AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62064

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62064.t1 MTR_4g131970 89.197 361 10 3 17 348 51 411 0.0 631
MS.gene62064.t1 MTR_2g022950 71.353 377 62 14 9 348 34 401 6.25e-164 464
MS.gene62064.t1 MTR_2g097960 51.724 348 108 7 33 325 8 350 1.14e-106 319
MS.gene62064.t1 MTR_3g101370 46.650 403 141 10 5 342 25 418 2.82e-105 315
MS.gene62064.t1 MTR_4g086600 52.805 303 84 5 37 288 113 407 6.66e-104 313
MS.gene62064.t1 MTR_1g020080 52.647 340 115 8 38 342 95 423 2.87e-103 310
MS.gene62064.t1 MTR_2g022950 68.421 209 32 6 9 189 34 236 2.03e-89 270
MS.gene62064.t1 MTR_2g022950 68.421 209 32 6 9 189 34 236 5.64e-89 269
MS.gene62064.t1 MTR_3g101370 42.857 273 93 6 5 217 25 294 1.71e-61 199
MS.gene62064.t1 MTR_1g020080 50.698 215 69 4 38 222 95 302 8.82e-60 195
MS.gene62064.t1 MTR_3g109090 28.746 327 184 8 2 289 11 327 2.78e-33 128
MS.gene62064.t1 MTR_3g109090 29.085 306 173 7 23 289 43 343 3.97e-33 127
MS.gene62064.t1 MTR_4g124940 27.348 362 205 8 13 337 19 359 2.46e-32 125
MS.gene62064.t1 MTR_8g022050 26.984 315 188 7 13 289 35 345 9.45e-29 115
MS.gene62064.t1 MTR_4g085540 25.368 272 173 7 42 286 124 392 3.67e-23 100
MS.gene62064.t1 MTR_4g085540 28.016 257 144 8 41 272 35 275 4.46e-22 97.8
MS.gene62064.t1 MTR_4g085540 26.705 176 97 4 119 280 35 192 3.65e-12 67.8
MS.gene62064.t1 MTR_1g097440 28.188 298 170 11 15 287 7 285 3.70e-22 98.2
MS.gene62064.t1 MTR_1g097440 25.000 264 165 8 42 276 120 379 3.06e-17 83.2
MS.gene62064.t1 MTR_7g102730 24.306 288 176 8 29 292 2 271 2.86e-21 95.5
MS.gene62064.t1 MTR_7g102730 24.113 282 184 7 32 286 90 368 6.63e-17 82.4
MS.gene62064.t1 MTR_1g019150 26.740 273 159 8 25 272 17 273 1.36e-20 93.2
MS.gene62064.t1 MTR_1g019150 25.000 272 173 8 32 275 112 380 7.21e-20 91.3
MS.gene62064.t1 MTR_1g019150 29.545 176 92 5 119 280 33 190 1.15e-12 69.3
MS.gene62064.t1 MTR_3g102040 28.016 257 144 8 41 272 25 265 3.47e-20 92.0
MS.gene62064.t1 MTR_3g102040 25.000 264 168 6 42 278 114 374 1.46e-17 84.3
MS.gene62064.t1 MTR_3g102040 29.050 179 89 6 119 280 25 182 1.20e-11 66.2
MS.gene62064.t1 MTR_6g040190 26.154 260 149 6 38 273 23 263 1.18e-19 90.5
MS.gene62064.t1 MTR_6g040190 23.358 274 183 7 42 291 115 385 8.58e-18 85.1
MS.gene62064.t1 MTR_7g106440 23.611 216 122 3 7 188 85 291 3.92e-18 84.3
MS.gene62064.t1 MTR_1g056520 28.814 177 94 4 40 185 108 283 1.64e-17 82.4
MS.gene62064.t1 MTR_1g061870 23.793 290 180 8 26 292 5 276 1.83e-17 83.6
MS.gene62064.t1 MTR_1g061870 24.621 264 165 8 42 278 105 361 3.63e-15 76.6
MS.gene62064.t1 MTR_7g104800 24.839 310 183 12 29 318 95 374 1.30e-16 80.9
MS.gene62064.t1 MTR_6g040070 23.282 262 171 7 42 276 17 275 4.27e-16 80.1
MS.gene62064.t1 MTR_3g075500 28.342 187 102 3 30 188 103 285 3.93e-15 75.1
MS.gene62064.t1 MTR_3g075500 26.829 205 117 6 99 283 89 280 2.35e-11 64.3
MS.gene62064.t1 MTR_4g104470 28.488 172 98 3 42 188 124 295 1.41e-14 73.6
MS.gene62064.t1 MTR_4g104470 25.269 186 106 4 115 282 119 289 8.94e-11 62.4
MS.gene62064.t1 MTR_4g052940 22.956 318 196 9 42 335 112 404 1.57e-14 74.7
MS.gene62064.t1 MTR_1g045510 28.000 175 104 2 36 188 109 283 4.26e-14 72.0
MS.gene62064.t1 MTR_1g064230 26.147 218 140 4 87 283 53 270 4.57e-14 72.0
MS.gene62064.t1 MTR_3g075500 29.240 171 89 3 30 172 103 269 1.27e-13 70.5
MS.gene62064.t1 MTR_5g016680 27.986 293 169 10 18 287 59 332 2.00e-13 70.9
MS.gene62064.t1 MTR_5g016680 27.986 293 168 10 18 287 59 331 7.92e-13 68.9
MS.gene62064.t1 MTR_7g118120 22.837 289 174 10 42 307 106 368 9.11e-13 69.3
MS.gene62064.t1 MTR_3g065190 25.595 168 106 2 41 189 26 193 1.49e-12 68.6
MS.gene62064.t1 MTR_3g448370 26.344 186 116 4 106 286 81 250 1.59e-12 68.9
MS.gene62064.t1 MTR_3g448370 26.344 186 116 4 106 286 186 355 1.80e-12 68.9
MS.gene62064.t1 MTR_3g448370 26.344 186 116 4 106 286 197 366 2.09e-12 68.6
MS.gene62064.t1 MTR_3g448370 26.344 186 116 4 106 286 186 355 2.31e-12 68.6
MS.gene62064.t1 MTR_3g448370 26.344 186 116 4 106 286 183 352 2.46e-12 68.6
MS.gene62064.t1 MTR_8g007390 23.346 257 161 8 42 276 216 458 6.24e-12 67.4
MS.gene62064.t1 MTR_8g007390 23.346 257 161 8 42 276 217 459 6.24e-12 67.4
MS.gene62064.t1 MTR_8g076100 24.031 258 159 10 42 276 420 663 1.29e-11 66.2
MS.gene62064.t1 MTR_3g027140 25.758 198 116 7 105 282 46 232 1.62e-11 63.9
MS.gene62064.t1 MTR_8g076100 24.031 258 159 10 42 276 420 663 1.66e-11 65.9
MS.gene62064.t1 MTR_8g076100 24.031 258 159 10 42 276 420 663 1.93e-11 65.9
MS.gene62064.t1 MTR_2g049640 26.404 178 110 4 114 286 192 353 3.38e-11 65.1
MS.gene62064.t1 MTR_3g098620 26.163 172 106 4 119 285 271 426 4.59e-11 64.7
MS.gene62064.t1 MTR_3g098620 26.163 172 106 4 119 285 271 426 4.80e-11 64.7
MS.gene62064.t1 MTR_3g098620 26.163 172 106 4 119 285 271 426 5.52e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62064.t1 AT1G11650 61.808 343 84 9 14 324 39 366 1.15e-141 407
MS.gene62064.t1 AT5G54900 61.250 320 94 7 33 324 53 370 6.62e-128 372
MS.gene62064.t1 AT4G27000 60.938 320 93 6 33 323 73 389 5.56e-118 347
MS.gene62064.t1 AT1G11650 62.681 276 66 6 14 260 39 306 8.18e-118 343
MS.gene62064.t1 AT3G19130 53.822 314 101 7 35 308 103 412 1.42e-105 317
MS.gene62064.t1 AT1G49600 52.424 330 112 6 34 326 113 434 2.34e-105 316
