AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene040118

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040118.t1 MTR_8g107270 94.040 302 18 0 9 310 16 317 0.0 595
MS.gene040118.t1 MTR_4g078545 59.233 287 117 0 10 296 74 360 4.16e-124 360
MS.gene040118.t1 MTR_3g028590 58.681 288 119 0 7 294 93 380 2.18e-122 356
MS.gene040118.t1 MTR_3g028590 58.681 288 119 0 7 294 89 376 3.47e-122 356
MS.gene040118.t1 MTR_8g036880 58.904 292 119 1 6 296 97 388 3.80e-119 348
MS.gene040118.t1 MTR_2g098070 59.170 289 118 0 6 294 61 349 5.76e-117 342
MS.gene040118.t1 MTR_3g104500 36.054 294 181 5 6 295 29 319 4.34e-60 195
MS.gene040118.t1 MTR_8g027975 29.392 296 174 9 6 276 46 331 9.58e-32 121
MS.gene040118.t1 MTR_5g009500 29.373 303 163 10 6 276 33 316 1.25e-31 121
MS.gene040118.t1 MTR_5g009500 25.616 203 134 4 14 205 144 340 1.06e-11 65.1
MS.gene040118.t1 MTR_3g104600 32.881 295 155 12 7 276 31 307 2.43e-31 120
MS.gene040118.t1 MTR_6g091800 27.129 317 191 11 7 301 15 313 1.12e-27 109
MS.gene040118.t1 MTR_4g016730 28.571 280 170 8 22 296 100 354 4.42e-27 109
MS.gene040118.t1 MTR_8g096050 27.562 283 185 6 8 286 224 490 7.97e-27 110
MS.gene040118.t1 MTR_7g068340 28.520 277 177 9 22 296 98 355 2.40e-26 107
MS.gene040118.t1 MTR_7g064980 27.687 307 197 9 10 302 24 319 2.73e-26 106
MS.gene040118.t1 MTR_6g091800 26.686 341 186 12 7 301 15 337 7.44e-25 102
MS.gene040118.t1 MTR_6g091800 29.200 250 150 7 7 244 15 249 9.16e-25 100
MS.gene040118.t1 MTR_6g091800 26.909 275 166 9 7 264 15 271 1.84e-24 100
MS.gene040118.t1 MTR_6g091800 27.244 312 167 10 7 276 15 308 2.04e-24 100
MS.gene040118.t1 MTR_1g012030 27.152 302 190 11 2 296 131 409 6.40e-24 101
MS.gene040118.t1 MTR_1g012030 28.079 203 123 6 6 204 228 411 1.32e-15 77.4
MS.gene040118.t1 MTR_3g098460 26.042 288 193 6 3 286 197 468 6.76e-24 101
MS.gene040118.t1 MTR_3g107510 27.240 279 175 9 14 286 125 381 9.04e-24 100
MS.gene040118.t1 MTR_3g107510 27.670 206 120 6 6 204 211 394 2.70e-15 76.3
MS.gene040118.t1 MTR_7g084400 27.046 281 180 7 11 286 213 473 1.95e-23 100
MS.gene040118.t1 MTR_6g091800 28.102 274 146 7 7 244 15 273 7.62e-22 93.6
MS.gene040118.t1 MTR_6g091800 26.087 299 162 9 7 264 15 295 1.43e-21 92.8
MS.gene040118.t1 MTR_8g090210 28.387 310 164 10 6 286 105 385 1.78e-21 94.0
MS.gene040118.t1 MTR_3g074490 27.393 303 172 9 8 286 113 391 6.39e-21 92.4
MS.gene040118.t1 MTR_4g114320 24.915 293 195 10 9 296 121 393 1.18e-19 89.0
MS.gene040118.t1 MTR_2g436930 23.453 307 200 7 14 291 18 318 1.51e-19 87.8
MS.gene040118.t1 MTR_5g081150 25.614 285 196 6 10 286 33 309 4.02e-19 86.7
MS.gene040118.t1 MTR_4g119770 22.256 328 215 7 1 294 1 322 7.21e-18 83.2
MS.gene040118.t1 MTR_6g091800 30.220 182 103 6 7 177 15 183 7.38e-18 80.5
MS.gene040118.t1 MTR_6g092210 25.000 260 176 7 54 301 1 253 1.11e-16 78.6
MS.gene040118.t1 MTR_6g034145 24.535 269 172 8 54 301 1 259 3.13e-16 77.4
MS.gene040118.t1 MTR_7g084400 29.688 192 117 5 11 200 207 382 1.02e-15 77.4
MS.gene040118.t1 MTR_7g109135 23.469 294 209 10 14 300 25 309 1.62e-15 76.3
MS.gene040118.t1 MTR_4g119770 23.282 262 172 5 1 239 1 256 4.50e-15 73.9
MS.gene040118.t1 MTR_6g091800 29.126 206 98 7 7 177 15 207 6.70e-15 72.8
