Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66682.t1 | KEH29799.1 | 81.5 | 372 | 48 | 4 | 2 | 371 | 115 | 467 | 2.40E-160 | 575.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66682.t1 | Q9C641 | 46.5 | 368 | 162 | 4 | 12 | 371 | 143 | 483 | 1.1e-86 | 321.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66682.t1 | A0A072UJ74 | 81.5 | 372 | 48 | 4 | 2 | 371 | 115 | 467 | 1.7e-160 | 575.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049531 | MS.gene66682 | 0.812117 | 4.98E-51 | -1.69E-46 |
| MS.gene049701 | MS.gene66682 | 0.805126 | 1.55E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene66682 | 0.800052 | 1.73E-48 | -1.69E-46 |
| MS.gene050221 | MS.gene66682 | 0.820903 | 5.36E-53 | -1.69E-46 |
| MS.gene051594 | MS.gene66682 | 0.809065 | 2.27E-50 | -1.69E-46 |
| MS.gene051710 | MS.gene66682 | 0.811653 | 6.29E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene66682 | 0.8053 | 1.43E-49 | -1.69E-46 |
| MS.gene053512 | MS.gene66682 | 0.812883 | 3.39E-51 | -1.69E-46 |
| MS.gene05422 | MS.gene66682 | 0.83114 | 1.98E-55 | -1.69E-46 |
| MS.gene05423 | MS.gene66682 | 0.819438 | 1.16E-52 | -1.69E-46 |
| MS.gene054291 | MS.gene66682 | -0.805135 | 1.55E-49 | -1.69E-46 |
| MS.gene054775 | MS.gene66682 | 0.806797 | 6.91E-50 | -1.69E-46 |
| MS.gene054801 | MS.gene66682 | 0.822069 | 2.88E-53 | -1.69E-46 |
| MS.gene055056 | MS.gene66682 | 0.825634 | 4.21E-54 | -1.69E-46 |
| MS.gene055058 | MS.gene66682 | 0.811114 | 8.23E-51 | -1.69E-46 |
| MS.gene05531 | MS.gene66682 | 0.804431 | 2.17E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene66682 | 0.801068 | 1.07E-48 | -1.69E-46 |
| MS.gene058515 | MS.gene66682 | 0.885225 | 9.77E-72 | -1.69E-46 |
| MS.gene05925 | MS.gene66682 | 0.803873 | 2.83E-49 | -1.69E-46 |
| MS.gene059276 | MS.gene66682 | 0.855246 | 7.17E-62 | -1.69E-46 |
| MS.gene059584 | MS.gene66682 | 0.827601 | 1.43E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66682.t1 | MTR_4g051702 | 80.749 | 374 | 49 | 4 | 1 | 371 | 114 | 467 | 0.0 | 593 |
| MS.gene66682.t1 | MTR_3g014380 | 77.984 | 377 | 62 | 7 | 3 | 371 | 118 | 481 | 0.0 | 566 |
| MS.gene66682.t1 | MTR_6g023380 | 81.734 | 323 | 55 | 3 | 50 | 371 | 1 | 320 | 0.0 | 533 |
| MS.gene66682.t1 | MTR_8g105060 | 70.028 | 357 | 75 | 6 | 13 | 359 | 119 | 453 | 2.88e-160 | 457 |
| MS.gene66682.t1 | MTR_8g104250 | 73.574 | 333 | 66 | 7 | 36 | 359 | 93 | 412 | 4.72e-160 | 455 |
| MS.gene66682.t1 | MTR_8g105090 | 84.234 | 222 | 32 | 1 | 2 | 220 | 104 | 325 | 3.29e-130 | 376 |
| MS.gene66682.t1 | MTR_2g020660 | 48.509 | 369 | 154 | 5 | 12 | 371 | 133 | 474 | 2.09e-110 | 339 |
| MS.gene66682.t1 | MTR_7g044770 | 47.826 | 368 | 157 | 5 | 12 | 371 | 140 | 480 | 4.63e-110 | 338 |
| MS.gene66682.t1 | MTR_7g044770 | 47.576 | 330 | 138 | 5 | 50 | 371 | 1 | 303 | 5.49e-96 | 297 |
| MS.gene66682.t1 | MTR_2g029125 | 46.518 | 359 | 145 | 6 | 14 | 353 | 155 | 485 | 7.43e-94 | 289 |
| MS.gene66682.t1 | MTR_2g029125 | 46.049 | 367 | 141 | 7 | 14 | 353 | 155 | 491 | 4.43e-93 | 287 |
