Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59021.t1 | XP_013453882.1 | 94.8 | 460 | 24 | 0 | 1 | 460 | 1 | 460 | 3.10E-258 | 900.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59021.t1 | Q84WT5 | 38.1 | 467 | 256 | 6 | 7 | 460 | 10 | 456 | 1.8e-89 | 331.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59021.t1 | A0A072UFN4 | 94.8 | 460 | 24 | 0 | 1 | 460 | 1 | 460 | 2.3e-258 | 900.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene99993 | MS.gene59021 | PPI |
| MS.gene75410 | MS.gene59021 | PPI |
| MS.gene054660 | MS.gene59021 | PPI |
| MS.gene62099 | MS.gene59021 | PPI |
| MS.gene044959 | MS.gene59021 | PPI |
| MS.gene68749 | MS.gene59021 | PPI |
| MS.gene68149 | MS.gene59021 | PPI |
| MS.gene062453 | MS.gene59021 | PPI |
| MS.gene032541 | MS.gene59021 | PPI |
| MS.gene52090 | MS.gene59021 | PPI |
| MS.gene063101 | MS.gene59021 | PPI |
| MS.gene74061 | MS.gene59021 | PPI |
| MS.gene057723 | MS.gene59021 | PPI |
| MS.gene003507 | MS.gene59021 | PPI |
| MS.gene53185 | MS.gene59021 | PPI |
| MS.gene014213 | MS.gene59021 | PPI |
| MS.gene90963 | MS.gene59021 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59021.t1 | MTR_5g049180 | 94.783 | 460 | 24 | 0 | 1 | 460 | 1 | 460 | 0.0 | 919 |
| MS.gene59021.t1 | MTR_7g024420 | 83.444 | 453 | 75 | 0 | 1 | 453 | 1 | 453 | 0.0 | 783 |
| MS.gene59021.t1 | MTR_4g074070 | 73.348 | 454 | 121 | 0 | 1 | 454 | 1 | 454 | 0.0 | 714 |
| MS.gene59021.t1 | MTR_1g106200 | 47.921 | 457 | 233 | 3 | 9 | 460 | 18 | 474 | 1.59e-157 | 459 |
| MS.gene59021.t1 | MTR_1g106910 | 47.527 | 465 | 237 | 4 | 3 | 460 | 9 | 473 | 1.73e-153 | 449 |
| MS.gene59021.t1 | MTR_1g106790 | 46.725 | 458 | 238 | 4 | 8 | 460 | 17 | 473 | 2.88e-152 | 446 |
| MS.gene59021.t1 | MTR_1g106770 | 47.248 | 436 | 226 | 3 | 29 | 460 | 4 | 439 | 5.70e-148 | 433 |
| MS.gene59021.t1 | MTR_1g106200 | 42.451 | 457 | 225 | 4 | 9 | 460 | 24 | 447 | 6.50e-131 | 390 |
| MS.gene59021.t1 | MTR_3g117800 | 37.613 | 444 | 246 | 9 | 9 | 448 | 1 | 417 | 4.12e-93 | 292 |
| MS.gene59021.t1 | MTR_3g117800 | 41.525 | 354 | 200 | 5 | 99 | 448 | 16 | 366 | 6.47e-89 | 280 |
| MS.gene59021.t1 | MTR_3g117800 | 42.249 | 329 | 185 | 3 | 122 | 448 | 11 | 336 | 7.48e-86 | 271 |
| MS.gene59021.t1 | MTR_1g017020 | 31.279 | 438 | 269 | 10 | 43 | 454 | 361 | 792 | 4.36e-61 | 214 |
| MS.gene59021.t1 | MTR_3g103610 | 30.769 | 442 | 266 | 12 | 43 | 455 | 200 | 630 | 1.18e-59 | 208 |
| MS.gene59021.t1 | MTR_4g121520 | 41.892 | 148 | 60 | 1 | 305 | 452 | 223 | 344 | 4.98e-26 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59021.t1 | AT4G33840 | 56.164 | 438 | 188 | 2 | 16 | 451 | 19 | 454 | 0.0 | 535 |
| MS.gene59021.t1 | AT4G33830 | 55.142 | 457 | 200 | 3 | 7 | 460 | 11 | 465 | 0.0 | 531 |
