Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66412.t1 | RHN52894.1 | 29 | 145 | 102 | 1 | 185 | 328 | 14 | 158 | 3.60E-11 | 79.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66412.t1 | O64517 | 33.6 | 122 | 66 | 4 | 137 | 248 | 28 | 144 | 1.4e-08 | 62.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66412.t1 | A0A392MHL9 | 25.4 | 213 | 150 | 4 | 122 | 327 | 36 | 246 | 2.6e-11 | 79.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene66412 | 0.823119 | 1.64E-53 | -1.69E-46 |
| MS.gene049272 | MS.gene66412 | 0.800171 | 1.63E-48 | -1.69E-46 |
| MS.gene049977 | MS.gene66412 | 0.824425 | 8.13E-54 | -1.69E-46 |
| MS.gene050026 | MS.gene66412 | 0.808941 | 2.42E-50 | -1.69E-46 |
| MS.gene052037 | MS.gene66412 | 0.807287 | 5.44E-50 | -1.69E-46 |
| MS.gene052447 | MS.gene66412 | 0.832294 | 1.03E-55 | -1.69E-46 |
| MS.gene052519 | MS.gene66412 | 0.809555 | 1.79E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene66412 | 0.806663 | 7.37E-50 | -1.69E-46 |
| MS.gene052586 | MS.gene66412 | 0.801714 | 7.90E-49 | -1.69E-46 |
| MS.gene053082 | MS.gene66412 | 0.810632 | 1.05E-50 | -1.69E-46 |
| MS.gene05329 | MS.gene66412 | 0.80141 | 9.12E-49 | -1.69E-46 |
| MS.gene053396 | MS.gene66412 | 0.818931 | 1.51E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene66412 | 0.803121 | 4.05E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene66412 | -0.801942 | 7.10E-49 | -1.69E-46 |
| MS.gene054991 | MS.gene66412 | 0.80661 | 7.57E-50 | -1.69E-46 |
| MS.gene056272 | MS.gene66412 | 0.826874 | 2.13E-54 | -1.69E-46 |
| MS.gene057021 | MS.gene66412 | 0.805254 | 1.46E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene66412 | 0.800557 | 1.36E-48 | -1.69E-46 |
| MS.gene058294 | MS.gene66412 | 0.807583 | 4.71E-50 | -1.69E-46 |
| MS.gene059272 | MS.gene66412 | 0.827468 | 1.54E-54 | -1.69E-46 |
| MS.gene060134 | MS.gene66412 | 0.805556 | 1.26E-49 | -1.69E-46 |
| MS.gene060521 | MS.gene66412 | 0.806431 | 8.25E-50 | -1.69E-46 |
| MS.gene060634 | MS.gene66412 | -0.801305 | 9.59E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66412.t1 | MTR_6g086745 | 29.496 | 139 | 84 | 4 | 131 | 263 | 3 | 133 | 4.76e-13 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66412.t1 | AT1G79340 | 33.607 | 122 | 66 | 4 | 137 | 248 | 28 | 144 | 4.42e-11 | 63.9 |
Find 85 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCCCTTGATCGAGCATATT+TGG | 0.101094 | 6.3:-82685586 | None:intergenic |
| ATTATAATTGATTGTTGTTA+TGG | 0.225008 | 6.3:+82688679 | MS.gene66412:CDS |
| TGATACCTGTTGAGCCTCTT+TGG | 0.238581 | 6.3:-82683248 | None:intergenic |
| GTACGCAGAATGAGATTTCT+TGG | 0.241203 | 6.3:-82687836 | None:intergenic |