| MS.gene66682.t1 | MTR_2g029120 | 45.553 | 371 | 151 | 10 | 1 | 356 | 87 | 421 | 4.03e-92 | 283 |
| MS.gene66682.t1 | MTR_2g029120 | 45.026 | 382 | 163 | 11 | 1 | 356 | 87 | 447 | 4.36e-92 | 285 |
| MS.gene66682.t1 | MTR_2g029120 | 45.553 | 371 | 151 | 10 | 1 | 356 | 87 | 421 | 5.72e-92 | 283 |
| MS.gene66682.t1 | MTR_2g028350 | 42.278 | 395 | 159 | 7 | 9 | 363 | 126 | 491 | 3.54e-88 | 275 |
| MS.gene66682.t1 | MTR_2g028380 | 42.520 | 381 | 151 | 6 | 13 | 353 | 114 | 466 | 5.36e-88 | 273 |
| MS.gene66682.t1 | MTR_8g105040 | 66.368 | 223 | 30 | 4 | 149 | 371 | 1 | 178 | 5.34e-86 | 258 |
| MS.gene66682.t1 | MTR_2g067200 | 43.988 | 341 | 153 | 7 | 15 | 353 | 169 | 473 | 1.31e-83 | 263 |
| MS.gene66682.t1 | MTR_2g029125 | 44.737 | 342 | 142 | 6 | 14 | 336 | 155 | 468 | 8.81e-83 | 260 |
| MS.gene66682.t1 | MTR_2g029125 | 43.909 | 353 | 143 | 6 | 14 | 339 | 155 | 479 | 3.07e-82 | 260 |
| MS.gene66682.t1 | MTR_2g029125 | 44.286 | 350 | 138 | 7 | 14 | 336 | 155 | 474 | 4.69e-82 | 258 |
| MS.gene66682.t1 | MTR_2g029125 | 43.714 | 350 | 142 | 6 | 14 | 336 | 155 | 476 | 1.34e-81 | 257 |
| MS.gene66682.t1 | MTR_2g029125 | 43.714 | 350 | 142 | 6 | 14 | 336 | 155 | 476 | 3.68e-81 | 258 |
| MS.gene66682.t1 | MTR_2g029120 | 45.253 | 316 | 131 | 8 | 1 | 302 | 87 | 374 | 5.44e-78 | 245 |
| MS.gene66682.t1 | MTR_2g028400 | 41.470 | 381 | 163 | 7 | 14 | 363 | 140 | 491 | 1.98e-76 | 244 |
| MS.gene66682.t1 | MTR_2g028440 | 41.358 | 324 | 130 | 5 | 11 | 296 | 146 | 447 | 4.09e-69 | 227 |
| MS.gene66682.t1 | MTR_2g028360 | 35.938 | 384 | 176 | 9 | 12 | 353 | 222 | 577 | 2.99e-62 | 210 |
| MS.gene66682.t1 | MTR_2g028360 | 46.364 | 220 | 98 | 2 | 13 | 231 | 10 | 210 | 5.02e-57 | 196 |
| MS.gene66682.t1 | MTR_2g028340 | 44.444 | 234 | 110 | 2 | 11 | 243 | 178 | 392 | 2.26e-61 | 202 |
| MS.gene66682.t1 | MTR_2g066890 | 42.570 | 249 | 122 | 3 | 15 | 256 | 16 | 250 | 4.03e-61 | 197 |
| MS.gene66682.t1 | MTR_4g101750 | 29.513 | 349 | 212 | 9 | 12 | 354 | 167 | 487 | 3.52e-38 | 145 |
| MS.gene66682.t1 | MTR_4g101750 | 29.513 | 349 | 212 | 9 | 12 | 354 | 167 | 487 | 4.41e-38 | 145 |
| MS.gene66682.t1 | MTR_8g105070 | 77.778 | 90 | 11 | 2 | 264 | 353 | 5 | 85 | 2.81e-37 | 131 |
| MS.gene66682.t1 | MTR_5g083100 | 41.553 | 219 | 74 | 3 | 158 | 371 | 265 | 434 | 3.18e-37 | 139 |
| MS.gene66682.t1 | MTR_2g067190 | 42.197 | 173 | 86 | 1 | 15 | 187 | 129 | 287 | 4.51e-37 | 135 |
| MS.gene66682.t1 | MTR_2g028300 | 41.892 | 148 | 66 | 2 | 14 | 160 | 78 | 206 | 6.21e-32 | 119 |
| MS.gene66682.t1 | MTR_2g029210 | 49.558 | 113 | 43 | 4 | 255 | 362 | 38 | 141 | 4.52e-22 | 93.2 |
| MS.gene66682.t1 | MTR_2g021910 | 34.104 | 173 | 72 | 2 | 78 | 250 | 413 | 543 | 6.33e-20 | 92.0 |
| MS.gene66682.t1 | MTR_2g021910 | 31.250 | 176 | 90 | 4 | 33 | 208 | 15 | 159 | 2.51e-16 | 80.9 |
| MS.gene66682.t1 | MTR_2g066880 | 52.041 | 98 | 33 | 4 | 265 | 362 | 5 | 88 | 6.22e-19 | 83.2 |
| MS.gene66682.t1 | MTR_2g101810 | 39.062 | 128 | 65 | 4 | 236 | 354 | 1 | 124 | 4.55e-18 | 80.9 |