MS.gene040118.t1 MTR_4g005330 21.333 300 192 5 9 286 64 341 1.96e-13 70.5
MS.gene040118.t1 MTR_5g081150 26.667 195 131 3 10 200 33 219 4.43e-13 68.6
MS.gene040118.t1 MTR_5g081150 26.667 195 131 3 10 200 33 219 4.66e-13 68.2
MS.gene040118.t1 MTR_6g091910 25.373 201 133 4 7 200 15 205 7.33e-13 67.0
MS.gene040118.t1 MTR_4g057850 23.051 295 196 11 12 293 23 299 7.36e-13 68.6
MS.gene040118.t1 MTR_4g005330 21.356 295 188 5 14 286 3 275 3.08e-12 66.2
MS.gene040118.t1 MTR_7g109135 22.973 296 201 10 14 300 25 302 4.38e-12 66.2
MS.gene040118.t1 MTR_5g030530 24.211 285 187 8 8 277 5 275 2.39e-11 63.9
MS.gene040118.t1 MTR_1g097890 22.295 305 188 9 12 308 52 315 3.21e-11 63.5
MS.gene040118.t1 MTR_5g032420 21.502 293 198 6 9 272 4 293 4.62e-11 63.2
MS.gene040118.t1 MTR_7g066690 20.803 274 206 6 6 276 12 277 6.47e-11 62.4
MS.gene040118.t1 MTR_3g462140 21.575 292 183 6 6 276 10 276 6.75e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040118.t1 AT5G17400 75.839 298 72 0 3 300 6 303 2.69e-164 459
MS.gene040118.t1 AT4G28390 60.000 290 116 0 7 296 79 368 1.35e-123 359
MS.gene040118.t1 AT4G28390 60.000 290 116 0 7 296 79 368 1.35e-123 359
MS.gene040118.t1 AT5G13490 58.219 292 121 1 6 296 83 374 5.46e-119 347
MS.gene040118.t1 AT5G13490 58.219 292 121 1 6 296 83 374 5.46e-119 347
MS.gene040118.t1 AT3G08580 57.192 292 124 1 6 296 79 370 7.69e-117 342
MS.gene040118.t1 AT3G08580 57.192 292 124 1 6 296 79 370 7.69e-117 342
MS.gene040118.t1 AT5G56450 37.667 300 170 8 7 295 30 323 1.45e-58 191
MS.gene040118.t1 AT2G37890 29.966 297 191 7 9 296 43 331 3.25e-33 125
MS.gene040118.t1 AT4G26180 31.469 286 169 9 7 278 17 289 3.29e-33 124
MS.gene040118.t1 AT4G26180 31.469 286 169 9 7 278 17 289 8.41e-33 124
MS.gene040118.t1 AT4G01100 29.412 306 163 8 5 276 36 322 1.18e-30 118
MS.gene040118.t1 AT1G14560 33.574 277 146 13 22 276 38 298 3.72e-29 114
MS.gene040118.t1 AT5G01500 29.603 277 174 10 22 296 130 387 3.81e-29 115
MS.gene040118.t1 AT3G51870 27.517 298 186 9 22 307 102 381 5.54e-29 114
MS.gene040118.t1 AT1G14560 33.574 277 146 13 22 276 38 298 7.01e-29 113
MS.gene040118.t1 AT4G01100 30.063 316 162 10 5 276 36 336 1.80e-28 113
MS.gene040118.t1 AT4G01100 29.210 291 153 8 20 276 1 272 4.02e-28 110
MS.gene040118.t1 AT3G53940 27.687 307 197 8 9 302 71 365 6.02e-28 111
MS.gene040118.t1 AT3G53940 25.366 205 135 4 6 207 175 364 5.43e-11 63.2
MS.gene040118.t1 AT3G55640 27.397 292 186 9 8 286 35 313 1.67e-27 109
MS.gene040118.t1 AT3G55640 27.397 292 186 9 8 286 35 313 1.67e-27 109
MS.gene040118.t1 AT3G21390 25.714 315 193 7 10 291 18 324 7.31e-24 100
MS.gene040118.t1 AT3G21390 25.879 313 195 6 10 291 40 346 8.61e-24 100
MS.gene040118.t1 AT5G61810 28.571 273 167 7 8 276 62 310 1.01e-23 99.8
MS.gene040118.t1 AT5G61810 27.915 283 176 7 8 286 205 463 2.49e-23 100
MS.gene040118.t1 AT5G07320 26.740 273 168 8 20 286 218 464 6.62e-23 99.0
MS.gene040118.t1 AT5G51050 26.071 280 184 5 11 286 223 483 8.94e-23 98.6
MS.gene040118.t1 AT3G55640 29.208 202 121 6 8 200 35 223 1.82e-22 94.7
MS.gene040118.t1 AT3G55640 29.208 202 121 6 8 200 35 223 1.82e-22 94.7
MS.gene040118.t1 AT5G64970 28.146 302 171 10 8 286 132 410 8.91e-21 92.4
MS.gene040118.t1 AT5G64970 28.571 294 164 10 8 278 132 402 1.75e-20 91.3
