Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52294.t1 | XP_003624546.1 | 96.9 | 293 | 9 | 0 | 1 | 293 | 1 | 293 | 1.10E-171 | 612.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52294.t1 | Q8LNZ5 | 83.6 | 293 | 48 | 0 | 1 | 293 | 1 | 293 | 1.1e-153 | 543.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52294.t1 | Q2HRU7 | 96.9 | 293 | 9 | 0 | 1 | 293 | 1 | 293 | 7.6e-172 | 612.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene049783 | MS.gene52294 | PPI |
| MS.gene028653 | MS.gene52294 | PPI |
| MS.gene44431 | MS.gene52294 | PPI |
| MS.gene60167 | MS.gene52294 | PPI |
| MS.gene52294 | MS.gene43303 | PPI |
| MS.gene43849 | MS.gene52294 | PPI |
| MS.gene52294 | MS.gene011682 | PPI |
| MS.gene52294 | MS.gene011681 | PPI |
| MS.gene31221 | MS.gene52294 | PPI |
| MS.gene44430 | MS.gene52294 | PPI |
| MS.gene52294 | MS.gene45618 | PPI |
| MS.gene45619 | MS.gene52294 | PPI |
| MS.gene52294 | MS.gene09167 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52294.t1 | MTR_7g084750 | 96.928 | 293 | 9 | 0 | 1 | 293 | 1 | 293 | 0.0 | 594 |
| MS.gene52294.t1 | MTR_7g084770 | 85.666 | 293 | 42 | 0 | 1 | 293 | 1 | 293 | 0.0 | 542 |
| MS.gene52294.t1 | MTR_7g084760 | 84.642 | 293 | 45 | 0 | 1 | 293 | 1 | 293 | 0.0 | 536 |
| MS.gene52294.t1 | MTR_6g033055 | 79.181 | 293 | 61 | 0 | 1 | 293 | 1 | 293 | 0.0 | 501 |
| MS.gene52294.t1 | MTR_6g033085 | 78.498 | 293 | 63 | 0 | 1 | 293 | 1 | 293 | 0.0 | 501 |
| MS.gene52294.t1 | MTR_4g128410 | 52.577 | 291 | 125 | 4 | 1 | 288 | 1 | 281 | 1.49e-102 | 301 |
| MS.gene52294.t1 | MTR_2g089140 | 50.877 | 285 | 124 | 4 | 13 | 289 | 12 | 288 | 1.81e-102 | 301 |
| MS.gene52294.t1 | MTR_4g045853 | 49.310 | 290 | 131 | 4 | 6 | 287 | 7 | 288 | 1.49e-101 | 299 |
| MS.gene52294.t1 | MTR_3g084620 | 50.342 | 292 | 133 | 3 | 1 | 291 | 1 | 281 | 2.49e-100 | 295 |
| MS.gene52294.t1 | MTR_4g097700 | 52.529 | 257 | 120 | 2 | 32 | 287 | 69 | 324 | 2.17e-99 | 295 |
| MS.gene52294.t1 | MTR_4g097640 | 52.529 | 257 | 120 | 2 | 32 | 287 | 32 | 287 | 2.19e-99 | 293 |
| MS.gene52294.t1 | MTR_4g097670 | 52.529 | 257 | 120 | 2 | 32 | 287 | 32 | 287 | 2.19e-99 | 293 |
| MS.gene52294.t1 | MTR_3g084630 | 54.023 | 261 | 112 | 2 | 32 | 291 | 28 | 281 | 3.94e-99 | 292 |
| MS.gene52294.t1 | MTR_8g081040 | 54.864 | 257 | 109 | 3 | 32 | 287 | 31 | 281 | 5.95e-98 | 290 |
| MS.gene52294.t1 | MTR_7g093530 | 49.828 | 291 | 130 | 4 | 1 | 287 | 1 | 279 | 1.83e-96 | 286 |
| MS.gene52294.t1 | MTR_4g128580 | 53.030 | 264 | 112 | 3 | 32 | 287 | 26 | 285 | 1.09e-94 | 281 |
| MS.gene52294.t1 | MTR_1g105900 | 51.938 | 258 | 122 | 2 | 32 | 287 | 34 | 291 | 7.66e-94 | 280 |
| MS.gene52294.t1 | MTR_4g128570 | 54.475 | 257 | 101 | 4 | 32 | 287 | 31 | 272 | 1.38e-93 | 278 |
| MS.gene52294.t1 | MTR_1g105495 | 48.443 | 289 | 145 | 2 | 3 | 287 | 6 | 294 | 5.10e-93 | 278 |
| MS.gene52294.t1 | MTR_4g126920 | 51.341 | 261 | 118 | 3 | 32 | 287 | 25 | 281 | 4.87e-92 | 275 |
| MS.gene52294.t1 | MTR_6g088320 | 48.106 | 264 | 131 | 4 | 28 | 287 | 22 | 283 | 5.37e-92 | 275 |
| MS.gene52294.t1 | MTR_8g102220 | 51.341 | 261 | 107 | 3 | 32 | 291 | 29 | 270 | 2.34e-91 | 272 |
| MS.gene52294.t1 | MTR_4g128400 | 53.200 | 250 | 115 | 2 | 39 | 287 | 35 | 283 | 1.06e-90 | 271 |
| MS.gene52294.t1 | MTR_2g038860 | 50.566 | 265 | 118 | 3 | 32 | 287 | 26 | 286 | 2.69e-89 | 268 |
