AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene008350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008350.t1 MTR_3g064420 98.110 582 10 1 1 581 1 582 0.0 1189
MS.gene008350.t1 MTR_8g074040 50.631 555 258 4 1 553 43 583 0.0 602
MS.gene008350.t1 MTR_5g076240 53.286 563 233 8 1 551 1 545 0.0 590
MS.gene008350.t1 MTR_5g008800 50.271 553 261 3 1 553 1 539 0.0 587
MS.gene008350.t1 MTR_4g121600 51.971 558 253 8 1 551 7 556 0.0 578
MS.gene008350.t1 MTR_2g044180 51.254 558 257 7 1 551 7 556 0.0 575
MS.gene008350.t1 MTR_5g076220 51.964 560 250 8 1 553 1 548 0.0 555
MS.gene008350.t1 MTR_5g008820 47.473 554 269 5 1 551 1 535 0.0 552
MS.gene008350.t1 MTR_5g076200 50.538 558 257 7 1 551 1 546 0.0 542
MS.gene008350.t1 MTR_0161s0050 49.113 564 258 6 1 551 1 548 0.0 531
MS.gene008350.t1 MTR_8g074040 51.357 479 217 4 77 553 1 465 0.0 527
MS.gene008350.t1 MTR_8g074120 49.693 489 230 4 67 553 20 494 1.24e-176 509
MS.gene008350.t1 MTR_2g044180 50.740 473 218 7 86 551 1 465 9.36e-165 478
MS.gene008350.t1 MTR_5g023730 41.367 556 301 11 3 551 4 541 4.71e-141 420
MS.gene008350.t1 MTR_0803s0020 31.354 539 331 11 14 546 235 740 7.00e-64 227
MS.gene008350.t1 MTR_2g105570 29.205 541 334 16 25 549 37 544 2.41e-50 182
MS.gene008350.t1 MTR_4g057730 26.364 550 358 13 14 552 27 540 4.17e-46 171
MS.gene008350.t1 MTR_5g007640 28.205 546 342 15 18 549 73 582 1.27e-44 167
MS.gene008350.t1 MTR_2g095510 27.667 553 344 15 14 552 27 537 2.18e-43 163
MS.gene008350.t1 MTR_8g039720 25.981 535 326 14 37 546 62 551 2.39e-42 160
MS.gene008350.t1 MTR_3g035130 28.442 552 339 14 15 554 5 512 1.27e-41 157
MS.gene008350.t1 MTR_1g026310 26.326 528 331 13 37 546 65 552 3.36e-41 157
MS.gene008350.t1 MTR_3g460770 26.306 555 334 17 19 549 33 536 6.10e-40 153
MS.gene008350.t1 MTR_1g063110 26.825 548 351 16 14 546 28 540 1.08e-39 152
MS.gene008350.t1 MTR_4g077900 27.132 516 311 14 52 547 1 471 3.92e-39 150
MS.gene008350.t1 MTR_4g005750 28.312 551 334 17 25 556 36 544 5.22e-39 150
MS.gene008350.t1 MTR_4g128337 26.691 547 334 16 25 549 42 543 4.94e-38 148
MS.gene008350.t1 MTR_1569s0010 32.249 369 213 11 192 548 186 529 6.20e-38 147
MS.gene008350.t1 MTR_1g063120 26.259 556 348 16 14 546 28 544 9.28e-37 144
MS.gene008350.t1 MTR_5g027500 26.397 519 327 14 49 547 78 561 1.05e-35 141
MS.gene008350.t1 MTR_5g027480 26.742 531 324 17 36 547 64 548 1.67e-34 137
MS.gene008350.t1 MTR_4g057730 25.581 473 312 12 14 475 27 470 3.43e-34 135
MS.gene008350.t1 MTR_1g090737 25.898 529 335 15 43 556 82 568 9.40e-34 135
MS.gene008350.t1 MTR_3g035130 27.812 489 309 11 15 492 5 460 1.76e-32 130
MS.gene008350.t1 MTR_3g088880 28.261 368 222 8 194 546 208 548 2.65e-31 128
MS.gene008350.t1 MTR_1g073180 24.470 519 335 9 39 549 60 529 2.78e-31 128
MS.gene008350.t1 MTR_3g088870 27.945 365 230 7 194 546 207 550 4.49e-31 127
MS.gene008350.t1 MTR_2g030240 25.092 542 345 17 39 547 27 540 5.73e-31 127
MS.gene008350.t1 MTR_4g011790 27.226 393 245 11 179 547 217 592 6.91e-30 124
MS.gene008350.t1 MTR_4g011790 27.157 394 246 11 179 548 217 593 1.11e-29 124
MS.gene008350.t1 MTR_1054s0020 25.836 538 337 18 27 551 281 769 6.40e-29 122
MS.gene008350.t1 MTR_4g013210 27.551 392 228 14 189 547 71 439 1.29e-28 119
MS.gene008350.t1 MTR_2g030240 27.456 397 253 14 180 550 62 449 1.94e-28 119
MS.gene008350.t1 MTR_4g011790 26.185 401 248 10 179 547 217 601 4.38e-28 119
MS.gene008350.t1 MTR_4g057730 24.944 445 294 12 14 447 27 442 1.07e-27 116
MS.gene008350.t1 MTR_1586s0010 26.075 372 246 12 39 400 69 421 1.53e-26 112
MS.gene008350.t1 MTR_1g073180 23.770 488 315 9 39 518 82 520 1.68e-25 110
MS.gene008350.t1 MTR_2g095510 26.786 448 279 14 14 447 27 439 5.76e-25 108
MS.gene008350.t1 MTR_4g013210 26.842 380 205 15 189 547 71 398 1.19e-24 107
MS.gene008350.t1 MTR_4g013210 26.842 380 205 15 189 547 223 550 6.66e-24 106
MS.gene008350.t1 MTR_4g013210 27.446 368 211 14 189 523 223 567 3.73e-22 100
MS.gene008350.t1 MTR_7g084850 24.270 548 348 19 40 550 203 720 1.04e-20 97.1
MS.gene008350.t1 MTR_1g086600 24.593 492 283 19 41 469 100 566 6.29e-20 94.4
MS.gene008350.t1 MTR_8g099420 23.838 495 267 21 41 462 81 538 2.18e-17 86.3
MS.gene008350.t1 MTR_1g026470 26.646 319 198 9 37 339 65 363 1.60e-16 82.4
MS.gene008350.t1 MTR_1g026430 26.332 319 199 9 37 339 65 363 1.91e-16 82.0
MS.gene008350.t1 MTR_3g087980 25.408 429 218 16 141 484 197 608 1.48e-15 80.5