| MS.gene66682.t1 | MTR_8g104240 | 60.784 | 51 | 20 | 0 | 321 | 371 | 33 | 83 | 1.26e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66682.t1 | AT1G45332 | 47.011 | 368 | 160 | 5 | 12 | 371 | 143 | 483 | 2.71e-108 | 334 |
| MS.gene66682.t1 | AT2G45030 | 47.011 | 368 | 160 | 5 | 12 | 371 | 143 | 483 | 5.04e-108 | 333 |
| MS.gene66682.t1 | AT1G45332 | 45.758 | 330 | 144 | 4 | 50 | 371 | 1 | 303 | 7.12e-94 | 291 |
| MS.gene66682.t1 | AT1G62750 | 28.940 | 349 | 214 | 9 | 12 | 354 | 171 | 491 | 2.66e-39 | 149 |
Find 50 sgRNAs with CRISPR-Local
Find 145 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGTTCTTTCTTGTCTTGA+TGG | 0.141370 | 4.3:+48214509 | MS.gene66682:CDS |
| TGAGGCGTTAGAACTATTTA+TGG | 0.231813 | 4.3:+48215045 | MS.gene66682:CDS |
| AACTTATCTTAGAATCTATC+AGG | 0.249865 | 4.3:+48215222 | MS.gene66682:CDS |
| TGTTGATGTTCCTGCTTATT+CGG | 0.281836 | 4.3:+48215468 | MS.gene66682:CDS |
| CTCTCATCACCAATGATAAT+TGG | 0.285305 | 4.3:-48215019 | None:intergenic |
| TGTTGATCTTGTCAAGTTAG+AGG | 0.299617 | 4.3:+48214724 | MS.gene66682:CDS |
| TTTGATGCTCATCTTGAATC+TGG | 0.300024 | 4.3:+48215403 | MS.gene66682:CDS |
| TATTTATGGAGGGTGTAATT+AGG | 0.307222 | 4.3:+48215059 | MS.gene66682:CDS |
| GAAGATTCAGATTCCTCGTT+TGG | 0.326066 | 4.3:+48215291 | MS.gene66682:CDS |
| CACATTGACGATTGTTATGT+TGG | 0.346186 | 4.3:+48214698 | MS.gene66682:CDS |
| GCTTATATTTATTGACGATC+TGG | 0.368085 | 4.3:+48214589 | MS.gene66682:CDS |
| CACAAAGGCTTGCATATCTT+CGG | 0.381625 | 4.3:-48214855 | None:intergenic |
| GCAAAAGCCTTTGCAACAAA+TGG | 0.400513 | 4.3:-48215172 | None:intergenic |
| ATCGGGAAGGAGCAGATCTA+TGG | 0.411943 | 4.3:+48214612 | MS.gene66682:CDS |
| AAGATTCAGATTCCTCGTTT+GGG | 0.414539 | 4.3:+48215292 | MS.gene66682:CDS |
| TAAAACCGAATCCATTACTA+TGG | 0.416829 | 4.3:+48214535 | MS.gene66682:CDS |
| AAATTTGTACCAATTATCAT+TGG | 0.426791 | 4.3:+48215010 | MS.gene66682:CDS |
| TTGATCCATAGTAATGGATT+CGG | 0.433471 | 4.3:-48214540 | None:intergenic |
| ACTTCTACATCATTATAATC+AGG | 0.443013 | 4.3:-48214821 | None:intergenic |
| TAACAATCGTCAATGTGTAT+TGG | 0.446757 | 4.3:-48214692 | None:intergenic |
| TGACAAGATTTCAACTTCCT+AGG | 0.454857 | 4.3:+48214567 | MS.gene66682:CDS |
| GGTGAGAAAGTTGAATTGTC+TGG | 0.460058 | 4.3:+48215136 | MS.gene66682:CDS |
| CATTTGTTGATCCATAGTAA+TGG | 0.470087 | 4.3:-48214546 | None:intergenic |
| AAAGTCTAACAAACATGTTA+AGG | 0.481772 | 4.3:-48214760 | None:intergenic |
| ATTTATTGACGATCTGGATC+GGG | 0.488083 | 4.3:+48214595 | MS.gene66682:CDS |
| TGATGCACCATTTGTTGCAA+AGG | 0.499606 | 4.3:+48215165 | MS.gene66682:CDS |
| GCTCTTGATCTAAATAAGAA+TGG | 0.506175 | 4.3:+48215115 | MS.gene66682:CDS |
| CTATCAGGGTGTTATTAAGA+AGG | 0.508785 | 4.3:+48215237 | MS.gene66682:CDS |
| TATTTATTGACGATCTGGAT+CGG | 0.519096 | 4.3:+48214594 | MS.gene66682:CDS |
| ACCATTTGAAAATGTCTGCC+CGG | 0.527437 | 4.3:-48215503 | None:intergenic |
| ACTCATTGAAATTGTATCAG+AGG | 0.540690 | 4.3:+48214895 | MS.gene66682:CDS |
| TTACTAACATCGATTGGACT+TGG | 0.543188 | 4.3:-48215088 | None:intergenic |
| TTTATTACAAATGTTAACAC+TGG | 0.545461 | 4.3:+48215265 | MS.gene66682:CDS |