MS.gene62064.t1 AT5G19350 49.737 380 126 11 2 341 3 357 4.78e-103 310
MS.gene62064.t1 AT5G19350 50.000 384 123 12 2 341 3 361 5.64e-103 310
MS.gene62064.t1 AT1G49600 54.296 291 100 4 34 291 113 403 2.31e-99 301
MS.gene62064.t1 AT1G49600 54.296 291 100 4 34 291 113 403 2.31e-99 301
MS.gene62064.t1 AT1G47500 50.888 338 127 9 38 342 101 432 2.01e-95 290
MS.gene62064.t1 AT1G47490 50.592 338 128 9 38 342 99 430 2.62e-94 288
MS.gene62064.t1 AT1G47490 48.095 210 81 4 38 219 99 308 2.51e-52 176
MS.gene62064.t1 AT1G47490 26.630 184 101 5 119 282 102 271 8.64e-11 62.8
MS.gene62064.t1 AT1G47500 48.325 209 80 4 38 218 101 309 2.72e-52 176
MS.gene62064.t1 AT1G54080 28.440 327 188 8 1 289 28 346 4.23e-33 127
MS.gene62064.t1 AT1G54080 28.097 331 188 8 1 289 28 350 1.46e-32 126
MS.gene62064.t1 AT1G17370 27.564 312 187 7 13 289 27 334 2.79e-30 119
MS.gene62064.t1 AT1G17370 27.302 315 187 7 13 289 27 337 7.09e-30 118
MS.gene62064.t1 AT3G14100 28.254 315 184 8 13 289 32 342 4.55e-29 116
MS.gene62064.t1 AT2G23350 27.341 267 164 7 39 278 133 396 3.17e-25 107
MS.gene62064.t1 AT2G23350 28.516 256 144 8 41 272 47 287 1.04e-21 96.7
MS.gene62064.t1 AT4G34110 26.820 261 161 7 42 275 126 383 2.23e-24 104
MS.gene62064.t1 AT4G34110 28.516 256 144 7 41 272 37 277 5.83e-23 100
MS.gene62064.t1 AT4G34110 29.050 179 89 5 119 280 37 194 6.75e-14 73.2
MS.gene62064.t1 AT1G34140 25.182 274 179 6 42 291 33 304 2.77e-23 100
MS.gene62064.t1 AT1G22760 28.239 301 166 11 10 276 11 295 4.62e-21 94.7
MS.gene62064.t1 AT1G22760 25.283 265 164 8 42 276 138 398 1.84e-18 87.0
MS.gene62064.t1 AT1G49760 25.379 264 167 7 39 275 132 392 1.21e-20 93.6
MS.gene62064.t1 AT1G49760 26.562 256 149 6 41 272 46 286 6.60e-20 91.3
MS.gene62064.t1 AT1G49760 29.016 193 99 6 119 294 46 217 2.87e-13 71.2
MS.gene62064.t1 AT1G49760 25.379 264 167 7 39 275 132 392 1.21e-20 93.6
MS.gene62064.t1 AT1G49760 26.562 256 149 6 41 272 46 286 6.60e-20 91.3
MS.gene62064.t1 AT1G49760 29.016 193 99 6 119 294 46 217 2.87e-13 71.2
MS.gene62064.t1 AT3G16380 23.926 326 210 7 42 340 114 428 6.07e-20 91.3
MS.gene62064.t1 AT3G16380 23.970 267 160 5 41 280 22 272 2.24e-14 74.3
MS.gene62064.t1 AT1G71770 23.485 264 170 6 42 276 148 408 4.85e-19 89.0
MS.gene62064.t1 AT1G71770 23.485 264 170 6 42 276 148 408 4.85e-19 89.0
MS.gene62064.t1 AT2G36660 23.297 279 173 7 41 296 25 285 9.62e-18 84.7
MS.gene62064.t1 AT2G36660 24.275 276 181 8 28 278 100 372 6.40e-15 76.3
MS.gene62064.t1 AT3G52380 26.923 182 97 3 42 188 118 298 1.06e-16 80.5
MS.gene62064.t1 AT2G44710 24.514 257 158 6 42 276 216 458 1.06e-15 79.0
MS.gene62064.t1 AT2G44710 24.514 257 158 6 42 276 216 458 1.06e-15 79.0
MS.gene62064.t1 AT2G44710 24.514 257 158 6 42 276 131 373 1.18e-15 78.6
MS.gene62064.t1 AT4G24770 28.723 188 106 4 26 188 139 323 1.28e-15 77.0
MS.gene62064.t1 AT4G24770 28.723 188 106 4 26 188 139 323 1.28e-15 77.0
MS.gene62064.t1 AT5G50250 28.571 182 101 3 32 188 109 286 1.59e-15 76.3
MS.gene62064.t1 AT2G37220 29.851 201 117 6 115 291 88 288 1.67e-12 67.4
MS.gene62064.t1 AT1G60000 27.419 186 111 3 28 190 74 258 3.46e-12 66.2
MS.gene62064.t1 AT4G00830 20.678 295 192 8 42 307 118 399 4.91e-12 67.4
MS.gene62064.t1 AT2G18510 24.405 168 108 2 41 189 26 193 1.68e-11 65.1
MS.gene62064.t1 AT1G07360 27.536 138 87 2 204 328 217 354 2.00e-11 65.5
MS.gene62064.t1 AT1G29400 25.269 186 118 4 106 286 156 325 2.54e-11 65.5
MS.gene62064.t1 AT1G29400 25.269 186 118 4 106 286 156 325 2.54e-11 65.5
MS.gene62064.t1 AT1G29400 25.269 186 118 4 106 286 156 325 2.54e-11 65.5

Find 110 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACGCACAGTTGTATTATT+TGG 0.154719 8.2:-2657400 None:intergenic
TGTTGAGGAGGCTGGGTGTT+AGG 0.227357 8.2:-2654256 None:intergenic
TAATTTACCTGTCTGCAAAT+TGG 0.255811 8.2:-2657604 None:intergenic
CGGATAGCTGCCATACATAT+TGG 0.283199 8.2:-2658018 None:intergenic
AAATGTTCATCTGTGACATT+AGG 0.302859 8.2:-2657509 None:intergenic
GTTGAGGAGGCTGGGTGTTA+GGG 0.306198 8.2:-2654255 None:intergenic
GGATCGGTGATTTGCAATAT+TGG 0.312188 8.2:+2654326 MS.gene62064:CDS
TGGGTTTGTGGTTGAGGCTG+AGG 0.316930 8.2:-2654214 None:intergenic
GACAAAATGTCCGGCTTTCA+TGG 0.329353 8.2:+2657773 MS.gene62064:CDS
AATAATCAATCTGAAGGTTA+TGG 0.329662 8.2:+2655093 MS.gene62064:CDS
CGGCCGATTGTTGAGGAGGC+TGG 0.330912 8.2:-2654264 None:intergenic
ACCTGTTGTTTGCCGGGCTT+CGG 0.331167 8.2:-2657798 None:intergenic
CCTCAGCAGCAACAGCAATT+AGG 0.338496 8.2:+2658065 MS.gene62064:CDS
TCCACTGGTTTGGATCTTGT+TGG 0.341110 8.2:-2657917 None:intergenic
CCACTGGTTTGGATCTTGTT+GGG 0.344746 8.2:-2657916 None:intergenic
ATAATCAATCTGAAGGTTAT+GGG 0.351432 8.2:+2655094 MS.gene62064:CDS
AGCCAAGTCACCAACAAATA+TGG 0.357301 8.2:-2655266 None:intergenic
GGCCCACATCTGAGGAGGTT+GGG 0.362890 8.2:-2654233 None:intergenic
GGAGGTTGGGTTTGTGGTTG+AGG 0.368259 8.2:-2654220 None:intergenic
CACCATATTTGTTGGTGACT+TGG 0.380254 8.2:+2655264 MS.gene62064:CDS
CTACTACCATTCCACTGGTT+TGG 0.384001 8.2:-2657927 None:intergenic
CCGGATTACACCATATTTGT+TGG 0.386806 8.2:+2655256 MS.gene62064:CDS
ATCTGAGGAGGTTGGGTTTG+TGG 0.387938 8.2:-2654226 None:intergenic
ACTGGTAGGTCAAAAGGTTA+TGG 0.394870 8.2:+2655375 MS.gene62064:CDS
CTTGAGTTTATTAGCCGTGC+TGG 0.413195 8.2:+2655120 MS.gene62064:CDS