MS.gene040118.t1 AT5G48970 24.924 329 195 11 10 297 21 338 2.64e-19 87.4
MS.gene040118.t1 AT3G53940 28.500 200 123 4 9 200 71 258 2.68e-19 86.7
MS.gene040118.t1 AT5G64970 28.053 303 171 11 8 286 7 286 3.38e-19 86.7
MS.gene040118.t1 AT3G20240 22.330 309 180 7 8 286 49 327 3.59e-19 87.0
MS.gene040118.t1 AT4G32400 25.347 288 179 10 9 286 112 373 1.19e-18 85.9
MS.gene040118.t1 AT1G78180 28.620 297 164 11 14 286 131 403 1.98e-16 79.7
MS.gene040118.t1 AT1G14560 32.418 182 102 7 115 278 24 202 1.71e-15 74.7
MS.gene040118.t1 AT3G54110 24.643 280 188 9 6 276 10 275 1.04e-14 73.9
MS.gene040118.t1 AT5G48970 24.265 272 163 8 59 297 6 267 4.19e-14 71.6
MS.gene040118.t1 AT5G58970 22.517 302 224 7 6 304 11 305 1.38e-13 70.5
MS.gene040118.t1 AT5G66380 27.368 190 132 4 111 296 5 192 1.14e-12 67.8
MS.gene040118.t1 AT5G01340 26.738 187 124 3 15 197 116 293 4.75e-12 65.9
MS.gene040118.t1 AT1G79900 25.664 226 142 6 56 273 51 258 2.22e-11 63.9
MS.gene040118.t1 AT1G34065 22.295 305 185 9 9 302 73 336 7.80e-11 62.8
MS.gene040118.t1 AT1G34065 22.295 305 185 9 9 302 80 343 8.34e-11 62.4

Find 77 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGGGAGCTGGGGTGCTTGC+TGG 0.170497 4.4:+306503 MS.gene040118:CDS
TTGCATTATAATACTTATTC+TGG 0.231839 4.4:-306397 None:intergenic
GGGATTCGGTGTTTCAATAT+TGG 0.231853 4.4:+305791 MS.gene040118:CDS
AAGAGGGTCTCCTTGCCTTT+TGG 0.234640 4.4:+305096 MS.gene040118:CDS
GGTTACTTCAAAAGCATCTT+TGG 0.242168 4.4:+305540 MS.gene040118:CDS
CTTCTAGGGATGGCGGGAGC+TGG 0.249873 4.4:+306491 MS.gene040118:CDS
AATTGGTGCCACTGCAGTTT+TGG 0.258307 4.4:-304967 None:intergenic
ACTGGTCACCGTCAGTTTAA+AGG 0.285006 4.4:+305711 MS.gene040118:CDS
TCATTTATTTCATGACTCTT+TGG 0.289721 4.4:-306580 None:intergenic
TTCTTTGCTAGTTTCTTGTT+AGG 0.299649 4.4:+306293 MS.gene040118:CDS
TTCTAGGGATGGCGGGAGCT+GGG 0.318989 4.4:+306492 MS.gene040118:CDS
GGATTCGGTGTTTCAATATT+GGG 0.326349 4.4:+305792 MS.gene040118:CDS
GTCACTGCAAACATGCTTCT+AGG 0.326966 4.4:+306476 MS.gene040118:CDS
TTGCTAGTTTCTTGTTAGGT+TGG 0.348330 4.4:+306297 MS.gene040118:CDS
AACTAAAACAATAGGCTTCA+TGG 0.352724 4.4:-305856 None:intergenic
GGGTACATCAAGTGGTTTGC+TGG 0.371317 4.4:+305582 MS.gene040118:CDS
AAAAGCTTCACTCTTTCAAT+TGG 0.371863 4.4:-304984 None:intergenic
TTTGCTGGGAACGTGGCTTC+AGG 0.391111 4.4:+305597 MS.gene040118:CDS
TGGAGCATCACAACTGTATC+TGG 0.393342 4.4:+306317 MS.gene040118:CDS
CGGGAGCTGGGGTGCTTGCT+GGG 0.401594 4.4:+306504 MS.gene040118:CDS
AAAGGTCCAACTAAAACAAT+AGG 0.409592 4.4:-305864 None:intergenic
GGACTTTGCACGTACAAGAT+TGG 0.415555 4.4:+305662 MS.gene040118:CDS
ATGGTGTTGCTGGCTTGTAC+AGG 0.427812 4.4:+305769 MS.gene040118:CDS
ATATCAAGTGATGGTGTTGC+TGG 0.431324 4.4:+305759 MS.gene040118:CDS
TGTATCGAGGAATGTACTTT+GGG 0.438762 4.4:+305823 MS.gene040118:CDS
GATGTACCCATCTTTCTCTC+TGG 0.444325 4.4:-305568 None:intergenic
GTATAAATGCTTACCTCAAA+AGG 0.445098 4.4:-305882 None:intergenic
GCTGGCTTGTACAGGGGATT+CGG 0.454958 4.4:+305777 MS.gene040118:CDS
CATGCATTCCGTGAGATTGT+TGG 0.459314 4.4:+306422 MS.gene040118:CDS
GTGGCTTCAGGCAGTGCTGC+GGG 0.472041 4.4:+305609 MS.gene040118:CDS
CGCCGGCGTAATGTGTCAAA+AGG 0.486557 4.4:-306353 None:intergenic
CGTGAGATTGTTGGACAAGA+GGG 0.488183 4.4:+306431 MS.gene040118:CDS
ATGTATCGAGGAATGTACTT+TGG 0.489826 4.4:+305822 MS.gene040118:CDS
