Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052565.t1 | XP_003608254.1 | 98.3 | 577 | 10 | 0 | 1 | 577 | 1 | 577 | 0.00E+00 | 1094 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052565.t1 | G7JRW0 | 98.3 | 577 | 10 | 0 | 1 | 577 | 1 | 577 | 0.0e+00 | 1094.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052564 | MS.gene052565 | 0.998263 | 3.04E-260 | -1.69E-46 |
| MS.gene052565 | MS.gene066900 | 0.826356 | 2.84E-54 | -1.69E-46 |
| MS.gene052565 | MS.gene067234 | 0.81498 | 1.17E-51 | -1.69E-46 |
| MS.gene052565 | MS.gene30964 | 0.815488 | 9.01E-52 | -1.69E-46 |
| MS.gene052565 | MS.gene32213 | 0.988978 | 3.44E-176 | -1.69E-46 |
| MS.gene052565 | MS.gene33842 | 0.997961 | 6.21E-253 | -1.69E-46 |
| MS.gene052565 | MS.gene45498 | 0.818889 | 1.55E-52 | -1.69E-46 |
| MS.gene052565 | MS.gene48442 | -0.812079 | 5.08E-51 | -1.69E-46 |
| MS.gene052565 | MS.gene49330 | 0.808502 | 3.00E-50 | -1.69E-46 |
| MS.gene052565 | MS.gene50595 | 0.813322 | 2.71E-51 | -1.69E-46 |
| MS.gene052565 | MS.gene57920 | 0.812947 | 3.28E-51 | -1.69E-46 |
| MS.gene052565 | MS.gene59901 | 0.805013 | 1.64E-49 | -1.69E-46 |
| MS.gene052565 | MS.gene62431 | 0.809463 | 1.87E-50 | -1.69E-46 |
| MS.gene052565 | MS.gene70223 | -0.805248 | 1.46E-49 | -1.69E-46 |
| MS.gene052565 | MS.gene85443 | 0.801008 | 1.10E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene052565 | MS.gene023795 | PPI |
| MS.gene055531 | MS.gene052565 | PPI |
| MS.gene052565 | MS.gene57486 | PPI |
| MS.gene052565 | MS.gene52172 | PPI |
| MS.gene052565 | MS.gene06253 | PPI |
| MS.gene013180 | MS.gene052565 | PPI |
| MS.gene052565 | MS.gene019466 | PPI |
| MS.gene27647 | MS.gene052565 | PPI |
| MS.gene41659 | MS.gene052565 | PPI |
| MS.gene052565 | MS.gene36899 | PPI |
| MS.gene42235 | MS.gene052565 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052565.t1 | MTR_4g091320 | 98.267 | 577 | 10 | 0 | 1 | 577 | 1 | 577 | 0.0 | 1151 |
| MS.gene052565.t1 | MTR_4g007850 | 90.683 | 161 | 12 | 1 | 417 | 577 | 365 | 522 | 1.53e-92 | 294 |
| MS.gene052565.t1 | MTR_8g032570 | 31.625 | 566 | 336 | 18 | 27 | 572 | 48 | 582 | 1.75e-67 | 229 |
| MS.gene052565.t1 | MTR_8g032570 | 31.625 | 566 | 336 | 18 | 27 | 572 | 25 | 559 | 2.08e-67 | 229 |
| MS.gene052565.t1 | MTR_7g076220 | 30.526 | 570 | 345 | 15 | 22 | 570 | 17 | 556 | 3.04e-65 | 223 |
| MS.gene052565.t1 | MTR_3g098730 | 29.050 | 537 | 356 | 14 | 38 | 567 | 29 | 547 | 2.00e-45 | 169 |
| MS.gene052565.t1 | MTR_1g018180 | 23.318 | 223 | 165 | 3 | 201 | 423 | 103 | 319 | 7.40e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052565.t1 | AT1G61350 | 60.917 | 545 | 203 | 6 | 40 | 577 | 32 | 573 | 0.0 | 645 |
| MS.gene052565.t1 | AT2G45720 | 31.449 | 566 | 339 | 15 | 22 | 572 | 20 | 551 | 2.05e-69 | 234 |
| MS.gene052565.t1 | AT2G45720 | 31.449 | 566 | 339 | 15 | 22 | 572 | 20 | 551 | 2.05e-69 | 234 |
| MS.gene052565.t1 | AT2G45720 | 31.449 | 566 | 339 | 15 | 22 | 572 | 20 | 551 | 2.05e-69 | 234 |
| MS.gene052565.t1 | AT2G45720 | 31.449 | 566 | 339 | 15 | 22 | 572 | 20 | 551 | 2.05e-69 | 234 |
| MS.gene052565.t1 | AT2G45720 | 31.449 | 566 | 339 | 15 | 22 | 572 | 20 | 551 | 2.05e-69 | 234 |
| MS.gene052565.t1 | AT5G50900 | 31.588 | 554 | 354 | 12 | 23 | 567 | 11 | 548 | 1.97e-62 | 215 |
| MS.gene052565.t1 | AT1G01830 | 28.445 | 566 | 362 | 15 | 22 | 572 | 36 | 573 | 9.26e-59 | 206 |
| MS.gene052565.t1 | AT1G01830 | 28.445 | 566 | 362 | 15 | 22 | 572 | 36 | 573 | 9.26e-59 | 206 |
| MS.gene052565.t1 | AT1G01830 | 28.445 | 566 | 362 | 15 | 22 | 572 | 36 | 573 | 9.26e-59 | 206 |
