Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70431.t1 | XP_003601497.1 | 42.5 | 167 | 81 | 5 | 7 | 158 | 57 | 223 | 2.70E-20 | 108.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70431.t1 | G7J851 | 42.5 | 167 | 81 | 5 | 7 | 158 | 57 | 223 | 1.9e-20 | 108.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene79115 | MS.gene70431 | PPI |
| MS.gene044370 | MS.gene70431 | PPI |
| MS.gene70431 | MS.gene044326 | PPI |
| MS.gene70443 | MS.gene70431 | PPI |
| MS.gene70431 | MS.gene70427 | PPI |
| MS.gene70431 | MS.gene70476 | PPI |
| MS.gene044363 | MS.gene70431 | PPI |
| MS.gene70430 | MS.gene70431 | PPI |
| MS.gene70431 | MS.gene049263 | PPI |
| MS.gene044376 | MS.gene70431 | PPI |
| MS.gene70431 | MS.gene77468 | PPI |
| MS.gene70431 | MS.gene71531 | PPI |
| MS.gene70431 | MS.gene044323 | PPI |
| MS.gene77472 | MS.gene70431 | PPI |
| MS.gene70418 | MS.gene70431 | PPI |
| MS.gene70431 | MS.gene71284 | PPI |
| MS.gene70431 | MS.gene045214 | PPI |
| MS.gene77466 | MS.gene70431 | PPI |
| MS.gene25704 | MS.gene70431 | PPI |
| MS.gene70431 | MS.gene71282 | PPI |
| MS.gene70431 | MS.gene71524 | PPI |
| MS.gene70431 | MS.gene71528 | PPI |
| MS.gene70431 | MS.gene70424 | PPI |
| MS.gene044361 | MS.gene70431 | PPI |
| MS.gene70431 | MS.gene77464 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70431.t1 | MTR_3g082340 | 41.916 | 167 | 82 | 5 | 7 | 158 | 57 | 223 | 2.80e-32 | 114 |
| MS.gene70431.t1 | MTR_3g082420 | 39.378 | 193 | 81 | 7 | 2 | 158 | 30 | 222 | 4.74e-32 | 114 |
| MS.gene70431.t1 | MTR_3g080680 | 46.541 | 159 | 66 | 6 | 16 | 158 | 44 | 199 | 8.51e-29 | 105 |
| MS.gene70431.t1 | MTR_3g083080 | 43.571 | 140 | 73 | 3 | 18 | 157 | 72 | 205 | 3.15e-26 | 99.0 |
| MS.gene70431.t1 | MTR_3g083030 | 45.390 | 141 | 68 | 5 | 18 | 157 | 97 | 229 | 9.07e-24 | 93.2 |
| MS.gene70431.t1 | MTR_3g083050 | 45.390 | 141 | 68 | 5 | 18 | 157 | 74 | 206 | 1.52e-23 | 92.0 |
| MS.gene70431.t1 | MTR_8g461300 | 43.066 | 137 | 65 | 5 | 32 | 157 | 64 | 198 | 1.06e-18 | 79.0 |
| MS.gene70431.t1 | MTR_4g055800 | 35.849 | 159 | 68 | 4 | 11 | 144 | 127 | 276 | 5.02e-17 | 76.3 |
| MS.gene70431.t1 | MTR_3g083150 | 37.600 | 125 | 69 | 5 | 38 | 157 | 91 | 211 | 1.51e-13 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCGGTTGTGATCCCAATTC+AGG | 0.283996 | 3.1:+66241216 | None:intergenic |
| TCTCTCCGACCTTGCTTTGA+AGG | 0.298998 | 3.1:-66240893 | MS.gene70431:CDS |
| CAAAATGTCGAAACCTGAAT+TGG | 0.303444 | 3.1:-66241229 | None:intergenic |
| CAACTTTCCAGGCTGAAGTA+TGG | 0.336254 | 3.1:-66240632 | MS.gene70431:CDS |
| GTCAGCGATTAGTTCTTCTA+TGG | 0.365862 | 3.1:+66241137 | None:intergenic |
| CCATGCTAGAACAACTTTCC+AGG | 0.422290 | 3.1:-66240643 | MS.gene70431:CDS |
| ACTGCTATTGCCTGTGGTGG+TGG | 0.454132 | 3.1:-66240720 | MS.gene70431:CDS |
| GGATTACAATCAAAAGAATC+AGG | 0.463360 | 3.1:-66240872 | MS.gene70431:intron |
| TCCGAATCGTGTTTCGATTG+CGG | 0.463704 | 3.1:+66241198 | None:intergenic |