TAAAAGAGTCTTTGCTGAAG+AGG 0.493467 4.4:+305079 MS.gene040118:CDS
ATAATACTTATTCTGGTGCC+CGG 0.509415 4.4:-306390 None:intergenic
TACTGTGTCTAGATGAGATA+CGG 0.509802 4.4:-306541 None:intergenic
AAAAGAGTCTTTGCTGAAGA+GGG 0.517416 4.4:+305080 MS.gene040118:CDS
TCTAGGGATGGCGGGAGCTG+GGG 0.518411 4.4:+306493 MS.gene040118:CDS
CGGCGTATGATGCTAACCTC+CGG 0.521609 4.4:+306371 MS.gene040118:CDS
GGATATTCCAGAGAGAAAGA+TGG 0.522046 4.4:+305561 MS.gene040118:CDS
GGTGTTGCTGGCTTGTACAG+GGG 0.525521 4.4:+305771 MS.gene040118:CDS
AATATCTAATAACATTGGCC+TGG 0.525661 4.4:-305127 None:intergenic
TGGTGTTGCTGGCTTGTACA+GGG 0.527675 4.4:+305770 MS.gene040118:CDS
GGTGACCAGTGGCATGGCAC+TGG 0.528489 4.4:-305698 None:intergenic
GGTTTCTTAGCTCTATTTCG+AGG 0.528923 4.4:+306452 MS.gene040118:CDS
CAAAGCGAAATGATTAAAAG+AGG 0.529225 4.4:+305017 MS.gene040118:CDS
TCACTGCAAACATGCTTCTA+GGG 0.533524 4.4:+306477 MS.gene040118:CDS
GGCCTGGTGACCTCTCCAAA+AGG 0.537477 4.4:-305111 None:intergenic
TCAGTCCAGTGCCATGCCAC+TGG 0.539129 4.4:+305693 MS.gene040118:CDS
GATATTCCAGAGAGAAAGAT+GGG 0.540943 4.4:+305562 MS.gene040118:CDS
CCGTGAGATTGTTGGACAAG+AGG 0.542206 4.4:+306430 MS.gene040118:CDS
GGCGTATGATGCTAACCTCC+GGG 0.550555 4.4:+306372 MS.gene040118:CDS
CGTGGCTTCAGGCAGTGCTG+CGG 0.550723 4.4:+305608 MS.gene040118:CDS
AACATGCTTCTAGGGATGGC+GGG 0.552983 4.4:+306485 MS.gene040118:CDS
AAAGTACATTCCTCGATACA+TGG 0.553101 4.4:-305820 None:intergenic
TAAAGGTATTAGACAAACCA+AGG 0.560990 4.4:-305058 None:intergenic
CAAATGATACAAAAGTATTG+AGG 0.568033 4.4:-305640 None:intergenic
TGCAAACATGCTTCTAGGGA+TGG 0.571454 4.4:+306481 MS.gene040118:CDS
CGTAAAACAATATCAAGTGA+TGG 0.574072 4.4:+305750 MS.gene040118:CDS
GGTACATCAAGTGGTTTGCT+GGG 0.575345 4.4:+305583 MS.gene040118:CDS
AAGAATCACCTCACCTGTGT+GGG 0.577980 4.4:-305152 None:intergenic
GGGAAAATATCTAATAACAT+TGG 0.589711 4.4:-305132 None:intergenic
CAAGTGGTTTGCTGGGAACG+TGG 0.595268 4.4:+305590 MS.gene040118:CDS
GTATTAGACAAACCAAGGTA+TGG 0.595810 4.4:-305053 None:intergenic
AGAAAGATGGGTACATCAAG+TGG 0.605042 4.4:+305574 MS.gene040118:CDS
GAATTAGTCCTTTAAACTGA+CGG 0.606819 4.4:-305719 None:intergenic
CCTCTTGTCCAACAATCTCA+CGG 0.613033 4.4:-306430 None:intergenic
GGTGACCTCTCCAAAAGGCA+AGG 0.614061 4.4:-305106 None:intergenic
AAACATGCTTCTAGGGATGG+CGG 0.631697 4.4:+306484 MS.gene040118:CDS
ACTGACGGTGACCAGTGGCA+TGG 0.636991 4.4:-305704 None:intergenic
AAAGAATCACCTCACCTGTG+TGG 0.643453 4.4:-305153 None:intergenic
TTGGGAATCACCATGTATCG+AGG 0.659477 4.4:+305810 MS.gene040118:CDS
AATCATATCCAAAACTGCAG+TGG 0.672395 4.4:+304959 MS.gene040118:CDS
TTTAAACTGACGGTGACCAG+TGG 0.674236 4.4:-305709 None:intergenic
ATGGCACTGGACTGAATCAG+TGG 0.678456 4.4:-305685 None:intergenic
ATACTTATTCTGGTGCCCGG+AGG 0.698949 4.4:-306387 None:intergenic
CGGAGGTTAGCATCATACGC+CGG 0.703548 4.4:-306370 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATAAAAACTCTAATAT+TGG - chr4.4:306024-306043 None:intergenic 10.0%
!! AAGATGTATGTTAAAATTAA+AGG - chr4.4:306076-306095 None:intergenic 15.0%
!! TATGTTAAAATTAAAGGTAA+GGG - chr4.4:306070-306089 None:intergenic 15.0%
!! TTAAATTGAGAATTATTTGA+GGG - chr4.4:305355-305374 None:intergenic 15.0%
!! TTTAAATTGAGAATTATTTG+AGG - chr4.4:305356-305375 None:intergenic 15.0%
