AlfalfaGEDB | MS.gene060906

M. sativa cultivar XinJiangDaYe / MS.gene060906

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060906.t1	XP_013465831.1	97.3	262	6	1	1	261	1	262	1.00E-133	486.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060906.t1	Q39262	50.0	172	64	8	50	220	18	168	1.7e-28	127.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060906.t1	A0A072VDM2	97.3	262	6	1	1	261	1	262	7.3e-134	486.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene060338	MS.gene060906	0.800604	1.33E-48	-1.69E-46
MS.gene060906	MS.gene062288	0.804614	1.99E-49	-1.69E-46
MS.gene060906	MS.gene064838	-0.820218	7.70E-53	-1.69E-46
MS.gene060906	MS.gene065509	0.812013	5.25E-51	-1.69E-46
MS.gene060906	MS.gene065510	0.832148	1.12E-55	-1.69E-46
MS.gene060906	MS.gene073556	0.818265	2.15E-52	-1.69E-46
MS.gene060906	MS.gene073557	0.802055	6.73E-49	-1.69E-46
MS.gene060906	MS.gene33836	0.804	2.66E-49	-1.69E-46
MS.gene060906	MS.gene35858	0.805299	1.43E-49	-1.69E-46
MS.gene060906	MS.gene38207	0.802414	5.67E-49	-1.69E-46
MS.gene060906	MS.gene38759	0.813536	2.44E-51	-1.69E-46
MS.gene060906	MS.gene39699	0.803232	3.85E-49	-1.69E-46
MS.gene060906	MS.gene39914	-0.807679	4.49E-50	-1.69E-46
MS.gene060906	MS.gene40100	-0.801217	9.99E-49	-1.69E-46
MS.gene060906	MS.gene48017	0.803071	4.15E-49	-1.69E-46
MS.gene060906	MS.gene52766	-0.805707	1.17E-49	-1.69E-46
MS.gene060906	MS.gene61184	0.834101	3.64E-56	-1.69E-46
MS.gene060906	MS.gene61957	0.831591	1.53E-55	-1.69E-46
MS.gene060906	MS.gene67131	0.806658	7.39E-50	-1.69E-46
MS.gene060906	MS.gene68198	0.821022	5.04E-53	-1.69E-46
MS.gene060906	MS.gene68792	0.817277	3.58E-52	-1.69E-46
MS.gene060906	MS.gene71891	-0.816163	6.37E-52	-1.69E-46
MS.gene060906	MS.gene73393	0.80314	4.02E-49	-1.69E-46
MS.gene060906	MS.gene73644	0.812093	5.04E-51	-1.69E-46
MS.gene060906	MS.gene83864	0.820559	6.43E-53	-1.69E-46
MS.gene060906	MS.gene93967	-0.804673	1.93E-49	-1.69E-46
MS.gene060906	MS.gene98636	0.833775	4.39E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060906.t1	MTR_1g013130	97.328	262	6	1	1	261	1	262	0.0	531
MS.gene060906.t1	MTR_3g106230	49.362	235	95	9	23	246	4	225	1.94e-58	186
MS.gene060906.t1	MTR_2g104900	35.689	283	124	13	1	261	1	247	3.92e-33	121
MS.gene060906.t1	MTR_4g006575	38.136	236	95	10	45	261	21	224	2.33e-31	115
MS.gene060906.t1	MTR_4g006600	38.136	236	95	10	45	261	21	224	2.33e-31	115
MS.gene060906.t1	MTR_1g033340	42.308	130	53	2	50	179	61	168	7.42e-26	102
MS.gene060906.t1	MTR_5g077370	35.149	202	85	6	31	209	2	180	1.18e-25	101
MS.gene060906.t1	MTR_3g065020	56.322	87	21	2	92	178	85	154	3.08e-24	97.8
MS.gene060906.t1	MTR_1g107055	39.706	136	51	5	52	180	21	132	2.14e-19	83.6
MS.gene060906.t1	MTR_3g011990	34.595	185	89	8	28	196	20	188	2.36e-17	80.1
MS.gene060906.t1	MTR_3g011970	32.973	185	98	7	28	196	20	194	5.32e-16	76.3
MS.gene060906.t1	MTR_4g128340	59.615	52	21	0	90	141	42	93	7.03e-16	73.6
MS.gene060906.t1	MTR_8g466760	38.318	107	51	3	91	196	96	188	3.80e-15	73.9
MS.gene060906.t1	MTR_3g012010	41.584	101	50	3	84	183	89	181	1.19e-14	72.8
MS.gene060906.t1	MTR_5g008280	39.130	115	56	4	81	193	34	136	9.79e-14	70.5
MS.gene060906.t1	MTR_5g008310	40.566	106	45	5	90	189	69	162	1.16e-13	70.1
MS.gene060906.t1	MTR_5g008320	39.252	107	45	5	90	189	69	162	2.16e-13	69.3
MS.gene060906.t1	MTR_5g008390	36.364	110	41	3	85	190	6	90	4.05e-13	63.9
MS.gene060906.t1	MTR_7g086860	70.270	37	11	0	93	129	30	66	8.95e-13	64.7
MS.gene060906.t1	MTR_7g083210	75.758	33	8	0	93	125	39	71	1.49e-12	65.1
MS.gene060906.t1	MTR_7g026977	32.558	172	102	7	27	196	86	245	3.26e-12	65.9
MS.gene060906.t1	MTR_7g022980	37.288	118	60	6	82	193	55	164	6.58e-12	64.3
MS.gene060906.t1	MTR_3g009410	40.426	94	41	4	91	180	96	178	8.70e-12	64.3
MS.gene060906.t1	MTR_2g067740	35.577	104	53	2	91	193	57	147	3.37e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060906.t1	AT5G10970	40.068	292	114	11	3	261	9	272	1.57e-46	156
MS.gene060906.t1	AT5G25160	50.000	172	64	8	50	220	18	168	2.04e-38	134
MS.gene060906.t1	AT1G80730	42.439	205	95	8	67	261	37	228	7.23e-32	117
MS.gene060906.t1	AT1G66140	50.000	104	32	4	90	181	80	175	8.08e-21	89.0
MS.gene060906.t1	AT1G24625	44.037	109	36	4	73	177	33	120	6.46e-17	77.4
MS.gene060906.t1	AT5G57520	80.556	36	7	0	93	128	50	85	3.27e-14	68.6
MS.gene060906.t1	AT1G67030	38.462	117	55	4	69	183	13	114	6.13e-14	68.9
MS.gene060906.t1	AT5G14010	76.471	34	8	0	93	126	36	69	4.67e-13	65.9
MS.gene060906.t1	AT3G58070	31.818	176	91	7	23	188	23	179	3.93e-12	65.1
MS.gene060906.t1	AT5G01860	58.333	48	17	1	79	126	54	98	4.61e-11	61.2

