AlfalfaGEDB | MS.gene69758

M. sativa cultivar XinJiangDaYe / MS.gene69758

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69758.t1	XP_003601436.2	79.6	98	20	0	1	98	101	198	1.40E-38	168.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69758.t1	G7J7C7	79.6	98	20	0	1	98	101	198	9.9e-39	168.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene69758	MS.gene71284	PPI
MS.gene69758	MS.gene71531	PPI
MS.gene044361	MS.gene69758	PPI
MS.gene69758	MS.gene71282	PPI
MS.gene77466	MS.gene69758	PPI
MS.gene044370	MS.gene69758	PPI
MS.gene69758	MS.gene71524	PPI
MS.gene69758	MS.gene71528	PPI
MS.gene25704	MS.gene69758	PPI
MS.gene69758	MS.gene045214	PPI
MS.gene69758	MS.gene77464	PPI
MS.gene70443	MS.gene69758	PPI
MS.gene69758	MS.gene044326	PPI
MS.gene79115	MS.gene69758	PPI
MS.gene70430	MS.gene69758	PPI
MS.gene69758	MS.gene70424	PPI
MS.gene69758	MS.gene049263	PPI
MS.gene044376	MS.gene69758	PPI
MS.gene044363	MS.gene69758	PPI
MS.gene69758	MS.gene70427	PPI
MS.gene69758	MS.gene70476	PPI
MS.gene69758	MS.gene77468	PPI
MS.gene77472	MS.gene69758	PPI
MS.gene70418	MS.gene69758	PPI
MS.gene69758	MS.gene044323	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69758.t1	MTR_3g080680	82.474	97	16	1	2	98	103	198	3.32e-55	170
MS.gene69758.t1	MTR_3g082420	53.465	101	43	3	2	98	121	221	3.78e-26	96.7
MS.gene69758.t1	MTR_3g082340	53.465	101	43	3	2	98	122	222	8.27e-26	95.9
MS.gene69758.t1	MTR_8g461300	50.485	103	45	2	2	98	96	198	5.98e-24	90.5
MS.gene69758.t1	MTR_3g083050	45.000	100	47	5	2	100	116	208	1.04e-15	69.3
MS.gene69758.t1	MTR_3g083030	45.000	100	47	5	2	100	139	231	1.30e-15	69.3
MS.gene69758.t1	MTR_4g055800	45.882	85	31	3	2	82	200	273	2.63e-14	66.6
MS.gene69758.t1	MTR_3g083150	43.750	96	49	3	6	99	120	212	1.34e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 23 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATAGATTCTACCATTAATA+AGG	0.262811	3.4:-77297199	None:intergenic
TGTACCCATGCCCTTATTAA+TGG	0.282280	3.4:+77297188	MS.gene69758:CDS
TGGAGTGTCCTTCGAGAATA+GGG	0.410247	3.4:-77297003	None:intergenic
TTGGAGTGTCCTTCGAGAAT+AGG	0.428317	3.4:-77297004	None:intergenic
GCTTGGAAAGTTGTTAAAGC+AGG	0.466031	3.4:-77297266	None:intergenic
ATAGATTCTACCATTAATAA+GGG	0.476198	3.4:-77297198	None:intergenic
GAGAATAGGGCGAACATCAT+CGG	0.483809	3.4:-77296990	None:intergenic
TTCTACCATTAATAAGGGCA+TGG	0.508172	3.4:-77297193	None:intergenic
AGGCCATGAAACACAAAAGC+TGG	0.508489	3.4:-77297158	None:intergenic
AGGACACTCCAAGCTTGCAT+TGG	0.517715	3.4:+77297015	MS.gene69758:CDS
GTAATAGTCCAATGCAAGCT+TGG	0.525102	3.4:-77297023	None:intergenic
CTTGGAAAGTTGTTAAAGCA+GGG	0.540365	3.4:-77297265	None:intergenic
AACATCATCGGAAATGTGAA+GGG	0.573072	3.4:-77296978	None:intergenic
GAACATCATCGGAAATGTGA+AGG	0.574469	3.4:-77296979	None:intergenic
GAACACAGCATACTTCAGCT+TGG	0.589049	3.4:-77297283	None:intergenic
TCTACCATTAATAAGGGCAT+GGG	0.593250	3.4:-77297192	None:intergenic
AGTTGTTAAAGCAGGGGTGT+CGG	0.599691	3.4:-77297258	None:intergenic
GCACACTCTCTGATCTCAGG+TGG	0.621362	3.4:-77297345	None:intergenic
TTGGAAAGTTGTTAAAGCAG+GGG	0.623852	3.4:-77297264	None:intergenic
GATGTTCGCCCTATTCTCGA+AGG	0.637823	3.4:+77296995	MS.gene69758:CDS
GGGCATGGGTACACTTAACA+AGG	0.642669	3.4:-77297178	None:intergenic
TGTTCGAGATAAACAACCTG+TGG	0.664849	3.4:+77297301	MS.gene69758:CDS
ACATCATCGGAAATGTGAAG+GGG	0.674764	3.4:-77296977	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTCTTCATTATATTATATTA+GGG	+	chr3.4:77297134-77297153	MS.gene69758:intron	10.0%
