Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59901.t1 | XP_013445298.1 | 96.6 | 178 | 6 | 0 | 1 | 178 | 206 | 383 | 1.70E-95 | 358.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59901.t1 | Q7VG78 | 42.9 | 168 | 94 | 1 | 2 | 167 | 801 | 968 | 1.8e-37 | 157.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59901.t1 | A0A072TP99 | 96.6 | 178 | 6 | 0 | 1 | 178 | 206 | 383 | 1.2e-95 | 358.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049501 | MS.gene59901 | 0.828299 | 9.71E-55 | -1.69E-46 |
| MS.gene050226 | MS.gene59901 | 0.802775 | 4.78E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene59901 | 0.820064 | 8.36E-53 | -1.69E-46 |
| MS.gene050228 | MS.gene59901 | 0.817689 | 2.90E-52 | -1.69E-46 |
| MS.gene050438 | MS.gene59901 | 0.813573 | 2.39E-51 | -1.69E-46 |
| MS.gene050442 | MS.gene59901 | 0.843241 | 1.58E-58 | -1.69E-46 |
| MS.gene050586 | MS.gene59901 | 0.821533 | 3.84E-53 | -1.69E-46 |
| MS.gene050719 | MS.gene59901 | 0.844064 | 9.53E-59 | -1.69E-46 |
| MS.gene050721 | MS.gene59901 | 0.842402 | 2.64E-58 | -1.69E-46 |
| MS.gene050724 | MS.gene59901 | 0.81598 | 7.00E-52 | -1.69E-46 |
| MS.gene050780 | MS.gene59901 | 0.811563 | 6.58E-51 | -1.69E-46 |
| MS.gene050787 | MS.gene59901 | 0.818847 | 1.58E-52 | -1.69E-46 |
| MS.gene050807 | MS.gene59901 | 0.809888 | 1.51E-50 | -1.69E-46 |
| MS.gene050959 | MS.gene59901 | 0.828524 | 8.57E-55 | -1.69E-46 |
| MS.gene052323 | MS.gene59901 | 0.825819 | 3.81E-54 | -1.69E-46 |
| MS.gene052561 | MS.gene59901 | 0.820431 | 6.88E-53 | -1.69E-46 |
| MS.gene052564 | MS.gene59901 | 0.80597 | 1.03E-49 | -1.69E-46 |
| MS.gene052565 | MS.gene59901 | 0.805013 | 1.64E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene59901 | 0.844548 | 7.06E-59 | -1.69E-46 |
| MS.gene052896 | MS.gene59901 | 0.804567 | 2.03E-49 | -1.69E-46 |
| MS.gene053630 | MS.gene59901 | 0.80902 | 2.33E-50 | -1.69E-46 |
| MS.gene054468 | MS.gene59901 | 0.810861 | 9.34E-51 | -1.69E-46 |
| MS.gene054473 | MS.gene59901 | 0.810867 | 9.31E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene59901 | 0.809562 | 1.78E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene59901 | 0.834299 | 3.25E-56 | -1.69E-46 |
| MS.gene055468 | MS.gene59901 | 0.828168 | 1.04E-54 | -1.69E-46 |
| MS.gene055778 | MS.gene59901 | 0.814987 | 1.16E-51 | -1.69E-46 |
| MS.gene056069 | MS.gene59901 | -0.808742 | 2.67E-50 | -1.69E-46 |