| GAAGTTGATGAAGCTCATGC+TGG | 0.546282 | 4.3:+48215364 | MS.gene66682:CDS |
| GCATAATTACTAACATCGAT+TGG | 0.547196 | 4.3:-48215094 | None:intergenic |
| GATTATAATGATGTAGAAGT+AGG | 0.547209 | 4.3:+48214824 | MS.gene66682:CDS |
| ACAAAGGCTTGCATATCTTC+GGG | 0.570024 | 4.3:-48214854 | None:intergenic |
| GGCGTTAGAACTATTTATGG+AGG | 0.572694 | 4.3:+48215048 | MS.gene66682:CDS |
| GCATCATCGAGAACAGACTC+TGG | 0.573694 | 4.3:-48214962 | None:intergenic |
| CATTGGTGATGAGAGCTATG+AGG | 0.582206 | 4.3:+48215027 | MS.gene66682:CDS |
| TCGTCAATAAATATAAGCCT+AGG | 0.585899 | 4.3:-48214584 | None:intergenic |
| TTCTCGATGATGCAATTCGT+AGG | 0.597250 | 4.3:+48214972 | MS.gene66682:CDS |
| TATCAGGGTGTTATTAAGAA+GGG | 0.606667 | 4.3:+48215238 | MS.gene66682:CDS |
| TCTCGATGATGCAATTCGTA+GGG | 0.618739 | 4.3:+48214973 | MS.gene66682:CDS |
| GCGTTAGAACTATTTATGGA+GGG | 0.620519 | 4.3:+48215049 | MS.gene66682:CDS |
| TGTATATCTAACAGACCCGT+CGG | 0.628384 | 4.3:-48215437 | None:intergenic |
| ACTTATCTTAGAATCTATCA+GGG | 0.629324 | 4.3:+48215223 | MS.gene66682:CDS |
| TGAGCAAGTTAATAAAATCG+AGG | 0.634586 | 4.3:+48215342 | MS.gene66682:CDS |
| TCACGTCTCTTGCCCAAACG+AGG | 0.652892 | 4.3:-48215304 | None:intergenic |
| ATTGACGATCTGGATCGGGA+AGG | 0.672041 | 4.3:+48214599 | MS.gene66682:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAACTATTAATTCAATAATT+TGG | + | chr4.3:48213896-48213915 | MS.gene66682:intron | 10.0% |
| !!! | AAATGATATTTTCTAAAATA+CGG | - | chr4.3:48213671-48213690 | None:intergenic | 10.0% |
| !!! | TTTCTAAAAAATATAAATTC+TGG | + | chr4.3:48213365-48213384 | MS.gene66682:intron | 10.0% |
| !! | AAAGAATGTTAATATTAGAA+AGG | + | chr4.3:48213965-48213984 | MS.gene66682:intron | 15.0% |
| !! | AAGAATGTTAATATTAGAAA+GGG | + | chr4.3:48213966-48213985 | MS.gene66682:intron | 15.0% |
| !! | TTCTAATATTAACATTCTTT+CGG | - | chr4.3:48213965-48213984 | None:intergenic | 15.0% |
| !! | AAATTTGTACCAATTATCAT+TGG | + | chr4.3:48215010-48215029 | MS.gene66682:CDS | 20.0% |
| !! | TCTAATATTAACATTCTTTC+GGG | - | chr4.3:48213964-48213983 | None:intergenic | 20.0% |
| !! | TTTATTACAAATGTTAACAC+TGG | + | chr4.3:48215265-48215284 | MS.gene66682:CDS | 20.0% |
| !!! | AAATATGGTTCTGTTTTTAT+AGG | - | chr4.3:48214387-48214406 | None:intergenic | 20.0% |
| !!! | TAGACTTTTTGATAAGTTAA+AGG | + | chr4.3:48214775-48214794 | MS.gene66682:CDS | 20.0% |
| ! | AAAGTCTAACAAACATGTTA+AGG | - | chr4.3:48214763-48214782 | None:intergenic | 25.0% |
| ! | AACTTATCTTAGAATCTATC+AGG | + | chr4.3:48215222-48215241 | MS.gene66682:CDS | 25.0% |
| ! | ACTTATCTTAGAATCTATCA+GGG | + | chr4.3:48215223-48215242 | MS.gene66682:CDS | 25.0% |
| ! | ACTTCTACATCATTATAATC+AGG | - | chr4.3:48214824-48214843 | None:intergenic | 25.0% |
| ! | GATTATAATGATGTAGAAGT+AGG | + | chr4.3:48214824-48214843 | MS.gene66682:CDS | 25.0% |
| ! | TAAGCAACAAATGTCAAATA+TGG | - | chr4.3:48214402-48214421 | None:intergenic | 25.0% |
| !! | ACCAATACACTCAAATTTTT+GGG | + | chr4.3:48213922-48213941 | MS.gene66682:intron | 25.0% |