!! CATTACAAAACAAGTATAAT+AGG - chr4.4:305220-305239 None:intergenic 20.0%
!! GTATGTTAAAATTAAAGGTA+AGG - chr4.4:306071-306090 None:intergenic 20.0%
!! TTGCATTATAATACTTATTC+TGG - chr4.4:306400-306419 None:intergenic 20.0%
!! TTTGATTGGAATACAAAATA+CGG - chr4.4:305428-305447 None:intergenic 20.0%
!!! AAAATCAGAATCATTTTCAA+AGG + chr4.4:304911-304930 MS.gene040118:CDS 20.0%
!!! ATTTTCAAAGGATTTTGTAA+TGG + chr4.4:304923-304942 MS.gene040118:CDS 20.0%
!!! TACTTGTTTTGTAATGTTTT+TGG + chr4.4:305224-305243 MS.gene040118:intron 20.0%
!!! TTTTCAAAGGATTTTGTAAT+GGG + chr4.4:304924-304943 MS.gene040118:CDS 20.0%
! AACATAGATCTATAATTCAC+AGG + chr4.4:305922-305941 MS.gene040118:intron 25.0%
! GGGAAAATATCTAATAACAT+TGG - chr4.4:305135-305154 None:intergenic 25.0%
! TATTTGAGCAAACAGTTAAT+TGG + chr4.4:305489-305508 MS.gene040118:intron 25.0%
! TTAGTGAACTAATTACAACA+CGG - chr4.4:305284-305303 None:intergenic 25.0%
! TTATTCATCACATTACTATG+AGG + chr4.4:305177-305196 MS.gene040118:intron 25.0%
! TTGATTGGAATACAAAATAC+GGG - chr4.4:305427-305446 None:intergenic 25.0%
!! CTCAATACTTTTGTATCATT+TGG + chr4.4:305641-305660 MS.gene040118:CDS 25.0%
!! TATTATGCTGCTTATATTGA+TGG + chr4.4:306039-306058 MS.gene040118:intron 25.0%
!!! CAAATGATACAAAAGTATTG+AGG - chr4.4:305643-305662 None:intergenic 25.0%
!!! CTTTTGATTTATATTCTGCA+GGG + chr4.4:306266-306285 MS.gene040118:intron 25.0%
!!! TCTTTTGATTTATATTCTGC+AGG + chr4.4:306265-306284 MS.gene040118:intron 25.0%
!!! TTTTGATTTATATTCTGCAG+GGG + chr4.4:306267-306286 MS.gene040118:intron 25.0%
AAAAGCTTCACTCTTTCAAT+TGG - chr4.4:304987-305006 None:intergenic 30.0%
AAAGGTCCAACTAAAACAAT+AGG - chr4.4:305867-305886 None:intergenic 30.0%
AACTAAAACAATAGGCTTCA+TGG - chr4.4:305859-305878 None:intergenic 30.0%
AACTAAACTAATGTCCATAG+AGG - chr4.4:305329-305348 None:intergenic 30.0%
AATATCTAATAACATTGGCC+TGG - chr4.4:305130-305149 None:intergenic 30.0%
ATCAAAATGCTATACCAACA+AGG - chr4.4:306189-306208 None:intergenic 30.0%
ATCTATAATTCACAGGCAAT+GGG + chr4.4:305929-305948 MS.gene040118:intron 30.0%
ATGAATTTATGTCAGAGCAT+TGG + chr4.4:306207-306226 MS.gene040118:intron 30.0%
ATTAGTTCACTAAAAAGTCC+AGG + chr4.4:305291-305310 MS.gene040118:intron 30.0%
CAAAGCGAAATGATTAAAAG+AGG + chr4.4:305017-305036 MS.gene040118:CDS 30.0%
CAAGTTAGACAACAGTAATT+TGG + chr4.4:306114-306133 MS.gene040118:intron 30.0%
CGTAAAACAATATCAAGTGA+TGG + chr4.4:305750-305769 MS.gene040118:CDS 30.0%
GAATTAGTCCTTTAAACTGA+CGG - chr4.4:305722-305741 None:intergenic 30.0%
GTATAAATGCTTACCTCAAA+AGG - chr4.4:305885-305904 None:intergenic 30.0%
TAGTGAACTAATTACAACAC+GGG - chr4.4:305283-305302 None:intergenic 30.0%
TCAAAATGCTATACCAACAA+GGG - chr4.4:306188-306207 None:intergenic 30.0%
! CAAACAGTTAATTGGTCTAT+AGG + chr4.4:305497-305516 MS.gene040118:intron 30.0%
! CTCTTTTAATCATTTCGCTT+TGG - chr4.4:305019-305038 None:intergenic 30.0%
! GCTTTCAATTTTGCATTCAA+AGG + chr4.4:305519-305538 MS.gene040118:CDS 30.0%
! TATTAGATATTTTCCCACAC+AGG + chr4.4:305139-305158 MS.gene040118:CDS 30.0%
! TTCAGCAAAGACTCTTTTAA+AGG - chr4.4:305078-305097 None:intergenic 30.0%
! TTCTTTGCTAGTTTCTTGTT+AGG + chr4.4:306293-306312 MS.gene040118:CDS 30.0%
!! TAAAGGTATTAGACAAACCA+AGG - chr4.4:305061-305080 None:intergenic 30.0%