| MS.gene057869 | MS.gene59901 | 0.805026 | 1.63E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene59901 | 0.829872 | 4.04E-55 | -1.69E-46 |
| MS.gene058636 | MS.gene59901 | 0.835276 | 1.84E-56 | -1.69E-46 |
| MS.gene060404 | MS.gene59901 | 0.830719 | 2.51E-55 | -1.69E-46 |
| MS.gene060572 | MS.gene59901 | 0.80166 | 8.11E-49 | -1.69E-46 |
| MS.gene060690 | MS.gene59901 | 0.823192 | 1.58E-53 | -1.69E-46 |
| MS.gene061040 | MS.gene59901 | -0.80244 | 5.61E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGAAACGTCAGAATTTC+AGG | 0.191617 | 8.3:+53089613 | MS.gene59901:CDS |
| TTGTCTATTAGTTTGGAATA+TGG | 0.244808 | 8.3:+53089977 | MS.gene59901:CDS |
| ACAAATGTTGTCTATTAGTT+TGG | 0.247258 | 8.3:+53089970 | MS.gene59901:CDS |
| ATGTCTTCACTGGAATCTTA+TGG | 0.298466 | 8.3:-53090806 | None:intergenic |
| AACTTGTATTGTGTGGAAAT+AGG | 0.315120 | 8.3:-53090780 | None:intergenic |
| ACATGGTCAGAAGAGGCTTT+AGG | 0.333417 | 8.3:+53090853 | MS.gene59901:CDS |
| ATTTCCACACAATACAAGTT+TGG | 0.362445 | 8.3:+53090783 | MS.gene59901:CDS |
| GTTCATTTGATAAATACATA+TGG | 0.363388 | 8.3:+53090739 | MS.gene59901:CDS |
| AGAATTTAATGCAGCAACTT+TGG | 0.368329 | 8.3:+53089931 | MS.gene59901:CDS |
| TTCTCATCAGCATGACAATA+AGG | 0.369609 | 8.3:-53090967 | None:intergenic |
| AGAAGAGGCTTTAGGTTTGT+AGG | 0.376445 | 8.3:+53090861 | MS.gene59901:CDS |
| ATCATGATAAGCAATATAGA+TGG | 0.377489 | 8.3:-53089385 | None:intergenic |
| TGCTTATCATGATGAGGAAT+GGG | 0.384161 | 8.3:+53089395 | MS.gene59901:CDS |
| CTGACGTTTCTTCAAGATTG+AGG | 0.413643 | 8.3:-53089604 | None:intergenic |
| TTCATTTGATAAATACATAT+GGG | 0.421482 | 8.3:+53090740 | MS.gene59901:CDS |
| CATTTGTTTATCAGTTAAGC+TGG | 0.466390 | 8.3:-53089954 | None:intergenic |
| TTACAGGTTAACAAGGACTT+TGG | 0.477182 | 8.3:+53090717 | MS.gene59901:intron |
| ACTTACATGTCATCATGAAC+TGG | 0.482944 | 8.3:-53089423 | None:intergenic |
| CATTCGTTTATGCAAGCAGC+TGG | 0.484640 | 8.3:+53090897 | MS.gene59901:CDS |
| TTGCTTATCATGATGAGGAA+TGG | 0.508626 | 8.3:+53089394 | MS.gene59901:CDS |
| GAAGAGGCTTTAGGTTTGTA+GGG | 0.509442 | 8.3:+53090862 | MS.gene59901:CDS |
| GTGCTCAAGTTGGATCTGAT+TGG | 0.533668 | 8.3:+53089580 | MS.gene59901:CDS |
| ATCGACATAAGCAGAGTTCG+AGG | 0.564739 | 8.3:+53090001 | MS.gene59901:CDS |
| AGGTGCATTTCAAGTGGCTG+TGG | 0.567532 | 8.3:-53090947 | None:intergenic |
| GCAAGTGATTAAGTGGTCAT+TGG | 0.573542 | 8.3:-53090925 | None:intergenic |
| GGCTGTGGCAAGTGATTAAG+TGG | 0.581468 | 8.3:-53090932 | None:intergenic |
| TCATTTGATAAATACATATG+GGG | 0.581577 | 8.3:+53090741 | MS.gene59901:CDS |
