AlfalfaGEDB | MS.gene93756

M. sativa cultivar XinJiangDaYe / MS.gene93756

KEGG: K05863
GO: GO:0003674 GO:0005215 GO:0005575 GO:0005743 GO:0006810 GO:0008150 GO:0016020 GO:0019866 GO:0022857 GO:0031090 GO:0031966 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF00153.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93756.t1	XP_003631113.1	94	302	18	0	11	312	16	317	4.00E-161	577.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93756.t1	Q8LB08	75.5	298	73	0	5	302	6	303	5.9e-129	461.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93756.t1	G7LE57	94.0	302	18	0	11	312	16	317	2.9e-161	577.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene93756	0.81074	9.92E-51	-1.69E-46
MS.gene049609	MS.gene93756	0.803274	3.77E-49	-1.69E-46
MS.gene049633	MS.gene93756	0.805663	1.20E-49	-1.69E-46
MS.gene050772	MS.gene93756	0.802994	4.31E-49	-1.69E-46
MS.gene05080	MS.gene93756	0.807168	5.77E-50	-1.69E-46
MS.gene05105	MS.gene93756	0.801269	9.75E-49	-1.69E-46
MS.gene051666	MS.gene93756	0.807959	3.92E-50	-1.69E-46
MS.gene051734	MS.gene93756	0.801249	9.84E-49	-1.69E-46
MS.gene051838	MS.gene93756	0.835066	2.08E-56	-1.69E-46
MS.gene051858	MS.gene93756	0.804284	2.33E-49	-1.69E-46
MS.gene052164	MS.gene93756	0.809357	1.97E-50	-1.69E-46
MS.gene052414	MS.gene93756	0.81205	5.15E-51	-1.69E-46
MS.gene052481	MS.gene93756	0.80715	5.82E-50	-1.69E-46
MS.gene052484	MS.gene93756	0.823952	1.05E-53	-1.69E-46
MS.gene052966	MS.gene93756	0.814832	1.26E-51	-1.69E-46
MS.gene053449	MS.gene93756	0.818168	2.26E-52	-1.69E-46
MS.gene053824	MS.gene93756	0.812855	3.44E-51	-1.69E-46
MS.gene053912	MS.gene93756	0.813178	2.92E-51	-1.69E-46
MS.gene054600	MS.gene93756	0.806911	6.54E-50	-1.69E-46
MS.gene05494	MS.gene93756	0.828685	7.84E-55	-1.69E-46
MS.gene055577	MS.gene93756	0.820937	5.27E-53	-1.69E-46
MS.gene055722	MS.gene93756	0.803784	2.96E-49	-1.69E-46
MS.gene055741	MS.gene93756	0.801426	9.05E-49	-1.69E-46
MS.gene05589	MS.gene93756	0.808445	3.09E-50	-1.69E-46
MS.gene055941	MS.gene93756	0.800722	1.26E-48	-1.69E-46
MS.gene056387	MS.gene93756	0.809802	1.58E-50	-1.69E-46
MS.gene05664	MS.gene93756	0.814938	1.19E-51	-1.69E-46
MS.gene057076	MS.gene93756	0.81164	6.33E-51	-1.69E-46
MS.gene057734	MS.gene93756	0.81428	1.67E-51	-1.69E-46
MS.gene057857	MS.gene93756	0.805989	1.02E-49	-1.69E-46
MS.gene058838	MS.gene93756	0.831403	1.70E-55	-1.69E-46
MS.gene05913	MS.gene93756	0.809867	1.53E-50	-1.69E-46
MS.gene05934	MS.gene93756	0.810726	9.99E-51	-1.69E-46
MS.gene059718	MS.gene93756	0.804266	2.35E-49	-1.69E-46
MS.gene059902	MS.gene93756	0.80106	1.08E-48	-1.69E-46
MS.gene060762	MS.gene93756	0.813389	2.62E-51	-1.69E-46
MS.gene060837	MS.gene93756	0.816789	4.61E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene62060	MS.gene93756	PPI
MS.gene065947	MS.gene93756	PPI
MS.gene79071	MS.gene93756	PPI
MS.gene064041	MS.gene93756	PPI
MS.gene62064	MS.gene93756	PPI
MS.gene93756	MS.gene010661	PPI
MS.gene93756	MS.gene73296	PPI
MS.gene93756	MS.gene045584	PPI
MS.gene93756	MS.gene042221	PPI
MS.gene93756	MS.gene82276	PPI
MS.gene015010	MS.gene93756	PPI
MS.gene037607	MS.gene93756	PPI
MS.gene93756	MS.gene012626	PPI
MS.gene030289	MS.gene93756	PPI
MS.gene37419	MS.gene93756	PPI
MS.gene93756	MS.gene031696	PPI
MS.gene001110	MS.gene93756	PPI
MS.gene93756	MS.gene99446	PPI
MS.gene93756	MS.gene074632	PPI
MS.gene84655	MS.gene93756	PPI
MS.gene064047	MS.gene93756	PPI
MS.gene93756	MS.gene60471	PPI
MS.gene93756	MS.gene016690	PPI
MS.gene064043	MS.gene93756	PPI
MS.gene074631	MS.gene93756	PPI
