AlfalfaGEDB | MS.gene64425

M. sativa cultivar XinJiangDaYe / MS.gene64425

KEGG: -
GO: GO:0003674 GO:0005488 GO:0030145 GO:0043167 GO:0043169 GO:0045735 GO:0046872 GO:0046914
PFAM: PF00190.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64425.t1	XP_013463539.1	94.7	208	11	0	1	208	3	210	3.00E-104	387.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64425.t1	Q9ZRA4	74.5	208	52	1	1	207	1	208	4.7e-82	305.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64425.t1	A0A072VHE3	94.2	208	12	0	1	208	3	210	2.2e-104	387.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050020	MS.gene64425	0.857675	1.39E-62	-1.69E-46
MS.gene050438	MS.gene64425	0.813999	1.93E-51	-1.69E-46
MS.gene050584	MS.gene64425	0.826082	3.30E-54	-1.69E-46
MS.gene050585	MS.gene64425	0.80691	6.54E-50	-1.69E-46
MS.gene050586	MS.gene64425	0.84005	1.10E-57	-1.69E-46
MS.gene050807	MS.gene64425	0.833209	6.08E-56	-1.69E-46
MS.gene05143	MS.gene64425	0.807017	6.21E-50	-1.69E-46
MS.gene051626	MS.gene64425	0.839458	1.57E-57	-1.69E-46
MS.gene051789	MS.gene64425	0.866317	3.11E-65	-1.69E-46
MS.gene052142	MS.gene64425	0.832377	9.79E-56	-1.69E-46
MS.gene052187	MS.gene64425	0.811445	6.98E-51	-1.69E-46
MS.gene052665	MS.gene64425	0.817425	3.32E-52	-1.69E-46
MS.gene052698	MS.gene64425	0.824115	9.61E-54	-1.69E-46
MS.gene053074	MS.gene64425	0.883884	3.07E-71	-1.69E-46
MS.gene053184	MS.gene64425	0.811709	6.11E-51	-1.69E-46
MS.gene053383	MS.gene64425	0.804129	2.51E-49	-1.69E-46
MS.gene053630	MS.gene64425	0.816581	5.14E-52	-1.69E-46
MS.gene05456	MS.gene64425	0.849016	4.24E-60	-1.69E-46
MS.gene054596	MS.gene64425	0.826578	2.51E-54	-1.69E-46
MS.gene055359	MS.gene64425	0.856156	3.89E-62	-1.69E-46
MS.gene055860	MS.gene64425	0.800921	1.15E-48	-1.69E-46
MS.gene056069	MS.gene64425	-0.800914	1.15E-48	-1.69E-46
MS.gene058168	MS.gene64425	0.902765	6.93E-79	-1.69E-46
MS.gene059006	MS.gene64425	0.888146	7.65E-73	-1.69E-46
MS.gene059124	MS.gene64425	0.856365	3.38E-62	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64425.t1	MTR_2g041570	94.712	208	11	0	1	208	3	210	9.00e-143	397
MS.gene64425.t1	MTR_2g041580	94.231	208	12	0	1	208	3	210	1.44e-142	396
MS.gene64425.t1	MTR_2g041550	85.577	208	30	0	1	208	3	210	2.55e-127	357
MS.gene64425.t1	MTR_8g020590	64.904	208	73	0	1	208	1	208	1.01e-92	270
MS.gene64425.t1	MTR_8g020630	55.981	209	88	3	2	208	3	209	2.17e-79	236
MS.gene64425.t1	MTR_8g020650	56.995	193	81	2	16	208	19	209	3.58e-77	231
MS.gene64425.t1	MTR_8g020670	55.024	209	90	3	2	208	3	209	1.33e-76	229
MS.gene64425.t1	MTR_8g020610	51.923	208	94	2	5	208	2	207	1.03e-71	216