!!! ATGAAGCCTATTGTTTTAGT+TGG + chr4.4:305858-305877 MS.gene040118:CDS 30.0%
AAAAGAGTCTTTGCTGAAGA+GGG + chr4.4:305080-305099 MS.gene040118:CDS 35.0%
AAAGTACATTCCTCGATACA+TGG - chr4.4:305823-305842 None:intergenic 35.0%
AACCTAAAAACACCATACCT+TGG + chr4.4:305041-305060 MS.gene040118:CDS 35.0%
AAGACTCATATTCCAATGCA+AGG - chr4.4:306249-306268 None:intergenic 35.0%
AATCATATCCAAAACTGCAG+TGG + chr4.4:304959-304978 MS.gene040118:CDS 35.0%
AGACTCATATTCCAATGCAA+GGG - chr4.4:306248-306267 None:intergenic 35.0%
ATAATACTTATTCTGGTGCC+CGG - chr4.4:306393-306412 None:intergenic 35.0%
ATGTATCGAGGAATGTACTT+TGG + chr4.4:305822-305841 MS.gene040118:CDS 35.0%
CATTACTATGAGGTGGTAAA+TGG + chr4.4:305187-305206 MS.gene040118:intron 35.0%
GATATTCCAGAGAGAAAGAT+GGG + chr4.4:305562-305581 MS.gene040118:CDS 35.0%
GATCTATAATTCACAGGCAA+TGG + chr4.4:305928-305947 MS.gene040118:intron 35.0%
GGGTTAATAATTGCTTCTCT+TGG + chr4.4:305960-305979 MS.gene040118:intron 35.0%
GTATTAGACAAACCAAGGTA+TGG - chr4.4:305056-305075 None:intergenic 35.0%
TAAAAGAGTCTTTGCTGAAG+AGG + chr4.4:305079-305098 MS.gene040118:CDS 35.0%
TACTGTGTCTAGATGAGATA+CGG - chr4.4:306544-306563 None:intergenic 35.0%
TGTATCGAGGAATGTACTTT+GGG + chr4.4:305823-305842 MS.gene040118:CDS 35.0%
TTCATCACATTACTATGAGG+TGG + chr4.4:305180-305199 MS.gene040118:intron 35.0%
! AAAGACTGTTATTTGAGAGC+AGG - chr4.4:305452-305471 None:intergenic 35.0%
! AACCAAGGTATGGTGTTTTT+AGG - chr4.4:305046-305065 None:intergenic 35.0%
! AAGACTGTTATTTGAGAGCA+GGG - chr4.4:305451-305470 None:intergenic 35.0%
! TTGCTAGTTTCTTGTTAGGT+TGG + chr4.4:306297-306316 MS.gene040118:CDS 35.0%
! TTTTTCGTCTGTTCCATGTT+AGG + chr4.4:305990-306009 MS.gene040118:intron 35.0%
!! GGATTCGGTGTTTCAATATT+GGG + chr4.4:305792-305811 MS.gene040118:CDS 35.0%
!! GGTTACTTCAAAAGCATCTT+TGG + chr4.4:305540-305559 MS.gene040118:CDS 35.0%
!! TCAAAGGATTTTGTAATGGG+AGG + chr4.4:304927-304946 MS.gene040118:CDS 35.0%
!!! GTTTATTTTTGCCCTTGCAT+TGG + chr4.4:306234-306253 MS.gene040118:intron 35.0%
!!! TGTTTTAGTTGGACCTTTTG+AGG + chr4.4:305869-305888 MS.gene040118:CDS 35.0%
ACAAGTGACAATGAACACAC+AGG - chr4.4:305392-305411 None:intergenic 40.0%
ACAGGCAATGGGAAAATATG+GGG + chr4.4:305940-305959 MS.gene040118:intron 40.0%
AGAAAGATGGGTACATCAAG+TGG + chr4.4:305574-305593 MS.gene040118:CDS 40.0%
ATACCAACAAGGGATGTATC+TGG - chr4.4:306178-306197 None:intergenic 40.0%
ATTGGCATGCATACCTAACA+TGG - chr4.4:306006-306025 None:intergenic 40.0%
ATTTGAGAGCAGGGTTTGAT+TGG - chr4.4:305442-305461 None:intergenic 40.0%
CAAGTGACAATGAACACACA+GGG - chr4.4:305391-305410 None:intergenic 40.0%
CACAGGCAATGGGAAAATAT+GGG + chr4.4:305939-305958 MS.gene040118:intron 40.0%
GGATATTCCAGAGAGAAAGA+TGG + chr4.4:305561-305580 MS.gene040118:CDS 40.0%
TACCAACAAGGGATGTATCT+GGG - chr4.4:306177-306196 None:intergenic 40.0%
TCACAGGCAATGGGAAAATA+TGG + chr4.4:305938-305957 MS.gene040118:intron 40.0%
TCACTGCAAACATGCTTCTA+GGG + chr4.4:306477-306496 MS.gene040118:CDS 40.0%
! ATCCTTTTGACACATTACGC+CGG + chr4.4:306351-306370 MS.gene040118:CDS 40.0%
!! ATATCAAGTGATGGTGTTGC+TGG + chr4.4:305759-305778 MS.gene040118:CDS 40.0%
!! GGGATTCGGTGTTTCAATAT+TGG + chr4.4:305791-305810 MS.gene040118:CDS 40.0%
!! GGTTTCTTAGCTCTATTTCG+AGG + chr4.4:306452-306471 MS.gene040118:CDS 40.0%