MS.gene008350.t1 MTR_7g109830 29.439 214 137 6 259 469 340 542 4.21e-14 75.9
MS.gene008350.t1 MTR_5g009350 21.497 521 292 18 41 480 80 564 2.18e-13 73.6
MS.gene008350.t1 MTR_5g009350 21.497 521 292 18 41 480 80 564 2.88e-13 73.2
MS.gene008350.t1 MTR_5g009350 21.538 520 297 18 41 488 80 560 3.04e-13 72.8
MS.gene008350.t1 MTR_5g009350 21.457 508 291 17 41 476 80 551 3.30e-13 72.8
MS.gene008350.t1 MTR_1g034360 20.492 488 307 14 40 469 79 543 1.71e-12 70.9
MS.gene008350.t1 MTR_1g030290 32.948 173 94 6 306 469 424 583 8.06e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008350.t1 AT1G65880 61.754 570 201 5 1 560 1 563 0.0 715
MS.gene008350.t1 AT1G65890 60.900 578 213 5 1 570 1 573 0.0 714
MS.gene008350.t1 AT1G66120 58.864 581 224 4 1 575 1 572 0.0 703
MS.gene008350.t1 AT1G68270 53.509 570 217 7 1 560 1 532 0.0 584
MS.gene008350.t1 AT1G20560 50.265 567 264 4 1 563 3 555 0.0 581
MS.gene008350.t1 AT3G16910 52.330 558 252 8 1 551 9 559 0.0 567
MS.gene008350.t1 AT5G16340 49.104 558 261 6 1 551 1 542 0.0 562
MS.gene008350.t1 AT5G16370 48.566 558 264 5 1 551 1 542 0.0 555
MS.gene008350.t1 AT1G21530 50.000 550 260 7 9 553 9 548 0.0 550
MS.gene008350.t1 AT2G17650 49.107 560 265 4 1 550 48 597 0.0 550
MS.gene008350.t1 AT1G21530 50.000 550 258 8 9 553 9 546 0.0 543
MS.gene008350.t1 AT1G21540 49.097 554 260 8 9 553 9 549 0.0 536
MS.gene008350.t1 AT1G75960 48.474 557 266 5 1 551 1 542 0.0 533
MS.gene008350.t1 AT1G77240 47.101 552 269 8 9 553 9 544 0.0 524
MS.gene008350.t1 AT1G20560 50.102 491 227 4 77 563 1 477 2.85e-172 497
MS.gene008350.t1 AT1G76290 45.324 556 278 8 1 551 1 535 1.17e-155 457
MS.gene008350.t1 AT3G21240 28.872 523 328 13 37 549 61 549 9.87e-49 178
MS.gene008350.t1 AT4G05160 26.860 551 350 13 17 552 30 542 9.59e-45 167
MS.gene008350.t1 AT1G20510 27.687 549 329 20 18 546 36 536 2.82e-44 166
MS.gene008350.t1 AT1G51680 29.015 548 338 16 19 549 43 556 1.37e-43 164
MS.gene008350.t1 AT1G20500 25.229 547 345 15 18 546 42 542 6.48e-43 162
MS.gene008350.t1 AT4G19010 26.642 533 336 14 31 547 62 555 1.41e-42 161
MS.gene008350.t1 AT3G21230 28.653 527 328 12 37 549 71 563 1.24e-41 158
MS.gene008350.t1 AT1G20480 27.290 524 329 16 37 546 72 557 1.75e-41 158
MS.gene008350.t1 AT1G65060 27.737 548 340 18 18 549 52 559 8.07e-41 156
MS.gene008350.t1 AT5G63380 26.190 546 326 16 17 546 66 550 1.26e-39 152
MS.gene008350.t1 AT3G48990 27.969 522 309 14 40 543 31 503 1.89e-38 149
MS.gene008350.t1 AT5G38120 27.156 545 336 17 18 546 43 542 3.70e-38 148
MS.gene008350.t1 AT1G51680 28.433 517 319 16 19 518 43 525 2.66e-37 145
MS.gene008350.t1 AT3G21240 28.444 450 285 12 37 476 61 483 1.09e-36 143
MS.gene008350.t1 AT3G21240 28.444 450 285 12 37 476 61 483 1.09e-36 143
MS.gene008350.t1 AT1G20510 28.033 478 283 18 18 475 36 472 1.88e-36 142
MS.gene008350.t1 AT1G30520 25.271 554 372 14 20 549 13 548 9.26e-34 135
MS.gene008350.t1 AT1G30520 25.271 554 372 14 20 549 13 548 9.26e-34 135
MS.gene008350.t1 AT5G38120 27.079 517 316 17 18 518 43 514 1.81e-33 134
MS.gene008350.t1 AT1G62940 26.071 560 340 16 14 549 26 535 1.14e-32 132
MS.gene008350.t1 AT3G21230 27.419 496 312 12 37 518 71 532 1.00e-31 129
MS.gene008350.t1 AT1G51680 28.421 475 296 15 19 476 43 490 1.73e-31 128
MS.gene008350.t1 AT1G20510 27.778 450 264 18 18 447 36 444 4.73e-30 124
MS.gene008350.t1 AT5G38120 26.371 474 295 13 18 475 43 478 4.36e-29 121
MS.gene008350.t1 AT5G38120 26.371 474 295 13 18 475 43 478 4.36e-29 121
MS.gene008350.t1 AT1G65060 27.578 446 274 17 18 447 52 464 6.59e-27 114
MS.gene008350.t1 AT3G21230 27.313 454 289 11 37 476 71 497 1.28e-26 114
MS.gene008350.t1 AT3G16170 25.000 388 240 11 189 547 236 601 1.56e-24 108
MS.gene008350.t1 AT1G20490 25.728 412 263 15 37 436 61 441 3.96e-23 102
MS.gene008350.t1 AT1G20490 25.728 412 263 15 37 436 61 441 3.96e-23 102
MS.gene008350.t1 AT1G30520 24.094 469 318 12 20 471 13 460 7.42e-22 99.4
MS.gene008350.t1 AT1G30520 24.094 469 318 12 20 471 13 460 7.42e-22 99.4
MS.gene008350.t1 AT1G64400 24.903 514 286 22 28 472 65 547 3.26e-21 98.2
MS.gene008350.t1 AT4G23850 24.324 518 281 20 41 480 81 565 2.45e-19 92.4
MS.gene008350.t1 AT5G36880 24.205 566 362 18 25 550 127 665 8.67e-19 90.9
MS.gene008350.t1 AT5G36880 24.205 566 362 18 25 550 127 665 8.67e-19 90.9
MS.gene008350.t1 AT5G36880 24.205 566 362 18 25 550 127 665 8.67e-19 90.9
MS.gene008350.t1 AT5G36880 24.205 566 362 18 25 550 127 665 8.67e-19 90.9