| MS.gene052565.t1 | AT2G05810 | 29.853 | 546 | 358 | 16 | 38 | 571 | 40 | 572 | 1.24e-52 | 189 |
| MS.gene052565.t1 | AT2G05810 | 29.853 | 546 | 358 | 16 | 38 | 571 | 40 | 572 | 1.24e-52 | 189 |
| MS.gene052565.t1 | AT4G16490 | 24.451 | 319 | 217 | 8 | 143 | 447 | 157 | 465 | 7.80e-12 | 68.2 |
Find 131 sgRNAs with CRISPR-Local
Find 153 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACCTGAATTTCCCTCTTC+TGG | 0.157688 | 4.3:+22681112 | None:intergenic |
| CGCACCTTTGATCCGGGTTT+TGG | 0.178046 | 4.3:-22681902 | MS.gene052565:CDS |
| ACAAACCTCTTTCTATTTCT+AGG | 0.200258 | 4.3:+22681178 | None:intergenic |
| TGAGGAGGTTAAGAGGTTTA+TGG | 0.200986 | 4.3:-22681689 | MS.gene052565:CDS |
| TTGGATTGTGGGAGTGAGTT+GGG | 0.213740 | 4.3:-22681883 | MS.gene052565:CDS |
| AAAGTGGCATTTAGATTATC+TGG | 0.248155 | 4.3:-22681331 | MS.gene052565:CDS |
| ATTTGGTTGGTCCTGCTTGT+GGG | 0.301326 | 4.3:-22681735 | MS.gene052565:CDS |
| TCAGGGTCTTCGCCGGAAAA+TGG | 0.303092 | 4.3:-22682512 | MS.gene052565:CDS |
| TTGCCGACTCCGGCGAGCTT+TGG | 0.323964 | 4.3:-22682571 | MS.gene052565:CDS |
| TGAAGTTTCGATTCAAGAAT+TGG | 0.324307 | 4.3:-22681359 | MS.gene052565:CDS |
| ATCGAAAGAGGAAGCAATTC+AGG | 0.325278 | 4.3:-22681626 | MS.gene052565:CDS |
| GGATAATGCTTGGGCTGTTT+CGG | 0.325581 | 4.3:-22681815 | MS.gene052565:CDS |
| GCAATTCAGGTGAACTCAAT+TGG | 0.339387 | 4.3:-22681613 | MS.gene052565:CDS |
| GAGGATGAAGATTGGTGATT+TGG | 0.340567 | 4.3:-22682142 | MS.gene052565:CDS |
| TTTCTCTTACGCTCTCGATC+AGG | 0.347009 | 4.3:-22682530 | MS.gene052565:CDS |
| AAGGTTGTTTGTGTTGTTGC+TGG | 0.349484 | 4.3:-22681967 | MS.gene052565:CDS |
| TTCGAAGAATATGACCATTT+CGG | 0.350085 | 4.3:+22681499 | None:intergenic |
| GATGTAGTTCATGTTCTTGT+TGG | 0.350353 | 4.3:-22682033 | MS.gene052565:CDS |
| ATGCTGTGTCGACGTTTATC+AGG | 0.351926 | 4.3:-22681657 | MS.gene052565:CDS |
| GATCCAGAAGAGGGAAATTC+AGG | 0.354609 | 4.3:-22681115 | MS.gene052565:CDS |
| TTCATTCAGAATATTGCCTT+TGG | 0.362418 | 4.3:-22681589 | MS.gene052565:CDS |
| TGATGAATGTAAAGGGGATT+TGG | 0.369016 | 4.3:-22681752 | MS.gene052565:CDS |
| GATGAAATTGACGGAGAATT+CGG | 0.371164 | 4.3:-22681836 | MS.gene052565:CDS |
| CATTGCTTTCTTAGCTTCTT+CGG | 0.371612 | 4.3:+22681303 | None:intergenic |
| TTTGGATTGTGGGAGTGAGT+TGG | 0.372760 | 4.3:-22681884 | MS.gene052565:CDS |
| GGTTAAGAGGTTTATGGTTG+AGG | 0.384772 | 4.3:-22681683 | MS.gene052565:CDS |
| GATAAATTCTGCCATAAAAC+CGG | 0.390006 | 4.3:+22681273 | None:intergenic |
| GTGTTATCGCACCTTTGATC+CGG | 0.390866 | 4.3:-22681909 | MS.gene052565:CDS |
| AGGATGAAGATTGGTGATTT+GGG | 0.392679 | 4.3:-22682141 | MS.gene052565:CDS |
| ATCATTTGAAGTTCGCGAAA+TGG | 0.400579 | 4.3:-22681233 | MS.gene052565:CDS |
| CAAGCTTGAAGAGCTTCATT+CGG | 0.407539 | 4.3:-22682475 | MS.gene052565:CDS |
| GCTTGGGCTGTTTCGGCTCA+TGG | 0.409210 | 4.3:-22681808 | MS.gene052565:CDS |
| GGTGAAGGAGTGTCACGATC+TGG | 0.410395 | 4.3:-22682376 | MS.gene052565:CDS |
| GATTTGGTTGGTCCTGCTTG+TGG | 0.412391 | 4.3:-22681736 | MS.gene052565:CDS |
| CTTTCGGAGATTTATAGAAC+GGG | 0.416233 | 4.3:-22682258 | MS.gene052565:CDS |
| TTGCTATATTATGTTCGAAA+TGG | 0.417702 | 4.3:-22681382 | MS.gene052565:CDS |
| TGCGTTGATTTCTCGTATAG+TGG | 0.420344 | 4.3:-22682345 | MS.gene052565:CDS |
| CGGAGAATTCGGATAATGCT+TGG | 0.427171 | 4.3:-22681825 | MS.gene052565:CDS |
| TGGTTTAGCGAAGAAGCTTT+CGG | 0.433272 | 4.3:-22682274 | MS.gene052565:CDS |
| CGGCGTTGTTGAATATTTGT+GGG | 0.439542 | 4.3:-22681777 | MS.gene052565:CDS |
| ATGTAGTTCATGTTCTTGTT+GGG | 0.447382 | 4.3:-22682032 | MS.gene052565:CDS |