MS.gene93756	MS.gene006614	PPI
MS.gene93756	MS.gene62062	PPI
MS.gene75068	MS.gene93756	PPI
MS.gene93756	MS.gene23588	PPI
MS.gene93756	MS.gene46786	PPI
MS.gene065946	MS.gene93756	PPI
MS.gene66338	MS.gene93756	PPI
MS.gene93756	MS.gene012807	PPI
MS.gene93756	MS.gene32809	PPI
MS.gene93756	MS.gene042220	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93756.t1	MTR_8g107270	94.040	302	18	0	11	312	16	317	0.0	595
MS.gene93756.t1	MTR_4g078545	59.233	287	117	0	12	298	74	360	4.44e-124	360
MS.gene93756.t1	MTR_3g028590	58.020	293	123	0	4	296	84	376	3.19e-123	358
MS.gene93756.t1	MTR_3g028590	58.020	293	123	0	4	296	88	380	4.23e-123	358
MS.gene93756.t1	MTR_8g036880	58.446	296	122	1	4	298	93	388	6.49e-120	350
MS.gene93756.t1	MTR_2g098070	58.528	299	120	1	2	296	51	349	3.06e-117	342
MS.gene93756.t1	MTR_3g104500	36.054	294	181	5	8	297	29	319	4.57e-60	195
MS.gene93756.t1	MTR_8g027975	29.392	296	174	9	8	278	46	331	9.73e-32	122
MS.gene93756.t1	MTR_3g104600	32.258	310	161	13	2	280	18	309	1.25e-31	121
MS.gene93756.t1	MTR_5g009500	29.373	303	163	10	8	278	33	316	1.25e-31	121
MS.gene93756.t1	MTR_5g009500	25.854	205	131	5	16	207	144	340	9.65e-12	65.5
MS.gene93756.t1	MTR_6g091800	27.129	317	191	11	9	303	15	313	1.31e-27	109
MS.gene93756.t1	MTR_4g016730	28.571	280	170	8	24	298	100	354	4.38e-27	109
MS.gene93756.t1	MTR_8g096050	27.562	283	185	6	10	288	224	490	8.32e-27	110
MS.gene93756.t1	MTR_7g064980	27.687	307	197	9	12	304	24	319	2.16e-26	106
MS.gene93756.t1	MTR_7g068340	28.520	277	177	9	24	298	98	355	2.60e-26	107
MS.gene93756.t1	MTR_6g091800	26.686	341	186	12	9	303	15	337	6.93e-25	102
MS.gene93756.t1	MTR_6g091800	28.854	253	147	7	9	246	15	249	8.95e-25	101
MS.gene93756.t1	MTR_6g091800	26.909	275	166	9	9	266	15	271	1.87e-24	100
MS.gene93756.t1	MTR_6g091800	27.244	312	167	10	9	278	15	308	2.40e-24	100
MS.gene93756.t1	MTR_3g098460	26.148	283	189	6	10	288	202	468	6.58e-24	101
MS.gene93756.t1	MTR_3g107510	27.240	279	175	9	16	288	125	381	9.97e-24	100
MS.gene93756.t1	MTR_3g107510	27.670	206	120	6	8	206	211	394	2.80e-15	76.3
MS.gene93756.t1	MTR_7g084400	27.046	281	180	7	13	288	213	473	1.50e-23	100
MS.gene93756.t1	MTR_1g012030	26.780	295	187	10	11	298	137	409	1.59e-23	100
MS.gene93756.t1	MTR_1g012030	28.079	203	123	6	8	206	228	411	1.38e-15	77.0
MS.gene93756.t1	MTR_6g091800	28.102	274	146	7	9	246	15	273	7.07e-22	93.6
MS.gene93756.t1	MTR_8g090210	28.387	310	164	10	8	288	105	385	1.49e-21	94.4
MS.gene93756.t1	MTR_6g091800	26.087	299	162	9	9	266	15	295	1.74e-21	92.8
MS.gene93756.t1	MTR_3g074490	27.393	303	172	9	10	288	113	391	8.34e-21	92.4
MS.gene93756.t1	MTR_2g436930	23.148	324	211	8	2	293	1	318	2.98e-20	89.7
MS.gene93756.t1	MTR_4g114320	24.915	293	195	10	11	298	121	393	1.20e-19	89.0
MS.gene93756.t1	MTR_5g081150	25.614	285	196	6	12	288	33	309	4.53e-19	86.7
MS.gene93756.t1	MTR_4g119770	22.393	326	213	7	5	296	3	322	6.29e-18	83.2
MS.gene93756.t1	MTR_6g091800	30.220	182	103	6	9	179	15	183	7.43e-18	80.5
MS.gene93756.t1	MTR_6g092210	25.000	260	176	7	56	303	1	253	1.20e-16	78.6
MS.gene93756.t1	MTR_6g034145	24.535	269	172	8	56	303	1	259	3.40e-16	77.4
MS.gene93756.t1	MTR_7g084400	29.688	192	117	5	13	202	207	382	1.01e-15	77.8
MS.gene93756.t1	MTR_7g109135	23.469	294	209	10	16	302	25	309	1.63e-15	76.3
MS.gene93756.t1	MTR_4g119770	23.462	260	170	5	5	241	3	256	2.94e-15	74.7
MS.gene93756.t1	MTR_6g091800	29.126	206	98	7	9	179	15	207	7.05e-15	72.8
MS.gene93756.t1	MTR_4g005330	21.333	300	192	5	11	288	64	341	2.68e-13	70.1
MS.gene93756.t1	MTR_5g081150	26.667	195	131	3	12	202	33	219	3.73e-13	68.6