| TCATGATAAGCAATATAGAT+GGG | 0.591991 | 8.3:-53089384 | None:intergenic |
| CTATATTGCTTATCATGATG+AGG | 0.598338 | 8.3:+53089389 | MS.gene59901:CDS |
| AGCAAAGACATGGTCAGAAG+AGG | 0.606430 | 8.3:+53090846 | MS.gene59901:CDS |
| TGATTAAGTGGTCATTGGTG+AGG | 0.631169 | 8.3:-53090920 | None:intergenic |
| ATGGCCAAACTTGTATTGTG+TGG | 0.631469 | 8.3:-53090787 | None:intergenic |
| GTTATATTTACAGGTTAACA+AGG | 0.634646 | 8.3:+53090710 | MS.gene59901:intron |
| ACAATAAGGTGCATTTCAAG+TGG | 0.639984 | 8.3:-53090953 | None:intergenic |
| ATAAACGAATGAATCACTGT+TGG | 0.649684 | 8.3:-53090885 | None:intergenic |
| GCTTATCATGATGAGGAATG+GGG | 0.660568 | 8.3:+53089396 | MS.gene59901:CDS |
| AGAGAGCATAAGCAAAGACA+TGG | 0.662828 | 8.3:+53090836 | MS.gene59901:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATATTCTTTCTGTAAAT+TGG | + | chr8.3:53090684-53090703 | MS.gene59901:intron | 15.0% |
| !! | TTCATTTGATAAATACATAT+GGG | + | chr8.3:53090740-53090759 | MS.gene59901:CDS | 15.0% |
| !!! | TGAAATTTAAATTCTTTTAC+AGG | + | chr8.3:53089897-53089916 | MS.gene59901:intron | 15.0% |
| !! | AATTAAAATCTCAATCTTGT+CGG | - | chr8.3:53090387-53090406 | None:intergenic | 20.0% |
| !! | GTTCATTTGATAAATACATA+TGG | + | chr8.3:53090739-53090758 | MS.gene59901:CDS | 20.0% |
| !! | TCATTTGATAAATACATATG+GGG | + | chr8.3:53090741-53090760 | MS.gene59901:CDS | 20.0% |
| !! | TTGTTAACCTGTAAATATAA+CGG | - | chr8.3:53090711-53090730 | None:intergenic | 20.0% |
| ! | AAACAAATATGAAATGTACC+TGG | + | chr8.3:53090608-53090627 | MS.gene59901:intron | 25.0% |
| ! | ACAAATGTTGTCTATTAGTT+TGG | + | chr8.3:53089970-53089989 | MS.gene59901:CDS | 25.0% |
| ! | AGAGATCAAACAGAATATAT+GGG | - | chr8.3:53090416-53090435 | None:intergenic | 25.0% |
| ! | ATCATGATAAGCAATATAGA+TGG | - | chr8.3:53089388-53089407 | None:intergenic | 25.0% |
| ! | ATTAAAATCTCAATCTTGTC+GGG | - | chr8.3:53090386-53090405 | None:intergenic | 25.0% |
| ! | GAGAATATAGTTCAAAATTG+CGG | - | chr8.3:53090207-53090226 | None:intergenic | 25.0% |
| ! | GCATATTATGATATTATAGC+TGG | + | chr8.3:53090109-53090128 | MS.gene59901:intron | 25.0% |
| ! | GTTATATTTACAGGTTAACA+AGG | + | chr8.3:53090710-53090729 | MS.gene59901:intron | 25.0% |
| ! | TAGAGATCAAACAGAATATA+TGG | - | chr8.3:53090417-53090436 | None:intergenic | 25.0% |
| ! | TCATGATAAGCAATATAGAT+GGG | - | chr8.3:53089387-53089406 | None:intergenic | 25.0% |
| !! | TAATGCTAATCGAATTTTAG+AGG | + | chr8.3:53090033-53090052 | MS.gene59901:CDS | 25.0% |