| TGAGGATGAGAAGTATGTTA+AGG | 0.450602 | 4.3:-22682076 | MS.gene052565:CDS |
| GCCGCCGAGAATAGTGATTC+CGG | 0.451485 | 4.3:-22682444 | MS.gene052565:CDS |
| CCTTTGGAGATGAGTTGGTT+AGG | 0.452790 | 4.3:-22681573 | MS.gene052565:CDS |
| GGCACTCTCTGGTATGGTCT+TGG | 0.458623 | 4.3:-22681203 | MS.gene052565:CDS |
| CTCGCCGGAATCACTATTCT+CGG | 0.461302 | 4.3:+22682440 | None:intergenic |
| GTGGGAATGATGAATGTAAA+GGG | 0.463553 | 4.3:-22681759 | MS.gene052565:CDS |
| CAATTCAGGTGAACTCAATT+GGG | 0.464924 | 4.3:-22681612 | MS.gene052565:CDS |
| TGTTATCGCACCTTTGATCC+GGG | 0.465836 | 4.3:-22681908 | MS.gene052565:CDS |
| TCGCCGGAGTCGGCAAGCTC+CGG | 0.466984 | 4.3:+22682578 | None:intergenic |
| GCAGTATGTTGAATGGAATC+TGG | 0.472173 | 4.3:-22680925 | MS.gene052565:CDS |
| TTCTCTTACGCTCTCGATCA+GGG | 0.479381 | 4.3:-22682529 | MS.gene052565:CDS |
| TCCGGCCAGTTGAGATTCTC+CGG | 0.480015 | 4.3:+22682608 | None:intergenic |
| CCGGAGTCGGCAAGCTCCGG+TGG | 0.480353 | 4.3:+22682581 | None:intergenic |
| TTTGGTTGGTCCTGCTTGTG+GGG | 0.481375 | 4.3:-22681734 | MS.gene052565:CDS |
| GCGAGAATCCGTCGCTTTCG+AGG | 0.481653 | 4.3:-22682422 | MS.gene052565:CDS |
| TTGGTGTTGAGGAGGTTAAG+AGG | 0.482711 | 4.3:-22681696 | MS.gene052565:CDS |
| GTGCTTGTAAAGAGGATATG+AGG | 0.483614 | 4.3:-22682188 | MS.gene052565:CDS |
| TAAGAGGTTTATGGTTGAGG+AGG | 0.487395 | 4.3:-22681680 | MS.gene052565:CDS |
| TATTGCCTTTGGAGATGAGT+TGG | 0.493487 | 4.3:-22681578 | MS.gene052565:CDS |
| ACGGCGTTGTTGAATATTTG+TGG | 0.494941 | 4.3:-22681778 | MS.gene052565:CDS |
| ATGGCAGCCGAGGCACTCTC+TGG | 0.498131 | 4.3:-22681214 | MS.gene052565:CDS |
| TCTTCTTGAGGTTGTTGTTG+AGG | 0.499086 | 4.3:-22682094 | MS.gene052565:CDS |
| AAGCTTGAAGAGCTTCATTC+GGG | 0.499276 | 4.3:-22682474 | MS.gene052565:CDS |
| ATGGGTGATGAAGAGTTAAC+CGG | 0.499705 | 4.3:-22682627 | MS.gene052565:CDS |
| AGCCGAGGCACTCTCTGGTA+TGG | 0.501064 | 4.3:-22681209 | MS.gene052565:CDS |
| GAATCCGTCGCTTTCGAGGT+TGG | 0.503145 | 4.3:-22682418 | MS.gene052565:CDS |
| TGGATTGTGGGAGTGAGTTG+GGG | 0.503829 | 4.3:-22681882 | MS.gene052565:CDS |
| TCAAGAATTGGCTTTGAAAG+TGG | 0.504838 | 4.3:-22681347 | MS.gene052565:CDS |
| GGGGTGTTGAGAAATCTTGT+TGG | 0.506061 | 4.3:-22681715 | MS.gene052565:CDS |
| GTTGTTTCAAAACCGTGTCT+TGG | 0.507347 | 4.3:-22682210 | MS.gene052565:CDS |
| AGATGAGTTGGTTAGGCAAA+TGG | 0.509589 | 4.3:-22681566 | MS.gene052565:CDS |
| GCAAGCTCCGGTGGGAACTC+CGG | 0.510787 | 4.3:+22682590 | None:intergenic |
| CATACTGCGGAATCTGTTTG+TGG | 0.512597 | 4.3:+22680940 | None:intergenic |
| GAAGAAGCTAAGAAAGCAAT+GGG | 0.516697 | 4.3:-22681301 | MS.gene052565:CDS |
| GTATGAAGAAACAAGCTTTG+AGG | 0.522589 | 4.3:-22682119 | MS.gene052565:CDS |
| AGATTCCGCAGTATGTTGAA+TGG | 0.523644 | 4.3:-22680932 | MS.gene052565:CDS |
| GGAGAATTCGGATAATGCTT+GGG | 0.524081 | 4.3:-22681824 | MS.gene052565:CDS |
| GAGAAATCTTGTTGGTGTTG+AGG | 0.526183 | 4.3:-22681707 | MS.gene052565:CDS |
| GTGAAGGAGTGTCACGATCT+GGG | 0.535040 | 4.3:-22682375 | MS.gene052565:CDS |
| CTCTCGATCAGGGTCTTCGC+CGG | 0.535256 | 4.3:-22682519 | MS.gene052565:CDS |
| CAAATGGTGATTAGAGAAGG+TGG | 0.535384 | 4.3:-22681550 | MS.gene052565:CDS |
| CCACCGGAGCTTGCCGACTC+CGG | 0.541028 | 4.3:-22682581 | MS.gene052565:CDS |
| CAATCCAAAACCCGGATCAA+AGG | 0.542230 | 4.3:+22681898 | None:intergenic |
| AGCTTTGAGGAATCTTCTTG+AGG | 0.544272 | 4.3:-22682106 | MS.gene052565:CDS |
| AACAAAGGAAATAGCAATGA+GGG | 0.544827 | 4.3:-22681476 | MS.gene052565:CDS |
| TTCGGCTCATGGTGGTGTCA+CGG | 0.546469 | 4.3:-22681797 | MS.gene052565:CDS |
| GCGTTGATTTCTCGTATAGT+GGG | 0.552179 | 4.3:-22682344 | MS.gene052565:CDS |
