Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59910.t1 | XP_003609478.1 | 95 | 201 | 10 | 0 | 1 | 201 | 1 | 201 | 3.80E-104 | 387.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59910.t1 | Q9SIY3 | 55.7 | 210 | 84 | 1 | 1 | 201 | 1 | 210 | 4.9e-60 | 232.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59910.t1 | G7JGL5 | 95.0 | 201 | 10 | 0 | 1 | 201 | 1 | 201 | 2.7e-104 | 387.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene59910 | 0.805707 | 1.17E-49 | -1.69E-46 |
| MS.gene049821 | MS.gene59910 | 0.847626 | 1.03E-59 | -1.69E-46 |
| MS.gene049824 | MS.gene59910 | 0.805807 | 1.12E-49 | -1.69E-46 |
| MS.gene049825 | MS.gene59910 | 0.806644 | 7.44E-50 | -1.69E-46 |
| MS.gene049873 | MS.gene59910 | 0.807002 | 6.25E-50 | -1.69E-46 |
| MS.gene050303 | MS.gene59910 | 0.811543 | 6.64E-51 | -1.69E-46 |
| MS.gene051328 | MS.gene59910 | 0.837318 | 5.58E-57 | -1.69E-46 |
| MS.gene051700 | MS.gene59910 | 0.817478 | 3.23E-52 | -1.69E-46 |
| MS.gene052133 | MS.gene59910 | 0.838289 | 3.14E-57 | -1.69E-46 |
| MS.gene052947 | MS.gene59910 | 0.854506 | 1.18E-61 | -1.69E-46 |
| MS.gene053122 | MS.gene59910 | 0.837151 | 6.16E-57 | -1.69E-46 |
| MS.gene053226 | MS.gene59910 | 0.813249 | 2.82E-51 | -1.69E-46 |
| MS.gene055466 | MS.gene59910 | 0.804277 | 2.33E-49 | -1.69E-46 |
| MS.gene055468 | MS.gene59910 | 0.810033 | 1.41E-50 | -1.69E-46 |
| MS.gene055555 | MS.gene59910 | 0.813556 | 2.41E-51 | -1.69E-46 |
| MS.gene055580 | MS.gene59910 | -0.808028 | 3.79E-50 | -1.69E-46 |
| MS.gene055808 | MS.gene59910 | 0.850439 | 1.70E-60 | -1.69E-46 |
| MS.gene056043 | MS.gene59910 | -0.803939 | 2.74E-49 | -1.69E-46 |
| MS.gene056091 | MS.gene59910 | 0.821589 | 3.73E-53 | -1.69E-46 |
| MS.gene056215 | MS.gene59910 | 0.827683 | 1.37E-54 | -1.69E-46 |
| MS.gene056631 | MS.gene59910 | 0.836613 | 8.44E-57 | -1.69E-46 |
| MS.gene057705 | MS.gene59910 | 0.884502 | 1.82E-71 | -1.69E-46 |
| MS.gene05953 | MS.gene59910 | 0.833738 | 4.49E-56 | -1.69E-46 |
| MS.gene05954 | MS.gene59910 | 0.831782 | 1.37E-55 | -1.69E-46 |
| MS.gene06001 | MS.gene59910 | 0.844946 | 5.52E-59 | -1.69E-46 |
| MS.gene060429 | MS.gene59910 | 0.87769 | 5.13E-69 | -1.69E-46 |
| MS.gene060917 | MS.gene59910 | 0.811464 | 6.91E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59910.t1 | MTR_4g116130 | 95.025 | 201 | 10 | 0 | 1 | 201 | 1 | 201 | 4.43e-138 | 384 |
| MS.gene59910.t1 | MTR_4g116140 | 85.149 | 202 | 29 | 1 | 1 | 201 | 1 | 202 | 1.01e-122 | 345 |
| MS.gene59910.t1 | MTR_0182s0050 | 84.239 | 184 | 29 | 0 | 18 | 201 | 5 | 188 | 3.31e-110 | 313 |
| MS.gene59910.t1 | MTR_1g115240 | 71.144 | 201 | 30 | 1 | 1 | 201 | 1 | 173 | 2.76e-96 | 277 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59910.t1 | AT2G11890 | 55.714 | 210 | 84 | 1 | 1 | 201 | 1 | 210 | 3.03e-66 | 202 |
