AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052412


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052412.t1 MTR_6g085000 97.906 191 4 0 1 191 1 191 2.18e-137 381
MS.gene052412.t1 MTR_6g021670 97.382 191 5 0 1 191 1 191 4.81e-137 380
MS.gene052412.t1 MTR_8g087480 63.636 44 8 1 145 188 51 86 8.01e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052412.t1 AT1G48830 79.058 191 40 0 1 191 1 191 1.48e-114 324
MS.gene052412.t1 AT1G48830 79.058 191 40 0 1 191 1 191 1.48e-114 324
MS.gene052412.t1 AT3G02560 79.581 191 39 0 1 191 1 191 5.50e-113 320
MS.gene052412.t1 AT3G02560 79.581 191 39 0 1 191 1 191 5.50e-113 320
MS.gene052412.t1 AT3G02560 79.581 191 39 0 1 191 1 191 5.50e-113 320
MS.gene052412.t1 AT5G16130 80.319 188 37 0 1 188 1 188 1.92e-112 318

Find 51 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAGCCAAGTTGATGTTTC+TGG 0.145762 6.1:+74576319 MS.gene052412:intron
TCCTCAATCTGTAGGGGATA+TGG 0.261869 6.1:-74576363 None:intergenic
AGAATCTACGCCTCAGTAAC+AGG 0.280462 6.1:-74577439 None:intergenic
TGAGCAGCGGAGCCTTTCTT+TGG 0.288203 6.1:-74576991 None:intergenic
ATAGTTTACCTTCATGATCT+TGG 0.292174 6.1:-74577121 None:intergenic
AAATTCACGTCAAGCTCGTT+AGG 0.363074 6.1:+74576398 MS.gene052412:CDS
CGTACTAGATACAGAATTGA+TGG 0.367485 6.1:+74577096 MS.gene052412:CDS
GTTGCAGGCTTTGTTTGATC+TGG 0.412438 6.1:+74576158 MS.gene052412:intron
CGCTGAGCCTACTGAATTTG+AGG 0.426295 6.1:+74576025 MS.gene052412:CDS
GCAGCGGAGCCTTTCTTTGG+TGG 0.435938 6.1:-74576988 None:intergenic
TTGTGAATATGTTTACTTCA+AGG 0.441187 6.1:+74575979 None:intergenic
GCAGTTTACAGGAAACTCTC+AGG 0.456482 6.1:+74577394 MS.gene052412:CDS
ATTTGAGGAGTCAGTTGGAC+AGG 0.472058 6.1:+74576040 MS.gene052412:CDS
TGTTACCTGCTGAGATTGTT+GGG 0.479284 6.1:+74577070 MS.gene052412:CDS
TGGTGGCCTGACGATTCTCC+TGG 0.483590 6.1:-74576971 None:intergenic
GTGTTACCTGCTGAGATTGT+TGG 0.484291 6.1:+74577069 MS.gene052412:CDS
TCCCCTACAGATTGAGGAAG+GGG 0.499187 6.1:+74576368 MS.gene052412:CDS
CGTGGACAGCAGTGAGTGTA+CGG 0.505350 6.1:-74577026 None:intergenic
ATCGTCAGGCCACCAAAGAA+AGG 0.508890 6.1:+74576979 MS.gene052412:CDS
TTCTGGAAACCGCAAGGCTG+TGG 0.509249 6.1:+74576336 MS.gene052412:CDS
TCCATATCCCCTACAGATTG+AGG 0.509912 6.1:+74576362 MS.gene052412:CDS
TTGCCACCAGGAGAATCGTC+AGG 0.510117 6.1:+74576965 MS.gene052412:CDS
