Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32049.t1 | AAF19528.1 | 98.4 | 243 | 4 | 0 | 1 | 243 | 1 | 243 | 1.40E-126 | 463.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32049.t1 | Q9LE82 | 67.6 | 244 | 72 | 3 | 1 | 243 | 1 | 238 | 1.2e-84 | 315.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32049.t1 | Q9SBS1 | 98.4 | 243 | 4 | 0 | 1 | 243 | 1 | 243 | 1.0e-126 | 463.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049411 | MS.gene32049 | 0.806277 | 8.90E-50 | -1.69E-46 |
| MS.gene050838 | MS.gene32049 | 0.867253 | 1.57E-65 | -1.69E-46 |
| MS.gene050945 | MS.gene32049 | 0.819104 | 1.38E-52 | -1.69E-46 |
| MS.gene051003 | MS.gene32049 | 0.818681 | 1.73E-52 | -1.69E-46 |
| MS.gene051233 | MS.gene32049 | 0.800065 | 1.72E-48 | -1.69E-46 |
| MS.gene051234 | MS.gene32049 | 0.841048 | 6.02E-58 | -1.69E-46 |
| MS.gene051343 | MS.gene32049 | 0.806074 | 9.81E-50 | -1.69E-46 |
| MS.gene051642 | MS.gene32049 | 0.806132 | 9.54E-50 | -1.69E-46 |
| MS.gene051676 | MS.gene32049 | 0.820969 | 5.18E-53 | -1.69E-46 |
| MS.gene05188 | MS.gene32049 | 0.808237 | 3.42E-50 | -1.69E-46 |
| MS.gene051902 | MS.gene32049 | 0.820607 | 6.27E-53 | -1.69E-46 |
| MS.gene051975 | MS.gene32049 | 0.821861 | 3.22E-53 | -1.69E-46 |
| MS.gene052072 | MS.gene32049 | 0.813628 | 2.32E-51 | -1.69E-46 |
| MS.gene052296 | MS.gene32049 | 0.827507 | 1.51E-54 | -1.69E-46 |
| MS.gene052947 | MS.gene32049 | 0.818714 | 1.70E-52 | -1.69E-46 |
| MS.gene053058 | MS.gene32049 | 0.810552 | 1.09E-50 | -1.69E-46 |
| MS.gene053564 | MS.gene32049 | 0.832574 | 8.75E-56 | -1.69E-46 |
| MS.gene053786 | MS.gene32049 | 0.805514 | 1.29E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene32049 | 0.80507 | 1.59E-49 | -1.69E-46 |
| MS.gene055765 | MS.gene32049 | 0.801933 | 7.13E-49 | -1.69E-46 |
| MS.gene056215 | MS.gene32049 | 0.809612 | 1.74E-50 | -1.69E-46 |
| MS.gene05689 | MS.gene32049 | 0.808477 | 3.04E-50 | -1.69E-46 |
| MS.gene056986 | MS.gene32049 | 0.807636 | 4.59E-50 | -1.69E-46 |
| MS.gene057094 | MS.gene32049 | 0.814721 | 1.33E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene32049 | 0.819403 | 1.18E-52 | -1.69E-46 |
| MS.gene057694 | MS.gene32049 | 0.800068 | 1.71E-48 | -1.69E-46 |
| MS.gene058074 | MS.gene32049 | 0.824231 | 9.03E-54 | -1.69E-46 |
| MS.gene059146 | MS.gene32049 | 0.834262 | 3.32E-56 | -1.69E-46 |
| MS.gene059171 | MS.gene32049 | 0.808398 | 3.16E-50 | -1.69E-46 |
| MS.gene059227 | MS.gene32049 | 0.83553 | 1.59E-56 | -1.69E-46 |
| MS.gene059778 | MS.gene32049 | 0.800753 | 1.24E-48 | -1.69E-46 |
| MS.gene06011 | MS.gene32049 | 0.806675 | 7.33E-50 | -1.69E-46 |
| MS.gene060343 | MS.gene32049 | -0.802317 | 5.94E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene32049 | 0.816628 | 5.01E-52 | -1.69E-46 |
| MS.gene061100 | MS.gene32049 | 0.853479 | 2.33E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene32049 | MS.gene015341 | PPI |
| MS.gene32049 | MS.gene21497 | PPI |
| MS.gene32049 | MS.gene32605 | PPI |
| MS.gene54215 | MS.gene32049 | PPI |
| MS.gene32049 | MS.gene014809 | PPI |
| MS.gene33355 | MS.gene32049 | PPI |
| MS.gene32049 | MS.gene32604 | PPI |
| MS.gene32049 | MS.gene32606 | PPI |
| MS.gene32049 | MS.gene90308 | PPI |
| MS.gene32049 | MS.gene91712 | PPI |
| MS.gene32049 | MS.gene014807 | PPI |
| MS.gene32049 | MS.gene072925 | PPI |
| MS.gene32049 | MS.gene64546 | PPI |
| MS.gene32049 | MS.gene79541 | PPI |