| MS.gene59910.t1 | AT2G11890 | 47.143 | 210 | 65 | 2 | 1 | 201 | 1 | 173 | 3.51e-47 | 153 |
Find 57 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGGGGTGGTGGGTGAAAA+TGG | 0.155699 | 8.3:-10273909 | MS.gene59910:CDS |
| TGAAAATGGGTTTGTGGGTT+TGG | 0.196016 | 8.3:-10273895 | MS.gene59910:CDS |
| GGGAGGGTGAAGGATGAATT+TGG | 0.200304 | 8.3:-10273927 | MS.gene59910:CDS |
| GTTGGTCGAGTCAAGGATTA+TGG | 0.236549 | 8.3:-10273949 | MS.gene59910:CDS |
| GAAAATGGGTTTGTGGGTTT+GGG | 0.245959 | 8.3:-10273894 | MS.gene59910:CDS |
| TGAAGAAGATGAGGAGGATT+TGG | 0.275997 | 8.3:-10274018 | MS.gene59910:CDS |
| TCGGGATTGCGTTGCTGAAC+CGG | 0.298448 | 8.3:-10273982 | MS.gene59910:CDS |
| GTTGAAAGCAAAGGCGGTTT+TGG | 0.306375 | 8.3:-10274060 | MS.gene59910:CDS |
| TGATTGGAAAGGTTTGAAAT+TGG | 0.318001 | 8.3:-10273844 | MS.gene59910:CDS |
| GTTGCTGAACCGGGGAAGTT+GGG | 0.338043 | 8.3:-10273972 | MS.gene59910:CDS |
| GAAGGATGAATTTGGGGTGG+TGG | 0.339048 | 8.3:-10273919 | MS.gene59910:CDS |
| GGTGGGTGAAAATGGGTTTG+TGG | 0.340645 | 8.3:-10273901 | MS.gene59910:CDS |
| GGAGGGTGAAGGATGAATTT+GGG | 0.368591 | 8.3:-10273926 | MS.gene59910:CDS |
| TTGGGGTGGTGGGTGAAAAT+GGG | 0.381102 | 8.3:-10273908 | MS.gene59910:CDS |
| GTGGGTGAAAATGGGTTTGT+GGG | 0.382924 | 8.3:-10273900 | MS.gene59910:CDS |
| AATGGGTTTGTGGGTTTGGG+AGG | 0.407882 | 8.3:-10273891 | MS.gene59910:CDS |
| CGTTGCTGAACCGGGGAAGT+TGG | 0.412112 | 8.3:-10273973 | MS.gene59910:CDS |
| GAGGATTTGGATCCGAAGAT+TGG | 0.424344 | 8.3:-10274005 | MS.gene59910:CDS |
| AATCTCTTCTTCGACGGCGC+TGG | 0.426538 | 8.3:-10274155 | MS.gene59910:CDS |
| CGGGATTGCGTTGCTGAACC+GGG | 0.433058 | 8.3:-10273981 | MS.gene59910:CDS |
| TTGGTCGAGTCAAGGATTAT+GGG | 0.449548 | 8.3:-10273948 | MS.gene59910:CDS |
| AAGGATGAATTTGGGGTGGT+GGG | 0.462409 | 8.3:-10273918 | MS.gene59910:CDS |
| CTTCTTCGACGGCGCTGGAT+CGG | 0.466202 | 8.3:-10274150 | MS.gene59910:CDS |
| ATGTGAGGAATGTTTATGAT+TGG | 0.470420 | 8.3:-10273860 | MS.gene59910:CDS |
| ACGCAATCCCGACCAATCTT+CGG | 0.484948 | 8.3:+10273993 | None:intergenic |
| AAGGATTATGGGGAGGGTGA+AGG | 0.488009 | 8.3:-10273937 | MS.gene59910:CDS |
| GAGGAGTTCTTGAAGGAGAA+TGG | 0.490553 | 8.3:-10273735 | MS.gene59910:CDS |
| TATTCTCGAGGAGTTCTTGA+AGG | 0.490607 | 8.3:-10273742 | MS.gene59910:CDS |
| GTTCATCTTCACCGTAGAAT+CGG | 0.511621 | 8.3:+10274096 | None:intergenic |
| GTTCTTCGTCTCCGATTCTA+CGG | 0.518461 | 8.3:-10274107 | MS.gene59910:CDS |
| TGAACCGGGGAAGTTGGGGT+TGG | 0.522977 | 8.3:-10273967 | MS.gene59910:CDS |
| GAGGGTGAAGGATGAATTTG+GGG | 0.527844 | 8.3:-10273925 | MS.gene59910:CDS |
