Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052564.t1 | XP_003608254.1 | 98.3 | 577 | 10 | 0 | 1 | 577 | 1 | 577 | 0.00E+00 | 1094 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052564.t1 | G7JRW0 | 98.3 | 577 | 10 | 0 | 1 | 577 | 1 | 577 | 0.0e+00 | 1094.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052564 | MS.gene052565 | 0.998263 | 3.04E-260 | -1.69E-46 |
| MS.gene052564 | MS.gene066900 | 0.822615 | 2.15E-53 | -1.69E-46 |
| MS.gene052564 | MS.gene067234 | 0.814722 | 1.33E-51 | -1.69E-46 |
| MS.gene052564 | MS.gene30964 | 0.811667 | 6.24E-51 | -1.69E-46 |
| MS.gene052564 | MS.gene32213 | 0.986589 | 2.69E-167 | -1.69E-46 |
| MS.gene052564 | MS.gene33842 | 0.998251 | 6.49E-260 | -1.69E-46 |
| MS.gene052564 | MS.gene45498 | 0.814804 | 1.28E-51 | -1.69E-46 |
| MS.gene052564 | MS.gene48442 | -0.810143 | 1.33E-50 | -1.69E-46 |
| MS.gene052564 | MS.gene49330 | 0.807158 | 5.79E-50 | -1.69E-46 |
| MS.gene052564 | MS.gene50595 | 0.812391 | 4.34E-51 | -1.69E-46 |
| MS.gene052564 | MS.gene57920 | 0.802908 | 4.49E-49 | -1.69E-46 |
| MS.gene052564 | MS.gene59901 | 0.80597 | 1.03E-49 | -1.69E-46 |
| MS.gene052564 | MS.gene62431 | 0.808018 | 3.81E-50 | -1.69E-46 |
| MS.gene052564 | MS.gene70223 | -0.801265 | 9.77E-49 | -1.69E-46 |
| MS.gene052564 | MS.gene85443 | 0.804109 | 2.53E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene013180 | MS.gene052564 | PPI |
| MS.gene36899 | MS.gene052564 | PPI |
| MS.gene06253 | MS.gene052564 | PPI |
| MS.gene023795 | MS.gene052564 | PPI |
| MS.gene27647 | MS.gene052564 | PPI |
| MS.gene57486 | MS.gene052564 | PPI |
| MS.gene41659 | MS.gene052564 | PPI |
| MS.gene019466 | MS.gene052564 | PPI |
| MS.gene52172 | MS.gene052564 | PPI |
| MS.gene42235 | MS.gene052564 | PPI |
| MS.gene055531 | MS.gene052564 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052564.t1 | MTR_4g091320 | 98.267 | 577 | 10 | 0 | 1 | 577 | 1 | 577 | 0.0 | 1151 |
| MS.gene052564.t1 | MTR_4g007850 | 90.683 | 161 | 12 | 1 | 417 | 577 | 365 | 522 | 1.53e-92 | 294 |
| MS.gene052564.t1 | MTR_8g032570 | 31.625 | 566 | 336 | 18 | 27 | 572 | 48 | 582 | 1.75e-67 | 229 |
| MS.gene052564.t1 | MTR_8g032570 | 31.625 | 566 | 336 | 18 | 27 | 572 | 25 | 559 | 2.08e-67 | 229 |
| MS.gene052564.t1 | MTR_7g076220 | 30.526 | 570 | 345 | 15 | 22 | 570 | 17 | 556 | 3.04e-65 | 223 |
| MS.gene052564.t1 | MTR_3g098730 | 29.050 | 537 | 356 | 14 | 38 | 567 | 29 | 547 | 2.00e-45 | 169 |
| MS.gene052564.t1 | MTR_1g018180 | 23.318 | 223 | 165 | 3 | 201 | 423 | 103 | 319 | 7.40e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052564.t1 | AT1G61350 | 60.917 | 545 | 203 | 6 | 40 | 577 | 32 | 573 | 0.0 | 645 |
| MS.gene052564.t1 | AT2G45720 | 31.449 | 566 | 339 | 15 | 22 | 572 | 20 | 551 | 2.05e-69 | 234 |
| MS.gene052564.t1 | AT2G45720 | 31.449 | 566 | 339 | 15 | 22 | 572 | 20 | 551 | 2.05e-69 | 234 |
| MS.gene052564.t1 | AT2G45720 | 31.449 | 566 | 339 | 15 | 22 | 572 | 20 | 551 | 2.05e-69 | 234 |
| MS.gene052564.t1 | AT2G45720 | 31.449 | 566 | 339 | 15 | 22 | 572 | 20 | 551 | 2.05e-69 | 234 |
| MS.gene052564.t1 | AT2G45720 | 31.449 | 566 | 339 | 15 | 22 | 572 | 20 | 551 | 2.05e-69 | 234 |
| MS.gene052564.t1 | AT5G50900 | 31.588 | 554 | 354 | 12 | 23 | 567 | 11 | 548 | 1.97e-62 | 215 |
| MS.gene052564.t1 | AT1G01830 | 28.445 | 566 | 362 | 15 | 22 | 572 | 36 | 573 | 9.26e-59 | 206 |
| MS.gene052564.t1 | AT1G01830 | 28.445 | 566 | 362 | 15 | 22 | 572 | 36 | 573 | 9.26e-59 | 206 |
| MS.gene052564.t1 | AT1G01830 | 28.445 | 566 | 362 | 15 | 22 | 572 | 36 | 573 | 9.26e-59 | 206 |
