Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67301.t1 | NP_001280900.1 | 100 | 59 | 0 | 0 | 1 | 59 | 119 | 177 | 7.40E-24 | 119 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67301.t1 | Q9C9L2 | 98.3 | 59 | 1 | 0 | 1 | 59 | 68 | 126 | 3.0e-26 | 118.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67301.t1 | M5WM86 | 100.0 | 59 | 0 | 0 | 1 | 59 | 109 | 167 | 5.3e-24 | 119.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene67301.t1 | TF | TCP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050085 | MS.gene67301 | -0.801085 | 1.06E-48 | -1.69E-46 |
| MS.gene051452 | MS.gene67301 | -0.864513 | 1.15E-64 | -1.69E-46 |
| MS.gene051975 | MS.gene67301 | -0.854934 | 8.84E-62 | -1.69E-46 |
| MS.gene052434 | MS.gene67301 | -0.802744 | 4.85E-49 | -1.69E-46 |
| MS.gene052954 | MS.gene67301 | -0.80977 | 1.61E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene67301 | -0.804265 | 2.35E-49 | -1.69E-46 |
| MS.gene05469 | MS.gene67301 | -0.827023 | 1.97E-54 | -1.69E-46 |
| MS.gene055147 | MS.gene67301 | -0.801332 | 9.46E-49 | -1.69E-46 |
| MS.gene055155 | MS.gene67301 | -0.803551 | 3.30E-49 | -1.69E-46 |
| MS.gene055623 | MS.gene67301 | -0.817776 | 2.77E-52 | -1.69E-46 |
| MS.gene056699 | MS.gene67301 | -0.801527 | 8.63E-49 | -1.69E-46 |
| MS.gene056787 | MS.gene67301 | -0.824337 | 8.52E-54 | -1.69E-46 |
| MS.gene056986 | MS.gene67301 | -0.855984 | 4.37E-62 | -1.69E-46 |
| MS.gene057392 | MS.gene67301 | -0.808738 | 2.67E-50 | -1.69E-46 |
| MS.gene057999 | MS.gene67301 | -0.817335 | 3.48E-52 | -1.69E-46 |
| MS.gene058292 | MS.gene67301 | -0.80303 | 4.23E-49 | -1.69E-46 |
| MS.gene058595 | MS.gene67301 | -0.803583 | 3.25E-49 | -1.69E-46 |
| MS.gene059360 | MS.gene67301 | -0.809093 | 2.24E-50 | -1.69E-46 |
| MS.gene059830 | MS.gene67301 | -0.805017 | 1.64E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene67301 | -0.821846 | 3.25E-53 | -1.69E-46 |
| MS.gene060937 | MS.gene67301 | -0.821893 | 3.17E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67301.t1 | MTR_4g108370 | 100.000 | 59 | 0 | 0 | 1 | 59 | 113 | 171 | 4.23e-36 | 125 |
| MS.gene67301.t1 | MTR_5g039600 | 98.305 | 59 | 1 | 0 | 1 | 59 | 83 | 141 | 3.53e-35 | 122 |
| MS.gene67301.t1 | MTR_2g006150 | 91.525 | 59 | 5 | 0 | 1 | 59 | 146 | 204 | 1.43e-33 | 118 |
| MS.gene67301.t1 | MTR_2g006150 | 91.525 | 59 | 5 | 0 | 1 | 59 | 146 | 204 | 1.52e-33 | 119 |
| MS.gene67301.t1 | MTR_1g114380 | 80.702 | 57 | 11 | 0 | 1 | 57 | 49 | 105 | 4.13e-29 | 103 |
| MS.gene67301.t1 | MTR_1g038650 | 81.132 | 53 | 10 | 0 | 1 | 53 | 48 | 100 | 8.58e-27 | 97.1 |
| MS.gene67301.t1 | MTR_7g028160 | 89.362 | 47 | 5 | 0 | 1 | 47 | 66 | 112 | 9.42e-27 | 97.8 |
| MS.gene67301.t1 | MTR_8g033070 | 75.439 | 57 | 14 | 0 | 1 | 57 | 96 | 152 | 1.20e-25 | 95.9 |
| MS.gene67301.t1 | MTR_1g063870 | 82.979 | 47 | 8 | 0 | 1 | 47 | 65 | 111 | 5.43e-25 | 91.7 |
| MS.gene67301.t1 | MTR_1g101810 | 70.175 | 57 | 17 | 0 | 1 | 57 | 80 | 136 | 2.82e-23 | 89.4 |
| MS.gene67301.t1 | MTR_1g101810 | 70.175 | 57 | 17 | 0 | 1 | 57 | 110 | 166 | 4.44e-23 | 89.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67301.t1 | AT1G69690 | 98.305 | 59 | 1 | 0 | 1 | 59 | 68 | 126 | 1.65e-36 | 124 |
| MS.gene67301.t1 | AT3G47620 | 98.305 | 59 | 1 | 0 | 1 | 59 | 133 | 191 | 1.94e-35 | 124 |
| MS.gene67301.t1 | AT1G58100 | 89.831 | 59 | 6 | 0 | 1 | 59 | 76 | 134 | 2.74e-33 | 117 |
| MS.gene67301.t1 | AT1G72010 | 89.655 | 58 | 6 | 0 | 1 | 58 | 79 | 136 | 9.74e-33 | 115 |
| MS.gene67301.t1 | AT1G35560 | 89.831 | 59 | 6 | 0 | 1 | 59 | 71 | 129 | 4.11e-32 | 112 |
