Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008350.t1 | XP_003600627.1 | 98.1 | 582 | 10 | 1 | 1 | 581 | 1 | 582 | 0.00E+00 | 1152.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008350.t1 | Q9SS00 | 60.9 | 578 | 213 | 5 | 1 | 570 | 1 | 573 | 1.6e-199 | 697.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008350.t1 | G7J7Y4 | 98.1 | 582 | 10 | 1 | 1 | 581 | 1 | 582 | 0.0e+00 | 1152.1 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| No data available in table | ||||
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene007715 | MS.gene008350 | PPI |
| MS.gene008350 | MS.gene27218 | PPI |
| MS.gene008350 | MS.gene31229 | PPI |
| MS.gene008350 | MS.gene017279 | PPI |
| MS.gene008350 | MS.gene24088 | PPI |
| MS.gene008350 | MS.gene017547 | PPI |
| MS.gene008350 | MS.gene09948 | PPI |
| MS.gene008350 | MS.gene98148 | PPI |
| MS.gene008350 | MS.gene99857 | PPI |
| MS.gene008350 | MS.gene041843 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008350.t1 | MTR_3g064420 | 98.110 | 582 | 10 | 1 | 1 | 581 | 1 | 582 | 0.0 | 1189 |
| MS.gene008350.t1 | MTR_8g074040 | 50.631 | 555 | 258 | 4 | 1 | 553 | 43 | 583 | 0.0 | 602 |
| MS.gene008350.t1 | MTR_5g076240 | 53.286 | 563 | 233 | 8 | 1 | 551 | 1 | 545 | 0.0 | 590 |
| MS.gene008350.t1 | MTR_5g008800 | 50.271 | 553 | 261 | 3 | 1 | 553 | 1 | 539 | 0.0 | 587 |
| MS.gene008350.t1 | MTR_4g121600 | 51.971 | 558 | 253 | 8 | 1 | 551 | 7 | 556 | 0.0 | 578 |
| MS.gene008350.t1 | MTR_2g044180 | 51.254 | 558 | 257 | 7 | 1 | 551 | 7 | 556 | 0.0 | 575 |
| MS.gene008350.t1 | MTR_5g076220 | 51.964 | 560 | 250 | 8 | 1 | 553 | 1 | 548 | 0.0 | 555 |
| MS.gene008350.t1 | MTR_5g008820 | 47.473 | 554 | 269 | 5 | 1 | 551 | 1 | 535 | 0.0 | 552 |
| MS.gene008350.t1 | MTR_5g076200 | 50.538 | 558 | 257 | 7 | 1 | 551 | 1 | 546 | 0.0 | 542 |
| MS.gene008350.t1 | MTR_0161s0050 | 49.113 | 564 | 258 | 6 | 1 | 551 | 1 | 548 | 0.0 | 531 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008350.t1 | AT1G65880 | 61.754 | 570 | 201 | 5 | 1 | 560 | 1 | 563 | 0.0 | 715 |
| MS.gene008350.t1 | AT1G65890 | 60.900 | 578 | 213 | 5 | 1 | 570 | 1 | 573 | 0.0 | 714 |
| MS.gene008350.t1 | AT1G66120 | 58.864 | 581 | 224 | 4 | 1 | 575 | 1 | 572 | 0.0 | 703 |
| MS.gene008350.t1 | AT1G68270 | 53.509 | 570 | 217 | 7 | 1 | 560 | 1 | 532 | 0.0 | 584 |
| MS.gene008350.t1 | AT1G20560 | 50.265 | 567 | 264 | 4 | 1 | 563 | 3 | 555 | 0.0 | 581 |
| MS.gene008350.t1 | AT3G16910 | 52.330 | 558 | 252 | 8 | 1 | 551 | 9 | 559 | 0.0 | 567 |
| MS.gene008350.t1 | AT5G16340 | 49.104 | 558 | 261 | 6 | 1 | 551 | 1 | 542 | 0.0 | 562 |
| MS.gene008350.t1 | AT5G16370 | 48.566 | 558 | 264 | 5 | 1 | 551 | 1 | 542 | 0.0 | 555 |