| CACTCCCACAATCCAAAACC+CGG | 0.552372 | 4.3:+22681890 | None:intergenic |
| GTTAACCGGAGAATCTCAAC+TGG | 0.552559 | 4.3:-22682613 | MS.gene052565:CDS |
| GCTTTCGGAGATTTATAGAA+CGG | 0.553150 | 4.3:-22682259 | MS.gene052565:CDS |
| TGGGGAAAGTTGCGGCTGCA+AGG | 0.553228 | 4.3:-22681864 | MS.gene052565:CDS |
| GTAGCGTTTGCGAAACTCGA+TGG | 0.554998 | 4.3:-22682294 | MS.gene052565:CDS |
| CAGGCTTGTGAAATCGAAAG+AGG | 0.557597 | 4.3:-22681638 | MS.gene052565:CDS |
| AAGAAAGCAATGGGAGATGC+CGG | 0.558547 | 4.3:-22681292 | MS.gene052565:CDS |
| TGGTACCTAGAAATAGAAAG+AGG | 0.563539 | 4.3:-22681183 | MS.gene052565:CDS |
| ACCGGAGAATCTCAACTGGC+CGG | 0.566586 | 4.3:-22682609 | MS.gene052565:CDS |
| CGAAGAAGCTAAGAAAGCAA+TGG | 0.567081 | 4.3:-22681302 | MS.gene052565:CDS |
| CTAAAATACGTAACAAAGCA+CGG | 0.571449 | 4.3:+22681524 | None:intergenic |
| GCTCCAAAGCTCGCCGGAGT+CGG | 0.571896 | 4.3:+22682568 | None:intergenic |
| TGTGGGAATGATGAATGTAA+AGG | 0.577026 | 4.3:-22681760 | MS.gene052565:CDS |
| GTGTCTTGGTGCTTGTAAAG+AGG | 0.579166 | 4.3:-22682196 | MS.gene052565:CDS |
| TTTCTCAACACCCCACAAGC+AGG | 0.582608 | 4.3:+22681724 | None:intergenic |
| AGGCAAATGGTGATTAGAGA+AGG | 0.583520 | 4.3:-22681553 | MS.gene052565:CDS |
| GAGTGAGTTGGGGAAAGTTG+CGG | 0.584048 | 4.3:-22681872 | MS.gene052565:CDS |
| AAATGAGCTCCAAAGCTCGC+CGG | 0.590302 | 4.3:+22682562 | None:intergenic |
| GCAATTGCTTGATCCAGAAG+AGG | 0.594505 | 4.3:-22681125 | MS.gene052565:CDS |
| CGAAAGCGACGGATTCTCGC+CGG | 0.595097 | 4.3:+22682425 | None:intergenic |
| CAACTGGCCGGAGTTCCCAC+CGG | 0.596842 | 4.3:-22682597 | MS.gene052565:CDS |
| TGACAAGTTGCAATAGTGCA+AGG | 0.601203 | 4.3:-22681057 | MS.gene052565:CDS |
| GTCATATTCTTCGAAAACAA+AGG | 0.603999 | 4.3:-22681491 | MS.gene052565:CDS |
| CAATTGCTTGATCCAGAAGA+GGG | 0.611533 | 4.3:-22681124 | MS.gene052565:CDS |
| TTCAATAGTAGCGACGGTGA+AGG | 0.620702 | 4.3:-22682391 | MS.gene052565:CDS |
| AAATCTTGTTGGTGTTGAGG+AGG | 0.623752 | 4.3:-22681704 | MS.gene052565:CDS |
| CGGAGTCGGCAAGCTCCGGT+GGG | 0.624184 | 4.3:+22682582 | None:intergenic |
| CCTAACCAACTCATCTCCAA+AGG | 0.624383 | 4.3:+22681573 | None:intergenic |
| AATTCAAGAAGAGAGTGCTA+AGG | 0.633225 | 4.3:-22681986 | MS.gene052565:CDS |
| TCTTTACAAGCACCAAGACA+CGG | 0.634229 | 4.3:+22682198 | None:intergenic |
| AGTTCGCGAAATGGCAGCCG+AGG | 0.636169 | 4.3:-22681224 | MS.gene052565:CDS |
| TGGGAATGATGAATGTAAAG+GGG | 0.637553 | 4.3:-22681758 | MS.gene052565:CDS |
| GACCATACCAGAGAGTGCCT+CGG | 0.642714 | 4.3:+22681207 | None:intergenic |
| GAATGTAAAGGGGATTTGGT+TGG | 0.646471 | 4.3:-22681748 | MS.gene052565:CDS |
| GGTAACTTCAATAGTAGCGA+CGG | 0.649152 | 4.3:-22682397 | MS.gene052565:CDS |
| AAACAAAGGAAATAGCAATG+AGG | 0.655890 | 4.3:-22681477 | MS.gene052565:CDS |
| TTTCGGAGATTTATAGAACG+GGG | 0.658353 | 4.3:-22682257 | MS.gene052565:CDS |
| AAGGTGTTTGATGAAATTGA+CGG | 0.664534 | 4.3:-22681845 | MS.gene052565:CDS |
| GATCAAAGGTGCGATAACAC+CGG | 0.665102 | 4.3:+22681912 | None:intergenic |
| GTTACCAACCTCGAAAGCGA+CGG | 0.667911 | 4.3:+22682414 | None:intergenic |
| TGGGCTGTTTCGGCTCATGG+TGG | 0.671537 | 4.3:-22681805 | MS.gene052565:CDS |
| AGATTCCATTCAACATACTG+CGG | 0.693690 | 4.3:+22680927 | None:intergenic |
| GCCGGAATCACTATTCTCGG+CGG | 0.730159 | 4.3:+22682443 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTAAGTATTTTAATGAGTTA+TGG | - | chr4.3:22682122-22682141 | MS.gene052565:CDS | 20.0% |
| ! | TTGCTATATTATGTTCGAAA+TGG | - | chr4.3:22682158-22682177 | MS.gene052565:CDS | 25.0% |