| MS.gene32049 | MS.gene072924 | PPI |
| MS.gene32049 | MS.gene014808 | PPI |
| MS.gene32049 | MS.gene072926 | PPI |
| MS.gene32049 | MS.gene24901 | PPI |
| MS.gene32049 | MS.gene09683 | PPI |
| MS.gene32049 | MS.gene033985 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32049.t1 | MTR_1g072250 | 96.248 | 533 | 20 | 0 | 1 | 533 | 1 | 533 | 0.0 | 1035 |
| MS.gene32049.t1 | MTR_1g105040 | 68.880 | 527 | 162 | 2 | 9 | 533 | 6 | 532 | 0.0 | 712 |
| MS.gene32049.t1 | MTR_5g035590 | 27.742 | 310 | 193 | 5 | 204 | 483 | 39 | 347 | 1.45e-22 | 100 |
| MS.gene32049.t1 | MTR_5g035590 | 27.138 | 269 | 196 | 0 | 224 | 492 | 32 | 300 | 3.13e-20 | 93.6 |
| MS.gene32049.t1 | MTR_5g035590 | 24.658 | 292 | 158 | 7 | 204 | 462 | 151 | 413 | 1.46e-11 | 67.0 |
| MS.gene32049.t1 | MTR_5g035590 | 26.774 | 310 | 196 | 4 | 204 | 483 | 216 | 524 | 3.44e-22 | 100 |
| MS.gene32049.t1 | MTR_5g035590 | 27.138 | 269 | 196 | 0 | 224 | 492 | 209 | 477 | 5.87e-20 | 94.0 |
| MS.gene32049.t1 | MTR_5g035590 | 24.658 | 292 | 158 | 7 | 204 | 462 | 328 | 590 | 1.72e-11 | 67.0 |
| MS.gene32049.t1 | MTR_3g102500 | 46.067 | 89 | 45 | 2 | 17 | 104 | 36 | 122 | 9.20e-18 | 81.3 |
| MS.gene32049.t1 | MTR_1g018710 | 35.000 | 100 | 63 | 1 | 6 | 105 | 17 | 114 | 2.64e-14 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32049.t1 | AT3G63130 | 69.109 | 505 | 149 | 3 | 1 | 504 | 1 | 499 | 0.0 | 706 |
| MS.gene32049.t1 | AT3G63130 | 69.109 | 505 | 149 | 3 | 1 | 504 | 1 | 499 | 0.0 | 706 |
| MS.gene32049.t1 | AT5G19320 | 67.546 | 493 | 158 | 2 | 11 | 501 | 10 | 502 | 0.0 | 637 |
| MS.gene32049.t1 | AT5G19320 | 67.546 | 493 | 158 | 2 | 11 | 501 | 10 | 502 | 0.0 | 637 |
| MS.gene32049.t1 | AT3G06000 | 50.000 | 184 | 79 | 2 | 321 | 504 | 16 | 186 | 2.34e-47 | 164 |
| MS.gene32049.t1 | AT1G10510 | 30.292 | 274 | 191 | 0 | 219 | 492 | 203 | 476 | 3.51e-25 | 110 |
| MS.gene32049.t1 | AT1G10510 | 30.249 | 281 | 191 | 4 | 211 | 487 | 307 | 586 | 5.64e-21 | 97.1 |
| MS.gene32049.t1 | AT1G47200 | 36.697 | 109 | 67 | 1 | 13 | 121 | 56 | 162 | 6.20e-15 | 73.6 |
| MS.gene32049.t1 | AT5G43070 | 41.111 | 90 | 51 | 1 | 12 | 101 | 39 | 126 | 7.48e-15 | 72.8 |
Find 116 sgRNAs with CRISPR-Local
Find 139 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCACCGTCACTGGAAATTT+TGG | 0.158218 | 1.2:+55208292 | MS.gene32049:CDS |
| GCAACTTAAATCCAGGTTTC+TGG | 0.190819 | 1.2:-55208541 | None:intergenic |
| CCTCAGTTATTTGAACTTAG+AGG | 0.267220 | 1.2:+55208229 | MS.gene32049:CDS |
| CGTCTCCAGTCATGTTATTA+TGG | 0.271627 | 1.2:-55207980 | None:intergenic |
| ACCTGACATGCTGGGTCCTT+TGG | 0.274094 | 1.2:+55208631 | MS.gene32049:CDS |
| GGTCGCAGAGCTTTCATTAA+TGG | 0.274791 | 1.2:+55207540 | MS.gene32049:CDS |
| CATATCTTTGTAAAAGAGTT+TGG | 0.287321 | 1.2:-55207600 | None:intergenic |
| GTCGCAGAGCTTTCATTAAT+GGG | 0.308702 | 1.2:+55207541 | MS.gene32049:CDS |
| CAAGAGGGAAGGAGGAGAAT+GGG | 0.312236 | 1.2:+55207460 | MS.gene32049:CDS |
| TTGCTCTTGCTGAAGCGCTT+GGG | 0.313724 | 1.2:+55208096 | MS.gene32049:CDS |
| TGTTTCAGTAATAGAAGTTT+TGG | 0.325309 | 1.2:+55207621 | MS.gene32049:CDS |
| TTCATCTTTCAATTCATTCT+CGG | 0.330579 | 1.2:-55208405 | None:intergenic |
| ACTTCAATAGCTTCTGCTTC+TGG | 0.333568 | 1.2:-55207735 | None:intergenic |
| GTTGCTCTTGCTGAAGCGCT+TGG | 0.338191 | 1.2:+55208095 | MS.gene32049:CDS |
| CCATTCTCCTCCTTCCCTCT+TGG | 0.345911 | 1.2:-55207459 | None:intergenic |
| TCATCAACGTCCTCTCCTTC+AGG | 0.355513 | 1.2:-55208665 | None:intergenic |