| MS.gene52294.t1 | MTR_2g038705 | 50.566 | 265 | 118 | 3 | 32 | 287 | 26 | 286 | 2.69e-89 | 268 |
| MS.gene52294.t1 | MTR_8g102910 | 46.429 | 280 | 144 | 4 | 12 | 287 | 2 | 279 | 3.91e-89 | 267 |
| MS.gene52294.t1 | MTR_2g089140 | 54.667 | 225 | 94 | 2 | 73 | 289 | 1 | 225 | 4.62e-89 | 265 |
| MS.gene52294.t1 | MTR_8g013055 | 47.710 | 262 | 136 | 1 | 26 | 287 | 25 | 285 | 1.09e-87 | 263 |
| MS.gene52294.t1 | MTR_4g128590 | 49.421 | 259 | 104 | 4 | 39 | 289 | 38 | 277 | 1.69e-83 | 253 |
| MS.gene52294.t1 | MTR_3g089540 | 48.594 | 249 | 115 | 3 | 50 | 293 | 48 | 288 | 2.40e-82 | 250 |
| MS.gene52294.t1 | MTR_5g091730 | 49.167 | 240 | 115 | 3 | 51 | 289 | 47 | 280 | 1.99e-79 | 243 |
| MS.gene52294.t1 | MTR_7g056090 | 44.170 | 283 | 141 | 6 | 7 | 288 | 16 | 282 | 1.49e-77 | 238 |
| MS.gene52294.t1 | MTR_2g049030 | 40.925 | 281 | 155 | 7 | 9 | 288 | 7 | 277 | 2.19e-67 | 211 |
| MS.gene52294.t1 | MTR_2g049130 | 43.825 | 251 | 134 | 7 | 29 | 277 | 26 | 271 | 7.79e-65 | 205 |
| MS.gene52294.t1 | MTR_5g029100 | 38.290 | 269 | 152 | 6 | 30 | 287 | 40 | 305 | 7.96e-62 | 199 |
| MS.gene52294.t1 | MTR_8g064180 | 38.189 | 254 | 146 | 5 | 45 | 288 | 59 | 311 | 1.08e-60 | 195 |
| MS.gene52294.t1 | MTR_1g033420 | 37.329 | 292 | 167 | 7 | 10 | 290 | 8 | 294 | 1.00e-59 | 193 |
| MS.gene52294.t1 | MTR_7g102260 | 37.500 | 264 | 148 | 7 | 32 | 287 | 37 | 291 | 1.40e-56 | 184 |
| MS.gene52294.t1 | MTR_8g090100 | 36.059 | 269 | 155 | 6 | 30 | 287 | 19 | 281 | 2.11e-56 | 184 |
| MS.gene52294.t1 | MTR_4g057450 | 36.842 | 266 | 151 | 7 | 30 | 287 | 48 | 304 | 3.39e-56 | 183 |
| MS.gene52294.t1 | MTR_1g063020 | 38.290 | 269 | 145 | 8 | 29 | 287 | 37 | 294 | 5.70e-56 | 182 |
| MS.gene52294.t1 | MTR_2g095800 | 36.552 | 290 | 161 | 10 | 12 | 287 | 29 | 309 | 6.09e-55 | 180 |
| MS.gene52294.t1 | MTR_5g029100 | 38.983 | 236 | 130 | 6 | 63 | 287 | 42 | 274 | 7.32e-54 | 178 |
| MS.gene52294.t1 | MTR_8g479340 | 33.333 | 186 | 63 | 3 | 103 | 287 | 10 | 135 | 8.26e-26 | 99.8 |
| MS.gene52294.t1 | MTR_8g032640 | 33.333 | 174 | 108 | 4 | 54 | 225 | 33 | 200 | 7.76e-16 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52294.t1 | AT5G13870 | 78.157 | 293 | 64 | 0 | 1 | 293 | 1 | 293 | 1.26e-180 | 499 |
| MS.gene52294.t1 | AT5G13870 | 78.157 | 293 | 64 | 0 | 1 | 293 | 1 | 293 | 1.26e-180 | 499 |
| MS.gene52294.t1 | AT5G13870 | 78.157 | 293 | 64 | 0 | 1 | 293 | 1 | 293 | 1.26e-180 | 499 |
| MS.gene52294.t1 | AT2G06850 | 75.932 | 295 | 68 | 1 | 1 | 292 | 1 | 295 | 1.12e-174 | 484 |
| MS.gene52294.t1 | AT2G06850 | 81.385 | 231 | 43 | 0 | 62 | 292 | 5 | 235 | 2.08e-147 | 413 |
| MS.gene52294.t1 | AT3G23730 | 52.431 | 288 | 123 | 4 | 10 | 291 | 11 | 290 | 2.57e-105 | 308 |
| MS.gene52294.t1 | AT5G65730 | 51.786 | 280 | 128 | 3 | 10 | 288 | 18 | 291 | 1.54e-104 | 306 |
| MS.gene52294.t1 | AT4G03210 | 53.409 | 264 | 119 | 2 | 32 | 293 | 29 | 290 | 7.16e-102 | 300 |
| MS.gene52294.t1 | AT4G14130 | 55.253 | 257 | 108 | 3 | 39 | 291 | 35 | 288 | 1.10e-101 | 299 |
| MS.gene52294.t1 | AT4G37800 | 50.362 | 276 | 133 | 3 | 13 | 287 | 17 | 289 | 5.88e-100 | 295 |
| MS.gene52294.t1 | AT5G57550 | 50.342 | 292 | 134 | 4 | 1 | 289 | 1 | 284 | 7.66e-97 | 286 |
| MS.gene52294.t1 | AT4G13090 | 50.534 | 281 | 132 | 4 | 11 | 288 | 13 | 289 | 1.32e-95 | 284 |