GAAGAAAATCAGCAAGGACA+AGG 0.510615 6.1:+74576001 MS.gene052412:CDS
GAACCCCTTCCTCAATCTGT+AGG 0.515858 6.1:-74576371 None:intergenic
AACCCCTTCCTCAATCTGTA+GGG 0.520673 6.1:-74576370 None:intergenic
ACTGAATTTGAGGAGTCAGT+TGG 0.548538 6.1:+74576035 MS.gene052412:CDS
AATTCACGTCAAGCTCGTTA+GGG 0.550766 6.1:+74576399 MS.gene052412:CDS
GCTGCGTGGACGCTGAGCAG+CGG 0.558388 6.1:-74577004 None:intergenic
CGTTTCCCAACAATCTCAGC+AGG 0.558735 6.1:-74577075 None:intergenic
GTTTGAGTACCCTGTTACTG+AGG 0.572498 6.1:+74577429 MS.gene052412:CDS
TATCCCCTACAGATTGAGGA+AGG 0.573232 6.1:+74576366 MS.gene052412:CDS
GGTTTCCAGAAACATCAACT+TGG 0.575562 6.1:-74576324 None:intergenic
ATGTAATCCTCATTGCCACC+AGG 0.584954 6.1:+74576953 MS.gene052412:CDS
CGATTCTCCTGGTGGCAATG+AGG 0.590532 6.1:-74576960 None:intergenic
AAGAAAATCAGCAAGGACAA+GGG 0.599976 6.1:+74576002 MS.gene052412:CDS
CAGTTTACAGGAAACTCTCA+GGG 0.612531 6.1:+74577395 MS.gene052412:CDS
TGAGAAGAAATTCAGCGGAA+AGG 0.621961 6.1:+74576426 MS.gene052412:CDS
TGATGTTTCTGGAAACCGCA+AGG 0.622049 6.1:+74576330 MS.gene052412:CDS
ATCCCCTACAGATTGAGGAA+GGG 0.622569 6.1:+74576367 MS.gene052412:CDS
ACTGACTCCTCAAATTCAGT+AGG 0.622702 6.1:-74576032 None:intergenic
ACCCCTTCCTCAATCTGTAG+GGG 0.633929 6.1:-74576369 None:intergenic
TGATGGATCCAAGATCATGA+AGG 0.639271 6.1:+74577113 MS.gene052412:CDS
TGTCCACGAGGCAATGCTCG+AGG 0.642990 6.1:+74577041 MS.gene052412:CDS
TTCAAGGAAGAAAATCAGCA+AGG 0.648771 6.1:+74575995 MS.gene052412:CDS
GCAGTGAGTGTACGGCTGCG+TGG 0.657285 6.1:-74577018 None:intergenic
TACACTCACTGCTGTCCACG+AGG 0.659160 6.1:+74577029 MS.gene052412:CDS
TGGCCTGACGATTCTCCTGG+TGG 0.662830 6.1:-74576968 None:intergenic
TATGGATAACCACAGCCTTG+CGG 0.675676 6.1:-74576345 None:intergenic
GAATCTACGCCTCAGTAACA+GGG 0.678065 6.1:-74577438 None:intergenic
CATCCTCGAGCATTGCCTCG+TGG 0.690997 6.1:-74577044 None:intergenic
GAGCTTGAGAAGAAATTCAG+CGG 0.694607 6.1:+74576421 MS.gene052412:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGGAACAAAAAATTGT+CGG - chr6.1:74576523-74576542 None:intergenic 20.0%
!! AGAATAAAGTTCTTGAAATA+AGG - chr6.1:74576572-74576591 None:intergenic 20.0%
!! CAAATTATTTAGTGTATGTT+TGG - chr6.1:74576837-74576856 None:intergenic 20.0%
!! TTAAAGCAATTTAAAAACCA+AGG - chr6.1:74576607-74576626 None:intergenic 20.0%