| TGTGAAACGTTCCCCAGCTT+TGG | 0.391512 | 1.2:+55208028 | MS.gene32049:CDS |
| AGCCCTTGCTAATGCTTTGA+AGG | 0.393810 | 1.2:+55208265 | MS.gene32049:CDS |
| CCCTTCCACTGAGAAGCTTA+AGG | 0.398979 | 1.2:+55207947 | MS.gene32049:CDS |
| CCAAGAGGGAAGGAGGAGAA+TGG | 0.407320 | 1.2:+55207459 | MS.gene32049:CDS |
| AGAGCTTCTAAAACCATTGA+TGG | 0.410753 | 1.2:+55207575 | MS.gene32049:CDS |
| GAAGGAATCTGCACCGTCAC+TGG | 0.422448 | 1.2:+55208283 | MS.gene32049:CDS |
| AAAGAGTTTGGTCCCATCAA+TGG | 0.422926 | 1.2:-55207588 | None:intergenic |
| CACCCATGGCATTATTTGAC+AGG | 0.423148 | 1.2:-55207809 | None:intergenic |
| TCCAAAGGACCCAGCATGTC+AGG | 0.424980 | 1.2:-55208632 | None:intergenic |
| GAGCTTTATTTGATGAATGA+TGG | 0.428296 | 1.2:+55207885 | MS.gene32049:CDS |
| ACAAATTTGATCACATGGTC+TGG | 0.437019 | 1.2:+55208491 | MS.gene32049:CDS |
| TGGGGAGTTGATATCTGAAA+AGG | 0.439605 | 1.2:+55207479 | MS.gene32049:CDS |
| AAATAATGCCATGGGTGAAA+AGG | 0.442002 | 1.2:+55207815 | MS.gene32049:CDS |
| GCCAACTTCATTTCTGATGA+AGG | 0.446090 | 1.2:+55208575 | MS.gene32049:CDS |
| CACCGAAAATCTTCCAAAGC+TGG | 0.450378 | 1.2:-55208041 | None:intergenic |
| AATAATGCCATGGGTGAAAA+GGG | 0.461364 | 1.2:+55207816 | MS.gene32049:CDS |
| CATAAGCTTACTCGATTCCT+TGG | 0.462204 | 1.2:-55207415 | None:intergenic |
| AGAAAATAGACGGAGTCGTC+AGG | 0.468919 | 1.2:-55207254 | None:intergenic |
| CGGTGTTCATCTACCAGAGT+AGG | 0.471423 | 1.2:+55208059 | MS.gene32049:CDS |
| AGGTGCAGGTTTAATTAGTA+AGG | 0.472052 | 1.2:+55208427 | MS.gene32049:CDS |
| ATCTTTAATTGATATTAGCA+TGG | 0.476224 | 1.2:-55207670 | None:intergenic |
| CCTTAAATCACAAAATGACT+TGG | 0.476235 | 1.2:+55207860 | MS.gene32049:CDS |
| GGCCTCCAAGTCAGAGTACT+AGG | 0.483558 | 1.2:+55207202 | MS.gene32049:CDS |
| ATCGAGTAAGCTTATGTTGG+AGG | 0.487701 | 1.2:+55207422 | MS.gene32049:CDS |
| ACCGAAAATCTTCCAAAGCT+GGG | 0.488948 | 1.2:-55208040 | None:intergenic |
| GAAGCTGTTGTCCAGAAACC+TGG | 0.491319 | 1.2:+55208530 | MS.gene32049:CDS |
| CGGATAAATTTAACTTGGTG+AGG | 0.494899 | 1.2:-55208385 | None:intergenic |
| TGCTCTTGCTGAAGCGCTTG+GGG | 0.498032 | 1.2:+55208097 | MS.gene32049:CDS |
| TAAATCACAAAATGACTTGG+AGG | 0.498668 | 1.2:+55207863 | MS.gene32049:CDS |
| GAGAATGAATTGAAAGATGA+AGG | 0.503082 | 1.2:+55208407 | MS.gene32049:CDS |
| CATTTCCATAATAACATGAC+TGG | 0.503127 | 1.2:+55207975 | MS.gene32049:CDS |
| AACATGTTTGGTGTGGAAGC+TGG | 0.506518 | 1.2:+55208155 | MS.gene32049:CDS |
| CCAACTTCATTTCTGATGAA+GGG | 0.507173 | 1.2:+55208576 | MS.gene32049:CDS |
| GATTTGCGTGACAACATGTT+TGG | 0.507634 | 1.2:+55208143 | MS.gene32049:CDS |
| AATTAAAGATCAACTGAAAG+AGG | 0.511896 | 1.2:+55207683 | MS.gene32049:CDS |
| TCTGTAAGATCAGCAAATAC+AGG | 0.513065 | 1.2:-55208200 | None:intergenic |
| ATATTCAGCAACTTAAATCC+AGG | 0.517009 | 1.2:-55208548 | None:intergenic |
| GCAGAACTCCCATCACCATC+AGG | 0.519389 | 1.2:-55207378 | None:intergenic |
| TGCGACCCAACACTTTGAAA+AGG | 0.522092 | 1.2:+55207353 | MS.gene32049:CDS |
| TGCTGTGCAAATTTATGCCA+AGG | 0.523338 | 1.2:+55207398 | MS.gene32049:CDS |
| ACCTTAAGCTTCTCAGTGGA+AGG | 0.523910 | 1.2:-55207948 | None:intergenic |
| GGGGAGTTGATATCTGAAAA+GGG | 0.524553 | 1.2:+55207480 | MS.gene32049:CDS |
| TCTTTAATTGATATTAGCAT+GGG | 0.527745 | 1.2:-55207669 | None:intergenic |
| AACCTAGTACTCTGACTTGG+AGG | 0.528886 | 1.2:-55207204 | None:intergenic |
| ATGAATCTTCTTAAATCTCA+AGG | 0.529266 | 1.2:-55208739 | None:intergenic |
| GAAAAGGAGCCTGATGGTGA+TGG | 0.530765 | 1.2:+55207369 | MS.gene32049:CDS |
