Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52772.t1 | XP_013462271.1 | 97.3 | 222 | 6 | 0 | 1 | 222 | 1 | 222 | 2.20E-113 | 418.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52772.t1 | I3SCR3 | 97.3 | 222 | 6 | 0 | 1 | 222 | 1 | 222 | 1.6e-113 | 418.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049989 | MS.gene52772 | 0.802202 | 6.27E-49 | -1.69E-46 |
| MS.gene051816 | MS.gene52772 | 0.837316 | 5.59E-57 | -1.69E-46 |
| MS.gene051955 | MS.gene52772 | 0.837524 | 4.94E-57 | -1.69E-46 |
| MS.gene052308 | MS.gene52772 | 0.823737 | 1.18E-53 | -1.69E-46 |
| MS.gene052367 | MS.gene52772 | 0.859771 | 3.28E-63 | -1.69E-46 |
| MS.gene053173 | MS.gene52772 | 0.807499 | 4.91E-50 | -1.69E-46 |
| MS.gene054551 | MS.gene52772 | 0.82628 | 2.96E-54 | -1.69E-46 |
| MS.gene054593 | MS.gene52772 | 0.813445 | 2.55E-51 | -1.69E-46 |
| MS.gene054662 | MS.gene52772 | 0.804073 | 2.57E-49 | -1.69E-46 |
| MS.gene055308 | MS.gene52772 | 0.833601 | 4.86E-56 | -1.69E-46 |
| MS.gene055578 | MS.gene52772 | 0.838097 | 3.52E-57 | -1.69E-46 |
| MS.gene055579 | MS.gene52772 | 0.801466 | 8.88E-49 | -1.69E-46 |
| MS.gene055957 | MS.gene52772 | 0.836454 | 9.27E-57 | -1.69E-46 |
| MS.gene056041 | MS.gene52772 | 0.865468 | 5.78E-65 | -1.69E-46 |
| MS.gene05640 | MS.gene52772 | 0.844355 | 7.96E-59 | -1.69E-46 |
| MS.gene057458 | MS.gene52772 | 0.835774 | 1.38E-56 | -1.69E-46 |
| MS.gene058013 | MS.gene52772 | 0.809197 | 2.13E-50 | -1.69E-46 |
| MS.gene058128 | MS.gene52772 | 0.810884 | 9.23E-51 | -1.69E-46 |
| MS.gene058853 | MS.gene52772 | 0.828048 | 1.12E-54 | -1.69E-46 |
| MS.gene059553 | MS.gene52772 | 0.813529 | 2.44E-51 | -1.69E-46 |
| MS.gene060473 | MS.gene52772 | 0.842083 | 3.21E-58 | -1.69E-46 |
| MS.gene060565 | MS.gene52772 | 0.836834 | 7.42E-57 | -1.69E-46 |
| MS.gene06093 | MS.gene52772 | 0.823581 | 1.28E-53 | -1.69E-46 |
| MS.gene061099 | MS.gene52772 | 0.853357 | 2.52E-61 | -1.69E-46 |
| MS.gene061260 | MS.gene52772 | 0.842577 | 2.38E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52772.t1 | MTR_3g115300 | 97.297 | 222 | 6 | 0 | 1 | 222 | 1 | 222 | 1.18e-158 | 438 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52772.t1 | AT5G42330 | 47.208 | 197 | 83 | 10 | 6 | 194 | 2 | 185 | 1.01e-36 | 128 |
Find 51 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAACAGAACACCAGATTT+AGG | 0.204443 | 3.1:-91228396 | None:intergenic |