MS.gene93756.t1	MTR_5g081150	26.667	195	131	3	12	202	33	219	4.15e-13	68.2
MS.gene93756.t1	MTR_4g057850	23.051	295	196	11	14	295	23	299	6.97e-13	68.6
MS.gene93756.t1	MTR_6g091910	25.373	201	133	4	9	202	15	205	7.54e-13	67.0
MS.gene93756.t1	MTR_4g005330	21.356	295	188	5	16	288	3	275	3.23e-12	66.2
MS.gene93756.t1	MTR_7g109135	22.973	296	201	10	16	302	25	302	4.34e-12	66.2
MS.gene93756.t1	MTR_5g030530	24.211	285	187	8	10	279	5	275	2.25e-11	63.9
MS.gene93756.t1	MTR_1g097890	22.295	305	188	9	14	310	52	315	3.72e-11	63.5
MS.gene93756.t1	MTR_5g032420	21.502	293	198	6	11	274	4	293	5.51e-11	62.8
MS.gene93756.t1	MTR_7g066690	20.803	274	206	6	8	278	12	277	7.30e-11	62.4
MS.gene93756.t1	MTR_3g462140	21.575	292	183	6	8	278	10	276	8.36e-11	62.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene93756.t1	AT5G17400	75.503	298	73	0	5	302	6	303	8.06e-164	458
MS.gene93756.t1	AT4G28390	60.000	290	116	0	9	298	79	368	1.47e-123	359
MS.gene93756.t1	AT4G28390	60.000	290	116	0	9	298	79	368	1.47e-123	359
MS.gene93756.t1	AT5G13490	58.219	292	121	1	8	298	83	374	4.63e-119	348
MS.gene93756.t1	AT5G13490	58.219	292	121	1	8	298	83	374	4.63e-119	348
MS.gene93756.t1	AT3G08580	57.192	292	124	1	8	298	79	370	8.85e-117	342
MS.gene93756.t1	AT3G08580	57.192	292	124	1	8	298	79	370	8.85e-117	342
MS.gene93756.t1	AT5G56450	37.667	300	170	8	9	297	30	323	1.57e-58	191
MS.gene93756.t1	AT2G37890	29.966	297	191	7	11	298	43	331	2.55e-33	125
MS.gene93756.t1	AT4G26180	31.469	286	169	9	9	280	17	289	4.25e-33	124
MS.gene93756.t1	AT4G26180	31.469	286	169	9	9	280	17	289	8.91e-33	124
MS.gene93756.t1	AT4G01100	29.412	306	163	8	7	278	36	322	9.10e-31	119
MS.gene93756.t1	AT5G01500	29.603	277	174	10	24	298	130	387	4.24e-29	115
MS.gene93756.t1	AT1G14560	33.574	277	146	13	24	278	38	298	4.31e-29	113
MS.gene93756.t1	AT3G51870	27.517	298	186	9	24	309	102	381	5.13e-29	114
MS.gene93756.t1	AT1G14560	33.574	277	146	13	24	278	38	298	7.25e-29	113
MS.gene93756.t1	AT4G01100	30.063	316	162	10	7	278	36	336	1.89e-28	113
MS.gene93756.t1	AT4G01100	29.210	291	153	8	22	278	1	272	4.46e-28	110
MS.gene93756.t1	AT3G55640	28.094	299	188	10	3	288	29	313	5.84e-28	111
MS.gene93756.t1	AT3G55640	28.094	299	188	10	3	288	29	313	5.84e-28	111
MS.gene93756.t1	AT3G53940	27.687	307	197	8	11	304	71	365	7.24e-28	111
MS.gene93756.t1	AT3G53940	25.366	205	135	4	8	209	175	364	6.13e-11	63.2
MS.gene93756.t1	AT3G21390	25.714	315	193	7	12	293	18	324	6.94e-24	100
MS.gene93756.t1	AT3G21390	25.879	313	195	6	12	293	40	346	1.14e-23	99.8
MS.gene93756.t1	AT5G61810	28.571	273	167	7	10	278	62	310	1.36e-23	99.4
MS.gene93756.t1	AT5G61810	27.915	283	176	7	10	288	205	463	2.56e-23	100
MS.gene93756.t1	AT3G55640	30.144	209	123	7	3	202	29	223	5.50e-23	95.9
MS.gene93756.t1	AT3G55640	30.144	209	123	7	3	202	29	223	5.50e-23	95.9
MS.gene93756.t1	AT5G07320	26.740	273	168	8	22	288	218	464	6.43e-23	99.0
MS.gene93756.t1	AT5G51050	26.071	280	184	5	13	288	223	483	6.84e-23	99.0
MS.gene93756.t1	AT5G64970	28.146	302	171	10	10	288	132	410	8.46e-21	92.4
MS.gene93756.t1	AT5G64970	28.571	294	164	10	10	280	132	402	1.80e-20	91.3
MS.gene93756.t1	AT5G48970	24.854	342	202	12	2	299	8	338	5.73e-20	89.4
MS.gene93756.t1	AT3G20240	22.330	309	180	7	10	288	49	327	3.02e-19	87.4
MS.gene93756.t1	AT3G53940	28.500	200	123	4	11	202	71	258	3.20e-19	86.3
MS.gene93756.t1	AT5G64970	28.053	303	171	11	10	288	7	286	3.29e-19	86.7
MS.gene93756.t1	AT4G32400	25.347	288	179	10	11	288	112	373	1.20e-18	85.9
MS.gene93756.t1	AT1G78180	28.620	297	164	11	16	288	131	403	1.84e-16	79.7