| TGAACAGGTGCACCTTTATC+AGG | 0.262418 | 6.3:-82683181 | None:intergenic |
| AATACCACCATGGGCCTCTA+AGG | 0.300464 | 6.3:+82685522 | MS.gene66412:CDS |
| GCCCTTGATCGAGCATATTT+GGG | 0.333058 | 6.3:-82685585 | None:intergenic |
| AGTTTAGGACGAGTTCCTTT+AGG | 0.335305 | 6.3:-82685743 | None:intergenic |
| TTATAATTGATTGTTGTTAT+GGG | 0.369389 | 6.3:+82688680 | MS.gene66412:CDS |
| CCCTCAATCAATACCACGTT+TGG | 0.395097 | 6.3:-82688727 | None:intergenic |
| AGAGAGTAGACTTACAGTTT+AGG | 0.395478 | 6.3:-82685758 | None:intergenic |
| ACTTCATCAACAATTACTTC+AGG | 0.399980 | 6.3:-82688844 | None:intergenic |
| CAGAATGAGATTTCTTGGTT+TGG | 0.400328 | 6.3:-82687831 | None:intergenic |
| TGATGGATGATGGAGAGTTC+TGG | 0.418959 | 6.3:+82687806 | MS.gene66412:CDS |
| AATTACTTCAGGTGGCTTGT+TGG | 0.422147 | 6.3:-82688833 | None:intergenic |
| GTTCCTTTAGGACCTACTTC+AGG | 0.422882 | 6.3:-82685731 | None:intergenic |
| ACAATGTTGCAGCCTGATAA+AGG | 0.432140 | 6.3:+82683169 | MS.gene66412:intron |
| TACATCCGACACACCCTTAG+AGG | 0.434282 | 6.3:-82685536 | None:intergenic |
| TGGGTCCAAAGAGGCTCAAC+AGG | 0.434538 | 6.3:+82683243 | MS.gene66412:CDS |
| ATCCCAAATATGCTCGATCA+AGG | 0.457840 | 6.3:+82685583 | MS.gene66412:CDS |
| ATTTGTTTGTACAGAAAACT+TGG | 0.459858 | 6.3:+82687664 | MS.gene66412:intron |
| CAAAGGAGGCTATGGACAAA+GGG | 0.468217 | 6.3:+82688882 | MS.gene66412:CDS |
| GCTCCACATGGTGTAAAGTC+TGG | 0.482817 | 6.3:-82683484 | None:intergenic |
| GATTGAGGGATCACAGGAAT+CGG | 0.487787 | 6.3:+82688741 | MS.gene66412:CDS |
| TAAAGGTGCACCTGTTCAAA+AGG | 0.489639 | 6.3:+82683186 | MS.gene66412:CDS |
| GCTACAGATAGAATCATTGC+AGG | 0.492856 | 6.3:+82683505 | MS.gene66412:CDS |
| AGATTTAACAAAGGAGGCTA+TGG | 0.496496 | 6.3:+82688874 | MS.gene66412:CDS |
| CTGTTCAAAAGGTTGGTGAC+TGG | 0.502902 | 6.3:+82683197 | MS.gene66412:CDS |
| GAGGGTTGGAGTGGAAGTGA+TGG | 0.502942 | 6.3:+82688766 | MS.gene66412:CDS |
| TTACCTGAAGTAGGTCCTAA+AGG | 0.509479 | 6.3:+82685728 | MS.gene66412:CDS |
| TGTTCAAAAGGTTGGTGACT+GGG | 0.512523 | 6.3:+82683198 | MS.gene66412:CDS |
| GGAAAACATACATCTAATGA+TGG | 0.515944 | 6.3:+82687789 | MS.gene66412:CDS |
| CACAGGAATCGGAAGAGGGT+TGG | 0.516005 | 6.3:+82688752 | MS.gene66412:CDS |
| GCCAAACGTGGTATTGATTG+AGG | 0.518768 | 6.3:+82688726 | MS.gene66412:CDS |
| TACGAAGTAACCAATGAGCT+TGG | 0.524860 | 6.3:+82687856 | MS.gene66412:CDS |
| ATACATCTAATGATGGATGA+TGG | 0.526034 | 6.3:+82687796 | MS.gene66412:CDS |
| CCAAACGTGGTATTGATTGA+GGG | 0.527744 | 6.3:+82688727 | MS.gene66412:CDS |
| GGGATCACAGGAATCGGAAG+AGG | 0.540432 | 6.3:+82688747 | MS.gene66412:CDS |
| GGTAGGTTGTCGGTATCTAA+AGG | 0.544096 | 6.3:-82687698 | None:intergenic |