AGAGTACTTCAAACATATAA+TGG 0.420347 8.2:+2655150 MS.gene62064:CDS
TCCACAAGACCCATGCGTAT+TGG 0.423018 8.2:+2655465 MS.gene62064:CDS
GGATAGCTGCCATACATATT+GGG 0.430895 8.2:-2658017 None:intergenic
GCTTTCATGGGGCCGAAGCC+CGG 0.432030 8.2:+2657786 MS.gene62064:CDS
GGGCCCACATCTGAGGAGGT+TGG 0.435375 8.2:-2654234 None:intergenic
TATGCTTATGCCCCTCCTGC+TGG 0.436580 8.2:+2657987 MS.gene62064:CDS
GTTCCTGTCAATTACAACTT+TGG 0.446548 8.2:-2655352 None:intergenic
TTCTGCTGAGGAAGCACTAC+GGG 0.452001 8.2:+2657726 MS.gene62064:CDS
CTTGGCACACAGACTTCAAA+AGG 0.453306 8.2:+2655507 MS.gene62064:CDS
GGCCGATTGTTGAGGAGGCT+GGG 0.459511 8.2:-2654263 None:intergenic
CCGATGAAGTCAAAACTCTC+TGG 0.468589 8.2:+2654305 MS.gene62064:CDS
AGGAACACTGGTAGGTCAAA+AGG 0.474047 8.2:+2655369 MS.gene62064:CDS
CAATATGTATGGCAGCTATC+CGG 0.474348 8.2:+2658019 MS.gene62064:CDS
GTTCATGTTAAGATTCCATC+AGG 0.482409 8.2:+2657563 MS.gene62064:CDS
GAAGCGCCATGTTGATTCTC+CGG 0.492459 8.2:+2655237 MS.gene62064:CDS
AACCCACATCTCTTGCCTGA+TGG 0.492705 8.2:-2657578 None:intergenic
GTTTGTGGTTGAGGCTGAGG+AGG 0.495801 8.2:-2654211 None:intergenic
CCAACAAATATGGTGTAATC+CGG 0.495909 8.2:-2655256 None:intergenic
ACAAAATGTCCGGCTTTCAT+GGG 0.496290 8.2:+2657774 MS.gene62064:CDS
TTCTAGGTGGACAAAATGTC+CGG 0.503905 8.2:+2657764 MS.gene62064:CDS
GGTTTGTCCAATTTGCAGAC+AGG 0.504724 8.2:+2657597 MS.gene62064:CDS
GGCTTCGGCCCCATGAAAGC+CGG 0.508294 8.2:-2657783 None:intergenic
TTGACTTCATCGGCGCTGGT+AGG 0.508358 8.2:-2654295 None:intergenic
CGGTGATTTGCAATATTGGA+TGG 0.509387 8.2:+2654330 MS.gene62064:CDS
GGACAAGATCCCAATATGTA+TGG 0.511177 8.2:+2658008 MS.gene62064:CDS
TCCAATACGCATGGGTCTTG+TGG 0.514087 8.2:-2655466 None:intergenic
GTGCCAAAGTTGTAATTGAC+AGG 0.516979 8.2:+2655349 MS.gene62064:CDS
GTGTTAGGGGCCCACATCTG+AGG 0.522691 8.2:-2654241 None:intergenic
CCTAATTGCTGTTGCTGCTG+AGG 0.525948 8.2:-2658065 None:intergenic
TTGAGGAGGCTGGGTGTTAG+GGG 0.526232 8.2:-2654254 None:intergenic
ACTTCATCGGCGCTGGTAGG+AGG 0.526295 8.2:-2654292 None:intergenic
CACCCAGCCTCCTCAACAAT+CGG 0.527962 8.2:+2654261 MS.gene62064:CDS
ATATTGGGATCTTGTCCAGC+AGG 0.543892 8.2:-2658002 None:intergenic
AAACCCAACCTCCTCAGATG+TGG 0.548206 8.2:+2654230 MS.gene62064:CDS
TTCCATCAGGCAAGAGATGT+GGG 0.548464 8.2:+2657576 MS.gene62064:CDS
AATTGCTGTTGCTGCTGAGG+AGG 0.548730 8.2:-2658062 None:intergenic
AGAGGCTGGTCCAATACGCA+TGG 0.550256 8.2:-2655475 None:intergenic
TGTTAGTGATCATCATTTGA+CGG 0.550442 8.2:+2655294 MS.gene62064:CDS
AACCACTACTACCATTCCAC+TGG 0.555021 8.2:-2657932 None:intergenic
TCATCGGCGCTGGTAGGAGG+AGG 0.556417 8.2:-2654289 None:intergenic
GCTGTTGCTGCTGAGGAGGT+TGG 0.561779 8.2:-2658058 None:intergenic
GGAGGAACGGCCGATTGTTG+AGG 0.563253 8.2:-2654271 None:intergenic
AAGCTGAATAATCAATCTGA+AGG 0.566703 8.2:+2655087 MS.gene62064:CDS
ATTCCATCAGGCAAGAGATG+TGG 0.567746 8.2:+2657575 MS.gene62064:CDS
TATAATTGTTTCTCTCACAC+CGG 0.576751 8.2:+2654367 MS.gene62064:CDS
ATGTATGGCAGCTATCCGGC+GGG 0.578854 8.2:+2658023 MS.gene62064:CDS
GAAGTCAAAACTCTCTGGAT+CGG 0.582360 8.2:+2654310 MS.gene62064:CDS
GTTGTAATTGACAGGAACAC+TGG 0.586146 8.2:+2655357 MS.gene62064:CDS
AACCCAACCTCCTCAGATGT+GGG 0.594298 8.2:+2654231 MS.gene62064:CDS
CTGATGAGAGTGAACAAATG+AGG 0.596600 8.2:+2655412 MS.gene62064:CDS
TGGGATCTTGTCCAGCAGGA+GGG 0.598329 8.2:-2657998 None:intergenic
TTGGGATCTTGTCCAGCAGG+AGG 0.599370 8.2:-2657999 None:intergenic
GGAACGGCCGATTGTTGAGG+AGG 0.600132 8.2:-2654268 None:intergenic
GGGCGATGACTGAAATGCAA+GGG 0.605438 8.2:+2655433 MS.gene62064:CDS
TTATCAGAATCCACAAGGGG+GGG 0.605467 8.2:+2657360 MS.gene62064:CDS
TACTTCGGCGGATATGCCCA+AGG 0.609395 8.2:+2657954 MS.gene62064:CDS
GGGATCTTGTCCAGCAGGAG+GGG 0.611776 8.2:-2657997 None:intergenic
CAAGATCCAAACCAGTGGAA+TGG 0.612362 8.2:+2657921 MS.gene62064:CDS
GGCGCTGGTAGGAGGAGGAA+CGG 0.612810 8.2:-2654284 None:intergenic
TTAGGGGCCCACATCTGAGG+AGG 0.615784 8.2:-2654238 None:intergenic
AGGGCGATGACTGAAATGCA+AGG 0.618149 8.2:+2655432 MS.gene62064:CDS
CAAAATGTCCGGCTTTCATG+GGG 0.621663 8.2:+2657775 MS.gene62064:CDS
AACCAGTGGAATGGTAGTAG+TGG 0.621693 8.2:+2657930 MS.gene62064:CDS
GTTCTGCTGAGGAAGCACTA+CGG 0.622035 8.2:+2657725 MS.gene62064:CDS
AATGGTAGTAGTGGTTACTT+CGG 0.624503 8.2:+2657939 MS.gene62064:CDS
ATATATCAGGAGTTCTGCTG+AGG 0.625988 8.2:+2657714 MS.gene62064:intron
TGGTAACTTGCATACCCCGC+CGG 0.629209 8.2:-2658038 None:intergenic
TAATTGACAGGAACACTGGT+AGG 0.634168 8.2:+2655361 MS.gene62064:CDS
GAGGCTGGTCCAATACGCAT+GGG 0.635007 8.2:-2655474 None:intergenic
CTTATCAGAATCCACAAGGG+GGG 0.644542 8.2:+2657359 MS.gene62064:CDS
TTCTTATCAGAATCCACAAG+GGG 0.645949 8.2:+2657357 MS.gene62064:CDS
GGTAGTAGTGGTTACTTCGG+CGG 0.648555 8.2:+2657942 MS.gene62064:CDS
TATGTATGGCAGCTATCCGG+CGG 0.650038 8.2:+2658022 MS.gene62064:CDS
GGCGATGACTGAAATGCAAG+GGG 0.651248 8.2:+2655434 MS.gene62064:CDS
TTAAATGGTACGCTTCTAGG+TGG 0.651437 8.2:+2657751 MS.gene62064:CDS
GCTTCTTATCAGAATCCACA+AGG 0.654850 8.2:+2657355 MS.gene62064:intron
TGATGAGAGTGAACAAATGA+GGG 0.656259 8.2:+2655413 MS.gene62064:CDS
CCCAACAAGATCCAAACCAG+TGG 0.656471 8.2:+2657916 MS.gene62064:CDS
AAGCGGTTATTATACCTCGC+CGG 0.663738 8.2:-2654386 None:intergenic
TTGTTTCTCTCACACCGGCG+AGG 0.669836 8.2:+2654372 MS.gene62064:CDS
GTACTCTCTCAGCACCAGCA+CGG 0.677779 8.2:-2655134 None:intergenic
TGTAATCCGGAGAATCAACA+TGG 0.693267 8.2:-2655243 None:intergenic