| MS.gene59021.t1 | AT4G33860 | 55.708 | 438 | 190 | 2 | 16 | 451 | 19 | 454 | 0.0 | 520 |
| MS.gene59021.t1 | AT4G33810 | 38.116 | 467 | 262 | 5 | 1 | 460 | 10 | 456 | 1.69e-111 | 341 |
| MS.gene59021.t1 | AT4G33810 | 38.116 | 467 | 262 | 5 | 1 | 460 | 10 | 456 | 1.69e-111 | 341 |
| MS.gene59021.t1 | AT4G33810 | 38.116 | 467 | 262 | 5 | 1 | 460 | 10 | 456 | 1.69e-111 | 341 |
| MS.gene59021.t1 | AT4G33820 | 38.380 | 469 | 256 | 7 | 5 | 460 | 8 | 456 | 1.51e-110 | 338 |
| MS.gene59021.t1 | AT4G33810 | 43.008 | 379 | 211 | 3 | 87 | 460 | 38 | 416 | 1.91e-108 | 332 |
| MS.gene59021.t1 | AT4G33810 | 43.008 | 379 | 211 | 3 | 87 | 460 | 38 | 416 | 1.91e-108 | 332 |
| MS.gene59021.t1 | AT2G14690 | 36.971 | 449 | 250 | 6 | 18 | 460 | 28 | 449 | 1.99e-98 | 307 |
| MS.gene59021.t1 | AT4G38650 | 37.300 | 437 | 238 | 9 | 22 | 448 | 34 | 444 | 8.23e-96 | 300 |
| MS.gene59021.t1 | AT4G38300 | 44.747 | 257 | 130 | 2 | 192 | 448 | 12 | 256 | 9.83e-75 | 236 |
| MS.gene59021.t1 | AT1G58370 | 30.512 | 449 | 277 | 12 | 35 | 455 | 362 | 803 | 1.42e-62 | 219 |
| MS.gene59021.t1 | AT1G58370 | 30.512 | 449 | 277 | 12 | 35 | 455 | 390 | 831 | 1.46e-62 | 219 |
| MS.gene59021.t1 | AT1G10050 | 29.186 | 442 | 276 | 11 | 43 | 454 | 550 | 984 | 5.47e-56 | 201 |
| MS.gene59021.t1 | AT1G10050 | 29.186 | 442 | 276 | 11 | 43 | 454 | 518 | 952 | 8.35e-56 | 201 |
| MS.gene59021.t1 | AT1G10050 | 29.186 | 442 | 276 | 11 | 43 | 454 | 518 | 952 | 8.35e-56 | 201 |
| MS.gene59021.t1 | AT4G08160 | 28.665 | 457 | 283 | 11 | 35 | 455 | 190 | 639 | 2.61e-55 | 196 |
| MS.gene59021.t1 | AT4G08160 | 28.665 | 457 | 283 | 11 | 35 | 455 | 190 | 639 | 6.96e-55 | 196 |
Find 103 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTATCTTCTTGATATATTT+TGG | 0.098842 | 5.3:+37654553 | None:intergenic |
| ATAATACTTCTGTTGAAATT+TGG | 0.134584 | 5.3:-37655413 | MS.gene59021:CDS |
| TCAGAGGCCACAATATATTT+TGG | 0.162947 | 5.3:-37654861 | MS.gene59021:CDS |
| ACAACTGAAGGATTTAAATT+TGG | 0.187136 | 5.3:-37655657 | MS.gene59021:CDS |
| CTGCCACTGGTTTGCCAATA+TGG | 0.191945 | 5.3:-37654420 | MS.gene59021:CDS |
| GCAATAGTGCATATCATTAT+TGG | 0.208863 | 5.3:-37655017 | MS.gene59021:CDS |
| CAGAAAATTTACCTACAAAA+TGG | 0.235874 | 5.3:-37660556 | MS.gene59021:CDS |
| AATAGAACATAGAGAATCTT+TGG | 0.248709 | 5.3:-37660632 | MS.gene59021:CDS |
| GGATTGCATTATTCTTTATC+TGG | 0.257416 | 5.3:-37660535 | MS.gene59021:intron |
| ATGTGGTTGATTTCTGTTAT+TGG | 0.257701 | 5.3:+37660591 | None:intergenic |
| ACAATTCGGCTCAGCGTAAA+TGG | 0.263557 | 5.3:+37655629 | None:intergenic |
| CTATGTGTCATCCTACTTGC+AGG | 0.291154 | 5.3:-37661296 | MS.gene59021:intron |
| AATGAAGTTCACAACATTGA+TGG | 0.301444 | 5.3:-37654643 | MS.gene59021:CDS |
