Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69721.t1 | XP_003601472.1 | 93.9 | 114 | 3 | 1 | 1 | 114 | 66 | 175 | 2.90E-53 | 217.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69721.t1 | G7J826 | 93.9 | 114 | 3 | 1 | 1 | 114 | 66 | 175 | 2.1e-53 | 217.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051816 | MS.gene69721 | 0.809769 | 1.61E-50 | -1.69E-46 |
| MS.gene051914 | MS.gene69721 | 0.812485 | 4.14E-51 | -1.69E-46 |
| MS.gene051955 | MS.gene69721 | 0.83946 | 1.56E-57 | -1.69E-46 |
| MS.gene052308 | MS.gene69721 | 0.864966 | 8.31E-65 | -1.69E-46 |
| MS.gene052367 | MS.gene69721 | 0.835024 | 2.13E-56 | -1.69E-46 |
| MS.gene053173 | MS.gene69721 | 0.840948 | 6.39E-58 | -1.69E-46 |
| MS.gene053348 | MS.gene69721 | 0.827362 | 1.63E-54 | -1.69E-46 |
| MS.gene054551 | MS.gene69721 | 0.860226 | 2.39E-63 | -1.69E-46 |
| MS.gene054552 | MS.gene69721 | 0.806491 | 8.02E-50 | -1.69E-46 |
| MS.gene054593 | MS.gene69721 | 0.849524 | 3.06E-60 | -1.69E-46 |
| MS.gene054662 | MS.gene69721 | 0.808637 | 2.81E-50 | -1.69E-46 |
| MS.gene055308 | MS.gene69721 | 0.853051 | 3.09E-61 | -1.69E-46 |
| MS.gene055578 | MS.gene69721 | 0.885661 | 6.71E-72 | -1.69E-46 |
| MS.gene055957 | MS.gene69721 | 0.850093 | 2.12E-60 | -1.69E-46 |
| MS.gene056041 | MS.gene69721 | 0.883941 | 2.93E-71 | -1.69E-46 |
| MS.gene05640 | MS.gene69721 | 0.851872 | 6.69E-61 | -1.69E-46 |
| MS.gene056528 | MS.gene69721 | 0.828742 | 7.59E-55 | -1.69E-46 |
| MS.gene05652 | MS.gene69721 | 0.814649 | 1.38E-51 | -1.69E-46 |
| MS.gene057458 | MS.gene69721 | 0.8277 | 1.35E-54 | -1.69E-46 |
| MS.gene058128 | MS.gene69721 | 0.843462 | 1.38E-58 | -1.69E-46 |
| MS.gene058853 | MS.gene69721 | 0.856067 | 4.13E-62 | -1.69E-46 |
| MS.gene059553 | MS.gene69721 | 0.811116 | 8.22E-51 | -1.69E-46 |
| MS.gene059616 | MS.gene69721 | 0.83454 | 2.83E-56 | -1.69E-46 |
| MS.gene060473 | MS.gene69721 | 0.841767 | 3.89E-58 | -1.69E-46 |
| MS.gene060565 | MS.gene69721 | 0.827145 | 1.84E-54 | -1.69E-46 |
| MS.gene06093 | MS.gene69721 | 0.80748 | 4.95E-50 | -1.69E-46 |
| MS.gene061099 | MS.gene69721 | 0.848262 | 6.85E-60 | -1.69E-46 |
| MS.gene061260 | MS.gene69721 | 0.844706 | 6.40E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69721.t1 | MTR_3g082070 | 93.860 | 114 | 3 | 1 | 1 | 114 | 66 | 175 | 2.85e-73 | 215 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTTTTTTTTTAAACTCTT+TGG | - | 24055:15731-15750 | MS.gene69721:intron | 15.0% |
| !! | AAATACCTTATTATTGATGA+TGG | + | 24055:15621-15640 | None:intergenic | 20.0% |
| !! | GATGAAATTGAAGTAATTAT+TGG | - | 24055:15814-15833 | MS.gene69721:CDS | 20.0% |
| !!! | AATACCTTATTATTGATGAT+GGG | + | 24055:15620-15639 | None:intergenic | 20.0% |
| ! | AATTCCTTCAATCTCAAATT+TGG | - | 24055:15512-15531 | MS.gene69721:intron | 25.0% |
| !! | AATTATTGGTGATAACTTGT+GGG | - | 24055:15828-15847 | MS.gene69721:CDS | 25.0% |
| !! | CTGTTCTGTTTTATGATATT+GGG | - | 24055:15691-15710 | MS.gene69721:intron | 25.0% |