CTTCTTATCAGAATCCACAA+GGG 0.694873 8.2:+2657356 MS.gene62064:CDS
TCTTATCAGAATCCACAAGG+GGG 0.720937 8.2:+2657358 MS.gene62064:CDS
TGTATGGCAGCTATCCGGCG+GGG 0.738355 8.2:+2658024 MS.gene62064:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTATTTTTATTGAATATTT+AGG + chr8.2:2656742-2656761 MS.gene62064:intron 10.0%
!!! ATTTTATTATATTATGCTAT+TGG + chr8.2:2657246-2657265 MS.gene62064:intron 10.0%
!! AAAAGATATGATAATAAAAG+CGG - chr8.2:2654406-2654425 None:intergenic 15.0%
!! ATTTGATATTTATCTTATGA+CGG + chr8.2:2657643-2657662 MS.gene62064:intron 15.0%
!! GTTAACTTTGATTATAATAT+AGG + chr8.2:2656595-2656614 MS.gene62064:intron 15.0%
!!! AATTATTATGTCAATTTTGA+AGG + chr8.2:2654995-2655014 MS.gene62064:intron 15.0%
!!! CTTGTTTACTTTAATAATTT+TGG + chr8.2:2656234-2656253 MS.gene62064:intron 15.0%
!!! TATAGATTTTAAATCTCTTA+GGG + chr8.2:2654432-2654451 MS.gene62064:intron 15.0%
!!! TATTTATTTTCATGATGTTA+TGG + chr8.2:2656396-2656415 MS.gene62064:intron 15.0%
!!! TTTTTGTAGAAAAATTGTTA+GGG + chr8.2:2654468-2654487 MS.gene62064:intron 15.0%
!!! TTTTTTGTAGAAAAATTGTT+AGG + chr8.2:2654467-2654486 MS.gene62064:intron 15.0%
!! ATTGAACTTTGATTATAACA+AGG + chr8.2:2656630-2656649 MS.gene62064:intron 20.0%
!! GCATAATATAATAAAATGCA+CGG - chr8.2:2657243-2657262 None:intergenic 20.0%
!! GTAATTTCGTTTCAAAATTT+GGG + chr8.2:2655759-2655778 MS.gene62064:intron 20.0%
!! TCCTGATATATTAAATATAG+TGG - chr8.2:2657705-2657724 None:intergenic 20.0%
!! TTCATCTATACATTAAATCA+TGG - chr8.2:2656915-2656934 None:intergenic 20.0%
!! TTTACATATGCATATAGAAA+TGG - chr8.2:2656785-2656804 None:intergenic 20.0%
!!! AAATTGTTCTCTTTTATACT+TGG + chr8.2:2657438-2657457 MS.gene62064:intron 20.0%
!!! CTATAGATTTTAAATCTCTT+AGG + chr8.2:2654431-2654450 MS.gene62064:intron 20.0%
!!! GGTGTTTTTGTTTATATTTA+AGG + chr8.2:2655016-2655035 MS.gene62064:intron 20.0%
!!! TATTGTTTAATTTGAATACG+AGG + chr8.2:2654544-2654563 MS.gene62064:intron 20.0%
!!! TCAAAAGGTTGTTTTTTTAA+TGG + chr8.2:2655522-2655541 MS.gene62064:intron 20.0%
!!! TGACTTATTTTGATCAATAA+TGG + chr8.2:2657872-2657891 MS.gene62064:intron 20.0%
!!! TTGGATAATCTTTTATTCAT+TGG + chr8.2:2654620-2654639 MS.gene62064:intron 20.0%
!!! TTGGATTTAATTTGTTTACA+CGG + chr8.2:2654639-2654658 MS.gene62064:intron 20.0%
!!! TTTTAATGGTTGATATACAT+TGG + chr8.2:2655536-2655555 MS.gene62064:intron 20.0%
! AAAATGCCACCAAAAATTAT+GGG - chr8.2:2655946-2655965 None:intergenic 25.0%
! AAAGAGAAAGTAAACAGAAT+AGG - chr8.2:2655990-2656009 None:intergenic 25.0%
! AACTCGTATAATTCAGTTAA+GGG + chr8.2:2655327-2655346 MS.gene62064:intron 25.0%
! AAGATAAATGACATAGAACA+TGG - chr8.2:2657308-2657327 None:intergenic 25.0%
! AATAATCAATCTGAAGGTTA+TGG + chr8.2:2655093-2655112 MS.gene62064:CDS 25.0%
! ATAATCAATCTGAAGGTTAT+GGG + chr8.2:2655094-2655113 MS.gene62064:CDS 25.0%
! ATACTGAAGGATAACATTTA+GGG - chr8.2:2655682-2655701 None:intergenic 25.0%
! ATGCAAGAACTAAGATTATT+TGG - chr8.2:2656161-2656180 None:intergenic 25.0%
! ATTAGAAACCAAGCTATATA+TGG + chr8.2:2654843-2654862 MS.gene62064:intron 25.0%
! CAGTATCATTATTCATCAAT+AGG - chr8.2:2655612-2655631 None:intergenic 25.0%
! CGTAATTTCGTTTCAAAATT+TGG + chr8.2:2655758-2655777 MS.gene62064:intron 25.0%
! CTTGAACTGTTTATTATTCA+AGG + chr8.2:2654932-2654951 MS.gene62064:intron 25.0%
! GAAAACTTATCTTATACTGA+AGG - chr8.2:2655695-2655714 None:intergenic 25.0%
! GCCACTATATTTAATATATC+AGG + chr8.2:2657701-2657720 MS.gene62064:intron 25.0%
! TATACTGAAGGATAACATTT+AGG - chr8.2:2655683-2655702 None:intergenic 25.0%
! TCTTCTCTTATATTTCAACA+AGG + chr8.2:2656941-2656960 MS.gene62064:intron 25.0%
! TGATGAATAATGATACTGTT+TGG + chr8.2:2655614-2655633 MS.gene62064:intron 25.0%
! TGCATGAAGTTTATGTTAAT+CGG + chr8.2:2654499-2654518 MS.gene62064:intron 25.0%
!! AAACAAGTTTTTACTCAGTA+TGG + chr8.2:2657533-2657552 MS.gene62064:CDS 25.0%
!! AAGCATAATTTTCATAACCT+TGG - chr8.2:2657974-2657993 None:intergenic 25.0%
!! AGAGTACTTCAAACATATAA+TGG + chr8.2:2655150-2655169 MS.gene62064:CDS 25.0%
!! AGCATAATTTTCATAACCTT+GGG - chr8.2:2657973-2657992 None:intergenic 25.0%
!! TATAAGATAAGTTTTCAGTC+AGG + chr8.2:2655699-2655718 MS.gene62064:intron 25.0%
!! TTATTGAATATTTAGGACAC+AGG + chr8.2:2656749-2656768 MS.gene62064:intron 25.0%
!!! ATTTAATTTGTTTACACGGA+CGG + chr8.2:2654643-2654662 MS.gene62064:intron 25.0%
!!! TGTTTTCTGTTTTGAGAAAT+TGG + chr8.2:2654601-2654620 MS.gene62064:intron 25.0%
AAAATCTGTACTATGCAGAT+TGG + chr8.2:2656350-2656369 MS.gene62064:intron 30.0%
AAATGTTCATCTGTGACATT+AGG - chr8.2:2657512-2657531 None:intergenic 30.0%
AAGCTGAATAATCAATCTGA+AGG + chr8.2:2655087-2655106 MS.gene62064:CDS 30.0%
AATAGCTGCTGTATGTAATT+TGG - chr8.2:2656042-2656061 None:intergenic 30.0%
ATACGCACAGTTGTATTATT+TGG - chr8.2:2657403-2657422 None:intergenic 30.0%
ATGCTATCAAATCATGATAC+GGG + chr8.2:2654688-2654707 MS.gene62064:intron 30.0%
ATTGAAATAAAGAACAGAGC+AGG - chr8.2:2656569-2656588 None:intergenic 30.0%
CATATATTGCTCAGTCTAAT+GGG + chr8.2:2656475-2656494 MS.gene62064:intron 30.0%
CATGCTATCAAATCATGATA+CGG + chr8.2:2654687-2654706 MS.gene62064:intron 30.0%
CATGTAATTGTTTGAATCGA+TGG + chr8.2:2656878-2656897 MS.gene62064:intron 30.0%
CTCTTATATTTCAACAAGGA+TGG + chr8.2:2656945-2656964 MS.gene62064:intron 30.0%
GAAAATGCCACCAAAAATTA+TGG - chr8.2:2655947-2655966 None:intergenic 30.0%