| AAGCAATTGCCTCTTGGAAT+TGG | 0.301823 | 5.3:-37654511 | MS.gene59021:CDS |
| TGTAGTCCATCATTCAGTTC+TGG | 0.305480 | 5.3:+37660682 | None:intergenic |
| ATCCTCAATACCAACCTTCT+TGG | 0.306095 | 5.3:-37654834 | MS.gene59021:CDS |
| TGAGAAATGATAAGCATAAT+TGG | 0.324011 | 5.3:+37654346 | None:intergenic |
| GTAATAGATATTCTTGCATT+TGG | 0.328996 | 5.3:+37655093 | None:intergenic |
| GAATTGTTGGTCTATGCTTA+AGG | 0.343869 | 5.3:-37655613 | MS.gene59021:CDS |
| GCTCAGGATGTATTTGTTCA+TGG | 0.357032 | 5.3:+37655038 | None:intergenic |
| ATCATTATTGGTTCGCATCT+AGG | 0.357864 | 5.3:-37655005 | MS.gene59021:CDS |
| CTACAAGGATGGACAGCATT+TGG | 0.360370 | 5.3:-37660664 | MS.gene59021:CDS |
| GCAATTGCTTATTCCAACTT+TGG | 0.363618 | 5.3:+37654524 | None:intergenic |
| CCATTGTACTGAGGCCTTTG+TGG | 0.366505 | 5.3:+37660721 | None:intergenic |
| CATTGTACTGAGGCCTTTGT+GGG | 0.387812 | 5.3:+37660722 | None:intergenic |
| GTTATCGACTTTACCTGATT+TGG | 0.388943 | 5.3:+37654370 | None:intergenic |
| TCTAATTCAGTGATCCATAT+TGG | 0.391697 | 5.3:+37654406 | None:intergenic |
| ATATTCTTGCATTTGGTAAA+GGG | 0.397992 | 5.3:+37655100 | None:intergenic |
| TGTTGCAAGCCAGCCAAATC+AGG | 0.400763 | 5.3:-37654383 | MS.gene59021:CDS |
| CTCGACACCCTTGCTGCCAC+TGG | 0.401901 | 5.3:-37654433 | MS.gene59021:CDS |
| AATGCTGGCACTATGATCAT+AGG | 0.417516 | 5.3:+37660846 | None:intergenic |
| TGGAATAAGCAATTGCCTCT+TGG | 0.419115 | 5.3:-37654517 | MS.gene59021:CDS |
| AGATACAAGGACCAGCTAAT+TGG | 0.419860 | 5.3:-37654745 | MS.gene59021:CDS |
| GATATTCTTGCATTTGGTAA+AGG | 0.422820 | 5.3:+37655099 | None:intergenic |
| TTTCGACTGCATTGTTAAGT+TGG | 0.426420 | 5.3:+37654791 | None:intergenic |
| AATTGTTGGTCTATGCTTAA+GGG | 0.427116 | 5.3:-37655612 | MS.gene59021:CDS |
| TACAAGGATGGACAGCATTT+GGG | 0.431640 | 5.3:-37660663 | MS.gene59021:CDS |
| GGTCTCACAGCGGACGCAAC+AGG | 0.436625 | 5.3:-37655588 | MS.gene59021:CDS |
| TTTACGCTGAGCCGAATTGT+TGG | 0.438915 | 5.3:-37655626 | MS.gene59021:CDS |
| ACTGAAGGATTTAAATTTGG+TGG | 0.441063 | 5.3:-37655654 | MS.gene59021:CDS |
| TCCTCAATACCAACCTTCTT+GGG | 0.445458 | 5.3:-37654833 | MS.gene59021:CDS |
| GCTCTCATGTAAGGCAAGTT+GGG | 0.446579 | 5.3:+37654460 | None:intergenic |
| GGCTCTCATGTAAGGCAAGT+TGG | 0.452068 | 5.3:+37654459 | None:intergenic |
| TCATTCTTTGAAAGTAAACT+AGG | 0.473187 | 5.3:-37654691 | MS.gene59021:CDS |
| ATAGAACATAGAGAATCTTT+GGG | 0.481544 | 5.3:-37660631 | MS.gene59021:CDS |
| ACCCAAGAAGGTTGGTATTG+AGG | 0.486924 | 5.3:+37654832 | None:intergenic |
| TTGGTTCGCATCTAGGTTCA+CGG | 0.486946 | 5.3:-37654998 | MS.gene59021:CDS |
| TAGTTTACTTTCAAAGAATG+AGG | 0.487897 | 5.3:+37654693 | None:intergenic |
| TTGTTCATGGCACTTCCAAA+TGG | 0.489868 | 5.3:+37655051 | None:intergenic |
| TCGAGGGAGGCTCTCATGTA+AGG | 0.492315 | 5.3:+37654451 | None:intergenic |