| !! | TCTTTGTTATAGTTGAAGTA+AGG | - | chr8.3:53090334-53090353 | None:intergenic | 25.0% |
| !! | TTGTCTATTAGTTTGGAATA+TGG | + | chr8.3:53089977-53089996 | MS.gene59901:CDS | 25.0% |
| !!! | ATGTACTTTTTTCAGAATGA+GGG | + | chr8.3:53089868-53089887 | MS.gene59901:intron | 25.0% |
| !!! | TATGTACTTTTTTCAGAATG+AGG | + | chr8.3:53089867-53089886 | MS.gene59901:intron | 25.0% |
| !!! | TTTGAACTTCTAGTTTTAAG+TGG | + | chr8.3:53089558-53089577 | MS.gene59901:CDS | 25.0% |
| AACTTGTATTGTGTGGAAAT+AGG | - | chr8.3:53090783-53090802 | None:intergenic | 30.0% | |
| AATTGGTCCGTTATATTTAC+AGG | + | chr8.3:53090701-53090720 | MS.gene59901:intron | 30.0% | |
| AGAATTTAATGCAGCAACTT+TGG | + | chr8.3:53089931-53089950 | MS.gene59901:CDS | 30.0% | |
| ATAAACGAATGAATCACTGT+TGG | - | chr8.3:53090888-53090907 | None:intergenic | 30.0% | |
| ATTTCCACACAATACAAGTT+TGG | + | chr8.3:53090783-53090802 | MS.gene59901:CDS | 30.0% | |
| CATTTGTTTATCAGTTAAGC+TGG | - | chr8.3:53089957-53089976 | None:intergenic | 30.0% | |
| CTATATTGCTTATCATGATG+AGG | + | chr8.3:53089389-53089408 | MS.gene59901:CDS | 30.0% | |
| GAGATCAAACAGAATATATG+GGG | - | chr8.3:53090415-53090434 | None:intergenic | 30.0% | |
| GTAGAATGTTCACTAAAAAC+AGG | - | chr8.3:53090243-53090262 | None:intergenic | 30.0% | |
| ! | ATAAGTGAGATCGAGATTTT+AGG | - | chr8.3:53090458-53090477 | None:intergenic | 30.0% |
| !! | TCATTGTGTTTTTGTGTGAT+GGG | - | chr8.3:53089463-53089482 | None:intergenic | 30.0% |
| !! | TTATAGCTGGTTCTAAATTG+TGG | + | chr8.3:53090122-53090141 | MS.gene59901:intron | 30.0% |
| !! | TTCATTGTGTTTTTGTGTGA+TGG | - | chr8.3:53089464-53089483 | None:intergenic | 30.0% |
| !!! | TGTACTTTTTTCAGAATGAG+GGG | + | chr8.3:53089869-53089888 | MS.gene59901:intron | 30.0% |
| AAAAAAGAGAGACCAAGTAG+AGG | - | chr8.3:53090089-53090108 | None:intergenic | 35.0% | |
| ACAATAAGGTGCATTTCAAG+TGG | - | chr8.3:53090956-53090975 | None:intergenic | 35.0% | |
| ACTTACATGTCATCATGAAC+TGG | - | chr8.3:53089426-53089445 | None:intergenic | 35.0% | |
| ATGAAATGTACCTGGAGTAT+TGG | + | chr8.3:53090616-53090635 | MS.gene59901:intron | 35.0% | |
| ATGTCTTCACTGGAATCTTA+TGG | - | chr8.3:53090809-53090828 | None:intergenic | 35.0% | |
| GTTTGACACTAACACTAACA+CGG | + | chr8.3:53089700-53089719 | MS.gene59901:intron | 35.0% | |
| TGAAGAAACGTCAGAATTTC+AGG | + | chr8.3:53089613-53089632 | MS.gene59901:CDS | 35.0% | |