MS.gene64425.t1	MTR_2g044040	49.761	209	101	3	2	208	3	209	8.40e-70	212
MS.gene64425.t1	MTR_2g045100	51.196	209	98	3	2	208	3	209	2.67e-69	211
MS.gene64425.t1	MTR_3g019710	49.761	209	101	3	2	208	3	209	6.75e-67	204
MS.gene64425.t1	MTR_2g036120	50.481	208	99	3	2	207	3	208	1.84e-66	203
MS.gene64425.t1	MTR_3g019650	49.519	208	102	3	2	208	3	208	8.30e-66	202
MS.gene64425.t1	MTR_3g019680	49.519	208	102	3	2	208	3	208	3.21e-65	200
MS.gene64425.t1	MTR_0284s0010	47.847	209	105	3	2	208	3	209	1.72e-61	191
MS.gene64425.t1	MTR_8g020770	45.498	211	111	3	1	208	3	212	1.32e-60	189
MS.gene64425.t1	MTR_4g010340	47.596	208	106	3	1	208	1	205	1.39e-60	188
MS.gene64425.t1	MTR_5g031460	45.813	203	108	2	6	208	9	209	1.99e-59	186
MS.gene64425.t1	MTR_8g020750	45.498	211	111	3	1	208	3	212	3.17e-59	185
MS.gene64425.t1	MTR_7g011340	47.938	194	99	2	15	208	18	209	1.15e-58	184
MS.gene64425.t1	MTR_8g020760	45.283	212	109	4	1	208	3	211	1.35e-56	178
MS.gene64425.t1	MTR_3g073170	43.915	189	100	4	21	208	27	210	2.02e-56	178
MS.gene64425.t1	MTR_2g019780	41.981	212	112	5	3	208	6	212	6.21e-53	169
MS.gene64425.t1	MTR_3g019810	45.854	205	101	4	2	204	109	305	2.47e-51	168
MS.gene64425.t1	MTR_8g020763	42.778	180	100	3	30	208	13	190	6.31e-49	158
MS.gene64425.t1	MTR_2g031270	41.489	188	103	3	18	201	26	210	1.81e-42	142
MS.gene64425.t1	MTR_2g030855	40.957	188	104	3	18	201	25	209	2.26e-42	142
MS.gene64425.t1	MTR_1g079490	40.758	211	109	5	6	201	5	214	6.63e-41	139
MS.gene64425.t1	MTR_4g017050	40.094	212	110	5	5	201	4	213	1.26e-40	138
MS.gene64425.t1	MTR_5g046410	40.094	212	110	5	5	201	4	213	1.48e-40	137
MS.gene64425.t1	MTR_5g046430	40.094	212	110	5	5	201	4	213	1.48e-40	137
MS.gene64425.t1	MTR_4g017030	40.094	212	110	5	5	201	4	213	1.48e-40	137
MS.gene64425.t1	MTR_1g112200	37.438	203	118	3	1	201	9	204	9.28e-40	135
MS.gene64425.t1	MTR_6g005310	39.252	214	115	5	1	201	1	212	2.31e-39	134
MS.gene64425.t1	MTR_1g085140	40.107	187	110	1	21	205	30	216	2.34e-39	134
MS.gene64425.t1	MTR_6g005360	39.252	214	115	5	1	201	1	212	3.19e-39	134
MS.gene64425.t1	MTR_7g111240	41.176	187	105	2	21	205	26	209	6.51e-39	133
MS.gene64425.t1	MTR_2g019250	38.679	212	113	5	5	201	4	213	6.81e-39	133
MS.gene64425.t1	MTR_4g017040	42.021	188	100	4	21	201	28	213	1.11e-38	133
MS.gene64425.t1	MTR_6g005340	38.785	214	116	5	1	201	1	212	1.60e-38	132
MS.gene64425.t1	MTR_7g111240	40.642	187	109	1	21	205	26	212	2.06e-38	132
MS.gene64425.t1	MTR_1g060120	41.317	167	97	1	38	204	56	221	2.15e-38	132
MS.gene64425.t1	MTR_1g023770	40.476	168	96	2	38	201	39	206	4.49e-38	131
MS.gene64425.t1	MTR_2g030895	41.420	169	94	3	38	201	7	175	1.41e-37	129