| TAACAAAGATGAGGCTGAGA+AGG | 0.532357 | 1.2:+55207296 | MS.gene32049:CDS |
| AACAGCTTCTGCCAACAGCT+TGG | 0.534878 | 1.2:-55208516 | None:intergenic |
| TGTTAGTTGAACGAATGGTC+AGG | 0.535510 | 1.2:+55207229 | MS.gene32049:CDS |
| TAACCTGTCAAATAATGCCA+TGG | 0.536436 | 1.2:+55207806 | MS.gene32049:CDS |
| ATTCTCGGATAAATTTAACT+TGG | 0.537197 | 1.2:-55208390 | None:intergenic |
| CCCTTCATCAGAAATGAAGT+TGG | 0.537535 | 1.2:-55208576 | None:intergenic |
| TTTGTGATTTAAGGAGTGCC+CGG | 0.539029 | 1.2:-55207851 | None:intergenic |
| AGGTTTAATTAGTAAGGCGC+TGG | 0.544570 | 1.2:+55208433 | MS.gene32049:CDS |
| AAGAGGGAAGGAGGAGAATG+GGG | 0.549859 | 1.2:+55207461 | MS.gene32049:CDS |
| GTTGATGTTAGTTGAACGAA+TGG | 0.555548 | 1.2:+55207224 | MS.gene32049:CDS |
| TCTACCAGAGTAGGCTCTGA+AGG | 0.560072 | 1.2:+55208068 | MS.gene32049:CDS |
| GAAGGATGCAAAGGAAATTG+AGG | 0.561870 | 1.2:+55207314 | MS.gene32049:CDS |
| AAGAGGACCAAGAGGGAAGG+AGG | 0.565309 | 1.2:+55207452 | MS.gene32049:CDS |
| ATCAACCTAGTACTCTGACT+TGG | 0.566163 | 1.2:-55207207 | None:intergenic |
| ATTCCTTCAAAGCATTAGCA+AGG | 0.570197 | 1.2:-55208268 | None:intergenic |
| AGGAGAGGACGTTGATGAAG+AGG | 0.572691 | 1.2:+55208670 | MS.gene32049:CDS |
| AGTAAGGCGCTGGAAGGTCG+TGG | 0.574225 | 1.2:+55208443 | MS.gene32049:CDS |
| TTCCTTCAAAGCATTAGCAA+GGG | 0.575261 | 1.2:-55208267 | None:intergenic |
| GAGCTTCTAAAACCATTGAT+GGG | 0.582760 | 1.2:+55207576 | MS.gene32049:CDS |
| GAACACTTTCAATCAAGTTG+TGG | 0.586485 | 1.2:+55207181 | MS.gene32049:CDS |
| AACCTGTCAAATAATGCCAT+GGG | 0.587490 | 1.2:+55207807 | MS.gene32049:CDS |
| TCAGGATCATTCTCATCCAA+AGG | 0.589310 | 1.2:-55208647 | None:intergenic |
| GGAATCGAGTAAGCTTATGT+TGG | 0.591102 | 1.2:+55207419 | MS.gene32049:CDS |
| GACTTGGAGTCAAAACTGAA+AGG | 0.592400 | 1.2:+55208716 | MS.gene32049:CDS |
| ACCACCTTCAGAGCCTACTC+TGG | 0.593405 | 1.2:-55208072 | None:intergenic |
| ATGGTCTGGAGCCAAGCTGT+TGG | 0.593553 | 1.2:+55208505 | MS.gene32049:CDS |
| TGATTCTGATAATGATGACT+TGG | 0.594422 | 1.2:+55208700 | MS.gene32049:CDS |
| CAGAGCTTTCATTAATGGGG+AGG | 0.598559 | 1.2:+55207545 | MS.gene32049:CDS |
| TTAATTAGTAAGGCGCTGGA+AGG | 0.600794 | 1.2:+55208437 | MS.gene32049:CDS |
| GAATTGAAAGATGAAGGTGC+AGG | 0.601464 | 1.2:+55208413 | MS.gene32049:CDS |
| TAATGCCATGGGTGAAAAGG+GGG | 0.611795 | 1.2:+55207818 | MS.gene32049:CDS |
| AAAAGGAGCCTGATGGTGAT+GGG | 0.618474 | 1.2:+55207370 | MS.gene32049:CDS |
| TACTTTCTAGAGAAAATAGA+CGG | 0.618656 | 1.2:-55207264 | None:intergenic |
| GATGAATGATGGTATATCAG+AGG | 0.619244 | 1.2:+55207896 | MS.gene32049:CDS |
| TGAGGCTGAGAAGGATGCAA+AGG | 0.619486 | 1.2:+55207305 | MS.gene32049:CDS |
| ATATCCAAAATTTCCAGTGA+CGG | 0.620331 | 1.2:-55208296 | None:intergenic |
| GAAAAGAGGACCAAGAGGGA+AGG | 0.620402 | 1.2:+55207449 | MS.gene32049:CDS |
| CACTTTGAAAAGGAGCCTGA+TGG | 0.620964 | 1.2:+55207363 | MS.gene32049:CDS |
| CCTTAAGCTTCTCAGTGGAA+GGG | 0.628373 | 1.2:-55207947 | None:intergenic |
| TGAGTACAAATTTGATCACA+TGG | 0.630339 | 1.2:+55208486 | MS.gene32049:CDS |
| CCGAAAATCTTCCAAAGCTG+GGG | 0.630768 | 1.2:-55208039 | None:intergenic |
| TTGGATGAGAATGATCCTGA+AGG | 0.631234 | 1.2:+55208650 | MS.gene32049:CDS |
| ATAATGCCATGGGTGAAAAG+GGG | 0.631439 | 1.2:+55207817 | MS.gene32049:CDS |
| TGAGAATGATCCTGAAGGAG+AGG | 0.636079 | 1.2:+55208655 | MS.gene32049:CDS |
| TGATTGAAAGTGTTCGATGT+TGG | 0.637029 | 1.2:-55207173 | None:intergenic |