| !!! | CTGGTTTTGATTCATATAAA+GGG | - | chr4.3:22681592-22681611 | MS.gene052565:CDS | 25.0% |
| !!! | TGGTTTTGATTCATATAAAG+GGG | - | chr4.3:22681593-22681612 | MS.gene052565:CDS | 25.0% |
| !!! | TTTAATGAGTTATGGTTTTG+TGG | - | chr4.3:22682130-22682149 | MS.gene052565:CDS | 25.0% |
| AAACAAAGGAAATAGCAATG+AGG | - | chr4.3:22682063-22682082 | MS.gene052565:CDS | 30.0% | |
| AAAGTGGCATTTAGATTATC+TGG | - | chr4.3:22682209-22682228 | MS.gene052565:CDS | 30.0% | |
| AACAAAGGAAATAGCAATGA+GGG | - | chr4.3:22682064-22682083 | MS.gene052565:CDS | 30.0% | |
| AGGAAAAAGATTGTTAGTTC+TGG | - | chr4.3:22682503-22682522 | MS.gene052565:CDS | 30.0% | |
| ATGTAGTTCATGTTCTTGTT+GGG | - | chr4.3:22681508-22681527 | MS.gene052565:CDS | 30.0% | |
| CTAAAATACGTAACAAAGCA+CGG | + | chr4.3:22682019-22682038 | None:intergenic | 30.0% | |
| GATAAATTCTGCCATAAAAC+CGG | + | chr4.3:22682270-22682289 | None:intergenic | 30.0% | |
| GTCATATTCTTCGAAAACAA+AGG | - | chr4.3:22682049-22682068 | MS.gene052565:CDS | 30.0% | |
| TGAAGTTTCGATTCAAGAAT+TGG | - | chr4.3:22682181-22682200 | MS.gene052565:CDS | 30.0% | |
| TTCATTCAGAATATTGCCTT+TGG | - | chr4.3:22681951-22681970 | MS.gene052565:CDS | 30.0% | |
| TTCGAAGAATATGACCATTT+CGG | + | chr4.3:22682044-22682063 | None:intergenic | 30.0% | |
| ! | AAGGTGTTTGATGAAATTGA+CGG | - | chr4.3:22681695-22681714 | MS.gene052565:CDS | 30.0% |
| ! | ACAAACCTCTTTCTATTTCT+AGG | + | chr4.3:22682365-22682384 | None:intergenic | 30.0% |
| !! | TACGTATTTTAGATCCGAAA+TGG | - | chr4.3:22682027-22682046 | MS.gene052565:CDS | 30.0% |
| !! | TGCTAGTTTGTCTATGTTTT+TGG | + | chr4.3:22682534-22682553 | None:intergenic | 30.0% |
| !!! | GCTGGTTTTGATTCATATAA+AGG | - | chr4.3:22681591-22681610 | MS.gene052565:CDS | 30.0% |
| AGATTCCATTCAACATACTG+CGG | + | chr4.3:22682616-22682635 | None:intergenic | 35.0% | |
| CAATTCAGGTGAACTCAATT+GGG | - | chr4.3:22681928-22681947 | MS.gene052565:CDS | 35.0% | |
| CATTGCTTTCTTAGCTTCTT+CGG | + | chr4.3:22682240-22682259 | None:intergenic | 35.0% | |
| CTTTCGGAGATTTATAGAAC+GGG | - | chr4.3:22681282-22681301 | MS.gene052565:CDS | 35.0% | |
| GAAGAAGCTAAGAAAGCAAT+GGG | - | chr4.3:22682239-22682258 | MS.gene052565:CDS | 35.0% | |
| GATGAAATTGACGGAGAATT+CGG | - | chr4.3:22681704-22681723 | MS.gene052565:CDS | 35.0% | |
| GATGTAGTTCATGTTCTTGT+TGG | - | chr4.3:22681507-22681526 | MS.gene052565:CDS | 35.0% | |
| GCTTTCGGAGATTTATAGAA+CGG | - | chr4.3:22681281-22681300 | MS.gene052565:CDS | 35.0% | |
| GTATGAAGAAACAAGCTTTG+AGG | - | chr4.3:22681421-22681440 | MS.gene052565:CDS | 35.0% | |
| GTGGGAATGATGAATGTAAA+GGG | - | chr4.3:22681781-22681800 | MS.gene052565:CDS | 35.0% | |
| TGATGAATGTAAAGGGGATT+TGG | - | chr4.3:22681788-22681807 | MS.gene052565:CDS | 35.0% | |
| TGGGAATGATGAATGTAAAG+GGG | - | chr4.3:22681782-22681801 | MS.gene052565:CDS | 35.0% | |
| TGGTACCTAGAAATAGAAAG+AGG | - | chr4.3:22682357-22682376 | MS.gene052565:CDS | 35.0% | |
| TGTGGGAATGATGAATGTAA+AGG | - | chr4.3:22681780-22681799 | MS.gene052565:CDS | 35.0% | |
| TTTCGGAGATTTATAGAACG+GGG | - | chr4.3:22681283-22681302 | MS.gene052565:CDS | 35.0% | |
| ! | AGGATGAAGATTGGTGATTT+GGG | - | chr4.3:22681399-22681418 | MS.gene052565:CDS | 35.0% |
| ! | ATCATTTGAAGTTCGCGAAA+TGG | - | chr4.3:22682307-22682326 | MS.gene052565:CDS | 35.0% |
| ! | CATGTTCTTGTTGGGTTTTT+GGG | - | chr4.3:22681516-22681535 | MS.gene052565:CDS | 35.0% |
| ! | TCATGTTCTTGTTGGGTTTT+TGG | - | chr4.3:22681515-22681534 | MS.gene052565:CDS | 35.0% |
| ! | TGAGGATGAGAAGTATGTTA+AGG | - | chr4.3:22681464-22681483 | MS.gene052565:CDS | 35.0% |