MS.gene93756.t1	AT1G14560	32.418	182	102	7	117	280	24	202	1.42e-15	75.1
MS.gene93756.t1	AT3G54110	24.643	280	188	9	8	278	10	275	1.01e-14	73.9
MS.gene93756.t1	AT5G48970	24.265	272	163	8	61	299	6	267	4.67e-14	71.2
MS.gene93756.t1	AT5G58970	22.517	302	224	7	8	306	11	305	1.41e-13	70.5
MS.gene93756.t1	AT5G66380	27.368	190	132	4	113	298	5	192	1.06e-12	67.8
MS.gene93756.t1	AT5G01340	26.738	187	124	3	17	199	116	293	4.89e-12	65.9
MS.gene93756.t1	AT1G79900	25.664	226	142	6	58	275	51	258	2.13e-11	63.9
MS.gene93756.t1	AT1G34065	22.295	305	185	9	11	304	80	343	8.74e-11	62.4
MS.gene93756.t1	AT1G34065	22.295	305	185	9	11	304	73	336	9.04e-11	62.4

Find 75 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGCATTATAATACTTATTC+TGG	0.231839	4.2:-252791	None:intergenic
GGGATTCGGTGTTTCAATAT+TGG	0.231853	4.2:+252185	MS.gene93756:CDS
AAGAGGGTCTCCTTGCCTTT+TGG	0.234640	4.2:+251641	MS.gene93756:CDS
GGTTACTTCAAAAGCATCTT+TGG	0.242168	4.2:+251934	MS.gene93756:CDS
AATTGGTGCCACTGCAGTTT+TGG	0.258307	4.2:-251512	None:intergenic
GCGGGAGCTGGAGTGCTTGC+TGG	0.284845	4.2:+252897	MS.gene93756:CDS
ACTGGTCACCGTCAGTTTAA+AGG	0.285006	4.2:+252105	MS.gene93756:CDS
TTCTTTGCTAGTTTCTTGTT+AGG	0.299649	4.2:+252687	MS.gene93756:CDS
GGATTCGGTGTTTCAATATT+GGG	0.326349	4.2:+252186	MS.gene93756:CDS
GTCACTGCAAACATGCTTCT+AGG	0.326966	4.2:+252870	MS.gene93756:CDS
TTGCTAGTTTCTTGTTAGGT+TGG	0.348330	4.2:+252691	MS.gene93756:CDS
AACTAAAACAATAGGCTTCA+TGG	0.352724	4.2:-252250	None:intergenic
GGGTACATCAAGTGGTTTGC+TGG	0.371317	4.2:+251976	MS.gene93756:CDS
AAAAGCTTCACTCTTTCAAT+TGG	0.371863	4.2:-251529	None:intergenic
TCATTTATTTCATGACTCTC+TGG	0.377926	4.2:-252974	None:intergenic
TGGAGCATCACAACTGTATC+TGG	0.393342	4.2:+252711	MS.gene93756:CDS
AAAGGTCCAACTAAAACAAT+AGG	0.409592	4.2:-252258	None:intergenic
GGACTTTGCACGTACAAGAT+TGG	0.415555	4.2:+252056	MS.gene93756:CDS
TTTGCTGGGAATGTGGCTTC+AGG	0.417717	4.2:+251991	MS.gene93756:CDS
ATGGTGTTGCTGGCTTGTAC+AGG	0.427812	4.2:+252163	MS.gene93756:CDS
ATATCAAGTGATGGTGTTGC+TGG	0.431324	4.2:+252153	MS.gene93756:CDS
TGTATCGAGGAATGTACTTT+GGG	0.438762	4.2:+252217	MS.gene93756:CDS
CTTCTAGGGATGGCGGGAGC+TGG	0.440931	4.2:+252885	MS.gene93756:CDS
GATGTACCCATCTTTCTCTC+TGG	0.444325	4.2:-251962	None:intergenic
GTATAAATGCTTACCTCAAA+AGG	0.445098	4.2:-252276	None:intergenic
GCTGGCTTGTACAGGGGATT+CGG	0.454958	4.2:+252171	MS.gene93756:CDS
CATGCATTCCGTGAGATTGT+TGG	0.459314	4.2:+252816	MS.gene93756:CDS
GTGGCTTCAGGCAGTGCTGC+GGG	0.459875	4.2:+252003	MS.gene93756:CDS
CGGGAGCTGGAGTGCTTGCT+GGG	0.485218	4.2:+252898	MS.gene93756:CDS
CGTGAGATTGTTGGACAAGA+GGG	0.488183	4.2:+252825	MS.gene93756:CDS
ATGTATCGAGGAATGTACTT+TGG	0.489826	4.2:+252216	MS.gene93756:CDS
TAAAAGAGTCTTTGCTGAAG+AGG	0.493467	4.2:+251624	MS.gene93756:CDS
ATAATACTTATTCTGGTGCC+CGG	0.509415	4.2:-252784	None:intergenic
TACTGTGTCTAGATGAGATA+CGG	0.509802	4.2:-252935	None:intergenic
AAAAGAGTCTTTGCTGAAGA+GGG	0.517416	4.2:+251625	MS.gene93756:CDS
CGACGGCGTAATGTGTCAAA+AGG	0.519765	4.2:-252747	None:intergenic
GGATATTCCAGAGAGAAAGA+TGG	0.522046	4.2:+251955	MS.gene93756:CDS
GGTGTTGCTGGCTTGTACAG+GGG	0.525521	4.2:+252165	MS.gene93756:CDS
AATATCTAATAACATTGGCC+TGG	0.525661	4.2:-251672	None:intergenic
TGGTGTTGCTGGCTTGTACA+GGG	0.527675	4.2:+252164	MS.gene93756:CDS
GGTGACCAGTGGCATGGCAC+TGG	0.528489	4.2:-252092	None:intergenic
GGTTTCTTAGCTCTATTTCG+AGG	0.528923	4.2:+252846	MS.gene93756:CDS
CAAAGCGAAATGATTAAAAG+AGG	0.529225	4.2:+251562	MS.gene93756:CDS
CGTCGTATGATGCTAACCTC+CGG	0.530080	4.2:+252765	MS.gene93756:CDS