| AATAACATGACTGGAGACGA+AGG | 0.637173 | 1.2:+55207984 | MS.gene32049:CDS |
| TGGACTTCTTAACAAAGATG+AGG | 0.646938 | 1.2:+55207287 | MS.gene32049:CDS |
| AAGAACCTTAAGCTTCTCAG+TGG | 0.653888 | 1.2:-55207952 | None:intergenic |
| CCTCTAAGTTCAAATAACTG+AGG | 0.660358 | 1.2:-55208229 | None:intergenic |
| GCGTGACAACATGTTTGGTG+TGG | 0.661175 | 1.2:+55208148 | MS.gene32049:CDS |
| TCGCAGAGCTTTCATTAATG+GGG | 0.661648 | 1.2:+55207542 | MS.gene32049:CDS |
| TATTTGAACTTAGAGGACGA+TGG | 0.662229 | 1.2:+55208236 | MS.gene32049:CDS |
| ACCAGAGTAGGCTCTGAAGG+TGG | 0.671940 | 1.2:+55208071 | MS.gene32049:CDS |
| AGTGTTCGATGTTGGTACGA+CGG | 0.709109 | 1.2:-55207165 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCTTTAATTGATATTAGCA+TGG | - | chr1.2:55207673-55207692 | None:intergenic | 20.0% |
| !! | TCTTTAATTGATATTAGCAT+GGG | - | chr1.2:55207672-55207691 | None:intergenic | 20.0% |
| ! | AATTAAAGATCAACTGAAAG+AGG | + | chr1.2:55207683-55207702 | MS.gene32049:CDS | 25.0% |
| ! | ATTCTCGGATAAATTTAACT+TGG | - | chr1.2:55208393-55208412 | None:intergenic | 25.0% |
| ! | CATATCTTTGTAAAAGAGTT+TGG | - | chr1.2:55207603-55207622 | None:intergenic | 25.0% |
| ! | TTCATCTTTCAATTCATTCT+CGG | - | chr1.2:55208408-55208427 | None:intergenic | 25.0% |
| !! | ATATTTTCTTCTGCATTAGA+AGG | + | chr1.2:55207765-55207784 | MS.gene32049:CDS | 25.0% |
| !! | TACTTTCTAGAGAAAATAGA+CGG | - | chr1.2:55207267-55207286 | None:intergenic | 25.0% |
| !! | TGTTTCAGTAATAGAAGTTT+TGG | + | chr1.2:55207621-55207640 | MS.gene32049:CDS | 25.0% |
| !!! | AAACTGTTTTTGACATTTCT+GGG | + | chr1.2:55207517-55207536 | MS.gene32049:CDS | 25.0% |
| !!! | TCTATTTTCTCTAGAAAGTA+TGG | + | chr1.2:55207267-55207286 | MS.gene32049:CDS | 25.0% |
| ATATCCAAAATTTCCAGTGA+CGG | - | chr1.2:55208299-55208318 | None:intergenic | 30.0% | |
| ATATTCAGCAACTTAAATCC+AGG | - | chr1.2:55208551-55208570 | None:intergenic | 30.0% | |
| CATTTCCATAATAACATGAC+TGG | + | chr1.2:55207975-55207994 | MS.gene32049:CDS | 30.0% | |
| CCTTAAATCACAAAATGACT+TGG | + | chr1.2:55207860-55207879 | MS.gene32049:CDS | 30.0% | |
| GAGAATGAATTGAAAGATGA+AGG | + | chr1.2:55208407-55208426 | MS.gene32049:CDS | 30.0% | |
| TGAGTACAAATTTGATCACA+TGG | + | chr1.2:55208486-55208505 | MS.gene32049:CDS | 30.0% | |
| ! | ATCTGTCAGATTTTATTGCT+GGG | + | chr1.2:55207709-55207728 | MS.gene32049:CDS | 30.0% |
| ! | TAAATCACAAAATGACTTGG+AGG | + | chr1.2:55207863-55207882 | MS.gene32049:CDS | 30.0% |
| ! | TGATTCTGATAATGATGACT+TGG | + | chr1.2:55208700-55208719 | MS.gene32049:CDS | 30.0% |
| !! | CCAAGTCATTTTGTGATTTA+AGG | - | chr1.2:55207863-55207882 | None:intergenic | 30.0% |
| !! | GAGCTTTATTTGATGAATGA+TGG | + | chr1.2:55207885-55207904 | MS.gene32049:CDS | 30.0% |
| !!! | AACTGTTTTTGACATTTCTG+GGG | + | chr1.2:55207518-55207537 | MS.gene32049:CDS | 30.0% |
| !!! | CAGTAATAGAAGTTTTGGTT+TGG | + | chr1.2:55207626-55207645 | MS.gene32049:CDS | 30.0% |
| !!! | GAAACTGTTTTTGACATTTC+TGG | + | chr1.2:55207516-55207535 | MS.gene32049:CDS | 30.0% |
| AAATAATGCCATGGGTGAAA+AGG | + | chr1.2:55207815-55207834 | MS.gene32049:CDS | 35.0% | |
| AACCTGTCAAATAATGCCAT+GGG | + | chr1.2:55207807-55207826 | MS.gene32049:CDS | 35.0% | |
| AATAATGCCATGGGTGAAAA+GGG | + | chr1.2:55207816-55207835 | MS.gene32049:CDS | 35.0% | |
| ACAAATTTGATCACATGGTC+TGG | + | chr1.2:55208491-55208510 | MS.gene32049:CDS | 35.0% | |
| AGGTGCAGGTTTAATTAGTA+AGG | + | chr1.2:55208427-55208446 | MS.gene32049:CDS | 35.0% | |