GAACTCGTATAATTCAGTTA+AGG + chr8.2:2655326-2655345 MS.gene62064:intron 30.0%
GTTGTTTATGAATCGATGTA+TGG + chr8.2:2654720-2654739 MS.gene62064:intron 30.0%
TAAATGACATAGAACATGGA+AGG - chr8.2:2657304-2657323 None:intergenic 30.0%
TAATTTACCTGTCTGCAAAT+TGG - chr8.2:2657607-2657626 None:intergenic 30.0%
TCACACGATAAAAGATAACA+GGG - chr8.2:2655657-2655676 None:intergenic 30.0%
TCGATGTATGGTTTATTTGT+TGG + chr8.2:2654732-2654751 MS.gene62064:intron 30.0%
TGTTAGTGATCATCATTTGA+CGG + chr8.2:2655294-2655313 MS.gene62064:CDS 30.0%
TTAAGAGCTGAAACAAAGAA+AGG - chr8.2:2656092-2656111 None:intergenic 30.0%
TTCACACGATAAAAGATAAC+AGG - chr8.2:2655658-2655677 None:intergenic 30.0%
TTCATCAATAGGTTAAACAC+TGG - chr8.2:2655601-2655620 None:intergenic 30.0%
! ACATCGTTTTCATAACAAAC+TGG - chr8.2:2654584-2654603 None:intergenic 30.0%
! GCACAAGTTTTTCTTTATGT+TGG - chr8.2:2655562-2655581 None:intergenic 30.0%
! TATAATTGTTTCTCTCACAC+CGG + chr8.2:2654367-2654386 MS.gene62064:CDS 30.0%
! TCTTTGTTGCAGATTTTTGT+TGG + chr8.2:2657476-2657495 MS.gene62064:intron 30.0%
!! ATCTATTGTCGTGATTTGTT+AGG - chr8.2:2654804-2654823 None:intergenic 30.0%
!! TGTTATGGCTTTTTCTTTCT+TGG + chr8.2:2656411-2656430 MS.gene62064:intron 30.0%
!! TTAAAGTGTTGAGAAATGCA+AGG - chr8.2:2656723-2656742 None:intergenic 30.0%
!!! CAAAAGGTTATGGTTTTGTT+AGG + chr8.2:2655385-2655404 MS.gene62064:CDS 30.0%
AATGGTAGTAGTGGTTACTT+CGG + chr8.2:2657939-2657958 MS.gene62064:CDS 35.0%
AATTATGGGCGAGACATAAA+CGG - chr8.2:2655932-2655951 None:intergenic 35.0%
ATCTGCAACAAAGAGAAATC+AGG - chr8.2:2657470-2657489 None:intergenic 35.0%
CCAACAAATATGGTGTAATC+CGG - chr8.2:2655259-2655278 None:intergenic 35.0%
CCAGAATTTCAGATTGAACT+GGG + chr8.2:2655194-2655213 MS.gene62064:intron 35.0%
CTTCTTATCAGAATCCACAA+GGG + chr8.2:2657356-2657375 MS.gene62064:CDS 35.0%
GAAAGTAAACAGAATAGGTG+CGG - chr8.2:2655985-2656004 None:intergenic 35.0%
GCATATATTGCTCAGTCTAA+TGG + chr8.2:2656474-2656493 MS.gene62064:intron 35.0%
GTTCATGTTAAGATTCCATC+AGG + chr8.2:2657563-2657582 MS.gene62064:CDS 35.0%
GTTCCTGTCAATTACAACTT+TGG - chr8.2:2655355-2655374 None:intergenic 35.0%
TGATGAGAGTGAACAAATGA+GGG + chr8.2:2655413-2655432 MS.gene62064:CDS 35.0%
TGCATCGAAGCATATAGAAA+TGG - chr8.2:2656666-2656685 None:intergenic 35.0%
TGCTATCAAATCATGATACG+GGG + chr8.2:2654689-2654708 MS.gene62064:intron 35.0%
TTCTCACCAGAACTAAAAGT+TGG - chr8.2:2655220-2655239 None:intergenic 35.0%
TTCTTATCAGAATCCACAAG+GGG + chr8.2:2657357-2657376 MS.gene62064:CDS 35.0%
TTGAGGAGCAATAAAAAGGT+CGG - chr8.2:2656984-2657003 None:intergenic 35.0%
! AACTATTACCTGTTGTTTGC+CGG - chr8.2:2657808-2657827 None:intergenic 35.0%
! CTCTTTTCCTTTCGTTTTGT+TGG + chr8.2:2656004-2656023 MS.gene62064:intron 35.0%
! CTTTCGAGCTTTACTTCTTT+TGG + chr8.2:2656120-2656139 MS.gene62064:intron 35.0%
! GAGTTGTTCTGTTTCTTTGT+AGG + chr8.2:2655038-2655057 MS.gene62064:intron 35.0%
! GCAGATTTTTGTTGGCAATT+TGG + chr8.2:2657484-2657503 MS.gene62064:intron 35.0%
! TAATGGGTAGTGTAGTCTTT+CGG + chr8.2:2656491-2656510 MS.gene62064:intron 35.0%
! TATGTCTCGCCCATAATTTT+TGG + chr8.2:2655934-2655953 MS.gene62064:intron 35.0%
! TGTTCTGTTTCTTTGTAGGT+TGG + chr8.2:2655042-2655061 MS.gene62064:intron 35.0%
! TTTTACTCAGTATGGTGAAC+TGG + chr8.2:2657541-2657560 MS.gene62064:CDS 35.0%
!! AATCAGAGCACTGAATAGAA+TGG - chr8.2:2657679-2657698 None:intergenic 35.0%
!! ATAAACGGATTACTGACCTA+CGG - chr8.2:2655917-2655936 None:intergenic 35.0%
!! ATCAGAGCACTGAATAGAAT+GGG - chr8.2:2657678-2657697 None:intergenic 35.0%
!! GTTTTAAATGGTACGCTTCT+AGG + chr8.2:2657748-2657767 MS.gene62064:CDS 35.0%
AAGCAAGCCAACAAAACGAA+AGG - chr8.2:2656014-2656033 None:intergenic 40.0%
AATGGCACCATTATGCCAAA+TGG + chr8.2:2655168-2655187 MS.gene62064:intron 40.0%
ACAAAATGTCCGGCTTTCAT+GGG + chr8.2:2657774-2657793 MS.gene62064:CDS 40.0%
ACAGAATAGGTGCGGAATAA+AGG - chr8.2:2655977-2655996 None:intergenic 40.0%
ACTGGTAGGTCAAAAGGTTA+TGG + chr8.2:2655375-2655394 MS.gene62064:CDS 40.0%
AGAGCTGAAACAAAGAAAGG+TGG - chr8.2:2656089-2656108 None:intergenic 40.0%
AGCCAAGTCACCAACAAATA+TGG - chr8.2:2655269-2655288 None:intergenic 40.0%
AGGGTTGAGGAGCAATAAAA+AGG - chr8.2:2656988-2657007 None:intergenic 40.0%
ATATATCAGGAGTTCTGCTG+AGG + chr8.2:2657714-2657733 MS.gene62064:intron 40.0%
CAATATGTATGGCAGCTATC+CGG + chr8.2:2658019-2658038 MS.gene62064:CDS 40.0%
CCAAGCTATATATGGCACTA+TGG + chr8.2:2654851-2654870 MS.gene62064:intron 40.0%
CCATAGTGCCATATATAGCT+TGG - chr8.2:2654854-2654873 None:intergenic 40.0%
CCGGATTACACCATATTTGT+TGG + chr8.2:2655256-2655275 MS.gene62064:CDS 40.0%
CTGATGAGAGTGAACAAATG+AGG + chr8.2:2655412-2655431 MS.gene62064:CDS 40.0%
GAAGTCAAAACTCTCTGGAT+CGG + chr8.2:2654310-2654329 MS.gene62064:CDS 40.0%
GAGCTGAAACAAAGAAAGGT+GGG - chr8.2:2656088-2656107 None:intergenic 40.0%
GCCAGAATTTCAGATTGAAC+TGG + chr8.2:2655193-2655212 MS.gene62064:intron 40.0%
GCTTCTTATCAGAATCCACA+AGG + chr8.2:2657355-2657374 MS.gene62064:intron 40.0%
GGACAAGATCCCAATATGTA+TGG + chr8.2:2658008-2658027 MS.gene62064:CDS 40.0%
GGATAGCTGCCATACATATT+GGG - chr8.2:2658020-2658039 None:intergenic 40.0%
GTGCCAAAGTTGTAATTGAC+AGG + chr8.2:2655349-2655368 MS.gene62064:CDS 40.0%
GTTACCAATCTCAAATCCAC+CGG + chr8.2:2656286-2656305 MS.gene62064:intron 40.0%