MS.gene64425.t1	MTR_6g005330	41.711	187	101	4	21	201	28	212	2.17e-37	129
MS.gene64425.t1	MTR_2g030865	40.828	169	95	3	38	201	7	175	2.29e-36	125
MS.gene64425.t1	MTR_2g072560	54.464	112	49	2	2	113	6	115	2.39e-36	124
MS.gene64425.t1	MTR_4g027415	49.606	127	62	2	15	141	18	142	6.97e-36	123
MS.gene64425.t1	MTR_1g085500	36.612	183	114	1	21	201	29	211	9.62e-35	122
MS.gene64425.t1	MTR_5g036570	36.508	189	117	2	21	206	33	221	4.04e-34	121
MS.gene64425.t1	MTR_6g005350	38.172	186	108	4	21	201	28	211	2.36e-31	114
MS.gene64425.t1	MTR_6g005380	38.095	189	108	4	12	193	19	205	2.43e-31	114
MS.gene64425.t1	MTR_3g019610	53.398	103	46	2	2	104	6	106	8.24e-31	109
MS.gene64425.t1	MTR_2g031300	50.943	106	50	1	38	141	7	112	4.23e-30	108
MS.gene64425.t1	MTR_8g007300	34.831	178	103	5	1	167	4	179	1.77e-28	106
MS.gene64425.t1	MTR_3g096330	33.838	198	120	3	4	191	558	754	7.14e-28	110
MS.gene64425.t1	MTR_2g086630	34.426	183	105	4	23	201	30	201	1.54e-24	96.3
MS.gene64425.t1	MTR_2g086620	32.683	205	122	5	1	201	9	201	2.33e-24	95.5
MS.gene64425.t1	MTR_2g086640	32.195	205	123	5	1	201	8	200	9.82e-24	94.0
MS.gene64425.t1	MTR_4g052780	33.333	129	85	1	74	201	74	202	1.16e-17	78.2
MS.gene64425.t1	MTR_4g052770	33.333	129	85	1	74	201	74	202	1.59e-17	77.8
MS.gene64425.t1	MTR_2g096110	30.709	127	72	2	41	166	6	117	6.53e-17	74.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64425.t1	AT5G20630	65.385	208	71	1	1	207	3	210	3.28e-93	271
MS.gene64425.t1	AT1G72610	63.942	208	75	0	1	208	1	208	8.60e-87	255
MS.gene64425.t1	AT5G39160	43.684	190	99	3	21	205	30	216	4.13e-44	147
MS.gene64425.t1	AT3G04200	45.833	192	96	5	21	205	33	223	1.09e-43	146
MS.gene64425.t1	AT5G39190	43.979	191	101	3	21	205	30	220	2.53e-43	145
MS.gene64425.t1	AT5G39160	43.979	191	101	3	21	205	30	220	2.67e-43	145
MS.gene64425.t1	AT5G39130	43.455	191	102	3	21	205	30	220	7.13e-43	144
MS.gene64425.t1	AT1G18980	40.887	203	115	3	1	201	16	215	9.19e-41	138
MS.gene64425.t1	AT1G18970	40.394	203	115	3	3	201	15	215	2.45e-40	137
MS.gene64425.t1	AT5G39110	41.799	189	103	3	21	202	30	218	6.28e-40	136
MS.gene64425.t1	AT3G62020	37.073	205	119	3	7	201	8	212	1.20e-39	135
MS.gene64425.t1	AT3G05950	42.781	187	101	3	21	201	32	218	2.65e-39	135
MS.gene64425.t1	AT1G02335	35.122	205	131	1	3	205	12	216	3.90e-39	134
MS.gene64425.t1	AT5G38960	41.327	196	100	5	21	205	32	223	4.54e-39	134
MS.gene64425.t1	AT5G39120	38.498	213	120	4	1	202	5	217	7.14e-39	133
MS.gene64425.t1	AT3G04150	41.026	195	107	4	21	208	32	225	3.29e-38	132
MS.gene64425.t1	AT5G39150	38.028	213	121	4	1	202	5	217	6.71e-38	131
MS.gene64425.t1	AT5G39180	38.028	213	121	4	1	202	5	217	1.10e-37	130