| CCAACTTCATTTCTGATGAA+GGG | + | chr1.2:55208576-55208595 | MS.gene32049:CDS | 35.0% | |
| CCTCAGTTATTTGAACTTAG+AGG | + | chr1.2:55208229-55208248 | MS.gene32049:CDS | 35.0% | |
| CCTCTAAGTTCAAATAACTG+AGG | - | chr1.2:55208232-55208251 | None:intergenic | 35.0% | |
| GATGAATGATGGTATATCAG+AGG | + | chr1.2:55207896-55207915 | MS.gene32049:CDS | 35.0% | |
| GTTGATGTTAGTTGAACGAA+TGG | + | chr1.2:55207224-55207243 | MS.gene32049:CDS | 35.0% | |
| TAACCTGTCAAATAATGCCA+TGG | + | chr1.2:55207806-55207825 | MS.gene32049:CDS | 35.0% | |
| TATTTGAACTTAGAGGACGA+TGG | + | chr1.2:55208236-55208255 | MS.gene32049:CDS | 35.0% | |
| TCTGTAAGATCAGCAAATAC+AGG | - | chr1.2:55208203-55208222 | None:intergenic | 35.0% | |
| TGGACTTCTTAACAAAGATG+AGG | + | chr1.2:55207287-55207306 | MS.gene32049:CDS | 35.0% | |
| ! | ATTCCTTCAAAGCATTAGCA+AGG | - | chr1.2:55208271-55208290 | None:intergenic | 35.0% |
| ! | GAACACTTTCAATCAAGTTG+TGG | + | chr1.2:55207181-55207200 | MS.gene32049:CDS | 35.0% |
| ! | GATCTGTCAGATTTTATTGC+TGG | + | chr1.2:55207708-55207727 | MS.gene32049:CDS | 35.0% |
| ! | GTCACTGGAAATTTTGGATA+TGG | + | chr1.2:55208298-55208317 | MS.gene32049:CDS | 35.0% |
| ! | TGGAAATTTTGGATATGGCT+GGG | + | chr1.2:55208303-55208322 | MS.gene32049:CDS | 35.0% |
| ! | TTCCTTCAAAGCATTAGCAA+GGG | - | chr1.2:55208270-55208289 | None:intergenic | 35.0% |
| !! | AGAGCTTCTAAAACCATTGA+TGG | + | chr1.2:55207575-55207594 | MS.gene32049:CDS | 35.0% |
| !! | CGGATAAATTTAACTTGGTG+AGG | - | chr1.2:55208388-55208407 | None:intergenic | 35.0% |
| !! | GAGCTTCTAAAACCATTGAT+GGG | + | chr1.2:55207576-55207595 | MS.gene32049:CDS | 35.0% |
| !! | TGATTGAAAGTGTTCGATGT+TGG | - | chr1.2:55207176-55207195 | None:intergenic | 35.0% |
| !! | TTTTGAAAAGAGGACCAAGA+GGG | + | chr1.2:55207445-55207464 | MS.gene32049:CDS | 35.0% |
| !!! | ACTGTTTTTGACATTTCTGG+GGG | + | chr1.2:55207519-55207538 | MS.gene32049:CDS | 35.0% |
| !!! | ATGTTGGAGGTTTTGAAAAG+AGG | + | chr1.2:55207435-55207454 | MS.gene32049:CDS | 35.0% |
| AAAAACTCACCTGACATGCT+GGG | + | chr1.2:55208623-55208642 | MS.gene32049:CDS | 40.0% | |
| AAAGAGTTTGGTCCCATCAA+TGG | - | chr1.2:55207591-55207610 | None:intergenic | 40.0% | |
| AAGAACCTTAAGCTTCTCAG+TGG | - | chr1.2:55207955-55207974 | None:intergenic | 40.0% | |
| AATAACATGACTGGAGACGA+AGG | + | chr1.2:55207984-55208003 | MS.gene32049:CDS | 40.0% | |
| ACCGAAAATCTTCCAAAGCT+GGG | - | chr1.2:55208043-55208062 | None:intergenic | 40.0% | |
| ACTTCAATAGCTTCTGCTTC+TGG | - | chr1.2:55207738-55207757 | None:intergenic | 40.0% | |
| AGGTTTAATTAGTAAGGCGC+TGG | + | chr1.2:55208433-55208452 | MS.gene32049:CDS | 40.0% | |
| ATAATGCCATGGGTGAAAAG+GGG | + | chr1.2:55207817-55207836 | MS.gene32049:CDS | 40.0% | |
| ATCAACCTAGTACTCTGACT+TGG | - | chr1.2:55207210-55207229 | None:intergenic | 40.0% | |
| ATCGAGTAAGCTTATGTTGG+AGG | + | chr1.2:55207422-55207441 | MS.gene32049:CDS | 40.0% | |
| CATAAGCTTACTCGATTCCT+TGG | - | chr1.2:55207418-55207437 | None:intergenic | 40.0% | |
| CCCTTCATCAGAAATGAAGT+TGG | - | chr1.2:55208579-55208598 | None:intergenic | 40.0% | |
| CGTCTCCAGTCATGTTATTA+TGG | - | chr1.2:55207983-55208002 | None:intergenic | 40.0% | |
| GAAGGATGCAAAGGAAATTG+AGG | + | chr1.2:55207314-55207333 | MS.gene32049:CDS | 40.0% | |
| GACTTGGAGTCAAAACTGAA+AGG | + | chr1.2:55208716-55208735 | MS.gene32049:CDS | 40.0% | |
| GATTTGCGTGACAACATGTT+TGG | + | chr1.2:55208143-55208162 | MS.gene32049:CDS | 40.0% | |