| TTGATCGAGCATATTTGGGA+TGG | 0.544744 | 6.3:-82685581 | None:intergenic |
| TGTAGCTCATGGTAGGTTGT+CGG | 0.545661 | 6.3:-82687708 | None:intergenic |
| GGTGCACCTGTTCAAAAGGT+TGG | 0.546662 | 6.3:+82683190 | MS.gene66412:CDS |
| GACTGGGACGTGAGAGAGGA+AGG | 0.547529 | 6.3:+82683214 | MS.gene66412:CDS |
| TATAATTGATTGTTGTTATG+GGG | 0.547967 | 6.3:+82688681 | MS.gene66412:CDS |
| GGGTACATCATCAGTCGGGA+AGG | 0.551995 | 6.3:-82685560 | None:intergenic |
| TCGTATATTGTCGCTTCAGA+TGG | 0.552322 | 6.3:+82687961 | MS.gene66412:CDS |
| TCATCAACAATTACTTCAGG+TGG | 0.558784 | 6.3:-82688841 | None:intergenic |
| ACAAAGGAGGCTATGGACAA+AGG | 0.561435 | 6.3:+82688881 | MS.gene66412:CDS |
| TGATCGAGCATATTTGGGAT+GGG | 0.562852 | 6.3:-82685580 | None:intergenic |
| ATAATTGATTGTTGTTATGG+GGG | 0.565003 | 6.3:+82688682 | MS.gene66412:CDS |
| ACTCGAGAATATAGCAACTT+CGG | 0.573386 | 6.3:+82682444 | MS.gene66412:CDS |
| TACCATGAGCTACACTCACA+TGG | 0.577412 | 6.3:+82687717 | MS.gene66412:CDS |
| GGTATTGATTGAGGGATCAC+AGG | 0.578790 | 6.3:+82688735 | MS.gene66412:CDS |
| TGGTGACTGGGACGTGAGAG+AGG | 0.582301 | 6.3:+82683210 | MS.gene66412:CDS |
| GGATGGGTACATCATCAGTC+GGG | 0.583726 | 6.3:-82685564 | None:intergenic |
| AATCAAAGATTTAACAAAGG+AGG | 0.588468 | 6.3:+82688868 | MS.gene66412:CDS |
| GGGATGGGTACATCATCAGT+CGG | 0.594509 | 6.3:-82685565 | None:intergenic |
| ATACCACCATGGGCCTCTAA+GGG | 0.599672 | 6.3:+82685523 | MS.gene66412:CDS |
| ATGAGCTACACTCACATGGA+TGG | 0.600029 | 6.3:+82687721 | MS.gene66412:CDS |
| TCCCAAATATGCTCGATCAA+GGG | 0.600491 | 6.3:+82685584 | MS.gene66412:CDS |
| ATGGGCCTCTAAGGGTGTGT+CGG | 0.601089 | 6.3:+82685531 | MS.gene66412:CDS |
| TTTAGCAGGGCAAATGAAGG+AGG | 0.602852 | 6.3:+82687882 | MS.gene66412:CDS |
| GCTAGGCTCTGGAGTTACAA+AGG | 0.603598 | 6.3:+82685697 | MS.gene66412:intron |
| GAATCGGAAGAGGGTTGGAG+TGG | 0.604625 | 6.3:+82688757 | MS.gene66412:CDS |
| GCAGGGCAAATGAAGGAGGA+TGG | 0.608543 | 6.3:+82687886 | MS.gene66412:CDS |
| AATATGCTCGATCAAGGGCA+AGG | 0.621666 | 6.3:+82685589 | MS.gene66412:CDS |
| GTGAGAGAGGAAGGACAGCG+TGG | 0.623214 | 6.3:+82683223 | MS.gene66412:CDS |
| GATGCTGAGTTACCTGAAGT+AGG | 0.623391 | 6.3:+82685719 | MS.gene66412:CDS |
| GGTACATCATCAGTCGGGAA+GGG | 0.628274 | 6.3:-82685559 | None:intergenic |
| ATCCATGTGAGTGTAGCTCA+TGG | 0.631332 | 6.3:-82687719 | None:intergenic |
| GGATCACAGGAATCGGAAGA+GGG | 0.634702 | 6.3:+82688748 | MS.gene66412:CDS |
| CAGATTCGAGAATACCACCA+TGG | 0.636690 | 6.3:+82685512 | MS.gene66412:intron |
| ATTCTATCTGTAGCTCCACA+TGG | 0.639438 | 6.3:-82683496 | None:intergenic |
| GGACACGGCTCCAGAGAGAA+CGG | 0.643752 | 6.3:+82687934 | MS.gene66412:CDS |