MS.gene64425.t1	AT3G04150	41.341	179	100	2	35	208	60	238	1.60e-37	130
MS.gene64425.t1	AT5G26700	36.634	202	122	3	1	201	10	206	3.68e-37	129
MS.gene64425.t1	AT5G61750	44.361	133	74	0	35	167	41	173	7.14e-37	128
MS.gene64425.t1	AT4G14630	39.894	188	106	3	21	201	32	219	8.10e-37	128
MS.gene64425.t1	AT1G09560	43.503	177	98	1	6	182	17	191	1.24e-36	127
MS.gene64425.t1	AT5G39190	44.304	158	83	2	53	205	41	198	1.76e-36	127
MS.gene64425.t1	AT5G39160	44.304	158	83	2	53	205	41	198	2.12e-36	126
MS.gene64425.t1	AT5G38940	45.695	151	78	2	21	167	32	182	3.59e-36	126
MS.gene64425.t1	AT3G04190	39.791	191	107	4	21	204	32	221	4.07e-36	126
MS.gene64425.t1	AT3G62020	38.235	170	102	2	35	201	14	183	4.18e-36	125
MS.gene64425.t1	AT5G38940	45.695	151	78	2	21	167	56	206	5.28e-36	127
MS.gene64425.t1	AT3G04180	41.146	192	103	6	21	204	32	221	5.55e-36	126
MS.gene64425.t1	AT3G05930	38.278	209	121	3	2	202	5	213	7.64e-36	125
MS.gene64425.t1	AT5G38930	40.107	187	106	3	21	201	32	218	1.76e-35	125
MS.gene64425.t1	AT3G10080	38.857	175	101	2	19	188	30	203	3.38e-35	124
MS.gene64425.t1	AT3G10080	38.857	175	101	2	19	188	101	274	1.07e-34	125
MS.gene64425.t1	AT5G38910	44.693	179	87	5	12	180	17	193	6.75e-34	120
MS.gene64425.t1	AT3G04170	39.791	191	104	5	21	204	32	218	2.38e-33	119
MS.gene64425.t1	AT1G10460	37.500	176	106	3	35	206	42	217	5.53e-32	115
MS.gene64425.t1	AT1G74820	35.897	195	121	2	9	201	30	222	4.80e-31	113
MS.gene64425.t1	AT5G38910	34.463	177	71	5	12	180	17	156	8.32e-15	70.1

Find 51 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAGAACCACCAACATTAATT+TGG	0.165752	2.4:-48609836	None:intergenic
TAATCCTCGTTTACAGATAT+TGG	0.259615	2.4:+48609890	MS.gene64425:CDS
GAAGTATTGGTTGTTATTAA+AGG	0.278056	2.4:+48609714	MS.gene64425:CDS
AAGAAGCTTAAGGGTGTTCT+TGG	0.328993	2.4:+48609987	MS.gene64425:CDS
ATAATGTTTGTGGTGTTTCC+AGG	0.357728	2.4:-48609564	None:intergenic
GAATATCCAGCTGGGCCATT+TGG	0.360878	2.4:-48609483	None:intergenic
AAGGGTGTTCTTGGAGGTAG+TGG	0.422600	2.4:+48609996	MS.gene64425:CDS
TCACAATTTCCCGGTGTAAA+CGG	0.422953	2.4:+48609615	MS.gene64425:CDS
CCCCGGTGCTTCGGAAGTAT+TGG	0.434736	2.4:+48609701	MS.gene64425:CDS
TTTCCCGGTGTAAACGGTCT+TGG	0.446321	2.4:+48609621	MS.gene64425:CDS
AATGCACCAAATGGCCCAGC+TGG	0.454211	2.4:+48609477	MS.gene64425:CDS
CATTTCCAAATTAATGTTGG+TGG	0.455196	2.4:+48609831	MS.gene64425:CDS
AACCAATACTTCCGAAGCAC+CGG	0.468211	2.4:-48609703	None:intergenic
TCACACACACCCCGGTGCTT+CGG	0.471434	2.4:+48609692	MS.gene64425:CDS
TGGTGTTTCCAGGTGCGGCT+AGG	0.473117	2.4:-48609554	None:intergenic
TTCCAGGTGCGGCTAGGCCG+TGG	0.474288	2.4:-48609548	None:intergenic
TACCACGGCCTAGCCGCACC+TGG	0.482390	2.4:+48609546	MS.gene64425:CDS