| GCAACTTAAATCCAGGTTTC+TGG | - | chr1.2:55208544-55208563 | None:intergenic | 40.0% | |
| GCCAACTTCATTTCTGATGA+AGG | + | chr1.2:55208575-55208594 | MS.gene32049:CDS | 40.0% | |
| GGAATCGAGTAAGCTTATGT+TGG | + | chr1.2:55207419-55207438 | MS.gene32049:CDS | 40.0% | |
| GTCGCAGAGCTTTCATTAAT+GGG | + | chr1.2:55207541-55207560 | MS.gene32049:CDS | 40.0% | |
| TAAAAACTCACCTGACATGC+TGG | + | chr1.2:55208622-55208641 | MS.gene32049:CDS | 40.0% | |
| TAACAAAGATGAGGCTGAGA+AGG | + | chr1.2:55207296-55207315 | MS.gene32049:CDS | 40.0% | |
| TCAGGATCATTCTCATCCAA+AGG | - | chr1.2:55208650-55208669 | None:intergenic | 40.0% | |
| TCGCAGAGCTTTCATTAATG+GGG | + | chr1.2:55207542-55207561 | MS.gene32049:CDS | 40.0% | |
| TGCTGTGCAAATTTATGCCA+AGG | + | chr1.2:55207398-55207417 | MS.gene32049:CDS | 40.0% | |
| TGTTAGTTGAACGAATGGTC+AGG | + | chr1.2:55207229-55207248 | MS.gene32049:CDS | 40.0% | |
| TTGGATGAGAATGATCCTGA+AGG | + | chr1.2:55208650-55208669 | MS.gene32049:CDS | 40.0% | |
| ! | CTGGAAATTTTGGATATGGC+TGG | + | chr1.2:55208302-55208321 | MS.gene32049:CDS | 40.0% |
| ! | GAATTGAAAGATGAAGGTGC+AGG | + | chr1.2:55208413-55208432 | MS.gene32049:CDS | 40.0% |
| !! | AGGCTCCTTTTCAAAGTGTT+GGG | - | chr1.2:55207361-55207380 | None:intergenic | 40.0% |
| !! | GGGGAGTTGATATCTGAAAA+GGG | + | chr1.2:55207480-55207499 | MS.gene32049:CDS | 40.0% |
| !! | TGGGGAGTTGATATCTGAAA+AGG | + | chr1.2:55207479-55207498 | MS.gene32049:CDS | 40.0% |
| !! | TTAATTAGTAAGGCGCTGGA+AGG | + | chr1.2:55208437-55208456 | MS.gene32049:CDS | 40.0% |
| !! | TTTGTGATTTAAGGAGTGCC+CGG | - | chr1.2:55207854-55207873 | None:intergenic | 40.0% |
| !!! | GTTTTGAAAAGAGGACCAAG+AGG | + | chr1.2:55207444-55207463 | MS.gene32049:CDS | 40.0% |
| ACCTTAAGCTTCTCAGTGGA+AGG | - | chr1.2:55207951-55207970 | None:intergenic | 45.0% | |
| AGAAAATAGACGGAGTCGTC+AGG | - | chr1.2:55207257-55207276 | None:intergenic | 45.0% | |
| AGCCCTTGCTAATGCTTTGA+AGG | + | chr1.2:55208265-55208284 | MS.gene32049:CDS | 45.0% | |
| AGTGTTCGATGTTGGTACGA+CGG | - | chr1.2:55207168-55207187 | None:intergenic | 45.0% | |
| CACCCATGGCATTATTTGAC+AGG | - | chr1.2:55207812-55207831 | None:intergenic | 45.0% | |
| CACCGAAAATCTTCCAAAGC+TGG | - | chr1.2:55208044-55208063 | None:intergenic | 45.0% | |
| CAGAGCTTTCATTAATGGGG+AGG | + | chr1.2:55207545-55207564 | MS.gene32049:CDS | 45.0% | |
| CCGAAAATCTTCCAAAGCTG+GGG | - | chr1.2:55208042-55208061 | None:intergenic | 45.0% | |
| CCTTAAGCTTCTCAGTGGAA+GGG | - | chr1.2:55207950-55207969 | None:intergenic | 45.0% | |
| GGTCGCAGAGCTTTCATTAA+TGG | + | chr1.2:55207540-55207559 | MS.gene32049:CDS | 45.0% | |
| TAATGCCATGGGTGAAAAGG+GGG | + | chr1.2:55207818-55207837 | MS.gene32049:CDS | 45.0% | |
| TGAGAATGATCCTGAAGGAG+AGG | + | chr1.2:55208655-55208674 | MS.gene32049:CDS | 45.0% | |
| ! | AAAAGGAGCCTGATGGTGAT+GGG | + | chr1.2:55207370-55207389 | MS.gene32049:CDS | 45.0% |
| ! | AAAAGGGGGTTAGAGCATTC+CGG | + | chr1.2:55207832-55207851 | MS.gene32049:CDS | 45.0% |
| ! | CACTTTGAAAAGGAGCCTGA+TGG | + | chr1.2:55207363-55207382 | MS.gene32049:CDS | 45.0% |
| ! | CAGGCTCCTTTTCAAAGTGT+TGG | - | chr1.2:55207362-55207381 | None:intergenic | 45.0% |
| ! | TGCACCGTCACTGGAAATTT+TGG | + | chr1.2:55208292-55208311 | MS.gene32049:CDS | 45.0% |
| ! | TGCGACCCAACACTTTGAAA+AGG | + | chr1.2:55207353-55207372 | MS.gene32049:CDS | 45.0% |
| !! | AACATGTTTGGTGTGGAAGC+TGG | + | chr1.2:55208155-55208174 | MS.gene32049:CDS | 45.0% |