MS.gene008350.t1 AT5G36880 24.205 566 362 18 25 550 177 715 1.05e-18 90.5
MS.gene008350.t1 AT4G14070 21.658 591 321 23 20 501 102 659 2.61e-13 73.6
MS.gene008350.t1 AT1G77590 32.558 172 96 6 306 469 274 433 1.23e-11 67.8
MS.gene008350.t1 AT2G04350 30.418 263 138 13 306 551 449 683 1.41e-11 67.8
MS.gene008350.t1 AT2G04350 30.418 263 138 13 306 551 449 683 1.41e-11 67.8
MS.gene008350.t1 AT1G77590 32.558 172 96 6 306 469 420 579 1.60e-11 67.8
MS.gene008350.t1 AT4G11030 30.808 198 109 10 281 472 372 547 1.63e-11 67.8
MS.gene008350.t1 AT2G47240 28.750 160 101 4 306 463 329 477 5.82e-11 65.9
MS.gene008350.t1 AT2G47240 30.625 160 98 6 306 463 388 536 8.92e-11 65.1
MS.gene008350.t1 AT2G47240 30.625 160 98 6 306 463 388 536 8.92e-11 65.1
MS.gene008350.t1 AT2G47240 30.625 160 98 6 306 463 388 536 8.92e-11 65.1

Find 134 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAATTTACCACACTTTA+TGG 0.126746 3.1:-53814592 MS.gene008350:CDS
TCTAAAGATGTGATTATTTC+AGG 0.185792 3.1:-53814810 MS.gene008350:CDS
TAAAATAATGTTGAAAATAA+TGG 0.193559 3.1:+53815289 None:intergenic
CAAGGACTTTCACCCCATTT+TGG 0.195490 3.1:+53814699 None:intergenic
TGTCACATTATAGAGATTTA+TGG 0.205886 3.1:+53815328 None:intergenic
TATACATAAAGATGGTTATT+TGG 0.207931 3.1:-53814847 MS.gene008350:CDS
ATTTCTTCAGGAAGGTAATT+TGG 0.214710 3.1:+53815611 None:intergenic
ATCTTACCTGTTAAAGTCTT+TGG 0.233560 3.1:+53814537 None:intergenic
TTATGATTGACTTGATTATT+AGG 0.237452 3.1:+53814447 None:intergenic
GCTCTCATCAAGAAAGTTAA+AGG 0.239728 3.1:+53816470 None:intergenic
GATTTACCAAAGACTTTAAC+AGG 0.288395 3.1:-53814543 MS.gene008350:CDS
TTGCAATGCCACATCCAAAA+TGG 0.295462 3.1:-53814713 MS.gene008350:CDS
GAAGCGACGGAGAAAGCTTT+TGG 0.305149 3.1:-53814903 MS.gene008350:CDS
AATAAAACAGGTATCAGTTT+TGG 0.306205 3.1:-53815948 MS.gene008350:intron
TGACTATAAACAACACCTTT+TGG 0.314032 3.1:+53815533 None:intergenic
TCATAATAATGACCAGATTA+TGG 0.314754 3.1:-53814430 MS.gene008350:CDS
GTATAGTTTGCTCGACATTT+TGG 0.327519 3.1:+53816503 None:intergenic
GAAAGTTAAAGGAGTGAGTA+TGG 0.331539 3.1:+53816481 None:intergenic
CATTCATCTTGAATTTCTTC+AGG 0.339619 3.1:+53815599 None:intergenic
TTTAATCCTTGGTTGGGAAA+TGG 0.345052 3.1:-53815483 MS.gene008350:CDS
GGTAATGTCCATAGATAAAC+TGG 0.364652 3.1:+53815452 None:intergenic
TTCCTCCATGAGTAATCTTA+AGG 0.365260 3.1:+53815754 None:intergenic
AAAATAATGTTGAAAATAAT+GGG 0.371892 3.1:+53815290 None:intergenic
GGGGATGGTTGGTTTAGAAC+TGG 0.384146 3.1:-53814882 MS.gene008350:CDS
CGCATTGAGACGGTGGTTGA+TGG 0.387285 3.1:+53815859 None:intergenic
TCATACATTGCTGGAATGTT+TGG 0.387598 3.1:+53815923 None:intergenic
AAGTGAAACGGGTTCCTTCA+TGG 0.397922 3.1:+53816414 None:intergenic
TACCACAAAACACTTAGCTT+TGG 0.397967 3.1:+53814494 None:intergenic
TATGGTCTGACTGAGGCAAC+AGG 0.406278 3.1:-53815143 MS.gene008350:CDS
TGGTAAATTCTTCCTACAAT+AGG 0.411253 3.1:+53814602 None:intergenic
GTAAGAGATGGGAAAACAAT+GGG 0.423096 3.1:-53814978 MS.gene008350:CDS
GATGTGTAATTCAATGTAAT+TGG 0.423792 3.1:+53815572 None:intergenic
TTTGAGTTTAATCCTTGGTT+GGG 0.424831 3.1:-53815489 MS.gene008350:CDS
TTAATCCTTGGTTGGGAAAT+GGG 0.425386 3.1:-53815482 MS.gene008350:CDS
TTTGTGAGTGGCAGAAGAAA+TGG 0.427336 3.1:-53815109 MS.gene008350:CDS
TTACCTATGTTCCATTGTAA+TGG 0.427373 3.1:-53815434 MS.gene008350:CDS
GAGCTCGCCTAGCCGGATTC+CGG 0.428306 3.1:+53815664 None:intergenic
CTTCATGGATGACTGAGGTT+CGG 0.429624 3.1:+53816429 None:intergenic
AAATGCATCTCATACATTGC+TGG 0.433221 3.1:+53815914 None:intergenic
GTTATTTGGAAATTAAAGAT+AGG 0.433555 3.1:-53814833 MS.gene008350:CDS
GACGGTGGTTGATGGTGTTT+AGG 0.435440 3.1:+53815867 None:intergenic
TGCAATGCCACATCCAAAAT+GGG 0.443909 3.1:-53814712 MS.gene008350:CDS
GACGAGATAGAGCCATAATC+TGG 0.444504 3.1:+53814418 None:intergenic
GTAGTGAACCAGTTTATCTA+TGG 0.450766 3.1:-53815460 MS.gene008350:CDS
ACACTTTATGGTACCTAAGA+TGG 0.462947 3.1:-53814580 MS.gene008350:CDS
GAGATTATGTTGAAGGGAAG+TGG 0.465461 3.1:-53814954 MS.gene008350:CDS
AGTTTGTGACCAAGTGAAAC+GGG 0.471117 3.1:+53816403 None:intergenic
CTGAAGAAATTCAAGATGAA+TGG 0.471358 3.1:-53815598 MS.gene008350:CDS
ATGGAGAGTGTGGTAAGAGA+TGG 0.472523 3.1:-53814990 MS.gene008350:CDS
TAAACACTCCCACCGGAATC+CGG 0.473541 3.1:-53815676 MS.gene008350:CDS
CTATGTTCCATTGTAATGGT+TGG 0.477230 3.1:-53815430 MS.gene008350:CDS
CTTTGAGTTTAATCCTTGGT+TGG 0.482972 3.1:-53815490 MS.gene008350:CDS
