AlfalfaGEDB | MS.gene64546

M. sativa cultivar XinJiangDaYe / MS.gene64546

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12854.10 PF13041.9 PF13812.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64546.t1	XP_003623067.2	93.7	537	31	1	1	537	1	534	2.10E-157	565.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64546.t1	Q9FFE3	51.6	182	86	2	40	220	37	217	6.3e-46	186.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64546.t1	G7L4F1	93.7	537	31	1	1	537	1	534	1.5e-157	565.8

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene002910	MS.gene64546	PPI
MS.gene006777	MS.gene64546	PPI
MS.gene00859	MS.gene64546	PPI
MS.gene027331	MS.gene64546	PPI
MS.gene32049	MS.gene64546	PPI
MS.gene39989	MS.gene64546	PPI
MS.gene57169	MS.gene64546	PPI
MS.gene64546	MS.gene045185	PPI
MS.gene64546	MS.gene01870	PPI
MS.gene64546	MS.gene049681	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64546.t1	MTR_7g060720	94.413	537	27	1	1	537	1	534	0.0	1048
MS.gene64546.t1	MTR_4g108650	31.557	469	303	9	50	511	5	462	6.31e-61	215
MS.gene64546.t1	MTR_4g108650	26.650	409	291	5	95	500	151	553	5.28e-43	164
MS.gene64546.t1	MTR_4g108650	27.577	388	274	5	128	512	326	709	5.04e-39	152
MS.gene64546.t1	MTR_7g066990	28.131	487	329	11	47	522	5	481	6.84e-54	194
MS.gene64546.t1	MTR_7g066990	25.310	403	249	6	129	488	299	692	5.14e-36	143
MS.gene64546.t1	MTR_7g066990	20.242	331	188	6	128	455	437	694	2.41e-16	82.8
MS.gene64546.t1	MTR_1g068960	29.440	411	273	6	77	484	61	457	1.16e-53	194
MS.gene64546.t1	MTR_1g068960	26.119	402	257	6	129	493	243	641	1.13e-38	151
MS.gene64546.t1	MTR_1g068960	26.456	412	263	6	128	502	277	685	6.99e-38	148

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64546.t1	AT5G16420	55.622	498	216	5	40	535	37	531	0.0	573
MS.gene64546.t1	AT5G16420	55.622	498	216	5	40	535	37	531	0.0	573
MS.gene64546.t1	AT5G16420	55.622	498	216	5	40	535	37	531	0.0	573
MS.gene64546.t1	AT5G16420	55.622	498	216	5	40	535	37	531	0.0	573
MS.gene64546.t1	AT3G53700	27.098	572	335	13	14	510	11	575	1.56e-60	213
MS.gene64546.t1	AT3G53700	28.065	367	262	2	123	488	224	589	6.43e-49	181
MS.gene64546.t1	AT3G53700	26.862	376	270	5	128	500	300	673	4.93e-39	152
MS.gene64546.t1	AT3G53700	26.623	308	213	6	149	449	391	692	4.80e-27	116
MS.gene64546.t1	AT3G09060	30.717	446	288	9	46	482	4	437	3.17e-60	211
MS.gene64546.t1	AT3G09060	28.281	442	291	7	86	522	148	568	4.51e-40	154

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Find 184 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTCTTGAGAGCCCGTAC+AGG	0.564974	7.3:+44321992	None:intergenic
AACTGCGGAGGCTGGATAAG+AGG	0.420235	7.3:+44322083	None:intergenic
AAGATGGCTTGGTAGGTTTG+GGG	0.496408	7.3:+44322148	None:intergenic
AAGATTTGAAGGGAGAGACG+AGG	0.591795	7.3:+44322214	None:intergenic
AAGGTTGGAAATGCTAAGGA+GGG	0.616700	7.3:-44321056	MS.gene64546:CDS
AATCAACAAGGTAGTTTCAC+TGG	0.507596	7.3:-44320928	MS.gene64546:CDS
AATCAAGTTAACCGCTTCTC+CGG	0.467238	7.3:+44321463	None:intergenic
AATGAAACAAGAAATCAACA+AGG	0.757269	7.3:-44320940	MS.gene64546:CDS
AATGAGAGATGAGAGGAGTT+TGG	0.325808	7.3:+44322252	None:intergenic
ACACCATAAGTAACCTCATT+AGG	0.424876	7.3:+44321518	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACCTACGCTATATTAATTGA+CGG	-	chr7.3:44322237-44322256	MS.gene64546:CDS	30.0%
TATGATTCAGGCTTATTGTA+AGG	-	chr7.3:44321723-44321742	MS.gene64546:CDS	30.0%
TATTGTAAGGAGAAGAAATC+CGG	-	chr7.3:44321736-44321755	MS.gene64546:CDS	30.0%
TCAGAATAAGCGTTATAGAT+TGG	-	chr7.3:44321318-44321337	MS.gene64546:CDS	30.0%
TTTAGAGAGTTTAAGGAAGA+TGG	+	chr7.3:44321089-44321108	None:intergenic	30.0%
AATCAACAAGGTAGTTTCAC+TGG	-	chr7.3:44322290-44322309	MS.gene64546:CDS	35.0%
ACACCATAAGTAACCTCATT+AGG	+	chr7.3:44321703-44321722	None:intergenic	35.0%
ACAGTATAACTAGTCACATC+CGG	+	chr7.3:44321598-44321617	None:intergenic	35.0%
AGCGGAATTTAGAGAGTTTA+AGG	+	chr7.3:44321096-44321115	None:intergenic	35.0%
AGGAATGTGTTTGATGAGTT+TGG	-	chr7.3:44321979-44321998	MS.gene64546:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	44320812	44322428	44320812	ID=MS.gene64546
chr7.3	mRNA	44320812	44322428	44320812	ID=MS.gene64546.t1;Parent=MS.gene64546
chr7.3	exon	44320812	44322428	44320812	ID=MS.gene64546.t1.exon1;Parent=MS.gene64546.t1
chr7.3	CDS	44320812	44322428	44320812	ID=cds.MS.gene64546.t1;Parent=MS.gene64546.t1