!!! AGGTTATATATTGATTACTA+CGG + chr6.1:74576060-74576079 MS.gene052412:intron 20.0%
! TATTTAGTGTATGTTTGGTT+TGG - chr6.1:74576832-74576851 None:intergenic 25.0%
!! TGTGAGAAAACAATTTTTCA+AGG - chr6.1:74576878-74576897 None:intergenic 25.0%
!!! AGTAACTTTTGGAAATTGTT+TGG - chr6.1:74577155-74577174 None:intergenic 25.0%
!!! TGCTTATTTTCACATTAGTT+TGG - chr6.1:74577211-74577230 None:intergenic 25.0%
AACAAAAACTCACGATTACA+TGG + chr6.1:74576730-74576749 MS.gene052412:intron 30.0%
ATAGTTTACCTTCATGATCT+TGG - chr6.1:74577124-74577143 None:intergenic 30.0%
CTAATGAACCTTAAACTTGT+AGG + chr6.1:74576930-74576949 MS.gene052412:intron 30.0%
CTGTAAAACTGAAGTTAATC+AGG + chr6.1:74576658-74576677 MS.gene052412:intron 30.0%
GACAGAATCGTAATAAAAGA+TGG - chr6.1:74576498-74576517 None:intergenic 30.0%
GATTACATCCTACAAGTTTA+AGG - chr6.1:74576941-74576960 None:intergenic 30.0%
TTAGAAAGAATAACTGGCAA+AGG - chr6.1:74576470-74576489 None:intergenic 30.0%
! TCTGTGTATATAGGTGTTTT+TGG + chr6.1:74577324-74577343 MS.gene052412:intron 30.0%
! TCTTGTGTTTCTGTGTATAT+AGG + chr6.1:74577315-74577334 MS.gene052412:intron 30.0%
!! CAGTATTGTTTTTCTCCTTT+GGG - chr6.1:74577351-74577370 None:intergenic 30.0%
!! TCAGTATTGTTTTTCTCCTT+TGG - chr6.1:74577352-74577371 None:intergenic 30.0%
!!! TGTTTTCAATTTGTTGTTGC+AGG + chr6.1:74576143-74576162 MS.gene052412:intron 30.0%
!!! TTCAAGCTTTGAGTAACTTT+TGG - chr6.1:74577166-74577185 None:intergenic 30.0%
AAGAAAATCAGCAAGGACAA+GGG + chr6.1:74576002-74576021 MS.gene052412:CDS 35.0%
CGAGTAGAAGACAAAGAAAT+TGG - chr6.1:74576539-74576558 None:intergenic 35.0%
CGTACTAGATACAGAATTGA+TGG + chr6.1:74577096-74577115 MS.gene052412:CDS 35.0%
GATAAAGAGAAGCTACAAAG+TGG - chr6.1:74576761-74576780 None:intergenic 35.0%
GTCTAAGCATGTGTTTGATT+TGG + chr6.1:74576686-74576705 MS.gene052412:intron 35.0%
GTGCAGTTAGAAAGAATAAC+TGG - chr6.1:74576476-74576495 None:intergenic 35.0%
GTGTAAGGATGTGTTCTTTA+TGG + chr6.1:74576237-74576256 MS.gene052412:intron 35.0%
TACATCAATTCTGCAGTGTA+AGG + chr6.1:74576222-74576241 MS.gene052412:intron 35.0%
TGGCTGTAGATAACAGAAAA+CGG - chr6.1:74576307-74576326 None:intergenic 35.0%
TTCAAGGAAGAAAATCAGCA+AGG + chr6.1:74575995-74576014 MS.gene052412:CDS 35.0%
! AAACGTTTTCTGCAGTTTAC+AGG + chr6.1:74577383-74577402 MS.gene052412:CDS 35.0%