| MS.gene67301.t1 | AT5G23280 | 75.439 | 57 | 14 | 0 | 1 | 57 | 55 | 111 | 1.15e-27 | 99.4 |
| MS.gene67301.t1 | AT5G08330 | 74.138 | 58 | 15 | 0 | 1 | 58 | 47 | 104 | 2.53e-26 | 95.9 |
| MS.gene67301.t1 | AT2G45680 | 75.439 | 57 | 14 | 0 | 1 | 57 | 91 | 147 | 1.84e-25 | 95.5 |
| MS.gene67301.t1 | AT3G27010 | 89.130 | 46 | 5 | 0 | 1 | 46 | 94 | 139 | 2.13e-25 | 94.7 |
| MS.gene67301.t1 | AT3G27010 | 89.130 | 46 | 5 | 0 | 1 | 46 | 94 | 139 | 2.13e-25 | 94.7 |
| MS.gene67301.t1 | AT5G51910 | 75.439 | 57 | 14 | 0 | 1 | 57 | 73 | 129 | 8.89e-25 | 92.8 |
| MS.gene67301.t1 | AT5G51910 | 75.439 | 57 | 14 | 0 | 1 | 57 | 73 | 129 | 8.89e-25 | 92.8 |
| MS.gene67301.t1 | AT2G37000 | 81.818 | 44 | 8 | 0 | 1 | 44 | 59 | 102 | 5.98e-21 | 80.9 |
| MS.gene67301.t1 | AT5G41030 | 67.391 | 46 | 15 | 0 | 1 | 46 | 84 | 129 | 6.46e-19 | 76.6 |
| MS.gene67301.t1 | AT3G45150 | 60.465 | 43 | 17 | 0 | 1 | 43 | 33 | 75 | 5.74e-14 | 62.4 |
Find 13 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTTTCACCATCAGATTTA+TGG | 0.154484 | 8.3:-16007509 | None:intergenic |
| GTTATAGCTGCCACCGGAAC+TGG | 0.348983 | 8.3:+16007564 | MS.gene67301:CDS |
| AGCAGGTATAGTTCCAGTTC+CGG | 0.418557 | 8.3:-16007577 | None:intergenic |
| TTGAGAGATGTGAAGTTAGC+AGG | 0.457458 | 8.3:-16007594 | None:intergenic |
| TTTCAGCTTACACGCGAACT+TGG | 0.460579 | 8.3:+16007486 | MS.gene67301:CDS |
| AATATCTCACTTCGTAGCTC+AGG | 0.489424 | 8.3:+16007615 | MS.gene67301:CDS |
| TTGTTGAAGAAGCCATTCAA+TGG | 0.490318 | 8.3:-16007529 | None:intergenic |
| CCGGTGGCAGCTATAACAGC+TGG | 0.556756 | 8.3:-16007558 | None:intergenic |
| ACTCTAGCAGCACAAAGTGC+CGG | 0.564904 | 8.3:-16007462 | None:intergenic |
| CTGATGGTGAAACCATTGAA+TGG | 0.573344 | 8.3:+16007517 | MS.gene67301:CDS |
| GAACTTGGCCATAAATCTGA+TGG | 0.613525 | 8.3:+16007501 | MS.gene67301:CDS |
| CCAGCTGTTATAGCTGCCAC+CGG | 0.623678 | 8.3:+16007558 | MS.gene67301:CDS |
| AGGTATAGTTCCAGTTCCGG+TGG | 0.651482 | 8.3:-16007574 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGGTTTCACCATCAGATTTA+TGG | - | chr8.3:16007512-16007531 | None:intergenic | 35.0% | |
| TTGTTGAAGAAGCCATTCAA+TGG | - | chr8.3:16007532-16007551 | None:intergenic | 35.0% | |
| GAACTTGGCCATAAATCTGA+TGG | + | chr8.3:16007501-16007520 | MS.gene67301:CDS | 40.0% | |
| TTGAGAGATGTGAAGTTAGC+AGG | - | chr8.3:16007597-16007616 | None:intergenic | 40.0% | |
| ! | CTGATGGTGAAACCATTGAA+TGG | + | chr8.3:16007517-16007536 | MS.gene67301:CDS | 40.0% |
| AGCAGGTATAGTTCCAGTTC+CGG | - | chr8.3:16007580-16007599 | None:intergenic | 45.0% | |
| TTTCAGCTTACACGCGAACT+TGG | + | chr8.3:16007486-16007505 | MS.gene67301:CDS | 45.0% | |
| ACTCTAGCAGCACAAAGTGC+CGG | - | chr8.3:16007465-16007484 | None:intergenic | 50.0% | |
| AGGTATAGTTCCAGTTCCGG+TGG | - | chr8.3:16007577-16007596 | None:intergenic | 50.0% | |
| CCAGCTGTTATAGCTGCCAC+CGG | + | chr8.3:16007558-16007577 | MS.gene67301:CDS | 55.0% | |
| GTTATAGCTGCCACCGGAAC+TGG | + | chr8.3:16007564-16007583 | MS.gene67301:CDS | 55.0% | |
| CCGGTGGCAGCTATAACAGC+TGG | - | chr8.3:16007561-16007580 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 16007459 | 16007636 | 16007459 | ID=MS.gene67301 |
| chr8.3 | mRNA | 16007459 | 16007636 | 16007459 | ID=MS.gene67301.t1;Parent=MS.gene67301 |
| chr8.3 | exon | 16007459 | 16007636 | 16007459 | ID=MS.gene67301.t1.exon1;Parent=MS.gene67301.t1 |
| chr8.3 | CDS | 16007459 | 16007636 | 16007459 | ID=cds.MS.gene67301.t1;Parent=MS.gene67301.t1 |
| Gene Sequence |
| Protein sequence |