| !! | TACCAATACACTCAAATTTT+TGG | + | chr4.3:48213921-48213940 | MS.gene66682:intron | 25.0% |
| !!! | AAACTTTCTAAAGCTTTTCA+TGG | + | chr4.3:48214926-48214945 | MS.gene66682:CDS | 25.0% |
| !!! | TGTTGATAATTATGATGACA+TGG | - | chr4.3:48213506-48213525 | None:intergenic | 25.0% |
| ACCCAAAAATTTGAGTGTAT+TGG | - | chr4.3:48213926-48213945 | None:intergenic | 30.0% | |
| ACCTAACAAACATTAAGATG+CGG | - | chr4.3:48214019-48214038 | None:intergenic | 30.0% | |
| ACTCATTGAAATTGTATCAG+AGG | + | chr4.3:48214895-48214914 | MS.gene66682:CDS | 30.0% | |
| GCATAATTACTAACATCGAT+TGG | - | chr4.3:48215097-48215116 | None:intergenic | 30.0% | |
| GCTCTTGATCTAAATAAGAA+TGG | + | chr4.3:48215115-48215134 | MS.gene66682:CDS | 30.0% | |
| GCTTATATTTATTGACGATC+TGG | + | chr4.3:48214589-48214608 | MS.gene66682:CDS | 30.0% | |
| TAAAACCGAATCCATTACTA+TGG | + | chr4.3:48214535-48214554 | MS.gene66682:CDS | 30.0% | |
| TAACAATCGTCAATGTGTAT+TGG | - | chr4.3:48214695-48214714 | None:intergenic | 30.0% | |
| TATTCGGTCTCAAAAAATTC+CGG | + | chr4.3:48215484-48215503 | MS.gene66682:CDS | 30.0% | |
| TATTTATTGACGATCTGGAT+CGG | + | chr4.3:48214594-48214613 | MS.gene66682:CDS | 30.0% | |
| TCGTCAATAAATATAAGCCT+AGG | - | chr4.3:48214587-48214606 | None:intergenic | 30.0% | |
| TGAGCAAGTTAATAAAATCG+AGG | + | chr4.3:48215342-48215361 | MS.gene66682:CDS | 30.0% | |
| ! | CATTTGTTGATCCATAGTAA+TGG | - | chr4.3:48214549-48214568 | None:intergenic | 30.0% |
| ! | TATTTATGGAGGGTGTAATT+AGG | + | chr4.3:48215059-48215078 | MS.gene66682:CDS | 30.0% |
| !! | TATCAGGGTGTTATTAAGAA+GGG | + | chr4.3:48215238-48215257 | MS.gene66682:CDS | 30.0% |
| !! | TTGATCCATAGTAATGGATT+CGG | - | chr4.3:48214543-48214562 | None:intergenic | 30.0% |
| !! | TTGTTGCTTAGATACTTTTG+TGG | + | chr4.3:48214412-48214431 | MS.gene66682:intron | 30.0% |
| !!! | ATGGTTCTGTTTTTATAGGA+AGG | - | chr4.3:48214383-48214402 | None:intergenic | 30.0% |
| !!! | GATTTTTGAAGTAAGAGTAC+TGG | - | chr4.3:48213600-48213619 | None:intergenic | 30.0% |
| !!! | TGGTTCTGTTTTTATAGGAA+GGG | - | chr4.3:48214382-48214401 | None:intergenic | 30.0% |
| AAGATTCAGATTCCTCGTTT+GGG | + | chr4.3:48215292-48215311 | MS.gene66682:CDS | 35.0% | |
| ATAGGAAGGGACATATGTTT+CGG | - | chr4.3:48214369-48214388 | None:intergenic | 35.0% | |
| ATTCGGTCTCAAAAAATTCC+GGG | + | chr4.3:48215485-48215504 | MS.gene66682:CDS | 35.0% | |
| ATTCTTTCGGGCAAATTGTA+TGG | - | chr4.3:48213952-48213971 | None:intergenic | 35.0% | |
| ATTTATTGACGATCTGGATC+GGG | + | chr4.3:48214595-48214614 | MS.gene66682:CDS | 35.0% | |
| CACATTGACGATTGTTATGT+TGG | + | chr4.3:48214698-48214717 | MS.gene66682:CDS | 35.0% | |
| CATGTTAAGGCGAAAAAAGT+AGG | - | chr4.3:48214750-48214769 | None:intergenic | 35.0% | |
| CTCTCATCACCAATGATAAT+TGG | - | chr4.3:48215022-48215041 | None:intergenic | 35.0% | |
| CTTGTTCTTTCTTGTCTTGA+TGG | + | chr4.3:48214509-48214528 | MS.gene66682:CDS | 35.0% | |
| GCGTTAGAACTATTTATGGA+GGG | + | chr4.3:48215049-48215068 | MS.gene66682:CDS | 35.0% | |
| TGACAAGATTTCAACTTCCT+AGG | + | chr4.3:48214567-48214586 | MS.gene66682:CDS | 35.0% | |