| MS.gene52294.t1 | AT5G57530 | 55.078 | 256 | 107 | 5 | 39 | 290 | 34 | 285 | 4.42e-95 | 282 |
| MS.gene52294.t1 | AT1G11545 | 50.896 | 279 | 127 | 4 | 15 | 287 | 25 | 299 | 8.23e-95 | 282 |
| MS.gene52294.t1 | AT5G57560 | 48.966 | 290 | 129 | 5 | 5 | 287 | 4 | 281 | 2.76e-94 | 280 |
| MS.gene52294.t1 | AT5G57540 | 52.852 | 263 | 116 | 4 | 32 | 290 | 26 | 284 | 1.19e-93 | 278 |
| MS.gene52294.t1 | AT4G30270 | 51.273 | 275 | 113 | 4 | 14 | 287 | 11 | 265 | 1.89e-93 | 278 |
| MS.gene52294.t1 | AT4G03210 | 51.172 | 256 | 120 | 2 | 40 | 293 | 10 | 262 | 2.45e-93 | 277 |
| MS.gene52294.t1 | AT4G28850 | 45.804 | 286 | 148 | 4 | 11 | 291 | 8 | 291 | 1.24e-91 | 274 |
| MS.gene52294.t1 | AT4G25810 | 51.504 | 266 | 119 | 4 | 28 | 287 | 22 | 283 | 5.25e-91 | 272 |
| MS.gene52294.t1 | AT3G25050 | 46.552 | 290 | 143 | 6 | 3 | 287 | 7 | 289 | 4.89e-90 | 270 |
| MS.gene52294.t1 | AT5G48070 | 53.333 | 240 | 106 | 3 | 49 | 287 | 47 | 281 | 2.80e-89 | 267 |
| MS.gene52294.t1 | AT2G14620 | 47.059 | 289 | 143 | 4 | 1 | 287 | 14 | 294 | 3.53e-89 | 268 |
| MS.gene52294.t1 | AT2G18800 | 45.791 | 297 | 140 | 5 | 9 | 287 | 3 | 296 | 1.90e-88 | 266 |
| MS.gene52294.t1 | AT4G25820 | 51.575 | 254 | 115 | 3 | 39 | 288 | 37 | 286 | 1.64e-87 | 263 |
| MS.gene52294.t1 | AT4G13080 | 44.599 | 287 | 154 | 4 | 2 | 288 | 11 | 292 | 3.53e-87 | 263 |
| MS.gene52294.t1 | AT4G30290 | 52.083 | 240 | 109 | 3 | 49 | 287 | 42 | 276 | 8.20e-83 | 251 |
| MS.gene52294.t1 | AT2G14620 | 52.423 | 227 | 106 | 2 | 63 | 287 | 18 | 244 | 1.51e-82 | 249 |
| MS.gene52294.t1 | AT1G65310 | 53.540 | 226 | 99 | 3 | 63 | 287 | 83 | 303 | 8.38e-82 | 249 |
| MS.gene52294.t1 | AT1G65310 | 53.540 | 226 | 99 | 3 | 63 | 287 | 61 | 281 | 1.03e-81 | 248 |
| MS.gene52294.t1 | AT4G30280 | 52.655 | 226 | 101 | 3 | 63 | 287 | 61 | 281 | 1.98e-81 | 247 |
| MS.gene52294.t1 | AT2G14620 | 52.968 | 219 | 101 | 2 | 71 | 287 | 15 | 233 | 1.37e-80 | 244 |
| MS.gene52294.t1 | AT3G44990 | 39.073 | 302 | 160 | 7 | 1 | 287 | 1 | 293 | 2.49e-63 | 201 |
| MS.gene52294.t1 | AT1G32170 | 35.640 | 289 | 174 | 5 | 6 | 287 | 10 | 293 | 2.09e-59 | 193 |
| MS.gene52294.t1 | AT1G14720 | 34.247 | 292 | 185 | 4 | 1 | 287 | 1 | 290 | 7.67e-58 | 189 |
| MS.gene52294.t1 | AT3G48580 | 38.372 | 258 | 143 | 5 | 30 | 287 | 32 | 273 | 7.34e-56 | 182 |
| MS.gene52294.t1 | AT1G10550 | 37.154 | 253 | 140 | 6 | 51 | 287 | 60 | 309 | 4.27e-55 | 181 |
| MS.gene52294.t1 | AT2G36870 | 39.431 | 246 | 134 | 5 | 52 | 289 | 61 | 299 | 1.88e-54 | 179 |
| MS.gene52294.t1 | AT2G01850 | 34.386 | 285 | 178 | 5 | 10 | 287 | 8 | 290 | 9.18e-54 | 178 |
| MS.gene52294.t1 | AT4G18990 | 36.508 | 252 | 141 | 4 | 54 | 287 | 62 | 312 | 2.77e-53 | 177 |
| MS.gene52294.t1 | AT3G48580 | 38.496 | 226 | 124 | 4 | 62 | 287 | 4 | 214 | 2.69e-49 | 163 |
| MS.gene52294.t1 | AT3G48580 | 32.946 | 258 | 137 | 6 | 30 | 287 | 32 | 253 | 1.87e-40 | 141 |
| MS.gene52294.t1 | AT4G18990 | 32.328 | 232 | 130 | 5 | 81 | 287 | 9 | 238 | 1.95e-40 | 142 |
| MS.gene52294.t1 | AT2G36870 | 44.068 | 118 | 58 | 2 | 52 | 161 | 61 | 178 | 1.18e-27 | 105 |
Find 68 sgRNAs with CRISPR-Local
Find 113 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATCATGTTCCGCATTT+TGG | 0.142294 | 7.3:+23879530 | None:intergenic |