CGCCTCAGAGTGTCGAAGAA+TGG 0.487931 3.1:+53815823 None:intergenic
AAGTTTGTGACCAAGTGAAA+CGG 0.494196 3.1:+53816402 None:intergenic
TGCCACTCACAAACAAGTGC+TGG 0.499001 3.1:+53815119 None:intergenic
TGGTTTAGAACTGGTGATGT+TGG 0.499794 3.1:-53814873 MS.gene008350:CDS
TCTCCGGTGAATATACTAAC+CGG 0.501022 3.1:-53815233 MS.gene008350:CDS
ATGAGATAATTACCTATTGT+AGG 0.504104 3.1:-53814614 MS.gene008350:CDS
TGGAGAGTGTGGTAAGAGAT+GGG 0.505965 3.1:-53814989 MS.gene008350:CDS
ATTCGTATCAAAAGCAAAAG+AGG 0.506420 3.1:-53815777 MS.gene008350:CDS
AAGGGAAGTGGAATCATGAT+GGG 0.506638 3.1:-53814942 MS.gene008350:CDS
ACAGCAGCTTCTAAAACCTT+AGG 0.514521 3.1:+53814738 None:intergenic
TGCAATATTGAGAGCACGGA+GGG 0.514867 3.1:+53816352 None:intergenic
CATCTTGAATTTCTTCAGGA+AGG 0.517226 3.1:+53815603 None:intergenic
AGGAAGCGAGGCGGCGACAC+CGG 0.518047 3.1:+53816375 None:intergenic
CGTATTCGAGCTCGCCTAGC+CGG 0.519450 3.1:+53815657 None:intergenic
CTTGAGAAAATCGAGCCACT+TGG 0.519790 3.1:-53815185 MS.gene008350:CDS
ATGAAGGAACCCGTTTCACT+TGG 0.520277 3.1:-53816412 MS.gene008350:CDS
AGTACTTTGAGTTTAATCCT+TGG 0.521867 3.1:-53815494 MS.gene008350:CDS
CTCGCCTAGCCGGATTCCGG+TGG 0.524552 3.1:+53815667 None:intergenic
AGAGGCCTTAAGATTACTCA+TGG 0.524889 3.1:-53815759 MS.gene008350:CDS
ATTACATTGAATTACACATC+AGG 0.525480 3.1:-53815569 MS.gene008350:CDS
CGAGCTCGAATACGAGCAAA+TGG 0.527362 3.1:-53815648 MS.gene008350:CDS
TGTTCTTTATAGTCATCCTA+AGG 0.533616 3.1:-53814754 MS.gene008350:CDS
AACCGGTGGAGCACCACCCC+CGG 0.538649 3.1:-53815216 MS.gene008350:CDS
TGTGACACTAAGGAAGAATG+AGG 0.538924 3.1:-53814670 MS.gene008350:CDS
GAAGGGAAGTGGAATCATGA+TGG 0.539328 3.1:-53814943 MS.gene008350:CDS
GTTGGTGTTATACATAAAGA+TGG 0.545404 3.1:-53814855 MS.gene008350:CDS
AAAGAGAGTCATAAAATCTC+CGG 0.548039 3.1:-53815249 MS.gene008350:CDS
TCGAAGAATGGTGGCTATGT+TGG 0.554863 3.1:+53815835 None:intergenic
CAACCATTACAATGGAACAT+AGG 0.559310 3.1:+53815431 None:intergenic
CACTACCCATTTCCCAACCA+AGG 0.560736 3.1:+53815477 None:intergenic
CACGGAGGGAGGAAGCGAGG+CGG 0.564152 3.1:+53816366 None:intergenic
GGTACAACTTCAGAACCAAA+AGG 0.565976 3.1:-53815548 MS.gene008350:CDS
TCTTTGCAATATTGAGAGCA+CGG 0.566149 3.1:+53816348 None:intergenic
CGATGCTGAAAGCGCGACAA+GGG 0.572970 3.1:-53815061 MS.gene008350:CDS
TACGAGCAAATGGTTCATCA+TGG 0.574224 3.1:-53815638 MS.gene008350:CDS
GTAATGGTTGGACATTCACT+TGG 0.575202 3.1:-53815418 MS.gene008350:CDS
CTTGGTCACAAACTTATGAC+CGG 0.576665 3.1:-53816394 MS.gene008350:CDS
CATCTATCACAATCACTAGT+GGG 0.580127 3.1:+53815702 None:intergenic
TCGATGCTGAAAGCGCGACA+AGG 0.582751 3.1:-53815062 MS.gene008350:CDS
GATGGTGTTTAGGACAGCAC+CGG 0.583797 3.1:+53815877 None:intergenic
TAATGGTTGGACATTCACTT+GGG 0.583819 3.1:-53815417 MS.gene008350:CDS
TTTAGGACAGCACCGGCCAT+AGG 0.587665 3.1:+53815884 None:intergenic
GAGCACGGAGGGAGGAAGCG+AGG 0.588763 3.1:+53816363 None:intergenic
TCTTGAAACAATGGAGAGTG+TGG 0.595422 3.1:-53815000 MS.gene008350:CDS
GGCCAGCACTTGTTTGTGAG+TGG 0.595693 3.1:-53815121 MS.gene008350:CDS
GGTGTTGTTTATAGTCATAG+AGG 0.595730 3.1:-53815527 MS.gene008350:CDS
TCATCTATCACAATCACTAG+TGG 0.596057 3.1:+53815701 None:intergenic
TGTGAAGAATCTTGAAACAA+TGG 0.596126 3.1:-53815009 MS.gene008350:CDS
ACTCCCACCGGAATCCGGCT+AGG 0.597247 3.1:-53815671 MS.gene008350:CDS
CGAACCTCAGTCATCCATGA+AGG 0.598539 3.1:-53816428 MS.gene008350:CDS
AAGAGATGGGAAAACAATGG+GGG 0.600740 3.1:-53814976 MS.gene008350:CDS
TTTCTCAAGTAGGGACGCCG+GGG 0.601501 3.1:+53815199 None:intergenic
CTATGTTGGCCGCATTGAGA+CGG 0.602111 3.1:+53815849 None:intergenic
CTCAGAGTGTCGAAGAATGG+TGG 0.608683 3.1:+53815826 None:intergenic
TGAATGTCCAACCATTACAA+TGG 0.613092 3.1:+53815423 None:intergenic
CCACCGGTTAGTATATTCAC+CGG 0.615355 3.1:+53815230 None:intergenic
GGTAAGAGATGGGAAAACAA+TGG 0.619269 3.1:-53814979 MS.gene008350:CDS
ACTTGGGGTGTAGCTGCAAG+AGG 0.623515 3.1:-53815401 MS.gene008350:CDS
TAAGAGATGGGAAAACAATG+GGG 0.626561 3.1:-53814977 MS.gene008350:CDS
GATGCTGAAAGCGCGACAAG+GGG 0.626984 3.1:-53815060 MS.gene008350:CDS
TCGCCTAGCCGGATTCCGGT+GGG 0.628633 3.1:+53815668 None:intergenic
AATATTGAGAGCACGGAGGG+AGG 0.632194 3.1:+53816355 None:intergenic
GATGACATAAACACTCCCAC+CGG 0.634917 3.1:-53815683 MS.gene008350:CDS
CTTAGTGTCACAAAAGCACA+AGG 0.636960 3.1:+53814681 None:intergenic
TTCTCAAGTAGGGACGCCGG+GGG 0.637975 3.1:+53815200 None:intergenic
TGTTGGCCGCATTGAGACGG+TGG 0.638475 3.1:+53815852 None:intergenic
CACCATTCTTCGACACTCTG+AGG 0.640216 3.1:-53815825 MS.gene008350:CDS