!!	CTTCTTCATTATATTATATT+AGG	+	chr3.4:77297133-77297152	MS.gene69758:intron	15.0%
!!	AATAGATTCTACCATTAATA+AGG	-	chr3.4:77297202-77297221	None:intergenic	20.0%
!!	AGAAGAACAATTAATTAGTT+TGG	-	chr3.4:77297118-77297137	None:intergenic	20.0%
!!	ATAGATTCTACCATTAATAA+GGG	-	chr3.4:77297201-77297220	None:intergenic	20.0%
!!!	CCTGATTACTGTTTTTTTCA+CGG	-	chr3.4:77297048-77297067	None:intergenic	30.0%
	AACATCATCGGAAATGTGAA+GGG	-	chr3.4:77296981-77297000	None:intergenic	35.0%
	CCGTGAAAAAAACAGTAATC+AGG	+	chr3.4:77297045-77297064	MS.gene69758:CDS	35.0%
	CTTGGAAAGTTGTTAAAGCA+GGG	-	chr3.4:77297268-77297287	None:intergenic	35.0%
	TTCTACCATTAATAAGGGCA+TGG	-	chr3.4:77297196-77297215	None:intergenic	35.0%
	TTGGAAAGTTGTTAAAGCAG+GGG	-	chr3.4:77297267-77297286	None:intergenic	35.0%
!	AAGATCATCTTCAGCTTTTG+CGG	-	chr3.4:77297237-77297256	None:intergenic	35.0%
!	TCTACCATTAATAAGGGCAT+GGG	-	chr3.4:77297195-77297214	None:intergenic	35.0%
	ACATCATCGGAAATGTGAAG+GGG	-	chr3.4:77296980-77296999	None:intergenic	40.0%
	GAACATCATCGGAAATGTGA+AGG	-	chr3.4:77296982-77297001	None:intergenic	40.0%
	GCTTGGAAAGTTGTTAAAGC+AGG	-	chr3.4:77297269-77297288	None:intergenic	40.0%
	GTAATAGTCCAATGCAAGCT+TGG	-	chr3.4:77297026-77297045	None:intergenic	40.0%
	TGTACCCATGCCCTTATTAA+TGG	+	chr3.4:77297188-77297207	MS.gene69758:CDS	40.0%
	TGTTCGAGATAAACAACCTG+TGG	+	chr3.4:77297301-77297320	MS.gene69758:CDS	40.0%
!	ATCTTCAGCTTTTGCGGTAA+AGG	-	chr3.4:77297231-77297250	None:intergenic	40.0%
	AGGCCATGAAACACAAAAGC+TGG	-	chr3.4:77297161-77297180	None:intergenic	45.0%
	GAACACAGCATACTTCAGCT+TGG	-	chr3.4:77297286-77297305	None:intergenic	45.0%
	GAGAATAGGGCGAACATCAT+CGG	-	chr3.4:77296993-77297012	None:intergenic	45.0%
!	AGTTGTTAAAGCAGGGGTGT+CGG	-	chr3.4:77297261-77297280	None:intergenic	45.0%
!	GGTCCAGCTTTTGTGTTTCA+TGG	+	chr3.4:77297155-77297174	MS.gene69758:CDS	45.0%
!	TGGAGTGTCCTTCGAGAATA+GGG	-	chr3.4:77297006-77297025	None:intergenic	45.0%
!	TTGGAGTGTCCTTCGAGAAT+AGG	-	chr3.4:77297007-77297026	None:intergenic	45.0%
!!	ATGGCACACTTTTTGACCAC+AGG	-	chr3.4:77297320-77297339	None:intergenic	45.0%
	AGGACACTCCAAGCTTGCAT+TGG	+	chr3.4:77297015-77297034	MS.gene69758:CDS	50.0%
	GATGTTCGCCCTATTCTCGA+AGG	+	chr3.4:77296995-77297014	MS.gene69758:CDS	50.0%
	GGGCATGGGTACACTTAACA+AGG	-	chr3.4:77297181-77297200	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	77296962	77297352	77296962	ID=MS.gene69758
chr3.4	mRNA	77296962	77297352	77296962	ID=MS.gene69758.t1;Parent=MS.gene69758
chr3.4	exon	77296962	77297066	77296962	ID=MS.gene69758.t1.exon1;Parent=MS.gene69758.t1
chr3.4	CDS	77296962	77297066	77296962	ID=cds.MS.gene69758.t1;Parent=MS.gene69758.t1
chr3.4	exon	77297155	77297352	77297155	ID=MS.gene69758.t1.exon2;Parent=MS.gene69758.t1
chr3.4	CDS	77297155	77297352	77297155	ID=cds.MS.gene69758.t1;Parent=MS.gene69758.t1

Gene Sequence

>MS.gene69758

ATGGGCGGTATCATACCCCTTCACATTTCCGATGATGTTCGCCCTATTCTCGAAGGACACTCCAAGCTTGCATTGGACTATTACCGTGAAAAAAACAGTAATCAGGTTCGATACTAAGAGAATGAATATTATTGTATGTTGTTTATTCATTAACCAAACTAATTAATTGTTCTTCTTCATTATATTATATTAGGGTCCAGCTTTTGTGTTTCATGGCCTTGTTAAGTGTACCCATGCCCTTATTAATGGTAGAATCTATTACATTACCTTTACCGCAAAAGCTGAAGATGATCTTTCCGACACCCCTGCTTTAACAACTTTCCAAGCTGAAGTATGCTGTGTTCGAGATAAACAACCTGTGGTCAAAAAGTGTGCCATTAAAACCACCTGA

Protein sequence

>MS.gene69758.t1

MGGIIPLHISDDVRPILEGHSKLALDYYREKNSNQGPAFVFHGLVKCTHALINGRIYYITFTAKAEDDLSDTPALTTFQAEVCCVRDKQPVVKKCAIKTT