Find 30 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCAAACTCAAGAATCTTTA+TGG	0.122503	1.2:+8531333	MS.gene060906:CDS
ATGGCTTCTTTGCCTCTTTA+TGG	0.168485	1.2:+8531196	MS.gene060906:CDS
CTTTGTTTATTATGCATAAT+AGG	0.201649	1.2:-8531163	None:intergenic
CCTTCTCATGTTTCATCTAA+TGG	0.202335	1.2:+8531271	MS.gene060906:CDS
TTGCCTCTTTATGGTGCTTT+TGG	0.219908	1.2:+8531205	MS.gene060906:CDS
CATGTTTCATCTAATGGATT+TGG	0.228057	1.2:+8531277	MS.gene060906:CDS
AATGGATTTGGAAACTCTTA+TGG	0.229174	1.2:+8531289	MS.gene060906:CDS
ATAGCTTCAGCTTCTGCTTT+TGG	0.233602	1.2:+8531136	MS.gene060906:CDS
ATGAAACATGAGAAGGCTTA+TGG	0.351379	1.2:-8531264	None:intergenic
CTCATAACTTCTTTAGATGT+TGG	0.385754	1.2:+8530944	MS.gene060906:CDS
TGCAAAGGTGTGCTTGGACT+TGG	0.428789	1.2:-8530785	None:intergenic
TTCTATAGCTCACAAGCATT+AGG	0.451109	1.2:+8531043	MS.gene060906:CDS
TTACCAAAAGCACCATAAAG+AGG	0.498456	1.2:-8531208	None:intergenic
AAAGGTGTGCTTGGACTTGG+AGG	0.516654	1.2:-8530782	None:intergenic
TGGAATAGCAAAACTAGGTA+TGG	0.517898	1.2:+8531366	MS.gene060906:CDS
ATCTTTATGGATCAACAACC+TGG	0.518646	1.2:+8531346	MS.gene060906:CDS
CAACCTGGAATAGCAAAACT+AGG	0.529841	1.2:+8531361	MS.gene060906:CDS
CATCAAGAGGAAGTGTTGGT+AGG	0.548091	1.2:+8531413	MS.gene060906:CDS
GAAGAAACAACAATCTATTG+AGG	0.558110	1.2:+8530823	MS.gene060906:CDS
ATTAATGGAAAGACTAAGCA+AGG	0.563627	1.2:+8530730	None:intergenic
TATGGACATCATCATCATCA+TGG	0.564611	1.2:+8531307	MS.gene060906:CDS
TCTTGAGAAGAGAAGATGCA+TGG	0.579539	1.2:-8530755	None:intergenic
ACATCATCAAGAGGAAGTGT+TGG	0.585725	1.2:+8531409	MS.gene060906:CDS
TATAGCTCACAAGCATTAGG+TGG	0.606344	1.2:+8531046	MS.gene060906:CDS
AGAACAACAACATCATCAAG+AGG	0.611847	1.2:+8531400	MS.gene060906:CDS
ATATTCATAATCATAGCTTG+AGG	0.612882	1.2:-8531507	None:intergenic
TAAACAAAGTTATGCAAACA+TGG	0.616969	1.2:+8531177	MS.gene060906:CDS
CCATTAGATGAAACATGAGA+AGG	0.626125	1.2:-8531271	None:intergenic
TGAGGTAGAAGATATCACTA+AGG	0.642828	1.2:+8530841	MS.gene060906:CDS
GAGTGTGCTTGAATTCCAAG+AGG	0.647089	1.2:-8531235	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CTTTGTTTATTATGCATAAT+AGG	-	chr1.2:8531166-8531185	None:intergenic	20.0%
!	TAAACAAAGTTATGCAAACA+TGG	+	chr1.2:8531177-8531196	MS.gene060906:CDS	25.0%
!!!	TTTTCATGTTGATTTTGCAA+AGG	-	chr1.2:8530803-8530822	None:intergenic	25.0%
	CTCATAACTTCTTTAGATGT+TGG	+	chr1.2:8530944-8530963	MS.gene060906:CDS	30.0%
	GAAGAAACAACAATCTATTG+AGG	+	chr1.2:8530823-8530842	MS.gene060906:CDS	30.0%
	GTCAAACTCAAGAATCTTTA+TGG	+	chr1.2:8531333-8531352	MS.gene060906:CDS	30.0%
!	AATGGATTTGGAAACTCTTA+TGG	+	chr1.2:8531289-8531308	MS.gene060906:CDS	30.0%