| AGATTCGAGAATACCACCAT+GGG | 0.651983 | 6.3:+82685513 | MS.gene66412:intron |
| ATGTGAGTGTAGCTCATGGT+AGG | 0.655223 | 6.3:-82687715 | None:intergenic |
| GACACACCCTTAGAGGCCCA+TGG | 0.657331 | 6.3:-82685529 | None:intergenic |
| AGAGATTTGTGCGCCAAACG+TGG | 0.658136 | 6.3:+82688714 | MS.gene66412:CDS |
| AGGACAGCGTGGGTCCAAAG+AGG | 0.658490 | 6.3:+82683234 | MS.gene66412:CDS |
| TGAGAGAGGAAGGACAGCGT+GGG | 0.669850 | 6.3:+82683224 | MS.gene66412:CDS |
| GTAAATCAAAGATTTAACAA+AGG | 0.670683 | 6.3:+82688865 | MS.gene66412:CDS |
| ATATACACTGAACAATGTGG+TGG | 0.686889 | 6.3:-82688658 | None:intergenic |
| TATCCAGACTTTACACCATG+TGG | 0.695282 | 6.3:+82683481 | MS.gene66412:CDS |
| ATAATATACACTGAACAATG+TGG | 0.701915 | 6.3:-82688661 | None:intergenic |
| ACACCCTTAGAGGCCCATGG+TGG | 0.767993 | 6.3:-82685526 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 82681948 | 82688954 | 82681948 | ID=MS.gene66412 |
| chr6.3 | mRNA | 82681948 | 82688954 | 82681948 | ID=MS.gene66412.t1;Parent=MS.gene66412 |
| chr6.3 | exon | 82681948 | 82681950 | 82681948 | ID=MS.gene66412.t1.exon1;Parent=MS.gene66412.t1 |
| chr6.3 | CDS | 82681948 | 82681950 | 82681948 | ID=cds.MS.gene66412.t1;Parent=MS.gene66412.t1 |
| chr6.3 | exon | 82682436 | 82682465 | 82682436 | ID=MS.gene66412.t1.exon2;Parent=MS.gene66412.t1 |
| chr6.3 | CDS | 82682436 | 82682465 | 82682436 | ID=cds.MS.gene66412.t1;Parent=MS.gene66412.t1 |
| chr6.3 | exon | 82683181 | 82683264 | 82683181 | ID=MS.gene66412.t1.exon3;Parent=MS.gene66412.t1 |
| chr6.3 | CDS | 82683181 | 82683264 | 82683181 | ID=cds.MS.gene66412.t1;Parent=MS.gene66412.t1 |
| chr6.3 | exon | 82683472 | 82683532 | 82683472 | ID=MS.gene66412.t1.exon4;Parent=MS.gene66412.t1 |
| chr6.3 | CDS | 82683472 | 82683532 | 82683472 | ID=cds.MS.gene66412.t1;Parent=MS.gene66412.t1 |
| chr6.3 | exon | 82685515 | 82685610 | 82685515 | ID=MS.gene66412.t1.exon5;Parent=MS.gene66412.t1 |
| chr6.3 | CDS | 82685515 | 82685610 | 82685515 | ID=cds.MS.gene66412.t1;Parent=MS.gene66412.t1 |
| chr6.3 | exon | 82685702 | 82685765 | 82685702 | ID=MS.gene66412.t1.exon6;Parent=MS.gene66412.t1 |
| chr6.3 | CDS | 82685702 | 82685765 | 82685702 | ID=cds.MS.gene66412.t1;Parent=MS.gene66412.t1 |
| chr6.3 | exon | 82687678 | 82687997 | 82687678 | ID=MS.gene66412.t1.exon7;Parent=MS.gene66412.t1 |
| chr6.3 | CDS | 82687678 | 82687997 | 82687678 | ID=cds.MS.gene66412.t1;Parent=MS.gene66412.t1 |
| chr6.3 | exon | 82688626 | 82688954 | 82688626 | ID=MS.gene66412.t1.exon8;Parent=MS.gene66412.t1 |
| chr6.3 | CDS | 82688626 | 82688954 | 82688626 | ID=cds.MS.gene66412.t1;Parent=MS.gene66412.t1 |
| Gene Sequence |
| Protein sequence |
