AlfalfaGEDB | MS.gene61184

M. sativa cultivar XinJiangDaYe / MS.gene61184

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61184.t1	XP_013465831.1	97	263	7	1	1	263	1	262	6.00E-134	486.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61184.t1	Q39262	49.4	172	66	7	51	222	18	168	2.6e-29	130.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61184.t1	A0A072VDM2	97.0	263	7	1	1	263	1	262	4.3e-134	486.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04918	MS.gene61184	0.821678	3.55E-53	-1.69E-46
MS.gene04919	MS.gene61184	0.825165	5.44E-54	-1.69E-46
MS.gene04920	MS.gene61184	0.844229	8.60E-59	-1.69E-46
MS.gene049283	MS.gene61184	0.812932	3.31E-51	-1.69E-46
MS.gene049499	MS.gene61184	0.831304	1.80E-55	-1.69E-46
MS.gene051637	MS.gene61184	0.825172	5.42E-54	-1.69E-46
MS.gene052118	MS.gene61184	0.802221	6.22E-49	-1.69E-46
MS.gene052717	MS.gene61184	0.811039	8.55E-51	-1.69E-46
MS.gene053272	MS.gene61184	-0.821272	4.41E-53	-1.69E-46
MS.gene054142	MS.gene61184	0.83975	1.31E-57	-1.69E-46
MS.gene054957	MS.gene61184	0.803412	3.53E-49	-1.69E-46
MS.gene055150	MS.gene61184	0.804341	2.26E-49	-1.69E-46
MS.gene05686	MS.gene61184	0.811821	5.78E-51	-1.69E-46
MS.gene057102	MS.gene61184	0.810256	1.26E-50	-1.69E-46
MS.gene057148	MS.gene61184	-0.813645	2.31E-51	-1.69E-46
MS.gene058781	MS.gene61184	0.814389	1.58E-51	-1.69E-46
MS.gene05952	MS.gene61184	0.809919	1.49E-50	-1.69E-46
MS.gene059778	MS.gene61184	0.807721	4.40E-50	-1.69E-46
MS.gene05985	MS.gene61184	-0.862043	6.70E-64	-1.69E-46
MS.gene059901	MS.gene61184	-0.835409	1.71E-56	-1.69E-46
MS.gene060512	MS.gene61184	0.83786	4.05E-57	-1.69E-46
MS.gene060522	MS.gene61184	0.831229	1.88E-55	-1.69E-46
MS.gene060906	MS.gene61184	0.834101	3.64E-56	-1.69E-46
MS.gene060933	MS.gene61184	0.818486	1.91E-52	-1.69E-46
MS.gene060935	MS.gene61184	0.817984	2.48E-52	-1.69E-46
MS.gene060937	MS.gene61184	0.837163	6.11E-57	-1.69E-46
MS.gene061100	MS.gene61184	0.827198	1.79E-54	-1.69E-46
MS.gene061160	MS.gene61184	0.802164	6.39E-49	-1.69E-46
MS.gene061188	MS.gene61184	0.828102	1.08E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61184.t1	MTR_1g013130	96.958	263	7	1	1	263	1	262	0.0	532
MS.gene61184.t1	MTR_3g106230	48.729	236	97	9	23	248	4	225	1.46e-57	183
MS.gene61184.t1	MTR_2g104900	35.563	284	125	13	1	263	1	247	2.08e-31	117
MS.gene61184.t1	MTR_4g006575	37.553	237	96	10	46	263	21	224	8.31e-31	114
MS.gene61184.t1	MTR_4g006600	37.553	237	96	10	46	263	21	224	8.31e-31	114
MS.gene61184.t1	MTR_5g077370	34.975	203	86	6	31	211	2	180	1.20e-25	101
MS.gene61184.t1	MTR_1g033340	38.095	147	70	3	35	181	43	168	2.37e-24	98.2
MS.gene61184.t1	MTR_3g065020	54.545	88	22	2	93	180	85	154	6.88e-24	96.7
MS.gene61184.t1	MTR_1g107055	37.594	133	59	3	53	182	21	132	1.29e-18	81.6
MS.gene61184.t1	MTR_4g128340	61.538	52	20	0	91	142	42	93	4.18e-16	74.3
MS.gene61184.t1	MTR_3g011990	30.645	186	97	6	28	198	20	188	7.99e-16	75.9
MS.gene61184.t1	MTR_3g012010	41.176	102	50	3	85	185	89	181	2.26e-14	72.0
MS.gene61184.t1	MTR_3g011970	33.333	186	98	7	28	198	20	194	2.33e-14	71.6
MS.gene61184.t1	MTR_8g466760	37.963	108	51	3	92	198	96	188	6.65e-14	70.5
MS.gene61184.t1	MTR_5g008280	38.793	116	56	4	82	195	34	136	1.82e-13	69.7
MS.gene61184.t1	MTR_5g008310	40.187	107	45	5	91	191	69	162	2.17e-13	69.3
MS.gene61184.t1	MTR_5g008320	38.889	108	45	5	91	191	69	162	4.02e-13	68.6
MS.gene61184.t1	MTR_5g008390	36.036	111	41	3	86	192	6	90	7.90e-13	63.2
MS.gene61184.t1	MTR_7g083210	78.788	33	7	0	94	126	39	71	8.99e-13	65.5
MS.gene61184.t1	MTR_7g086860	70.270	37	11	0	94	130	30	66	1.21e-12	64.3
MS.gene61184.t1	MTR_7g022980	36.441	118	62	5	83	195	55	164	3.34e-12	65.5
MS.gene61184.t1	MTR_3g009410	40.000	95	41	4	92	182	96	178	1.58e-11	63.5
MS.gene61184.t1	MTR_7g026977	32.184	174	102	8	27	198	86	245	2.39e-11	63.5
MS.gene61184.t1	MTR_2g067740	35.238	105	53	2	92	195	57	147	4.28e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61184.t1	AT5G10970	42.616	237	90	9	49	263	60	272	4.04e-44	150
MS.gene61184.t1	AT5G25160	49.419	172	66	7	51	222	18	168	2.83e-39	137
MS.gene61184.t1	AT1G80730	37.395	238	127	7	35	263	4	228	1.90e-32	119
MS.gene61184.t1	AT1G66140	49.515	103	35	3	91	183	80	175	5.67e-22	92.0
MS.gene61184.t1	AT1G24625	42.727	110	37	4	74	179	33	120	1.23e-16	76.6
MS.gene61184.t1	AT5G14010	42.697	89	39	3	94	178	36	116	3.81e-14	68.9
MS.gene61184.t1	AT5G57520	77.778	36	8	0	94	129	50	85	5.01e-14	68.2
MS.gene61184.t1	AT1G67030	38.938	113	55	3	70	180	13	113	3.34e-13	67.0
MS.gene61184.t1	AT3G58070	31.638	177	92	7	23	190	23	179	6.38e-12	64.3
MS.gene61184.t1	AT5G01860	56.250	48	18	1	80	127	54	98	7.33e-11	60.8