| TGCTTATCATGATGAGGAAT+GGG | + | chr8.3:53089395-53089414 | MS.gene59901:CDS | 35.0% | |
| TTACAGGTTAACAAGGACTT+TGG | + | chr8.3:53090717-53090736 | MS.gene59901:intron | 35.0% | |
| TTGCTTATCATGATGAGGAA+TGG | + | chr8.3:53089394-53089413 | MS.gene59901:CDS | 35.0% | |
| ! | AAAAATGCTTTTGCACATGC+AGG | + | chr8.3:53089529-53089548 | MS.gene59901:intron | 35.0% |
| ! | TGAAATGTACCTGGAGTATT+GGG | + | chr8.3:53090617-53090636 | MS.gene59901:intron | 35.0% |
| !! | TCTGATTTTGATGTCTTCAC+TGG | - | chr8.3:53090819-53090838 | None:intergenic | 35.0% |
| AGACCAAGTAGAGGAACAAA+AGG | - | chr8.3:53090080-53090099 | None:intergenic | 40.0% | |
| ATGGCCAAACTTGTATTGTG+TGG | - | chr8.3:53090790-53090809 | None:intergenic | 40.0% | |
| ATGTGACACATGTCACATTG+TGG | + | chr8.3:53090519-53090538 | MS.gene59901:intron | 40.0% | |
| ATGTGACATGTGTCACATTG+TGG | - | chr8.3:53090517-53090536 | None:intergenic | 40.0% | |
| ATTCCACTTGTGTGACATTG+TGG | - | chr8.3:53090571-53090590 | None:intergenic | 40.0% | |
| CAAACAGAATATATGGGGTC+AGG | - | chr8.3:53090410-53090429 | None:intergenic | 40.0% | |
| CTGACGTTTCTTCAAGATTG+AGG | - | chr8.3:53089607-53089626 | None:intergenic | 40.0% | |
| GATCTCACTTATGTGACTTG+TGG | + | chr8.3:53090466-53090485 | MS.gene59901:intron | 40.0% | |
| GCAAGTGATTAAGTGGTCAT+TGG | - | chr8.3:53090928-53090947 | None:intergenic | 40.0% | |
| GCTTATCATGATGAGGAATG+GGG | + | chr8.3:53089396-53089415 | MS.gene59901:CDS | 40.0% | |
| TGATTAAGTGGTCATTGGTG+AGG | - | chr8.3:53090923-53090942 | None:intergenic | 40.0% | |
| ! | AGAGAGCATAAGCAAAGACA+TGG | + | chr8.3:53090836-53090855 | MS.gene59901:CDS | 40.0% |
| ! | GAAACGTCAGAATTTCAGGT+TGG | + | chr8.3:53089617-53089636 | MS.gene59901:intron | 40.0% |
| ! | GACAGTGTAGGTATTGTGTT+TGG | + | chr8.3:53089790-53089809 | MS.gene59901:intron | 40.0% |
| !! | AGAAGAGGCTTTAGGTTTGT+AGG | + | chr8.3:53090861-53090880 | MS.gene59901:CDS | 40.0% |
| !! | CACTAACACGGTATTGATAC+TGG | + | chr8.3:53089712-53089731 | MS.gene59901:intron | 40.0% |
| !! | CTGGTTCTAAATTGTGGTTG+CGG | + | chr8.3:53090128-53090147 | MS.gene59901:intron | 40.0% |
| !! | GAAATGTACCTGGAGTATTG+GGG | + | chr8.3:53090618-53090637 | MS.gene59901:intron | 40.0% |
| !! | GAAGAGGCTTTAGGTTTGTA+GGG | + | chr8.3:53090862-53090881 | MS.gene59901:CDS | 40.0% |
| !! | GTTTTAAGTGGTGCTCAAGT+TGG | + | chr8.3:53089570-53089589 | MS.gene59901:CDS | 40.0% |
| ACATGGTCAGAAGAGGCTTT+AGG | + | chr8.3:53090853-53090872 | MS.gene59901:CDS | 45.0% | |