!!	CATGTTTCATCTAATGGATT+TGG	+	chr1.2:8531277-8531296	MS.gene060906:CDS	30.0%
	AGAACAACAACATCATCAAG+AGG	+	chr1.2:8531400-8531419	MS.gene060906:CDS	35.0%
	ATCTTTATGGATCAACAACC+TGG	+	chr1.2:8531346-8531365	MS.gene060906:CDS	35.0%
	ATGAAACATGAGAAGGCTTA+TGG	-	chr1.2:8531267-8531286	None:intergenic	35.0%
	CCATTAGATGAAACATGAGA+AGG	-	chr1.2:8531274-8531293	None:intergenic	35.0%
	CCTTCTCATGTTTCATCTAA+TGG	+	chr1.2:8531271-8531290	MS.gene060906:CDS	35.0%
	GAGAGATCAATAGCAAAAAG+AGG	+	chr1.2:8531091-8531110	MS.gene060906:CDS	35.0%
	GCAAAAAGAGGACAAAGATT+TGG	+	chr1.2:8531103-8531122	MS.gene060906:CDS	35.0%
	TATGGACATCATCATCATCA+TGG	+	chr1.2:8531307-8531326	MS.gene060906:CDS	35.0%
	TGAGGTAGAAGATATCACTA+AGG	+	chr1.2:8530841-8530860	MS.gene060906:CDS	35.0%
	TGGAATAGCAAAACTAGGTA+TGG	+	chr1.2:8531366-8531385	MS.gene060906:CDS	35.0%
	TTCTATAGCTCACAAGCATT+AGG	+	chr1.2:8531043-8531062	MS.gene060906:CDS	35.0%
!	TCTCTCTTTTGTGAGCATTT+TGG	-	chr1.2:8531075-8531094	None:intergenic	35.0%
!!	GCTTTTGGTAATAAACCTCT+TGG	+	chr1.2:8531220-8531239	MS.gene060906:CDS	35.0%
!!	TTACCAAAAGCACCATAAAG+AGG	-	chr1.2:8531211-8531230	None:intergenic	35.0%
!!!	ATACCTAGTTTTGCTATTCC+AGG	-	chr1.2:8531367-8531386	None:intergenic	35.0%
	ATGGCTTCTTTGCCTCTTTA+TGG	+	chr1.2:8531196-8531215	MS.gene060906:CDS	40.0%
	CAACCTGGAATAGCAAAACT+AGG	+	chr1.2:8531361-8531380	MS.gene060906:CDS	40.0%
	GACATCATCATCATCATGGT+TGG	+	chr1.2:8531311-8531330	MS.gene060906:CDS	40.0%
	TATAGCTCACAAGCATTAGG+TGG	+	chr1.2:8531046-8531065	MS.gene060906:CDS	40.0%
	TCTTGAGAAGAGAAGATGCA+TGG	-	chr1.2:8530758-8530777	None:intergenic	40.0%
!	ACATCATCAAGAGGAAGTGT+TGG	+	chr1.2:8531409-8531428	MS.gene060906:CDS	40.0%
!	ATAGCTTCAGCTTCTGCTTT+TGG	+	chr1.2:8531136-8531155	MS.gene060906:CDS	40.0%
!	TTGCCTCTTTATGGTGCTTT+TGG	+	chr1.2:8531205-8531224	MS.gene060906:CDS	40.0%
!!	TGATTTTGCAAAGGTGTGCT+TGG	-	chr1.2:8530794-8530813	None:intergenic	40.0%
	GAGTGTGCTTGAATTCCAAG+AGG	-	chr1.2:8531238-8531257	None:intergenic	45.0%
!!	CATCAAGAGGAAGTGTTGGT+AGG	+	chr1.2:8531413-8531432	MS.gene060906:CDS	45.0%
!	AAAGGTGTGCTTGGACTTGG+AGG	-	chr1.2:8530785-8530804	None:intergenic	50.0%
!	TGCAAAGGTGTGCTTGGACT+TGG	-	chr1.2:8530788-8530807	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	8530734	8531519	8530734	ID=MS.gene060906
chr1.2	mRNA	8530734	8531519	8530734	ID=MS.gene060906.t1;Parent=MS.gene060906
chr1.2	exon	8530734	8531519	8530734	ID=MS.gene060906.t1.exon1;Parent=MS.gene060906.t1
chr1.2	CDS	8530734	8531519	8530734	ID=cds.MS.gene060906.t1;Parent=MS.gene060906.t1