| TGAAGGTATACAACAGTTTA+TGG | 0.206946 | 7.3:-23878668 | MS.gene52294:CDS |
| ACATTTGTTTGTAAAATATA+AGG | 0.260741 | 7.3:+23879472 | None:intergenic |
| TTCTTTGTTCTCTATTACCT+TGG | 0.292434 | 7.3:+23879437 | None:intergenic |
| ATCAGTACAGTAATTATAAA+TGG | 0.326064 | 7.3:+23878425 | None:intergenic |
| GTGCAAAACAAGGTAGAAAA+TGG | 0.352762 | 7.3:-23878521 | MS.gene52294:CDS |
| ACCATAGATACTCAGTTCTA+TGG | 0.355438 | 7.3:-23879384 | MS.gene52294:CDS |
| TTGTGGACAATATTCCAATC+AGG | 0.363427 | 7.3:-23878743 | MS.gene52294:CDS |
| TACAAAAGTTTCCACATTGA+TGG | 0.366093 | 7.3:-23878573 | MS.gene52294:CDS |
| AAACTGTTGTATACCTTCAT+TGG | 0.366710 | 7.3:+23878672 | None:intergenic |
| TCCATAGAACTGAGTATCTA+TGG | 0.368063 | 7.3:+23879383 | None:intergenic |
| ACCTTCATTGGTTGATCAAA+TGG | 0.372148 | 7.3:+23878684 | None:intergenic |
| TCTAAAGGATCATACTTGTT+TGG | 0.386128 | 7.3:-23879791 | MS.gene52294:CDS |
| CTCAATGGAAAAGGATAAAG+TGG | 0.398385 | 7.3:-23878464 | MS.gene52294:CDS |
| AGGCCGGTAGATGTACCTTT+TGG | 0.398803 | 7.3:-23880057 | MS.gene52294:CDS |
| GTTTATGGAATGCAGATGAT+TGG | 0.401396 | 7.3:-23878653 | MS.gene52294:CDS |
| ACAAGTATGATCCTTTAGAT+TGG | 0.403137 | 7.3:+23879795 | None:intergenic |
| TCCATTTGATCAACCAATGA+AGG | 0.419877 | 7.3:-23878685 | MS.gene52294:CDS |
| GAGAACAAAGAATATTTCTC+TGG | 0.421708 | 7.3:-23879426 | MS.gene52294:CDS |
| TCACGGTCTCTTCTACATTC+AGG | 0.438875 | 7.3:+23878378 | None:intergenic |
| CACATCAAATACTTTAATGA+AGG | 0.454409 | 7.3:-23880003 | MS.gene52294:CDS |
| ATGAACATAAAGATGGTTCC+TGG | 0.454798 | 7.3:-23879758 | MS.gene52294:CDS |
| GTACATCTACCGGCCTTCGC+GGG | 0.460487 | 7.3:+23880064 | None:intergenic |
| TTACAAACAAATGTGTTCAC+TGG | 0.460974 | 7.3:-23879463 | MS.gene52294:CDS |
| CAGCGCCACCCCGCGAAGGC+CGG | 0.466927 | 7.3:-23880073 | MS.gene52294:CDS |
| GTGACCTTGATGCTGCTCAA+TGG | 0.469367 | 7.3:-23878479 | MS.gene52294:CDS |
| TTTATGGAATGCAGATGATT+GGG | 0.473254 | 7.3:-23878652 | MS.gene52294:CDS |
| GGTACATCTACCGGCCTTCG+CGG | 0.484926 | 7.3:+23880063 | None:intergenic |
| TTGATGCTGCTCAATGGAAA+AGG | 0.491805 | 7.3:-23878473 | MS.gene52294:CDS |
| GGTACTGGCTTCCAATCTAA+AGG | 0.501003 | 7.3:-23879806 | MS.gene52294:CDS |
| ATGTATGTGTAGGTTCTTTG+TGG | 0.502520 | 7.3:-23878760 | MS.gene52294:intron |
| ATGGTTCCTGGTGATTCAGC+TGG | 0.504587 | 7.3:-23879746 | MS.gene52294:CDS |
| GGTACATAGTTTCGACCAAA+AGG | 0.510742 | 7.3:+23880042 | None:intergenic |
| CTTTCAGCGCCACCCCGCGA+AGG | 0.514234 | 7.3:-23880077 | MS.gene52294:CDS |
| GTCGAAACTATGTACCCACT+TGG | 0.530233 | 7.3:-23880035 | MS.gene52294:CDS |
| TCTTTATGTTCATGCTGAAG+TGG | 0.533369 | 7.3:+23879768 | None:intergenic |
| CTACATTCAGGAGGTATCTG+AGG | 0.537443 | 7.3:+23878390 | None:intergenic |
| CGACCAAAAGGTACATCTAC+CGG | 0.539669 | 7.3:+23880054 | None:intergenic |
| TGCAGATGATTGGGCAACAA+GGG | 0.548054 | 7.3:-23878643 | MS.gene52294:CDS |
| ATGCAGATGATTGGGCAACA+AGG | 0.548692 | 7.3:-23878644 | MS.gene52294:CDS |
| AATGTGTTCACTGGAGGCCA+AGG | 0.551160 | 7.3:-23879454 | MS.gene52294:CDS |
| TGTGGACAATATTCCAATCA+GGG | 0.551213 | 7.3:-23878742 | MS.gene52294:CDS |
| TCAAAAGCACCATTCATAGC+AGG | 0.555389 | 7.3:-23878597 | MS.gene52294:CDS |
| TACATCTACCGGCCTTCGCG+GGG | 0.559311 | 7.3:+23880065 | None:intergenic |
| CTTCAGCATGAACATAAAGA+TGG | 0.562772 | 7.3:-23879765 | MS.gene52294:CDS |