| MS.gene052564.t1 | AT2G05810 | 29.853 | 546 | 358 | 16 | 38 | 571 | 40 | 572 | 1.24e-52 | 189 |
| MS.gene052564.t1 | AT2G05810 | 29.853 | 546 | 358 | 16 | 38 | 571 | 40 | 572 | 1.24e-52 | 189 |
| MS.gene052564.t1 | AT4G16490 | 24.451 | 319 | 217 | 8 | 143 | 447 | 157 | 465 | 7.80e-12 | 68.2 |
Find 131 sgRNAs with CRISPR-Local
Find 153 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACCTGAATTTCCCTCTTC+TGG | 0.157688 | 4.3:+22719563 | None:intergenic |
| CGCACCTTTGATCCGGGTTT+TGG | 0.178046 | 4.3:-22720353 | MS.gene052564:CDS |
| ACAAACCTCTTTCTATTTCT+AGG | 0.200258 | 4.3:+22719629 | None:intergenic |
| TGAGGAGGTTAAGAGGTTTA+TGG | 0.200986 | 4.3:-22720140 | MS.gene052564:CDS |
| TTGGATTGTGGGAGTGAGTT+GGG | 0.213740 | 4.3:-22720334 | MS.gene052564:CDS |
| AAAGTGGCATTTAGATTATC+TGG | 0.248155 | 4.3:-22719782 | MS.gene052564:CDS |
| ATTTGGTTGGTCCTGCTTGT+GGG | 0.301326 | 4.3:-22720186 | MS.gene052564:CDS |
| TCAGGGTCTTCGCCGGAAAA+TGG | 0.303092 | 4.3:-22720963 | MS.gene052564:CDS |
| TTGCCGACTCCGGCGAGCTT+TGG | 0.323964 | 4.3:-22721022 | MS.gene052564:CDS |
| TGAAGTTTCGATTCAAGAAT+TGG | 0.324307 | 4.3:-22719810 | MS.gene052564:CDS |
| ATCGAAAGAGGAAGCAATTC+AGG | 0.325278 | 4.3:-22720077 | MS.gene052564:CDS |
| GGATAATGCTTGGGCTGTTT+CGG | 0.325581 | 4.3:-22720266 | MS.gene052564:CDS |
| GCAATTCAGGTGAACTCAAT+TGG | 0.339387 | 4.3:-22720064 | MS.gene052564:CDS |
| GAGGATGAAGATTGGTGATT+TGG | 0.340567 | 4.3:-22720593 | MS.gene052564:CDS |
| TTTCTCTTACGCTCTCGATC+AGG | 0.347009 | 4.3:-22720981 | MS.gene052564:CDS |
| AAGGTTGTTTGTGTTGTTGC+TGG | 0.349484 | 4.3:-22720418 | MS.gene052564:CDS |
| TTCGAAGAATATGACCATTT+CGG | 0.350085 | 4.3:+22719950 | None:intergenic |
| GATGTAGTTCATGTTCTTGT+TGG | 0.350353 | 4.3:-22720484 | MS.gene052564:CDS |
| ATGCTGTGTCGACGTTTATC+AGG | 0.351926 | 4.3:-22720108 | MS.gene052564:CDS |
| GATCCAGAAGAGGGAAATTC+AGG | 0.354609 | 4.3:-22719566 | MS.gene052564:CDS |
| TTCATTCAGAATATTGCCTT+TGG | 0.362418 | 4.3:-22720040 | MS.gene052564:CDS |
| TGATGAATGTAAAGGGGATT+TGG | 0.369016 | 4.3:-22720203 | MS.gene052564:CDS |
| GATGAAATTGACGGAGAATT+CGG | 0.371164 | 4.3:-22720287 | MS.gene052564:CDS |
| CATTGCTTTCTTAGCTTCTT+CGG | 0.371612 | 4.3:+22719754 | None:intergenic |
| TTTGGATTGTGGGAGTGAGT+TGG | 0.372760 | 4.3:-22720335 | MS.gene052564:CDS |
| GGTTAAGAGGTTTATGGTTG+AGG | 0.384772 | 4.3:-22720134 | MS.gene052564:CDS |
| GATAAATTCTGCCATAAAAC+CGG | 0.390006 | 4.3:+22719724 | None:intergenic |
| GTGTTATCGCACCTTTGATC+CGG | 0.390866 | 4.3:-22720360 | MS.gene052564:CDS |
| AGGATGAAGATTGGTGATTT+GGG | 0.392679 | 4.3:-22720592 | MS.gene052564:CDS |
| ATCATTTGAAGTTCGCGAAA+TGG | 0.400579 | 4.3:-22719684 | MS.gene052564:CDS |
| CAAGCTTGAAGAGCTTCATT+CGG | 0.407539 | 4.3:-22720926 | MS.gene052564:CDS |
| GCTTGGGCTGTTTCGGCTCA+TGG | 0.409210 | 4.3:-22720259 | MS.gene052564:CDS |
| GGTGAAGGAGTGTCACGATC+TGG | 0.410395 | 4.3:-22720827 | MS.gene052564:CDS |
| GATTTGGTTGGTCCTGCTTG+TGG | 0.412391 | 4.3:-22720187 | MS.gene052564:CDS |
| CTTTCGGAGATTTATAGAAC+GGG | 0.416233 | 4.3:-22720709 | MS.gene052564:CDS |
| TTGCTATATTATGTTCGAAA+TGG | 0.417702 | 4.3:-22719833 | MS.gene052564:CDS |
| TGCGTTGATTTCTCGTATAG+TGG | 0.420344 | 4.3:-22720796 | MS.gene052564:CDS |
| CGGAGAATTCGGATAATGCT+TGG | 0.427171 | 4.3:-22720276 | MS.gene052564:CDS |
| TGGTTTAGCGAAGAAGCTTT+CGG | 0.433272 | 4.3:-22720725 | MS.gene052564:CDS |
| CGGCGTTGTTGAATATTTGT+GGG | 0.439542 | 4.3:-22720228 | MS.gene052564:CDS |
| ATGTAGTTCATGTTCTTGTT+GGG | 0.447382 | 4.3:-22720483 | MS.gene052564:CDS |