| TGTTGATCTTGTCAAGTTAG+AGG | + | chr4.3:48214724-48214743 | MS.gene66682:CDS | 35.0% | |
| TGTTGATGTTCCTGCTTATT+CGG | + | chr4.3:48215468-48215487 | MS.gene66682:CDS | 35.0% | |
| TGTTTCAGAGCGAAAAAAGT+AGG | - | chr4.3:48214801-48214820 | None:intergenic | 35.0% | |
| TTTGATGCTCATCTTGAATC+TGG | + | chr4.3:48215403-48215422 | MS.gene66682:CDS | 35.0% | |
| ! | TGAGGCGTTAGAACTATTTA+TGG | + | chr4.3:48215045-48215064 | MS.gene66682:CDS | 35.0% |
| ! | TTACTAACATCGATTGGACT+TGG | - | chr4.3:48215091-48215110 | None:intergenic | 35.0% |
| ! | TTGCAAAGGCTTTTGCAAAT+TGG | + | chr4.3:48215179-48215198 | MS.gene66682:CDS | 35.0% |
| !! | CTATCAGGGTGTTATTAAGA+AGG | + | chr4.3:48215237-48215256 | MS.gene66682:CDS | 35.0% |
| !!! | CTTTCTAAAGCTTTTCATGG+TGG | + | chr4.3:48214929-48214948 | MS.gene66682:CDS | 35.0% |
| !!! | TTTTTTGAGACCGAATAAGC+AGG | - | chr4.3:48215481-48215500 | None:intergenic | 35.0% |
| AAACATTAAGATGCGGCAAG+GGG | - | chr4.3:48214012-48214031 | None:intergenic | 40.0% | |
| ACAAACATTAAGATGCGGCA+AGG | - | chr4.3:48214014-48214033 | None:intergenic | 40.0% | |
| ACAAAGGCTTGCATATCTTC+GGG | - | chr4.3:48214857-48214876 | None:intergenic | 40.0% | |
| ACCATTTGAAAATGTCTGCC+CGG | - | chr4.3:48215506-48215525 | None:intergenic | 40.0% | |
| ACGGTACAACCGCAAAATTA+AGG | + | chr4.3:48213570-48213589 | MS.gene66682:intron | 40.0% | |
| AGATGGATAAATAGAGGAGG+CGG | - | chr4.3:48214147-48214166 | None:intergenic | 40.0% | |
| CAAACATTAAGATGCGGCAA+GGG | - | chr4.3:48214013-48214032 | None:intergenic | 40.0% | |
| CACAAAGGCTTGCATATCTT+CGG | - | chr4.3:48214858-48214877 | None:intergenic | 40.0% | |
| CCATGAATCGTTTCTTCTCA+AGG | + | chr4.3:48214078-48214097 | MS.gene66682:intron | 40.0% | |
| CCTTGAGAAGAAACGATTCA+TGG | - | chr4.3:48214081-48214100 | None:intergenic | 40.0% | |
| CGGTACAACCGCAAAATTAA+GGG | + | chr4.3:48213571-48213590 | MS.gene66682:intron | 40.0% | |
| GAAGATTCAGATTCCTCGTT+TGG | + | chr4.3:48215291-48215310 | MS.gene66682:CDS | 40.0% | |
| GCAAAAGCCTTTGCAACAAA+TGG | - | chr4.3:48215175-48215194 | None:intergenic | 40.0% | |
| GCCGCATCTTAATGTTTGTT+AGG | + | chr4.3:48214015-48214034 | MS.gene66682:intron | 40.0% | |
| GGAAGATGGATAAATAGAGG+AGG | - | chr4.3:48214150-48214169 | None:intergenic | 40.0% | |
| GGAGGAAGATGGATAAATAG+AGG | - | chr4.3:48214153-48214172 | None:intergenic | 40.0% | |
| GGCGTTAGAACTATTTATGG+AGG | + | chr4.3:48215048-48215067 | MS.gene66682:CDS | 40.0% | |
| GGTACAACCGCAAAATTAAG+GGG | + | chr4.3:48213572-48213591 | MS.gene66682:intron | 40.0% | |
| GGTGAGAAAGTTGAATTGTC+TGG | + | chr4.3:48215136-48215155 | MS.gene66682:CDS | 40.0% | |
| TCTCGATGATGCAATTCGTA+GGG | + | chr4.3:48214973-48214992 | MS.gene66682:CDS | 40.0% | |
| TGAGAAGAAACGATTCATGG+CGG | - | chr4.3:48214078-48214097 | None:intergenic | 40.0% | |
| TGTATATCTAACAGACCCGT+CGG | - | chr4.3:48215440-48215459 | None:intergenic | 40.0% | |
| TTCTCGATGATGCAATTCGT+AGG | + | chr4.3:48214972-48214991 | MS.gene66682:CDS | 40.0% | |