TCACTGCAAACATGCTTCTA+GGG	0.533524	4.2:+252871	MS.gene93756:CDS
GGCCTGGTGACCTCTCCAAA+AGG	0.537477	4.2:-251656	None:intergenic
TCAGTCCAGTGCCATGCCAC+TGG	0.539129	4.2:+252087	MS.gene93756:CDS
GATATTCCAGAGAGAAAGAT+GGG	0.540943	4.2:+251956	MS.gene93756:CDS
CCGTGAGATTGTTGGACAAG+AGG	0.542206	4.2:+252824	MS.gene93756:CDS
GTCGTATGATGCTAACCTCC+GGG	0.550555	4.2:+252766	MS.gene93756:CDS
AACATGCTTCTAGGGATGGC+GGG	0.552983	4.2:+252879	MS.gene93756:CDS
AAAGTACATTCCTCGATACA+TGG	0.553101	4.2:-252214	None:intergenic
TGTGGCTTCAGGCAGTGCTG+CGG	0.558659	4.2:+252002	MS.gene93756:CDS
TAAAGGTATTAGACAAACCA+AGG	0.560990	4.2:-251603	None:intergenic
GGTACATCAAGTGGTTTGCT+GGG	0.566321	4.2:+251977	MS.gene93756:CDS
CAAATGATACAAAAGTATTG+AGG	0.568033	4.2:-252034	None:intergenic
TGCAAACATGCTTCTAGGGA+TGG	0.571454	4.2:+252875	MS.gene93756:CDS
CGTAAAACAATATCAAGTGA+TGG	0.574072	4.2:+252144	MS.gene93756:CDS
AAGAATCACCTCACCTGTGT+GGG	0.577980	4.2:-251697	None:intergenic
GGGAAAATATCTAATAACAT+TGG	0.589711	4.2:-251677	None:intergenic
GTATTAGACAAACCAAGGTA+TGG	0.595810	4.2:-251598	None:intergenic
CAAGTGGTTTGCTGGGAATG+TGG	0.602125	4.2:+251984	MS.gene93756:CDS
AGAAAGATGGGTACATCAAG+TGG	0.605042	4.2:+251968	MS.gene93756:CDS
GAATTAGTCCTTTAAACTGA+CGG	0.606819	4.2:-252113	None:intergenic
CCTCTTGTCCAACAATCTCA+CGG	0.613033	4.2:-252824	None:intergenic
GGTGACCTCTCCAAAAGGCA+AGG	0.614061	4.2:-251651	None:intergenic
AAACATGCTTCTAGGGATGG+CGG	0.631697	4.2:+252878	MS.gene93756:CDS
ACTGACGGTGACCAGTGGCA+TGG	0.636991	4.2:-252098	None:intergenic
AAAGAATCACCTCACCTGTG+TGG	0.643453	4.2:-251698	None:intergenic
TTGGGAATCACCATGTATCG+AGG	0.659477	4.2:+252204	MS.gene93756:CDS
AATCATATCCAAAACTGCAG+TGG	0.672395	4.2:+251504	MS.gene93756:CDS
TTTAAACTGACGGTGACCAG+TGG	0.674236	4.2:-252103	None:intergenic
ATGGCACTGGACTGAATCAG+TGG	0.678456	4.2:-252079	None:intergenic
ATACTTATTCTGGTGCCCGG+AGG	0.698949	4.2:-252781	None:intergenic
CGGAGGTTAGCATCATACGA+CGG	0.749578	4.2:-252764	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATATAAAAACTCTAATAT+TGG	-	chr4.2:252418-252437	None:intergenic	10.0%
!!	AAGATGTATGTTAAAATTAA+AGG	-	chr4.2:252470-252489	None:intergenic	15.0%
!!	TATGTTAAAATTAAAGGTAA+GGG	-	chr4.2:252464-252483	None:intergenic	15.0%
!!	GTATGTTAAAATTAAAGGTA+AGG	-	chr4.2:252465-252484	None:intergenic	20.0%
!!	TTGCATTATAATACTTATTC+TGG	-	chr4.2:252794-252813	None:intergenic	20.0%
!!	TTTGATTGGAATACAAAATA+CGG	-	chr4.2:251822-251841	None:intergenic	20.0%
!!!	ATTTTCAAAGGATTTTGTAA+TGG	+	chr4.2:251468-251487	MS.gene93756:CDS	20.0%
!!!	TTTTCAAAGGATTTTGTAAT+GGG	+	chr4.2:251469-251488	MS.gene93756:CDS	20.0%
!	AACATAGATCTATAATTCAC+AGG	+	chr4.2:252316-252335	MS.gene93756:intron	25.0%
!	GGGAAAATATCTAATAACAT+TGG	-	chr4.2:251680-251699	None:intergenic	25.0%
!	TATTTGAGCAAACAGTTAAT+TGG	+	chr4.2:251883-251902	MS.gene93756:intron	25.0%
!	TTGATTGGAATACAAAATAC+GGG	-	chr4.2:251821-251840	None:intergenic	25.0%
!!	AGAATCAGAATCATTTTCAA+AGG	+	chr4.2:251456-251475	MS.gene93756:CDS	25.0%
!!	CTCAATACTTTTGTATCATT+TGG	+	chr4.2:252035-252054	MS.gene93756:CDS	25.0%
!!	TATTATGCTGCTTATATTGA+TGG	+	chr4.2:252433-252452	MS.gene93756:intron	25.0%
!!!	CAAATGATACAAAAGTATTG+AGG	-	chr4.2:252037-252056	None:intergenic	25.0%
!!!	CTTTTGATTTATATTCTGCA+GGG	+	chr4.2:252660-252679	MS.gene93756:intron	25.0%
!!!	TCTTTTGATTTATATTCTGC+AGG	+	chr4.2:252659-252678	MS.gene93756:intron	25.0%
!!!	TTTTGATTTATATTCTGCAG+GGG	+	chr4.2:252661-252680	MS.gene93756:intron	25.0%
	AAAAGCTTCACTCTTTCAAT+TGG	-	chr4.2:251532-251551	None:intergenic	30.0%