Find 32 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCAAACTCAAGAATCTTTA+TGG	0.122503	1.1:+8071097	MS.gene61184:CDS
ATGGCTTCTTTGCCTCTTTA+TGG	0.168485	1.1:+8070957	MS.gene61184:CDS
CTTTGTTTATTATGCATAAT+AGG	0.201649	1.1:-8070924	None:intergenic
CCTTCTCATGTTTCATCTAA+TGG	0.202335	1.1:+8071035	MS.gene61184:CDS
CATGTTTCATCTAATGGATT+TGG	0.228057	1.1:+8071041	MS.gene61184:CDS
AATGGATTTGGAAACTCTTA+TGG	0.229174	1.1:+8071053	MS.gene61184:CDS
ATAGCTTCAGCTTCTGCTTT+TGG	0.233602	1.1:+8070897	MS.gene61184:CDS
TTGCCTCTTTATGGTGCTTT+TGG	0.261823	1.1:+8070966	MS.gene61184:CDS
ATGAAACATGAGAAGGCTTA+TGG	0.351379	1.1:-8071028	None:intergenic
CTCATAACTTCTTTAGATGT+TGG	0.385754	1.1:+8070705	MS.gene61184:CDS
TGCAAAGGTGTGCTTGGACT+TGG	0.428789	1.1:-8070543	None:intergenic
TTCTATAGCTCACAAGCATT+AGG	0.451109	1.1:+8070804	MS.gene61184:CDS
TTTGGTGGTAATAAACCTCT+TGG	0.453972	1.1:+8070984	MS.gene61184:CDS
AAAGGTGTGCTTGGACTTGG+AGG	0.516654	1.1:-8070540	None:intergenic
TGGAATAGCAAAACTAGGTA+TGG	0.517898	1.1:+8071130	MS.gene61184:CDS
ATCTTTATGGATCAACAACC+TGG	0.518646	1.1:+8071110	MS.gene61184:CDS
CAACCTGGAATAGCAAAACT+AGG	0.529841	1.1:+8071125	MS.gene61184:CDS
CCACCAAAAGCACCATAAAG+AGG	0.536698	1.1:-8070969	None:intergenic
CATCAAGAGGAAGTGTTGGT+AGG	0.548091	1.1:+8071177	MS.gene61184:CDS
GAAGAAACAACAATCTATTG+AGG	0.558110	1.1:+8070581	MS.gene61184:CDS
AAACAATAATACTCTTGATG+TGG	0.560949	1.1:+8070743	MS.gene61184:CDS
ATTAATGGAAAGACTAAGCA+AGG	0.563627	1.1:+8070488	None:intergenic
TATGGACATCATCATCATCA+TGG	0.564611	1.1:+8071071	MS.gene61184:CDS
TCTTGAGAAGAGAAGATGCA+TGG	0.579539	1.1:-8070513	None:intergenic
GGTAGAAGAAGATATCACTA+AGG	0.583712	1.1:+8070602	MS.gene61184:CDS
ACATCATCAAGAGGAAGTGT+TGG	0.585725	1.1:+8071173	MS.gene61184:CDS
TATAGCTCACAAGCATTAGG+TGG	0.606344	1.1:+8070807	MS.gene61184:CDS
AGAACAACAACATCATCAAG+AGG	0.611847	1.1:+8071164	MS.gene61184:CDS
ATATTCATAATCATAGCTTG+AGG	0.612882	1.1:-8071271	None:intergenic
TAAACAAAGTTATGCAAACA+TGG	0.616969	1.1:+8070938	MS.gene61184:CDS
CCATTAGATGAAACATGAGA+AGG	0.626125	1.1:-8071035	None:intergenic
GAGTGTGCTTGAATTCCAAG+AGG	0.647089	1.1:-8070999	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CTTTGTTTATTATGCATAAT+AGG	-	chr1.1:8070927-8070946	None:intergenic	20.0%
!	AAACAATAATACTCTTGATG+TGG	+	chr1.1:8070743-8070762	MS.gene61184:CDS	25.0%
!	TAAACAAAGTTATGCAAACA+TGG	+	chr1.1:8070938-8070957	MS.gene61184:CDS	25.0%
!!!	TTTTCATGTTGATTTTGCAA+AGG	-	chr1.1:8070561-8070580	None:intergenic	25.0%
	CTCATAACTTCTTTAGATGT+TGG	+	chr1.1:8070705-8070724	MS.gene61184:CDS	30.0%
	GAAGAAACAACAATCTATTG+AGG	+	chr1.1:8070581-8070600	MS.gene61184:CDS	30.0%
	GTCAAACTCAAGAATCTTTA+TGG	+	chr1.1:8071097-8071116	MS.gene61184:CDS	30.0%