AATGGTTGGACATTCACTTG+GGG 0.644019 3.1:-53815416 MS.gene008350:CDS
AAAGATGTGATTATTTCAGG+AGG 0.649768 3.1:-53814807 MS.gene008350:CDS
ATGGTCTGACTGAGGCAACA+GGG 0.656587 3.1:-53815142 MS.gene008350:CDS
TGATTCTTGTTGTGTTACTG+AGG 0.660637 3.1:-53814637 MS.gene008350:CDS
ATCTTAGGTACCATAAAGTG+TGG 0.661614 3.1:+53814582 None:intergenic
GGCCTTAAGATTACTCATGG+AGG 0.667273 3.1:-53815756 MS.gene008350:CDS
CCGGTGAATATACTAACCGG+TGG 0.668788 3.1:-53815230 MS.gene008350:CDS
AGGGAAGTGGAATCATGATG+GGG 0.670807 3.1:-53814941 MS.gene008350:CDS
GACACTAAGGAAGAATGAGG+AGG 0.677688 3.1:-53814667 MS.gene008350:CDS
GGTTCCTTCATGGATGACTG+AGG 0.686748 3.1:+53816424 None:intergenic
GCAATGCCACATCCAAAATG+GGG 0.688593 3.1:-53814711 MS.gene008350:CDS
TGGGGTGTAGCTGCAAGAGG+CGG 0.689296 3.1:-53815398 MS.gene008350:CDS
ACATGCTTATGGTCTGACTG+AGG 0.729298 3.1:-53815150 MS.gene008350:CDS
TTGCAATATTGAGAGCACGG+AGG 0.733139 3.1:+53816351 None:intergenic
CATCAACCACCGTCTCAATG+CGG 0.733801 3.1:-53815858 MS.gene008350:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATGTTGAAAATAAT+GGG + chr3.1:53815641-53815660 None:intergenic 10.0%
!! TAAAATAATGTTGAAAATAA+TGG + chr3.1:53815642-53815661 None:intergenic 10.0%
!!! ATTATTTTCAACATTATTTT+AGG - chr3.1:53815641-53815660 MS.gene008350:CDS 10.0%
!! TATTACAATATTTGATATTG+CGG + chr3.1:53814867-53814886 None:intergenic 15.0%
!! GCGATTTATAAAAACTAAAT+TGG - chr3.1:53814948-53814967 MS.gene008350:CDS 20.0%
!! GTTATTTGGAAATTAAAGAT+AGG - chr3.1:53816095-53816114 MS.gene008350:intron 20.0%
!! TATACATAAAGATGGTTATT+TGG - chr3.1:53816081-53816100 MS.gene008350:intron 20.0%
!!! TGGTTTTTGTTTAATAAAAC+AGG - chr3.1:53814968-53814987 MS.gene008350:CDS 20.0%
!!! TTATGATTGACTTGATTATT+AGG + chr3.1:53816484-53816503 None:intergenic 20.0%
! ATGAGATAATTACCTATTGT+AGG - chr3.1:53816314-53816333 MS.gene008350:intron 25.0%
! ATTACATTGAATTACACATC+AGG - chr3.1:53815359-53815378 MS.gene008350:CDS 25.0%
! CAAAATGCAGTATCAAATAT+TGG + chr3.1:53814843-53814862 None:intergenic 25.0%
! GATGTGTAATTCAATGTAAT+TGG + chr3.1:53815359-53815378 None:intergenic 25.0%
! TCATAATAATGACCAGATTA+TGG - chr3.1:53816498-53816517 MS.gene008350:CDS 25.0%
! TCTAAAGATGTGATTATTTC+AGG - chr3.1:53816118-53816137 MS.gene008350:intron 25.0%
! TGTCACATTATAGAGATTTA+TGG + chr3.1:53815603-53815622 None:intergenic 25.0%
!! AATAAAACAGGTATCAGTTT+TGG - chr3.1:53814980-53814999 MS.gene008350:CDS 25.0%
!! TTTTATGTAACACATGCTTA+TGG - chr3.1:53815767-53815786 MS.gene008350:CDS 25.0%
!!! TTTTAAAGATAAAGAAGCGA+CGG - chr3.1:53816012-53816031 MS.gene008350:intron 25.0%
AAAGAGAGTCATAAAATCTC+CGG - chr3.1:53815679-53815698 MS.gene008350:CDS 30.0%
AAAGATGTGATTATTTCAGG+AGG - chr3.1:53816121-53816140 MS.gene008350:intron 30.0%
ATCTTACCTGTTAAAGTCTT+TGG + chr3.1:53816394-53816413 None:intergenic 30.0%
ATTCGTATCAAAAGCAAAAG+AGG - chr3.1:53815151-53815170 MS.gene008350:CDS 30.0%
ATTTCTTCAGGAAGGTAATT+TGG + chr3.1:53815320-53815339 None:intergenic 30.0%
CATTCATCTTGAATTTCTTC+AGG + chr3.1:53815332-53815351 None:intergenic 30.0%
CTGAAGAAATTCAAGATGAA+TGG - chr3.1:53815330-53815349 MS.gene008350:CDS 30.0%
GAAGAATTTACCACACTTTA+TGG - chr3.1:53816336-53816355 MS.gene008350:intron 30.0%
GATTTACCAAAGACTTTAAC+AGG - chr3.1:53816385-53816404 MS.gene008350:CDS 30.0%
TCCATAAAAACAACCATCTT+AGG + chr3.1:53816364-53816383 None:intergenic 30.0%
TGATATCATATCCTAGATTG+CGG + chr3.1:53814805-53814824 None:intergenic 30.0%
TGGTAAATTCTTCCTACAAT+AGG + chr3.1:53816329-53816348 None:intergenic 30.0%
TGTGAAGAATCTTGAAACAA+TGG - chr3.1:53815919-53815938 MS.gene008350:CDS 30.0%
TGTGTTACATAAAAACCAAG+TGG + chr3.1:53815761-53815780 None:intergenic 30.0%
TGTTCTTTATAGTCATCCTA+AGG - chr3.1:53816174-53816193 MS.gene008350:intron 30.0%
TTACCTATGTTCCATTGTAA+TGG - chr3.1:53815494-53815513 MS.gene008350:CDS 30.0%
! AGTACTTTGAGTTTAATCCT+TGG - chr3.1:53815434-53815453 MS.gene008350:CDS 30.0%
! TGACTATAAACAACACCTTT+TGG + chr3.1:53815398-53815417 None:intergenic 30.0%
! TTTGAGTTTAATCCTTGGTT+GGG - chr3.1:53815439-53815458 MS.gene008350:CDS 30.0%
!! GTTGGTGTTATACATAAAGA+TGG - chr3.1:53816073-53816092 MS.gene008350:intron 30.0%
!!! ACCTAAGATGGTTGTTTTTA+TGG - chr3.1:53816360-53816379 MS.gene008350:CDS 30.0%
!!! TAAGATGGTTGTTTTTATGG+AGG - chr3.1:53816363-53816382 MS.gene008350:CDS 30.0%
AAATGCATCTCATACATTGC+TGG + chr3.1:53815017-53815036 None:intergenic 35.0%