| TGAGGATGAGAAGTATGTTA+AGG | 0.450602 | 4.3:-22720527 | MS.gene052564:CDS |
| GCCGCCGAGAATAGTGATTC+CGG | 0.451485 | 4.3:-22720895 | MS.gene052564:CDS |
| CCTTTGGAGATGAGTTGGTT+AGG | 0.452790 | 4.3:-22720024 | MS.gene052564:CDS |
| GGCACTCTCTGGTATGGTCT+TGG | 0.458623 | 4.3:-22719654 | MS.gene052564:CDS |
| CTCGCCGGAATCACTATTCT+CGG | 0.461302 | 4.3:+22720891 | None:intergenic |
| GTGGGAATGATGAATGTAAA+GGG | 0.463553 | 4.3:-22720210 | MS.gene052564:CDS |
| CAATTCAGGTGAACTCAATT+GGG | 0.464924 | 4.3:-22720063 | MS.gene052564:CDS |
| TGTTATCGCACCTTTGATCC+GGG | 0.465836 | 4.3:-22720359 | MS.gene052564:CDS |
| TCGCCGGAGTCGGCAAGCTC+CGG | 0.466984 | 4.3:+22721029 | None:intergenic |
| GCAGTATGTTGAATGGAATC+TGG | 0.472173 | 4.3:-22719376 | MS.gene052564:CDS |
| TTCTCTTACGCTCTCGATCA+GGG | 0.479381 | 4.3:-22720980 | MS.gene052564:CDS |
| TCCGGCCAGTTGAGATTCTC+CGG | 0.480015 | 4.3:+22721059 | None:intergenic |
| CCGGAGTCGGCAAGCTCCGG+TGG | 0.480353 | 4.3:+22721032 | None:intergenic |
| TTTGGTTGGTCCTGCTTGTG+GGG | 0.481375 | 4.3:-22720185 | MS.gene052564:CDS |
| GCGAGAATCCGTCGCTTTCG+AGG | 0.481653 | 4.3:-22720873 | MS.gene052564:CDS |
| TTGGTGTTGAGGAGGTTAAG+AGG | 0.482711 | 4.3:-22720147 | MS.gene052564:CDS |
| GTGCTTGTAAAGAGGATATG+AGG | 0.483614 | 4.3:-22720639 | MS.gene052564:CDS |
| TAAGAGGTTTATGGTTGAGG+AGG | 0.487395 | 4.3:-22720131 | MS.gene052564:CDS |
| TATTGCCTTTGGAGATGAGT+TGG | 0.493487 | 4.3:-22720029 | MS.gene052564:CDS |
| ACGGCGTTGTTGAATATTTG+TGG | 0.494941 | 4.3:-22720229 | MS.gene052564:CDS |
| ATGGCAGCCGAGGCACTCTC+TGG | 0.498131 | 4.3:-22719665 | MS.gene052564:CDS |
| TCTTCTTGAGGTTGTTGTTG+AGG | 0.499086 | 4.3:-22720545 | MS.gene052564:CDS |
| AAGCTTGAAGAGCTTCATTC+GGG | 0.499276 | 4.3:-22720925 | MS.gene052564:CDS |
| ATGGGTGATGAAGAGTTAAC+CGG | 0.499705 | 4.3:-22721078 | MS.gene052564:CDS |
| AGCCGAGGCACTCTCTGGTA+TGG | 0.501064 | 4.3:-22719660 | MS.gene052564:CDS |
| GAATCCGTCGCTTTCGAGGT+TGG | 0.503145 | 4.3:-22720869 | MS.gene052564:CDS |
| TGGATTGTGGGAGTGAGTTG+GGG | 0.503829 | 4.3:-22720333 | MS.gene052564:CDS |
| TCAAGAATTGGCTTTGAAAG+TGG | 0.504838 | 4.3:-22719798 | MS.gene052564:CDS |
| GGGGTGTTGAGAAATCTTGT+TGG | 0.506061 | 4.3:-22720166 | MS.gene052564:CDS |
| GTTGTTTCAAAACCGTGTCT+TGG | 0.507347 | 4.3:-22720661 | MS.gene052564:CDS |
| AGATGAGTTGGTTAGGCAAA+TGG | 0.509589 | 4.3:-22720017 | MS.gene052564:CDS |
| GCAAGCTCCGGTGGGAACTC+CGG | 0.510787 | 4.3:+22721041 | None:intergenic |
| CATACTGCGGAATCTGTTTG+TGG | 0.512597 | 4.3:+22719391 | None:intergenic |
| GAAGAAGCTAAGAAAGCAAT+GGG | 0.516697 | 4.3:-22719752 | MS.gene052564:CDS |
| GTATGAAGAAACAAGCTTTG+AGG | 0.522589 | 4.3:-22720570 | MS.gene052564:CDS |
| AGATTCCGCAGTATGTTGAA+TGG | 0.523644 | 4.3:-22719383 | MS.gene052564:CDS |
| GGAGAATTCGGATAATGCTT+GGG | 0.524081 | 4.3:-22720275 | MS.gene052564:CDS |
| GAGAAATCTTGTTGGTGTTG+AGG | 0.526183 | 4.3:-22720158 | MS.gene052564:CDS |
| GTGAAGGAGTGTCACGATCT+GGG | 0.535040 | 4.3:-22720826 | MS.gene052564:CDS |
| CTCTCGATCAGGGTCTTCGC+CGG | 0.535256 | 4.3:-22720970 | MS.gene052564:CDS |
| CAAATGGTGATTAGAGAAGG+TGG | 0.535384 | 4.3:-22720001 | MS.gene052564:CDS |
| CCACCGGAGCTTGCCGACTC+CGG | 0.541028 | 4.3:-22721032 | MS.gene052564:CDS |
| CAATCCAAAACCCGGATCAA+AGG | 0.542230 | 4.3:+22720349 | None:intergenic |
| AGCTTTGAGGAATCTTCTTG+AGG | 0.544272 | 4.3:-22720557 | MS.gene052564:CDS |
| AACAAAGGAAATAGCAATGA+GGG | 0.544827 | 4.3:-22719927 | MS.gene052564:CDS |
| TTCGGCTCATGGTGGTGTCA+CGG | 0.546469 | 4.3:-22720248 | MS.gene052564:CDS |
| GCGTTGATTTCTCGTATAGT+GGG | 0.552179 | 4.3:-22720795 | MS.gene052564:CDS |