| GAAGCAATTGTGCGGTATTC+AGG | 0.265276 | 3.1:-91228288 | None:intergenic |
| TCGGTCGCGGTGGTCGGAGG+TGG | 0.289193 | 3.1:-91228042 | None:intergenic |
| TGGTCGGAGGTGGGATTTCA+TGG | 0.291163 | 3.1:-91228032 | None:intergenic |
| GAAACAGAACACCAGATTTA+GGG | 0.318085 | 3.1:-91228395 | None:intergenic |
| CCAGATCCGGAAGAAATTAA+TGG | 0.326179 | 3.1:+91228357 | MS.gene52772:CDS |
| CCATTAATTTCTTCCGGATC+TGG | 0.333300 | 3.1:-91228357 | None:intergenic |
| TTTAACCAAGTCTCGGAAAC+TGG | 0.342643 | 3.1:+91228245 | MS.gene52772:CDS |
| AGATTTAGGGTTACAAGAAT+TGG | 0.342948 | 3.1:-91228382 | None:intergenic |
| CTTGGTGTTACTGTTGAATC+TGG | 0.346379 | 3.1:+91228549 | MS.gene52772:CDS |
| TTGGTACCATTAATTTCTTC+CGG | 0.348296 | 3.1:-91228363 | None:intergenic |
| ATGATGAGTATTCTGCAAAA+AGG | 0.356084 | 3.1:+91228433 | MS.gene52772:CDS |
| CGAAGACGCATCGGAGACTT+AGG | 0.358488 | 3.1:-91228067 | None:intergenic |
| CGGTATTCAGGTCGTAGTTC+AGG | 0.361330 | 3.1:-91228276 | None:intergenic |
| AATAATCATCATAATGTTCT+TGG | 0.387259 | 3.1:+91228531 | MS.gene52772:CDS |
| AATTCTTGTAACCCTAAATC+TGG | 0.396987 | 3.1:+91228384 | MS.gene52772:CDS |
| AGGATGGTTCGTGACGAGTT+TGG | 0.412043 | 3.1:+91228330 | MS.gene52772:CDS |
| CTGAATTTATTGCTTTATCA+AGG | 0.412653 | 3.1:+91228310 | MS.gene52772:CDS |
| AAGAATACTGCAAGAAAACT+TGG | 0.439698 | 3.1:+91228576 | MS.gene52772:CDS |
| CCCTCTCTTCAAACTCCACC+AGG | 0.453304 | 3.1:+91228112 | MS.gene52772:CDS |
| GAGAGGTTGAAGAGAAAGAA+AGG | 0.462625 | 3.1:+91228459 | MS.gene52772:CDS |
| TAGGGATCGGTCGCGGTGGT+CGG | 0.470489 | 3.1:-91228048 | None:intergenic |
| TTGAACCAGTTTCCGAGACT+TGG | 0.470567 | 3.1:-91228250 | None:intergenic |
| CGGTCGCGGTGGTCGGAGGT+GGG | 0.481693 | 3.1:-91228041 | None:intergenic |
| ATTTATTGCTTTATCAAGGA+TGG | 0.497038 | 3.1:+91228314 | MS.gene52772:CDS |
| AATAAGAAACCTCCTCTAGC+TGG | 0.510440 | 3.1:+91228684 | MS.gene52772:CDS |
| CGAGTTTGGAAAACCAGATC+CGG | 0.511115 | 3.1:+91228344 | MS.gene52772:CDS |
| TACTAGTATTAAGCAAACTA+AGG | 0.520435 | 3.1:+91228506 | MS.gene52772:CDS |
| GTTGGAGCATCGAGAAGAGT+AGG | 0.523400 | 3.1:+91228747 | MS.gene52772:CDS |
| ACTGTTGGTGAGAAGAAAGT+TGG | 0.529021 | 3.1:+91228729 | MS.gene52772:CDS |
| GGATCGGTCGCGGTGGTCGG+AGG | 0.529657 | 3.1:-91228045 | None:intergenic |
| CCTGGTGGAGTTTGAAGAGA+GGG | 0.535099 | 3.1:-91228112 | None:intergenic |
| CATGCTTAGAAGCATGACTA+AGG | 0.538545 | 3.1:+91228659 | MS.gene52772:CDS |