| !! | AACCTAGTACTCTGACTTGG+AGG | - | chr1.2:55207207-55207226 | None:intergenic | 45.0% |
| !!! | CCCCAGCTTTGGAAGATTTT+CGG | + | chr1.2:55208039-55208058 | MS.gene32049:CDS | 45.0% |
| AACAGCTTCTGCCAACAGCT+TGG | - | chr1.2:55208519-55208538 | None:intergenic | 50.0% | |
| AAGAGGGAAGGAGGAGAATG+GGG | + | chr1.2:55207461-55207480 | MS.gene32049:CDS | 50.0% | |
| CAAGAGGGAAGGAGGAGAAT+GGG | + | chr1.2:55207460-55207479 | MS.gene32049:CDS | 50.0% | |
| CCCTTCCACTGAGAAGCTTA+AGG | + | chr1.2:55207947-55207966 | MS.gene32049:CDS | 50.0% | |
| CGGTGTTCATCTACCAGAGT+AGG | + | chr1.2:55208059-55208078 | MS.gene32049:CDS | 50.0% | |
| GAAAAGAGGACCAAGAGGGA+AGG | + | chr1.2:55207449-55207468 | MS.gene32049:CDS | 50.0% | |
| GAAGCTGTTGTCCAGAAACC+TGG | + | chr1.2:55208530-55208549 | MS.gene32049:CDS | 50.0% | |
| TCATCAACGTCCTCTCCTTC+AGG | - | chr1.2:55208668-55208687 | None:intergenic | 50.0% | |
| TGAGGCTGAGAAGGATGCAA+AGG | + | chr1.2:55207305-55207324 | MS.gene32049:CDS | 50.0% | |
| TGTGAAACGTTCCCCAGCTT+TGG | + | chr1.2:55208028-55208047 | MS.gene32049:CDS | 50.0% | |
| ! | AAAGGGGGTTAGAGCATTCC+GGG | + | chr1.2:55207833-55207852 | MS.gene32049:CDS | 50.0% |
| ! | AGGAGAGGACGTTGATGAAG+AGG | + | chr1.2:55208670-55208689 | MS.gene32049:CDS | 50.0% |
| ! | GAAAAGGAGCCTGATGGTGA+TGG | + | chr1.2:55207369-55207388 | MS.gene32049:CDS | 50.0% |
| ! | GCGTGACAACATGTTTGGTG+TGG | + | chr1.2:55208148-55208167 | MS.gene32049:CDS | 50.0% |
| ! | TCTAACCCCCTTTTCACCCA+TGG | - | chr1.2:55207826-55207845 | None:intergenic | 50.0% |
| ! | TCTACCAGAGTAGGCTCTGA+AGG | + | chr1.2:55208068-55208087 | MS.gene32049:CDS | 50.0% |
| ! | TTGCTCTTGCTGAAGCGCTT+GGG | + | chr1.2:55208096-55208115 | MS.gene32049:CDS | 50.0% |
| AAGAGGACCAAGAGGGAAGG+AGG | + | chr1.2:55207452-55207471 | MS.gene32049:CDS | 55.0% | |
| ACCACCTTCAGAGCCTACTC+TGG | - | chr1.2:55208075-55208094 | None:intergenic | 55.0% | |
| ACCTGACATGCTGGGTCCTT+TGG | + | chr1.2:55208631-55208650 | MS.gene32049:CDS | 55.0% | |
| CCAAGAGGGAAGGAGGAGAA+TGG | + | chr1.2:55207459-55207478 | MS.gene32049:CDS | 55.0% | |
| CCATTCTCCTCCTTCCCTCT+TGG | - | chr1.2:55207462-55207481 | None:intergenic | 55.0% | |
| GAAGGAATCTGCACCGTCAC+TGG | + | chr1.2:55208283-55208302 | MS.gene32049:CDS | 55.0% | |
| GCAGAACTCCCATCACCATC+AGG | - | chr1.2:55207381-55207400 | None:intergenic | 55.0% | |
| TCCAAAGGACCCAGCATGTC+AGG | - | chr1.2:55208635-55208654 | None:intergenic | 55.0% | |
| ! | ATGGTCTGGAGCCAAGCTGT+TGG | + | chr1.2:55208505-55208524 | MS.gene32049:CDS | 55.0% |
| ! | GGCCTCCAAGTCAGAGTACT+AGG | + | chr1.2:55207202-55207221 | MS.gene32049:CDS | 55.0% |
| ! | GTTGCTCTTGCTGAAGCGCT+TGG | + | chr1.2:55208095-55208114 | MS.gene32049:CDS | 55.0% |
| ! | TGCTCTTGCTGAAGCGCTTG+GGG | + | chr1.2:55208097-55208116 | MS.gene32049:CDS | 55.0% |
| !! | ACCAGAGTAGGCTCTGAAGG+TGG | + | chr1.2:55208071-55208090 | MS.gene32049:CDS | 55.0% |
| !! | AGTAAGGCGCTGGAAGGTCG+TGG | + | chr1.2:55208443-55208462 | MS.gene32049:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 55207150 | 55208751 | 55207150 | ID=MS.gene32049 |
| chr1.2 | mRNA | 55207150 | 55208751 | 55207150 | ID=MS.gene32049.t1;Parent=MS.gene32049 |
| chr1.2 | exon | 55207150 | 55208751 | 55207150 | ID=MS.gene32049.t1.exon1;Parent=MS.gene32049.t1 |
| chr1.2 | CDS | 55207150 | 55208751 | 55207150 | ID=cds.MS.gene32049.t1;Parent=MS.gene32049.t1 |
| Gene Sequence |
| Protein sequence |