| CACTCCCACAATCCAAAACC+CGG | 0.552372 | 4.3:+22720341 | None:intergenic |
| GTTAACCGGAGAATCTCAAC+TGG | 0.552559 | 4.3:-22721064 | MS.gene052564:CDS |
| GCTTTCGGAGATTTATAGAA+CGG | 0.553150 | 4.3:-22720710 | MS.gene052564:CDS |
| TGGGGAAAGTTGCGGCTGCA+AGG | 0.553228 | 4.3:-22720315 | MS.gene052564:CDS |
| GTAGCGTTTGCGAAACTCGA+TGG | 0.554998 | 4.3:-22720745 | MS.gene052564:CDS |
| CAGGCTTGTGAAATCGAAAG+AGG | 0.557597 | 4.3:-22720089 | MS.gene052564:CDS |
| AAGAAAGCAATGGGAGATGC+CGG | 0.558547 | 4.3:-22719743 | MS.gene052564:CDS |
| TGGTACCTAGAAATAGAAAG+AGG | 0.563539 | 4.3:-22719634 | MS.gene052564:CDS |
| ACCGGAGAATCTCAACTGGC+CGG | 0.566586 | 4.3:-22721060 | MS.gene052564:CDS |
| CGAAGAAGCTAAGAAAGCAA+TGG | 0.567081 | 4.3:-22719753 | MS.gene052564:CDS |
| CTAAAATACGTAACAAAGCA+CGG | 0.571449 | 4.3:+22719975 | None:intergenic |
| GCTCCAAAGCTCGCCGGAGT+CGG | 0.571896 | 4.3:+22721019 | None:intergenic |
| TGTGGGAATGATGAATGTAA+AGG | 0.577026 | 4.3:-22720211 | MS.gene052564:CDS |
| GTGTCTTGGTGCTTGTAAAG+AGG | 0.579166 | 4.3:-22720647 | MS.gene052564:CDS |
| TTTCTCAACACCCCACAAGC+AGG | 0.582608 | 4.3:+22720175 | None:intergenic |
| AGGCAAATGGTGATTAGAGA+AGG | 0.583520 | 4.3:-22720004 | MS.gene052564:CDS |
| GAGTGAGTTGGGGAAAGTTG+CGG | 0.584048 | 4.3:-22720323 | MS.gene052564:CDS |
| AAATGAGCTCCAAAGCTCGC+CGG | 0.590302 | 4.3:+22721013 | None:intergenic |
| GCAATTGCTTGATCCAGAAG+AGG | 0.594505 | 4.3:-22719576 | MS.gene052564:CDS |
| CGAAAGCGACGGATTCTCGC+CGG | 0.595097 | 4.3:+22720876 | None:intergenic |
| CAACTGGCCGGAGTTCCCAC+CGG | 0.596842 | 4.3:-22721048 | MS.gene052564:CDS |
| TGACAAGTTGCAATAGTGCA+AGG | 0.601203 | 4.3:-22719508 | MS.gene052564:CDS |
| GTCATATTCTTCGAAAACAA+AGG | 0.603999 | 4.3:-22719942 | MS.gene052564:CDS |
| CAATTGCTTGATCCAGAAGA+GGG | 0.611533 | 4.3:-22719575 | MS.gene052564:CDS |
| TTCAATAGTAGCGACGGTGA+AGG | 0.620702 | 4.3:-22720842 | MS.gene052564:CDS |
| AAATCTTGTTGGTGTTGAGG+AGG | 0.623752 | 4.3:-22720155 | MS.gene052564:CDS |
| CGGAGTCGGCAAGCTCCGGT+GGG | 0.624184 | 4.3:+22721033 | None:intergenic |
| CCTAACCAACTCATCTCCAA+AGG | 0.624383 | 4.3:+22720024 | None:intergenic |
| AATTCAAGAAGAGAGTGCTA+AGG | 0.633225 | 4.3:-22720437 | MS.gene052564:CDS |
| TCTTTACAAGCACCAAGACA+CGG | 0.634229 | 4.3:+22720649 | None:intergenic |
| AGTTCGCGAAATGGCAGCCG+AGG | 0.636169 | 4.3:-22719675 | MS.gene052564:CDS |
| TGGGAATGATGAATGTAAAG+GGG | 0.637553 | 4.3:-22720209 | MS.gene052564:CDS |
| GACCATACCAGAGAGTGCCT+CGG | 0.642714 | 4.3:+22719658 | None:intergenic |
| GAATGTAAAGGGGATTTGGT+TGG | 0.646471 | 4.3:-22720199 | MS.gene052564:CDS |
| GGTAACTTCAATAGTAGCGA+CGG | 0.649152 | 4.3:-22720848 | MS.gene052564:CDS |
| AAACAAAGGAAATAGCAATG+AGG | 0.655890 | 4.3:-22719928 | MS.gene052564:CDS |
| TTTCGGAGATTTATAGAACG+GGG | 0.658353 | 4.3:-22720708 | MS.gene052564:CDS |
| AAGGTGTTTGATGAAATTGA+CGG | 0.664534 | 4.3:-22720296 | MS.gene052564:CDS |
| GATCAAAGGTGCGATAACAC+CGG | 0.665102 | 4.3:+22720363 | None:intergenic |
| GTTACCAACCTCGAAAGCGA+CGG | 0.667911 | 4.3:+22720865 | None:intergenic |
| TGGGCTGTTTCGGCTCATGG+TGG | 0.671537 | 4.3:-22720256 | MS.gene052564:CDS |
| AGATTCCATTCAACATACTG+CGG | 0.693690 | 4.3:+22719378 | None:intergenic |
| GCCGGAATCACTATTCTCGG+CGG | 0.730159 | 4.3:+22720894 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTAAGTATTTTAATGAGTTA+TGG | - | chr4.3:22720573-22720592 | MS.gene052564:CDS | 20.0% |
| ! | TTGCTATATTATGTTCGAAA+TGG | - | chr4.3:22720609-22720628 | MS.gene052564:CDS | 25.0% |