| AGCAAAGACATGGTCAGAAG+AGG | + | chr8.3:53090846-53090865 | MS.gene59901:CDS | 45.0% | |
| ATCGACATAAGCAGAGTTCG+AGG | + | chr8.3:53090001-53090020 | MS.gene59901:CDS | 45.0% | |
| CATTCGTTTATGCAAGCAGC+TGG | + | chr8.3:53090897-53090916 | MS.gene59901:CDS | 45.0% | |
| TGACATGTGTCACATTGTGG+TGG | - | chr8.3:53090514-53090533 | None:intergenic | 45.0% | |
| ! | AGTAGAGGAACAAAAGGCAC+AGG | - | chr8.3:53090074-53090093 | None:intergenic | 45.0% |
| ! | GTGCCTTTTGTTCCTCTACT+TGG | + | chr8.3:53090074-53090093 | MS.gene59901:intron | 45.0% |
| !! | GTGCTCAAGTTGGATCTGAT+TGG | + | chr8.3:53089580-53089599 | MS.gene59901:CDS | 45.0% |
| CACTGTCACTGAGACAGACA+CGG | - | chr8.3:53089777-53089796 | None:intergenic | 50.0% | |
| CACTTGTGTGACATTGTGGC+GGG | - | chr8.3:53090567-53090586 | None:intergenic | 50.0% | |
| CCACTTGTGTGACATTGTGG+CGG | - | chr8.3:53090568-53090587 | None:intergenic | 50.0% | |
| GGCTGTGGCAAGTGATTAAG+TGG | - | chr8.3:53090935-53090954 | None:intergenic | 50.0% | |
| GTCTGTCTCAGTGACAGTGT+AGG | + | chr8.3:53089778-53089797 | MS.gene59901:intron | 50.0% | |
| ! | AGGTGCATTTCAAGTGGCTG+TGG | - | chr8.3:53090950-53090969 | None:intergenic | 50.0% |
| !! | AGAGAGTACCCCAATACTCC+AGG | - | chr8.3:53090629-53090648 | None:intergenic | 50.0% |
| !! | GTGTCACATTGTGGTGGACA+AGG | - | chr8.3:53090508-53090527 | None:intergenic | 50.0% |
| CCGCCACAATGTCACACAAG+TGG | + | chr8.3:53090565-53090584 | MS.gene59901:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 53089382 | 53090983 | 53089382 | ID=MS.gene59901 |
| chr8.3 | mRNA | 53089382 | 53090983 | 53089382 | ID=MS.gene59901.t1;Parent=MS.gene59901 |
| chr8.3 | exon | 53089382 | 53089439 | 53089382 | ID=MS.gene59901.t1.exon1;Parent=MS.gene59901.t1 |
| chr8.3 | CDS | 53089382 | 53089439 | 53089382 | ID=cds.MS.gene59901.t1;Parent=MS.gene59901.t1 |
| chr8.3 | exon | 53089551 | 53089634 | 53089551 | ID=MS.gene59901.t1.exon2;Parent=MS.gene59901.t1 |
| chr8.3 | CDS | 53089551 | 53089634 | 53089551 | ID=cds.MS.gene59901.t1;Parent=MS.gene59901.t1 |
| chr8.3 | exon | 53089919 | 53090054 | 53089919 | ID=MS.gene59901.t1.exon3;Parent=MS.gene59901.t1 |
| chr8.3 | CDS | 53089919 | 53090054 | 53089919 | ID=cds.MS.gene59901.t1;Parent=MS.gene59901.t1 |
| chr8.3 | exon | 53090723 | 53090983 | 53090723 | ID=MS.gene59901.t1.exon4;Parent=MS.gene59901.t1 |
| chr8.3 | CDS | 53090723 | 53090983 | 53090723 | ID=cds.MS.gene59901.t1;Parent=MS.gene59901.t1 |
| Gene Sequence |
| Protein sequence |
