Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61251.t1 | XP_002526487.1 | 100 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 1.30E-43 | 185.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61251.t1 | Q9XHE4 | 97.8 | 92 | 2 | 0 | 1 | 92 | 1 | 92 | 5.9e-45 | 181.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61251.t1 | A0A251S2J9 | 100.0 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 9.4e-44 | 185.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050134 | MS.gene61251 | 0.841583 | 4.35E-58 | -1.69E-46 |
| MS.gene050252 | MS.gene61251 | 0.835912 | 1.27E-56 | -1.69E-46 |
| MS.gene050791 | MS.gene61251 | 0.828467 | 8.85E-55 | -1.69E-46 |
| MS.gene052412 | MS.gene61251 | 0.8047 | 1.90E-49 | -1.69E-46 |
| MS.gene052664 | MS.gene61251 | 0.81076 | 9.82E-51 | -1.69E-46 |
| MS.gene053224 | MS.gene61251 | 0.832187 | 1.09E-55 | -1.69E-46 |
| MS.gene054624 | MS.gene61251 | 0.801137 | 1.04E-48 | -1.69E-46 |
| MS.gene057235 | MS.gene61251 | 0.805222 | 1.48E-49 | -1.69E-46 |
| MS.gene057272 | MS.gene61251 | 0.818799 | 1.62E-52 | -1.69E-46 |
| MS.gene057643 | MS.gene61251 | 0.848431 | 6.16E-60 | -1.69E-46 |
| MS.gene058994 | MS.gene61251 | 0.854738 | 1.01E-61 | -1.69E-46 |
| MS.gene059340 | MS.gene61251 | 0.827598 | 1.43E-54 | -1.69E-46 |
| MS.gene060483 | MS.gene61251 | 0.800641 | 1.31E-48 | -1.69E-46 |
| MS.gene060856 | MS.gene61251 | 0.828723 | 7.67E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61251.t1 | MTR_4g099160 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 9.47e-63 | 185 |
| MS.gene61251.t1 | MTR_4g103340 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 9.47e-63 | 185 |
| MS.gene61251.t1 | MTR_8g007485 | 100.000 | 92 | 0 | 0 | 1 | 92 | 1 | 92 | 9.47e-63 | 185 |
| MS.gene61251.t1 | MTR_0199s0040 | 100.000 | 92 | 0 | 0 | 1 | 92 | 82 | 173 | 1.46e-62 | 187 |
| MS.gene61251.t1 | MTR_4g103340 | 100.000 | 71 | 0 | 0 | 1 | 71 | 1 | 71 | 9.22e-47 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61251.t1 | AT3G10950 | 93.478 | 92 | 6 | 0 | 1 | 92 | 1 | 92 | 4.32e-59 | 176 |
| MS.gene61251.t1 | AT3G60245 | 92.308 | 91 | 7 | 0 | 1 | 91 | 1 | 91 | 8.36e-58 | 172 |
Find 23 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAAAGGCTGTAGGAATA+TGG | 0.316747 | 8.2:-23015507 | MS.gene61251:CDS |
| GAGAAAGGCTGTAGGAATAT+GGG | 0.416937 | 8.2:-23015506 | MS.gene61251:CDS |
| GCTGGTATCGTTGGCAAATA+CGG | 0.433650 | 8.2:-23016421 | MS.gene61251:intron |
| ATGATATTAGGTACCCGTTA+TGG | 0.441488 | 8.2:-23015730 | MS.gene61251:intron |
| CTGCAAGTGCCGTGACTGTT+CGG | 0.445396 | 8.2:-23015347 | MS.gene61251:CDS |
| TGACTGTTCGGAGCACCATC+AGG | 0.472923 | 8.2:-23015335 | MS.gene61251:CDS |
| ACGCAAACTAGCACCATAAC+GGG | 0.476774 | 8.2:+23015717 | None:intergenic |
| TTGTTCCCTCAACCTCCTGA+TGG | 0.500191 | 8.2:+23015320 | None:intergenic |
| GGAGCACCATCAGGAGGTTG+AGG | 0.503709 | 8.2:-23015326 | MS.gene61251:CDS |
| TACGCAAACTAGCACCATAA+CGG | 0.535734 | 8.2:+23015716 | None:intergenic |