| !!! | CTGGTTTTGATTCATATAAA+GGG | - | chr4.3:22720043-22720062 | MS.gene052564:CDS | 25.0% |
| !!! | TGGTTTTGATTCATATAAAG+GGG | - | chr4.3:22720044-22720063 | MS.gene052564:CDS | 25.0% |
| !!! | TTTAATGAGTTATGGTTTTG+TGG | - | chr4.3:22720581-22720600 | MS.gene052564:CDS | 25.0% |
| AAACAAAGGAAATAGCAATG+AGG | - | chr4.3:22720514-22720533 | MS.gene052564:CDS | 30.0% | |
| AAAGTGGCATTTAGATTATC+TGG | - | chr4.3:22720660-22720679 | MS.gene052564:CDS | 30.0% | |
| AACAAAGGAAATAGCAATGA+GGG | - | chr4.3:22720515-22720534 | MS.gene052564:CDS | 30.0% | |
| AGGAAAAAGATTGTTAGTTC+TGG | - | chr4.3:22720954-22720973 | MS.gene052564:CDS | 30.0% | |
| ATGTAGTTCATGTTCTTGTT+GGG | - | chr4.3:22719959-22719978 | MS.gene052564:CDS | 30.0% | |
| CTAAAATACGTAACAAAGCA+CGG | + | chr4.3:22720470-22720489 | None:intergenic | 30.0% | |
| GATAAATTCTGCCATAAAAC+CGG | + | chr4.3:22720721-22720740 | None:intergenic | 30.0% | |
| GTCATATTCTTCGAAAACAA+AGG | - | chr4.3:22720500-22720519 | MS.gene052564:CDS | 30.0% | |
| TGAAGTTTCGATTCAAGAAT+TGG | - | chr4.3:22720632-22720651 | MS.gene052564:CDS | 30.0% | |
| TTCATTCAGAATATTGCCTT+TGG | - | chr4.3:22720402-22720421 | MS.gene052564:CDS | 30.0% | |
| TTCGAAGAATATGACCATTT+CGG | + | chr4.3:22720495-22720514 | None:intergenic | 30.0% | |
| ! | AAGGTGTTTGATGAAATTGA+CGG | - | chr4.3:22720146-22720165 | MS.gene052564:CDS | 30.0% |
| ! | ACAAACCTCTTTCTATTTCT+AGG | + | chr4.3:22720816-22720835 | None:intergenic | 30.0% |
| !! | TACGTATTTTAGATCCGAAA+TGG | - | chr4.3:22720478-22720497 | MS.gene052564:CDS | 30.0% |
| !! | TGCTAGTTTGTCTATGTTTT+TGG | + | chr4.3:22720985-22721004 | None:intergenic | 30.0% |
| !!! | GCTGGTTTTGATTCATATAA+AGG | - | chr4.3:22720042-22720061 | MS.gene052564:CDS | 30.0% |
| AGATTCCATTCAACATACTG+CGG | + | chr4.3:22721067-22721086 | None:intergenic | 35.0% | |
| CAATTCAGGTGAACTCAATT+GGG | - | chr4.3:22720379-22720398 | MS.gene052564:CDS | 35.0% | |
| CATTGCTTTCTTAGCTTCTT+CGG | + | chr4.3:22720691-22720710 | None:intergenic | 35.0% | |
| CTTTCGGAGATTTATAGAAC+GGG | - | chr4.3:22719733-22719752 | MS.gene052564:CDS | 35.0% | |
| GAAGAAGCTAAGAAAGCAAT+GGG | - | chr4.3:22720690-22720709 | MS.gene052564:CDS | 35.0% | |
| GATGAAATTGACGGAGAATT+CGG | - | chr4.3:22720155-22720174 | MS.gene052564:CDS | 35.0% | |
| GATGTAGTTCATGTTCTTGT+TGG | - | chr4.3:22719958-22719977 | MS.gene052564:CDS | 35.0% | |
| GCTTTCGGAGATTTATAGAA+CGG | - | chr4.3:22719732-22719751 | MS.gene052564:CDS | 35.0% | |
| GTATGAAGAAACAAGCTTTG+AGG | - | chr4.3:22719872-22719891 | MS.gene052564:CDS | 35.0% | |
| GTGGGAATGATGAATGTAAA+GGG | - | chr4.3:22720232-22720251 | MS.gene052564:CDS | 35.0% | |
| TGATGAATGTAAAGGGGATT+TGG | - | chr4.3:22720239-22720258 | MS.gene052564:CDS | 35.0% | |
| TGGGAATGATGAATGTAAAG+GGG | - | chr4.3:22720233-22720252 | MS.gene052564:CDS | 35.0% | |
| TGGTACCTAGAAATAGAAAG+AGG | - | chr4.3:22720808-22720827 | MS.gene052564:CDS | 35.0% | |
| TGTGGGAATGATGAATGTAA+AGG | - | chr4.3:22720231-22720250 | MS.gene052564:CDS | 35.0% | |
| TTTCGGAGATTTATAGAACG+GGG | - | chr4.3:22719734-22719753 | MS.gene052564:CDS | 35.0% | |
| ! | AGGATGAAGATTGGTGATTT+GGG | - | chr4.3:22719850-22719869 | MS.gene052564:CDS | 35.0% |
| ! | ATCATTTGAAGTTCGCGAAA+TGG | - | chr4.3:22720758-22720777 | MS.gene052564:CDS | 35.0% |
| ! | CATGTTCTTGTTGGGTTTTT+GGG | - | chr4.3:22719967-22719986 | MS.gene052564:CDS | 35.0% |
| ! | TCATGTTCTTGTTGGGTTTT+TGG | - | chr4.3:22719966-22719985 | MS.gene052564:CDS | 35.0% |
| ! | TGAGGATGAGAAGTATGTTA+AGG | - | chr4.3:22719915-22719934 | MS.gene052564:CDS | 35.0% |