| CAAGGACCAGCTAATTGGTT+GGG | 0.496790 | 5.3:-37654740 | MS.gene59021:CDS |
| TCGACTTTACCTGATTTGGC+TGG | 0.510163 | 5.3:+37654374 | None:intergenic |
| TTAACAATGCAGTCGAAAAG+AGG | 0.510402 | 5.3:-37654786 | MS.gene59021:CDS |
| AATGATATGCACTATTGCTC+AGG | 0.510678 | 5.3:+37655022 | None:intergenic |
| TCTATTACAATGAAGAGGTA+TGG | 0.515108 | 5.3:-37655078 | MS.gene59021:CDS |
| TCAAGAACAGAATATTGAAA+AGG | 0.517817 | 5.3:-37655340 | MS.gene59021:intron |
| CAACATCCCAACCAATTAGC+TGG | 0.523794 | 5.3:+37654734 | None:intergenic |
| TTAAGCATAGACCAACAATT+CGG | 0.529114 | 5.3:+37655615 | None:intergenic |
| TTCTGTTGTGTCAAGATACA+AGG | 0.535595 | 5.3:-37654758 | MS.gene59021:CDS |
| GTACTACTACGTTCTTCTTG+CGG | 0.542899 | 5.3:+37655145 | None:intergenic |
| GAGAGAGCTTACTTCAATGC+TGG | 0.548545 | 5.3:+37660831 | None:intergenic |
| GGCAGCAAGGGTGTCGAGGG+AGG | 0.552078 | 5.3:+37654438 | None:intergenic |
| AGATAAGAGAGATCCAAAGT+TGG | 0.552172 | 5.3:-37654537 | MS.gene59021:CDS |
| TGGCAAACCAGTGGCAGCAA+GGG | 0.554004 | 5.3:+37654426 | None:intergenic |
| GAATGATTATAATACTATTG+AGG | 0.555610 | 5.3:-37654602 | MS.gene59021:CDS |
| GAGAGCGAAGGTACCCAAGA+AGG | 0.556728 | 5.3:+37654820 | None:intergenic |
| ACTATTGAGGATAGTAGAGA+TGG | 0.560205 | 5.3:-37654589 | MS.gene59021:CDS |
| CAGTGGCAGCAAGGGTGTCG+AGG | 0.563868 | 5.3:+37654434 | None:intergenic |
| AGTGGCAGCAAGGGTGTCGA+GGG | 0.570712 | 5.3:+37654435 | None:intergenic |
| ACAAGGACCAGCTAATTGGT+TGG | 0.574029 | 5.3:-37654741 | MS.gene59021:CDS |
| TTGGCAAACCAGTGGCAGCA+AGG | 0.582193 | 5.3:+37654425 | None:intergenic |
| TGTTGGTCTATGCTTAAGGG+TGG | 0.586142 | 5.3:-37655609 | MS.gene59021:CDS |
| AAATGTCCCGGTGACAGCAA+TGG | 0.591314 | 5.3:-37655685 | MS.gene59021:CDS |
| TGAATGATGGACTACAAGGA+TGG | 0.593461 | 5.3:-37660675 | MS.gene59021:CDS |
| AGTGAGTGAGGCAAATGTCC+CGG | 0.594400 | 5.3:-37655697 | MS.gene59021:CDS |
| GAACTGAATGATGGACTACA+AGG | 0.594598 | 5.3:-37660679 | MS.gene59021:CDS |
| GAATATCTATTACAATGAAG+AGG | 0.596129 | 5.3:-37655083 | MS.gene59021:CDS |
| CATTTGAAAATGAAATGAAG+TGG | 0.596860 | 5.3:-37654969 | MS.gene59021:CDS |
| GTACAAGCACTTGCTGAACA+AGG | 0.600805 | 5.3:-37655126 | MS.gene59021:CDS |
| GCAATGGTCAAAACAACTGA+AGG | 0.603923 | 5.3:-37655669 | MS.gene59021:CDS |
| CCACAAAGGCCTCAGTACAA+TGG | 0.608931 | 5.3:-37660721 | MS.gene59021:CDS |
| ATCTTTGGGTAACAAATATG+TGG | 0.609080 | 5.3:-37660617 | MS.gene59021:CDS |
| GCGAAGGTACCCAAGAAGGT+TGG | 0.609945 | 5.3:+37654824 | None:intergenic |
| GAATGAATATGCACAAGGCA+AGG | 0.611352 | 5.3:-37654941 | MS.gene59021:CDS |
| GATCCTCCCAAAATATATTG+TGG | 0.612097 | 5.3:+37654854 | None:intergenic |
| ATGATTCCTCCATTGTACTG+AGG | 0.614806 | 5.3:+37660712 | None:intergenic |