| AGAAAGGCTGTAGGAATATG+GGG | 0.537082 | 8.2:-23015505 | MS.gene61251:CDS |
| ACTAAGAGAACTAAGAAGGC+TGG | 0.546098 | 8.2:-23016439 | MS.gene61251:CDS |
| GAGCACCATCAGGAGGTTGA+GGG | 0.548544 | 8.2:-23015325 | MS.gene61251:CDS |
| ACTAAGAAGGCTGGTATCGT+TGG | 0.554051 | 8.2:-23016430 | MS.gene61251:CDS |
| GCAGACTAAGAGAACTAAGA+AGG | 0.566891 | 8.2:-23016443 | MS.gene61251:intron |
| GATTGCGGTAAAGTGAAAGC+AGG | 0.587249 | 8.2:-23015475 | MS.gene61251:CDS |
| TGTAGGAATATGGGGATGCA+AGG | 0.599232 | 8.2:-23015497 | MS.gene61251:CDS |
| GATGGTGCTCCGAACAGTCA+CGG | 0.619064 | 8.2:+23015338 | None:intergenic |
| GCAGTATGCTGTGAAGAGAA+AGG | 0.632531 | 8.2:-23015521 | MS.gene61251:intron |
| TGCGGTAAAGTGAAAGCAGG+CGG | 0.647382 | 8.2:-23015472 | MS.gene61251:CDS |
| ATATGGGGATGCAAGGATTG+CGG | 0.650089 | 8.2:-23015490 | MS.gene61251:CDS |
| GCTGTGAAGAGAAAGGCTGT+AGG | 0.663437 | 8.2:-23015514 | MS.gene61251:CDS |
| CTGTTCGGAGCACCATCAGG+AGG | 0.673364 | 8.2:-23015332 | MS.gene61251:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAAATAATATTTTATGCA+AGG | - | chr8.2:23015882-23015901 | MS.gene61251:intron | 10.0% |
| !!! | ATTATATTTTATGTAAAACA+AGG | + | chr8.2:23016113-23016132 | None:intergenic | 10.0% |
| !!! | TGATTTAAATGTTTTAAATA+TGG | - | chr8.2:23015608-23015627 | MS.gene61251:intron | 10.0% |
| !! | TATACATAGATATATGTATA+TGG | - | chr8.2:23015767-23015786 | MS.gene61251:intron | 15.0% |
| !!! | TTTTATTTTTCAAATCTGAA+AGG | - | chr8.2:23016523-23016542 | MS.gene61251:intron | 15.0% |
| !! | ACAAATACAATTCATGAATT+GGG | + | chr8.2:23016079-23016098 | None:intergenic | 20.0% |
| !! | GAAAAATAAAACATTGAGAA+AGG | + | chr8.2:23016514-23016533 | None:intergenic | 20.0% |
| !! | TACAAATACAATTCATGAAT+TGG | + | chr8.2:23016080-23016099 | None:intergenic | 20.0% |
| !! | TTTATGAGAAGTTAATTCAT+TGG | - | chr8.2:23016168-23016187 | MS.gene61251:intron | 20.0% |
| !!! | AAATATGGTTTTGTTTATGA+TGG | - | chr8.2:23015623-23015642 | MS.gene61251:intron | 20.0% |
| !!! | AAATTTTTCTGTGAATTTTG+TGG | - | chr8.2:23016272-23016291 | MS.gene61251:intron | 20.0% |
| !!! | ATCTAAGTATTTAGTTTAGT+TGG | - | chr8.2:23016336-23016355 | MS.gene61251:intron | 20.0% |
| !!! | TGGTTTTATTGTATGATATT+AGG | - | chr8.2:23016188-23016207 | MS.gene61251:intron | 20.0% |
| ! | AAAACATTGAGAAAGGAATA+AGG | + | chr8.2:23016507-23016526 | None:intergenic | 25.0% |
| ! | AAAATAAACCAATCCAAACT+AGG | + | chr8.2:23015836-23015855 | None:intergenic | 25.0% |
| ! | ACACAGAATTTGTAAGATTT+CGG | + | chr8.2:23015540-23015559 | None:intergenic | 25.0% |
| ! | ATGATATACAACATTACACA+AGG | + | chr8.2:23016307-23016326 | None:intergenic | 25.0% |
| ! | GATTTATTTGTAACTCTTCA+AGG | - | chr8.2:23016136-23016155 | MS.gene61251:intron | 25.0% |
| !! | TTGAAAGAGAAGTTTAGAAT+TGG | + | chr8.2:23015353-23015372 | None:intergenic | 25.0% |
| !!! | TTGGTTTATTTTTGAGTTTG+TGG | - | chr8.2:23015844-23015863 | MS.gene61251:intron | 25.0% |
| ACCTAAAATGTAACTACCTA+GGG | + | chr8.2:23016014-23016033 | None:intergenic | 30.0% | |