| !! | TAATTATTGGTGATAACTTG+TGG | - | 24055:15827-15846 | MS.gene69721:CDS | 25.0% |
| !! | TCTGTTCTGTTTTATGATAT+TGG | - | 24055:15690-15709 | MS.gene69721:intron | 25.0% |
| !!! | ATACCTTATTATTGATGATG+GGG | + | 24055:15619-15638 | None:intergenic | 25.0% |
| TAACCCCATCATCAATAATA+AGG | - | 24055:15613-15632 | MS.gene69721:intron | 30.0% | |
| ! | GAAACCAAATTTGAGATTGA+AGG | + | 24055:15519-15538 | None:intergenic | 30.0% |
| AAAACAGTTCAAGAGATGGA+AGG | - | 24055:15898-15917 | MS.gene69721:CDS | 35.0% | |
| AAGAAACTCTCCTATGACAT+TGG | - | 24055:15428-15447 | MS.gene69721:CDS | 35.0% | |
| ATGTCATAGGAGAGTTTCTT+TGG | + | 24055:15428-15447 | None:intergenic | 35.0% | |
| CTACCAATGTCTTTCAAAGA+TGG | - | 24055:15950-15969 | MS.gene69721:CDS | 35.0% | |
| CTACTATGTGATACAGAGAT+GGG | - | 24055:15853-15872 | MS.gene69721:CDS | 35.0% | |
| CTTCAAAACAGTTCAAGAGA+TGG | - | 24055:15894-15913 | MS.gene69721:CDS | 35.0% | |
| TACTATGTGATACAGAGATG+GGG | - | 24055:15854-15873 | MS.gene69721:CDS | 35.0% | |
| ! | TCTCCATCTTTGAAAGACAT+TGG | + | 24055:15956-15975 | None:intergenic | 35.0% |
| !! | ATTTGATCCTTGAAGGTGTT+GGG | + | 24055:15586-15605 | None:intergenic | 35.0% |
| CATGCACATTTGATCCTTGA+AGG | + | 24055:15593-15612 | None:intergenic | 40.0% | |
| GCTACTATGTGATACAGAGA+TGG | - | 24055:15852-15871 | MS.gene69721:CDS | 40.0% | |
| ! | CATTTGATCCTTGAAGGTGT+TGG | + | 24055:15587-15606 | None:intergenic | 40.0% |
| !! | ACAAGGTTGGTTCTAAGCTT+AGG | - | 24055:15474-15493 | MS.gene69721:CDS | 40.0% |
| !! | TTTGATCCTTGAAGGTGTTG+GGG | + | 24055:15585-15604 | None:intergenic | 40.0% |
| AGATGCAGTGTCAACAAGGT+TGG | - | 24055:15461-15480 | MS.gene69721:CDS | 45.0% | |
| AGCTAGATGCAGTGTCAACA+AGG | - | 24055:15457-15476 | MS.gene69721:CDS | 45.0% | |
| AGCTCCTAAGCCAATGTCAT+AGG | + | 24055:15441-15460 | None:intergenic | 45.0% | |
| ATTTCTCCCCAACACCTTCA+AGG | - | 24055:15576-15595 | MS.gene69721:intron | 45.0% | |
| GGTAGCATCACATGAGCAAT+GGG | + | 24055:15935-15954 | None:intergenic | 45.0% | |
| GTAGCATCACATGAGCAATG+GGG | + | 24055:15934-15953 | None:intergenic | 45.0% | |
| TGGTAGCATCACATGAGCAA+TGG | + | 24055:15936-15955 | None:intergenic | 45.0% | |
| !! | CTCTCCTATGACATTGGCTT+AGG | - | 24055:15434-15453 | MS.gene69721:CDS | 45.0% |
| ATGTGATACAGAGATGGGGC+AGG | - | 24055:15858-15877 | MS.gene69721:CDS | 50.0% | |
| TGTGATACAGAGATGGGGCA+GGG | - | 24055:15859-15878 | MS.gene69721:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 24055 | gene | 15404 | 15982 | 15404 | ID=MS.gene69721 |
| 24055 | mRNA | 15404 | 15982 | 15404 | ID=MS.gene69721.t1;Parent=MS.gene69721 |
| 24055 | exon | 15752 | 15982 | 15752 | ID=MS.gene69721.t1.exon1;Parent=MS.gene69721.t1 |
| 24055 | CDS | 15752 | 15982 | 15752 | ID=cds.MS.gene69721.t1;Parent=MS.gene69721.t1 |
| 24055 | exon | 15404 | 15517 | 15404 | ID=MS.gene69721.t1.exon2;Parent=MS.gene69721.t1 |
| 24055 | CDS | 15404 | 15517 | 15404 | ID=cds.MS.gene69721.t1;Parent=MS.gene69721.t1 |
| Gene Sequence |
| Protein sequence |