| AGTTGGTCTGCTGAGAGCGA+AGG | 0.615345 | 5.3:+37654808 | None:intergenic |
| TTGAAGCCGATGTGATGCTA+CGG | 0.615957 | 5.3:-37654909 | MS.gene59021:CDS |
| ATAATCATTCATGAACAAAG+TGG | 0.625091 | 5.3:+37654615 | None:intergenic |
| GTAGGATGACACATAGCAGA+AGG | 0.636550 | 5.3:+37661303 | None:intergenic |
| CTCATGTAAGGCAAGTTGGG+AGG | 0.637521 | 5.3:+37654463 | None:intergenic |
| TGCATACCGTAGCATCACAT+CGG | 0.645236 | 5.3:+37654903 | None:intergenic |
| GTGGTTACAAACCTGCAAGT+AGG | 0.646101 | 5.3:+37661285 | None:intergenic |
| AGTGTTTGGCACACCCACAA+AGG | 0.649093 | 5.3:-37660735 | MS.gene59021:CDS |
| CAAAGGCCTCAGTACAATGG+AGG | 0.654580 | 5.3:-37660718 | MS.gene59021:CDS |
| AGCTTGGATACAAGTGAGTG+AGG | 0.658683 | 5.3:-37655709 | MS.gene59021:CDS |
| GATCCATATTGGCAAACCAG+TGG | 0.665618 | 5.3:+37654417 | None:intergenic |
| AAACACGGTATTGCTGTCAG+AGG | 0.669925 | 5.3:-37654877 | MS.gene59021:CDS |
| CTACGGTATGCAAAGAAACA+CGG | 0.679684 | 5.3:-37654892 | MS.gene59021:CDS |
| GACTCAAGTCCAATTCCAAG+AGG | 0.679929 | 5.3:+37654502 | None:intergenic |
| TTCTGTGAGACACTATCATG+TGG | 0.686906 | 5.3:+37660574 | None:intergenic |
| TACACGAATGAATATGCACA+AGG | 0.712550 | 5.3:-37654946 | MS.gene59021:CDS |
| GCTTAAGGGTGGTCTCACAG+CGG | 0.764795 | 5.3:-37655598 | MS.gene59021:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 37654318 | 37661336 | 37654318 | ID=MS.gene59021 |
| chr5.3 | mRNA | 37654318 | 37661336 | 37654318 | ID=MS.gene59021.t1;Parent=MS.gene59021 |
| chr5.3 | exon | 37661297 | 37661336 | 37661297 | ID=MS.gene59021.t1.exon1;Parent=MS.gene59021.t1 |
| chr5.3 | CDS | 37661297 | 37661336 | 37661297 | ID=cds.MS.gene59021.t1;Parent=MS.gene59021.t1 |
| chr5.3 | exon | 37660843 | 37660889 | 37660843 | ID=MS.gene59021.t1.exon2;Parent=MS.gene59021.t1 |
| chr5.3 | CDS | 37660843 | 37660889 | 37660843 | ID=cds.MS.gene59021.t1;Parent=MS.gene59021.t1 |
| chr5.3 | exon | 37660536 | 37660755 | 37660536 | ID=MS.gene59021.t1.exon3;Parent=MS.gene59021.t1 |
| chr5.3 | CDS | 37660536 | 37660755 | 37660536 | ID=cds.MS.gene59021.t1;Parent=MS.gene59021.t1 |
| chr5.3 | exon | 37655566 | 37655729 | 37655566 | ID=MS.gene59021.t1.exon4;Parent=MS.gene59021.t1 |
| chr5.3 | CDS | 37655566 | 37655729 | 37655566 | ID=cds.MS.gene59021.t1;Parent=MS.gene59021.t1 |
| chr5.3 | exon | 37655341 | 37655439 | 37655341 | ID=MS.gene59021.t1.exon5;Parent=MS.gene59021.t1 |
| chr5.3 | CDS | 37655341 | 37655439 | 37655341 | ID=cds.MS.gene59021.t1;Parent=MS.gene59021.t1 |
| chr5.3 | exon | 37654318 | 37655169 | 37654318 | ID=MS.gene59021.t1.exon6;Parent=MS.gene59021.t1 |
| chr5.3 | CDS | 37654318 | 37655169 | 37654318 | ID=cds.MS.gene59021.t1;Parent=MS.gene59021.t1 |
| Gene Sequence |
| Protein sequence |