| ATAATGAGAACAAAGTCATG+AGG | + | chr8.2:23016553-23016572 | None:intergenic | 30.0% | |
| TTACAAATCTGAGTGAACAA+AGG | + | chr8.2:23015396-23015415 | None:intergenic | 30.0% | |
| ! | AGAGAAGTTTAGAATTGGAT+TGG | + | chr8.2:23015348-23015367 | None:intergenic | 30.0% |
| ! | TTTAGAATTGGATTGGATGA+TGG | + | chr8.2:23015341-23015360 | None:intergenic | 30.0% |
| ! | TTTAGGTGGTTTGATGTTTT+GGG | - | chr8.2:23016027-23016046 | MS.gene61251:intron | 30.0% |
| ! | TTTCTGTGAATTTTGTGGAA+AGG | - | chr8.2:23016277-23016296 | MS.gene61251:intron | 30.0% |
| !! | TTTTAGGTGGTTTGATGTTT+TGG | - | chr8.2:23016026-23016045 | MS.gene61251:intron | 30.0% |
| !!! | TAAGCTATTTTTCATAAGCC+AGG | - | chr8.2:23015988-23016007 | MS.gene61251:intron | 30.0% |
| ATGATATTAGGTACCCGTTA+TGG | - | chr8.2:23016200-23016219 | MS.gene61251:intron | 35.0% | |
| CACCTAAAATGTAACTACCT+AGG | + | chr8.2:23016015-23016034 | None:intergenic | 35.0% | |
| GCGTAAGCAGATTAAAAAGA+TGG | - | chr8.2:23016232-23016251 | MS.gene61251:intron | 35.0% | |
| GGTAATTGATCATCAGAACT+CGG | + | chr8.2:23016385-23016404 | None:intergenic | 35.0% | |
| ! | CTAGGTAGTTACATTTTAGG+TGG | - | chr8.2:23016013-23016032 | MS.gene61251:intron | 35.0% |
| ! | TTAGGTGGTTTGATGTTTTG+GGG | - | chr8.2:23016028-23016047 | MS.gene61251:intron | 35.0% |
| ACTAAGAGAACTAAGAAGGC+TGG | - | chr8.2:23015491-23015510 | MS.gene61251:CDS | 40.0% | |
| AGAAAGGCTGTAGGAATATG+GGG | - | chr8.2:23016425-23016444 | MS.gene61251:CDS | 40.0% | |
| AGAGAAAGGCTGTAGGAATA+TGG | - | chr8.2:23016423-23016442 | MS.gene61251:CDS | 40.0% | |
| GAGAAAGGCTGTAGGAATAT+GGG | - | chr8.2:23016424-23016443 | MS.gene61251:CDS | 40.0% | |
| GCAGACTAAGAGAACTAAGA+AGG | - | chr8.2:23015487-23015506 | MS.gene61251:CDS | 40.0% | |
| TACGCAAACTAGCACCATAA+CGG | + | chr8.2:23016217-23016236 | None:intergenic | 40.0% | |
| TGACTACACCTAGTTTGGAT+TGG | - | chr8.2:23015825-23015844 | MS.gene61251:intron | 40.0% | |
| ! | GCCCTAGGTAGTTACATTTT+AGG | - | chr8.2:23016010-23016029 | MS.gene61251:intron | 40.0% |
| !! | ATTGGATTGGATGATGGAGA+AGG | + | chr8.2:23015335-23015354 | None:intergenic | 40.0% |
| !! | CAGAACTCGGCATTTTGAAA+AGG | + | chr8.2:23016372-23016391 | None:intergenic | 40.0% |
| !! | TAGGTGGTTTGATGTTTTGG+GGG | - | chr8.2:23016029-23016048 | MS.gene61251:intron | 40.0% |
| !! | TTGGATTGGATGATGGAGAA+GGG | + | chr8.2:23015334-23015353 | None:intergenic | 40.0% |
| AAATGTAACTACCTAGGGCC+TGG | + | chr8.2:23016009-23016028 | None:intergenic | 45.0% | |
| ACGCAAACTAGCACCATAAC+GGG | + | chr8.2:23016216-23016235 | None:intergenic | 45.0% | |
| ACTAAGAAGGCTGGTATCGT+TGG | - | chr8.2:23015500-23015519 | MS.gene61251:CDS | 45.0% | |
| ATATGGGGATGCAAGGATTG+CGG | - | chr8.2:23016440-23016459 | MS.gene61251:CDS | 45.0% | |
| GATTGCGGTAAAGTGAAAGC+AGG | - | chr8.2:23016455-23016474 | MS.gene61251:intron | 45.0% | |
| GCAGTATGCTGTGAAGAGAA+AGG | - | chr8.2:23016409-23016428 | MS.gene61251:intron | 45.0% | |