| GTGGATTAGAGAAAACTGAT+TGG | 0.570924 | 7.3:-23878620 | MS.gene52294:CDS |
| CGGGGTGGCGCTGAAAGCCG+CGG | 0.573582 | 7.3:+23880083 | None:intergenic |
| GATGATTGGGCAACAAGGGG+TGG | 0.574149 | 7.3:-23878639 | MS.gene52294:CDS |
| GCATCAAGGTCACGAAACTC+AGG | 0.577476 | 7.3:+23878489 | None:intergenic |
| CTTCTTCTTGATAAGTACAC+TGG | 0.587312 | 7.3:-23879967 | MS.gene52294:intron |
| ATCTACCGGCCTTCGCGGGG+TGG | 0.594450 | 7.3:+23880068 | None:intergenic |
| TAGAACTGAGTATCTATGGT+AGG | 0.601747 | 7.3:+23879387 | None:intergenic |
| GCAAAGTACTGTGCAAAACA+AGG | 0.603844 | 7.3:-23878531 | MS.gene52294:CDS |
| TACATTCAGGAGGTATCTGA+GGG | 0.605395 | 7.3:+23878391 | None:intergenic |
| TCGAAACTATGTACCCACTT+GGG | 0.605536 | 7.3:-23880034 | MS.gene52294:CDS |
| GCAGTTATCTTCCCAAAATG+CGG | 0.624739 | 7.3:-23879542 | MS.gene52294:intron |
| CAAAACAAGGTAGAAAATGG+TGG | 0.627129 | 7.3:-23878518 | MS.gene52294:CDS |
| CAAACAAATGTGTTCACTGG+AGG | 0.629079 | 7.3:-23879460 | MS.gene52294:CDS |
| TTTGTTGTGTCTAACGTCCG+CGG | 0.640803 | 7.3:-23880100 | MS.gene52294:CDS |
| CAAGGTCACGAAACTCAGGT+TGG | 0.649125 | 7.3:+23878493 | None:intergenic |
| GCAGATGATTGGGCAACAAG+GGG | 0.654510 | 7.3:-23878642 | MS.gene52294:CDS |
| ACTGTTCCAGCTGAATCACC+AGG | 0.657325 | 7.3:+23879740 | None:intergenic |
| ACAGTACTTTGCATTAACTG+AGG | 0.671350 | 7.3:+23878542 | None:intergenic |
| CGGTCTCTTCTACATTCAGG+AGG | 0.676223 | 7.3:+23878381 | None:intergenic |
| ATGTGATCAAAAGCCCAAGT+GGG | 0.680372 | 7.3:+23880021 | None:intergenic |
| TCAATGGAAAAGGATAAAGT+GGG | 0.694713 | 7.3:-23878463 | MS.gene52294:CDS |
| GATGTGATCAAAAGCCCAAG+TGG | 0.698619 | 7.3:+23880020 | None:intergenic |
| AGGTTTCACAACCATCAATG+TGG | 0.700769 | 7.3:+23878562 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTAATATATGTCTTAT+TGG | - | chr7.3:23878608-23878627 | MS.gene52294:CDS | 10.0% |
| !!! | TATTTATACAATTTTTGAAT+AGG | - | chr7.3:23878676-23878695 | MS.gene52294:CDS | 10.0% |
| !! | ACATTTGTTTGTAAAATATA+AGG | + | chr7.3:23879034-23879053 | None:intergenic | 15.0% |
| !! | ATCCCTAAATAAATAAATTT+CGG | + | chr7.3:23879432-23879451 | None:intergenic | 15.0% |
| !! | ATTAAGATTCTTTCTATATA+TGG | + | chr7.3:23879371-23879390 | None:intergenic | 15.0% |
| !! | TTAAGATTCTTTCTATATAT+GGG | + | chr7.3:23879370-23879389 | None:intergenic | 15.0% |
| !! | TTCTACATAGAAATTAATTA+AGG | + | chr7.3:23878578-23878597 | None:intergenic | 15.0% |
| !! | AAATTAATCATCTTTCATGA+AGG | + | chr7.3:23879574-23879593 | None:intergenic | 20.0% |
| !! | AATTAATCATCTTTCATGAA+GGG | + | chr7.3:23879573-23879592 | None:intergenic | 20.0% |
| !! | AGACCGAAATTTATTTATTT+AGG | - | chr7.3:23879426-23879445 | MS.gene52294:CDS | 20.0% |
| !! | ATCAAAACGTCATATAAATT+AGG | + | chr7.3:23879651-23879670 | None:intergenic | 20.0% |
| !! | ATCAGTACAGTAATTATAAA+TGG | + | chr7.3:23880081-23880100 | None:intergenic | 20.0% |
| !! | GACCGAAATTTATTTATTTA+GGG | - | chr7.3:23879427-23879446 | MS.gene52294:CDS | 20.0% |
| !!! | CTATCATAGTAATTTTTTAC+CGG | - | chr7.3:23879603-23879622 | MS.gene52294:intron | 20.0% |
| !!! | GAAATAGATTTTGAGTTTTT+GGG | - | chr7.3:23878992-23879011 | MS.gene52294:intron | 20.0% |
| !!! | TGAAATAGATTTTGAGTTTT+TGG | - | chr7.3:23878991-23879010 | MS.gene52294:intron | 20.0% |