AAGTTTGTGACCAAGTGAAA+CGG + chr3.1:53814529-53814548 None:intergenic 35.0%
ACACTTTATGGTACCTAAGA+TGG - chr3.1:53816348-53816367 MS.gene008350:CDS 35.0%
ATCTTAGGTACCATAAAGTG+TGG + chr3.1:53816349-53816368 None:intergenic 35.0%
CAACCATTACAATGGAACAT+AGG + chr3.1:53815500-53815519 None:intergenic 35.0%
CATCTATCACAATCACTAGT+GGG + chr3.1:53815229-53815248 None:intergenic 35.0%
CATCTTGAATTTCTTCAGGA+AGG + chr3.1:53815328-53815347 None:intergenic 35.0%
CTATGTTCCATTGTAATGGT+TGG - chr3.1:53815498-53815517 MS.gene008350:CDS 35.0%
GAAAGTTAAAGGAGTGAGTA+TGG + chr3.1:53814450-53814469 None:intergenic 35.0%
GCTCTCATCAAGAAAGTTAA+AGG + chr3.1:53814461-53814480 None:intergenic 35.0%
GGTAATGTCCATAGATAAAC+TGG + chr3.1:53815479-53815498 None:intergenic 35.0%
GTAAGAGATGGGAAAACAAT+GGG - chr3.1:53815950-53815969 MS.gene008350:intron 35.0%
GTAGTGAACCAGTTTATCTA+TGG - chr3.1:53815468-53815487 MS.gene008350:CDS 35.0%
TAAGAGATGGGAAAACAATG+GGG - chr3.1:53815951-53815970 MS.gene008350:intron 35.0%
TAATGGTTGGACATTCACTT+GGG - chr3.1:53815511-53815530 MS.gene008350:CDS 35.0%
TACCACAAAACACTTAGCTT+TGG + chr3.1:53816437-53816456 None:intergenic 35.0%
TCATACATTGCTGGAATGTT+TGG + chr3.1:53815008-53815027 None:intergenic 35.0%
TCATCTATCACAATCACTAG+TGG + chr3.1:53815230-53815249 None:intergenic 35.0%
TCTTTGCAATATTGAGAGCA+CGG + chr3.1:53814583-53814602 None:intergenic 35.0%
TGAATGTCCAACCATTACAA+TGG + chr3.1:53815508-53815527 None:intergenic 35.0%
TTAATCCTTGGTTGGGAAAT+GGG - chr3.1:53815446-53815465 MS.gene008350:CDS 35.0%
TTCCTCCATGAGTAATCTTA+AGG + chr3.1:53815177-53815196 None:intergenic 35.0%
TTTAATCCTTGGTTGGGAAA+TGG - chr3.1:53815445-53815464 MS.gene008350:CDS 35.0%
! CTTTGAGTTTAATCCTTGGT+TGG - chr3.1:53815438-53815457 MS.gene008350:CDS 35.0%
! TGATTCTTGTTGTGTTACTG+AGG - chr3.1:53816291-53816310 MS.gene008350:intron 35.0%
! TTTATGACTCTCTTTTCGCT+TGG + chr3.1:53815674-53815693 None:intergenic 35.0%
!! GGTGTTGTTTATAGTCATAG+AGG - chr3.1:53815401-53815420 MS.gene008350:CDS 35.0%
!!! GTATAGTTTGCTCGACATTT+TGG + chr3.1:53814428-53814447 None:intergenic 35.0%
AAGAGATGGGAAAACAATGG+GGG - chr3.1:53815952-53815971 MS.gene008350:intron 40.0%
AAGGGAAGTGGAATCATGAT+GGG - chr3.1:53815986-53816005 MS.gene008350:intron 40.0%
AATGGGGGAGATTATGTTGA+AGG - chr3.1:53815967-53815986 MS.gene008350:intron 40.0%
AATGGTTGGACATTCACTTG+GGG - chr3.1:53815512-53815531 MS.gene008350:CDS 40.0%
AGAGGCCTTAAGATTACTCA+TGG - chr3.1:53815169-53815188 MS.gene008350:CDS 40.0%
AGTTTGTGACCAAGTGAAAC+GGG + chr3.1:53814528-53814547 None:intergenic 40.0%
ATGGGGGAGATTATGTTGAA+GGG - chr3.1:53815968-53815987 MS.gene008350:intron 40.0%
CTTGGTCACAAACTTATGAC+CGG - chr3.1:53814534-53814553 MS.gene008350:CDS 40.0%
GAGATTATGTTGAAGGGAAG+TGG - chr3.1:53815974-53815993 MS.gene008350:intron 40.0%
GGTAAGAGATGGGAAAACAA+TGG - chr3.1:53815949-53815968 MS.gene008350:intron 40.0%
GGTACAACTTCAGAACCAAA+AGG - chr3.1:53815380-53815399 MS.gene008350:CDS 40.0%
GTAATGGTTGGACATTCACT+TGG - chr3.1:53815510-53815529 MS.gene008350:CDS 40.0%
TACGAGCAAATGGTTCATCA+TGG - chr3.1:53815290-53815309 MS.gene008350:CDS 40.0%
TCTCCGGTGAATATACTAAC+CGG - chr3.1:53815695-53815714 MS.gene008350:CDS 40.0%
TCTTGAAACAATGGAGAGTG+TGG - chr3.1:53815928-53815947 MS.gene008350:CDS 40.0%
TGCAATGCCACATCCAAAAT+GGG - chr3.1:53816216-53816235 MS.gene008350:intron 40.0%
TGTGACACTAAGGAAGAATG+AGG - chr3.1:53816258-53816277 MS.gene008350:intron 40.0%
TTGCAATGCCACATCCAAAA+TGG - chr3.1:53816215-53816234 MS.gene008350:intron 40.0%
TTTGTGAGTGGCAGAAGAAA+TGG - chr3.1:53815819-53815838 MS.gene008350:CDS 40.0%
! AGCCAAAGCTAAGTGTTTTG+TGG - chr3.1:53816432-53816451 MS.gene008350:CDS 40.0%
! CTTAGTGTCACAAAAGCACA+AGG + chr3.1:53816250-53816269 None:intergenic 40.0%
! CTTGTGCTTTTGTGACACTA+AGG - chr3.1:53816248-53816267 MS.gene008350:intron 40.0%
! GATGCATTTTGCAGTACCTA+TGG - chr3.1:53815028-53815047 MS.gene008350:CDS 40.0%
! TGGCTCGATTTTCTCAAGTA+GGG + chr3.1:53815741-53815760 None:intergenic 40.0%
! TGGTTTAGAACTGGTGATGT+TGG - chr3.1:53816055-53816074 MS.gene008350:intron 40.0%
!! ACAGCAGCTTCTAAAACCTT+AGG + chr3.1:53816193-53816212 None:intergenic 40.0%
!! AGTTTGCTCGACATTTTGGT+AGG + chr3.1:53814424-53814443 None:intergenic 40.0%
!!! AGCATCGATTGCTCTCTTTT+CGG + chr3.1:53815854-53815873 None:intergenic 40.0%
!!! TAAGGTTTTAGAAGCTGCTG+TGG - chr3.1:53816192-53816211 MS.gene008350:intron 40.0%
ACATGCTTATGGTCTGACTG+AGG - chr3.1:53815778-53815797 MS.gene008350:CDS 45.0%
