0.505446 | 4.1:+29546354 | None:intergenic |
| ACAAAGCTCACCATCGGCAC+TGG | 0.505649 | 4.1:-29540462 | MS.gene52116:CDS |
| CCTCCTGGTGTTACTCCTGT+TGG | 0.507077 | 4.1:-29544305 | MS.gene52116:CDS |
| CCAAACATCCATTGGAATCA+GGG | 0.507489 | 4.1:-29532356 | MS.gene52116:CDS |
| GAATCAGGTACTGGAACAGC+AGG | 0.507999 | 4.1:+29544332 | None:intergenic |
| ATAGAGAAGCCTTGTGGCGC+CGG | 0.508501 | 4.1:-29532473 | MS.gene52116:CDS |
| TGTAGATGGAGCAGCTGGAT+TGG | 0.509082 | 4.1:+29544586 | None:intergenic |
| GGGAGTGCACCACCACGTGC+TGG | 0.511437 | 4.1:+29544353 | None:intergenic |
| ACTGCCCACAAGACTGAAGC+AGG | 0.511530 | 4.1:-29544021 | MS.gene52116:CDS |
| GAGAAGAACATGAAATGGAA+AGG | 0.513731 | 4.1:-29533670 | MS.gene52116:CDS |
| CTAATGATCAGCTAGATGCT+TGG | 0.516038 | 4.1:-29544044 | MS.gene52116:CDS |
| TGATCTTGATTCAGCTGACA+TGG | 0.519037 | 4.1:-29532911 | MS.gene52116:CDS |
| CGAGGATAGTGGGCCTTCAA+AGG | 0.520405 | 4.1:-29540231 | MS.gene52116:CDS |
| CAGTTGGTCTCTACTAGTGA+TGG | 0.521306 | 4.1:-29542421 | MS.gene52116:CDS |
| AAGTAGTTGGGCGATAGTTA+GGG | 0.521359 | 4.1:+29544711 | None:intergenic |
| CAACCAGGATCCGAATCAAA+TGG | 0.524617 | 4.1:-29540368 | MS.gene52116:CDS |
| GAATCATATCCTCTGCATAC+CGG | 0.525933 | 4.1:+29532454 | None:intergenic |
| TTGGCATCCATAAAGTAGTT+GGG | 0.527790 | 4.1:+29544699 | None:intergenic |
| GTACCTGATTCTCAACCTCC+TGG | 0.528552 | 4.1:-29544320 | MS.gene52116:CDS |
| GTGCTTGTAGATGGAGCAGC+TGG | 0.528608 | 4.1:+29544581 | None:intergenic |
| CTGCTTCCAGTCATCTGCTG+AGG | 0.530922 | 4.1:-29544259 | MS.gene52116:CDS |
| GATGGAGCAGCTGGATTGGA+GGG | 0.531593 | 4.1:+29544590 | None:intergenic |
| GGATTGGAGGGTAATATCAC+AGG | 0.531763 | 4.1:+29544602 | None:intergenic |
| AAAATCATCTCATGATTCAA+AGG | 0.532449 | 4.1:-29532424 | MS.gene52116:CDS |
| TGAAGTGCCAATGGTCAGCC+AGG | 0.538775 | 4.1:-29547030 | None:intergenic |
| AGCAGATGACTGGAAGCAGC+GGG | 0.541855 | 4.1:+29544263 | None:intergenic |
| GGAGAGAATCAAAGACCCTA+TGG | 0.543294 | 4.1:-29533591 | MS.gene52116:intron |
| AATGGTTACTCTGAATGCTC+CGG | 0.543813 | 4.1:-29540534 | MS.gene52116:CDS |
| GCAGGACAAGCTGCGGGAGA+GGG | 0.545405 | 4.1:-29533720 | MS.gene52116:intron |
| CTCTGCATACCGGCGCCACA+AGG | 0.546986 | 4.1:+29532464 | None:intergenic |
| GAGGTTGAGCAGGAATAGGA+GGG | 0.547528 | 4.1:+29546393 | None:intergenic |
| TCTTTCTCCCACTTTGAGAA+CGG | 0.547622 | 4.1:+29538333 | None:intergenic |
| TCAACATATGATAAACCTGC+TGG | 0.549166 | 4.1:-29543964 | MS.gene52116:CDS |
| AATGCTCCGGCTATTACCAC+TGG | 0.549560 | 4.1:-29540521 | MS.gene52116:CDS |