CTACTACGGATGCATGAGAA+AGG	0.482801	2.4:-48609434	None:intergenic
GGCTATATCTAAACGCGCCA+TGG	0.483268	2.4:-48609649	None:intergenic
GCATTAGACTCACAATTTCC+CGG	0.483529	2.4:+48609606	MS.gene64425:CDS
AAGCTTAAGGGTGTTCTTGG+AGG	0.489760	2.4:+48609990	MS.gene64425:CDS
TGCTGTAGTGAAGAAGCTTA+AGG	0.492145	2.4:+48609977	MS.gene64425:CDS
ATTAAAGGAACAATCAGTGC+TGG	0.495019	2.4:+48609729	MS.gene64425:CDS
AGCGGCTTTGATAATGTTTG+TGG	0.500309	2.4:-48609574	None:intergenic
GCTGTAGTGAAGAAGCTTAA+GGG	0.505860	2.4:+48609978	MS.gene64425:CDS
GGTTTGCAAGAATATCCAGC+TGG	0.520856	2.4:-48609492	None:intergenic
ACCAATACTTCCGAAGCACC+GGG	0.531532	2.4:-48609702	None:intergenic
GCAGATTACAATGCACCAAA+TGG	0.532463	2.4:+48609468	MS.gene64425:CDS
TACTACGGATGCATGAGAAA+GGG	0.545226	2.4:-48609433	None:intergenic
CGTTTAGATATAGCCGTTGG+TGG	0.560060	2.4:+48609657	MS.gene64425:CDS
AAGGTAAACAACATTGTCAG+AGG	0.569703	2.4:-48609760	None:intergenic
TTGCATTTCCAAATTAATGT+TGG	0.570619	2.4:+48609828	MS.gene64425:CDS
ATTCCAAGACCGTTTACACC+GGG	0.600502	2.4:-48609624	None:intergenic
TGGAAATGCAACAATCCTTG+TGG	0.601071	2.4:-48609816	None:intergenic
GTTTGTGGTGTTTCCAGGTG+CGG	0.608586	2.4:-48609559	None:intergenic
TACCTTAAAACACTTCACAA+AGG	0.608858	2.4:+48609777	MS.gene64425:CDS
TGCTACGCAGAAATCTACTA+CGG	0.612239	2.4:-48609448	None:intergenic
GATTCCAAGACCGTTTACAC+CGG	0.616073	2.4:-48609625	None:intergenic
GCACCGGGGTGTGTGTGAAG+TGG	0.618194	2.4:-48609687	None:intergenic
GTGGTAGACGAAGTCATTCG+CGG	0.625632	2.4:-48609529	None:intergenic
AAACGGTCTTGGAATCTCCA+TGG	0.625728	2.4:+48609632	MS.gene64425:CDS
AAGTGGAATAACACCACCAA+CGG	0.630116	2.4:-48609670	None:intergenic
GTCTAATGCGGTGGTAACAG+CGG	0.634535	2.4:-48609592	None:intergenic
GTTTGCAAGAATATCCAGCT+GGG	0.638814	2.4:-48609491	None:intergenic
ATTCCACTTCACACACACCC+CGG	0.641125	2.4:+48609684	MS.gene64425:CDS
GCGCGTTTAGATATAGCCGT+TGG	0.647288	2.4:+48609654	MS.gene64425:CDS
GGGAAATTGTGAGTCTAATG+CGG	0.678656	2.4:-48609604	None:intergenic
AAATTGTGAGTCTAATGCGG+TGG	0.693385	2.4:-48609601	None:intergenic
TAATCCAATATCTGTAAACG+AGG	0.700438	2.4:-48609894	None:intergenic
GCGAATGACTTCGTCTACCA+CGG	0.705558	2.4:+48609531	MS.gene64425:CDS
CCAATACTTCCGAAGCACCG+GGG	0.761220	2.4:-48609701	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTGCATTTCCAAATTAATGT+TGG	+	chr2.4:48609828-48609847	MS.gene64425:CDS	25.0%
!!!	GAAGTATTGGTTGTTATTAA+AGG	+	chr2.4:48609714-48609733	MS.gene64425:CDS	25.0%
	CATTTCCAAATTAATGTTGG+TGG	+	chr2.4:48609831-48609850	MS.gene64425:CDS	30.0%
	TAATCCAATATCTGTAAACG+AGG	-	chr2.4:48609897-48609916	None:intergenic	30.0%