| GGTGAAGGATGAATTTGGGG+TGG | 0.535313 | 8.3:-10273922 | MS.gene59910:CDS |
| AGTTGGGGTTGGTCGAGTCA+AGG | 0.540513 | 8.3:-10273956 | MS.gene59910:CDS |
| TCGACCAACCCCAACTTCCC+CGG | 0.541988 | 8.3:+10273963 | None:intergenic |
| ATTTGGATCCGAAGATTGGT+CGG | 0.545088 | 8.3:-10274001 | MS.gene59910:CDS |
| TTGCTGAACCGGGGAAGTTG+GGG | 0.545727 | 8.3:-10273971 | MS.gene59910:CDS |
| TTTGGATCCGAAGATTGGTC+GGG | 0.546401 | 8.3:-10274000 | MS.gene59910:CDS |
| TCGTGTTGAAGAAGATGAGG+AGG | 0.557685 | 8.3:-10274024 | MS.gene59910:CDS |
| TGTTGTTTCGTTGAAAGCAA+AGG | 0.565910 | 8.3:-10274069 | MS.gene59910:CDS |
| TTTGTTTGAGATTGAATGTG+AGG | 0.585638 | 8.3:-10273790 | MS.gene59910:CDS |
| CGAGTCAAGGATTATGGGGA+GGG | 0.587942 | 8.3:-10273943 | MS.gene59910:CDS |
| AGGAATGTTTATGATTGGAA+AGG | 0.602550 | 8.3:-10273855 | MS.gene59910:CDS |
| CAACATAATCTCTTCTTCGA+CGG | 0.610181 | 8.3:-10274161 | MS.gene59910:CDS |
| CGATGAGTGATGACGTGGAA+AGG | 0.614403 | 8.3:+10274185 | None:intergenic |
| TTGTTTGAGATTGAATGTGA+GGG | 0.624852 | 8.3:-10273789 | MS.gene59910:CDS |
| GATTCTACGGTGAAGATGAA+CGG | 0.637964 | 8.3:-10274094 | MS.gene59910:CDS |
| GAGTCGTGTTGAAGAAGATG+AGG | 0.643846 | 8.3:-10274027 | MS.gene59910:CDS |
| CGTCGAAGAAGAGATTATGT+TGG | 0.645098 | 8.3:+10274162 | None:intergenic |
| TGTTTCGTTGAAAGCAAAGG+CGG | 0.647467 | 8.3:-10274066 | MS.gene59910:CDS |
| TGTGAGGGTTCCGATCCTGA+AGG | 0.648829 | 8.3:-10273774 | MS.gene59910:CDS |
| GGGATTGCGTTGCTGAACCG+GGG | 0.653942 | 8.3:-10273980 | MS.gene59910:CDS |
| AGGAGCTAAAAGTATTCTCG+AGG | 0.663418 | 8.3:-10273754 | MS.gene59910:CDS |
| TCGAGTCAAGGATTATGGGG+AGG | 0.669429 | 8.3:-10273944 | MS.gene59910:CDS |
| GAGAAAGCAAAGTAGTGACA+CGG | 0.674575 | 8.3:+10274207 | None:intergenic |
| GTTGGCGATGAGTGATGACG+TGG | 0.723488 | 8.3:+10274180 | None:intergenic |
| TGGTCGAGTCAAGGATTATG+GGG | 0.737226 | 8.3:-10273947 | MS.gene59910:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATGAAACTCATTTTGATTT+TGG | - | chr8.3:10274109-10274128 | MS.gene59910:CDS | 25.0% |
| AGGAATGTTTATGATTGGAA+AGG | - | chr8.3:10274070-10274089 | MS.gene59910:CDS | 30.0% | |
| ATGTGAGGAATGTTTATGAT+TGG | - | chr8.3:10274065-10274084 | MS.gene59910:CDS | 30.0% | |
| ! | TGATTGGAAAGGTTTGAAAT+TGG | - | chr8.3:10274081-10274100 | MS.gene59910:CDS | 30.0% |
| ! | TTGTTTGAGATTGAATGTGA+GGG | - | chr8.3:10274136-10274155 | MS.gene59910:CDS | 30.0% |
| ! | TTTGTTTGAGATTGAATGTG+AGG | - | chr8.3:10274135-10274154 | MS.gene59910:CDS | 30.0% |
| CAACATAATCTCTTCTTCGA+CGG | - | chr8.3:10273764-10273783 | MS.gene59910:CDS | 35.0% | |
| !! | TGTTGTTTCGTTGAAAGCAA+AGG | - | chr8.3:10273856-10273875 | MS.gene59910:CDS | 35.0% |
| !!! | AAATTCGCAATTTTTCGAGC+TGG | - | chr8.3:10274241-10274260 | MS.gene59910:CDS | 35.0% |