| AAGATCTTCGGTTAAGTCAA+TGG | 0.464314 | 3.1:+66240933 | None:intergenic |
| CCACATAGCTCTAGAACTGC+TGG | 0.483421 | 3.1:+66240967 | None:intergenic |
| AAAATGTCGAAACCTGAATT+GGG | 0.487541 | 3.1:-66241228 | MS.gene70431:CDS |
| ACCGCAATCGAAACACGATT+CGG | 0.495086 | 3.1:-66241199 | MS.gene70431:CDS |
| AACTTTCCAGGCTGAAGTAT+GGG | 0.495182 | 3.1:-66240631 | MS.gene70431:CDS |
| CGACGACGATGACGAGTTTG+CGG | 0.511755 | 3.1:-66241172 | MS.gene70431:CDS |
| TCGGAGAGAGCAACGAGAGT+AGG | 0.517372 | 3.1:+66240907 | None:intergenic |
| AGATCTTCGGTTAAGTCAAT+GGG | 0.517721 | 3.1:+66240934 | None:intergenic |
| CGAGTTTGCGGAAGAGAAAG+AGG | 0.530206 | 3.1:-66241160 | MS.gene70431:CDS |
| GAGAGTAGGACGAAGATCTT+CGG | 0.531389 | 3.1:+66240921 | None:intergenic |
| GATTGTAATCCTTCAAAGCA+AGG | 0.537388 | 3.1:+66240884 | None:intergenic |
| AGTTGTTCTAGCATGGCTGT+TGG | 0.551129 | 3.1:+66240650 | None:intergenic |
| GTTCTAGCATGGCTGTTGGA+AGG | 0.554237 | 3.1:+66240654 | None:intergenic |
| CATCATCCCATACTTCAGCC+TGG | 0.561199 | 3.1:+66240625 | None:intergenic |
| ATACAAATCACCACCACCAC+AGG | 0.568911 | 3.1:+66240710 | None:intergenic |
| CCTGGAAAGTTGTTCTAGCA+TGG | 0.574216 | 3.1:+66240643 | None:intergenic |
| ACCACTGCTATTGCCTGTGG+TGG | 0.600684 | 3.1:-66240723 | MS.gene70431:CDS |
| GGCACATGATATGACCTCAT+CGG | 0.602813 | 3.1:+66240593 | None:intergenic |
| TGTACCACTGCTATTGCCTG+TGG | 0.630788 | 3.1:-66240726 | MS.gene70431:CDS |
| CCAGCAGTTCTAGAGCTATG+TGG | 0.642746 | 3.1:-66240967 | MS.gene70431:CDS |
| TAGCAGTGGTACACTTGACA+AGG | 0.661404 | 3.1:+66240736 | None:intergenic |
| ACCACCACAGGCAATAGCAG+TGG | 0.671651 | 3.1:+66240722 | None:intergenic |
| GTAATCCTTCAAAGCAAGGT+CGG | 0.676410 | 3.1:+66240888 | None:intergenic |
| TGATGATGATGATGCCGATG+AGG | 0.684211 | 3.1:-66240607 | MS.gene70431:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGTTCTTCTTAATTATT+AGG | - | chr3.1:66241025-66241044 | MS.gene70431:intron | 15.0% |
| !! | ATTGTTCTTCTTAATTATTA+GGG | - | chr3.1:66241026-66241045 | MS.gene70431:intron | 15.0% |
| ATAGATAGTTTCGTACACTT+AGG | + | chr3.1:66240715-66240734 | None:intergenic | 30.0% | |
| GGATTACAATCAAAAGAATC+AGG | - | chr3.1:66240934-66240953 | MS.gene70431:CDS | 30.0% | |
| AAGATCTTCGGTTAAGTCAA+TGG | + | chr3.1:66240876-66240895 | None:intergenic | 35.0% | |
| AGATCTTCGGTTAAGTCAAT+GGG | + | chr3.1:66240875-66240894 | None:intergenic | 35.0% | |
| GATTGTAATCCTTCAAAGCA+AGG | + | chr3.1:66240925-66240944 | None:intergenic | 35.0% | |
| AACTTTCCAGGCTGAAGTAT+GGG | - | chr3.1:66241175-66241194 | MS.gene70431:CDS | 40.0% | |
| GTAATCCTTCAAAGCAAGGT+CGG | + | chr3.1:66240921-66240940 | None:intergenic | 40.0% | |
| ! | GTCAGCGATTAGTTCTTCTA+TGG | + | chr3.1:66240672-66240691 | None:intergenic | 40.0% |
| ! | TGATTCTGCTGACTATACAC+AGG | - | chr3.1:66240796-66240815 | MS.gene70431:intron | 40.0% |
| ACCGCAATCGAAACACGATT+CGG | - | chr3.1:66240607-66240626 | MS.gene70431:CDS | 45.0% | |