	TAATCCTCGTTTACAGATAT+TGG	+	chr2.4:48609890-48609909	MS.gene64425:CDS	30.0%
	TACCTTAAAACACTTCACAA+AGG	+	chr2.4:48609777-48609796	MS.gene64425:CDS	30.0%
	TAGAACCACCAACATTAATT+TGG	-	chr2.4:48609839-48609858	None:intergenic	30.0%
!	GATGTTATGATTTTTCCACA+AGG	+	chr2.4:48609801-48609820	MS.gene64425:CDS	30.0%
!!	GTTTTAGTAATCAACTCAGT+TGG	-	chr2.4:48609942-48609961	None:intergenic	30.0%
	AAGGTAAACAACATTGTCAG+AGG	-	chr2.4:48609763-48609782	None:intergenic	35.0%
	ATAATGTTTGTGGTGTTTCC+AGG	-	chr2.4:48609567-48609586	None:intergenic	35.0%
	ATTAAAGGAACAATCAGTGC+TGG	+	chr2.4:48609729-48609748	MS.gene64425:CDS	35.0%
!!	CACCTTTGTGAAGTGTTTTA+AGG	-	chr2.4:48609782-48609801	None:intergenic	35.0%
	AAATTGTGAGTCTAATGCGG+TGG	-	chr2.4:48609604-48609623	None:intergenic	40.0%
	AAGTGGAATAACACCACCAA+CGG	-	chr2.4:48609673-48609692	None:intergenic	40.0%
	GCAGATTACAATGCACCAAA+TGG	+	chr2.4:48609468-48609487	MS.gene64425:CDS	40.0%
	GCATTAGACTCACAATTTCC+CGG	+	chr2.4:48609606-48609625	MS.gene64425:CDS	40.0%
	GCTGTAGTGAAGAAGCTTAA+GGG	+	chr2.4:48609978-48609997	MS.gene64425:CDS	40.0%
	GGGAAATTGTGAGTCTAATG+CGG	-	chr2.4:48609607-48609626	None:intergenic	40.0%
	GTTTGCAAGAATATCCAGCT+GGG	-	chr2.4:48609494-48609513	None:intergenic	40.0%
	TACTACGGATGCATGAGAAA+GGG	-	chr2.4:48609436-48609455	None:intergenic	40.0%
	TCACAATTTCCCGGTGTAAA+CGG	+	chr2.4:48609615-48609634	MS.gene64425:CDS	40.0%
	TGCTACGCAGAAATCTACTA+CGG	-	chr2.4:48609451-48609470	None:intergenic	40.0%
	TGCTGTAGTGAAGAAGCTTA+AGG	+	chr2.4:48609977-48609996	MS.gene64425:CDS	40.0%
	TGGAAATGCAACAATCCTTG+TGG	-	chr2.4:48609819-48609838	None:intergenic	40.0%
!!	AAGAAGCTTAAGGGTGTTCT+TGG	+	chr2.4:48609987-48610006	MS.gene64425:CDS	40.0%
!!	AGCGGCTTTGATAATGTTTG+TGG	-	chr2.4:48609577-48609596	None:intergenic	40.0%
!!!	TCATTCGCGGTTACTTTTGA+TGG	-	chr2.4:48609519-48609538	None:intergenic	40.0%
	AAACGGTCTTGGAATCTCCA+TGG	+	chr2.4:48609632-48609651	MS.gene64425:CDS	45.0%
	AACCAATACTTCCGAAGCAC+CGG	-	chr2.4:48609706-48609725	None:intergenic	45.0%
	ATTCCAAGACCGTTTACACC+GGG	-	chr2.4:48609627-48609646	None:intergenic	45.0%
	CGTTTAGATATAGCCGTTGG+TGG	+	chr2.4:48609657-48609676	MS.gene64425:CDS	45.0%
	CTACTACGGATGCATGAGAA+AGG	-	chr2.4:48609437-48609456	None:intergenic	45.0%
	GATTCCAAGACCGTTTACAC+CGG	-	chr2.4:48609628-48609647	None:intergenic	45.0%
	GGTTTGCAAGAATATCCAGC+TGG	-	chr2.4:48609495-48609514	None:intergenic	45.0%
!!	AAGCTTAAGGGTGTTCTTGG+AGG	+	chr2.4:48609990-48610009	MS.gene64425:CDS	45.0%
!!!	TTCGCGGTTACTTTTGATGG+TGG	-	chr2.4:48609516-48609535	None:intergenic	45.0%
	ATTCCACTTCACACACACCC+CGG	+	chr2.4:48609684-48609703	MS.gene64425:CDS	50.0%