| CAGGTTCTTTAACCAAGTCT+CGG | 0.545491 | 3.1:+91228238 | MS.gene52772:intron |
| TTCTGCTGCTTATTCTGCTG+AGG | 0.545660 | 3.1:+91228617 | MS.gene52772:CDS |
| TTTGATAAATCTGTTACTGT+TGG | 0.555181 | 3.1:+91228714 | MS.gene52772:CDS |
| ACCTGGTGGAGTTTGAAGAG+AGG | 0.555239 | 3.1:-91228113 | None:intergenic |
| GAAGACGCATCGGAGACTTA+GGG | 0.556071 | 3.1:-91228066 | None:intergenic |
| TCAAATCTTGAACCAGCTAG+AGG | 0.605228 | 3.1:-91228696 | None:intergenic |
| CGCATCGGAGACTTAGGGAT+CGG | 0.618390 | 3.1:-91228061 | None:intergenic |
| ATGCTTCTAAGCATGTACCT+TGG | 0.620137 | 3.1:-91228651 | None:intergenic |
| AAGAGAGGGAGAGTCAAGAA+CGG | 0.623596 | 3.1:-91228098 | None:intergenic |
| GTTCGAGTTCGAAGACGCAT+CGG | 0.624113 | 3.1:-91228076 | None:intergenic |
| GGAGACTTAGGGATCGGTCG+CGG | 0.628168 | 3.1:-91228055 | None:intergenic |
| AATCTTGAACCAGCTAGAGG+AGG | 0.634666 | 3.1:-91228693 | None:intergenic |
| CTGAGGTTAGTGAAACTCCA+AGG | 0.641822 | 3.1:+91228634 | MS.gene52772:CDS |
| GACTTAGGGATCGGTCGCGG+TGG | 0.645567 | 3.1:-91228052 | None:intergenic |
| GAAGAGAAAGAAAGGAGTCA+CGG | 0.667345 | 3.1:+91228467 | MS.gene52772:CDS |
| TAAATTCAGAAGCAATTGTG+CGG | 0.689492 | 3.1:-91228296 | None:intergenic |
| TCTGGTGTTCTGTTTCAGAG+AGG | 0.698623 | 3.1:+91228402 | MS.gene52772:CDS |
| GAGAAAGAAAGGAGTCACGG+TGG | 0.729465 | 3.1:+91228470 | MS.gene52772:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATCATCATAATGTTCT+TGG | + | chr3.1:91228531-91228550 | MS.gene52772:CDS | 20.0% |
| ! | ATTTATTGCTTTATCAAGGA+TGG | + | chr3.1:91228314-91228333 | MS.gene52772:CDS | 25.0% |
| ! | CTGAATTTATTGCTTTATCA+AGG | + | chr3.1:91228310-91228329 | MS.gene52772:CDS | 25.0% |
| ! | GAAATAAGATTATAAGAACC+TGG | - | chr3.1:91228133-91228152 | None:intergenic | 25.0% |
| ! | TACTAGTATTAAGCAAACTA+AGG | + | chr3.1:91228506-91228525 | MS.gene52772:CDS | 25.0% |
| ! | TTTGATAAATCTGTTACTGT+TGG | + | chr3.1:91228714-91228733 | MS.gene52772:CDS | 25.0% |
| AAGAATACTGCAAGAAAACT+TGG | + | chr3.1:91228576-91228595 | MS.gene52772:CDS | 30.0% | |
| AATTCTTGTAACCCTAAATC+TGG | + | chr3.1:91228384-91228403 | MS.gene52772:CDS | 30.0% | |
| ATAAGATTATAAGAACCTGG+TGG | - | chr3.1:91228130-91228149 | None:intergenic | 30.0% | |
| ATGATGAGTATTCTGCAAAA+AGG | + | chr3.1:91228433-91228452 | MS.gene52772:CDS | 30.0% | |
| CCACAAACAAAAAATGTCAA+TGG | - | chr3.1:91228203-91228222 | None:intergenic | 30.0% | |