Gene Sequence

>MS.gene060906

ATGGAAAGACTAAGCAAGGAACCATGCATCTTCTCTTCTCAAGAAACTCCTCCAAGTCCAAGCACACCTTTGCAAAATCAACATGAAAAGAAGAAACAACAATCTATTGAGGTAGAAGATATCACTAAGGTAGAAACTTTATTTGATCTAAATGTATCTAGCAATGATTCTAATCTTGATTGTAATAGTCAGCAAGAAACAGAACTCAATCTCATAACTTCTTTAGATGTTGGTAATTCATCATCACAAAACAATAATACTCTTGATGTAGAACAAAGAGTTTTTTCTTGTAACTATTGTCATAGAAAATTCTATAGCTCACAAGCATTAGGTGGACACCAAAATGCTCACAAAAGAGAGAGATCAATAGCAAAAAGAGGACAAAGATTTGGAACACAAATAATAGCTTCAGCTTCTGCTTTTGGATTTCCTATTATGCATAATAAACAAAGTTATGCAAACATGGCTTCTTTGCCTCTTTATGGTGCTTTTGGTAATAAACCTCTTGGAATTCAAGCACACTCTATGATCCATAAGCCTTCTCATGTTTCATCTAATGGATTTGGAAACTCTTATGGACATCATCATCATCATGGTTGGTCAAACTCAAGAATCTTTATGGATCAACAACCTGGAATAGCAAAACTAGGTATGGCTGATTTTCATAGAACAACAACATCATCAAGAGGAAGTGTTGGTAGGTTTGAAGTCGTAGATACTATGATTAATTCTGCAGCTAATAAAGAAATGAAGCAGAAGCAGCTTGATTTAAACCTCAAGCTATGA

Protein sequence

>MS.gene060906.t1

MERLSKEPCIFSSQETPPSPSTPLQNQHEKKKQQSIEVEDITKVETLFDLNVSSNDSNLDCNSQQETELNLITSLDVGNSSSQNNNTLDVEQRVFSCNYCHRKFYSSQALGGHQNAHKRERSIAKRGQRFGTQIIASASAFGFPIMHNKQSYANMASLPLYGAFGNKPLGIQAHSMIHKPSHVSSNGFGNSYGHHHHHGWSNSRIFMDQQPGIAKLGMADFHRTTTSSRGSVGRFEVVDTMINSAANKEMKQKQLDLNLKL