| MS.gene008350.t1 | AT1G21530 | 50.000 | 550 | 260 | 7 | 9 | 553 | 9 | 548 | 0.0 | 550 |
| MS.gene008350.t1 | AT2G17650 | 49.107 | 560 | 265 | 4 | 1 | 550 | 48 | 597 | 0.0 | 550 |
Find 134 sgRNAs with CRISPR-Local
Find 169 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATAATGTTGAAAATAAT+GGG | 0.371892 | 3.1:+53815290 | None:intergenic |
| AAAGAGAGTCATAAAATCTC+CGG | 0.548039 | 3.1:-53815249 | MS.gene008350:CDS |
| AAAGATGTGATTATTTCAGG+AGG | 0.649768 | 3.1:-53814807 | MS.gene008350:CDS |
| AAATGCATCTCATACATTGC+TGG | 0.433221 | 3.1:+53815914 | None:intergenic |
| AACCGGTGGAGCACCACCCC+CGG | 0.538649 | 3.1:-53815216 | MS.gene008350:CDS |
| AAGAGATGGGAAAACAATGG+GGG | 0.600740 | 3.1:-53814976 | MS.gene008350:CDS |
| AAGGGAAGTGGAATCATGAT+GGG | 0.506638 | 3.1:-53814942 | MS.gene008350:CDS |
| AAGTGAAACGGGTTCCTTCA+TGG | 0.397922 | 3.1:+53816414 | None:intergenic |
| AAGTTTGTGACCAAGTGAAA+CGG | 0.494196 | 3.1:+53816402 | None:intergenic |
| AATAAAACAGGTATCAGTTT+TGG | 0.306205 | 3.1:-53815948 | MS.gene008350:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGAGAGTCATAAAATCTC+CGG | - | chr3.1:53815679-53815698 | MS.gene008350:CDS | 30.0% | |
| AAAGATGTGATTATTTCAGG+AGG | - | chr3.1:53816121-53816140 | MS.gene008350:intron | 30.0% | |
| ATCTTACCTGTTAAAGTCTT+TGG | + | chr3.1:53816394-53816413 | None:intergenic | 30.0% | |
| ATTCGTATCAAAAGCAAAAG+AGG | - | chr3.1:53815151-53815170 | MS.gene008350:CDS | 30.0% | |
| ATTTCTTCAGGAAGGTAATT+TGG | + | chr3.1:53815320-53815339 | None:intergenic | 30.0% | |
| CATTCATCTTGAATTTCTTC+AGG | + | chr3.1:53815332-53815351 | None:intergenic | 30.0% | |
| CTGAAGAAATTCAAGATGAA+TGG | - | chr3.1:53815330-53815349 | MS.gene008350:CDS | 30.0% | |
| GAAGAATTTACCACACTTTA+TGG | - | chr3.1:53816336-53816355 | MS.gene008350:intron | 30.0% | |
| GATTTACCAAAGACTTTAAC+AGG | - | chr3.1:53816385-53816404 | MS.gene008350:CDS | 30.0% | |
| TCCATAAAAACAACCATCTT+AGG | + | chr3.1:53816364-53816383 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 53814413 | 53816537 | 53814413 | ID=MS.gene008350 |
| chr3.1 | mRNA | 53814413 | 53816537 | 53814413 | ID=MS.gene008350.t1;Parent=MS.gene008350 |
| chr3.1 | exon | 53816340 | 53816537 | 53816340 | ID=MS.gene008350.t1.exon1;Parent=MS.gene008350.t1 |
| chr3.1 | CDS | 53816340 | 53816537 | 53816340 | ID=cds.MS.gene008350.t1;Parent=MS.gene008350.t1 |
| chr3.1 | exon | 53814413 | 53815960 | 53814413 | ID=MS.gene008350.t1.exon2;Parent=MS.gene008350.t1 |
| chr3.1 | CDS | 53814413 | 53815960 | 53814413 | ID=cds.MS.gene008350.t1;Parent=MS.gene008350.t1 |
| Gene Sequence |