AGGGAAGTGGAATCATGATG+GGG - chr3.1:53815987-53816006 MS.gene008350:intron 45.0%
ATGAAGGAACCCGTTTCACT+TGG - chr3.1:53814516-53814535 MS.gene008350:CDS 45.0%
CTTCATGGATGACTGAGGTT+CGG + chr3.1:53814502-53814521 None:intergenic 45.0%
CTTGAGAAAATCGAGCCACT+TGG - chr3.1:53815743-53815762 MS.gene008350:CDS 45.0%
GAAGGGAAGTGGAATCATGA+TGG - chr3.1:53815985-53816004 MS.gene008350:intron 45.0%
GACACTAAGGAAGAATGAGG+AGG - chr3.1:53816261-53816280 MS.gene008350:intron 45.0%
GACGAGATAGAGCCATAATC+TGG + chr3.1:53816513-53816532 None:intergenic 45.0%
GATGACATAAACACTCCCAC+CGG - chr3.1:53815245-53815264 MS.gene008350:CDS 45.0%
GCAATGCCACATCCAAAATG+GGG - chr3.1:53816217-53816236 MS.gene008350:intron 45.0%
GGCCTTAAGATTACTCATGG+AGG - chr3.1:53815172-53815191 MS.gene008350:CDS 45.0%
TCGAAGAATGGTGGCTATGT+TGG + chr3.1:53815096-53815115 None:intergenic 45.0%
! AAGCGACGGAGAAAGCTTTT+GGG - chr3.1:53816026-53816045 MS.gene008350:intron 45.0%
! AAGTGAAACGGGTTCCTTCA+TGG + chr3.1:53814517-53814536 None:intergenic 45.0%
! ATGGAGAGTGTGGTAAGAGA+TGG - chr3.1:53815938-53815957 MS.gene008350:CDS 45.0%
! ATTTTCTCAAGTAGGGACGC+CGG + chr3.1:53815734-53815753 None:intergenic 45.0%
! CAAGGACTTTCACCCCATTT+TGG + chr3.1:53816232-53816251 None:intergenic 45.0%
! CATTTTGCAGTACCTATGGC+CGG - chr3.1:53815032-53815051 MS.gene008350:CDS 45.0%
! CCACCGGTTAGTATATTCAC+CGG + chr3.1:53815701-53815720 None:intergenic 45.0%
! CTTTCACCCCATTTTGGATG+TGG + chr3.1:53816226-53816245 None:intergenic 45.0%
! GTGGCTCGATTTTCTCAAGT+AGG + chr3.1:53815742-53815761 None:intergenic 45.0%
! TGCAATATTGAGAGCACGGA+GGG + chr3.1:53814579-53814598 None:intergenic 45.0%
! TGGAGAGTGTGGTAAGAGAT+GGG - chr3.1:53815939-53815958 MS.gene008350:CDS 45.0%
! TTGCAATATTGAGAGCACGG+AGG + chr3.1:53814580-53814599 None:intergenic 45.0%
!! AGAAAGCTTTTGGGGATGGT+TGG - chr3.1:53816035-53816054 MS.gene008350:intron 45.0%
ATGGTCTGACTGAGGCAACA+GGG - chr3.1:53815786-53815805 MS.gene008350:CDS 50.0%
CACCATTCTTCGACACTCTG+AGG - chr3.1:53815103-53815122 MS.gene008350:CDS 50.0%
CACTACCCATTTCCCAACCA+AGG + chr3.1:53815454-53815473 None:intergenic 50.0%
CATCAACCACCGTCTCAATG+CGG - chr3.1:53815070-53815089 MS.gene008350:CDS 50.0%
CCGGTGAATATACTAACCGG+TGG - chr3.1:53815698-53815717 MS.gene008350:CDS 50.0%
CGAACCTCAGTCATCCATGA+AGG - chr3.1:53814500-53814519 MS.gene008350:CDS 50.0%
CGAGCTCGAATACGAGCAAA+TGG - chr3.1:53815280-53815299 MS.gene008350:CDS 50.0%
CTATGTTGGCCGCATTGAGA+CGG + chr3.1:53815082-53815101 None:intergenic 50.0%
GGTTCCTTCATGGATGACTG+AGG + chr3.1:53814507-53814526 None:intergenic 50.0%
TAAACACTCCCACCGGAATC+CGG - chr3.1:53815252-53815271 MS.gene008350:CDS 50.0%
TAGATCAGCGACCGCAATCT+AGG - chr3.1:53814791-53814810 MS.gene008350:CDS 50.0%
TAGATTGCGGTCGCTGATCT+AGG + chr3.1:53814792-53814811 None:intergenic 50.0%
TATGGTCTGACTGAGGCAAC+AGG - chr3.1:53815785-53815804 MS.gene008350:CDS 50.0%
TGCCACTCACAAACAAGTGC+TGG + chr3.1:53815812-53815831 None:intergenic 50.0%
! AATATTGAGAGCACGGAGGG+AGG + chr3.1:53814576-53814595 None:intergenic 50.0%
! AGCGACGGAGAAAGCTTTTG+GGG - chr3.1:53816027-53816046 MS.gene008350:intron 50.0%
! GAAGCGACGGAGAAAGCTTT+TGG - chr3.1:53816025-53816044 MS.gene008350:intron 50.0%
! GATGGTGTTTAGGACAGCAC+CGG + chr3.1:53815054-53815073 None:intergenic 50.0%
! TTTTCTCAAGTAGGGACGCC+GGG + chr3.1:53815733-53815752 None:intergenic 50.0%
!! ACGGAGAAAGCTTTTGGGGA+TGG - chr3.1:53816031-53816050 MS.gene008350:intron 50.0%
!! CTCAGAGTGTCGAAGAATGG+TGG + chr3.1:53815105-53815124 None:intergenic 50.0%
!! GACGGTGGTTGATGGTGTTT+AGG + chr3.1:53815064-53815083 None:intergenic 50.0%
!! GGGGATGGTTGGTTTAGAAC+TGG - chr3.1:53816046-53816065 MS.gene008350:intron 50.0%
ACTTGGGGTGTAGCTGCAAG+AGG - chr3.1:53815527-53815546 MS.gene008350:CDS 55.0%
CGCATTGAGACGGTGGTTGA+TGG + chr3.1:53815072-53815091 None:intergenic 55.0%
GGCCAGCACTTGTTTGTGAG+TGG - chr3.1:53815807-53815826 MS.gene008350:CDS 55.0%
TTTCTCAAGTAGGGACGCCG+GGG + chr3.1:53815732-53815751 None:intergenic 55.0%
! CGATGCTGAAAGCGCGACAA+GGG - chr3.1:53815867-53815886 MS.gene008350:CDS 55.0%
! GATGCTGAAAGCGCGACAAG+GGG - chr3.1:53815868-53815887 MS.gene008350:CDS 55.0%
! TCGATGCTGAAAGCGCGACA+AGG - chr3.1:53815866-53815885 MS.gene008350:CDS 55.0%
!! CGCCTCAGAGTGTCGAAGAA+TGG + chr3.1:53815108-53815127 None:intergenic 55.0%
!! TTTAGGACAGCACCGGCCAT+AGG + chr3.1:53815047-53815066 None:intergenic 55.0%