| ! | ACTGGTACCCCTTAATTTTG+CGG | - | chr4.3:48213582-48213601 | None:intergenic | 40.0% |
| ! | AGCGATTTTGTCTTGTCGAA+AGG | + | chr4.3:48213538-48213557 | MS.gene66682:intron | 40.0% |
| ! | CGATTTTGTCTTGTCGAAAG+GGG | + | chr4.3:48213540-48213559 | MS.gene66682:intron | 40.0% |
| ! | CTTTTGCAAATTGGCATGAG+AGG | + | chr4.3:48215188-48215207 | MS.gene66682:CDS | 40.0% |
| ! | GCGATTTTGTCTTGTCGAAA+GGG | + | chr4.3:48213539-48213558 | MS.gene66682:intron | 40.0% |
| ! | GCGTCTTTTCTTCAACACAA+AGG | - | chr4.3:48214873-48214892 | None:intergenic | 40.0% |
| ! | TCTGGAGATACTTTTACCGA+CGG | + | chr4.3:48215421-48215440 | MS.gene66682:CDS | 40.0% |
| ! | TGATGCACCATTTGTTGCAA+AGG | + | chr4.3:48215165-48215184 | MS.gene66682:CDS | 40.0% |
| CCTCCTCGTATACAAAACGT+GGG | - | chr4.3:48214328-48214347 | None:intergenic | 45.0% | |
| GATACGACTACGTTACGAAG+CGG | + | chr4.3:48214249-48214268 | MS.gene66682:intron | 45.0% | |
| GATGACATGGCATGCTTACT+AGG | - | chr4.3:48213493-48213512 | None:intergenic | 45.0% | |
| TCACTTCTCCAACAAGTCAG+TGG | + | chr4.3:48214199-48214218 | MS.gene66682:intron | 45.0% | |
| TCCTCCTCGTATACAAAACG+TGG | - | chr4.3:48214329-48214348 | None:intergenic | 45.0% | |
| TGTCGAAAGGGGAACTTCTA+CGG | + | chr4.3:48213551-48213570 | MS.gene66682:intron | 45.0% | |
| ! | ACGTTTTGTATACGAGGAGG+AGG | + | chr4.3:48214328-48214347 | MS.gene66682:intron | 45.0% |
| ! | CTGGAGATACTTTTACCGAC+GGG | + | chr4.3:48215422-48215441 | MS.gene66682:CDS | 45.0% |
| ! | TCCGGGCAGACATTTTCAAA+TGG | + | chr4.3:48215502-48215521 | MS.gene66682:CDS | 45.0% |
| !! | CATTGGTGATGAGAGCTATG+AGG | + | chr4.3:48215027-48215046 | MS.gene66682:CDS | 45.0% |
| !! | CTGACTTGTTGGAGAAGTGA+CGG | - | chr4.3:48214199-48214218 | None:intergenic | 45.0% |
| !! | GAAGTTGATGAAGCTCATGC+TGG | + | chr4.3:48215364-48215383 | MS.gene66682:CDS | 45.0% |
| !! | GATTTAATTATTATAATTAT+AGG | + | chr4.3:48213836-48213855 | MS.gene66682:intron | 5.0% |
| !! | TTTAAAAAAATTAAATATTC+TGG | + | chr4.3:48213302-48213321 | MS.gene66682:intron | 5.0% |
| AAACGATTCATGGCGGCAAG+GGG | - | chr4.3:48214071-48214090 | None:intergenic | 50.0% | |
| AAACGTGGGTCGTCGTAGTT+CGG | - | chr4.3:48214314-48214333 | None:intergenic | 50.0% | |
| AGAAACGATTCATGGCGGCA+AGG | - | chr4.3:48214073-48214092 | None:intergenic | 50.0% | |
| ATAAATAGAGGAGGCGGAGG+AGG | - | chr4.3:48214141-48214160 | None:intergenic | 50.0% | |
| ATCGGGAAGGAGCAGATCTA+TGG | + | chr4.3:48214612-48214631 | MS.gene66682:CDS | 50.0% | |
| ATTGACGATCTGGATCGGGA+AGG | + | chr4.3:48214599-48214618 | MS.gene66682:CDS | 50.0% | |
| CGTATCCAGAAATCCAGACG+TGG | - | chr4.3:48214235-48214254 | None:intergenic | 50.0% | |
| GAAACGATTCATGGCGGCAA+GGG | - | chr4.3:48214072-48214091 | None:intergenic | 50.0% | |
| GCATCATCGAGAACAGACTC+TGG | - | chr4.3:48214965-48214984 | None:intergenic | 50.0% | |
| TAAGATGCGGCAAGGGGTTT+GGG | - | chr4.3:48214006-48214025 | None:intergenic | 50.0% | |
| TGGATAAATAGAGGAGGCGG+AGG | - | chr4.3:48214144-48214163 | None:intergenic | 50.0% | |