GTTGTAATTGACAGGAACAC+TGG + chr8.2:2655357-2655376 MS.gene62064:CDS 40.0%
TAATTGACAGGAACACTGGT+AGG + chr8.2:2655361-2655380 MS.gene62064:CDS 40.0%
TCTTATCAGAATCCACAAGG+GGG + chr8.2:2657358-2657377 MS.gene62064:CDS 40.0%
TGTAATCCGGAGAATCAACA+TGG - chr8.2:2655246-2655265 None:intergenic 40.0%
TTAAATGGTACGCTTCTAGG+TGG + chr8.2:2657751-2657770 MS.gene62064:CDS 40.0%
TTACCAATCTCAAATCCACC+GGG + chr8.2:2656287-2656306 MS.gene62064:intron 40.0%
TTCTAGGTGGACAAAATGTC+CGG + chr8.2:2657764-2657783 MS.gene62064:CDS 40.0%
TTTCAGCTGAAATCAGTTCC+AGG - chr8.2:2655869-2655888 None:intergenic 40.0%
! ACTATTACCTGTTGTTTGCC+GGG - chr8.2:2657807-2657826 None:intergenic 40.0%
! CCCAGTTCAATCTGAAATTC+TGG - chr8.2:2655197-2655216 None:intergenic 40.0%
! CGGTGATTTGCAATATTGGA+TGG + chr8.2:2654330-2654349 MS.gene62064:CDS 40.0%
! GGAACTGATTTCAGCTGAAA+TGG + chr8.2:2655869-2655888 MS.gene62064:intron 40.0%
! GGATCGGTGATTTGCAATAT+TGG + chr8.2:2654326-2654345 MS.gene62064:CDS 40.0%
! TGTGCCAAGGTTTTTGTTAG+AGG - chr8.2:2655496-2655515 None:intergenic 40.0%
! TTTTATTGCTCCTCAACCCT+GGG + chr8.2:2656988-2657007 MS.gene62064:intron 40.0%
! TTTTTATTGCTCCTCAACCC+TGG + chr8.2:2656987-2657006 MS.gene62064:intron 40.0%
!! CACCATATTTGTTGGTGACT+TGG + chr8.2:2655264-2655283 MS.gene62064:CDS 40.0%
!! TCAGAGCACTGAATAGAATG+GGG - chr8.2:2657677-2657696 None:intergenic 40.0%
!! TGGTTAGTTTCTGCACTGTT+TGG + chr8.2:2656431-2656450 MS.gene62064:intron 40.0%
!!! CCAGAGAGTTTTGACTTCAT+CGG - chr8.2:2654308-2654327 None:intergenic 40.0%
!!! GAAGCACTACGGGTTTTAAA+TGG + chr8.2:2657736-2657755 MS.gene62064:CDS 40.0%
AAATCCACCGGGTTAAAAGG+TGG + chr8.2:2656298-2656317 MS.gene62064:intron 45.0%
AACCACTACTACCATTCCAC+TGG - chr8.2:2657935-2657954 None:intergenic 45.0%
AACCAGTGGAATGGTAGTAG+TGG + chr8.2:2657930-2657949 MS.gene62064:CDS 45.0%
AACTCGTGTTGTCTCAAAGC+AGG + chr8.2:2655817-2655836 MS.gene62064:intron 45.0%
AAGCGGTTATTATACCTCGC+CGG - chr8.2:2654389-2654408 None:intergenic 45.0%
AATAGGTGCGGAATAAAGGC+GGG - chr8.2:2655973-2655992 None:intergenic 45.0%
AATCCACCGGGTTAAAAGGT+GGG + chr8.2:2656299-2656318 MS.gene62064:intron 45.0%
ACTCGTGTTGTCTCAAAGCA+GGG + chr8.2:2655818-2655837 MS.gene62064:intron 45.0%
AGGAACACTGGTAGGTCAAA+AGG + chr8.2:2655369-2655388 MS.gene62064:CDS 45.0%
ATATTGGGATCTTGTCCAGC+AGG - chr8.2:2658005-2658024 None:intergenic 45.0%
ATTCCAAAAGCAAGCACACG+AGG - chr8.2:2657117-2657136 None:intergenic 45.0%
ATTCCATCAGGCAAGAGATG+TGG + chr8.2:2657575-2657594 MS.gene62064:CDS 45.0%
CAAAATGTCCGGCTTTCATG+GGG + chr8.2:2657775-2657794 MS.gene62064:CDS 45.0%
CAACAAGGATGGTGTGTGTA+AGG + chr8.2:2656956-2656975 MS.gene62064:intron 45.0%
CAAGATCCAAACCAGTGGAA+TGG + chr8.2:2657921-2657940 MS.gene62064:CDS 45.0%
CCAGCCTCTAACAAAAACCT+TGG + chr8.2:2655489-2655508 MS.gene62064:CDS 45.0%
CCGATGAAGTCAAAACTCTC+TGG + chr8.2:2654305-2654324 MS.gene62064:CDS 45.0%
CGGATAGCTGCCATACATAT+TGG - chr8.2:2658021-2658040 None:intergenic 45.0%
CTCAAATCCACCGGGTTAAA+AGG + chr8.2:2656295-2656314 MS.gene62064:intron 45.0%
CTGAAATTCTGGCCACCATT+TGG - chr8.2:2655186-2655205 None:intergenic 45.0%
CTTATCAGAATCCACAAGGG+GGG + chr8.2:2657359-2657378 MS.gene62064:CDS 45.0%
CTTGAGTTTATTAGCCGTGC+TGG + chr8.2:2655120-2655139 MS.gene62064:CDS 45.0%
CTTGGCACACAGACTTCAAA+AGG + chr8.2:2655507-2655526 MS.gene62064:CDS 45.0%
GAATAGGTGCGGAATAAAGG+CGG - chr8.2:2655974-2655993 None:intergenic 45.0%
GACAAAATGTCCGGCTTTCA+TGG + chr8.2:2657773-2657792 MS.gene62064:CDS 45.0%
GGTTAAACACTGGCATCTGA+AGG - chr8.2:2655591-2655610 None:intergenic 45.0%
GGTTTGTCCAATTTGCAGAC+AGG + chr8.2:2657597-2657616 MS.gene62064:CDS 45.0%
GTTGGGTCTGAAAATCAGCA+AGG - chr8.2:2657902-2657921 None:intergenic 45.0%
GTTTGCATCAAATGCAGCTC+AGG - chr8.2:2657847-2657866 None:intergenic 45.0%
TCTCAAAGCAGGGTTTCAAG+AGG + chr8.2:2655828-2655847 MS.gene62064:intron 45.0%
TGCAATGAATGATGCACCCA+GGG - chr8.2:2657007-2657026 None:intergenic 45.0%
TTATCAGAATCCACAAGGGG+GGG + chr8.2:2657360-2657379 MS.gene62064:CDS 45.0%
TTCCATCAGGCAAGAGATGT+GGG + chr8.2:2657576-2657595 MS.gene62064:CDS 45.0%
! ACGGAAGGCATGCACTAAAA+TGG - chr8.2:2655898-2655917 None:intergenic 45.0%
! ATTCCTCGTGTGCTTGCTTT+TGG + chr8.2:2657111-2657130 MS.gene62064:intron 45.0%
! CCAAGGTTTTTGTTAGAGGC+TGG - chr8.2:2655492-2655511 None:intergenic 45.0%
! CTACTACCATTCCACTGGTT+TGG - chr8.2:2657930-2657949 None:intergenic 45.0%
! TAACCCGGTGGATTTGAGAT+TGG - chr8.2:2656293-2656312 None:intergenic 45.0%
! TTTTAGTGCATGCCTTCCGT+AGG + chr8.2:2655898-2655917 MS.gene62064:intron 45.0%
!! CAACGAAAGCACTAAAGACC+AGG - chr8.2:2656327-2656346 None:intergenic 45.0%
!! CCACTGGTTTGGATCTTGTT+GGG - chr8.2:2657919-2657938 None:intergenic 45.0%
!! GTCTCGCCCATAATTTTTGG+TGG + chr8.2:2655937-2655956 MS.gene62064:intron 45.0%
!! TCCACTGGTTTGGATCTTGT+TGG - chr8.2:2657920-2657939 None:intergenic 45.0%
!!! ACTGGGCCAACTTTTAGTTC+TGG + chr8.2:2655211-2655230 MS.gene62064:intron 45.0%
!!! CTTTTGAAGTCTGTGTGCCA+AGG - chr8.2:2655509-2655528 None:intergenic 45.0%
AAACCCAACCTCCTCAGATG+TGG + chr8.2:2654230-2654249 MS.gene62064:CDS 50.0%
AACCCAACCTCCTCAGATGT+GGG + chr8.2:2654231-2654250 MS.gene62064:CDS 50.0%
AACCCACATCTCTTGCCTGA+TGG - chr8.2:2657581-2657600 None:intergenic 50.0%
AGGGCGATGACTGAAATGCA+AGG + chr8.2:2655432-2655451 MS.gene62064:CDS 50.0%