| GAATTGGTTGCACGCCCTTG+AGG | 0.549986 | 4.1:+29532934 | None:intergenic |
| CAGTGTTAGATTGCCTGTGT+TGG | 0.550015 | 4.1:+29546285 | None:intergenic |
| TTGTCTCGGTACTGCAGCTG+AGG | 0.550217 | 4.1:+29544550 | None:intergenic |
| AGAAGAACATGAAATGGAAA+GGG | 0.550807 | 4.1:-29533669 | MS.gene52116:CDS |
| GCATTAGGATCTGATGCAGT+TGG | 0.551297 | 4.1:+29544521 | None:intergenic |
| GGTGAGCTTTGTACGATAGA+GGG | 0.552025 | 4.1:+29540473 | None:intergenic |
| TCCGGGCATTACAGGATGGA+CGG | 0.554977 | 4.1:+29544661 | None:intergenic |
| AGAAATGGGGAAATGATTCA+CGG | 0.555164 | 4.1:-29537804 | MS.gene52116:CDS |
| CCGGGCATTACAGGATGGAC+GGG | 0.555217 | 4.1:+29544662 | None:intergenic |
| AAATAACTTTCAACTCTCGT+TGG | 0.555691 | 4.1:-29535878 | MS.gene52116:CDS |
| TGGGCGGCGTAAGAGATTGG+TGG | 0.556189 | 4.1:+29546595 | None:intergenic |
| ATCTCCTAGCAGAATAGCTA+GGG | 0.556877 | 4.1:+29538090 | None:intergenic |
| TTTCAACTCTCGTTGGTCCA+GGG | 0.557412 | 4.1:-29535871 | MS.gene52116:intron |
| GGAGGTTGAGAATCAGGTAC+TGG | 0.557933 | 4.1:+29544323 | None:intergenic |
| GATCCTAATGCCTCACATAG+AGG | 0.558216 | 4.1:-29544509 | MS.gene52116:CDS |
| AGGCATTGGACCGCAAGGAA+CGG | 0.559314 | 4.1:-29537777 | MS.gene52116:CDS |
| CCCGGAACTCCTAGTACTCC+CGG | 0.561127 | 4.1:-29544644 | MS.gene52116:CDS |
| AAGGGGCCAGGAAAAGCAGC+AGG | 0.561207 | 4.1:+29544383 | None:intergenic |
| AAGTCAATGCTTAAAGAACA+AGG | 0.563683 | 4.1:-29535903 | MS.gene52116:CDS |
| TATTGTATCAGACAAGCTGC+TGG | 0.567030 | 4.1:-29540667 | MS.gene52116:intron |
| TGGTTCGCAGGACAAGCTGC+GGG | 0.567632 | 4.1:-29533726 | MS.gene52116:intron |
| AGATGGAGCAGCTGGATTGG+AGG | 0.568440 | 4.1:+29544589 | None:intergenic |
| AAAGCTGGCGGCGCACTTTG+GGG | 0.571914 | 4.1:+29546317 | None:intergenic |
| TGTTGGTTGGCAGTAAAAGC+TGG | 0.571931 | 4.1:+29546302 | None:intergenic |
| TGATGGAGTAGGAATAGAAG+AGG | 0.573030 | 4.1:+29540394 | None:intergenic |
| GTTAGATTGCCTGTGTTGGT+TGG | 0.576192 | 4.1:+29546289 | None:intergenic |
| GAGTGGGCTGCACAGCAACT+TGG | 0.577693 | 4.1:+29543537 | None:intergenic |
| GCAAATTCCCAATGAGGTGG+CGG | 0.578570 | 4.1:-29540645 | MS.gene52116:CDS |
| CTATCCCTAGCTATTCTGCT+AGG | 0.579222 | 4.1:-29538094 | MS.gene52116:CDS |
| CGACCACCAGTGGTAATAGC+CGG | 0.580608 | 4.1:+29540515 | None:intergenic |
| ACCAACTGCCAATCAGTACC+AGG | 0.581773 | 4.1:+29542436 | None:intergenic |
| GGCATTGCGGATATAGAAGT+AGG | 0.582367 | 4.1:+29544746 | None:intergenic |
| TTAACCTAATTGAGTGTGAT+GGG | 0.583862 | 4.1:+29546926 | None:intergenic |
| CGTGGCACCGTTCTCAAAGT+GGG | 0.584929 | 4.1:-29538340 | MS.gene52116:CDS |
| GGAGCAACCATGGATGGTAT+GGG | 0.585140 | 4.1:+29544473 | None:intergenic |