>MS.gene008350 ATGGATAACCTACCAAAATGTCGAGCAAACTATACCATACTCACTCCTTTAACTTTCTTGATGAGAGCTTCTGCATCTTATGCAAACCGAACCTCAGTCATCCATGAAGGAACCCGTTTCACTTGGTCACAAACTTATGACCGGTGTCGCCGCCTCGCTTCCTCCCTCCGTGCTCTCAATATTGCAAAGAATGATGTTGTAAGTTCGTCACTAAATCGTGTTTTTCAAGTAGTACACAAACATCAAAGTATATAAGTATTCAAATCTCAATAAAAATTCATGCATTCATATATCTTGTATTTGATTGCAATTTTTTTGCATTATCGAGTATTGTGACTTAAACGAGATTGCAATTTAAAACGTTACATGTTCATTTCCTAGATCAGCGACCGCAATCTAGGATATGATATCAAGTAAAATTGTATTGCCAATATTTGATACTGCATTTTGTCCGCAATATCAAATATTGTAATAATACACTAACTCAACGTGACTACAATTCAAAATGTTACATACTATTATGATTAAGATTCGCGCGATTTATAAAAACTAAATTGGTTTTTGTTTAATAAAACAGGTATCAGTTTTGGCACCAAACATTCCAGCAATGTATGAGATGCATTTTGCAGTACCTATGGCCGGTGCTGTCCTAAACACCATCAACCACCGTCTCAATGCGGCCAACATAGCCACCATTCTTCGACACTCTGAGGCGAAAGTCTTCTTCGTAGACTACGAATTCGTATCAAAAGCAAAAGAGGCCTTAAGATTACTCATGGAGGAAAAAGATCAAAAAGAACAATACTCATCACTCCCACTAGTGATTGTGATAGATGACATAAACACTCCCACCGGAATCCGGCTAGGCGAGCTCGAATACGAGCAAATGGTTCATCATGGTAATCCAAATTACCTTCCTGAAGAAATTCAAGATGAATGGTCACCAATTACATTGAATTACACATCAGGTACAACTTCAGAACCAAAAGGTGTTGTTTATAGTCATAGAGGAGCTTATTTAAGTACTTTGAGTTTAATCCTTGGTTGGGAAATGGGTAGTGAACCAGTTTATCTATGGACATTACCTATGTTCCATTGTAATGGTTGGACATTCACTTGGGGTGTAGCTGCAAGAGGCGGTACTAATATTTGTATCAGAAACACTGCAGCATCTGATATATATAGAGCCATAAATCTCTATAATGTGACACACATGTGTTGCGCACCCATTATTTTCAACATTATTTTAGGCGCGAAACCAAGCGAAAAGAGAGTCATAAAATCTCCGGTGAATATACTAACCGGTGGAGCACCACCCCCGGCGTCCCTACTTGAGAAAATCGAGCCACTTGGTTTTTATGTAACACATGCTTATGGTCTGACTGAGGCAACAGGGCCAGCACTTGTTTGTGAGTGGCAGAAGAAATGGAATGTGTTGCCGAAAAGAGAGCAATCGATGCTGAAAGCGCGACAAGGGGTTAGTGTTTTGACACTTGCTGATGTTGATGTGAAGAATCTTGAAACAATGGAGAGTGTGGTAAGAGATGGGAAAACAATGGGGGAGATTATGTTGAAGGGAAGTGGAATCATGATGGGGTATTTTAAAGATAAAGAAGCGACGGAGAAAGCTTTTGGGGATGGTTGGTTTAGAACTGGTGATGTTGGTGTTATACATAAAGATGGTTATTTGGAAATTAAAGATAGGTCTAAAGATGTGATTATTTCAGGAGGTGAAAATATTAGTAGTGTTGAAGTTGAAAATGTTCTTTATAGTCATCCTAAGGTTTTAGAAGCTGCTGTGGTTGCAATGCCACATCCAAAATGGGGTGAAAGTCCTTGTGCTTTTGTGACACTAAGGAAGAATGAGGAGGAAAAAAGTGATTCTTGTTGTGTTACTGAGGATGAGATAATTACCTATTGTAGGAAGAATTTACCACACTTTATGGTACCTAAGATGGTTGTTTTTATGGAGGATTTACCAAAGACTTTAACAGGTAAGATTCAGAAATTTGAATTAAGAGCCAAAGCTAAGTGTTTTGTGGTAAATGATGAGAAAAATAAGAAGAAGCCTAATAATCAAGTCAATCATAATAATGACCAGATTATGGCTCTATCTCGTCTTTGA |
| Protein sequence |
>MS.gene008350.t1 MDNLPKCRANYTILTPLTFLMRASASYANRTSVIHEGTRFTWSQTYDRCRRLASSLRALNIAKNDVVSVLAPNIPAMYEMHFAVPMAGAVLNTINHRLNAANIATILRHSEAKVFFVDYEFVSKAKEALRLLMEEKDQKEQYSSLPLVIVIDDINTPTGIRLGELEYEQMVHHGNPNYLPEEIQDEWSPITLNYTSGTTSEPKGVVYSHRGAYLSTLSLILGWEMGSEPVYLWTLPMFHCNGWTFTWGVAARGGTNICIRNTAASDIYRAINLYNVTHMCCAPIIFNIILGAKPSEKRVIKSPVNILTGGAPPPASLLEKIEPLGFYVTHAYGLTEATGPALVCEWQKKWNVLPKREQSMLKARQGVSVLTLADVDVKNLETMESVVRDGKTMGEIMLKGSGIMMGYFKDKEATEKAFGDGWFRTGDVGVIHKDGYLEIKDRSKDVIISGGENISSVEVENVLYSHPKVLEAAVVAMPHPKWGESPCAFVTLRKNEEEKSDSCCVTEDEIITYCRKNLPHFMVPKMVVFMEDLPKTLTGKIQKFELRAKAKCFVVNDEKNKKKPNNQVNHNNDQIMALSRL |