| ! | AAACGTCATATAAATTAGGA+CGG | + | chr7.3:23879647-23879666 | None:intergenic | 25.0% |
| ! | AAATTCACATATGTATGTGT+AGG | - | chr7.3:23879733-23879752 | MS.gene52294:CDS | 25.0% |
| ! | CACATCAAATACTTTAATGA+AGG | - | chr7.3:23878500-23878519 | MS.gene52294:CDS | 25.0% |
| ! | TAGATAGAAAGATACTAACA+CGG | - | chr7.3:23879255-23879274 | MS.gene52294:intron | 25.0% |
| !! | AATTGATCCAATTTTGTAGT+GGG | - | chr7.3:23878864-23878883 | MS.gene52294:intron | 25.0% |
| !! | TACAATTTTTGAATAGGTAC+TGG | - | chr7.3:23878682-23878701 | MS.gene52294:CDS | 25.0% |
| !!! | GTTTTGAAGTCTTTCTTAAT+AGG | - | chr7.3:23879296-23879315 | MS.gene52294:intron | 25.0% |
| AAACTGTTGTATACCTTCAT+TGG | + | chr7.3:23879834-23879853 | None:intergenic | 30.0% | |
| AATTAACCCCACTACAAAAT+TGG | + | chr7.3:23878874-23878893 | None:intergenic | 30.0% | |
| ACAAGTATGATCCTTTAGAT+TGG | + | chr7.3:23878711-23878730 | None:intergenic | 30.0% | |
| CAAATCAGACAAAACTTATG+GGG | + | chr7.3:23879173-23879192 | None:intergenic | 30.0% | |
| CCAAATCAGACAAAACTTAT+GGG | + | chr7.3:23879174-23879193 | None:intergenic | 30.0% | |
| GAGAACAAAGAATATTTCTC+TGG | - | chr7.3:23879077-23879096 | MS.gene52294:intron | 30.0% | |
| TACAAAAGTTTCCACATTGA+TGG | - | chr7.3:23879930-23879949 | MS.gene52294:intron | 30.0% | |
| TCAATGGAAAAGGATAAAGT+GGG | - | chr7.3:23880040-23880059 | MS.gene52294:CDS | 30.0% | |
| TCTAAAGGATCATACTTGTT+TGG | - | chr7.3:23878712-23878731 | MS.gene52294:CDS | 30.0% | |
| TTACAAACAAATGTGTTCAC+TGG | - | chr7.3:23879040-23879059 | MS.gene52294:intron | 30.0% | |
| TTCAAAAACAGCAAGAAGTT+AGG | - | chr7.3:23879786-23879805 | MS.gene52294:CDS | 30.0% | |
| TTCTTTGTTCTCTATTACCT+TGG | + | chr7.3:23879069-23879088 | None:intergenic | 30.0% | |
| TTTATGGAATGCAGATGATT+GGG | - | chr7.3:23879851-23879870 | MS.gene52294:intron | 30.0% | |
| ! | ATTGATCCAATTTTGTAGTG+GGG | - | chr7.3:23878865-23878884 | MS.gene52294:intron | 30.0% |
| ! | GAATTGATCCAATTTTGTAG+TGG | - | chr7.3:23878863-23878882 | MS.gene52294:intron | 30.0% |
| ! | TGAAGGTATACAACAGTTTA+TGG | - | chr7.3:23879835-23879854 | MS.gene52294:intron | 30.0% |
| ! | TTTTGTGCATACATAGAAAG+CGG | + | chr7.3:23878791-23878810 | None:intergenic | 30.0% |
| !! | CCATAAGTTTTGTCTGATTT+GGG | - | chr7.3:23879172-23879191 | MS.gene52294:intron | 30.0% |
| ACAGTACTTTGCATTAACTG+AGG | + | chr7.3:23879964-23879983 | None:intergenic | 35.0% | |
| ACCATAGATACTCAGTTCTA+TGG | - | chr7.3:23879119-23879138 | MS.gene52294:intron | 35.0% | |
| ATAGGATGACTAAAATCGCA+TGG | - | chr7.3:23879314-23879333 | MS.gene52294:intron | 35.0% | |
| ATGAACATAAAGATGGTTCC+TGG | - | chr7.3:23878745-23878764 | MS.gene52294:CDS | 35.0% | |
| ATGTATGTGTAGGTTCTTTG+TGG | - | chr7.3:23879743-23879762 | MS.gene52294:CDS | 35.0% | |
| CAAAACAAGGTAGAAAATGG+TGG | - | chr7.3:23879985-23880004 | MS.gene52294:CDS | 35.0% | |
| CCCAAATCAGACAAAACTTA+TGG | + | chr7.3:23879175-23879194 | None:intergenic | 35.0% | |
| CTCAATGGAAAAGGATAAAG+TGG | - | chr7.3:23880039-23880058 | MS.gene52294:CDS | 35.0% | |
| CTTCAGCATGAACATAAAGA+TGG | - | chr7.3:23878738-23878757 | MS.gene52294:CDS | 35.0% | |
| CTTCTTCTTGATAAGTACAC+TGG | - | chr7.3:23878536-23878555 | MS.gene52294:CDS | 35.0% | |
| GTGCAAAACAAGGTAGAAAA+TGG | - | chr7.3:23879982-23880001 | MS.gene52294:CDS | 35.0% | |