| !!! | AATTCGCAATTTTTCGAGCT+GGG | - | chr8.3:10274242-10274261 | MS.gene59910:CDS | 35.0% |
| !!! | AGAATACTTTTAGCTCCTTC+AGG | + | chr8.3:10274169-10274188 | None:intergenic | 35.0% |
| ATTTGGATCCGAAGATTGGT+CGG | - | chr8.3:10273924-10273943 | MS.gene59910:CDS | 40.0% | |
| CGTCGAAGAAGAGATTATGT+TGG | + | chr8.3:10273766-10273785 | None:intergenic | 40.0% | |
| GAGAAAGCAAAGTAGTGACA+CGG | + | chr8.3:10273721-10273740 | None:intergenic | 40.0% | |
| GATTCTACGGTGAAGATGAA+CGG | - | chr8.3:10273831-10273850 | MS.gene59910:CDS | 40.0% | |
| GTTCATCTTCACCGTAGAAT+CGG | + | chr8.3:10273832-10273851 | None:intergenic | 40.0% | |
| TATTCTCGAGGAGTTCTTGA+AGG | - | chr8.3:10274183-10274202 | MS.gene59910:CDS | 40.0% | |
| TGAAGAAGATGAGGAGGATT+TGG | - | chr8.3:10273907-10273926 | MS.gene59910:CDS | 40.0% | |
| TGTTTCGTTGAAAGCAAAGG+CGG | - | chr8.3:10273859-10273878 | MS.gene59910:CDS | 40.0% | |
| TTGGTCGAGTCAAGGATTAT+GGG | - | chr8.3:10273977-10273996 | MS.gene59910:CDS | 40.0% | |
| ! | GAAAATGGGTTTGTGGGTTT+GGG | - | chr8.3:10274031-10274050 | MS.gene59910:CDS | 40.0% |
| ! | TGAAAATGGGTTTGTGGGTT+TGG | - | chr8.3:10274030-10274049 | MS.gene59910:CDS | 40.0% |
| !! | AGGAGCTAAAAGTATTCTCG+AGG | - | chr8.3:10274171-10274190 | MS.gene59910:CDS | 40.0% |
| !!! | ACTTTTAGCTCCTTCAGGAT+CGG | + | chr8.3:10274164-10274183 | None:intergenic | 40.0% |
| !!! | TGGGAGGTTTTAAGAATGTG+AGG | - | chr8.3:10274050-10274069 | MS.gene59910:CDS | 40.0% |
| GAGGAGTTCTTGAAGGAGAA+TGG | - | chr8.3:10274190-10274209 | MS.gene59910:CDS | 45.0% | |
| GAGGATTTGGATCCGAAGAT+TGG | - | chr8.3:10273920-10273939 | MS.gene59910:CDS | 45.0% | |
| GAGGGTGAAGGATGAATTTG+GGG | - | chr8.3:10274000-10274019 | MS.gene59910:CDS | 45.0% | |
| GAGTCGTGTTGAAGAAGATG+AGG | - | chr8.3:10273898-10273917 | MS.gene59910:CDS | 45.0% | |
| GGAGGGTGAAGGATGAATTT+GGG | - | chr8.3:10273999-10274018 | MS.gene59910:CDS | 45.0% | |
| GTTCTTCGTCTCCGATTCTA+CGG | - | chr8.3:10273818-10273837 | MS.gene59910:CDS | 45.0% | |
| GTTGGTCGAGTCAAGGATTA+TGG | - | chr8.3:10273976-10273995 | MS.gene59910:CDS | 45.0% | |
| TCGTGTTGAAGAAGATGAGG+AGG | - | chr8.3:10273901-10273920 | MS.gene59910:CDS | 45.0% | |
| TGGTCGAGTCAAGGATTATG+GGG | - | chr8.3:10273978-10273997 | MS.gene59910:CDS | 45.0% | |
| ! | AAGGATGAATTTGGGGTGGT+GGG | - | chr8.3:10274007-10274026 | MS.gene59910:CDS | 45.0% |
| ! | GTGGGTGAAAATGGGTTTGT+GGG | - | chr8.3:10274025-10274044 | MS.gene59910:CDS | 45.0% |
| ! | GTTGAAAGCAAAGGCGGTTT+TGG | - | chr8.3:10273865-10273884 | MS.gene59910:CDS | 45.0% |
| ! | TTTGGATCCGAAGATTGGTC+GGG | - | chr8.3:10273925-10273944 | MS.gene59910:CDS | 45.0% |
| AAGGATTATGGGGAGGGTGA+AGG | - | chr8.3:10273988-10274007 | MS.gene59910:CDS | 50.0% | |