| AGTTGTTCTAGCATGGCTGT+TGG | + | chr3.1:66241159-66241178 | None:intergenic | 45.0% | |
| ATACAAATCACCACCACCAC+AGG | + | chr3.1:66241099-66241118 | None:intergenic | 45.0% | |
| CAACTTTCCAGGCTGAAGTA+TGG | - | chr3.1:66241174-66241193 | MS.gene70431:CDS | 45.0% | |
| CCATGCTAGAACAACTTTCC+AGG | - | chr3.1:66241163-66241182 | MS.gene70431:CDS | 45.0% | |
| CCTGGAAAGTTGTTCTAGCA+TGG | + | chr3.1:66241166-66241185 | None:intergenic | 45.0% | |
| GAGAGTAGGACGAAGATCTT+CGG | + | chr3.1:66240888-66240907 | None:intergenic | 45.0% | |
| GGCACATGATATGACCTCAT+CGG | + | chr3.1:66241216-66241235 | None:intergenic | 45.0% | |
| TAGCAGTGGTACACTTGACA+AGG | + | chr3.1:66241073-66241092 | None:intergenic | 45.0% | |
| TCCGAATCGTGTTTCGATTG+CGG | + | chr3.1:66240611-66240630 | None:intergenic | 45.0% | |
| ! | TGATGATGATGATGCCGATG+AGG | - | chr3.1:66241199-66241218 | MS.gene70431:CDS | 45.0% |
| CATCATCCCATACTTCAGCC+TGG | + | chr3.1:66241184-66241203 | None:intergenic | 50.0% | |
| CGAGTTTGCGGAAGAGAAAG+AGG | - | chr3.1:66240646-66240665 | MS.gene70431:CDS | 50.0% | |
| GTTCTAGCATGGCTGTTGGA+AGG | + | chr3.1:66241155-66241174 | None:intergenic | 50.0% | |
| TCTCTCCGACCTTGCTTTGA+AGG | - | chr3.1:66240913-66240932 | MS.gene70431:CDS | 50.0% | |
| TGCGGTTGTGATCCCAATTC+AGG | + | chr3.1:66240593-66240612 | None:intergenic | 50.0% | |
| TGTACCACTGCTATTGCCTG+TGG | - | chr3.1:66241080-66241099 | MS.gene70431:intron | 50.0% | |
| !! | CCACATAGCTCTAGAACTGC+TGG | + | chr3.1:66240842-66240861 | None:intergenic | 50.0% |
| !! | CCAGCAGTTCTAGAGCTATG+TGG | - | chr3.1:66240839-66240858 | MS.gene70431:intron | 50.0% |
| ACCACCACAGGCAATAGCAG+TGG | + | chr3.1:66241087-66241106 | None:intergenic | 55.0% | |
| ACCACTGCTATTGCCTGTGG+TGG | - | chr3.1:66241083-66241102 | MS.gene70431:intron | 55.0% | |
| ACTGCTATTGCCTGTGGTGG+TGG | - | chr3.1:66241086-66241105 | MS.gene70431:intron | 55.0% | |
| CGACGACGATGACGAGTTTG+CGG | - | chr3.1:66240634-66240653 | MS.gene70431:CDS | 55.0% | |
| TCGGAGAGAGCAACGAGAGT+AGG | + | chr3.1:66240902-66240921 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 66240581 | 66241247 | 66240581 | ID=MS.gene70431 |
| chr3.1 | mRNA | 66240581 | 66241247 | 66240581 | ID=MS.gene70431.t1;Parent=MS.gene70431 |
| chr3.1 | exon | 66241110 | 66241247 | 66241110 | ID=MS.gene70431.t1.exon1;Parent=MS.gene70431.t1 |
| chr3.1 | CDS | 66241110 | 66241247 | 66241110 | ID=cds.MS.gene70431.t1;Parent=MS.gene70431.t1 |
| chr3.1 | exon | 66240873 | 66241010 | 66240873 | ID=MS.gene70431.t1.exon2;Parent=MS.gene70431.t1 |
| chr3.1 | CDS | 66240873 | 66241010 | 66240873 | ID=cds.MS.gene70431.t1;Parent=MS.gene70431.t1 |
| chr3.1 | exon | 66240581 | 66240781 | 66240581 | ID=MS.gene70431.t1.exon3;Parent=MS.gene70431.t1 |
| chr3.1 | CDS | 66240581 | 66240781 | 66240581 | ID=cds.MS.gene70431.t1;Parent=MS.gene70431.t1 |
| Gene Sequence |
| Protein sequence |