| GCGTGGCACCGTTCTCAAAG+TGG | 0.585273 | 4.1:-29538341 | MS.gene52116:CDS |
| GCTGCTCCATCTACAAGCAC+AGG | 0.585375 | 4.1:-29544578 | MS.gene52116:CDS |
| ATGTTCAACAGCTTTCAACT+CGG | 0.586994 | 4.1:+29534190 | None:intergenic |
| AAGGCTTCTCTATCTTCACT+TGG | 0.587101 | 4.1:+29532483 | None:intergenic |
| AGTTGGTCTCTACTAGTGAT+GGG | 0.588232 | 4.1:-29542420 | MS.gene52116:CDS |
| CCTGGTTGCACTGATGGAGT+AGG | 0.588394 | 4.1:+29540383 | None:intergenic |
| TCCCTGAGGTGGAGCAACCA+TGG | 0.588442 | 4.1:+29544463 | None:intergenic |
| CGCCACTGTGACACAGAATG+AGG | 0.588689 | 4.1:-29544073 | MS.gene52116:CDS |
| TCAGTCACAACTCCGGAGAG+TGG | 0.588725 | 4.1:+29546984 | None:intergenic |
| CCTACTCCATCAGTGCAACC+AGG | 0.588729 | 4.1:-29540383 | MS.gene52116:CDS |
| ACTACTGTATTGTCTGAGAG+AGG | 0.589570 | 4.1:-29540563 | MS.gene52116:CDS |
| CACAAAAGGCCGCAATTGAG+GGG | 0.591059 | 4.1:-29538007 | MS.gene52116:CDS |
| GCGAGAGCTGCGTAAAAGGA+AGG | 0.591852 | 4.1:-29533696 | MS.gene52116:CDS |
| GAAGCATCTTTACATGATCC+CGG | 0.591894 | 4.1:+29532878 | None:intergenic |
| TGAGGTGGAGCAACCATGGA+TGG | 0.592573 | 4.1:+29544467 | None:intergenic |
| CACTTTACTAAAATCTTGTG+TGG | 0.592921 | 4.1:+29544805 | None:intergenic |
| CTGGTTCGCAGGACAAGCTG+CGG | 0.592961 | 4.1:-29533727 | MS.gene52116:intron |
| CTATCCACCCATACCATCCA+TGG | 0.595305 | 4.1:-29544480 | MS.gene52116:CDS |
| AGGATGGACGGGAAAAGTTG+GGG | 0.596070 | 4.1:+29544673 | None:intergenic |
| TCCATGGTTGCTCCACCTCA+GGG | 0.597381 | 4.1:-29544464 | MS.gene52116:CDS |
| GCGTAAGAGATTGGTGGCGC+CGG | 0.597503 | 4.1:+29546601 | None:intergenic |
| TTGATCAAATCCAGTGCCGA+TGG | 0.597511 | 4.1:+29540452 | None:intergenic |
| TCGTGCGGAAGAAGAACGCA+AGG | 0.598559 | 4.1:-29538042 | MS.gene52116:CDS |
| GATGCTTAAGGAGCGTGGCG+TGG | 0.599149 | 4.1:-29538358 | MS.gene52116:CDS |
| ATTCAGAAGACGAGGATAGT+GGG | 0.600872 | 4.1:-29540241 | MS.gene52116:CDS |
| GGGAGCATCTTCTGAACGAA+AGG | 0.601329 | 4.1:-29537756 | MS.gene52116:intron |
| GCTCCGGCTATTACCACTGG+TGG | 0.602029 | 4.1:-29540518 | MS.gene52116:CDS |
| GGTATGCAGAGGATATGATT+CGG | 0.602226 | 4.1:-29532452 | MS.gene52116:CDS |
| GCGGATATAGAAGTAGGAGC+AGG | 0.603753 | 4.1:+29544752 | None:intergenic |
| TTTACTAAAATCTTGTGTGG+CGG | 0.603992 | 4.1:+29544808 | None:intergenic |
| CTGGCAAATTCCCAATGAGG+TGG | 0.604078 | 4.1:-29540648 | MS.gene52116:CDS |
| AACTTACATTGAAACTGGAG+TGG | 0.604902 | 4.1:+29543520 | None:intergenic |
| GTCTGAGAGAGGGTCTGGAA+TGG | 0.605847 | 4.1:-29540552 | MS.gene52116:CDS |
| GCTATTAGACGAAGCCTCAG+AGG | 0.606226 | 4.1:-29537976 | MS.gene52116:intron |