AAAGAATCACCTCACCTGTG+TGG - chr4.4:305156-305175 None:intergenic 45.0%
AAGAATCACCTCACCTGTGT+GGG - chr4.4:305155-305174 None:intergenic 45.0%
ACTGGTCACCGTCAGTTTAA+AGG + chr4.4:305711-305730 MS.gene040118:CDS 45.0%
CATGCATTCCGTGAGATTGT+TGG + chr4.4:306422-306441 MS.gene040118:CDS 45.0%
CCTCTTGTCCAACAATCTCA+CGG - chr4.4:306433-306452 None:intergenic 45.0%
CGTGAGATTGTTGGACAAGA+GGG + chr4.4:306431-306450 MS.gene040118:CDS 45.0%
GATGTACCCATCTTTCTCTC+TGG - chr4.4:305571-305590 None:intergenic 45.0%
GGACTTTGCACGTACAAGAT+TGG + chr4.4:305662-305681 MS.gene040118:CDS 45.0%
GGTCAAATCTCTCTCCTCTA+TGG + chr4.4:305312-305331 MS.gene040118:intron 45.0%
GTCACTGCAAACATGCTTCT+AGG + chr4.4:306476-306495 MS.gene040118:CDS 45.0%
TAGAGGAGAGAGATTTGACC+TGG - chr4.4:305312-305331 None:intergenic 45.0%
TTCCCAGATACATCCCTTGT+TGG + chr4.4:306172-306191 MS.gene040118:intron 45.0%
TTGGGAATCACCATGTATCG+AGG + chr4.4:305810-305829 MS.gene040118:CDS 45.0%
TTTAAACTGACGGTGACCAG+TGG - chr4.4:305712-305731 None:intergenic 45.0%
! AAACATGCTTCTAGGGATGG+CGG + chr4.4:306484-306503 MS.gene040118:CDS 45.0%
! GATATTTTCCCACACAGGTG+AGG + chr4.4:305144-305163 MS.gene040118:intron 45.0%
! TGCAAACATGCTTCTAGGGA+TGG + chr4.4:306481-306500 MS.gene040118:CDS 45.0%
! TGGAGCATCACAACTGTATC+TGG + chr4.4:306317-306336 MS.gene040118:CDS 45.0%
!! GGTACATCAAGTGGTTTGCT+GGG + chr4.4:305583-305602 MS.gene040118:CDS 45.0%
!!! AATTGGTGCCACTGCAGTTT+TGG - chr4.4:304970-304989 None:intergenic 45.0%
ATACTTATTCTGGTGCCCGG+AGG - chr4.4:306390-306409 None:intergenic 50.0%
CCGTGAGATTGTTGGACAAG+AGG + chr4.4:306430-306449 MS.gene040118:CDS 50.0%
! AACATGCTTCTAGGGATGGC+GGG + chr4.4:306485-306504 MS.gene040118:CDS 50.0%
! AAGAGGGTCTCCTTGCCTTT+TGG + chr4.4:305096-305115 MS.gene040118:CDS 50.0%
! ATGGCACTGGACTGAATCAG+TGG - chr4.4:305688-305707 None:intergenic 50.0%
! GGGTACATCAAGTGGTTTGC+TGG + chr4.4:305582-305601 MS.gene040118:CDS 50.0%
!! ATGGTGTTGCTGGCTTGTAC+AGG + chr4.4:305769-305788 MS.gene040118:CDS 50.0%
!! TGGTGTTGCTGGCTTGTACA+GGG + chr4.4:305770-305789 MS.gene040118:CDS 50.0%
CGCCGGCGTAATGTGTCAAA+AGG - chr4.4:306356-306375 None:intergenic 55.0%
CGGAGGTTAGCATCATACGC+CGG - chr4.4:306373-306392 None:intergenic 55.0%
CGGCGTATGATGCTAACCTC+CGG + chr4.4:306371-306390 MS.gene040118:CDS 55.0%
GGCGTATGATGCTAACCTCC+GGG + chr4.4:306372-306391 MS.gene040118:CDS 55.0%
GGTGACCTCTCCAAAAGGCA+AGG - chr4.4:305109-305128 None:intergenic 55.0%
TTTGCTGGGAACGTGGCTTC+AGG + chr4.4:305597-305616 MS.gene040118:CDS 55.0%
!! CAAGTGGTTTGCTGGGAACG+TGG + chr4.4:305590-305609 MS.gene040118:CDS 55.0%
!! GCTGGCTTGTACAGGGGATT+CGG + chr4.4:305777-305796 MS.gene040118:CDS 55.0%
!! GGTCTCCTTGCCTTTTGGAG+AGG + chr4.4:305101-305120 MS.gene040118:CDS 55.0%
!! GGTGTTGCTGGCTTGTACAG+GGG + chr4.4:305771-305790 MS.gene040118:CDS 55.0%
!! TGCCTTTTGGAGAGGTCACC+AGG + chr4.4:305109-305128 MS.gene040118:CDS 55.0%
ACTGACGGTGACCAGTGGCA+TGG - chr4.4:305707-305726 None:intergenic 60.0%
GGCCTGGTGACCTCTCCAAA+AGG - chr4.4:305114-305133 None:intergenic 60.0%
TCAGTCCAGTGCCATGCCAC+TGG + chr4.4:305693-305712 MS.gene040118:CDS 60.0%
!! TTCTAGGGATGGCGGGAGCT+GGG + chr4.4:306492-306511 MS.gene040118:CDS 60.0%
CGTGGCTTCAGGCAGTGCTG+CGG + chr4.4:305608-305627 MS.gene040118:CDS 65.0%