! ACTATTGTTGTTGCTGTGAT+TGG + chr6.1:74576083-74576102 MS.gene052412:intron 35.0%
! ATTCTGTTTTGCAATCTCCT+TGG + chr6.1:74576587-74576606 MS.gene052412:intron 35.0%
AAATTCACGTCAAGCTCGTT+AGG + chr6.1:74576398-74576417 MS.gene052412:CDS 40.0%
AACTGAAGTTAATCAGGCTG+CGG + chr6.1:74576664-74576683 MS.gene052412:intron 40.0%
AATTCACGTCAAGCTCGTTA+GGG + chr6.1:74576399-74576418 MS.gene052412:CDS 40.0%
ACTGAATTTGAGGAGTCAGT+TGG + chr6.1:74576035-74576054 MS.gene052412:CDS 40.0%
CAGTTTACAGGAAACTCTCA+GGG + chr6.1:74577395-74577414 MS.gene052412:CDS 40.0%
GAAGAAAATCAGCAAGGACA+AGG + chr6.1:74576001-74576020 MS.gene052412:CDS 40.0%
GAGCTTGAGAAGAAATTCAG+CGG + chr6.1:74576421-74576440 MS.gene052412:CDS 40.0%
GGTTTCCAGAAACATCAACT+TGG - chr6.1:74576327-74576346 None:intergenic 40.0%
TGAGAAGAAATTCAGCGGAA+AGG + chr6.1:74576426-74576445 MS.gene052412:CDS 40.0%
TGTTACCTGCTGAGATTGTT+GGG + chr6.1:74577070-74577089 MS.gene052412:CDS 40.0%
! ACTGACTCCTCAAATTCAGT+AGG - chr6.1:74576035-74576054 None:intergenic 40.0%
! TGATGGATCCAAGATCATGA+AGG + chr6.1:74577113-74577132 MS.gene052412:CDS 40.0%
!! TACAGCCAAGTTGATGTTTC+TGG + chr6.1:74576319-74576338 MS.gene052412:intron 40.0%
!! TCAGATCACTTTTGAGCTCA+TGG - chr6.1:74576194-74576213 None:intergenic 40.0%
!!! ATAGGTGTTTTTGGACCCAA+AGG + chr6.1:74577333-74577352 MS.gene052412:intron 40.0%
AACCCCTTCCTCAATCTGTA+GGG - chr6.1:74576373-74576392 None:intergenic 45.0%
ATCCCCTACAGATTGAGGAA+GGG + chr6.1:74576367-74576386 MS.gene052412:CDS 45.0%
ATGCCTTGTAATCATGCGAG+TGG + chr6.1:74576793-74576812 MS.gene052412:intron 45.0%
ATGTAATCCTCATTGCCACC+AGG + chr6.1:74576953-74576972 MS.gene052412:CDS 45.0%
ATTTGAGGAGTCAGTTGGAC+AGG + chr6.1:74576040-74576059 MS.gene052412:CDS 45.0%
GCAGTTTACAGGAAACTCTC+AGG + chr6.1:74577394-74577413 MS.gene052412:CDS 45.0%
GTGTTACCTGCTGAGATTGT+TGG + chr6.1:74577069-74577088 MS.gene052412:CDS 45.0%
GTTTGAGTACCCTGTTACTG+AGG + chr6.1:74577429-74577448 MS.gene052412:CDS 45.0%
TATCCCCTACAGATTGAGGA+AGG + chr6.1:74576366-74576385 MS.gene052412:CDS 45.0%
TATGGATAACCACAGCCTTG+CGG - chr6.1:74576348-74576367 None:intergenic 45.0%
TCCATATCCCCTACAGATTG+AGG + chr6.1:74576362-74576381 MS.gene052412:CDS 45.0%
TCCTCAATCTGTAGGGGATA+TGG - chr6.1:74576366-74576385 None:intergenic 45.0%