| GGTGGCGCCGGTGATGAAGT+CGG | 0.606866 | 4.1:+29546613 | None:intergenic |
| CACAGATGAGTGGTGTACAT+AGG | 0.606997 | 4.1:-29544424 | MS.gene52116:CDS |
| GAAGATGCTCCCGTTCCTTG+CGG | 0.608703 | 4.1:+29537767 | None:intergenic |
| TAGGAGATGCTTAAGGAGCG+TGG | 0.613437 | 4.1:-29538363 | MS.gene52116:CDS |
| CAGTCACAACTCCGGAGAGT+GGG | 0.613523 | 4.1:+29546985 | None:intergenic |
| TGTCTCGGTACTGCAGCTGA+GGG | 0.614677 | 4.1:+29544551 | None:intergenic |
| TTTAACATAATGTTCAAACA+AGG | 0.617075 | 4.1:+29538067 | None:intergenic |
| GTAGTATTTGGGACGGACGT+TGG | 0.617666 | 4.1:+29540581 | None:intergenic |
| ACCTGCTGGCTTTAAAGGGG+AGG | 0.618538 | 4.1:-29543950 | MS.gene52116:intron |
| CTGCTTAAACCCCTCAATTG+CGG | 0.621226 | 4.1:+29537998 | None:intergenic |
| GAGCAGATCAGTCACAACTC+CGG | 0.623675 | 4.1:+29546977 | None:intergenic |
| CAGCAGATGACTGGAAGCAG+CGG | 0.624812 | 4.1:+29544262 | None:intergenic |
| TACAGCATCACGACCACCAG+TGG | 0.625947 | 4.1:+29540505 | None:intergenic |
| GCAACCATGGATGGTATGGG+TGG | 0.628786 | 4.1:+29544476 | None:intergenic |
| CGTCTCAGGAAACTGACGAT+GGG | 0.632849 | 4.1:-29532575 | MS.gene52116:CDS |
| TATCGTACAAAGCTCACCAT+CGG | 0.634824 | 4.1:-29540468 | MS.gene52116:CDS |
| GTTTGAGGCATTGGACCGCA+AGG | 0.636101 | 4.1:-29537782 | MS.gene52116:CDS |
| ATCTGATCCAAGATATAACA+AGG | 0.636281 | 4.1:-29532508 | MS.gene52116:CDS |
| ACTTACATTGAAACTGGAGT+GGG | 0.636689 | 4.1:+29543521 | None:intergenic |
| GAGTTCCGGGCATTACAGGA+TGG | 0.639013 | 4.1:+29544657 | None:intergenic |
| CTACTGTATTGTCTGAGAGA+GGG | 0.639250 | 4.1:-29540562 | MS.gene52116:CDS |
| GCAGGATATTGATGAAATGG+TGG | 0.640371 | 4.1:+29544401 | None:intergenic |
| GCACCAACAGGAGTAACACC+AGG | 0.640657 | 4.1:+29544302 | None:intergenic |
| ATCGTCAGTTTCCTGAGACG+CGG | 0.642398 | 4.1:+29532578 | None:intergenic |
| CTATGTACACCACTCATCTG+TGG | 0.642650 | 4.1:+29544425 | None:intergenic |
| CTTGTGGCGCCGGTATGCAG+AGG | 0.645188 | 4.1:-29532463 | MS.gene52116:CDS |
| ATCTTCAGATTCAGAAGACG+AGG | 0.645316 | 4.1:-29540249 | MS.gene52116:CDS |
| CGATAGTTAGGGTCAGAGCA+AGG | 0.646253 | 4.1:+29544722 | None:intergenic |
| ATTGAGTGTGATGGGTTGTG+GGG | 0.648145 | 4.1:+29546934 | None:intergenic |
| CACGTGCTGGAAATGAAAAG+GGG | 0.651381 | 4.1:+29544366 | None:intergenic |
| GGATAGTGGGCCTTCAAAGG+AGG | 0.652790 | 4.1:-29540228 | MS.gene52116:CDS |
| ATATAAATCTGTGAAACATG+AGG | 0.654245 | 4.1:-29534243 | MS.gene52116:CDS |
| TAAACCTGCTGGCTTTAAAG+GGG | 0.658608 | 4.1:-29543953 | MS.gene52116:CDS |
| TGTAACCAATATCCCTGAGG+TGG | 0.659237 | 4.1:+29544452 | None:intergenic |