!	AATGGATTTGGAAACTCTTA+TGG	+	chr1.1:8071053-8071072	MS.gene61184:CDS	30.0%
!!	CATGTTTCATCTAATGGATT+TGG	+	chr1.1:8071041-8071060	MS.gene61184:CDS	30.0%
	AGAACAACAACATCATCAAG+AGG	+	chr1.1:8071164-8071183	MS.gene61184:CDS	35.0%
	ATCTTTATGGATCAACAACC+TGG	+	chr1.1:8071110-8071129	MS.gene61184:CDS	35.0%
	ATGAAACATGAGAAGGCTTA+TGG	-	chr1.1:8071031-8071050	None:intergenic	35.0%
	CCATTAGATGAAACATGAGA+AGG	-	chr1.1:8071038-8071057	None:intergenic	35.0%
	CCTTCTCATGTTTCATCTAA+TGG	+	chr1.1:8071035-8071054	MS.gene61184:CDS	35.0%
	GAGAGATCAATAGCAAAAAG+AGG	+	chr1.1:8070852-8070871	MS.gene61184:CDS	35.0%
	GCAAAAAGAGGACAAAGATT+TGG	+	chr1.1:8070864-8070883	MS.gene61184:CDS	35.0%
	GGTAGAAGAAGATATCACTA+AGG	+	chr1.1:8070602-8070621	MS.gene61184:CDS	35.0%
	TATGGACATCATCATCATCA+TGG	+	chr1.1:8071071-8071090	MS.gene61184:CDS	35.0%
	TGGAATAGCAAAACTAGGTA+TGG	+	chr1.1:8071130-8071149	MS.gene61184:CDS	35.0%
	TTCTATAGCTCACAAGCATT+AGG	+	chr1.1:8070804-8070823	MS.gene61184:CDS	35.0%
!	TCTCTCTTTTGTGAGCATTT+TGG	-	chr1.1:8070836-8070855	None:intergenic	35.0%
!	TTTGGTGGTAATAAACCTCT+TGG	+	chr1.1:8070984-8071003	MS.gene61184:CDS	35.0%
!!!	ATACCTAGTTTTGCTATTCC+AGG	-	chr1.1:8071131-8071150	None:intergenic	35.0%
	ATGGCTTCTTTGCCTCTTTA+TGG	+	chr1.1:8070957-8070976	MS.gene61184:CDS	40.0%
	CAACCTGGAATAGCAAAACT+AGG	+	chr1.1:8071125-8071144	MS.gene61184:CDS	40.0%
	GACATCATCATCATCATGGT+TGG	+	chr1.1:8071075-8071094	MS.gene61184:CDS	40.0%
	TATAGCTCACAAGCATTAGG+TGG	+	chr1.1:8070807-8070826	MS.gene61184:CDS	40.0%
	TCTTGAGAAGAGAAGATGCA+TGG	-	chr1.1:8070516-8070535	None:intergenic	40.0%
!	ACATCATCAAGAGGAAGTGT+TGG	+	chr1.1:8071173-8071192	MS.gene61184:CDS	40.0%
!	ATAGCTTCAGCTTCTGCTTT+TGG	+	chr1.1:8070897-8070916	MS.gene61184:CDS	40.0%
!	TTGCCTCTTTATGGTGCTTT+TGG	+	chr1.1:8070966-8070985	MS.gene61184:CDS	40.0%
!!	TGATTTTGCAAAGGTGTGCT+TGG	-	chr1.1:8070552-8070571	None:intergenic	40.0%
	GAGTGTGCTTGAATTCCAAG+AGG	-	chr1.1:8071002-8071021	None:intergenic	45.0%
!	CCTCTTTATGGTGCTTTTGG+TGG	+	chr1.1:8070969-8070988	MS.gene61184:CDS	45.0%
!!	CATCAAGAGGAAGTGTTGGT+AGG	+	chr1.1:8071177-8071196	MS.gene61184:CDS	45.0%
!!	CCACCAAAAGCACCATAAAG+AGG	-	chr1.1:8070972-8070991	None:intergenic	45.0%
!	AAAGGTGTGCTTGGACTTGG+AGG	-	chr1.1:8070543-8070562	None:intergenic	50.0%
!	TGCAAAGGTGTGCTTGGACT+TGG	-	chr1.1:8070546-8070565	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	8070492	8071283	8070492	ID=MS.gene61184
chr1.1	mRNA	8070492	8071283	8070492	ID=MS.gene61184.t1;Parent=MS.gene61184
chr1.1	exon	8070492	8071283	8070492	ID=MS.gene61184.t1.exon1;Parent=MS.gene61184.t1
chr1.1	CDS	8070492	8071283	8070492	ID=cds.MS.gene61184.t1;Parent=MS.gene61184.t1