| ACGCAATCCCGACCAATCTT+CGG | + | chr8.3:10273935-10273954 | None:intergenic | 50.0% | |
| CGAGTCAAGGATTATGGGGA+GGG | - | chr8.3:10273982-10274001 | MS.gene59910:CDS | 50.0% | |
| GGGAGGGTGAAGGATGAATT+TGG | - | chr8.3:10273998-10274017 | MS.gene59910:CDS | 50.0% | |
| GGTGAAGGATGAATTTGGGG+TGG | - | chr8.3:10274003-10274022 | MS.gene59910:CDS | 50.0% | |
| TCGAGTCAAGGATTATGGGG+AGG | - | chr8.3:10273981-10274000 | MS.gene59910:CDS | 50.0% | |
| ! | AATGGGTTTGTGGGTTTGGG+AGG | - | chr8.3:10274034-10274053 | MS.gene59910:CDS | 50.0% |
| ! | CGATGAGTGATGACGTGGAA+AGG | + | chr8.3:10273743-10273762 | None:intergenic | 50.0% |
| ! | GAAGGATGAATTTGGGGTGG+TGG | - | chr8.3:10274006-10274025 | MS.gene59910:CDS | 50.0% |
| ! | GGTGGGTGAAAATGGGTTTG+TGG | - | chr8.3:10274024-10274043 | MS.gene59910:CDS | 50.0% |
| !! | TTGGGGTGGTGGGTGAAAAT+GGG | - | chr8.3:10274017-10274036 | MS.gene59910:CDS | 50.0% |
| !! | TTTGGGGTGGTGGGTGAAAA+TGG | - | chr8.3:10274016-10274035 | MS.gene59910:CDS | 50.0% |
| !!! | GCAAAGGCGGTTTTGGTTGA+TGG | - | chr8.3:10273872-10273891 | MS.gene59910:CDS | 50.0% |
| AATCTCTTCTTCGACGGCGC+TGG | - | chr8.3:10273770-10273789 | MS.gene59910:CDS | 55.0% | |
| GTTGCTGAACCGGGGAAGTT+GGG | - | chr8.3:10273953-10273972 | MS.gene59910:CDS | 55.0% | |
| TCGGGATTGCGTTGCTGAAC+CGG | - | chr8.3:10273943-10273962 | MS.gene59910:CDS | 55.0% | |
| TGTGAGGGTTCCGATCCTGA+AGG | - | chr8.3:10274151-10274170 | MS.gene59910:CDS | 55.0% | |
| TTGCTGAACCGGGGAAGTTG+GGG | - | chr8.3:10273954-10273973 | MS.gene59910:CDS | 55.0% | |
| ! | GTTGGCGATGAGTGATGACG+TGG | + | chr8.3:10273748-10273767 | None:intergenic | 55.0% |
| !! | AGTTGGGGTTGGTCGAGTCA+AGG | - | chr8.3:10273969-10273988 | MS.gene59910:CDS | 55.0% |
| CGGGATTGCGTTGCTGAACC+GGG | - | chr8.3:10273944-10273963 | MS.gene59910:CDS | 60.0% | |
| CGTTGCTGAACCGGGGAAGT+TGG | - | chr8.3:10273952-10273971 | MS.gene59910:CDS | 60.0% | |
| GGGATTGCGTTGCTGAACCG+GGG | - | chr8.3:10273945-10273964 | MS.gene59910:CDS | 60.0% | |
| TCGACCAACCCCAACTTCCC+CGG | + | chr8.3:10273965-10273984 | None:intergenic | 60.0% | |
| TGAACCGGGGAAGTTGGGGT+TGG | - | chr8.3:10273958-10273977 | MS.gene59910:CDS | 60.0% | |
| ! | CTTCTTCGACGGCGCTGGAT+CGG | - | chr8.3:10273775-10273794 | MS.gene59910:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 10273671 | 10274276 | 10273671 | ID=MS.gene59910 |
| chr8.3 | mRNA | 10273671 | 10274276 | 10273671 | ID=MS.gene59910.t1;Parent=MS.gene59910 |
| chr8.3 | exon | 10273671 | 10274276 | 10273671 | ID=MS.gene59910.t1.exon1;Parent=MS.gene59910.t1 |
| chr8.3 | CDS | 10273671 | 10274276 | 10273671 | ID=cds.MS.gene59910.t1;Parent=MS.gene59910.t1 |
| Gene Sequence |
| Protein sequence |