| GTCAGAGCAAGGCGGCATTG+CGG | 0.659633 | 4.1:+29544733 | None:intergenic |
| AAAATTATATGAAAATGAAG+GGG | 0.660721 | 4.1:+29546340 | None:intergenic |
| TGGTTGGCAGTAAAAGCTGG+CGG | 0.670102 | 4.1:+29546305 | None:intergenic |
| GCGTCTCAGGAAACTGACGA+TGG | 0.673809 | 4.1:-29532576 | MS.gene52116:CDS |
| TGAGGCAACATTAACCTCTG+AGG | 0.674577 | 4.1:+29537962 | None:intergenic |
| CAATACAGTAGTATTTGGGA+CGG | 0.675266 | 4.1:+29540574 | None:intergenic |
| TTACAGCCTCCACAGATGAG+TGG | 0.676991 | 4.1:-29544434 | MS.gene52116:CDS |
| TTACTATAACAATCGAACCA+AGG | 0.679735 | 4.1:-29542389 | MS.gene52116:intron |
| TGTACACCACTCATCTGTGG+AGG | 0.681421 | 4.1:+29544428 | None:intergenic |
| CTGCTGGCAAATTCCCAATG+AGG | 0.682038 | 4.1:-29540651 | MS.gene52116:CDS |
| GCTTCCAGTCATCTGCTGAG+GGG | 0.683518 | 4.1:-29544257 | MS.gene52116:CDS |
| AGAGACCAGAGCTAAAAGGG+AGG | 0.686025 | 4.1:-29534156 | MS.gene52116:CDS |
| GTGAAGATAGAGAAGCCTTG+TGG | 0.691864 | 4.1:-29532479 | MS.gene52116:CDS |
| CTGCCCACAAGACTGAAGCA+GGG | 0.691968 | 4.1:-29544020 | MS.gene52116:CDS |
| ATAGGGTAAGCCTCTATGTG+AGG | 0.692924 | 4.1:+29544499 | None:intergenic |
| AATCAGGTACTGGAACAGCA+GGG | 0.700220 | 4.1:+29544333 | None:intergenic |
| ATGCAGAGGATATGATTCGG+CGG | 0.708222 | 4.1:-29532449 | MS.gene52116:CDS |
| ATCCTCATTCTGTGTCACAG+TGG | 0.709329 | 4.1:+29544071 | None:intergenic |
| ACTATTATAATGCTTTGACA+GGG | 0.711480 | 4.1:-29543990 | MS.gene52116:CDS |
| GGCTGTAACCAATATCCCTG+AGG | 0.714417 | 4.1:+29544449 | None:intergenic |
| TCTCAAAATACCTTAAAACG+AGG | 0.724653 | 4.1:+29538288 | None:intergenic |
| CCAACAGGAGTAACACCAGG+AGG | 0.749977 | 4.1:+29544305 | None:intergenic |
| TAGTTAGGGTCAGAGCAAGG+CGG | 0.758647 | 4.1:+29544725 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 29532335 | 29547042 | 29532335 | ID=MS.gene52116 |
| chr4.1 | mRNA | 29532335 | 29547042 | 29532335 | ID=MS.gene52116.t1;Parent=MS.gene52116 |
| chr4.1 | exon | 29546931 | 29547042 | 29546931 | ID=MS.gene52116.t1.exon1;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29546931 | 29547042 | 29546931 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29546528 | 29546637 | 29546528 | ID=MS.gene52116.t1.exon2;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29546528 | 29546637 | 29546528 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29546286 | 29546441 | 29546286 | ID=MS.gene52116.t1.exon3;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29546286 | 29546441 | 29546286 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29544216 | 29544843 | 29544216 | ID=MS.gene52116.t1.exon4;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29544216 | 29544843 | 29544216 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29543951 | 29544114 | 29543951 | ID=MS.gene52116.t1.exon5;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29543951 | 29544114 | 29543951 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29543527 | 29543564 | 29543527 | ID=MS.gene52116.t1.exon6;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29543527 | 29543564 | 29543527 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29542390 | 29542468 | 29542390 | ID=MS.gene52116.t1.exon7;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29542390 | 29542468 | 29542390 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29540208 | 29540678 | 29540208 | ID=MS.gene52116.t1.exon8;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29540208 | 29540678 | 29540208 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29538299 | 29538382 | 29538299 | ID=MS.gene52116.t1.exon9;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29538299 | 29538382 | 29538299 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29537977 | 29538117 | 29537977 | ID=MS.gene52116.t1.exon10;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29537977 | 29538117 | 29537977 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29537757 | 29537863 | 29537757 | ID=MS.gene52116.t1.exon11;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29537757 | 29537863 | 29537757 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29535872 | 29535995 | 29535872 | ID=MS.gene52116.t1.exon12;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29535872 | 29535995 | 29535872 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29534151 | 29534294 | 29534151 | ID=MS.gene52116.t1.exon13;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29534151 | 29534294 | 29534151 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29533592 | 29533738 | 29533592 | ID=MS.gene52116.t1.exon14;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29533592 | 29533738 | 29533592 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29532873 | 29532995 | 29532873 | ID=MS.gene52116.t1.exon15;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29532873 | 29532995 | 29532873 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| chr4.1 | exon | 29532335 | 29532652 | 29532335 | ID=MS.gene52116.t1.exon16;Parent=MS.gene52116.t1 |
| chr4.1 | CDS | 29532335 | 29532652 | 29532335 | ID=cds.MS.gene52116.t1;Parent=MS.gene52116.t1 |
| Gene Sequence |
| Protein sequence |