	AAAGGTCCAACTAAAACAAT+AGG	-	chr4.2:252261-252280	None:intergenic	30.0%
	AACTAAAACAATAGGCTTCA+TGG	-	chr4.2:252253-252272	None:intergenic	30.0%
	AATATCTAATAACATTGGCC+TGG	-	chr4.2:251675-251694	None:intergenic	30.0%
	ATCAAAATGCTATACCAACA+AGG	-	chr4.2:252583-252602	None:intergenic	30.0%
	ATCTATAATTCACAGGCAAT+GGG	+	chr4.2:252323-252342	MS.gene93756:intron	30.0%
	ATGAATTTATGTCAGAGCAT+TGG	+	chr4.2:252601-252620	MS.gene93756:intron	30.0%
	CAAAGCGAAATGATTAAAAG+AGG	+	chr4.2:251562-251581	MS.gene93756:CDS	30.0%
	CAAGTTAGACAACAGTAATT+TGG	+	chr4.2:252508-252527	MS.gene93756:intron	30.0%
	CGTAAAACAATATCAAGTGA+TGG	+	chr4.2:252144-252163	MS.gene93756:CDS	30.0%
	GAATTAGTCCTTTAAACTGA+CGG	-	chr4.2:252116-252135	None:intergenic	30.0%
	GTATAAATGCTTACCTCAAA+AGG	-	chr4.2:252279-252298	None:intergenic	30.0%
	TCAAAATGCTATACCAACAA+GGG	-	chr4.2:252582-252601	None:intergenic	30.0%
!	CAAACAGTTAATTGGTCTAT+AGG	+	chr4.2:251891-251910	MS.gene93756:intron	30.0%
!	CTCTTTTAATCATTTCGCTT+TGG	-	chr4.2:251564-251583	None:intergenic	30.0%
!	GCTTTCAATTTTGCATTCAA+AGG	+	chr4.2:251913-251932	MS.gene93756:CDS	30.0%
!	TATTAGATATTTTCCCACAC+AGG	+	chr4.2:251684-251703	MS.gene93756:CDS	30.0%
!	TTCAGCAAAGACTCTTTTAA+AGG	-	chr4.2:251623-251642	None:intergenic	30.0%
!	TTCTTTGCTAGTTTCTTGTT+AGG	+	chr4.2:252687-252706	MS.gene93756:CDS	30.0%
!!	TAAAGGTATTAGACAAACCA+AGG	-	chr4.2:251606-251625	None:intergenic	30.0%
!!!	ATGAAGCCTATTGTTTTAGT+TGG	+	chr4.2:252252-252271	MS.gene93756:CDS	30.0%
	AAAAGAGTCTTTGCTGAAGA+GGG	+	chr4.2:251625-251644	MS.gene93756:CDS	35.0%
	AAAGTACATTCCTCGATACA+TGG	-	chr4.2:252217-252236	None:intergenic	35.0%
	AACCTAAAAACACCATACCT+TGG	+	chr4.2:251586-251605	MS.gene93756:CDS	35.0%
	AAGACTCATATTCCAATGCA+AGG	-	chr4.2:252643-252662	None:intergenic	35.0%
	AATCATATCCAAAACTGCAG+TGG	+	chr4.2:251504-251523	MS.gene93756:CDS	35.0%
	AGACTCATATTCCAATGCAA+GGG	-	chr4.2:252642-252661	None:intergenic	35.0%
	ATAATACTTATTCTGGTGCC+CGG	-	chr4.2:252787-252806	None:intergenic	35.0%
	ATGTATCGAGGAATGTACTT+TGG	+	chr4.2:252216-252235	MS.gene93756:CDS	35.0%
	GATATTCCAGAGAGAAAGAT+GGG	+	chr4.2:251956-251975	MS.gene93756:CDS	35.0%
	GATCTATAATTCACAGGCAA+TGG	+	chr4.2:252322-252341	MS.gene93756:intron	35.0%
	GGGTTAATAATTGCTTCTCT+TGG	+	chr4.2:252354-252373	MS.gene93756:intron	35.0%
	GTATTAGACAAACCAAGGTA+TGG	-	chr4.2:251601-251620	None:intergenic	35.0%
	GTTTATTTATGCCCTTGCAT+TGG	+	chr4.2:252628-252647	MS.gene93756:intron	35.0%
	TAAAAGAGTCTTTGCTGAAG+AGG	+	chr4.2:251624-251643	MS.gene93756:CDS	35.0%
	TACTGTGTCTAGATGAGATA+CGG	-	chr4.2:252938-252957	None:intergenic	35.0%
	TGTATCGAGGAATGTACTTT+GGG	+	chr4.2:252217-252236	MS.gene93756:CDS	35.0%
!	AAAGACTGTTATTTGAGAGC+AGG	-	chr4.2:251846-251865	None:intergenic	35.0%
!	AACCAAGGTATGGTGTTTTT+AGG	-	chr4.2:251591-251610	None:intergenic	35.0%
!	AAGACTGTTATTTGAGAGCA+GGG	-	chr4.2:251845-251864	None:intergenic	35.0%
!	ATGCTGGTCATACATTTTCA+AGG	-	chr4.2:251762-251781	None:intergenic	35.0%
!	TGCTGGTCATACATTTTCAA+GGG	-	chr4.2:251761-251780	None:intergenic	35.0%
!	TTGCTAGTTTCTTGTTAGGT+TGG	+	chr4.2:252691-252710	MS.gene93756:CDS	35.0%
!	TTTTTCGTCTGTTCCATGTT+AGG	+	chr4.2:252384-252403	MS.gene93756:intron	35.0%
!!	GGATTCGGTGTTTCAATATT+GGG	+	chr4.2:252186-252205	MS.gene93756:CDS	35.0%
!!	GGTTACTTCAAAAGCATCTT+TGG	+	chr4.2:251934-251953	MS.gene93756:CDS	35.0%
!!	TCAAAGGATTTTGTAATGGG+AGG	+	chr4.2:251472-251491	MS.gene93756:CDS	35.0%
!!!	TGTTTTAGTTGGACCTTTTG+AGG	+	chr4.2:252263-252282	MS.gene93756:CDS	35.0%
	ACAGGCAATGGGAAAATATG+GGG	+	chr4.2:252334-252353	MS.gene93756:intron	40.0%
