AlfalfaGEDB | MS.gene68453

M. sativa cultivar XinJiangDaYe / MS.gene68453

KEGG: -
GO: -
PFAM: PF17123.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68453.t1	XP_003591673.1	77	139	4	2	3	113	4	142	1.80E-51	211.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68453.t1	Q9FG21	52.2	67	27	1	52	113	98	164	3.3e-14	79.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68453.t1	G7ZYD4	77.0	139	4	2	3	113	4	142	1.3e-51	211.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049549	MS.gene68453	0.841616	4.26E-58	-1.69E-46
MS.gene050096	MS.gene68453	0.814042	1.88E-51	-1.69E-46
MS.gene051385	MS.gene68453	0.820479	6.71E-53	-1.69E-46
MS.gene052211	MS.gene68453	0.819787	9.67E-53	-1.69E-46
MS.gene05240	MS.gene68453	0.814827	1.26E-51	-1.69E-46
MS.gene05244	MS.gene68453	0.838783	2.34E-57	-1.69E-46
MS.gene053403	MS.gene68453	0.801937	7.11E-49	-1.69E-46
MS.gene053406	MS.gene68453	0.815888	7.34E-52	-1.69E-46
MS.gene053420	MS.gene68453	0.806138	9.51E-50	-1.69E-46
MS.gene054421	MS.gene68453	0.831747	1.40E-55	-1.69E-46
MS.gene054706	MS.gene68453	0.804468	2.13E-49	-1.69E-46
MS.gene054707	MS.gene68453	0.803807	2.92E-49	-1.69E-46
MS.gene054742	MS.gene68453	0.829151	6.04E-55	-1.69E-46
MS.gene055154	MS.gene68453	0.810099	1.36E-50	-1.69E-46
MS.gene055240	MS.gene68453	0.822266	2.60E-53	-1.69E-46
MS.gene055289	MS.gene68453	-0.803663	3.13E-49	-1.69E-46
MS.gene056330	MS.gene68453	0.81567	8.21E-52	-1.69E-46
MS.gene056590	MS.gene68453	0.834689	2.59E-56	-1.69E-46
MS.gene056591	MS.gene68453	0.835611	1.52E-56	-1.69E-46
MS.gene056920	MS.gene68453	0.804266	2.35E-49	-1.69E-46
MS.gene057926	MS.gene68453	-0.800763	1.24E-48	-1.69E-46
MS.gene058603	MS.gene68453	0.801699	7.96E-49	-1.69E-46
MS.gene058840	MS.gene68453	0.805664	1.20E-49	-1.69E-46
MS.gene059955	MS.gene68453	0.802842	4.63E-49	-1.69E-46
MS.gene060087	MS.gene68453	0.808478	3.04E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68453.t1	MTR_1g090450	76.978	139	4	2	3	113	4	142	1.46e-62	188
MS.gene68453.t1	MTR_1g056350	53.425	73	30	2	42	113	69	138	1.17e-21	84.7
MS.gene68453.t1	MTR_1g090480	50.000	64	30	1	53	114	75	138	8.44e-18	74.7
MS.gene68453.t1	MTR_1g090460	51.667	60	27	1	53	110	68	127	6.10e-16	69.7
MS.gene68453.t1	MTR_1g090440	72.727	33	9	0	82	114	120	152	2.44e-13	63.2
MS.gene68453.t1	MTR_6g055030	43.860	57	31	1	59	114	106	162	1.76e-12	62.8
MS.gene68453.t1	MTR_7g079280	39.344	61	37	0	53	113	83	143	2.18e-12	60.8
MS.gene68453.t1	MTR_5g064990	40.323	62	34	2	54	113	118	178	2.49e-12	62.4
MS.gene68453.t1	MTR_2g090950	39.062	64	35	1	54	113	95	158	4.11e-12	61.2
MS.gene68453.t1	MTR_1g100737	40.323	62	34	1	56	114	98	159	5.68e-12	60.1
MS.gene68453.t1	MTR_7g076250	41.818	55	31	1	56	110	69	122	6.82e-12	59.7
MS.gene68453.t1	MTR_3g099650	38.333	60	34	2	56	113	113	171	1.63e-11	60.1
MS.gene68453.t1	MTR_2g090905	39.655	58	31	1	60	113	49	106	2.37e-11	57.0
MS.gene68453.t1	MTR_5g024730	33.784	74	46	1	40	113	70	140	2.99e-11	58.5
MS.gene68453.t1	MTR_6g087150	40.000	60	34	1	54	113	97	154	3.35e-11	59.3
MS.gene68453.t1	MTR_8g032690	57.576	33	14	0	78	110	19	51	6.68e-11	55.5
MS.gene68453.t1	MTR_1g492830	35.616	73	46	1	38	110	49	120	8.27e-11	57.4
MS.gene68453.t1	MTR_5g064980	38.333	60	34	2	56	113	114	172	9.61e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68453.t1	AT5G06490	52.941	68	25	2	52	113	98	164	2.86e-18	76.6
MS.gene68453.t1	AT2G35910	52.542	59	26	1	55	113	126	182	1.08e-16	73.2
MS.gene68453.t1	AT1G23980	36.620	71	36	2	41	111	116	177	7.68e-12	61.2
MS.gene68453.t1	AT2G46160	42.623	61	29	1	59	113	113	173	8.47e-12	60.1
MS.gene68453.t1	AT2G46160	42.623	61	29	1	59	113	113	173	8.47e-12	60.1
MS.gene68453.t1	AT5G27420	34.118	85	50	3	32	114	80	160	8.96e-12	60.8
MS.gene68453.t1	AT3G05200	41.667	60	32	2	57	114	106	164	1.39e-11	60.5
MS.gene68453.t1	AT4G28890	38.333	60	35	1	54	113	101	158	3.91e-11	59.3
MS.gene68453.t1	AT4G10160	34.940	83	48	2	31	113	56	132	4.74e-11	58.2