| !! | AATTCAAGAAGAGAGTGCTA+AGG | - | chr4.3:22681554-22681573 | MS.gene052565:CDS | 35.0% |
| !! | ATTTTGACGAGGATGAAGAT+TGG | - | chr4.3:22681390-22681409 | MS.gene052565:CDS | 35.0% |
| !! | TCAAGAATTGGCTTTGAAAG+TGG | - | chr4.3:22682193-22682212 | MS.gene052565:CDS | 35.0% |
| !!! | ACGAATAAGTTGCCATTTTC+CGG | + | chr4.3:22681043-22681062 | None:intergenic | 35.0% |
| !!! | ATGTGAGAGATATTTTGACG+AGG | - | chr4.3:22681379-22681398 | MS.gene052565:CDS | 35.0% |
| AAGCTTGAAGAGCTTCATTC+GGG | - | chr4.3:22681066-22681085 | MS.gene052565:CDS | 40.0% | |
| ACGGCGTTGTTGAATATTTG+TGG | - | chr4.3:22681762-22681781 | MS.gene052565:CDS | 40.0% | |
| AGATTCCGCAGTATGTTGAA+TGG | - | chr4.3:22682608-22682627 | MS.gene052565:CDS | 40.0% | |
| AGGCAAATGGTGATTAGAGA+AGG | - | chr4.3:22681987-22682006 | MS.gene052565:CDS | 40.0% | |
| ATCGAAAGAGGAAGCAATTC+AGG | - | chr4.3:22681914-22681933 | MS.gene052565:CDS | 40.0% | |
| ATGGGTGATGAAGAGTTAAC+CGG | - | chr4.3:22680913-22680932 | MS.gene052565:CDS | 40.0% | |
| CAAATGGTGATTAGAGAAGG+TGG | - | chr4.3:22681990-22682009 | MS.gene052565:CDS | 40.0% | |
| CAAGCTTGAAGAGCTTCATT+CGG | - | chr4.3:22681065-22681084 | MS.gene052565:CDS | 40.0% | |
| CAATTGCTTGATCCAGAAGA+GGG | - | chr4.3:22682416-22682435 | MS.gene052565:CDS | 40.0% | |
| CGAAGAAGCTAAGAAAGCAA+TGG | - | chr4.3:22682238-22682257 | MS.gene052565:CDS | 40.0% | |
| CGGCGTTGTTGAATATTTGT+GGG | - | chr4.3:22681763-22681782 | MS.gene052565:CDS | 40.0% | |
| GCAATTCAGGTGAACTCAAT+TGG | - | chr4.3:22681927-22681946 | MS.gene052565:CDS | 40.0% | |
| GCAGTATGTTGAATGGAATC+TGG | - | chr4.3:22682615-22682634 | MS.gene052565:CDS | 40.0% | |
| GGAGAATTCGGATAATGCTT+GGG | - | chr4.3:22681716-22681735 | MS.gene052565:CDS | 40.0% | |
| GGTAACTTCAATAGTAGCGA+CGG | - | chr4.3:22681143-22681162 | MS.gene052565:CDS | 40.0% | |
| GGTTAAGAGGTTTATGGTTG+AGG | - | chr4.3:22681857-22681876 | MS.gene052565:CDS | 40.0% | |
| GTGCTTGTAAAGAGGATATG+AGG | - | chr4.3:22681352-22681371 | MS.gene052565:CDS | 40.0% | |
| TAAGAGGTTTATGGTTGAGG+AGG | - | chr4.3:22681860-22681879 | MS.gene052565:CDS | 40.0% | |
| TCTTTACAAGCACCAAGACA+CGG | + | chr4.3:22681345-22681364 | None:intergenic | 40.0% | |
| TGACAAGTTGCAATAGTGCA+AGG | - | chr4.3:22682483-22682502 | MS.gene052565:CDS | 40.0% | |
| TGAGGAGGTTAAGAGGTTTA+TGG | - | chr4.3:22681851-22681870 | MS.gene052565:CDS | 40.0% | |
| TGGTTTAGCGAAGAAGCTTT+CGG | - | chr4.3:22681266-22681285 | MS.gene052565:CDS | 40.0% | |
| TTACCTGAATTTCCCTCTTC+TGG | + | chr4.3:22682431-22682450 | None:intergenic | 40.0% | |
| ! | AAGGTTGTTTGTGTTGTTGC+TGG | - | chr4.3:22681573-22681592 | MS.gene052565:CDS | 40.0% |
| ! | GAATGTAAAGGGGATTTGGT+TGG | - | chr4.3:22681792-22681811 | MS.gene052565:CDS | 40.0% |
| ! | GAGGATGAAGATTGGTGATT+TGG | - | chr4.3:22681398-22681417 | MS.gene052565:CDS | 40.0% |
| ! | GCTTTTGATGCAGAGTGATT+TGG | - | chr4.3:22681221-22681240 | MS.gene052565:CDS | 40.0% |
| ! | GTTGTTTCAAAACCGTGTCT+TGG | - | chr4.3:22681330-22681349 | MS.gene052565:CDS | 40.0% |
| ! | TATTGCCTTTGGAGATGAGT+TGG | - | chr4.3:22681962-22681981 | MS.gene052565:CDS | 40.0% |
| ! | TCTTCTTGAGGTTGTTGTTG+AGG | - | chr4.3:22681446-22681465 | MS.gene052565:CDS | 40.0% |
| !! | AAATCTTGTTGGTGTTGAGG+AGG | - | chr4.3:22681836-22681855 | MS.gene052565:CDS | 40.0% |
| !! | AGAACGGGGATTTTGACAAA+TGG | - | chr4.3:22681297-22681316 | MS.gene052565:CDS | 40.0% |
| !! | AGATGAGTTGGTTAGGCAAA+TGG | - | chr4.3:22681974-22681993 | MS.gene052565:CDS | 40.0% |
| !! | AGCTTTGAGGAATCTTCTTG+AGG | - | chr4.3:22681434-22681453 | MS.gene052565:CDS | 40.0% |