TTGCCACTCGCATGATTACA+AGG - chr6.1:74576799-74576818 None:intergenic 45.0%
! CGAGCTTGACGTGAATTTTG+CGG - chr6.1:74576396-74576415 None:intergenic 45.0%
!! GTTGCAGGCTTTGTTTGATC+TGG + chr6.1:74576158-74576177 MS.gene052412:intron 45.0%
!! TGATGTTTCTGGAAACCGCA+AGG + chr6.1:74576330-74576349 MS.gene052412:CDS 45.0%
ACCCCTTCCTCAATCTGTAG+GGG - chr6.1:74576372-74576391 None:intergenic 50.0%
ATCGTCAGGCCACCAAAGAA+AGG + chr6.1:74576979-74576998 MS.gene052412:CDS 50.0%
CGCTGAGCCTACTGAATTTG+AGG + chr6.1:74576025-74576044 MS.gene052412:CDS 50.0%
CGTTTCCCAACAATCTCAGC+AGG - chr6.1:74577078-74577097 None:intergenic 50.0%
GAACCCCTTCCTCAATCTGT+AGG - chr6.1:74576374-74576393 None:intergenic 50.0%
TCCCCTACAGATTGAGGAAG+GGG + chr6.1:74576368-74576387 MS.gene052412:CDS 50.0%
CGATTCTCCTGGTGGCAATG+AGG - chr6.1:74576963-74576982 None:intergenic 55.0%
CGTGGACAGCAGTGAGTGTA+CGG - chr6.1:74577029-74577048 None:intergenic 55.0%
TACACTCACTGCTGTCCACG+AGG + chr6.1:74577029-74577048 MS.gene052412:CDS 55.0%
TGAGCAGCGGAGCCTTTCTT+TGG - chr6.1:74576994-74577013 None:intergenic 55.0%
TTGCCACCAGGAGAATCGTC+AGG + chr6.1:74576965-74576984 MS.gene052412:CDS 55.0%
! TTCTGGAAACCGCAAGGCTG+TGG + chr6.1:74576336-74576355 MS.gene052412:CDS 55.0%
GCAGCGGAGCCTTTCTTTGG+TGG - chr6.1:74576991-74577010 None:intergenic 60.0%
TGGCCTGACGATTCTCCTGG+TGG - chr6.1:74576971-74576990 None:intergenic 60.0%
TGGTGGCCTGACGATTCTCC+TGG - chr6.1:74576974-74576993 None:intergenic 60.0%
TGTCCACGAGGCAATGCTCG+AGG + chr6.1:74577041-74577060 MS.gene052412:CDS 60.0%
! CATCCTCGAGCATTGCCTCG+TGG - chr6.1:74577047-74577066 None:intergenic 60.0%
GCAGTGAGTGTACGGCTGCG+TGG - chr6.1:74577021-74577040 None:intergenic 65.0%
GCTGCGTGGACGCTGAGCAG+CGG - chr6.1:74577007-74577026 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 74575987 74577456 74575987 ID=MS.gene052412
chr6.1 mRNA 74575987 74577456 74575987 ID=MS.gene052412.t1;Parent=MS.gene052412
chr6.1 exon 74575987 74576061 74575987 ID=MS.gene052412.t1.exon1;Parent=MS.gene052412.t1
chr6.1 CDS 74575987 74576061 74575987 ID=cds.MS.gene052412.t1;Parent=MS.gene052412.t1
chr6.1 exon 74576165 74576238 74576165 ID=MS.gene052412.t1.exon2;Parent=MS.gene052412.t1
chr6.1 CDS 74576165 74576238 74576165 ID=cds.MS.gene052412.t1;Parent=MS.gene052412.t1
