Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63789.t1 | XP_003617604.1 | 79.7 | 192 | 6 | 2 | 1 | 159 | 3 | 194 | 9.40E-66 | 259.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63789.t1 | G7K8L0 | 79.7 | 192 | 6 | 2 | 1 | 159 | 3 | 194 | 6.8e-66 | 259.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene63789 | -0.802128 | 6.50E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene63789 | -0.824325 | 8.58E-54 | -1.69E-46 |
| MS.gene050577 | MS.gene63789 | -0.809693 | 1.67E-50 | -1.69E-46 |
| MS.gene050904 | MS.gene63789 | -0.810424 | 1.16E-50 | -1.69E-46 |
| MS.gene052606 | MS.gene63789 | 0.809462 | 1.87E-50 | -1.69E-46 |
| MS.gene05470 | MS.gene63789 | -0.810419 | 1.16E-50 | -1.69E-46 |
| MS.gene055502 | MS.gene63789 | -0.801699 | 7.96E-49 | -1.69E-46 |
| MS.gene055748 | MS.gene63789 | -0.817271 | 3.60E-52 | -1.69E-46 |
| MS.gene055750 | MS.gene63789 | -0.807766 | 4.30E-50 | -1.69E-46 |
| MS.gene055751 | MS.gene63789 | -0.824272 | 8.83E-54 | -1.69E-46 |
| MS.gene05589 | MS.gene63789 | -0.830952 | 2.20E-55 | -1.69E-46 |
| MS.gene056692 | MS.gene63789 | -0.80611 | 9.65E-50 | -1.69E-46 |
| MS.gene058334 | MS.gene63789 | 0.827208 | 1.78E-54 | -1.69E-46 |
| MS.gene058838 | MS.gene63789 | -0.807538 | 4.81E-50 | -1.69E-46 |
| MS.gene059023 | MS.gene63789 | -0.801065 | 1.07E-48 | -1.69E-46 |
| MS.gene060762 | MS.gene63789 | -0.82299 | 1.76E-53 | -1.69E-46 |
| MS.gene060982 | MS.gene63789 | -0.81364 | 2.31E-51 | -1.69E-46 |
| MS.gene061044 | MS.gene63789 | 0.819938 | 8.93E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63789.t1 | MTR_5g093430 | 79.688 | 192 | 6 | 2 | 1 | 159 | 3 | 194 | 2.91e-85 | 248 |
| MS.gene63789.t1 | MTR_5g093440 | 67.380 | 187 | 27 | 3 | 1 | 154 | 3 | 188 | 5.88e-69 | 207 |
| MS.gene63789.t1 | MTR_3g053160 | 41.243 | 177 | 72 | 4 | 1 | 154 | 1 | 168 | 1.27e-22 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTGTTACCTTCCTCTCTT+AGG | 0.249740 | 5.3:+75169512 | MS.gene63789:CDS |
| TGTTCTAGCATTTCATGTTT+CGG | 0.336351 | 5.3:-75169655 | None:intergenic |
| AGATTTATTAACTTTGTTCA+TGG | 0.336906 | 5.3:-75169443 | None:intergenic |
| GCAAAAGCTGAAGCTTTCAT+TGG | 0.375645 | 5.3:+75169943 | MS.gene63789:CDS |
| GAATGTTGAAGATTAAATAA+AGG | 0.422149 | 5.3:-75169543 | None:intergenic |
| AGATGCAGAGAGAGGGCTGC+TGG | 0.439749 | 5.3:+75169986 | MS.gene63789:CDS |
| CCTAAGAGAGGAAGGTAACA+AGG | 0.502209 | 5.3:-75169512 | None:intergenic |
| AACATTCTTATCATTGCACT+TGG | 0.519113 | 5.3:+75169559 | MS.gene63789:CDS |
| TGGTGCTAGTAAAGTGGATA+AGG | 0.531732 | 5.3:+75169795 | MS.gene63789:CDS |
| AAGATTAAATAAAGGTAACT+AGG | 0.532639 | 5.3:-75169535 | None:intergenic |
| GATGCAGAGAGAGGGCTGCT+GGG | 0.533020 | 5.3:+75169987 | MS.gene63789:CDS |
| ATTGCACTTGGAGCTGAAGC+CGG | 0.547486 | 5.3:+75169571 | MS.gene63789:CDS |
| TAACTAGGCCTAAGAGAGGA+AGG | 0.551563 | 5.3:-75169520 | None:intergenic |
| ACAAAATGAAAAGAAGCCTA+AGG | 0.566232 | 5.3:+75169732 | MS.gene63789:CDS |
| CAGTTGAAGATGCAGAGAGA+GGG | 0.576252 | 5.3:+75169979 | MS.gene63789:CDS |
| GTAACAAGGGTGTGATCACA+AGG | 0.579955 | 5.3:-75169498 | None:intergenic |
| GTATGTTGGTGCTAGTAAAG+TGG | 0.582981 | 5.3:+75169789 | MS.gene63789:CDS |
| AGAGAACACCGACAAAAGAC+CGG | 0.585897 | 5.3:-75169590 | None:intergenic |
| GCAGTTGAAGATGCAGAGAG+AGG | 0.589013 | 5.3:+75169978 | MS.gene63789:CDS |
| AAGGTAACTAGGCCTAAGAG+AGG | 0.633233 | 5.3:-75169524 | None:intergenic |
| CTAAGAGAGGAAGGTAACAA+GGG | 0.639907 | 5.3:-75169511 | None:intergenic |
| GAGAAGAAGATTGTGTATGT+TGG | 0.645099 | 5.3:+75169775 | MS.gene63789:CDS |