Gene Sequence

>MS.gene64546

ATGCGTTCTCTCCTTCCCTCTCACCACCACCACCGCAACCCATTCTCCACCGCAATCTCCGCCGCCATCGCCGCCAACACAACAACCACACCTTCGCTCCCAGATTCCTACAAAATCCAACCACCAATCAAACCCTGGCCCCACCGCCTCAACCCCAAACTCCTCTCATCTCTCATTTCCCGCCAACACGACCCTCGTCTCTCCCTTCAAATCTTCCTTCACGCCCAACACCATCACAAACCTCCCTTCTCCCATAACCCCCAAACCTACCAAGCCATCTTCCTTAAACTCTCTAAATTCCGCTGCTTCGGCGAAATTGAGTCCCTCTTATCCAGCCTCCGCAGTTCTCCCCCGCACTGCTGCGGGGAAGAACCACTTGTAACCGTTATTCGCGGTTATGGATTAGCTGGAAAGCCTGTACGGGCTCTCAAGACTTTTTTGAGGATCGAATCGTTTGGGATTCGACCCTCGGTTAGATCAGTTAATGCATTGTTGAATTCTTTGGTTCAGAATAAGCGTTATAGATTGGCTTTTTTGGTGTTTAAGAATTGTGGTGAGAGGTTTAAGGTTTTGCCGAATGTTGTTAGTTGTAATATTTTGCTTAAGGCGTTATGTAAAGGGAATCAGGTTGAGGTTGCTGTTAAGGTTTTGGATGAGATGCCTGGGATGGGGTTGGTTCCTAATGTTGTTAGTTATACTACTGTTTTGGGTGGGTTTGTTTGGAGGGGTGATATGGAGGGTGCTATGAGGGTTTTTCGTGAGGTTTTGGATCGAGGGTGGAGTCCGGATGTGACTAGTTATACTGTTTTGGTGGATGGGTTTTGTAGGTTAGGGAAATTAGTGGATGCGATTAGGGTGATGGATATTATGGAGGAGAACGGGGTTGAGCCTAATGAGGTTACTTATGGTGTTATGATTCAGGCTTATTGTAAGGAGAAGAAATCCGGAGAAGCGGTTAACTTGATTGAGGATATGATTGCGAAGGATTTGGTTGTGGGTTCGGAGTTGTGTTGTAAGGTTGTTGATGTGTTGTGTGAGGAAGGGAATGTTGAGAAGGCTTGTGAGGTGTGGACGATGGTTTCGAGGAAGAATTGTGGCCTCAATGGGGCTGTTGTTGTGAGTACGCTTATACATTGGCTTTGCAAGAAGGGGAAGGTGTTGGAAGCAAGGAATGTGTTTGATGAGTTTGGGAAGGGATCGGTTGCGAGTCTGTTGACATATAATTCATTGATTGCTGGATTGTGTGAGGGAGGAGAGCTTTGTGAGGCGGCGAGGCTGTGGGATGACATGGTGGAAAAGAGTGTCGCTCCTAATGCTTTTACATATAACATGTTGATTAAAGGGTTTTGTAAGGTTGGAAATGCTAAGGAGGGTATCAGAGTTCTGGAGGAGATGTTGGAAAATAGGTGTTTGCCCAACAAATCAACCTACGCTATATTAATTGACGGGATTTTGCTTTCAGGTGGAATGAAACAAGAAATCAACAAGGTAGTTTCACTGGCCATGTCGACTGGAGTTGATGCTGATTTGTGGAATATTTTTGTAAAACCTGTCATGGGTAATGATGATGGTGGTGAAACTGAACTGGATAGGATATTATTAGAGAATGCACTATGA

Protein sequence

>MS.gene64546.t1

MRSLLPSHHHHRNPFSTAISAAIAANTTTTPSLPDSYKIQPPIKPWPHRLNPKLLSSLISRQHDPRLSLQIFLHAQHHHKPPFSHNPQTYQAIFLKLSKFRCFGEIESLLSSLRSSPPHCCGEEPLVTVIRGYGLAGKPVRALKTFLRIESFGIRPSVRSVNALLNSLVQNKRYRLAFLVFKNCGERFKVLPNVVSCNILLKALCKGNQVEVAVKVLDEMPGMGLVPNVVSYTTVLGGFVWRGDMEGAMRVFREVLDRGWSPDVTSYTVLVDGFCRLGKLVDAIRVMDIMEENGVEPNEVTYGVMIQAYCKEKKSGEAVNLIEDMIAKDLVVGSELCCKVVDVLCEEGNVEKACEVWTMVSRKNCGLNGAVVVSTLIHWLCKKGKVLEARNVFDEFGKGSVASLLTYNSLIAGLCEGGELCEAARLWDDMVEKSVAPNAFTYNMLIKGFCKVGNAKEGIRVLEEMLENRCLPNKSTYAILIDGILLSGGMKQEINKVVSLAMSTGVDADLWNIFVKPVMGNDDGGETELDRILLENAL