chr6.1 exon 74576324 74576447 74576324 ID=MS.gene052412.t1.exon3;Parent=MS.gene052412.t1
chr6.1 CDS 74576324 74576447 74576324 ID=cds.MS.gene052412.t1;Parent=MS.gene052412.t1
chr6.1 exon 74576952 74577134 74576952 ID=MS.gene052412.t1.exon4;Parent=MS.gene052412.t1
chr6.1 CDS 74576952 74577134 74576952 ID=cds.MS.gene052412.t1;Parent=MS.gene052412.t1
chr6.1 exon 74577337 74577456 74577337 ID=MS.gene052412.t1.exon5;Parent=MS.gene052412.t1
chr6.1 CDS 74577337 74577456 74577337 ID=cds.MS.gene052412.t1;Parent=MS.gene052412.t1
Gene Sequence

>MS.gene052412

ATGTTTACTTCAAGGAAGAAAATCAGCAAGGACAAGGGCGCTGAGCCTACTGAATTTGAGGAGTCAGTTGGACAGGTTATATATTGATTACTACGGACTATTGTTGTTGCTGTGATTGGTGATTAGTCTCAATAATTACAAAATCTAAATCATTATTGTTTTCAATTTGTTGTTGCAGGCTTTGTTTGATCTGGAAAACACTAACCATGAGCTCAAAAGTGATCTGAAAGATTTATACATCAATTCTGCAGTGTAAGGATGTGTTCTTTATGGTTTGTTCGAAAATACATCTGTTTTTTTTTTGTGTCTCATGTCTACCGTTTTCTGTTATCTACAGCCAAGTTGATGTTTCTGGAAACCGCAAGGCTGTGGTTATCCATATCCCCTACAGATTGAGGAAGGGGTTCCGCAAAATTCACGTCAAGCTCGTTAGGGAGCTTGAGAAGAAATTCAGCGGAAAGGTAATCAAAGTCTTTTATACCTTTGCCAGTTATTCTTTCTAACTGCACCATCTTTTATTACGATTCTGTCTACCGACAATTTTTTGTTCCAATTTCTTTGTCTTCTACTCGTTATTTTTATCCTTATTTCAAGAACTTTATTCTGTTTTGCAATCTCCTTGGTTTTTAAATTGCTTTAATTTATATTTACTTGAGAGTAGTTCTATTCAGCTGTAAAACTGAAGTTAATCAGGCTGCGGTCTAAGCATGTGTTTGATTTGGCTTCATAGTTTTCATAATTACAACAAAAACTCACGATTACATGGAAGCTCCACTTTGTAGCTTCTCTTTATCACGACAGAATGCATGCCTTGTAATCATGCGAGTGGCAATGTAAAGAAGCCAAACCAAACATACACTAAATAATTTGATGATAGCATAACAATAGCCTTGAAAAATTGTTTTCTCACAGATTTATATTTGATGTTTCACTTTATTGTGTACTAATGAACCTTAAACTTGTAGGATGTAATCCTCATTGCCACCAGGAGAATCGTCAGGCCACCAAAGAAAGGCTCCGCTGCTCAGCGTCCACGCAGCCGTACACTCACTGCTGTCCACGAGGCAATGCTCGAGGATGTCGTGTTACCTGCTGAGATTGTTGGGAAACGTACTAGATACAGAATTGATGGATCCAAGATCATGAAGGTAAACTATACTAACAGCCAAACAATTTCCAAAAGTTACTCAAAGCTTGAATGAAAAATACATTTCGATTAAGCCAAACTAATGTGAAAATAAGCATACATGCTTTTATTATATACTACAATATTAAAATCATTAAGAAATGATGTTAATTCATTTCAAAAGAAATGATGTTAATTGAATTCTTGTGTTTCTGTGTATATAGGTGTTTTTGGACCCAAAGGAGAAAAACAATACTGAATACAAGCTCGAAACGTTTTCTGCAGTTTACAGGAAACTCTCAGGGAAAGATGTTGTGTTTGAGTACCCTGTTACTGAGGCGTAG

Protein sequence

>MS.gene052412.t1

MFTSRKKISKDKGAEPTEFEESVGQALFDLENTNHELKSDLKDLYINSAVQVDVSGNRKAVVIHIPYRLRKGFRKIHVKLVRELEKKFSGKDVILIATRRIVRPPKKGSAAQRPRSRTLTAVHEAMLEDVVLPAEIVGKRTRYRIDGSKIMKVFLDPKEKNNTEYKLETFSAVYRKLSGKDVVFEYPVTEA