| !! | AATTCAAGAAGAGAGTGCTA+AGG | - | chr4.3:22720005-22720024 | MS.gene052564:CDS | 35.0% |
| !! | ATTTTGACGAGGATGAAGAT+TGG | - | chr4.3:22719841-22719860 | MS.gene052564:CDS | 35.0% |
| !! | TCAAGAATTGGCTTTGAAAG+TGG | - | chr4.3:22720644-22720663 | MS.gene052564:CDS | 35.0% |
| !!! | ACGAATAAGTTGCCATTTTC+CGG | + | chr4.3:22719494-22719513 | None:intergenic | 35.0% |
| !!! | ATGTGAGAGATATTTTGACG+AGG | - | chr4.3:22719830-22719849 | MS.gene052564:CDS | 35.0% |
| AAGCTTGAAGAGCTTCATTC+GGG | - | chr4.3:22719517-22719536 | MS.gene052564:CDS | 40.0% | |
| ACGGCGTTGTTGAATATTTG+TGG | - | chr4.3:22720213-22720232 | MS.gene052564:CDS | 40.0% | |
| AGATTCCGCAGTATGTTGAA+TGG | - | chr4.3:22721059-22721078 | MS.gene052564:CDS | 40.0% | |
| AGGCAAATGGTGATTAGAGA+AGG | - | chr4.3:22720438-22720457 | MS.gene052564:CDS | 40.0% | |
| ATCGAAAGAGGAAGCAATTC+AGG | - | chr4.3:22720365-22720384 | MS.gene052564:CDS | 40.0% | |
| ATGGGTGATGAAGAGTTAAC+CGG | - | chr4.3:22719364-22719383 | MS.gene052564:CDS | 40.0% | |
| CAAATGGTGATTAGAGAAGG+TGG | - | chr4.3:22720441-22720460 | MS.gene052564:CDS | 40.0% | |
| CAAGCTTGAAGAGCTTCATT+CGG | - | chr4.3:22719516-22719535 | MS.gene052564:CDS | 40.0% | |
| CAATTGCTTGATCCAGAAGA+GGG | - | chr4.3:22720867-22720886 | MS.gene052564:CDS | 40.0% | |
| CGAAGAAGCTAAGAAAGCAA+TGG | - | chr4.3:22720689-22720708 | MS.gene052564:CDS | 40.0% | |
| CGGCGTTGTTGAATATTTGT+GGG | - | chr4.3:22720214-22720233 | MS.gene052564:CDS | 40.0% | |
| GCAATTCAGGTGAACTCAAT+TGG | - | chr4.3:22720378-22720397 | MS.gene052564:CDS | 40.0% | |
| GCAGTATGTTGAATGGAATC+TGG | - | chr4.3:22721066-22721085 | MS.gene052564:CDS | 40.0% | |
| GGAGAATTCGGATAATGCTT+GGG | - | chr4.3:22720167-22720186 | MS.gene052564:CDS | 40.0% | |
| GGTAACTTCAATAGTAGCGA+CGG | - | chr4.3:22719594-22719613 | MS.gene052564:CDS | 40.0% | |
| GGTTAAGAGGTTTATGGTTG+AGG | - | chr4.3:22720308-22720327 | MS.gene052564:CDS | 40.0% | |
| GTGCTTGTAAAGAGGATATG+AGG | - | chr4.3:22719803-22719822 | MS.gene052564:CDS | 40.0% | |
| TAAGAGGTTTATGGTTGAGG+AGG | - | chr4.3:22720311-22720330 | MS.gene052564:CDS | 40.0% | |
| TCTTTACAAGCACCAAGACA+CGG | + | chr4.3:22719796-22719815 | None:intergenic | 40.0% | |
| TGACAAGTTGCAATAGTGCA+AGG | - | chr4.3:22720934-22720953 | MS.gene052564:CDS | 40.0% | |
| TGAGGAGGTTAAGAGGTTTA+TGG | - | chr4.3:22720302-22720321 | MS.gene052564:CDS | 40.0% | |
| TGGTTTAGCGAAGAAGCTTT+CGG | - | chr4.3:22719717-22719736 | MS.gene052564:CDS | 40.0% | |
| TTACCTGAATTTCCCTCTTC+TGG | + | chr4.3:22720882-22720901 | None:intergenic | 40.0% | |
| ! | AAGGTTGTTTGTGTTGTTGC+TGG | - | chr4.3:22720024-22720043 | MS.gene052564:CDS | 40.0% |
| ! | GAATGTAAAGGGGATTTGGT+TGG | - | chr4.3:22720243-22720262 | MS.gene052564:CDS | 40.0% |
| ! | GAGGATGAAGATTGGTGATT+TGG | - | chr4.3:22719849-22719868 | MS.gene052564:CDS | 40.0% |
| ! | GCTTTTGATGCAGAGTGATT+TGG | - | chr4.3:22719672-22719691 | MS.gene052564:CDS | 40.0% |
| ! | GTTGTTTCAAAACCGTGTCT+TGG | - | chr4.3:22719781-22719800 | MS.gene052564:CDS | 40.0% |
| ! | TATTGCCTTTGGAGATGAGT+TGG | - | chr4.3:22720413-22720432 | MS.gene052564:CDS | 40.0% |
| ! | TCTTCTTGAGGTTGTTGTTG+AGG | - | chr4.3:22719897-22719916 | MS.gene052564:CDS | 40.0% |
| !! | AAATCTTGTTGGTGTTGAGG+AGG | - | chr4.3:22720287-22720306 | MS.gene052564:CDS | 40.0% |
| !! | AGAACGGGGATTTTGACAAA+TGG | - | chr4.3:22719748-22719767 | MS.gene052564:CDS | 40.0% |
| !! | AGATGAGTTGGTTAGGCAAA+TGG | - | chr4.3:22720425-22720444 | MS.gene052564:CDS | 40.0% |
| !! | AGCTTTGAGGAATCTTCTTG+AGG | - | chr4.3:22719885-22719904 | MS.gene052564:CDS | 40.0% |