| GCTGGTATCGTTGGCAAATA+CGG | - | chr8.2:23015509-23015528 | MS.gene61251:CDS | 45.0% | |
| GTAGCTGACTACACCTAGTT+TGG | - | chr8.2:23015820-23015839 | MS.gene61251:intron | 45.0% | |
| TCTTCACAGCATACTGCAAC+AGG | + | chr8.2:23016406-23016425 | None:intergenic | 45.0% | |
| TGAGAACAAAGTCATGAGGC+TGG | + | chr8.2:23016549-23016568 | None:intergenic | 45.0% | |
| TGTAGGAATATGGGGATGCA+AGG | - | chr8.2:23016433-23016452 | MS.gene61251:CDS | 45.0% | |
| ! | TTTTTCATAAGCCAGGCCCT+AGG | - | chr8.2:23015995-23016014 | MS.gene61251:intron | 45.0% |
| !! | TGGATTGGATGATGGAGAAG+GGG | + | chr8.2:23015333-23015352 | None:intergenic | 45.0% |
| GCTGTGAAGAGAAAGGCTGT+AGG | - | chr8.2:23016416-23016435 | MS.gene61251:intron | 50.0% | |
| TGCGGTAAAGTGAAAGCAGG+CGG | - | chr8.2:23016458-23016477 | MS.gene61251:intron | 50.0% | |
| TTGTTCCCTCAACCTCCTGA+TGG | + | chr8.2:23016613-23016632 | None:intergenic | 50.0% | |
| ! | ATGATGGAGAAGGGGAGAAG+GGG | + | chr8.2:23015325-23015344 | None:intergenic | 50.0% |
| !! | GATGATGGAGAAGGGGAGAA+GGG | + | chr8.2:23015326-23015345 | None:intergenic | 50.0% |
| GAGCACCATCAGGAGGTTGA+GGG | - | chr8.2:23016605-23016624 | MS.gene61251:intron | 55.0% | |
| GATGGTGCTCCGAACAGTCA+CGG | + | chr8.2:23016595-23016614 | None:intergenic | 55.0% | |
| ! | CTGCAAGTGCCGTGACTGTT+CGG | - | chr8.2:23016583-23016602 | MS.gene61251:intron | 55.0% |
| ! | TGACTGTTCGGAGCACCATC+AGG | - | chr8.2:23016595-23016614 | MS.gene61251:intron | 55.0% |
| !! | GGATGATGGAGAAGGGGAGA+AGG | + | chr8.2:23015327-23015346 | None:intergenic | 55.0% |
| ! | CTGTTCGGAGCACCATCAGG+AGG | - | chr8.2:23016598-23016617 | MS.gene61251:intron | 60.0% |
| ! | GGAGCACCATCAGGAGGTTG+AGG | - | chr8.2:23016604-23016623 | MS.gene61251:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 23015308 | 23016644 | 23015308 | ID=MS.gene61251 |
| chr8.2 | mRNA | 23015308 | 23016644 | 23015308 | ID=MS.gene61251.t1;Parent=MS.gene61251 |
| chr8.2 | exon | 23016642 | 23016644 | 23016642 | ID=MS.gene61251.t1.exon1;Parent=MS.gene61251.t1 |
| chr8.2 | CDS | 23016642 | 23016644 | 23016642 | ID=cds.MS.gene61251.t1;Parent=MS.gene61251.t1 |
| chr8.2 | exon | 23016422 | 23016461 | 23016422 | ID=MS.gene61251.t1.exon2;Parent=MS.gene61251.t1 |
| chr8.2 | CDS | 23016422 | 23016461 | 23016422 | ID=cds.MS.gene61251.t1;Parent=MS.gene61251.t1 |
| chr8.2 | exon | 23015654 | 23015742 | 23015654 | ID=MS.gene61251.t1.exon3;Parent=MS.gene61251.t1 |
| chr8.2 | CDS | 23015654 | 23015742 | 23015654 | ID=cds.MS.gene61251.t1;Parent=MS.gene61251.t1 |
| chr8.2 | exon | 23015457 | 23015539 | 23015457 | ID=MS.gene61251.t1.exon4;Parent=MS.gene61251.t1 |
| chr8.2 | CDS | 23015457 | 23015539 | 23015457 | ID=cds.MS.gene61251.t1;Parent=MS.gene61251.t1 |
| chr8.2 | exon | 23015308 | 23015371 | 23015308 | ID=MS.gene61251.t1.exon5;Parent=MS.gene61251.t1 |
| chr8.2 | CDS | 23015308 | 23015371 | 23015308 | ID=cds.MS.gene61251.t1;Parent=MS.gene61251.t1 |
| Gene Sequence |
| Protein sequence |