| !! | GAACGGGGATTTTGACAAAT+GGG | - | chr4.3:22681298-22681317 | MS.gene052565:CDS | 40.0% |
| !! | GAGAAATCTTGTTGGTGTTG+AGG | - | chr4.3:22681833-22681852 | MS.gene052565:CDS | 40.0% |
| !! | GCGTTGATTTCTCGTATAGT+GGG | - | chr4.3:22681196-22681215 | MS.gene052565:CDS | 40.0% |
| !! | TGCGTTGATTTCTCGTATAG+TGG | - | chr4.3:22681195-22681214 | MS.gene052565:CDS | 40.0% |
| !!! | AAAGGGGTTTTGATTAGTGC+CGG | - | chr4.3:22681609-22681628 | MS.gene052565:CDS | 40.0% |
| !!! | GTTTTTGGGTTCTAATGAGG+TGG | - | chr4.3:22681530-22681549 | MS.gene052565:CDS | 40.0% |
| !!! | TGGGTTTTTGGGTTCTAATG+AGG | - | chr4.3:22681527-22681546 | MS.gene052565:CDS | 40.0% |
| AAGAAAGCAATGGGAGATGC+CGG | - | chr4.3:22682248-22682267 | MS.gene052565:CDS | 45.0% | |
| CAATCCAAAACCCGGATCAA+AGG | + | chr4.3:22681645-22681664 | None:intergenic | 45.0% | |
| CAGGCTTGTGAAATCGAAAG+AGG | - | chr4.3:22681902-22681921 | MS.gene052565:CDS | 45.0% | |
| CATACTGCGGAATCTGTTTG+TGG | + | chr4.3:22682603-22682622 | None:intergenic | 45.0% | |
| CCTAACCAACTCATCTCCAA+AGG | + | chr4.3:22681970-22681989 | None:intergenic | 45.0% | |
| CGGAGAATTCGGATAATGCT+TGG | - | chr4.3:22681715-22681734 | MS.gene052565:CDS | 45.0% | |
| GATCCAGAAGAGGGAAATTC+AGG | - | chr4.3:22682425-22682444 | MS.gene052565:CDS | 45.0% | |
| GCAATTGCTTGATCCAGAAG+AGG | - | chr4.3:22682415-22682434 | MS.gene052565:CDS | 45.0% | |
| GGATAATGCTTGGGCTGTTT+CGG | - | chr4.3:22681725-22681744 | MS.gene052565:CDS | 45.0% | |
| GTGTTATCGCACCTTTGATC+CGG | - | chr4.3:22681631-22681650 | MS.gene052565:CDS | 45.0% | |
| GTTAACCGGAGAATCTCAAC+TGG | - | chr4.3:22680927-22680946 | MS.gene052565:CDS | 45.0% | |
| TGTTATCGCACCTTTGATCC+GGG | - | chr4.3:22681632-22681651 | MS.gene052565:CDS | 45.0% | |
| TTCAATAGTAGCGACGGTGA+AGG | - | chr4.3:22681149-22681168 | MS.gene052565:CDS | 45.0% | |
| TTCTCTTACGCTCTCGATCA+GGG | - | chr4.3:22681011-22681030 | MS.gene052565:CDS | 45.0% | |
| TTGGATTGTGGGAGTGAGTT+GGG | - | chr4.3:22681657-22681676 | MS.gene052565:CDS | 45.0% | |
| TTTCTCTTACGCTCTCGATC+AGG | - | chr4.3:22681010-22681029 | MS.gene052565:CDS | 45.0% | |
| TTTGGATTGTGGGAGTGAGT+TGG | - | chr4.3:22681656-22681675 | MS.gene052565:CDS | 45.0% | |
| ! | ATGCTGTGTCGACGTTTATC+AGG | - | chr4.3:22681883-22681902 | MS.gene052565:CDS | 45.0% |
| ! | ATTTGGTTGGTCCTGCTTGT+GGG | - | chr4.3:22681805-22681824 | MS.gene052565:CDS | 45.0% |
| ! | GATCAAAGGTGCGATAACAC+CGG | + | chr4.3:22681631-22681650 | None:intergenic | 45.0% |
| !! | CCTTTGGAGATGAGTTGGTT+AGG | - | chr4.3:22681967-22681986 | MS.gene052565:CDS | 45.0% |
| !! | GGGGTGTTGAGAAATCTTGT+TGG | - | chr4.3:22681825-22681844 | MS.gene052565:CDS | 45.0% |
| !! | GTGTCTTGGTGCTTGTAAAG+AGG | - | chr4.3:22681344-22681363 | MS.gene052565:CDS | 45.0% |
| !! | TTGGTGTTGAGGAGGTTAAG+AGG | - | chr4.3:22681844-22681863 | MS.gene052565:CDS | 45.0% |
| !!! | AATGGGAGATGCCGGTTTTA+TGG | - | chr4.3:22682256-22682275 | MS.gene052565:CDS | 45.0% |
| !!! | TGATCCGGGTTTTGGATTGT+GGG | - | chr4.3:22681646-22681665 | MS.gene052565:CDS | 45.0% |
| !!! | TTGATCCGGGTTTTGGATTG+TGG | - | chr4.3:22681645-22681664 | MS.gene052565:CDS | 45.0% |
| AAATGAGCTCCAAAGCTCGC+CGG | + | chr4.3:22680981-22681000 | None:intergenic | 50.0% | |
| CACTCCCACAATCCAAAACC+CGG | + | chr4.3:22681653-22681672 | None:intergenic | 50.0% | |
| CTCGCCGGAATCACTATTCT+CGG | + | chr4.3:22681103-22681122 | None:intergenic | 50.0% | |
| GTAGCGTTTGCGAAACTCGA+TGG | - | chr4.3:22681246-22681265 | MS.gene052565:CDS | 50.0% | |
| GTTACCAACCTCGAAAGCGA+CGG | + | chr4.3:22681129-22681148 | None:intergenic | 50.0% | |