| !! | GAACGGGGATTTTGACAAAT+GGG | - | chr4.3:22719749-22719768 | MS.gene052564:CDS | 40.0% |
| !! | GAGAAATCTTGTTGGTGTTG+AGG | - | chr4.3:22720284-22720303 | MS.gene052564:CDS | 40.0% |
| !! | GCGTTGATTTCTCGTATAGT+GGG | - | chr4.3:22719647-22719666 | MS.gene052564:CDS | 40.0% |
| !! | TGCGTTGATTTCTCGTATAG+TGG | - | chr4.3:22719646-22719665 | MS.gene052564:CDS | 40.0% |
| !!! | AAAGGGGTTTTGATTAGTGC+CGG | - | chr4.3:22720060-22720079 | MS.gene052564:CDS | 40.0% |
| !!! | GTTTTTGGGTTCTAATGAGG+TGG | - | chr4.3:22719981-22720000 | MS.gene052564:CDS | 40.0% |
| !!! | TGGGTTTTTGGGTTCTAATG+AGG | - | chr4.3:22719978-22719997 | MS.gene052564:CDS | 40.0% |
| AAGAAAGCAATGGGAGATGC+CGG | - | chr4.3:22720699-22720718 | MS.gene052564:CDS | 45.0% | |
| CAATCCAAAACCCGGATCAA+AGG | + | chr4.3:22720096-22720115 | None:intergenic | 45.0% | |
| CAGGCTTGTGAAATCGAAAG+AGG | - | chr4.3:22720353-22720372 | MS.gene052564:CDS | 45.0% | |
| CATACTGCGGAATCTGTTTG+TGG | + | chr4.3:22721054-22721073 | None:intergenic | 45.0% | |
| CCTAACCAACTCATCTCCAA+AGG | + | chr4.3:22720421-22720440 | None:intergenic | 45.0% | |
| CGGAGAATTCGGATAATGCT+TGG | - | chr4.3:22720166-22720185 | MS.gene052564:CDS | 45.0% | |
| GATCCAGAAGAGGGAAATTC+AGG | - | chr4.3:22720876-22720895 | MS.gene052564:CDS | 45.0% | |
| GCAATTGCTTGATCCAGAAG+AGG | - | chr4.3:22720866-22720885 | MS.gene052564:CDS | 45.0% | |
| GGATAATGCTTGGGCTGTTT+CGG | - | chr4.3:22720176-22720195 | MS.gene052564:CDS | 45.0% | |
| GTGTTATCGCACCTTTGATC+CGG | - | chr4.3:22720082-22720101 | MS.gene052564:CDS | 45.0% | |
| GTTAACCGGAGAATCTCAAC+TGG | - | chr4.3:22719378-22719397 | MS.gene052564:CDS | 45.0% | |
| TGTTATCGCACCTTTGATCC+GGG | - | chr4.3:22720083-22720102 | MS.gene052564:CDS | 45.0% | |
| TTCAATAGTAGCGACGGTGA+AGG | - | chr4.3:22719600-22719619 | MS.gene052564:CDS | 45.0% | |
| TTCTCTTACGCTCTCGATCA+GGG | - | chr4.3:22719462-22719481 | MS.gene052564:CDS | 45.0% | |
| TTGGATTGTGGGAGTGAGTT+GGG | - | chr4.3:22720108-22720127 | MS.gene052564:CDS | 45.0% | |
| TTTCTCTTACGCTCTCGATC+AGG | - | chr4.3:22719461-22719480 | MS.gene052564:CDS | 45.0% | |
| TTTGGATTGTGGGAGTGAGT+TGG | - | chr4.3:22720107-22720126 | MS.gene052564:CDS | 45.0% | |
| ! | ATGCTGTGTCGACGTTTATC+AGG | - | chr4.3:22720334-22720353 | MS.gene052564:CDS | 45.0% |
| ! | ATTTGGTTGGTCCTGCTTGT+GGG | - | chr4.3:22720256-22720275 | MS.gene052564:CDS | 45.0% |
| ! | GATCAAAGGTGCGATAACAC+CGG | + | chr4.3:22720082-22720101 | None:intergenic | 45.0% |
| !! | CCTTTGGAGATGAGTTGGTT+AGG | - | chr4.3:22720418-22720437 | MS.gene052564:CDS | 45.0% |
| !! | GGGGTGTTGAGAAATCTTGT+TGG | - | chr4.3:22720276-22720295 | MS.gene052564:CDS | 45.0% |
| !! | GTGTCTTGGTGCTTGTAAAG+AGG | - | chr4.3:22719795-22719814 | MS.gene052564:CDS | 45.0% |
| !! | TTGGTGTTGAGGAGGTTAAG+AGG | - | chr4.3:22720295-22720314 | MS.gene052564:CDS | 45.0% |
| !!! | AATGGGAGATGCCGGTTTTA+TGG | - | chr4.3:22720707-22720726 | MS.gene052564:CDS | 45.0% |
| !!! | TGATCCGGGTTTTGGATTGT+GGG | - | chr4.3:22720097-22720116 | MS.gene052564:CDS | 45.0% |
| !!! | TTGATCCGGGTTTTGGATTG+TGG | - | chr4.3:22720096-22720115 | MS.gene052564:CDS | 45.0% |
| AAATGAGCTCCAAAGCTCGC+CGG | + | chr4.3:22719432-22719451 | None:intergenic | 50.0% | |
| CACTCCCACAATCCAAAACC+CGG | + | chr4.3:22720104-22720123 | None:intergenic | 50.0% | |
| CTCGCCGGAATCACTATTCT+CGG | + | chr4.3:22719554-22719573 | None:intergenic | 50.0% | |
| GTAGCGTTTGCGAAACTCGA+TGG | - | chr4.3:22719697-22719716 | MS.gene052564:CDS | 50.0% | |
| GTTACCAACCTCGAAAGCGA+CGG | + | chr4.3:22719580-22719599 | None:intergenic | 50.0% | |