ATCCACCGGGTTAAAAGGTG+GGG + chr8.2:2656300-2656319 MS.gene62064:intron 50.0%
ATCTGAGGAGGTTGGGTTTG+TGG - chr8.2:2654229-2654248 None:intergenic 50.0%
CCCAACAAGATCCAAACCAG+TGG + chr8.2:2657916-2657935 MS.gene62064:CDS 50.0%
CCTCAGCAGCAACAGCAATT+AGG + chr8.2:2658065-2658084 MS.gene62064:CDS 50.0%
CTGGCCACCATTTGGCATAA+TGG - chr8.2:2655178-2655197 None:intergenic 50.0%
GGCACCATTATGCCAAATGG+TGG + chr8.2:2655171-2655190 MS.gene62064:intron 50.0%
GGCGATGACTGAAATGCAAG+GGG + chr8.2:2655434-2655453 MS.gene62064:CDS 50.0%
GGGCGATGACTGAAATGCAA+GGG + chr8.2:2655433-2655452 MS.gene62064:CDS 50.0%
GGTAGTAGTGGTTACTTCGG+CGG + chr8.2:2657942-2657961 MS.gene62064:CDS 50.0%
GTGCAATGAATGATGCACCC+AGG - chr8.2:2657008-2657027 None:intergenic 50.0%
TATGTATGGCAGCTATCCGG+CGG + chr8.2:2658022-2658041 MS.gene62064:CDS 50.0%
TCCAATACGCATGGGTCTTG+TGG - chr8.2:2655469-2655488 None:intergenic 50.0%
TCCACAAGACCCATGCGTAT+TGG + chr8.2:2655465-2655484 MS.gene62064:CDS 50.0%
! AATTGCTGTTGCTGCTGAGG+AGG - chr8.2:2658065-2658084 None:intergenic 50.0%
! ACGGATTACTGACCTACGGA+AGG - chr8.2:2655913-2655932 None:intergenic 50.0%
! CCTAATTGCTGTTGCTGCTG+AGG - chr8.2:2658068-2658087 None:intergenic 50.0%
!! GAAGCGCCATGTTGATTCTC+CGG + chr8.2:2655237-2655256 MS.gene62064:CDS 50.0%
!! GTTCTGCTGAGGAAGCACTA+CGG + chr8.2:2657725-2657744 MS.gene62064:CDS 50.0%
!! TTCTGCTGAGGAAGCACTAC+GGG + chr8.2:2657726-2657745 MS.gene62064:CDS 50.0%
!!! AGTTTTGACTTCATCGGCGC+TGG - chr8.2:2654302-2654321 None:intergenic 50.0%
AGGCTCTATCATCTCGAGCC+TGG + chr8.2:2655848-2655867 MS.gene62064:intron 55.0%
ATGTATGGCAGCTATCCGGC+GGG + chr8.2:2658023-2658042 MS.gene62064:CDS 55.0%
CACCCAGCCTCCTCAACAAT+CGG + chr8.2:2654261-2654280 MS.gene62064:CDS 55.0%
GAATGATGCACCCAGGGTTG+AGG - chr8.2:2657001-2657020 None:intergenic 55.0%
GGAGGTTGGGTTTGTGGTTG+AGG - chr8.2:2654223-2654242 None:intergenic 55.0%
GTACTCTCTCAGCACCAGCA+CGG - chr8.2:2655137-2655156 None:intergenic 55.0%
GTTGAGGAGGCTGGGTGTTA+GGG - chr8.2:2654258-2654277 None:intergenic 55.0%
GTTTGTGGTTGAGGCTGAGG+AGG - chr8.2:2654214-2654233 None:intergenic 55.0%
TACTTCGGCGGATATGCCCA+AGG + chr8.2:2657954-2657973 MS.gene62064:CDS 55.0%
TATGCTTATGCCCCTCCTGC+TGG + chr8.2:2657987-2658006 MS.gene62064:CDS 55.0%
TGGGATCTTGTCCAGCAGGA+GGG - chr8.2:2658001-2658020 None:intergenic 55.0%
TGGGTTTGTGGTTGAGGCTG+AGG - chr8.2:2654217-2654236 None:intergenic 55.0%
TGTTGAGGAGGCTGGGTGTT+AGG - chr8.2:2654259-2654278 None:intergenic 55.0%
TTGAGGAGGCTGGGTGTTAG+GGG - chr8.2:2654257-2654276 None:intergenic 55.0%
TTGGGATCTTGTCCAGCAGG+AGG - chr8.2:2658002-2658021 None:intergenic 55.0%
! ACCTGTTGTTTGCCGGGCTT+CGG - chr8.2:2657801-2657820 None:intergenic 55.0%
! AGAGGCTGGTCCAATACGCA+TGG - chr8.2:2655478-2655497 None:intergenic 55.0%
! CAGGACCCCACCTTTTAACC+CGG - chr8.2:2656308-2656327 None:intergenic 55.0%
! GAGGCTGGTCCAATACGCAT+GGG - chr8.2:2655477-2655496 None:intergenic 55.0%
! TGGTAACTTGCATACCCCGC+CGG - chr8.2:2658041-2658060 None:intergenic 55.0%
! TTGTTTCTCTCACACCGGCG+AGG + chr8.2:2654372-2654391 MS.gene62064:CDS 55.0%
!! TTGACTTCATCGGCGCTGGT+AGG - chr8.2:2654298-2654317 None:intergenic 55.0%
CGGGTTAAAAGGTGGGGTCC+TGG + chr8.2:2656306-2656325 MS.gene62064:intron 60.0%
GCTGTTGCTGCTGAGGAGGT+TGG - chr8.2:2658061-2658080 None:intergenic 60.0%
GGAACGGCCGATTGTTGAGG+AGG - chr8.2:2654271-2654290 None:intergenic 60.0%
GGAGGAACGGCCGATTGTTG+AGG - chr8.2:2654274-2654293 None:intergenic 60.0%
GGCCCACATCTGAGGAGGTT+GGG - chr8.2:2654236-2654255 None:intergenic 60.0%
GGCCGATTGTTGAGGAGGCT+GGG - chr8.2:2654266-2654285 None:intergenic 60.0%
GGGATCTTGTCCAGCAGGAG+GGG - chr8.2:2658000-2658019 None:intergenic 60.0%
GTGTTAGGGGCCCACATCTG+AGG - chr8.2:2654244-2654263 None:intergenic 60.0%
TGTATGGCAGCTATCCGGCG+GGG + chr8.2:2658024-2658043 MS.gene62064:CDS 60.0%
TTAGGGGCCCACATCTGAGG+AGG - chr8.2:2654241-2654260 None:intergenic 60.0%
! GACCCCACCTTTTAACCCGG+TGG - chr8.2:2656305-2656324 None:intergenic 60.0%
!! ACTTCATCGGCGCTGGTAGG+AGG - chr8.2:2654295-2654314 None:intergenic 60.0%
CGGCCGATTGTTGAGGAGGC+TGG - chr8.2:2654267-2654286 None:intergenic 65.0%
GCCGAAGCCCGGCAAACAAC+AGG + chr8.2:2657797-2657816 MS.gene62064:intron 65.0%
GCTTTCATGGGGCCGAAGCC+CGG + chr8.2:2657786-2657805 MS.gene62064:CDS 65.0%
GGCTTCGGCCCCATGAAAGC+CGG - chr8.2:2657786-2657805 None:intergenic 65.0%
GGGCCCACATCTGAGGAGGT+TGG - chr8.2:2654237-2654256 None:intergenic 65.0%
TCGTTCTGTGCCCCCCCTTG+TGG - chr8.2:2657373-2657392 None:intergenic 65.0%
!! GGCGCTGGTAGGAGGAGGAA+CGG - chr8.2:2654287-2654306 None:intergenic 65.0%
!! TCATCGGCGCTGGTAGGAGG+AGG - chr8.2:2654292-2654311 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 2654199 2658097 2654199 ID=MS.gene62064
chr8.2 mRNA 2654199 2658097 2654199 ID=MS.gene62064.t1;Parent=MS.gene62064
chr8.2 exon 2654199 2654393 2654199 ID=MS.gene62064.t1.exon1;Parent=MS.gene62064.t1
chr8.2 CDS 2654199 2654393 2654199 ID=cds.MS.gene62064.t1;Parent=MS.gene62064.t1
chr8.2 exon 2655060 2655181 2655060 ID=MS.gene62064.t1.exon2;Parent=MS.gene62064.t1
chr8.2 CDS 2655060 2655181 2655060 ID=cds.MS.gene62064.t1;Parent=MS.gene62064.t1
chr8.2 exon 2655228 2655318 2655228 ID=MS.gene62064.t1.exon3;Parent=MS.gene62064.t1
chr8.2 CDS 2655228 2655318 2655228 ID=cds.MS.gene62064.t1;Parent=MS.gene62064.t1