CGTATTCGAGCTCGCCTAGC+CGG + chr3.1:53815274-53815293 None:intergenic 60.0%
TGGGGTGTAGCTGCAAGAGG+CGG - chr3.1:53815530-53815549 MS.gene008350:CDS 60.0%
TGTTGGCCGCATTGAGACGG+TGG + chr3.1:53815079-53815098 None:intergenic 60.0%
TTCTCAAGTAGGGACGCCGG+GGG + chr3.1:53815731-53815750 None:intergenic 60.0%
ACTCCCACCGGAATCCGGCT+AGG - chr3.1:53815257-53815276 MS.gene008350:CDS 65.0%
GAGCTCGCCTAGCCGGATTC+CGG + chr3.1:53815267-53815286 None:intergenic 65.0%
TCGCCTAGCCGGATTCCGGT+GGG + chr3.1:53815263-53815282 None:intergenic 65.0%
AGGAAGCGAGGCGGCGACAC+CGG + chr3.1:53814556-53814575 None:intergenic 70.0%
CACGGAGGGAGGAAGCGAGG+CGG + chr3.1:53814565-53814584 None:intergenic 70.0%
CTCGCCTAGCCGGATTCCGG+TGG + chr3.1:53815264-53815283 None:intergenic 70.0%
GAGCACGGAGGGAGGAAGCG+AGG + chr3.1:53814568-53814587 None:intergenic 70.0%
TCAAGTAGGGACGCCGGGGG+TGG + chr3.1:53815728-53815747 None:intergenic 70.0%
! AACCGGTGGAGCACCACCCC+CGG - chr3.1:53815712-53815731 MS.gene008350:CDS 70.0%
! CGCCGGGGGTGGTGCTCCAC+CGG + chr3.1:53815717-53815736 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 53814413 53816537 53814413 ID=MS.gene008350
chr3.1 mRNA 53814413 53816537 53814413 ID=MS.gene008350.t1;Parent=MS.gene008350
chr3.1 exon 53816340 53816537 53816340 ID=MS.gene008350.t1.exon1;Parent=MS.gene008350.t1
chr3.1 CDS 53816340 53816537 53816340 ID=cds.MS.gene008350.t1;Parent=MS.gene008350.t1
chr3.1 exon 53814413 53815960 53814413 ID=MS.gene008350.t1.exon2;Parent=MS.gene008350.t1
chr3.1 CDS 53814413 53815960 53814413 ID=cds.MS.gene008350.t1;Parent=MS.gene008350.t1
Gene Sequence

>MS.gene008350

ATGGATAACCTACCAAAATGTCGAGCAAACTATACCATACTCACTCCTTTAACTTTCTTGATGAGAGCTTCTGCATCTTATGCAAACCGAACCTCAGTCATCCATGAAGGAACCCGTTTCACTTGGTCACAAACTTATGACCGGTGTCGCCGCCTCGCTTCCTCCCTCCGTGCTCTCAATATTGCAAAGAATGATGTTGTAAGTTCGTCACTAAATCGTGTTTTTCAAGTAGTACACAAACATCAAAGTATATAAGTATTCAAATCTCAATAAAAATTCATGCATTCATATATCTTGTATTTGATTGCAATTTTTTTGCATTATCGAGTATTGTGACTTAAACGAGATTGCAATTTAAAACGTTACATGTTCATTTCCTAGATCAGCGACCGCAATCTAGGATATGATATCAAGTAAAATTGTATTGCCAATATTTGATACTGCATTTTGTCCGCAATATCAAATATTGTAATAATACACTAACTCAACGTGACTACAATTCAAAATGTTACATACTATTATGATTAAGATTCGCGCGATTTATAAAAACTAAATTGGTTTTTGTTTAATAAAACAGGTATCAGTTTTGGCACCAAACATTCCAGCAATGTATGAGATGCATTTTGCAGTACCTATGGCCGGTGCTGTCCTAAACACCATCAACCACCGTCTCAATGCGGCCAACATAGCCACCATTCTTCGACACTCTGAGGCGAAAGTCTTCTTCGTAGACTACGAATTCGTATCAAAAGCAAAAGAGGCCTTAAGATTACTCATGGAGGAAAAAGATCAAAAAGAACAATACTCATCACTCCCACTAGTGATTGTGATAGATGACATAAACACTCCCACCGGAATCCGGCTAGGCGAGCTCGAATACGAGCAAATGGTTCATCATGGTAATCCAAATTACCTTCCTGAAGAAATTCAAGATGAATGGTCACCAATTACATTGAATTACACATCAGGTACAACTTCAGAACCAAAAGGTGTTGTTTATAGTCATAGAGGAGCTTATTTAAGTACTTTGAGTTTAATCCTTGGTTGGGAAATGGGTAGTGAACCAGTTTATCTATGGACATTACCTATGTTCCATTGTAATGGTTGGACATTCACTTGGGGTGTAGCTGCAAGAGGCGGTACTAATATTTGTATCAGAAACACTGCAGCATCTGATATATATAGAGCCATAAATCTCTATAATGTGACACACATGTGTTGCGCACCCATTATTTTCAACATTATTTTAGGCGCGAAACCAAGCGAAAAGAGAGTCATAAAATCTCCGGTGAATATACTAACCGGTGGAGCACCACCCCCGGCGTCCCTACTTGAGAAAATCGAGCCACTTGGTTTTTATGTAACACATGCTTATGGTCTGACTGAGGCAACAGGGCCAGCACTTGTTTGTGAGTGGCAGAAGAAATGGAATGTGTTGCCGAAAAGAGAGCAATCGATGCTGAAAGCGCGACAAGGGGTTAGTGTTTTGACACTTGCTGATGTTGATGTGAAGAATCTTGAAACAATGGAGAGTGTGGTAAGAGATGGGAAAACAATGGGGGAGATTATGTTGAAGGGAAGTGGAATCATGATGGGGTATTTTAAAGATAAAGAAGCGACGGAGAAAGCTTTTGGGGATGGTTGGTTTAGAACTGGTGATGTTGGTGTTATACATAAAGATGGTTATTTGGAAATTAAAGATAGGTCTAAAGATGTGATTATTTCAGGAGGTGAAAATATTAGTAGTGTTGAAGTTGAAAATGTTCTTTATAGTCATCCTAAGGTTTTAGAAGCTGCTGTGGTTGCAATGCCACATCCAAAATGGGGTGAAAGTCCTTGTGCTTTTGTGACACTAAGGAAGAATGAGGAGGAAAAAAGTGATTCTTGTTGTGTTACTGAGGATGAGATAATTACCTATTGTAGGAAGAATTTACCACACTTTATGGTACCTAAGATGGTTGTTTTTATGGAGGATTTACCAAAGACTTTAACAGGTAAGATTCAGAAATTTGAATTAAGAGCCAAAGCTAAGTGTTTTGTGGTAAATGATGAGAAAAATAAGAAGAAGCCTAATAATCAAGTCAATCATAATAATGACCAGATTATGGCTCTATCTCGTCTTTGA
Protein sequence

>MS.gene008350.t1

MDNLPKCRANYTILTPLTFLMRASASYANRTSVIHEGTRFTWSQTYDRCRRLASSLRALNIAKNDVVSVLAPNIPAMYEMHFAVPMAGAVLNTINHRLNAANIATILRHSEAKVFFVDYEFVSKAKEALRLLMEEKDQKEQYSSLPLVIVIDDINTPTGIRLGELEYEQMVHHGNPNYLPEEIQDEWSPITLNYTSGTTSEPKGVVYSHRGAYLSTLSLILGWEMGSEPVYLWTLPMFHCNGWTFTWGVAARGGTNICIRNTAASDIYRAINLYNVTHMCCAPIIFNIILGAKPSEKRVIKSPVNILTGGAPPPASLLEKIEPLGFYVTHAYGLTEATGPALVCEWQKKWNVLPKREQSMLKARQGVSVLTLADVDVKNLETMESVVRDGKTMGEIMLKGSGIMMGYFKDKEATEKAFGDGWFRTGDVGVIHKDGYLEIKDRSKDVIISGGENISSVEVENVLYSHPKVLEAAVVAMPHPKWGESPCAFVTLRKNEEEKSDSCCVTEDEIITYCRKNLPHFMVPKMVVFMEDLPKTLTGKIQKFELRAKAKCFVVNDEKNKKKPNNQVNHNNDQIMALSRL