| TGGATTGTGGGAGTGAGTTG+GGG | - | chr4.3:22720109-22720128 | MS.gene052564:CDS | 50.0% | |
| TTTCTCAACACCCCACAAGC+AGG | + | chr4.3:22720270-22720289 | None:intergenic | 50.0% | |
| ! | GAGTGAGTTGGGGAAAGTTG+CGG | - | chr4.3:22720119-22720138 | MS.gene052564:CDS | 50.0% |
| ! | GATTTGGTTGGTCCTGCTTG+TGG | - | chr4.3:22720255-22720274 | MS.gene052564:CDS | 50.0% |
| ! | TTTGGTTGGTCCTGCTTGTG+GGG | - | chr4.3:22720257-22720276 | MS.gene052564:CDS | 50.0% |
| !! | GTGAAGGAGTGTCACGATCT+GGG | - | chr4.3:22719616-22719635 | MS.gene052564:CDS | 50.0% |
| ACCGGAGAATCTCAACTGGC+CGG | - | chr4.3:22719382-22719401 | MS.gene052564:CDS | 55.0% | |
| GCCGCCGAGAATAGTGATTC+CGG | - | chr4.3:22719547-22719566 | MS.gene052564:CDS | 55.0% | |
| GCCGGAATCACTATTCTCGG+CGG | + | chr4.3:22719551-22719570 | None:intergenic | 55.0% | |
| GGCACTCTCTGGTATGGTCT+TGG | - | chr4.3:22720788-22720807 | MS.gene052564:CDS | 55.0% | |
| TCAGGGTCTTCGCCGGAAAA+TGG | - | chr4.3:22719479-22719498 | MS.gene052564:CDS | 55.0% | |
| TCCGGCCAGTTGAGATTCTC+CGG | + | chr4.3:22719386-22719405 | None:intergenic | 55.0% | |
| ! | CGCACCTTTGATCCGGGTTT+TGG | - | chr4.3:22720089-22720108 | MS.gene052564:CDS | 55.0% |
| ! | GAATCCGTCGCTTTCGAGGT+TGG | - | chr4.3:22719573-22719592 | MS.gene052564:CDS | 55.0% |
| ! | TTCGGCTCATGGTGGTGTCA+CGG | - | chr4.3:22720194-22720213 | MS.gene052564:CDS | 55.0% |
| !! | GACCATACCAGAGAGTGCCT+CGG | + | chr4.3:22720787-22720806 | None:intergenic | 55.0% |
| !! | GGTGAAGGAGTGTCACGATC+TGG | - | chr4.3:22719615-22719634 | MS.gene052564:CDS | 55.0% |
| AGTTCGCGAAATGGCAGCCG+AGG | - | chr4.3:22720767-22720786 | MS.gene052564:CDS | 60.0% | |
| CTCTCGATCAGGGTCTTCGC+CGG | - | chr4.3:22719472-22719491 | MS.gene052564:CDS | 60.0% | |
| TGGGGAAAGTTGCGGCTGCA+AGG | - | chr4.3:22720127-22720146 | MS.gene052564:CDS | 60.0% | |
| ! | AGCCGAGGCACTCTCTGGTA+TGG | - | chr4.3:22720782-22720801 | MS.gene052564:CDS | 60.0% |
| ! | CGAAAGCGACGGATTCTCGC+CGG | + | chr4.3:22719569-22719588 | None:intergenic | 60.0% |
| ! | GCGAGAATCCGTCGCTTTCG+AGG | - | chr4.3:22719569-22719588 | MS.gene052564:CDS | 60.0% |
| ! | GCTTGGGCTGTTTCGGCTCA+TGG | - | chr4.3:22720183-22720202 | MS.gene052564:CDS | 60.0% |
| ! | TGGGCTGTTTCGGCTCATGG+TGG | - | chr4.3:22720186-22720205 | MS.gene052564:CDS | 60.0% |
| CAACTGGCCGGAGTTCCCAC+CGG | - | chr4.3:22719394-22719413 | MS.gene052564:CDS | 65.0% | |
| GCAAGCTCCGGTGGGAACTC+CGG | + | chr4.3:22719404-22719423 | None:intergenic | 65.0% | |
| GCTCCAAAGCTCGCCGGAGT+CGG | + | chr4.3:22719426-22719445 | None:intergenic | 65.0% | |
| ! | ATGGCAGCCGAGGCACTCTC+TGG | - | chr4.3:22720777-22720796 | MS.gene052564:CDS | 65.0% |
| !! | TTGCCGACTCCGGCGAGCTT+TGG | - | chr4.3:22719420-22719439 | MS.gene052564:CDS | 65.0% |
| CGGAGTCGGCAAGCTCCGGT+GGG | + | chr4.3:22719412-22719431 | None:intergenic | 70.0% | |
| TCGCCGGAGTCGGCAAGCTC+CGG | + | chr4.3:22719416-22719435 | None:intergenic | 70.0% | |
| !! | CCACCGGAGCTTGCCGACTC+CGG | - | chr4.3:22719410-22719429 | MS.gene052564:CDS | 70.0% |
| CCGGAGTCGGCAAGCTCCGG+TGG | + | chr4.3:22719413-22719432 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 22719364 | 22721100 | 22719364 | ID=MS.gene052564 |
| chr4.3 | mRNA | 22719364 | 22721100 | 22719364 | ID=MS.gene052564.t1;Parent=MS.gene052564 |
| chr4.3 | exon | 22719364 | 22721100 | 22719364 | ID=MS.gene052564.t1.exon1;Parent=MS.gene052564.t1 |
| chr4.3 | CDS | 22719364 | 22721100 | 22719364 | ID=cds.MS.gene052564.t1;Parent=MS.gene052564.t1 |
| Gene Sequence |
| Protein sequence |