	AGAAAGATGGGTACATCAAG+TGG	+	chr4.2:251968-251987	MS.gene93756:CDS	40.0%
	ATACCAACAAGGGATGTATC+TGG	-	chr4.2:252572-252591	None:intergenic	40.0%
	ATTGGCATGCATACCTAACA+TGG	-	chr4.2:252400-252419	None:intergenic	40.0%
	ATTTGAGAGCAGGGTTTGAT+TGG	-	chr4.2:251836-251855	None:intergenic	40.0%
	CACAGGCAATGGGAAAATAT+GGG	+	chr4.2:252333-252352	MS.gene93756:intron	40.0%
	GGATATTCCAGAGAGAAAGA+TGG	+	chr4.2:251955-251974	MS.gene93756:CDS	40.0%
	TACCAACAAGGGATGTATCT+GGG	-	chr4.2:252571-252590	None:intergenic	40.0%
	TCACAGGCAATGGGAAAATA+TGG	+	chr4.2:252332-252351	MS.gene93756:intron	40.0%
	TCACTGCAAACATGCTTCTA+GGG	+	chr4.2:252871-252890	MS.gene93756:CDS	40.0%
!!	ATATCAAGTGATGGTGTTGC+TGG	+	chr4.2:252153-252172	MS.gene93756:CDS	40.0%
!!	GCTGGTCATACATTTTCAAG+GGG	-	chr4.2:251760-251779	None:intergenic	40.0%
!!	GGGATTCGGTGTTTCAATAT+TGG	+	chr4.2:252185-252204	MS.gene93756:CDS	40.0%
!!	GGTTTCTTAGCTCTATTTCG+AGG	+	chr4.2:252846-252865	MS.gene93756:CDS	40.0%
	AAAGAATCACCTCACCTGTG+TGG	-	chr4.2:251701-251720	None:intergenic	45.0%
	AAGAATCACCTCACCTGTGT+GGG	-	chr4.2:251700-251719	None:intergenic	45.0%
	ACTGGTCACCGTCAGTTTAA+AGG	+	chr4.2:252105-252124	MS.gene93756:CDS	45.0%
	CAATGAACACATAGCGATGC+TGG	-	chr4.2:251778-251797	None:intergenic	45.0%
	CATGCATTCCGTGAGATTGT+TGG	+	chr4.2:252816-252835	MS.gene93756:CDS	45.0%
	CCTCTTGTCCAACAATCTCA+CGG	-	chr4.2:252827-252846	None:intergenic	45.0%
	CGTGAGATTGTTGGACAAGA+GGG	+	chr4.2:252825-252844	MS.gene93756:CDS	45.0%
	GATGTACCCATCTTTCTCTC+TGG	-	chr4.2:251965-251984	None:intergenic	45.0%
	GGACTTTGCACGTACAAGAT+TGG	+	chr4.2:252056-252075	MS.gene93756:CDS	45.0%
	GTCACTGCAAACATGCTTCT+AGG	+	chr4.2:252870-252889	MS.gene93756:CDS	45.0%
	TTCCCAGATACATCCCTTGT+TGG	+	chr4.2:252566-252585	MS.gene93756:intron	45.0%
	TTGGGAATCACCATGTATCG+AGG	+	chr4.2:252204-252223	MS.gene93756:CDS	45.0%
	TTTAAACTGACGGTGACCAG+TGG	-	chr4.2:252106-252125	None:intergenic	45.0%
!	AAACATGCTTCTAGGGATGG+CGG	+	chr4.2:252878-252897	MS.gene93756:CDS	45.0%
!	GATATTTTCCCACACAGGTG+AGG	+	chr4.2:251689-251708	MS.gene93756:intron	45.0%
!	TGCAAACATGCTTCTAGGGA+TGG	+	chr4.2:252875-252894	MS.gene93756:CDS	45.0%
!	TGGAGCATCACAACTGTATC+TGG	+	chr4.2:252711-252730	MS.gene93756:CDS	45.0%
!!	GGTACATCAAGTGGTTTGCT+GGG	+	chr4.2:251977-251996	MS.gene93756:CDS	45.0%
!!!	AATTGGTGCCACTGCAGTTT+TGG	-	chr4.2:251515-251534	None:intergenic	45.0%
	ATACTTATTCTGGTGCCCGG+AGG	-	chr4.2:252784-252803	None:intergenic	50.0%
	CCGTGAGATTGTTGGACAAG+AGG	+	chr4.2:252824-252843	MS.gene93756:CDS	50.0%
	CGACGGCGTAATGTGTCAAA+AGG	-	chr4.2:252750-252769	None:intergenic	50.0%
	CGGAGGTTAGCATCATACGA+CGG	-	chr4.2:252767-252786	None:intergenic	50.0%
	CGTCGTATGATGCTAACCTC+CGG	+	chr4.2:252765-252784	MS.gene93756:CDS	50.0%
	GTCGTATGATGCTAACCTCC+GGG	+	chr4.2:252766-252785	MS.gene93756:CDS	50.0%
	TTTGCTGGGAATGTGGCTTC+AGG	+	chr4.2:251991-252010	MS.gene93756:CDS	50.0%
!	AACATGCTTCTAGGGATGGC+GGG	+	chr4.2:252879-252898	MS.gene93756:CDS	50.0%
!	AAGAGGGTCTCCTTGCCTTT+TGG	+	chr4.2:251641-251660	MS.gene93756:CDS	50.0%
!	ATGGCACTGGACTGAATCAG+TGG	-	chr4.2:252082-252101	None:intergenic	50.0%
!	GGGTACATCAAGTGGTTTGC+TGG	+	chr4.2:251976-251995	MS.gene93756:CDS	50.0%
!!	ATGGTGTTGCTGGCTTGTAC+AGG	+	chr4.2:252163-252182	MS.gene93756:CDS	50.0%
!!	CAAGTGGTTTGCTGGGAATG+TGG	+	chr4.2:251984-252003	MS.gene93756:CDS	50.0%
!!	TGGTGTTGCTGGCTTGTACA+GGG	+	chr4.2:252164-252183	MS.gene93756:CDS	50.0%
	GGTGACCTCTCCAAAAGGCA+AGG	-	chr4.2:251654-251673	None:intergenic	55.0%
!!	GCTGGCTTGTACAGGGGATT+CGG	+	chr4.2:252171-252190	MS.gene93756:CDS	55.0%