Gene Sequence

>MS.gene61184

ATGGAAAGACTAAGCAAGGAACCATGCATCTTCTCTTCTCAAGAAACTCCTCCAAGTCCAAGCACACCTTTGCAAAATCAACATGAAAAGAAGAAACAACAATCTATTGAGGTAGAAGAAGATATCACTAAGGTAGAAACTTTATTTGATCTAAATGTATCTAGCAATGATTCTAATCTTGATTGTAATAGTCAGCAAGAAACAGAACTCAATCTCATAACTTCTTTAGATGTTGGTAATTCATCATCACAAAACAATAATACTCTTGATGTGGAACAAAGAATTTTTTCTTGTAACTATTGTCATAGAAAATTCTATAGCTCACAAGCATTAGGTGGACACCAAAATGCTCACAAAAGAGAGAGATCAATAGCAAAAAGAGGACAAAGATTTGGAACACAAATAATAGCTTCAGCTTCTGCTTTTGGATTTCCTATTATGCATAATAAACAAAGTTATGCAAACATGGCTTCTTTGCCTCTTTATGGTGCTTTTGGTGGTAATAAACCTCTTGGAATTCAAGCACACTCTATGATCCATAAGCCTTCTCATGTTTCATCTAATGGATTTGGAAACTCTTATGGACATCATCATCATCATGGTTGGTCAAACTCAAGAATCTTTATGGATCAACAACCTGGAATAGCAAAACTAGGTATGGCTGATTTTCATAGAACAACAACATCATCAAGAGGAAGTGTTGGTAGGTTTGAAGTCGTAGATACTATGATTAATTCTGCAGCTAATAAAGAAATGAAGCAGAAGCAGCTTGATTTAAACCTCAAGCTATGA

Protein sequence

>MS.gene61184.t1

MERLSKEPCIFSSQETPPSPSTPLQNQHEKKKQQSIEVEEDITKVETLFDLNVSSNDSNLDCNSQQETELNLITSLDVGNSSSQNNNTLDVEQRIFSCNYCHRKFYSSQALGGHQNAHKRERSIAKRGQRFGTQIIASASAFGFPIMHNKQSYANMASLPLYGAFGGNKPLGIQAHSMIHKPSHVSSNGFGNSYGHHHHHGWSNSRIFMDQQPGIAKLGMADFHRTTTSSRGSVGRFEVVDTMINSAANKEMKQKQLDLNLKL