GGTGACCAGTGGCATGGCAC+TGG - chr4.4:305701-305720 None:intergenic 65.0%
GTGGCTTCAGGCAGTGCTGC+GGG + chr4.4:305609-305628 MS.gene040118:CDS 65.0%
!! CTTCTAGGGATGGCGGGAGC+TGG + chr4.4:306491-306510 MS.gene040118:CDS 65.0%
!! TCTAGGGATGGCGGGAGCTG+GGG + chr4.4:306493-306512 MS.gene040118:CDS 65.0%
! CGGGAGCTGGGGTGCTTGCT+GGG + chr4.4:306504-306523 MS.gene040118:CDS 70.0%
! GCGGGAGCTGGGGTGCTTGC+TGG + chr4.4:306503-306522 MS.gene040118:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 304906 306598 304906 ID=MS.gene040118
chr4.4 mRNA 304906 306598 304906 ID=MS.gene040118.t1;Parent=MS.gene040118
chr4.4 exon 304906 305160 304906 ID=MS.gene040118.t1.exon1;Parent=MS.gene040118.t1
chr4.4 CDS 304906 305160 304906 ID=cds.MS.gene040118.t1;Parent=MS.gene040118.t1
chr4.4 exon 305519 305890 305519 ID=MS.gene040118.t1.exon2;Parent=MS.gene040118.t1
chr4.4 CDS 305519 305890 305519 ID=cds.MS.gene040118.t1;Parent=MS.gene040118.t1
chr4.4 exon 306287 306598 306287 ID=MS.gene040118.t1.exon3;Parent=MS.gene040118.t1
chr4.4 CDS 306287 306598 306287 ID=cds.MS.gene040118.t1;Parent=MS.gene040118.t1
Gene Sequence

>MS.gene040118

ATGGTAAAATCAGAATCATTTTCAAAGGATTTTGTAATGGGAGGTGCAGCAGCAATCATATCCAAAACTGCAGTGGCACCAATTGAAAGAGTGAAGCTTTTATTACAAAACCAAAGCGAAATGATTAAAAGAGGAAACCTAAAAACACCATACCTTGGTTTGTCTAATACCTTTAAAAGAGTCTTTGCTGAAGAGGGTCTCCTTGCCTTTTGGAGAGGTCACCAGGCCAATGTTATTAGATATTTTCCCACACAGGTGAGGTGATTCTTTGTTATTCATCACATTACTATGAGGTGGTAAATGGATTATAGCCTATTATACTTGTTTTGTAATGTTTTTGGCAAAATGACATGCTTAGTGATATGTTGTGAATGCCCGTGTTGTAATTAGTTCACTAAAAAGTCCAGGTCAAATCTCTCTCCTCTATGGACATTAGTTTAGTTTTTCCCTCAAATAATTCTCAATTTAAATTAAAACATAGTCCCTGTGTGTTCATTGTCACTTGTATAAAAGAATAACCCGTATTTTGTATTCCAATCAAACCCTGCTCTCAAATAACAGTCTTTTTGCATATTAAAAATAATATTTGAGCAAACAGTTAATTGGTCTATAGGCTTTCAATTTTGCATTCAAAGGTTACTTCAAAAGCATCTTTGGATATTCCAGAGAGAAAGATGGGTACATCAAGTGGTTTGCTGGGAACGTGGCTTCAGGCAGTGCTGCGGGAGCTACTACCTCAATACTTTTGTATCATTTGGACTTTGCACGTACAAGATTGGCCACTGATTCAGTCCAGTGCCATGCCACTGGTCACCGTCAGTTTAAAGGACTAATTCATGTATATCGTAAAACAATATCAAGTGATGGTGTTGCTGGCTTGTACAGGGGATTCGGTGTTTCAATATTGGGAATCACCATGTATCGAGGAATGTACTTTGGGATATATGACACCATGAAGCCTATTGTTTTAGTTGGACCTTTTGAGGTAAGCATTTATACATTTATAATTACATTACAACATAGATCTATAATTCACAGGCAATGGGAAAATATGGGGTTAATAATTGCTTCTCTTGGCTCAATTTTTTTCGTCTGTTCCATGTTAGGTATGCATGCCAATATTAGAGTTTTTATATTATGCTGCTTATATTGATGGTCGAACCCTTACCTTTAATTTTAACATACATCTTCAAGAGTAAGACACATGACAAGTTAGACAACAGTAATTTGGAAAAGTAATTTACTGATTTTTCTCATCATAAACTTTTCCCAGATACATCCCTTGTTGGTATAGCATTTTGATGAATTTATGTCAGAGCATTGGTTCTGTTTATTTTTGCCCTTGCATTGGAATATGAGTCTTTTGATTTATATTCTGCAGGGGAACTTCTTTGCTAGTTTCTTGTTAGGTTGGAGCATCACAACTGTATCTGGTGTCTGTGCATATCCTTTTGACACATTACGCCGGCGTATGATGCTAACCTCCGGGCACCAGAATAAGTATTATAATGCAATGCATGCATTCCGTGAGATTGTTGGACAAGAGGGTTTCTTAGCTCTATTTCGAGGAGTCACTGCAAACATGCTTCTAGGGATGGCGGGAGCTGGGGTGCTTGCTGGGTACGATCAGCTAAACCGTATCTCATCTAGACACAGTAACTATAATGAAACGAACCAAAGAGTCATGAAATAA
Protein sequence

>MS.gene040118.t1

MVKSESFSKDFVMGGAAAIISKTAVAPIERVKLLLQNQSEMIKRGNLKTPYLGLSNTFKRVFAEEGLLAFWRGHQANVIRYFPTQAFNFAFKGYFKSIFGYSREKDGYIKWFAGNVASGSAAGATTSILLYHLDFARTRLATDSVQCHATGHRQFKGLIHVYRKTISSDGVAGLYRGFGVSILGITMYRGMYFGIYDTMKPIVLVGPFEGNFFASFLLGWSITTVSGVCAYPFDTLRRRMMLTSGHQNKYYNAMHAFREIVGQEGFLALFRGVTANMLLGMAGAGVLAGYDQLNRISSRHSNYNETNQRVMK