| GTTTATGGAATGCAGATGAT+TGG | - | chr7.3:23879850-23879869 | MS.gene52294:intron | 35.0% | |
| TAGAACTGAGTATCTATGGT+AGG | + | chr7.3:23879119-23879138 | None:intergenic | 35.0% | |
| TCCATAGAACTGAGTATCTA+TGG | + | chr7.3:23879123-23879142 | None:intergenic | 35.0% | |
| TCCATTTGATCAACCAATGA+AGG | - | chr7.3:23879818-23879837 | MS.gene52294:intron | 35.0% | |
| TCTTTATGTTCATGCTGAAG+TGG | + | chr7.3:23878738-23878757 | None:intergenic | 35.0% | |
| TGTGGACAATATTCCAATCA+GGG | - | chr7.3:23879761-23879780 | MS.gene52294:CDS | 35.0% | |
| TTGTGGACAATATTCCAATC+AGG | - | chr7.3:23879760-23879779 | MS.gene52294:CDS | 35.0% | |
| ! | ACCTTCATTGGTTGATCAAA+TGG | + | chr7.3:23879822-23879841 | None:intergenic | 35.0% |
| ! | CTTTTGTAACCTGCTATGAA+TGG | + | chr7.3:23879918-23879937 | None:intergenic | 35.0% |
| ! | TATCTATGGTAGGCTTTTGT+AGG | + | chr7.3:23879109-23879128 | None:intergenic | 35.0% |
| ! | TTCATCATGTTCCGCATTTT+GGG | + | chr7.3:23878975-23878994 | None:intergenic | 35.0% |
| ! | TTTCATCATGTTCCGCATTT+TGG | + | chr7.3:23878976-23878995 | None:intergenic | 35.0% |
| !! | CCCATAAGTTTTGTCTGATT+TGG | - | chr7.3:23879171-23879190 | MS.gene52294:intron | 35.0% |
| !! | GTGGATTAGAGAAAACTGAT+TGG | - | chr7.3:23879883-23879902 | MS.gene52294:intron | 35.0% |
| AGGTTTCACAACCATCAATG+TGG | + | chr7.3:23879944-23879963 | None:intergenic | 40.0% | |
| ATGACTAAAATCGCATGGCT+AGG | - | chr7.3:23879319-23879338 | MS.gene52294:intron | 40.0% | |
| ATGTGATCAAAAGCCCAAGT+GGG | + | chr7.3:23878485-23878504 | None:intergenic | 40.0% | |
| CAAAACTCCAAGTCAAAGCA+TGG | + | chr7.3:23879282-23879301 | None:intergenic | 40.0% | |
| CAAACAAATGTGTTCACTGG+AGG | - | chr7.3:23879043-23879062 | MS.gene52294:intron | 40.0% | |
| GCAGTTATCTTCCCAAAATG+CGG | - | chr7.3:23878961-23878980 | MS.gene52294:intron | 40.0% | |
| GGTACATAGTTTCGACCAAA+AGG | + | chr7.3:23878464-23878483 | None:intergenic | 40.0% | |
| TCGAAACTATGTACCCACTT+GGG | - | chr7.3:23878469-23878488 | MS.gene52294:CDS | 40.0% | |
| TTGATGCTGCTCAATGGAAA+AGG | - | chr7.3:23880030-23880049 | MS.gene52294:CDS | 40.0% | |
| ! | GATAAGTTGAAACGTGAGTC+AGG | - | chr7.3:23879469-23879488 | MS.gene52294:CDS | 40.0% |
| ! | TTTTCCATTGAGCAGCATCA+AGG | + | chr7.3:23880031-23880050 | None:intergenic | 40.0% |
| !! | GAGAAAGTACTATGATGGAC+CGG | + | chr7.3:23879625-23879644 | None:intergenic | 40.0% |
| !! | GCAAAGTACTGTGCAAAACA+AGG | - | chr7.3:23879972-23879991 | MS.gene52294:CDS | 40.0% |
| !! | TACATTCAGGAGGTATCTGA+GGG | + | chr7.3:23880115-23880134 | None:intergenic | 40.0% |
| !! | TCAAAAGCACCATTCATAGC+AGG | - | chr7.3:23879906-23879925 | MS.gene52294:intron | 40.0% |
| !!! | CTGTTTTTGAACACCCTGAT+TGG | + | chr7.3:23879777-23879796 | None:intergenic | 40.0% |
| !!! | GAGTTTTTGGGTAACAGAAC+AGG | - | chr7.3:23879004-23879023 | MS.gene52294:intron | 40.0% |
| ATGCAGATGATTGGGCAACA+AGG | - | chr7.3:23879859-23879878 | MS.gene52294:intron | 45.0% | |
| GATGTGATCAAAAGCCCAAG+TGG | + | chr7.3:23878486-23878505 | None:intergenic | 45.0% | |
| GTCGAAACTATGTACCCACT+TGG | - | chr7.3:23878468-23878487 | MS.gene52294:CDS | 45.0% | |
| TCACGGTCTCTTCTACATTC+AGG | + | chr7.3:23880128-23880147 | None:intergenic | 45.0% | |
| TGCAGATGATTGGGCAACAA+GGG | - | chr7.3:23879860-23879879 | MS.gene52294:intron | 45.0% | |