	GAATATCCAGCTGGGCCATT+TGG	-	chr2.4:48609486-48609505	None:intergenic	50.0%
	GCGAATGACTTCGTCTACCA+CGG	+	chr2.4:48609531-48609550	MS.gene64425:CDS	50.0%
	GCGCGTTTAGATATAGCCGT+TGG	+	chr2.4:48609654-48609673	MS.gene64425:CDS	50.0%
	GGCTATATCTAAACGCGCCA+TGG	-	chr2.4:48609652-48609671	None:intergenic	50.0%
	GTCTAATGCGGTGGTAACAG+CGG	-	chr2.4:48609595-48609614	None:intergenic	50.0%
	GTGGTAGACGAAGTCATTCG+CGG	-	chr2.4:48609532-48609551	None:intergenic	50.0%
	GTTTGTGGTGTTTCCAGGTG+CGG	-	chr2.4:48609562-48609581	None:intergenic	50.0%
	TTTCCCGGTGTAAACGGTCT+TGG	+	chr2.4:48609621-48609640	MS.gene64425:CDS	50.0%
!	ACCAATACTTCCGAAGCACC+GGG	-	chr2.4:48609705-48609724	None:intergenic	50.0%
!!	AAGGGTGTTCTTGGAGGTAG+TGG	+	chr2.4:48609996-48610015	MS.gene64425:CDS	50.0%
	AATGCACCAAATGGCCCAGC+TGG	+	chr2.4:48609477-48609496	MS.gene64425:CDS	55.0%
!!	CCAATACTTCCGAAGCACCG+GGG	-	chr2.4:48609704-48609723	None:intergenic	55.0%
	TCACACACACCCCGGTGCTT+CGG	+	chr2.4:48609692-48609711	MS.gene64425:CDS	60.0%
	TGGTGTTTCCAGGTGCGGCT+AGG	-	chr2.4:48609557-48609576	None:intergenic	60.0%
!	CCCCGGTGCTTCGGAAGTAT+TGG	+	chr2.4:48609701-48609720	MS.gene64425:CDS	60.0%
	GCACCGGGGTGTGTGTGAAG+TGG	-	chr2.4:48609690-48609709	None:intergenic	65.0%
	TACCACGGCCTAGCCGCACC+TGG	+	chr2.4:48609546-48609565	MS.gene64425:CDS	70.0%
	TTCCAGGTGCGGCTAGGCCG+TGG	-	chr2.4:48609551-48609570	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	48609396	48610022	48609396	ID=MS.gene64425
chr2.4	mRNA	48609396	48610022	48609396	ID=MS.gene64425.t1;Parent=MS.gene64425
chr2.4	exon	48609396	48610022	48609396	ID=MS.gene64425.t1.exon1;Parent=MS.gene64425.t1
chr2.4	CDS	48609396	48610022	48609396	ID=cds.MS.gene64425.t1;Parent=MS.gene64425.t1

Gene Sequence

>MS.gene64425

ATGATTCTAACTATCTTCTTCATTTTTTCTCTTCTTTCCCTTTCTCATGCATCCGTAGTAGATTTCTGCGTAGCAGATTACAATGCACCAAATGGCCCAGCTGGATATTCTTGCAAACCACCATCAAAAGTAACCGCGAATGACTTCGTCTACCACGGCCTAGCCGCACCTGGAAACACCACAAACATTATCAAAGCCGCTGTTACCACCGCATTAGACTCACAATTTCCCGGTGTAAACGGTCTTGGAATCTCCATGGCGCGTTTAGATATAGCCGTTGGTGGTGTTATTCCACTTCACACACACCCCGGTGCTTCGGAAGTATTGGTTGTTATTAAAGGAACAATCAGTGCTGGTTTTGTTTCCTCTGACAATGTTGTTTACCTTAAAACACTTCACAAAGGTGATGTTATGATTTTTCCACAAGGATTGTTGCATTTCCAAATTAATGTTGGTGGTTCTAATGCACTTACTTTTAATAGCTTTAGTAGTGCTAATCCTCGTTTACAGATATTGGATTACGCTTTGTTTAAAAGCGATTTTCCAACTGAGTTGATTACTAAAACTACTTTTATTGATGCTGCTGTAGTGAAGAAGCTTAAGGGTGTTCTTGGAGGTAGTGGTTAA

Protein sequence

>MS.gene64425.t1

MILTIFFIFSLLSLSHASVVDFCVADYNAPNGPAGYSCKPPSKVTANDFVYHGLAAPGNTTNIIKAAVTTALDSQFPGVNGLGISMARLDIAVGGVIPLHTHPGASEVLVVIKGTISAGFVSSDNVVYLKTLHKGDVMIFPQGLLHFQINVGGSNALTFNSFSSANPRLQILDYALFKSDFPTELITKTTFIDAAVVKKLKGVLGGSG