| TGGATTGTGGGAGTGAGTTG+GGG | - | chr4.3:22681658-22681677 | MS.gene052565:CDS | 50.0% | |
| TTTCTCAACACCCCACAAGC+AGG | + | chr4.3:22681819-22681838 | None:intergenic | 50.0% | |
| ! | GAGTGAGTTGGGGAAAGTTG+CGG | - | chr4.3:22681668-22681687 | MS.gene052565:CDS | 50.0% |
| ! | GATTTGGTTGGTCCTGCTTG+TGG | - | chr4.3:22681804-22681823 | MS.gene052565:CDS | 50.0% |
| ! | TTTGGTTGGTCCTGCTTGTG+GGG | - | chr4.3:22681806-22681825 | MS.gene052565:CDS | 50.0% |
| !! | GTGAAGGAGTGTCACGATCT+GGG | - | chr4.3:22681165-22681184 | MS.gene052565:CDS | 50.0% |
| ACCGGAGAATCTCAACTGGC+CGG | - | chr4.3:22680931-22680950 | MS.gene052565:CDS | 55.0% | |
| GCCGCCGAGAATAGTGATTC+CGG | - | chr4.3:22681096-22681115 | MS.gene052565:CDS | 55.0% | |
| GCCGGAATCACTATTCTCGG+CGG | + | chr4.3:22681100-22681119 | None:intergenic | 55.0% | |
| GGCACTCTCTGGTATGGTCT+TGG | - | chr4.3:22682337-22682356 | MS.gene052565:CDS | 55.0% | |
| TCAGGGTCTTCGCCGGAAAA+TGG | - | chr4.3:22681028-22681047 | MS.gene052565:CDS | 55.0% | |
| TCCGGCCAGTTGAGATTCTC+CGG | + | chr4.3:22680935-22680954 | None:intergenic | 55.0% | |
| ! | CGCACCTTTGATCCGGGTTT+TGG | - | chr4.3:22681638-22681657 | MS.gene052565:CDS | 55.0% |
| ! | GAATCCGTCGCTTTCGAGGT+TGG | - | chr4.3:22681122-22681141 | MS.gene052565:CDS | 55.0% |
| ! | TTCGGCTCATGGTGGTGTCA+CGG | - | chr4.3:22681743-22681762 | MS.gene052565:CDS | 55.0% |
| !! | GACCATACCAGAGAGTGCCT+CGG | + | chr4.3:22682336-22682355 | None:intergenic | 55.0% |
| !! | GGTGAAGGAGTGTCACGATC+TGG | - | chr4.3:22681164-22681183 | MS.gene052565:CDS | 55.0% |
| AGTTCGCGAAATGGCAGCCG+AGG | - | chr4.3:22682316-22682335 | MS.gene052565:CDS | 60.0% | |
| CTCTCGATCAGGGTCTTCGC+CGG | - | chr4.3:22681021-22681040 | MS.gene052565:CDS | 60.0% | |
| TGGGGAAAGTTGCGGCTGCA+AGG | - | chr4.3:22681676-22681695 | MS.gene052565:CDS | 60.0% | |
| ! | AGCCGAGGCACTCTCTGGTA+TGG | - | chr4.3:22682331-22682350 | MS.gene052565:CDS | 60.0% |
| ! | CGAAAGCGACGGATTCTCGC+CGG | + | chr4.3:22681118-22681137 | None:intergenic | 60.0% |
| ! | GCGAGAATCCGTCGCTTTCG+AGG | - | chr4.3:22681118-22681137 | MS.gene052565:CDS | 60.0% |
| ! | GCTTGGGCTGTTTCGGCTCA+TGG | - | chr4.3:22681732-22681751 | MS.gene052565:CDS | 60.0% |
| ! | TGGGCTGTTTCGGCTCATGG+TGG | - | chr4.3:22681735-22681754 | MS.gene052565:CDS | 60.0% |
| CAACTGGCCGGAGTTCCCAC+CGG | - | chr4.3:22680943-22680962 | MS.gene052565:CDS | 65.0% | |
| GCAAGCTCCGGTGGGAACTC+CGG | + | chr4.3:22680953-22680972 | None:intergenic | 65.0% | |
| GCTCCAAAGCTCGCCGGAGT+CGG | + | chr4.3:22680975-22680994 | None:intergenic | 65.0% | |
| ! | ATGGCAGCCGAGGCACTCTC+TGG | - | chr4.3:22682326-22682345 | MS.gene052565:CDS | 65.0% |
| !! | TTGCCGACTCCGGCGAGCTT+TGG | - | chr4.3:22680969-22680988 | MS.gene052565:CDS | 65.0% |
| CGGAGTCGGCAAGCTCCGGT+GGG | + | chr4.3:22680961-22680980 | None:intergenic | 70.0% | |
| TCGCCGGAGTCGGCAAGCTC+CGG | + | chr4.3:22680965-22680984 | None:intergenic | 70.0% | |
| !! | CCACCGGAGCTTGCCGACTC+CGG | - | chr4.3:22680959-22680978 | MS.gene052565:CDS | 70.0% |
| CCGGAGTCGGCAAGCTCCGG+TGG | + | chr4.3:22680962-22680981 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 22680913 | 22682649 | 22680913 | ID=MS.gene052565 |
| chr4.3 | mRNA | 22680913 | 22682649 | 22680913 | ID=MS.gene052565.t1;Parent=MS.gene052565 |
| chr4.3 | exon | 22680913 | 22682649 | 22680913 | ID=MS.gene052565.t1.exon1;Parent=MS.gene052565.t1 |
| chr4.3 | CDS | 22680913 | 22682649 | 22680913 | ID=cds.MS.gene052565.t1;Parent=MS.gene052565.t1 |
| Gene Sequence |
| Protein sequence |