| ! | CGACCAAAAGGTACATCTAC+CGG | + | chr7.3:23878452-23878471 | None:intergenic | 45.0% |
| ! | TTTGTTGTGTCTAACGTCCG+CGG | - | chr7.3:23878403-23878422 | MS.gene52294:CDS | 45.0% |
| !! | CTACATTCAGGAGGTATCTG+AGG | + | chr7.3:23880116-23880135 | None:intergenic | 45.0% |
| !! | GGACGGAGAAAGTACTATGA+TGG | + | chr7.3:23879630-23879649 | None:intergenic | 45.0% |
| !! | GGTACTGGCTTCCAATCTAA+AGG | - | chr7.3:23878697-23878716 | MS.gene52294:CDS | 45.0% |
| AATGTGTTCACTGGAGGCCA+AGG | - | chr7.3:23879049-23879068 | MS.gene52294:intron | 50.0% | |
| ACACGGTCCATGCTTTGACT+TGG | - | chr7.3:23879272-23879291 | MS.gene52294:intron | 50.0% | |
| ACTGTTCCAGCTGAATCACC+AGG | + | chr7.3:23878766-23878785 | None:intergenic | 50.0% | |
| ATGGTTCCTGGTGATTCAGC+TGG | - | chr7.3:23878757-23878776 | MS.gene52294:intron | 50.0% | |
| CAAGGTCACGAAACTCAGGT+TGG | + | chr7.3:23880013-23880032 | None:intergenic | 50.0% | |
| CGGTCTCTTCTACATTCAGG+AGG | + | chr7.3:23880125-23880144 | None:intergenic | 50.0% | |
| GCAGATGATTGGGCAACAAG+GGG | - | chr7.3:23879861-23879880 | MS.gene52294:intron | 50.0% | |
| GCATCAAGGTCACGAAACTC+AGG | + | chr7.3:23880017-23880036 | None:intergenic | 50.0% | |
| GGGATATCACGTGCACAACA+TGG | - | chr7.3:23879447-23879466 | MS.gene52294:CDS | 50.0% | |
| GTGACCTTGATGCTGCTCAA+TGG | - | chr7.3:23880024-23880043 | MS.gene52294:CDS | 50.0% | |
| ! | AGGCCGGTAGATGTACCTTT+TGG | - | chr7.3:23878446-23878465 | MS.gene52294:CDS | 50.0% |
| ! | TGGGGTTTTCGTCGTGGATT+TGG | - | chr7.3:23878372-23878391 | MS.gene52294:CDS | 50.0% |
| GATGATTGGGCAACAAGGGG+TGG | - | chr7.3:23879864-23879883 | MS.gene52294:intron | 55.0% | |
| GGTACATCTACCGGCCTTCG+CGG | + | chr7.3:23878443-23878462 | None:intergenic | 60.0% | |
| GTACATCTACCGGCCTTCGC+GGG | + | chr7.3:23878442-23878461 | None:intergenic | 60.0% | |
| TACATCTACCGGCCTTCGCG+GGG | + | chr7.3:23878441-23878460 | None:intergenic | 60.0% | |
| ATCTACCGGCCTTCGCGGGG+TGG | + | chr7.3:23878438-23878457 | None:intergenic | 70.0% | |
| CTTTCAGCGCCACCCCGCGA+AGG | - | chr7.3:23878426-23878445 | MS.gene52294:CDS | 70.0% | |
| ! | CGGGGTGGCGCTGAAAGCCG+CGG | + | chr7.3:23878423-23878442 | None:intergenic | 75.0% |
| ! | CAGCGCCACCCCGCGAAGGC+CGG | - | chr7.3:23878430-23878449 | MS.gene52294:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 23878371 | 23880154 | 23878371 | ID=MS.gene52294 |
| chr7.3 | mRNA | 23878371 | 23880154 | 23878371 | ID=MS.gene52294.t1;Parent=MS.gene52294 |
| chr7.3 | exon | 23879968 | 23880154 | 23879968 | ID=MS.gene52294.t1.exon1;Parent=MS.gene52294.t1 |
| chr7.3 | CDS | 23879968 | 23880154 | 23879968 | ID=cds.MS.gene52294.t1;Parent=MS.gene52294.t1 |
| chr7.3 | exon | 23879727 | 23879827 | 23879727 | ID=MS.gene52294.t1.exon2;Parent=MS.gene52294.t1 |
| chr7.3 | CDS | 23879727 | 23879827 | 23879727 | ID=cds.MS.gene52294.t1;Parent=MS.gene52294.t1 |
| chr7.3 | exon | 23879367 | 23879560 | 23879367 | ID=MS.gene52294.t1.exon3;Parent=MS.gene52294.t1 |
| chr7.3 | CDS | 23879367 | 23879560 | 23879367 | ID=cds.MS.gene52294.t1;Parent=MS.gene52294.t1 |
| chr7.3 | exon | 23878371 | 23878770 | 23878371 | ID=MS.gene52294.t1.exon4;Parent=MS.gene52294.t1 |
| chr7.3 | CDS | 23878371 | 23878770 | 23878371 | ID=cds.MS.gene52294.t1;Parent=MS.gene52294.t1 |
| Gene Sequence |
| Protein sequence |