Find 21 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTGAGAGTATAAAAGTTTC+GGG	0.311980	1.1:+66038353	None:intergenic
ATCTGAGAGTATAAAAGTTT+CGG	0.329103	1.1:+66038352	None:intergenic
ATTAGTTGCTCTATTTGCTT+AGG	0.404219	1.1:-66038304	MS.gene68453:CDS
TACTCTCAGATTCAGAAGAA+TGG	0.427690	1.1:-66038340	MS.gene68453:CDS
ATGACGATGTGGTGGAAAAC+CGG	0.430831	1.1:+66038435	None:intergenic
TGATAAAGATGACCACAATC+TGG	0.456707	1.1:+66038247	None:intergenic
ATGTTGCTTGTGTTGATCCA+TGG	0.521110	1.1:-66038225	MS.gene68453:CDS
TTGAGGTTACTTCCAGATTG+TGG	0.535101	1.1:-66038259	MS.gene68453:CDS
TCGTCATGAAGACTCAATCA+TGG	0.545902	1.1:-66038419	MS.gene68453:CDS
ATAAAGAAAGTGACATGTTG+AGG	0.576166	1.1:-66038276	MS.gene68453:CDS
GGAATATGTAACATGAGCCA+TGG	0.584198	1.1:+66038208	None:intergenic
TCAAAACCGTTGTTGTTGTG+TGG	0.584708	1.1:+66038580	None:intergenic
AAACCGTTGTTGTTGTGTGG+AGG	0.603017	1.1:+66038583	None:intergenic
GTTGTTGTGTGGAGGTTCCG+AGG	0.639516	1.1:+66038591	None:intergenic
GTATCCTTCAAAACTTGTGT+CGG	0.641573	1.1:+66038375	None:intergenic
GAGTCTTCATGACGATGTGG+TGG	0.660756	1.1:+66038427	None:intergenic
GAGCAACAACAACGGCACCT+CGG	0.664145	1.1:-66038608	MS.gene68453:CDS
GAAGACTCAATCATGGAACA+AGG	0.664922	1.1:-66038412	MS.gene68453:CDS
GAACCTCCACACAACAACAA+CGG	0.709301	1.1:-66038586	MS.gene68453:CDS
ATTGAGTCTTCATGACGATG+TGG	0.716521	1.1:+66038424	None:intergenic
AAGAAGATGAGCAACAACAA+CGG	0.717539	1.1:-66038616	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATATCTTAATACTTGTATT+TGG	-	chr1.1:66038246-66038265	MS.gene68453:CDS	15.0%
!!	AACATTCTCAATATTTTGTC+CGG	-	chr1.1:66038333-66038352	MS.gene68453:CDS	25.0%
!!	AGTGATGATTTTCTACATAT+GGG	+	chr1.1:66038611-66038630	None:intergenic	25.0%
!!	ATCTGAGAGTATAAAAGTTT+CGG	+	chr1.1:66038438-66038457	None:intergenic	25.0%
!!	TAGTGATGATTTTCTACATA+TGG	+	chr1.1:66038612-66038631	None:intergenic	25.0%
	ATAAAGAAAGTGACATGTTG+AGG	-	chr1.1:66038511-66038530	MS.gene68453:intron	30.0%
	ATTAGTTGCTCTATTTGCTT+AGG	-	chr1.1:66038483-66038502	MS.gene68453:intron	30.0%
!	TCTGAGAGTATAAAAGTTTC+GGG	+	chr1.1:66038437-66038456	None:intergenic	30.0%
	GTATCCTTCAAAACTTGTGT+CGG	+	chr1.1:66038415-66038434	None:intergenic	35.0%
	TACTCTCAGATTCAGAAGAA+TGG	-	chr1.1:66038447-66038466	MS.gene68453:CDS	35.0%
	TGATAAAGATGACCACAATC+TGG	+	chr1.1:66038543-66038562	None:intergenic	35.0%
	TGTATTTGGTCTCGTATTCT+TGG	-	chr1.1:66038260-66038279	MS.gene68453:CDS	35.0%
!	TTTTCTACATATGGGACAAG+TGG	+	chr1.1:66038603-66038622	None:intergenic	35.0%
	ATTGAGTCTTCATGACGATG+TGG	+	chr1.1:66038366-66038385	None:intergenic	40.0%
	GAAGACTCAATCATGGAACA+AGG	-	chr1.1:66038375-66038394	MS.gene68453:CDS	40.0%
	GGAATATGTAACATGAGCCA+TGG	+	chr1.1:66038582-66038601	None:intergenic	40.0%
	TCGTCATGAAGACTCAATCA+TGG	-	chr1.1:66038368-66038387	MS.gene68453:CDS	40.0%
	TTGAGGTTACTTCCAGATTG+TGG	-	chr1.1:66038528-66038547	MS.gene68453:intron	40.0%
!	ATGTTGCTTGTGTTGATCCA+TGG	-	chr1.1:66038562-66038581	MS.gene68453:intron	40.0%
!	TCAAAACCGTTGTTGTTGTG+TGG	+	chr1.1:66038210-66038229	None:intergenic	40.0%
!!	AACAACGGTTTTGACGCAAA+TGG	-	chr1.1:66038216-66038235	MS.gene68453:CDS	40.0%
!!	ACAACGGTTTTGACGCAAAT+GGG	-	chr1.1:66038217-66038236	MS.gene68453:CDS	40.0%
!!	CATACCGACACAAGTTTTGA+AGG	-	chr1.1:66038408-66038427	MS.gene68453:CDS	40.0%
	GAACCTCCACACAACAACAA+CGG	-	chr1.1:66038201-66038220	MS.gene68453:CDS	45.0%
!	AAACCGTTGTTGTTGTGTGG+AGG	+	chr1.1:66038207-66038226	None:intergenic	45.0%
!	ATGACGATGTGGTGGAAAAC+CGG	+	chr1.1:66038355-66038374	None:intergenic	45.0%
	GAGTCTTCATGACGATGTGG+TGG	+	chr1.1:66038363-66038382	None:intergenic	50.0%
	GAGCAACAACAACGGCACCT+CGG	-	chr1.1:66038179-66038198	MS.gene68453:CDS	55.0%
	GTTGTTGTGTGGAGGTTCCG+AGG	+	chr1.1:66038199-66038218	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	66038177	66038632	66038177	ID=MS.gene68453
chr1.1	mRNA	66038177	66038632	66038177	ID=MS.gene68453.t1;Parent=MS.gene68453
chr1.1	exon	66038571	66038632	66038571	ID=MS.gene68453.t1.exon1;Parent=MS.gene68453.t1
chr1.1	CDS	66038571	66038632	66038571	ID=cds.MS.gene68453.t1;Parent=MS.gene68453.t1
chr1.1	exon	66038177	66038489	66038177	ID=MS.gene68453.t1.exon2;Parent=MS.gene68453.t1
chr1.1	CDS	66038177	66038489	66038177	ID=cds.MS.gene68453.t1;Parent=MS.gene68453.t1