| GTGGATTTGAAAATTGAAAC+AGG | + | chr3.1:91228219-91228238 | MS.gene52772:intron | 30.0% | |
| TAAATTCAGAAGCAATTGTG+CGG | - | chr3.1:91228299-91228318 | None:intergenic | 30.0% | |
| TTGGTACCATTAATTTCTTC+CGG | - | chr3.1:91228366-91228385 | None:intergenic | 30.0% | |
| ! | AGATTTAGGGTTACAAGAAT+TGG | - | chr3.1:91228385-91228404 | None:intergenic | 30.0% |
| !!! | CCATTGACATTTTTTGTTTG+TGG | + | chr3.1:91228200-91228219 | MS.gene52772:intron | 30.0% |
| GAAACAGAACACCAGATTTA+GGG | - | chr3.1:91228398-91228417 | None:intergenic | 35.0% | |
| TGAAACAGAACACCAGATTT+AGG | - | chr3.1:91228399-91228418 | None:intergenic | 35.0% | |
| AATAAGAAACCTCCTCTAGC+TGG | + | chr3.1:91228684-91228703 | MS.gene52772:CDS | 40.0% | |
| ATGCTTCTAAGCATGTACCT+TGG | - | chr3.1:91228654-91228673 | None:intergenic | 40.0% | |
| ATTCTGCAAAAAGGAACGAG+AGG | + | chr3.1:91228442-91228461 | MS.gene52772:CDS | 40.0% | |
| CAGGTTCTTTAACCAAGTCT+CGG | + | chr3.1:91228238-91228257 | MS.gene52772:intron | 40.0% | |
| CCAGATCCGGAAGAAATTAA+TGG | + | chr3.1:91228357-91228376 | MS.gene52772:CDS | 40.0% | |
| CCATTAATTTCTTCCGGATC+TGG | - | chr3.1:91228360-91228379 | None:intergenic | 40.0% | |
| GAAGAGAAAGAAAGGAGTCA+CGG | + | chr3.1:91228467-91228486 | MS.gene52772:CDS | 40.0% | |
| GAGAGGTTGAAGAGAAAGAA+AGG | + | chr3.1:91228459-91228478 | MS.gene52772:CDS | 40.0% | |
| TCAAATCTTGAACCAGCTAG+AGG | - | chr3.1:91228699-91228718 | None:intergenic | 40.0% | |
| TTTAACCAAGTCTCGGAAAC+TGG | + | chr3.1:91228245-91228264 | MS.gene52772:CDS | 40.0% | |
| ! | ACTGTTGGTGAGAAGAAAGT+TGG | + | chr3.1:91228729-91228748 | MS.gene52772:CDS | 40.0% |
| ! | CATGCTTAGAAGCATGACTA+AGG | + | chr3.1:91228659-91228678 | MS.gene52772:CDS | 40.0% |
| !! | CTTGGTGTTACTGTTGAATC+TGG | + | chr3.1:91228549-91228568 | MS.gene52772:CDS | 40.0% |
| AAGAGAGGGAGAGTCAAGAA+CGG | - | chr3.1:91228101-91228120 | None:intergenic | 45.0% | |
| AATCTTGAACCAGCTAGAGG+AGG | - | chr3.1:91228696-91228715 | None:intergenic | 45.0% | |
| CGAGTTTGGAAAACCAGATC+CGG | + | chr3.1:91228344-91228363 | MS.gene52772:CDS | 45.0% | |
| CTGAGGTTAGTGAAACTCCA+AGG | + | chr3.1:91228634-91228653 | MS.gene52772:CDS | 45.0% | |
| GAAGCAATTGTGCGGTATTC+AGG | - | chr3.1:91228291-91228310 | None:intergenic | 45.0% | |
| TCTGGTGTTCTGTTTCAGAG+AGG | + | chr3.1:91228402-91228421 | MS.gene52772:CDS | 45.0% | |