| AAATGAAAAGAAGCCTAAGG+TGG | 0.649751 | 5.3:+75169735 | MS.gene63789:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTCTATATAAAAAATACT+TGG | - | chr5.3:75169841-75169860 | None:intergenic | 15.0% |
| !! | AAGATTAAATAAAGGTAACT+AGG | - | chr5.3:75169538-75169557 | None:intergenic | 20.0% |
| !! | GAATGTTGAAGATTAAATAA+AGG | - | chr5.3:75169546-75169565 | None:intergenic | 20.0% |
| !! | TAAAAAATACTTGGCATTGA+AGG | - | chr5.3:75169832-75169851 | None:intergenic | 25.0% |
| AACATTCTTATCATTGCACT+TGG | + | chr5.3:75169559-75169578 | MS.gene63789:CDS | 30.0% | |
| ACAAAATGAAAAGAAGCCTA+AGG | + | chr5.3:75169732-75169751 | MS.gene63789:CDS | 30.0% | |
| AGAAGATGATTTAGAAGTGA+AGG | + | chr5.3:75169864-75169883 | MS.gene63789:intron | 30.0% | |
| GAAGTAGAAGATGAGATTTA+TGG | + | chr5.3:75169898-75169917 | MS.gene63789:intron | 30.0% | |
| TGTTCTAGCATTTCATGTTT+CGG | - | chr5.3:75169658-75169677 | None:intergenic | 30.0% | |
| ! | GATGAGATTTATGGTGTTAA+TGG | + | chr5.3:75169907-75169926 | MS.gene63789:intron | 30.0% |
| AAATGAAAAGAAGCCTAAGG+TGG | + | chr5.3:75169735-75169754 | MS.gene63789:CDS | 35.0% | |
| GAGAAGAAGATTGTGTATGT+TGG | + | chr5.3:75169775-75169794 | MS.gene63789:CDS | 35.0% | |
| TGATTTAGAAGTGAAGGATG+AGG | + | chr5.3:75169870-75169889 | MS.gene63789:intron | 35.0% | |
| !! | ACAGATTTTTCAACCACCTT+AGG | - | chr5.3:75169751-75169770 | None:intergenic | 35.0% |
| !!! | TGTTGCTTTTTGTCTTCAGA+AGG | - | chr5.3:75169619-75169638 | None:intergenic | 35.0% |
| CTAAGAGAGGAAGGTAACAA+GGG | - | chr5.3:75169514-75169533 | None:intergenic | 40.0% | |
| GCAAAAGCTGAAGCTTTCAT+TGG | + | chr5.3:75169943-75169962 | MS.gene63789:CDS | 40.0% | |
| ! | TGGTGCTAGTAAAGTGGATA+AGG | + | chr5.3:75169795-75169814 | MS.gene63789:CDS | 40.0% |
| !! | GTATGTTGGTGCTAGTAAAG+TGG | + | chr5.3:75169789-75169808 | MS.gene63789:CDS | 40.0% |
| AAGGTAACTAGGCCTAAGAG+AGG | - | chr5.3:75169527-75169546 | None:intergenic | 45.0% | |
| AGAGAACACCGACAAAAGAC+CGG | - | chr5.3:75169593-75169612 | None:intergenic | 45.0% | |
| CAGTTGAAGATGCAGAGAGA+GGG | + | chr5.3:75169979-75169998 | MS.gene63789:CDS | 45.0% | |
| CCTAAGAGAGGAAGGTAACA+AGG | - | chr5.3:75169515-75169534 | None:intergenic | 45.0% | |
| CCTTGTTACCTTCCTCTCTT+AGG | + | chr5.3:75169512-75169531 | MS.gene63789:CDS | 45.0% | |
| TAACTAGGCCTAAGAGAGGA+AGG | - | chr5.3:75169523-75169542 | None:intergenic | 45.0% | |
| ! | GTAACAAGGGTGTGATCACA+AGG | - | chr5.3:75169501-75169520 | None:intergenic | 45.0% |
| ATTGCACTTGGAGCTGAAGC+CGG | + | chr5.3:75169571-75169590 | MS.gene63789:CDS | 50.0% | |
| GCAGTTGAAGATGCAGAGAG+AGG | + | chr5.3:75169978-75169997 | MS.gene63789:CDS | 50.0% | |
| ! | AGCTGAAGCCGGTCTTTTGT+CGG | + | chr5.3:75169582-75169601 | MS.gene63789:CDS | 50.0% |
| AGATGCAGAGAGAGGGCTGC+TGG | + | chr5.3:75169986-75170005 | MS.gene63789:CDS | 60.0% | |
| GATGCAGAGAGAGGGCTGCT+GGG | + | chr5.3:75169987-75170006 | MS.gene63789:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 75169445 | 75170017 | 75169445 | ID=MS.gene63789 |
| chr5.3 | mRNA | 75169445 | 75170017 | 75169445 | ID=MS.gene63789.t1;Parent=MS.gene63789 |
| chr5.3 | exon | 75169445 | 75169841 | 75169445 | ID=MS.gene63789.t1.exon1;Parent=MS.gene63789.t1 |
| chr5.3 | CDS | 75169445 | 75169841 | 75169445 | ID=cds.MS.gene63789.t1;Parent=MS.gene63789.t1 |
| chr5.3 | exon | 75169935 | 75170017 | 75169935 | ID=MS.gene63789.t1.exon2;Parent=MS.gene63789.t1 |
| chr5.3 | CDS | 75169935 | 75170017 | 75169935 | ID=cds.MS.gene63789.t1;Parent=MS.gene63789.t1 |
| Gene Sequence |
| Protein sequence |