chr8.2 exon 2655348 2655528 2655348 ID=MS.gene62064.t1.exon4;Parent=MS.gene62064.t1
chr8.2 CDS 2655348 2655528 2655348 ID=cds.MS.gene62064.t1;Parent=MS.gene62064.t1
chr8.2 exon 2657356 2657414 2657356 ID=MS.gene62064.t1.exon5;Parent=MS.gene62064.t1
chr8.2 CDS 2657356 2657414 2657356 ID=cds.MS.gene62064.t1;Parent=MS.gene62064.t1
chr8.2 exon 2657488 2657618 2657488 ID=MS.gene62064.t1.exon6;Parent=MS.gene62064.t1
chr8.2 CDS 2657488 2657618 2657488 ID=cds.MS.gene62064.t1;Parent=MS.gene62064.t1
chr8.2 exon 2657723 2657807 2657723 ID=MS.gene62064.t1.exon7;Parent=MS.gene62064.t1
chr8.2 CDS 2657723 2657807 2657723 ID=cds.MS.gene62064.t1;Parent=MS.gene62064.t1
chr8.2 exon 2657915 2658097 2657915 ID=MS.gene62064.t1.exon8;Parent=MS.gene62064.t1
chr8.2 CDS 2657915 2658097 2657915 ID=cds.MS.gene62064.t1;Parent=MS.gene62064.t1
Gene Sequence

>MS.gene62064

ATGATGATGCATCCTCCTCAGCCTCAACCACAAACCCAACCTCCTCAGATGTGGGCCCCTAACACCCAGCCTCCTCAACAATCGGCCGTTCCTCCTCCTACCAGCGCCGATGAAGTCAAAACTCTCTGGATCGGTGATTTGCAATATTGGATGGACGAGAATTATCTTTATAATTGTTTCTCTCACACCGGCGAGGTATAATAACCGCTTTTATTATCATATCTTTTTAGATCTATAGATTTTAAATCTCTTAGGGTTAGTTTTTTTTTTTTTTGTAGAAAAATTGTTAGGGTTAGTTGATGCATGAAGTTTATGTTAATCGGATCAAAAACAATTTTGTTATGTTATTGTTTAATTTGAATACGAGGTTCTTTTATAAAGACCAGTTTGTTATGAAAACGATGTTTTCTGTTTTGAGAAATTGGATAATCTTTTATTCATTGGATTTAATTTGTTTACACGGACGGTGTAAAACTTTTGTACACATGCATGCTATCAAATCATGATACGGGGTTCTCTAAGTTGTTTATGAATCGATGTATGGTTTATTTGTTGGATTTAATTTGCATACACAGACAGTGCAAAGTTTTTTACACATGCATCCTAACAAATCACGACAATAGATATTTATGTAGATATTTTAGATTAGAAACCAAGCTATATATGGCACTATGGTGTTGATTTGTTTTACACTGTCAGTGCATATAGATTTATCTATAATTTATTCTGTATACTTGAACTGTTTATTATTCAAGGTGAAATTGTTATAGTATTATTTTCACTATATTGATAGTGAAATTATTATGTCAATTTTGAAGGTGTTTTTGTTTATATTTAAGGAGTTGTTCTGTTTCTTTGTAGGTTGGATCTGTTAAAGTTATTCGCAATAAGCTGAATAATCAATCTGAAGGTTATGGGTTTCTTGAGTTTATTAGCCGTGCTGGTGCTGAGAGAGTACTTCAAACATATAATGGCACCATTATGCCAAATGGTGGCCAGAATTTCAGATTGAACTGGGCCAACTTTTAGTTCTGGTGAGAAGCGCCATGTTGATTCTCCGGATTACACCATATTTGTTGGTGACTTGGCTGCTGATGTTAGTGATCATCATTTGACGGAAGTGTTTAGAACTCGTATAATTCAGTTAAGGGTGCCAAAGTTGTAATTGACAGGAACACTGGTAGGTCAAAAGGTTATGGTTTTGTTAGGTTTGCTGATGAGAGTGAACAAATGAGGGCGATGACTGAAATGCAAGGGGTTCTTTGTTCCACAAGACCCATGCGTATTGGACCAGCCTCTAACAAAAACCTTGGCACACAGACTTCAAAAGGTTGTTTTTTTAATGGTTGATATACATTGGCCAACATAAAGAAAAACTTGTGCTGTTTTCCTTCAGATGCCAGTGTTTAACCTATTGATGAATAATGATACTGTTTGGATATAATGCTTATGTCTCCCTGTTATCTTTTATCGTGTGAATCCCTAAATGTTATCCTTCAGTATAAGATAAGTTTTCAGTCAGGCTCTTGAAGATATGCTGTCTAAACTCGATGCTTGTTCGTAATTTCGTTTCAAAATTTGGGCTTAGTAAATCATGTAGAATAATAAAATCTTATAGAACTCGTGTTGTCTCAAAGCAGGGTTTCAAGAGGCTCTATCATCTCGAGCCTGGAACTGATTTCAGCTGAAATGGTTACCATTTTAGTGCATGCCTTCCGTAGGTCAGTAATCCGTTTATGTCTCGCCCATAATTTTTGGTGGCATTTTCTTTCCCGCCTTTATTCCGCACCTATTCTGTTTACTTTCTCTTTTCCTTTCGTTTTGTTGGCTTGCTTTTAAGCCAAATTACATACAGCAGCTATTCATAGTTTTTAATTTTTTTTCAGCCCACCTTTCTTTGTTTCAGCTCTTAATTTTTATTCTTTCGAGCTTTACTTCTTTTGGTATTTTAAATTATATCCAAATAATCTTAGTTCTTGCATTAGAACTACATCTTTCAATTTACTTGCAAGATTTATCTTATTCTACAGCAAATCTTGTTTACTTTAATAATTTTGGTTCATCTGTCTGATGCTTTAATAATTTAAGTTACCAATCTCAAATCCACCGGGTTAAAAGGTGGGGTCCTGGTCTTTAGTGCTTTCGTTGTGTAAAATCTGTACTATGCAGATTGGTTGTTCGTTGTCACGTTCTTTACTATTTATTTTCATGATGTTATGGCTTTTTCTTTCTTGGTTAGTTTCTGCACTGTTTGGCGTACTAAATTTATAATTTAGCATATATTGCTCAGTCTAATGGGTAGTGTAGTCTTTCGGAATTTCTAGTTGATGCGTTGTGAGTTTTTTCTGCACAAGCATTTGACAGTTTCCTGCTCTGTTCTTTATTTCAATCTCTACGTTAACTTTGATTATAATATAGGACATCAATCTCTATTGAACTTTGATTATAACAAGGAAGATCGATTCCATTTCTATATGCTTCGATGCATATGTAAACAAATTTACATACGCGCGCGCGCAGTCCTTGCATTTCTCAACACTTTAACTATTTTTATTGAATATTTAGGACACAGGAAGATCAATGCCATTTCTATATGCATATGTAAATAAATTTACACACACACAGACGTGCGCGCAGTCGTTGCATTGCTCAACACTTCAACTATTTTTATTGAATATTCATGTAATTGTTTGAATCGATGGTTTATTTTTGTCCATGATTTAATGTATAGATGAATAGTTATCTTCTCTTATATTTCAACAAGGATGGTGTGTGTAAGGCACCGACCTTTTTATTGCTCCTCAACCCTGGGTGCATCATTCATTGCACATGTTAAGTAAATTGAATGTGTTTATTTTATACTGACTTAATTTCTGTTTGTTGAATGTTTATAGTCATTTGTTGCTTCTGAAATTCCTCGTGTGCTTGCTTTTGGAATGAATTATCTTCATCTGTAAATGAATATTCGAAGCATCTGAATAGCTGTTTTGACTGAAAATATGAACAAATTTTGCTTGAAAATTATGTCACAGAAATACAAGCCGTGCATTTTATTATATTATGCTATTGGTTTATTTTCTGTCTTTTTTCTATGAACTGCTTCCTTCCATGTTCTATGTCATTTATCTTTTTATTTTATTTTACTTTGAAAATGCAGCTTCTTATCAGAATCCACAAGGGGGGGCACAGAACGAGAATGATCCAAATAATACAACTGTGCGTATTTCTTTATAACATAGAAATTGTTCTCTTTTATACTTGGTTGTAACCTGATTTCTCTTTGTTGCAGATTTTTGTTGGCAATTTGGATCCTAATGTCACAGATGAACATTTGAAACAAGTTTTTACTCAGTATGGTGAACTGGTTCATGTTAAGATTCCATCAGGCAAGAGATGTGGGTTTGTCCAATTTGCAGACAGGTAAATTAATCATTTAGATATTTGATTTGATATTTATCTTATGACGGTATTACTTCCCCATTCTATTCAGTGCTCTGATTGTGCCACTATATTTAATATATCAGGAGTTCTGCTGAGGAAGCACTACGGGTTTTAAATGGTACGCTTCTAGGTGGACAAAATGTCCGGCTTTCATGGGGCCGAAGCCCGGCAAACAACAGGTAATAGTTTTGAGATTGTTGTAAACCTGAGCTGCATTTGATGCAAACATTTTTGACTTATTTTGATCAATAATGGATTTCCTTGCTGATTTTCAGACCCAACAAGATCCAAACCAGTGGAATGGTAGTAGTGGTTACTTCGGCGGATATGCCCAAGGTTATGAAAATTATGCTTATGCCCCTCCTGCTGGACAAGATCCCAATATGTATGGCAGCTATCCGGCGGGGTATGCAAGTTACCAACCTCCTCAGCAGCAACAGCAATTAGGATATAGTTAA
Protein sequence

>MS.gene62064.t1

MMMHPPQPQPQTQPPQMWAPNTQPPQQSAVPPPTSADEVKTLWIGDLQYWMDENYLYNCFSHTGEVGSVKVIRNKLNNQSEGYGFLEFISRAGAERVLQTYNGTIISGEKRHVDSPDYTIFVGDLAADVSDHHLTEGAKVVIDRNTGRSKGYGFVRFADESEQMRAMTEMQGVLCSTRPMRIGPASNKNLGTQTSKASYQNPQGGAQNENDPNNTTIFVGNLDPNVTDEHLKQVFTQYGELVHVKIPSGKRCGFVQFADRSSAEEALRVLNGTLLGGQNVRLSWGRSPTQQDPNQWNGSSGYFGGYAQGYENYAYAPPAGQDPNMYGSYPAGYASYQPPQQQQQLGYS