| TTGAACCAGTTTCCGAGACT+TGG | - | chr3.1:91228253-91228272 | None:intergenic | 45.0% | |
| ! | TTCTGCTGCTTATTCTGCTG+AGG | + | chr3.1:91228617-91228636 | MS.gene52772:CDS | 45.0% |
| ACCTGGTGGAGTTTGAAGAG+AGG | - | chr3.1:91228116-91228135 | None:intergenic | 50.0% | |
| AGGATGGTTCGTGACGAGTT+TGG | + | chr3.1:91228330-91228349 | MS.gene52772:CDS | 50.0% | |
| CCTGGTGGAGTTTGAAGAGA+GGG | - | chr3.1:91228115-91228134 | None:intergenic | 50.0% | |
| CGGTATTCAGGTCGTAGTTC+AGG | - | chr3.1:91228279-91228298 | None:intergenic | 50.0% | |
| GAAGACGCATCGGAGACTTA+GGG | - | chr3.1:91228069-91228088 | None:intergenic | 50.0% | |
| GAGAAAGAAAGGAGTCACGG+TGG | + | chr3.1:91228470-91228489 | MS.gene52772:CDS | 50.0% | |
| ! | GTTCGAGTTCGAAGACGCAT+CGG | - | chr3.1:91228079-91228098 | None:intergenic | 50.0% |
| !! | GTTGGAGCATCGAGAAGAGT+AGG | + | chr3.1:91228747-91228766 | MS.gene52772:CDS | 50.0% |
| CCCTCTCTTCAAACTCCACC+AGG | + | chr3.1:91228112-91228131 | MS.gene52772:CDS | 55.0% | |
| CGAAGACGCATCGGAGACTT+AGG | - | chr3.1:91228070-91228089 | None:intergenic | 55.0% | |
| CGCATCGGAGACTTAGGGAT+CGG | - | chr3.1:91228064-91228083 | None:intergenic | 55.0% | |
| TGGTCGGAGGTGGGATTTCA+TGG | - | chr3.1:91228035-91228054 | None:intergenic | 55.0% | |
| GGAGACTTAGGGATCGGTCG+CGG | - | chr3.1:91228058-91228077 | None:intergenic | 60.0% | |
| GACTTAGGGATCGGTCGCGG+TGG | - | chr3.1:91228055-91228074 | None:intergenic | 65.0% | |
| TAGGGATCGGTCGCGGTGGT+CGG | - | chr3.1:91228051-91228070 | None:intergenic | 65.0% | |
| CGGTCGCGGTGGTCGGAGGT+GGG | - | chr3.1:91228044-91228063 | None:intergenic | 75.0% | |
| GGATCGGTCGCGGTGGTCGG+AGG | - | chr3.1:91228048-91228067 | None:intergenic | 75.0% | |
| TCGGTCGCGGTGGTCGGAGG+TGG | - | chr3.1:91228045-91228064 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 91228013 | 91228788 | 91228013 | ID=MS.gene52772 |
| chr3.1 | mRNA | 91228013 | 91228788 | 91228013 | ID=MS.gene52772.t1;Parent=MS.gene52772 |
| chr3.1 | exon | 91228013 | 91228133 | 91228013 | ID=MS.gene52772.t1.exon1;Parent=MS.gene52772.t1 |
| chr3.1 | CDS | 91228013 | 91228133 | 91228013 | ID=cds.MS.gene52772.t1;Parent=MS.gene52772.t1 |
| chr3.1 | exon | 91228241 | 91228788 | 91228241 | ID=MS.gene52772.t1.exon2;Parent=MS.gene52772.t1 |
| chr3.1 | CDS | 91228241 | 91228788 | 91228241 | ID=cds.MS.gene52772.t1;Parent=MS.gene52772.t1 |
| Gene Sequence |
| Protein sequence |