!!	GGTCTCCTTGCCTTTTGGAG+AGG	+	chr4.2:251646-251665	MS.gene93756:CDS	55.0%
!!	GGTGTTGCTGGCTTGTACAG+GGG	+	chr4.2:252165-252184	MS.gene93756:CDS	55.0%
!!	TGCCTTTTGGAGAGGTCACC+AGG	+	chr4.2:251654-251673	MS.gene93756:CDS	55.0%
	ACTGACGGTGACCAGTGGCA+TGG	-	chr4.2:252101-252120	None:intergenic	60.0%
	GGCCTGGTGACCTCTCCAAA+AGG	-	chr4.2:251659-251678	None:intergenic	60.0%
	TCAGTCCAGTGCCATGCCAC+TGG	+	chr4.2:252087-252106	MS.gene93756:CDS	60.0%
	TGTGGCTTCAGGCAGTGCTG+CGG	+	chr4.2:252002-252021	MS.gene93756:CDS	60.0%
	GGTGACCAGTGGCATGGCAC+TGG	-	chr4.2:252095-252114	None:intergenic	65.0%
	GTGGCTTCAGGCAGTGCTGC+GGG	+	chr4.2:252003-252022	MS.gene93756:CDS	65.0%
!	CGGGAGCTGGAGTGCTTGCT+GGG	+	chr4.2:252898-252917	MS.gene93756:CDS	65.0%
!!	CTTCTAGGGATGGCGGGAGC+TGG	+	chr4.2:252885-252904	MS.gene93756:CDS	65.0%
!	GCGGGAGCTGGAGTGCTTGC+TGG	+	chr4.2:252897-252916	MS.gene93756:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	251445	252992	251445	ID=MS.gene93756
chr4.2	mRNA	251445	252992	251445	ID=MS.gene93756.t1;Parent=MS.gene93756
chr4.2	exon	251445	251705	251445	ID=MS.gene93756.t1.exon1;Parent=MS.gene93756.t1
chr4.2	CDS	251445	251705	251445	ID=cds.MS.gene93756.t1;Parent=MS.gene93756.t1
chr4.2	exon	251913	252284	251913	ID=MS.gene93756.t1.exon2;Parent=MS.gene93756.t1
chr4.2	CDS	251913	252284	251913	ID=cds.MS.gene93756.t1;Parent=MS.gene93756.t1
chr4.2	exon	252681	252992	252681	ID=MS.gene93756.t1.exon3;Parent=MS.gene93756.t1
chr4.2	CDS	252681	252992	252681	ID=cds.MS.gene93756.t1;Parent=MS.gene93756.t1

Gene Sequence

>MS.gene93756

ATGGTAAAATCAGAATCAGAATCATTTTCAAAGGATTTTGTAATGGGAGGTGCAGCAGCAATCATATCCAAAACTGCAGTGGCACCAATTGAAAGAGTGAAGCTTTTATTACAAAACCAAAGCGAAATGATTAAAAGAGGAAACCTAAAAACACCATACCTTGGTTTGTCTAATACCTTTAAAAGAGTCTTTGCTGAAGAGGGTCTCCTTGCCTTTTGGAGAGGTCACCAGGCCAATGTTATTAGATATTTTCCCACACAGGTGAGGTGATTCTTTGTTATTCATCACATTACTACTATAATATATTATTTACCCCTTGAAAATGTATGACCAGCATCGCTATGTGTTCATTGTCACTTGTATAAAAGAATAACCCGTATTTTGTATTCCAATCAAACCCTGCTCTCAAATAACAGTCTTTTTGCATATTAAAAATAATATTTGAGCAAACAGTTAATTGGTCTATAGGCTTTCAATTTTGCATTCAAAGGTTACTTCAAAAGCATCTTTGGATATTCCAGAGAGAAAGATGGGTACATCAAGTGGTTTGCTGGGAATGTGGCTTCAGGCAGTGCTGCGGGAGCTACTACCTCAATACTTTTGTATCATTTGGACTTTGCACGTACAAGATTGGCCACTGATTCAGTCCAGTGCCATGCCACTGGTCACCGTCAGTTTAAAGGACTAATTCATGTATATCGTAAAACAATATCAAGTGATGGTGTTGCTGGCTTGTACAGGGGATTCGGTGTTTCAATATTGGGAATCACCATGTATCGAGGAATGTACTTTGGGATATATGACACCATGAAGCCTATTGTTTTAGTTGGACCTTTTGAGGTAAGCATTTATACATTTATAATTACATTACAACATAGATCTATAATTCACAGGCAATGGGAAAATATGGGGTTAATAATTGCTTCTCTTGGCTCAATTTTTTTCGTCTGTTCCATGTTAGGTATGCATGCCAATATTAGAGTTTTTATATTATGCTGCTTATATTGATGGTCGAACCCTTACCTTTAATTTTAACATACATCTTCAAGAGTATGACACATGACAAGTTAGACAACAGTAATTTGGAAAAGTAATTTACTGATTTTTCTCATCATAAACTTTTCCCAGATACATCCCTTGTTGGTATAGCATTTTGATGAATTTATGTCAGAGCATTGGTTCTGTTTATTTATGCCCTTGCATTGGAATATGAGTCTTTTGATTTATATTCTGCAGGGGAACTTCTTTGCTAGTTTCTTGTTAGGTTGGAGCATCACAACTGTATCTGGTGTCTGTGCATATCCTTTTGACACATTACGCCGTCGTATGATGCTAACCTCCGGGCACCAGAATAAGTATTATAATGCAATGCATGCATTCCGTGAGATTGTTGGACAAGAGGGTTTCTTAGCTCTATTTCGAGGAGTCACTGCAAACATGCTTCTAGGGATGGCGGGAGCTGGAGTGCTTGCTGGGTACGATCAGCTAAACCGTATCTCATCTAGACACAGTAACTATAATGAAACGAACCAGAGAGTCATGAAATAA

Protein sequence

>MS.gene93756.t1

MVKSESESFSKDFVMGGAAAIISKTAVAPIERVKLLLQNQSEMIKRGNLKTPYLGLSNTFKRVFAEEGLLAFWRGHQANVIRYFPTQAFNFAFKGYFKSIFGYSREKDGYIKWFAGNVASGSAAGATTSILLYHLDFARTRLATDSVQCHATGHRQFKGLIHVYRKTISSDGVAGLYRGFGVSILGITMYRGMYFGIYDTMKPIVLVGPFEGNFFASFLLGWSITTVSGVCAYPFDTLRRRMMLTSGHQNKYYNAMHAFREIVGQEGFLALFRGVTANMLLGMAGAGVLAGYDQLNRISSRHSNYNETNQRVMK