| TTAAGATGCGGCAAGGGGTT+TGG | - | chr4.3:48214007-48214026 | None:intergenic | 50.0% | |
| ! | ACTTGTTGGAGAAGTGACGG+AGG | - | chr4.3:48214196-48214215 | None:intergenic | 50.0% |
| ! | CCCACGTTTTGTATACGAGG+AGG | + | chr4.3:48214325-48214344 | MS.gene66682:intron | 50.0% |
| ! | CGACCCACGTTTTGTATACG+AGG | + | chr4.3:48214322-48214341 | MS.gene66682:intron | 50.0% |
| AGCGTACGGTGGAGTAGAGA+AGG | - | chr4.3:48214118-48214137 | None:intergenic | 55.0% | |
| CGACTACGTTACGAAGCGGA+CGG | + | chr4.3:48214253-48214272 | MS.gene66682:intron | 55.0% | |
| TCACGTCTCTTGCCCAAACG+AGG | - | chr4.3:48215307-48215326 | None:intergenic | 55.0% | |
| ! | AGAAGTGACGGAGGTGGAGA+GGG | - | chr4.3:48214187-48214206 | None:intergenic | 55.0% |
| ! | TGTTGGAGAAGTGACGGAGG+TGG | - | chr4.3:48214193-48214212 | None:intergenic | 55.0% |
| !! | CAGCTGCTCCACTGACTTGT+TGG | - | chr4.3:48214210-48214229 | None:intergenic | 55.0% |
| ACGGTGGAGTAGAGAAGGCG+TGG | - | chr4.3:48214113-48214132 | None:intergenic | 60.0% | |
| ACGTTACGAAGCGGACGGAC+AGG | + | chr4.3:48214258-48214277 | MS.gene66682:intron | 60.0% | |
| CATGGCGGCAAGGGGTTTGA+TGG | - | chr4.3:48214063-48214082 | None:intergenic | 60.0% | |
| CGTTACGAAGCGGACGGACA+GGG | + | chr4.3:48214259-48214278 | MS.gene66682:intron | 60.0% | |
| CTGCGCCACGTCTGGATTTC+TGG | + | chr4.3:48214227-48214246 | MS.gene66682:intron | 60.0% | |
| CTGGGGAGAACGGAGGAAGA+TGG | - | chr4.3:48214164-48214183 | None:intergenic | 60.0% | |
| ! | GAGAAGTGACGGAGGTGGAG+AGG | - | chr4.3:48214188-48214207 | None:intergenic | 60.0% |
| ! | GGAGTAGAGAAGGCGTGGCA+AGG | - | chr4.3:48214108-48214127 | None:intergenic | 60.0% |
| ! | GTAGAGAAGGCGTGGCAAGG+AGG | - | chr4.3:48214105-48214124 | None:intergenic | 60.0% |
| GTGGAGAGGGCTGGGGAGAA+CGG | - | chr4.3:48214174-48214193 | None:intergenic | 65.0% | |
| TGACGGAGGTGGAGAGGGCT+GGG | - | chr4.3:48214182-48214201 | None:intergenic | 65.0% | |
| GACGGAGGTGGAGAGGGCTG+GGG | - | chr4.3:48214181-48214200 | None:intergenic | 70.0% | |
| GAGAGGGCTGGGGAGAACGG+AGG | - | chr4.3:48214171-48214190 | None:intergenic | 70.0% | |
| TGGAGCAGCTGCGCCACGTC+TGG | + | chr4.3:48214219-48214238 | MS.gene66682:intron | 70.0% | |
| ! | GGAGGCGGAGGAGGAGCGTA+CGG | - | chr4.3:48214132-48214151 | None:intergenic | 70.0% |
| ! | GTGACGGAGGTGGAGAGGGC+TGG | - | chr4.3:48214183-48214202 | None:intergenic | 70.0% |
| ! | GGCGGAGGAGGAGCGTACGG+TGG | - | chr4.3:48214129-48214148 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 48213172 | 48215534 | 48213172 | ID=MS.gene66682 |
| chr4.3 | mRNA | 48213172 | 48215534 | 48213172 | ID=MS.gene66682.t1;Parent=MS.gene66682 |
| chr4.3 | exon | 48213172 | 48213175 | 48213172 | ID=MS.gene66682.t1.exon1;Parent=MS.gene66682.t1 |
| chr4.3 | CDS | 48213172 | 48213175 | 48213172 | ID=cds.MS.gene66682.t1;Parent=MS.gene66682.t1 |
| chr4.3 | exon | 48214423 | 48215534 | 48214423 | ID=MS.gene66682.t1.exon2;Parent=MS.gene66682.t1 |
| chr4.3 | CDS | 48214423 | 48215534 | 48214423 | ID=cds.MS.gene66682.t1;Parent=MS.gene66682.t1 |
| Gene Sequence |
| Protein sequence |