Gene Sequence

>MS.gene68453

ATGAGCAACAACAACGGCACCTCGGAACCTCCACACAACAACAACGGTTTTGACGCAAATGGGTTCACATATATCTTAATACTTGTATTTGGTCTCGTATTCTTGGTTCTATCAATAATTGTTGCTTGTATTCGACTTCGTAGTTCACAAAGTCGAAACATTCTCAATATTTTGTCCGGTTTTCCACCACATCGTCATGAAGACTCAATCATGGAACAAGGACTTCGTCATCATACCGACACAAGTTTTGAAGGATACCCGAAACTTTTATACTCTCAGATTCAGAAGAATGGTTCTTCAACTGCAATTAGTTGCTCTATTTGCTTAGGAGACTATAAAGAAAGTGACATGTTGAGGTTACTTCCAGATTGTGGTCATCTTTATCATGTTGCTTGTGTTGATCCATGGCTCATGTTACATATTCCACTTGTCCCATATGTAGAAAATCATCACTAG

Protein sequence

>MS.gene68453.t1

MSNNNGTSEPPHNNNGFDANGSQSRNILNILSGFPPHRHEDSIMEQGLRHHTDTSFEGYPKLLYSQIQKNGSSTAISCSICLGDYKESDMLRLLPDCGHLYHVACVDPWLMLHIPLVPYVENHH